Swain Lab microscope experiment log file
Swain lab microscope control software - MultiDGUI commit number:64cb2d3d741b085823d261c4da0b596c4b9079e0
Warning! - there are uncommitted changes - software may be different from the above commit
11-Jul-2019
Microscope name is: Batgirl
Acquisition settings are saved in:
E:/AcquisitionDataBatgirl\Swain Lab/Alan/RAW DATA/2019/Jul/11-Jul-2019/aggregates_CTP_switch_2_0glu_0_0glu_URA7young_URA8young_URA8old_01/aggregates_CTP_switch_2_0glu_0_0glu_URA7young_URA8young_URA8oldAcq.txt
Experiment details:
Aim:   Strain:   Comments:   
Microscope setup for used channels:
Brightfield:
White LED
->(Polariser + Prism + condenser)]
->Filter block:[GFP/mCherry (59022) exciter,GFP/mCherry (59022) dichroic,no emission filter]
->Emission filter wheel:[No filter in emission wheel]
GFP:
470nm LED
->Combiner cube:[,  495lp dichroic->(425LP dichroic)]
->Filter block:[GFP/mCherry (59022) exciter,GFP/mCherry (59022) dichroic,no emission filter]
->Emission filter wheel:[520/40 emission filter]
Micromanager config file:C:\Users\Public\Microscope control\Micromanager config files\MM2Batgirl2_11_18.txt
Omero project:
ProteinAggregates
Omero tags:
11-Jul-2019,Batgirl,Alan,GFP,3 chamber,URA7-GFP,URA8-GFP,glucose,
Omero tag descriptions:
,,,,,Strain,Strain,
Experiment started at:11-Jul-2019 16:27:34



------Time point_1------

Memory available for all arrays: 61043798016

Memory used by Matlab: 2235244544

Switching pumps at 11-Jul-2019 16:27:34. Fast infusion/withdrawal step: Rate=100. Volume=50

Pump switch complete at:11-Jul-2019 16:27:34

Dominant pump is now: COM7 pumping at 0ul/min

Dominant medium is now: 0% glucose in SC

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:27:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-4.15

Channel configuration set to: Brightfield: 11-Jul-2019 16:27:36

Channel:Brightfield set at:11-Jul-2019 16:27:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.1501. 11-Jul-2019 16:27:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:27:36

Channel configuration set to: GFP: 11-Jul-2019 16:27:38

Channel:GFP set at:11-Jul-2019 16:27:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.1251. 11-Jul-2019 16:27:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:27:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:27:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-4.8

Channel configuration set to: Brightfield: 11-Jul-2019 16:27:41

Channel:Brightfield set at:11-Jul-2019 16:27:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.0751. 11-Jul-2019 16:27:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:27:41

Channel configuration set to: GFP: 11-Jul-2019 16:27:42

Channel:GFP set at:11-Jul-2019 16:27:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.0751. 11-Jul-2019 16:27:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:27:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:27:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-4.975

Channel configuration set to: Brightfield: 11-Jul-2019 16:27:46

Channel:Brightfield set at:11-Jul-2019 16:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.9251. 11-Jul-2019 16:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:27:46

Channel configuration set to: GFP: 11-Jul-2019 16:27:47

Channel:GFP set at:11-Jul-2019 16:27:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.9251. 11-Jul-2019 16:27:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:27:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:27:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-4.075

Channel configuration set to: Brightfield: 11-Jul-2019 16:27:50

Channel:Brightfield set at:11-Jul-2019 16:27:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.6251. 11-Jul-2019 16:27:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:27:51

Channel configuration set to: GFP: 11-Jul-2019 16:27:52

Channel:GFP set at:11-Jul-2019 16:27:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.6251. 11-Jul-2019 16:27:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:27:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:27:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-3.075

Channel configuration set to: Brightfield: 11-Jul-2019 16:27:55

Channel:Brightfield set at:11-Jul-2019 16:27:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8751. 11-Jul-2019 16:27:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:27:56

Channel configuration set to: GFP: 11-Jul-2019 16:27:57

Channel:GFP set at:11-Jul-2019 16:27:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8501. 11-Jul-2019 16:27:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:27:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:27:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-4.625

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:00

Channel:Brightfield set at:11-Jul-2019 16:28:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 16:28:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:01

Channel configuration set to: GFP: 11-Jul-2019 16:28:02

Channel:GFP set at:11-Jul-2019 16:28:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 16:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-2.225

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:06

Channel:Brightfield set at:11-Jul-2019 16:28:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6501. 11-Jul-2019 16:28:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:06

Channel configuration set to: GFP: 11-Jul-2019 16:28:07

Channel:GFP set at:11-Jul-2019 16:28:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6751. 11-Jul-2019 16:28:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-1.825

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:10

Channel:Brightfield set at:11-Jul-2019 16:28:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.0501. 11-Jul-2019 16:28:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:11

Channel configuration set to: GFP: 11-Jul-2019 16:28:12

Channel:GFP set at:11-Jul-2019 16:28:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.0501. 11-Jul-2019 16:28:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-2.175

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:16

Channel:Brightfield set at:11-Jul-2019 16:28:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3501. 11-Jul-2019 16:28:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:16

Channel configuration set to: GFP: 11-Jul-2019 16:28:17

Channel:GFP set at:11-Jul-2019 16:28:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3501. 11-Jul-2019 16:28:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-2.5

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:21

Channel:Brightfield set at:11-Jul-2019 16:28:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 16:28:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:21

Channel configuration set to: GFP: 11-Jul-2019 16:28:22

Channel:GFP set at:11-Jul-2019 16:28:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 16:28:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-2.2

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:25

Channel:Brightfield set at:11-Jul-2019 16:28:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 11-Jul-2019 16:28:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:26

Channel configuration set to: GFP: 11-Jul-2019 16:28:27

Channel:GFP set at:11-Jul-2019 16:28:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 16:28:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-1.55

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:30

Channel:Brightfield set at:11-Jul-2019 16:28:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 16:28:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:30

Channel configuration set to: GFP: 11-Jul-2019 16:28:31

Channel:GFP set at:11-Jul-2019 16:28:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 16:28:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-1.575

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:35

Channel:Brightfield set at:11-Jul-2019 16:28:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.9251. 11-Jul-2019 16:28:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:35

Channel configuration set to: GFP: 11-Jul-2019 16:28:37

Channel:GFP set at:11-Jul-2019 16:28:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.9251. 11-Jul-2019 16:28:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-1.575

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:40

Channel:Brightfield set at:11-Jul-2019 16:28:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2001. 11-Jul-2019 16:28:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:40

Channel configuration set to: GFP: 11-Jul-2019 16:28:41

Channel:GFP set at:11-Jul-2019 16:28:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2001. 11-Jul-2019 16:28:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-1.675

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:44

Channel:Brightfield set at:11-Jul-2019 16:28:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8501. 11-Jul-2019 16:28:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:45

Channel configuration set to: GFP: 11-Jul-2019 16:28:46

Channel:GFP set at:11-Jul-2019 16:28:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8501. 11-Jul-2019 16:28:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-1.55

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:49

Channel:Brightfield set at:11-Jul-2019 16:28:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.8001. 11-Jul-2019 16:28:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:50

Channel configuration set to: GFP: 11-Jul-2019 16:28:51

Channel:GFP set at:11-Jul-2019 16:28:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.8001. 11-Jul-2019 16:28:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-0.95

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:54

Channel:Brightfield set at:11-Jul-2019 16:28:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.8251. 11-Jul-2019 16:28:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:54

Channel configuration set to: GFP: 11-Jul-2019 16:28:56

Channel:GFP set at:11-Jul-2019 16:28:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.8251. 11-Jul-2019 16:28:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-2.4

Channel configuration set to: Brightfield: 11-Jul-2019 16:28:59

Channel:Brightfield set at:11-Jul-2019 16:28:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.9751. 11-Jul-2019 16:28:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:28:59

Channel configuration set to: GFP: 11-Jul-2019 16:29:01

Channel:GFP set at:11-Jul-2019 16:29:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.9751. 11-Jul-2019 16:29:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:29:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:1 completed at:11-Jul-2019 16:29:02

Time since start of timelapse:88.7084

PFS is locked

Time to next time point:211.3059



------Time point_2------

Memory available for all arrays: 61013426176

Memory used by Matlab: 2249330688

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-4.375

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:35

Channel:Brightfield set at:11-Jul-2019 16:32:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8751. 11-Jul-2019 16:32:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:32:36

Channel configuration set to: GFP: 11-Jul-2019 16:32:37

Channel:GFP set at:11-Jul-2019 16:32:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.9001. 11-Jul-2019 16:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:32:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-5.15

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:40

Channel:Brightfield set at:11-Jul-2019 16:32:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.7251. 11-Jul-2019 16:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:32:41

Channel configuration set to: GFP: 11-Jul-2019 16:32:42

Channel:GFP set at:11-Jul-2019 16:32:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.7251. 11-Jul-2019 16:32:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:32:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:45

Channel:Brightfield set at:11-Jul-2019 16:32:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.7001. 11-Jul-2019 16:32:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:32:46

Channel configuration set to: GFP: 11-Jul-2019 16:32:47

Channel:GFP set at:11-Jul-2019 16:32:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.7001. 11-Jul-2019 16:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:32:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-3.925

PFS not locked. Waiting for lock...

PFS took 0.050000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:50

Channel:Brightfield set at:11-Jul-2019 16:32:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.3751. 11-Jul-2019 16:32:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:32:50

Channel configuration set to: GFP: 11-Jul-2019 16:32:51

Channel:GFP set at:11-Jul-2019 16:32:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.3751. 11-Jul-2019 16:32:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:32:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-3.075

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:54

Channel:Brightfield set at:11-Jul-2019 16:32:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8001. 11-Jul-2019 16:32:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:32:55

Channel configuration set to: GFP: 11-Jul-2019 16:32:56

Channel:GFP set at:11-Jul-2019 16:32:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8001. 11-Jul-2019 16:32:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:32:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-4.5

Channel configuration set to: Brightfield: 11-Jul-2019 16:32:59

Channel:Brightfield set at:11-Jul-2019 16:32:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2001. 11-Jul-2019 16:32:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:32:59

Channel configuration set to: GFP: 11-Jul-2019 16:33:01

Channel:GFP set at:11-Jul-2019 16:33:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2001. 11-Jul-2019 16:33:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-2.4

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:05

Channel:Brightfield set at:11-Jul-2019 16:33:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5001. 11-Jul-2019 16:33:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:05

Channel configuration set to: GFP: 11-Jul-2019 16:33:07

Channel:GFP set at:11-Jul-2019 16:33:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5001. 11-Jul-2019 16:33:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-3

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:10

Channel:Brightfield set at:11-Jul-2019 16:33:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 16:33:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:11

Channel configuration set to: GFP: 11-Jul-2019 16:33:12

Channel:GFP set at:11-Jul-2019 16:33:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 16:33:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-2.35

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:16

Channel:Brightfield set at:11-Jul-2019 16:33:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1751. 11-Jul-2019 16:33:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:16

Channel configuration set to: GFP: 11-Jul-2019 16:33:18

Channel:GFP set at:11-Jul-2019 16:33:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1501. 11-Jul-2019 16:33:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-2.675

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:21

Channel:Brightfield set at:11-Jul-2019 16:33:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 16:33:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:21

Channel configuration set to: GFP: 11-Jul-2019 16:33:22

Channel:GFP set at:11-Jul-2019 16:33:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 16:33:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-1.875

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:25

Channel:Brightfield set at:11-Jul-2019 16:33:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7751. 11-Jul-2019 16:33:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:25

Channel configuration set to: GFP: 11-Jul-2019 16:33:27

Channel:GFP set at:11-Jul-2019 16:33:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 16:33:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-1.775

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:29

Channel:Brightfield set at:11-Jul-2019 16:33:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 16:33:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:30

Channel configuration set to: GFP: 11-Jul-2019 16:33:31

Channel:GFP set at:11-Jul-2019 16:33:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 16:33:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-1.725

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:35

Channel:Brightfield set at:11-Jul-2019 16:33:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7751. 11-Jul-2019 16:33:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:36

Channel configuration set to: GFP: 11-Jul-2019 16:33:37

Channel:GFP set at:11-Jul-2019 16:33:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7751. 11-Jul-2019 16:33:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-1.875

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:40

Channel:Brightfield set at:11-Jul-2019 16:33:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.0001. 11-Jul-2019 16:33:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:40

Channel configuration set to: GFP: 11-Jul-2019 16:33:41

Channel:GFP set at:11-Jul-2019 16:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.0001. 11-Jul-2019 16:33:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-2.25

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:45

Channel:Brightfield set at:11-Jul-2019 16:33:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.5501. 11-Jul-2019 16:33:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:45

Channel configuration set to: GFP: 11-Jul-2019 16:33:46

Channel:GFP set at:11-Jul-2019 16:33:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.5501. 11-Jul-2019 16:33:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-2.7

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:50

Channel:Brightfield set at:11-Jul-2019 16:33:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.2001. 11-Jul-2019 16:33:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:50

Channel configuration set to: GFP: 11-Jul-2019 16:33:51

Channel:GFP set at:11-Jul-2019 16:33:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.2001. 11-Jul-2019 16:33:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:54

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-2.075

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:55

Channel:Brightfield set at:11-Jul-2019 16:33:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.4751. 11-Jul-2019 16:33:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:55

Channel configuration set to: GFP: 11-Jul-2019 16:33:57

Channel:GFP set at:11-Jul-2019 16:33:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.5001. 11-Jul-2019 16:33:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:33:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:33:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-2.525

Channel configuration set to: Brightfield: 11-Jul-2019 16:34:00

Channel:Brightfield set at:11-Jul-2019 16:34:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8001. 11-Jul-2019 16:34:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:34:00

Channel configuration set to: GFP: 11-Jul-2019 16:34:01

Channel:GFP set at:11-Jul-2019 16:34:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8001. 11-Jul-2019 16:34:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:34:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:2 completed at:11-Jul-2019 16:34:03

Time since start of timelapse:389.8057

PFS is locked

Time to next time point:210.2107



------Time point_3------

Memory available for all arrays: 61039972352

Memory used by Matlab: 2238038016

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-4.45

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:35

Channel:Brightfield set at:11-Jul-2019 16:37:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8501. 11-Jul-2019 16:37:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:37:36

Channel configuration set to: GFP: 11-Jul-2019 16:37:37

Channel:GFP set at:11-Jul-2019 16:37:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8501. 11-Jul-2019 16:37:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:37:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:40

Channel:Brightfield set at:11-Jul-2019 16:37:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.6251. 11-Jul-2019 16:37:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:37:41

Channel configuration set to: GFP: 11-Jul-2019 16:37:42

Channel:GFP set at:11-Jul-2019 16:37:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.6251. 11-Jul-2019 16:37:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:37:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-5.325

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:45

Channel:Brightfield set at:11-Jul-2019 16:37:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.5501. 11-Jul-2019 16:37:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:37:46

Channel configuration set to: GFP: 11-Jul-2019 16:37:47

Channel:GFP set at:11-Jul-2019 16:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.5251. 11-Jul-2019 16:37:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:37:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-4.35

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:50

Channel:Brightfield set at:11-Jul-2019 16:37:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.3001. 11-Jul-2019 16:37:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:37:50

Channel configuration set to: GFP: 11-Jul-2019 16:37:51

Channel:GFP set at:11-Jul-2019 16:37:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.3001. 11-Jul-2019 16:37:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:37:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-2.725

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:54

Channel:Brightfield set at:11-Jul-2019 16:37:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.7751. 11-Jul-2019 16:37:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:37:54

Channel configuration set to: GFP: 11-Jul-2019 16:37:56

Channel:GFP set at:11-Jul-2019 16:37:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.7751. 11-Jul-2019 16:37:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:37:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-4.625

Channel configuration set to: Brightfield: 11-Jul-2019 16:37:59

Channel:Brightfield set at:11-Jul-2019 16:37:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 16:37:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:37:59

Channel configuration set to: GFP: 11-Jul-2019 16:38:00

Channel:GFP set at:11-Jul-2019 16:38:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 16:38:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-2.725

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:04

Channel:Brightfield set at:11-Jul-2019 16:38:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1251. 11-Jul-2019 16:38:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:05

Channel configuration set to: GFP: 11-Jul-2019 16:38:06

Channel:GFP set at:11-Jul-2019 16:38:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1251. 11-Jul-2019 16:38:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-2.175

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:09

Channel:Brightfield set at:11-Jul-2019 16:38:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 16:38:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:09

Channel configuration set to: GFP: 11-Jul-2019 16:38:11

Channel:GFP set at:11-Jul-2019 16:38:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 16:38:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-3.85

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:14

Channel:Brightfield set at:11-Jul-2019 16:38:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 16:38:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:14

Channel configuration set to: GFP: 11-Jul-2019 16:38:15

Channel:GFP set at:11-Jul-2019 16:38:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 16:38:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-2.85

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:19

Channel:Brightfield set at:11-Jul-2019 16:38:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0751. 11-Jul-2019 16:38:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:19

Channel configuration set to: GFP: 11-Jul-2019 16:38:21

Channel:GFP set at:11-Jul-2019 16:38:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0751. 11-Jul-2019 16:38:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-2.1

PFS not locked. Waiting for lock...

PFS took 0.050000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:23

Channel:Brightfield set at:11-Jul-2019 16:38:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 16:38:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:24

Channel configuration set to: GFP: 11-Jul-2019 16:38:25

Channel:GFP set at:11-Jul-2019 16:38:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 16:38:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:25

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:27

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-2.775

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:29

Channel:Brightfield set at:11-Jul-2019 16:38:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 16:38:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:29

Channel configuration set to: GFP: 11-Jul-2019 16:38:30

Channel:GFP set at:11-Jul-2019 16:38:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 16:38:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:32

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-1.85

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:34

Channel:Brightfield set at:11-Jul-2019 16:38:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6751. 11-Jul-2019 16:38:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:34

Channel configuration set to: GFP: 11-Jul-2019 16:38:36

Channel:GFP set at:11-Jul-2019 16:38:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6751. 11-Jul-2019 16:38:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-1.95

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:39

Channel:Brightfield set at:11-Jul-2019 16:38:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.8751. 11-Jul-2019 16:38:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:39

Channel configuration set to: GFP: 11-Jul-2019 16:38:40

Channel:GFP set at:11-Jul-2019 16:38:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.8751. 11-Jul-2019 16:38:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:42

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-1.825

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:43

Channel:Brightfield set at:11-Jul-2019 16:38:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.5501. 11-Jul-2019 16:38:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:44

Channel configuration set to: GFP: 11-Jul-2019 16:38:45

Channel:GFP set at:11-Jul-2019 16:38:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.5501. 11-Jul-2019 16:38:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:45

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-1.85

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:48

Channel:Brightfield set at:11-Jul-2019 16:38:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.5501. 11-Jul-2019 16:38:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:49

Channel configuration set to: GFP: 11-Jul-2019 16:38:50

Channel:GFP set at:11-Jul-2019 16:38:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.5251. 11-Jul-2019 16:38:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:50

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-1.575

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:53

Channel:Brightfield set at:11-Jul-2019 16:38:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.2001. 11-Jul-2019 16:38:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:53

Channel configuration set to: GFP: 11-Jul-2019 16:38:55

Channel:GFP set at:11-Jul-2019 16:38:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.2251. 11-Jul-2019 16:38:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:55

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-1.1

Channel configuration set to: Brightfield: 11-Jul-2019 16:38:57

Channel:Brightfield set at:11-Jul-2019 16:38:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.0751. 11-Jul-2019 16:38:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:58

Channel configuration set to: GFP: 11-Jul-2019 16:38:59

Channel:GFP set at:11-Jul-2019 16:38:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.0501. 11-Jul-2019 16:38:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:38:59

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:3 completed at:11-Jul-2019 16:39:01

Time since start of timelapse:687.4499

PFS is locked

Time to next time point:212.5687



------Time point_4------

Memory available for all arrays: 61053321216

Memory used by Matlab: 2224279552

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:42:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-4.75

Channel configuration set to: Brightfield: 11-Jul-2019 16:42:35

Channel:Brightfield set at:11-Jul-2019 16:42:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.6001. 11-Jul-2019 16:42:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:42:36

Channel configuration set to: GFP: 11-Jul-2019 16:42:37

Channel:GFP set at:11-Jul-2019 16:42:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.6001. 11-Jul-2019 16:42:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:42:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:42:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 16:42:40

Channel:Brightfield set at:11-Jul-2019 16:42:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.4001. 11-Jul-2019 16:42:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:42:41

Channel configuration set to: GFP: 11-Jul-2019 16:42:42

Channel:GFP set at:11-Jul-2019 16:42:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.4001. 11-Jul-2019 16:42:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:42:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:42:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-4.6

Channel configuration set to: Brightfield: 11-Jul-2019 16:42:45

Channel:Brightfield set at:11-Jul-2019 16:42:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.2751. 11-Jul-2019 16:42:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:42:45

Channel configuration set to: GFP: 11-Jul-2019 16:42:46

Channel:GFP set at:11-Jul-2019 16:42:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.3001. 11-Jul-2019 16:42:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:42:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:42:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-4.8

Channel configuration set to: Brightfield: 11-Jul-2019 16:42:50

Channel:Brightfield set at:11-Jul-2019 16:42:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.9001. 11-Jul-2019 16:42:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:42:50

Channel configuration set to: GFP: 11-Jul-2019 16:42:51

Channel:GFP set at:11-Jul-2019 16:42:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.9001. 11-Jul-2019 16:42:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:42:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:42:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-3.5

Channel configuration set to: Brightfield: 11-Jul-2019 16:42:54

Channel:Brightfield set at:11-Jul-2019 16:42:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.3001. 11-Jul-2019 16:42:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:42:55

Channel configuration set to: GFP: 11-Jul-2019 16:42:56

Channel:GFP set at:11-Jul-2019 16:42:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.3001. 11-Jul-2019 16:42:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:42:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:42:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-3.875

PFS not locked. Waiting for lock...

PFS took 0.750000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:00

Channel:Brightfield set at:11-Jul-2019 16:43:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6251. 11-Jul-2019 16:43:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:00

Channel configuration set to: GFP: 11-Jul-2019 16:43:01

Channel:GFP set at:11-Jul-2019 16:43:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6001. 11-Jul-2019 16:43:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-3.15

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:05

Channel:Brightfield set at:11-Jul-2019 16:43:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 16:43:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:05

Channel configuration set to: GFP: 11-Jul-2019 16:43:06

Channel:GFP set at:11-Jul-2019 16:43:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7501. 11-Jul-2019 16:43:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-2.55

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:09

Channel:Brightfield set at:11-Jul-2019 16:43:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3251. 11-Jul-2019 16:43:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:10

Channel configuration set to: GFP: 11-Jul-2019 16:43:11

Channel:GFP set at:11-Jul-2019 16:43:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3501. 11-Jul-2019 16:43:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-3.3

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:14

Channel:Brightfield set at:11-Jul-2019 16:43:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 16:43:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:14

Channel configuration set to: GFP: 11-Jul-2019 16:43:16

Channel:GFP set at:11-Jul-2019 16:43:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 16:43:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-3.225

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:19

Channel:Brightfield set at:11-Jul-2019 16:43:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 16:43:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:20

Channel configuration set to: GFP: 11-Jul-2019 16:43:21

Channel:GFP set at:11-Jul-2019 16:43:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 16:43:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-2.8

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:24

Channel:Brightfield set at:11-Jul-2019 16:43:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 16:43:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:24

Channel configuration set to: GFP: 11-Jul-2019 16:43:26

Channel:GFP set at:11-Jul-2019 16:43:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 16:43:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-3.2

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:29

Channel:Brightfield set at:11-Jul-2019 16:43:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 16:43:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:29

Channel configuration set to: GFP: 11-Jul-2019 16:43:30

Channel:GFP set at:11-Jul-2019 16:43:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 16:43:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:32

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-1.5

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:34

Channel:Brightfield set at:11-Jul-2019 16:43:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2501. 11-Jul-2019 16:43:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:34

Channel configuration set to: GFP: 11-Jul-2019 16:43:36

Channel:GFP set at:11-Jul-2019 16:43:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2751. 11-Jul-2019 16:43:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-2.35

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:39

Channel:Brightfield set at:11-Jul-2019 16:43:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4751. 11-Jul-2019 16:43:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:39

Channel configuration set to: GFP: 11-Jul-2019 16:43:40

Channel:GFP set at:11-Jul-2019 16:43:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4751. 11-Jul-2019 16:43:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:42

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-2.1

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:43

Channel:Brightfield set at:11-Jul-2019 16:43:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2501. 11-Jul-2019 16:43:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:44

Channel configuration set to: GFP: 11-Jul-2019 16:43:45

Channel:GFP set at:11-Jul-2019 16:43:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2501. 11-Jul-2019 16:43:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:45

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-3.175

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:48

Channel:Brightfield set at:11-Jul-2019 16:43:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.6001. 11-Jul-2019 16:43:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:48

Channel configuration set to: GFP: 11-Jul-2019 16:43:49

Channel:GFP set at:11-Jul-2019 16:43:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.6001. 11-Jul-2019 16:43:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:50

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:51

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-1.775

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:53

Channel:Brightfield set at:11-Jul-2019 16:43:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.9751. 11-Jul-2019 16:43:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:53

Channel configuration set to: GFP: 11-Jul-2019 16:43:54

Channel:GFP set at:11-Jul-2019 16:43:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.9751. 11-Jul-2019 16:43:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:55

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:56

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-3

Channel configuration set to: Brightfield: 11-Jul-2019 16:43:58

Channel:Brightfield set at:11-Jul-2019 16:43:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.4001. 11-Jul-2019 16:43:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:43:58

Channel configuration set to: GFP: 11-Jul-2019 16:43:59

Channel:GFP set at:11-Jul-2019 16:43:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.4001. 11-Jul-2019 16:44:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:44:00

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:4 completed at:11-Jul-2019 16:44:01

Time since start of timelapse:987.477

PFS is locked

Time to next time point:212.5449



------Time point_5------

Memory available for all arrays: 61035225088

Memory used by Matlab: 2226167808

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:47:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 16:47:35

Channel:Brightfield set at:11-Jul-2019 16:47:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 16:47:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:47:35

Channel configuration set to: GFP: 11-Jul-2019 16:47:36

Channel:GFP set at:11-Jul-2019 16:47:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0251. 11-Jul-2019 16:47:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:47:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:47:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 16:47:40

Channel:Brightfield set at:11-Jul-2019 16:47:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.0001. 11-Jul-2019 16:47:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:47:40

Channel configuration set to: GFP: 11-Jul-2019 16:47:42

Channel:GFP set at:11-Jul-2019 16:47:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.0001. 11-Jul-2019 16:47:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:47:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:47:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-4.85

Channel configuration set to: Brightfield: 11-Jul-2019 16:47:44

Channel:Brightfield set at:11-Jul-2019 16:47:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.0501. 11-Jul-2019 16:47:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:47:45

Channel configuration set to: GFP: 11-Jul-2019 16:47:46

Channel:GFP set at:11-Jul-2019 16:47:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3388.0501. 11-Jul-2019 16:47:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:47:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:47:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-5.15

Channel configuration set to: Brightfield: 11-Jul-2019 16:47:49

Channel:Brightfield set at:11-Jul-2019 16:47:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.5001. 11-Jul-2019 16:47:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:47:50

Channel configuration set to: GFP: 11-Jul-2019 16:47:51

Channel:GFP set at:11-Jul-2019 16:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.4751. 11-Jul-2019 16:47:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:47:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:47:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-4.65

Channel configuration set to: Brightfield: 11-Jul-2019 16:47:54

Channel:Brightfield set at:11-Jul-2019 16:47:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3001. 11-Jul-2019 16:47:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:47:55

Channel configuration set to: GFP: 11-Jul-2019 16:47:56

Channel:GFP set at:11-Jul-2019 16:47:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2751. 11-Jul-2019 16:47:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:47:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:47:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-4.475

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:00

Channel:Brightfield set at:11-Jul-2019 16:48:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1501. 11-Jul-2019 16:48:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:00

Channel configuration set to: GFP: 11-Jul-2019 16:48:01

Channel:GFP set at:11-Jul-2019 16:48:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1501. 11-Jul-2019 16:48:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-3.35

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:05

Channel:Brightfield set at:11-Jul-2019 16:48:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 11-Jul-2019 16:48:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:05

Channel configuration set to: GFP: 11-Jul-2019 16:48:06

Channel:GFP set at:11-Jul-2019 16:48:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 11-Jul-2019 16:48:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-4

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:10

Channel:Brightfield set at:11-Jul-2019 16:48:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 16:48:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:10

Channel configuration set to: GFP: 11-Jul-2019 16:48:12

Channel:GFP set at:11-Jul-2019 16:48:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 16:48:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-3.35

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:15

Channel:Brightfield set at:11-Jul-2019 16:48:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 11-Jul-2019 16:48:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:16

Channel configuration set to: GFP: 11-Jul-2019 16:48:17

Channel:GFP set at:11-Jul-2019 16:48:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 16:48:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-3.625

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:20

Channel:Brightfield set at:11-Jul-2019 16:48:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 11-Jul-2019 16:48:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:21

Channel configuration set to: GFP: 11-Jul-2019 16:48:22

Channel:GFP set at:11-Jul-2019 16:48:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 11-Jul-2019 16:48:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-2.825

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:25

Channel:Brightfield set at:11-Jul-2019 16:48:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 11-Jul-2019 16:48:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:25

Channel configuration set to: GFP: 11-Jul-2019 16:48:26

Channel:GFP set at:11-Jul-2019 16:48:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 16:48:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-3.575

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:30

Channel:Brightfield set at:11-Jul-2019 16:48:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 16:48:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:30

Channel configuration set to: GFP: 11-Jul-2019 16:48:31

Channel:GFP set at:11-Jul-2019 16:48:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 16:48:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-2.75

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:35

Channel:Brightfield set at:11-Jul-2019 16:48:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 16:48:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:36

Channel configuration set to: GFP: 11-Jul-2019 16:48:37

Channel:GFP set at:11-Jul-2019 16:48:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8251. 11-Jul-2019 16:48:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-2.9

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:40

Channel:Brightfield set at:11-Jul-2019 16:48:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 16:48:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:40

Channel configuration set to: GFP: 11-Jul-2019 16:48:42

Channel:GFP set at:11-Jul-2019 16:48:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8751. 11-Jul-2019 16:48:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-2.375

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:45

Channel:Brightfield set at:11-Jul-2019 16:48:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.9501. 11-Jul-2019 16:48:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:45

Channel configuration set to: GFP: 11-Jul-2019 16:48:46

Channel:GFP set at:11-Jul-2019 16:48:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.9751. 11-Jul-2019 16:48:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-2.425

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:50

Channel:Brightfield set at:11-Jul-2019 16:48:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.9251. 11-Jul-2019 16:48:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:50

Channel configuration set to: GFP: 11-Jul-2019 16:48:51

Channel:GFP set at:11-Jul-2019 16:48:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.9501. 11-Jul-2019 16:48:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-2.025

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:54

Channel:Brightfield set at:11-Jul-2019 16:48:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.7251. 11-Jul-2019 16:48:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:55

Channel configuration set to: GFP: 11-Jul-2019 16:48:56

Channel:GFP set at:11-Jul-2019 16:48:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.7251. 11-Jul-2019 16:48:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:48:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-3.325

Channel configuration set to: Brightfield: 11-Jul-2019 16:48:59

Channel:Brightfield set at:11-Jul-2019 16:48:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.0251. 11-Jul-2019 16:49:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:49:00

Channel configuration set to: GFP: 11-Jul-2019 16:49:01

Channel:GFP set at:11-Jul-2019 16:49:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.0001. 11-Jul-2019 16:49:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:49:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:5 completed at:11-Jul-2019 16:49:03

Time since start of timelapse:1289.7345

PFS is locked

Time to next time point:210.2892



------Time point_6------

Memory available for all arrays: 61044752384

Memory used by Matlab: 2224562176

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:52:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 11-Jul-2019 16:52:36

Channel:Brightfield set at:11-Jul-2019 16:52:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.0001. 11-Jul-2019 16:52:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:52:36

Channel configuration set to: GFP: 11-Jul-2019 16:52:37

Channel:GFP set at:11-Jul-2019 16:52:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.9751. 11-Jul-2019 16:52:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:52:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:52:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 11-Jul-2019 16:52:40

Channel:Brightfield set at:11-Jul-2019 16:52:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8251. 11-Jul-2019 16:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:52:41

Channel configuration set to: GFP: 11-Jul-2019 16:52:42

Channel:GFP set at:11-Jul-2019 16:52:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.8251. 11-Jul-2019 16:52:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:52:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:52:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-6.05

PFS not locked. Waiting for lock...

PFS took 0.800000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 16:52:46

Channel:Brightfield set at:11-Jul-2019 16:52:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.7001. 11-Jul-2019 16:52:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:52:46

Channel configuration set to: GFP: 11-Jul-2019 16:52:47

Channel:GFP set at:11-Jul-2019 16:52:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.7001. 11-Jul-2019 16:52:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:52:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:52:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 11-Jul-2019 16:52:50

Channel:Brightfield set at:11-Jul-2019 16:52:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2751. 11-Jul-2019 16:52:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:52:51

Channel configuration set to: GFP: 11-Jul-2019 16:52:52

Channel:GFP set at:11-Jul-2019 16:52:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2751. 11-Jul-2019 16:52:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:52:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:52:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-5.025

Channel configuration set to: Brightfield: 11-Jul-2019 16:52:55

Channel:Brightfield set at:11-Jul-2019 16:52:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 16:52:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:52:56

Channel configuration set to: GFP: 11-Jul-2019 16:52:57

Channel:GFP set at:11-Jul-2019 16:52:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 16:52:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:52:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:52:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-4.8

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:00

Channel:Brightfield set at:11-Jul-2019 16:53:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 16:53:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:00

Channel configuration set to: GFP: 11-Jul-2019 16:53:01

Channel:GFP set at:11-Jul-2019 16:53:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 16:53:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-3.625

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:05

Channel:Brightfield set at:11-Jul-2019 16:53:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 16:53:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:06

Channel configuration set to: GFP: 11-Jul-2019 16:53:07

Channel:GFP set at:11-Jul-2019 16:53:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 16:53:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-4.275

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:10

Channel:Brightfield set at:11-Jul-2019 16:53:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6751. 11-Jul-2019 16:53:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:11

Channel configuration set to: GFP: 11-Jul-2019 16:53:12

Channel:GFP set at:11-Jul-2019 16:53:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 16:53:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-3.6

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:16

Channel:Brightfield set at:11-Jul-2019 16:53:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 16:53:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:16

Channel configuration set to: GFP: 11-Jul-2019 16:53:17

Channel:GFP set at:11-Jul-2019 16:53:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 16:53:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-3.95

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:21

Channel:Brightfield set at:11-Jul-2019 16:53:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 11-Jul-2019 16:53:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:21

Channel configuration set to: GFP: 11-Jul-2019 16:53:22

Channel:GFP set at:11-Jul-2019 16:53:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 16:53:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-3.975

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:25

Channel:Brightfield set at:11-Jul-2019 16:53:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 16:53:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:25

Channel configuration set to: GFP: 11-Jul-2019 16:53:27

Channel:GFP set at:11-Jul-2019 16:53:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 16:53:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-4.45

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:30

Channel:Brightfield set at:11-Jul-2019 16:53:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 16:53:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:30

Channel configuration set to: GFP: 11-Jul-2019 16:53:31

Channel:GFP set at:11-Jul-2019 16:53:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 16:53:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-3.175

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:35

Channel:Brightfield set at:11-Jul-2019 16:53:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 16:53:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:36

Channel configuration set to: GFP: 11-Jul-2019 16:53:37

Channel:GFP set at:11-Jul-2019 16:53:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 16:53:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-3.125

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:40

Channel:Brightfield set at:11-Jul-2019 16:53:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 16:53:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:40

Channel configuration set to: GFP: 11-Jul-2019 16:53:41

Channel:GFP set at:11-Jul-2019 16:53:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 16:53:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-2.7

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:44

Channel:Brightfield set at:11-Jul-2019 16:53:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6751. 11-Jul-2019 16:53:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:45

Channel configuration set to: GFP: 11-Jul-2019 16:53:46

Channel:GFP set at:11-Jul-2019 16:53:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6751. 11-Jul-2019 16:53:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-2.725

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:49

Channel:Brightfield set at:11-Jul-2019 16:53:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.7001. 11-Jul-2019 16:53:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:50

Channel configuration set to: GFP: 11-Jul-2019 16:53:51

Channel:GFP set at:11-Jul-2019 16:53:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.7001. 11-Jul-2019 16:53:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-2.3

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:54

Channel:Brightfield set at:11-Jul-2019 16:53:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.4501. 11-Jul-2019 16:53:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:54

Channel configuration set to: GFP: 11-Jul-2019 16:53:56

Channel:GFP set at:11-Jul-2019 16:53:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.4501. 11-Jul-2019 16:53:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-1.9

Channel configuration set to: Brightfield: 11-Jul-2019 16:53:58

Channel:Brightfield set at:11-Jul-2019 16:53:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.2251. 11-Jul-2019 16:53:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:53:59

Channel configuration set to: GFP: 11-Jul-2019 16:54:00

Channel:GFP set at:11-Jul-2019 16:54:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.2001. 11-Jul-2019 16:54:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:54:00

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:6 completed at:11-Jul-2019 16:54:02

Time since start of timelapse:1588.6269

PFS is locked

Time to next time point:211.3984



------Time point_7------

Memory available for all arrays: 61043503104

Memory used by Matlab: 2224058368

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:35

Channel:Brightfield set at:11-Jul-2019 16:57:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.8001. 11-Jul-2019 16:57:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:57:36

Channel configuration set to: GFP: 11-Jul-2019 16:57:37

Channel:GFP set at:11-Jul-2019 16:57:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.8001. 11-Jul-2019 16:57:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:57:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:40

Channel:Brightfield set at:11-Jul-2019 16:57:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.6251. 11-Jul-2019 16:57:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:57:41

Channel configuration set to: GFP: 11-Jul-2019 16:57:42

Channel:GFP set at:11-Jul-2019 16:57:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.6251. 11-Jul-2019 16:57:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:57:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-5.525

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:45

Channel:Brightfield set at:11-Jul-2019 16:57:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.5501. 11-Jul-2019 16:57:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:57:45

Channel configuration set to: GFP: 11-Jul-2019 16:57:46

Channel:GFP set at:11-Jul-2019 16:57:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.5501. 11-Jul-2019 16:57:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:57:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:50

Channel:Brightfield set at:11-Jul-2019 16:57:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2501. 11-Jul-2019 16:57:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:57:50

Channel configuration set to: GFP: 11-Jul-2019 16:57:51

Channel:GFP set at:11-Jul-2019 16:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2501. 11-Jul-2019 16:57:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:57:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:55

Channel:Brightfield set at:11-Jul-2019 16:57:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 11-Jul-2019 16:57:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:57:55

Channel configuration set to: GFP: 11-Jul-2019 16:57:56

Channel:GFP set at:11-Jul-2019 16:57:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 11-Jul-2019 16:57:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:57:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-5.1

Channel configuration set to: Brightfield: 11-Jul-2019 16:57:59

Channel:Brightfield set at:11-Jul-2019 16:57:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 16:57:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:57:59

Channel configuration set to: GFP: 11-Jul-2019 16:58:01

Channel:GFP set at:11-Jul-2019 16:58:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6251. 11-Jul-2019 16:58:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-3.9

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:04

Channel:Brightfield set at:11-Jul-2019 16:58:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 16:58:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:05

Channel configuration set to: GFP: 11-Jul-2019 16:58:06

Channel:GFP set at:11-Jul-2019 16:58:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 16:58:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-4.625

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:09

Channel:Brightfield set at:11-Jul-2019 16:58:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 11-Jul-2019 16:58:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:10

Channel configuration set to: GFP: 11-Jul-2019 16:58:11

Channel:GFP set at:11-Jul-2019 16:58:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 11-Jul-2019 16:58:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-3.95

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:15

Channel:Brightfield set at:11-Jul-2019 16:58:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 16:58:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:15

Channel configuration set to: GFP: 11-Jul-2019 16:58:17

Channel:GFP set at:11-Jul-2019 16:58:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 16:58:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-4.3

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:20

Channel:Brightfield set at:11-Jul-2019 16:58:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 11-Jul-2019 16:58:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:20

Channel configuration set to: GFP: 11-Jul-2019 16:58:21

Channel:GFP set at:11-Jul-2019 16:58:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 16:58:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-4.1

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:24

Channel:Brightfield set at:11-Jul-2019 16:58:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 16:58:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:25

Channel configuration set to: GFP: 11-Jul-2019 16:58:26

Channel:GFP set at:11-Jul-2019 16:58:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 11-Jul-2019 16:58:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-4.675

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:29

Channel:Brightfield set at:11-Jul-2019 16:58:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 11-Jul-2019 16:58:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:29

Channel configuration set to: GFP: 11-Jul-2019 16:58:30

Channel:GFP set at:11-Jul-2019 16:58:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 11-Jul-2019 16:58:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:34

Channel:Brightfield set at:11-Jul-2019 16:58:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 16:58:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:34

Channel configuration set to: GFP: 11-Jul-2019 16:58:35

Channel:GFP set at:11-Jul-2019 16:58:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 16:58:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:37

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-3.35

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:38

Channel:Brightfield set at:11-Jul-2019 16:58:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 16:58:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:39

Channel configuration set to: GFP: 11-Jul-2019 16:58:40

Channel:GFP set at:11-Jul-2019 16:58:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5001. 11-Jul-2019 16:58:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:40

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:42

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-3.9

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:43

Channel:Brightfield set at:11-Jul-2019 16:58:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2251. 11-Jul-2019 16:58:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:43

Channel configuration set to: GFP: 11-Jul-2019 16:58:44

Channel:GFP set at:11-Jul-2019 16:58:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2251. 11-Jul-2019 16:58:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:45

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:46

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-2.95

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:48

Channel:Brightfield set at:11-Jul-2019 16:58:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.4501. 11-Jul-2019 16:58:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:48

Channel configuration set to: GFP: 11-Jul-2019 16:58:49

Channel:GFP set at:11-Jul-2019 16:58:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.4501. 11-Jul-2019 16:58:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:50

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:51

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-2.625

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:52

Channel:Brightfield set at:11-Jul-2019 16:58:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.1251. 11-Jul-2019 16:58:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:53

Channel configuration set to: GFP: 11-Jul-2019 16:58:54

Channel:GFP set at:11-Jul-2019 16:58:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.1251. 11-Jul-2019 16:58:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:54

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:56

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-3.875

Channel configuration set to: Brightfield: 11-Jul-2019 16:58:57

Channel:Brightfield set at:11-Jul-2019 16:58:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5251. 11-Jul-2019 16:58:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:58

Channel configuration set to: GFP: 11-Jul-2019 16:58:59

Channel:GFP set at:11-Jul-2019 16:58:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5251. 11-Jul-2019 16:58:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 16:58:59

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:7 completed at:11-Jul-2019 16:59:01

Time since start of timelapse:1887.2762

PFS is locked

Time to next time point:212.7506



------Time point_8------

Memory available for all arrays: 61082742784

Memory used by Matlab: 2185252864

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:35

Channel:Brightfield set at:11-Jul-2019 17:02:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3751. 11-Jul-2019 17:02:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:02:36

Channel configuration set to: GFP: 11-Jul-2019 17:02:37

Channel:GFP set at:11-Jul-2019 17:02:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3751. 11-Jul-2019 17:02:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:02:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:40

Channel:Brightfield set at:11-Jul-2019 17:02:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2001. 11-Jul-2019 17:02:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:02:40

Channel configuration set to: GFP: 11-Jul-2019 17:02:42

Channel:GFP set at:11-Jul-2019 17:02:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2001. 11-Jul-2019 17:02:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:02:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:45

Channel:Brightfield set at:11-Jul-2019 17:02:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.1501. 11-Jul-2019 17:02:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:02:46

Channel configuration set to: GFP: 11-Jul-2019 17:02:47

Channel:GFP set at:11-Jul-2019 17:02:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.1501. 11-Jul-2019 17:02:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:02:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:50

Channel:Brightfield set at:11-Jul-2019 17:02:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.8251. 11-Jul-2019 17:02:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:02:51

Channel configuration set to: GFP: 11-Jul-2019 17:02:52

Channel:GFP set at:11-Jul-2019 17:02:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.8251. 11-Jul-2019 17:02:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:02:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:55

Channel:Brightfield set at:11-Jul-2019 17:02:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 17:02:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:02:55

Channel configuration set to: GFP: 11-Jul-2019 17:02:57

Channel:GFP set at:11-Jul-2019 17:02:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1751. 11-Jul-2019 17:02:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:02:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-5.525

Channel configuration set to: Brightfield: 11-Jul-2019 17:02:59

Channel:Brightfield set at:11-Jul-2019 17:02:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0751. 11-Jul-2019 17:03:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:00

Channel configuration set to: GFP: 11-Jul-2019 17:03:01

Channel:GFP set at:11-Jul-2019 17:03:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0501. 11-Jul-2019 17:03:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-4.425

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:05

Channel:Brightfield set at:11-Jul-2019 17:03:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 17:03:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:05

Channel configuration set to: GFP: 11-Jul-2019 17:03:07

Channel:GFP set at:11-Jul-2019 17:03:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 17:03:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:10

Channel:Brightfield set at:11-Jul-2019 17:03:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 17:03:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:11

Channel configuration set to: GFP: 11-Jul-2019 17:03:12

Channel:GFP set at:11-Jul-2019 17:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 17:03:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:15

Channel:Brightfield set at:11-Jul-2019 17:03:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 17:03:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:15

Channel configuration set to: GFP: 11-Jul-2019 17:03:16

Channel:GFP set at:11-Jul-2019 17:03:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 17:03:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-4.75

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:20

Channel:Brightfield set at:11-Jul-2019 17:03:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 17:03:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:20

Channel configuration set to: GFP: 11-Jul-2019 17:03:22

Channel:GFP set at:11-Jul-2019 17:03:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 17:03:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-4.475

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:24

Channel:Brightfield set at:11-Jul-2019 17:03:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 17:03:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:25

Channel configuration set to: GFP: 11-Jul-2019 17:03:26

Channel:GFP set at:11-Jul-2019 17:03:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 17:03:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-4.45

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:29

Channel:Brightfield set at:11-Jul-2019 17:03:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 17:03:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:30

Channel configuration set to: GFP: 11-Jul-2019 17:03:31

Channel:GFP set at:11-Jul-2019 17:03:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 17:03:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-3.825

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:35

Channel:Brightfield set at:11-Jul-2019 17:03:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7251. 11-Jul-2019 17:03:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:35

Channel configuration set to: GFP: 11-Jul-2019 17:03:36

Channel:GFP set at:11-Jul-2019 17:03:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 17:03:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-3.775

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:39

Channel:Brightfield set at:11-Jul-2019 17:03:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 17:03:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:40

Channel configuration set to: GFP: 11-Jul-2019 17:03:41

Channel:GFP set at:11-Jul-2019 17:03:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 17:03:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-4.275

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:44

Channel:Brightfield set at:11-Jul-2019 17:03:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 11-Jul-2019 17:03:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:44

Channel configuration set to: GFP: 11-Jul-2019 17:03:45

Channel:GFP set at:11-Jul-2019 17:03:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 17:03:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-3.25

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:49

Channel:Brightfield set at:11-Jul-2019 17:03:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.1751. 11-Jul-2019 17:03:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:49

Channel configuration set to: GFP: 11-Jul-2019 17:03:51

Channel:GFP set at:11-Jul-2019 17:03:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.1751. 11-Jul-2019 17:03:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-3.05

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:54

Channel:Brightfield set at:11-Jul-2019 17:03:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.7001. 11-Jul-2019 17:03:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:54

Channel configuration set to: GFP: 11-Jul-2019 17:03:56

Channel:GFP set at:11-Jul-2019 17:03:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.7001. 11-Jul-2019 17:03:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-4.2

Channel configuration set to: Brightfield: 11-Jul-2019 17:03:59

Channel:Brightfield set at:11-Jul-2019 17:03:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1751. 11-Jul-2019 17:03:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:03:59

Channel configuration set to: GFP: 11-Jul-2019 17:04:01

Channel:GFP set at:11-Jul-2019 17:04:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1501. 11-Jul-2019 17:04:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:04:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:8 completed at:11-Jul-2019 17:04:02

Time since start of timelapse:2188.7788

PFS is locked

Time to next time point:211.2494



------Time point_9------

Memory available for all arrays: 61051211776

Memory used by Matlab: 2199097344

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:35

Channel:Brightfield set at:11-Jul-2019 17:07:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1001. 11-Jul-2019 17:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:07:36

Channel configuration set to: GFP: 11-Jul-2019 17:07:37

Channel:GFP set at:11-Jul-2019 17:07:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0751. 11-Jul-2019 17:07:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:07:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-7.8

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:40

Channel:Brightfield set at:11-Jul-2019 17:07:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3001. 11-Jul-2019 17:07:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:07:40

Channel configuration set to: GFP: 11-Jul-2019 17:07:41

Channel:GFP set at:11-Jul-2019 17:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3251. 11-Jul-2019 17:07:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:07:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:44

Channel:Brightfield set at:11-Jul-2019 17:07:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.0501. 11-Jul-2019 17:07:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:07:45

Channel configuration set to: GFP: 11-Jul-2019 17:07:46

Channel:GFP set at:11-Jul-2019 17:07:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3387.0501. 11-Jul-2019 17:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:07:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:49

Channel:Brightfield set at:11-Jul-2019 17:07:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5751. 11-Jul-2019 17:07:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:07:50

Channel configuration set to: GFP: 11-Jul-2019 17:07:51

Channel:GFP set at:11-Jul-2019 17:07:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5751. 11-Jul-2019 17:07:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:07:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:54

Channel:Brightfield set at:11-Jul-2019 17:07:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 17:07:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:07:55

Channel configuration set to: GFP: 11-Jul-2019 17:07:56

Channel:GFP set at:11-Jul-2019 17:07:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 17:07:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:07:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 17:07:59

Channel:Brightfield set at:11-Jul-2019 17:07:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 17:07:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:07:59

Channel configuration set to: GFP: 11-Jul-2019 17:08:00

Channel:GFP set at:11-Jul-2019 17:08:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 17:08:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-4.675

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:04

Channel:Brightfield set at:11-Jul-2019 17:08:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 11-Jul-2019 17:08:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:04

Channel configuration set to: GFP: 11-Jul-2019 17:08:06

Channel:GFP set at:11-Jul-2019 17:08:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 11-Jul-2019 17:08:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:09

Channel:Brightfield set at:11-Jul-2019 17:08:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 17:08:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:10

Channel configuration set to: GFP: 11-Jul-2019 17:08:11

Channel:GFP set at:11-Jul-2019 17:08:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 17:08:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:14

Channel:Brightfield set at:11-Jul-2019 17:08:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 17:08:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:14

Channel configuration set to: GFP: 11-Jul-2019 17:08:15

Channel:GFP set at:11-Jul-2019 17:08:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 17:08:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-4.95

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:19

Channel:Brightfield set at:11-Jul-2019 17:08:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 17:08:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:19

Channel configuration set to: GFP: 11-Jul-2019 17:08:20

Channel:GFP set at:11-Jul-2019 17:08:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 17:08:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:22

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-4.6

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:23

Channel:Brightfield set at:11-Jul-2019 17:08:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 17:08:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:24

Channel configuration set to: GFP: 11-Jul-2019 17:08:25

Channel:GFP set at:11-Jul-2019 17:08:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 17:08:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:25

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:27

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-4.625

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:28

Channel:Brightfield set at:11-Jul-2019 17:08:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 17:08:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:29

Channel configuration set to: GFP: 11-Jul-2019 17:08:30

Channel:GFP set at:11-Jul-2019 17:08:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 17:08:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:30

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:32

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.125

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:34

Channel:Brightfield set at:11-Jul-2019 17:08:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 17:08:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:34

Channel configuration set to: GFP: 11-Jul-2019 17:08:35

Channel:GFP set at:11-Jul-2019 17:08:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 17:08:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:37

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.075

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:38

Channel:Brightfield set at:11-Jul-2019 17:08:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 17:08:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:39

Channel configuration set to: GFP: 11-Jul-2019 17:08:40

Channel:GFP set at:11-Jul-2019 17:08:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 17:08:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:40

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:42

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-4.575

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:43

Channel:Brightfield set at:11-Jul-2019 17:08:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 11-Jul-2019 17:08:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:43

Channel configuration set to: GFP: 11-Jul-2019 17:08:44

Channel:GFP set at:11-Jul-2019 17:08:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 11-Jul-2019 17:08:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:45

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:46

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-3.5

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:48

Channel:Brightfield set at:11-Jul-2019 17:08:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.9251. 11-Jul-2019 17:08:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:48

Channel configuration set to: GFP: 11-Jul-2019 17:08:49

Channel:GFP set at:11-Jul-2019 17:08:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.9251. 11-Jul-2019 17:08:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:50

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:51

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-3.225

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:52

Channel:Brightfield set at:11-Jul-2019 17:08:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.5501. 11-Jul-2019 17:08:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:53

Channel configuration set to: GFP: 11-Jul-2019 17:08:54

Channel:GFP set at:11-Jul-2019 17:08:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.5751. 11-Jul-2019 17:08:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:54

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:56

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-2.775

Channel configuration set to: Brightfield: 11-Jul-2019 17:08:57

Channel:Brightfield set at:11-Jul-2019 17:08:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.4751. 11-Jul-2019 17:08:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:57

Channel configuration set to: GFP: 11-Jul-2019 17:08:58

Channel:GFP set at:11-Jul-2019 17:08:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.4751. 11-Jul-2019 17:08:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:08:59

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:9 completed at:11-Jul-2019 17:09:00

Time since start of timelapse:2486.9115

PFS is locked

Time to next time point:213.1183



------Time point_10------

Memory available for all arrays: 61062963200

Memory used by Matlab: 2199097344

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:35

Channel:Brightfield set at:11-Jul-2019 17:12:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 17:12:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:12:36

Channel configuration set to: GFP: 11-Jul-2019 17:12:37

Channel:GFP set at:11-Jul-2019 17:12:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8251. 11-Jul-2019 17:12:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:12:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:40

Channel:Brightfield set at:11-Jul-2019 17:12:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7251. 11-Jul-2019 17:12:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:12:41

Channel configuration set to: GFP: 11-Jul-2019 17:12:42

Channel:GFP set at:11-Jul-2019 17:12:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7251. 11-Jul-2019 17:12:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:12:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:45

Channel:Brightfield set at:11-Jul-2019 17:12:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 17:12:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:12:46

Channel configuration set to: GFP: 11-Jul-2019 17:12:47

Channel:GFP set at:11-Jul-2019 17:12:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 17:12:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:12:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:50

Channel:Brightfield set at:11-Jul-2019 17:12:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2001. 11-Jul-2019 17:12:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:12:50

Channel configuration set to: GFP: 11-Jul-2019 17:12:51

Channel:GFP set at:11-Jul-2019 17:12:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1751. 11-Jul-2019 17:12:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:12:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:55

Channel:Brightfield set at:11-Jul-2019 17:12:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7751. 11-Jul-2019 17:12:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:12:55

Channel configuration set to: GFP: 11-Jul-2019 17:12:57

Channel:GFP set at:11-Jul-2019 17:12:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 17:12:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:12:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 17:12:59

Channel:Brightfield set at:11-Jul-2019 17:12:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 17:13:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:00

Channel configuration set to: GFP: 11-Jul-2019 17:13:01

Channel:GFP set at:11-Jul-2019 17:13:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 17:13:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-4.775

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:04

Channel:Brightfield set at:11-Jul-2019 17:13:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1251. 11-Jul-2019 17:13:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:05

Channel configuration set to: GFP: 11-Jul-2019 17:13:06

Channel:GFP set at:11-Jul-2019 17:13:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 17:13:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-4.225

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:09

Channel:Brightfield set at:11-Jul-2019 17:13:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 17:13:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:09

Channel configuration set to: GFP: 11-Jul-2019 17:13:11

Channel:GFP set at:11-Jul-2019 17:13:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 17:13:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.125

PFS not locked. Waiting for lock...

PFS took 0.750000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:14

Channel:Brightfield set at:11-Jul-2019 17:13:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 11-Jul-2019 17:13:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:15

Channel configuration set to: GFP: 11-Jul-2019 17:13:16

Channel:GFP set at:11-Jul-2019 17:13:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 17:13:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.125

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:19

Channel:Brightfield set at:11-Jul-2019 17:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 17:13:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:19

Channel configuration set to: GFP: 11-Jul-2019 17:13:21

Channel:GFP set at:11-Jul-2019 17:13:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 17:13:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-4.8

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:24

Channel:Brightfield set at:11-Jul-2019 17:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 17:13:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:24

Channel configuration set to: GFP: 11-Jul-2019 17:13:25

Channel:GFP set at:11-Jul-2019 17:13:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 17:13:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:27

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-4.775

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:29

Channel:Brightfield set at:11-Jul-2019 17:13:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 17:13:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:30

Channel configuration set to: GFP: 11-Jul-2019 17:13:31

Channel:GFP set at:11-Jul-2019 17:13:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 17:13:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.225

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:34

Channel:Brightfield set at:11-Jul-2019 17:13:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3251. 11-Jul-2019 17:13:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:35

Channel configuration set to: GFP: 11-Jul-2019 17:13:36

Channel:GFP set at:11-Jul-2019 17:13:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 11-Jul-2019 17:13:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.175

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:39

Channel:Brightfield set at:11-Jul-2019 17:13:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6751. 11-Jul-2019 17:13:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:40

Channel configuration set to: GFP: 11-Jul-2019 17:13:41

Channel:GFP set at:11-Jul-2019 17:13:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 17:13:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-3.95

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:44

Channel:Brightfield set at:11-Jul-2019 17:13:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 17:13:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:45

Channel configuration set to: GFP: 11-Jul-2019 17:13:46

Channel:GFP set at:11-Jul-2019 17:13:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 17:13:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-3.675

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:49

Channel:Brightfield set at:11-Jul-2019 17:13:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 17:13:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:50

Channel configuration set to: GFP: 11-Jul-2019 17:13:51

Channel:GFP set at:11-Jul-2019 17:13:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 17:13:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.025

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:54

Channel:Brightfield set at:11-Jul-2019 17:13:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.1751. 11-Jul-2019 17:13:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:54

Channel configuration set to: GFP: 11-Jul-2019 17:13:55

Channel:GFP set at:11-Jul-2019 17:13:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.1751. 11-Jul-2019 17:13:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-4.7

Channel configuration set to: Brightfield: 11-Jul-2019 17:13:59

Channel:Brightfield set at:11-Jul-2019 17:13:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 17:13:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:13:59

Channel configuration set to: GFP: 11-Jul-2019 17:14:00

Channel:GFP set at:11-Jul-2019 17:14:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 11-Jul-2019 17:14:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:14:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:10 completed at:11-Jul-2019 17:14:02

Time since start of timelapse:2788.8589

PFS is locked

Time to next time point:211.1726



------Time point_11------

Memory available for all arrays: 61067280384

Memory used by Matlab: 2199097344

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:35

Channel:Brightfield set at:11-Jul-2019 17:17:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 17:17:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:17:36

Channel configuration set to: GFP: 11-Jul-2019 17:17:37

Channel:GFP set at:11-Jul-2019 17:17:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5001. 11-Jul-2019 17:17:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:17:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:40

Channel:Brightfield set at:11-Jul-2019 17:17:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4251. 11-Jul-2019 17:17:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:17:40

Channel configuration set to: GFP: 11-Jul-2019 17:17:42

Channel:GFP set at:11-Jul-2019 17:17:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4751. 11-Jul-2019 17:17:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:17:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:44

Channel:Brightfield set at:11-Jul-2019 17:17:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.6251. 11-Jul-2019 17:17:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:17:45

Channel configuration set to: GFP: 11-Jul-2019 17:17:46

Channel:GFP set at:11-Jul-2019 17:17:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.6251. 11-Jul-2019 17:17:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:17:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:49

Channel:Brightfield set at:11-Jul-2019 17:17:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 17:17:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:17:50

Channel configuration set to: GFP: 11-Jul-2019 17:17:51

Channel:GFP set at:11-Jul-2019 17:17:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 17:17:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:17:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:54

Channel:Brightfield set at:11-Jul-2019 17:17:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 17:17:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:17:54

Channel configuration set to: GFP: 11-Jul-2019 17:17:55

Channel:GFP set at:11-Jul-2019 17:17:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 17:17:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:17:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 11-Jul-2019 17:17:58

Channel:Brightfield set at:11-Jul-2019 17:17:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 17:17:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:17:59

Channel configuration set to: GFP: 11-Jul-2019 17:18:00

Channel:GFP set at:11-Jul-2019 17:18:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 17:18:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:00

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:02

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.025

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:04

Channel:Brightfield set at:11-Jul-2019 17:18:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 17:18:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:04

Channel configuration set to: GFP: 11-Jul-2019 17:18:05

Channel:GFP set at:11-Jul-2019 17:18:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 17:18:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.025

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:08

Channel:Brightfield set at:11-Jul-2019 17:18:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 11-Jul-2019 17:18:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:09

Channel configuration set to: GFP: 11-Jul-2019 17:18:10

Channel:GFP set at:11-Jul-2019 17:18:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 11-Jul-2019 17:18:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:10

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:12

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:13

Channel:Brightfield set at:11-Jul-2019 17:18:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 17:18:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:13

Channel configuration set to: GFP: 11-Jul-2019 17:18:14

Channel:GFP set at:11-Jul-2019 17:18:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 11-Jul-2019 17:18:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:15

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:17

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:18

Channel:Brightfield set at:11-Jul-2019 17:18:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 17:18:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:18

Channel configuration set to: GFP: 11-Jul-2019 17:18:20

Channel:GFP set at:11-Jul-2019 17:18:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 17:18:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:20

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:22

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-4.65

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:22

Channel:Brightfield set at:11-Jul-2019 17:18:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 11-Jul-2019 17:18:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:23

Channel configuration set to: GFP: 11-Jul-2019 17:18:24

Channel:GFP set at:11-Jul-2019 17:18:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 11-Jul-2019 17:18:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:24

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:26

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.075

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:27

Channel:Brightfield set at:11-Jul-2019 17:18:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 11-Jul-2019 17:18:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:28

Channel configuration set to: GFP: 11-Jul-2019 17:18:29

Channel:GFP set at:11-Jul-2019 17:18:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 11-Jul-2019 17:18:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:30

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:31

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.6

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:33

Channel:Brightfield set at:11-Jul-2019 17:18:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 17:18:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:33

Channel configuration set to: GFP: 11-Jul-2019 17:18:34

Channel:GFP set at:11-Jul-2019 17:18:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 11-Jul-2019 17:18:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:35

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:36

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.775

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:37

Channel:Brightfield set at:11-Jul-2019 17:18:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1251. 11-Jul-2019 17:18:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:38

Channel configuration set to: GFP: 11-Jul-2019 17:18:39

Channel:GFP set at:11-Jul-2019 17:18:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 17:18:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:39

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:41

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-4.825

PFS not locked. Waiting for lock...

PFS took 0.250000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:42

Channel:Brightfield set at:11-Jul-2019 17:18:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 11-Jul-2019 17:18:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:42

Channel configuration set to: GFP: 11-Jul-2019 17:18:44

Channel:GFP set at:11-Jul-2019 17:18:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 11-Jul-2019 17:18:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:44

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:46

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.05

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:47

Channel:Brightfield set at:11-Jul-2019 17:18:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3751. 11-Jul-2019 17:18:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:47

Channel configuration set to: GFP: 11-Jul-2019 17:18:49

Channel:GFP set at:11-Jul-2019 17:18:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3751. 11-Jul-2019 17:18:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:49

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:50

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.35

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:51

Channel:Brightfield set at:11-Jul-2019 17:18:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 17:18:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:52

Channel configuration set to: GFP: 11-Jul-2019 17:18:53

Channel:GFP set at:11-Jul-2019 17:18:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 17:18:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:53

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:55

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.05

Channel configuration set to: Brightfield: 11-Jul-2019 17:18:57

Channel:Brightfield set at:11-Jul-2019 17:18:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 17:18:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:57

Channel configuration set to: GFP: 11-Jul-2019 17:18:58

Channel:GFP set at:11-Jul-2019 17:18:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 11-Jul-2019 17:18:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:18:59

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:11 completed at:11-Jul-2019 17:19:00

Time since start of timelapse:3086.754

PFS is locked

Time to next time point:213.2791



------Time point_12------

Memory available for all arrays: 61067354112

Memory used by Matlab: 2199097344

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:22:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 17:22:35

Channel:Brightfield set at:11-Jul-2019 17:22:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 17:22:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:22:36

Channel configuration set to: GFP: 11-Jul-2019 17:22:37

Channel:GFP set at:11-Jul-2019 17:22:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 17:22:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:22:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:22:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-7.525

Channel configuration set to: Brightfield: 11-Jul-2019 17:22:41

Channel:Brightfield set at:11-Jul-2019 17:22:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3251. 11-Jul-2019 17:22:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:22:41

Channel configuration set to: GFP: 11-Jul-2019 17:22:42

Channel:GFP set at:11-Jul-2019 17:22:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3251. 11-Jul-2019 17:22:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:22:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:22:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.625

Channel configuration set to: Brightfield: 11-Jul-2019 17:22:46

Channel:Brightfield set at:11-Jul-2019 17:22:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2501. 11-Jul-2019 17:22:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:22:46

Channel configuration set to: GFP: 11-Jul-2019 17:22:47

Channel:GFP set at:11-Jul-2019 17:22:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2251. 11-Jul-2019 17:22:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:22:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:22:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 17:22:51

Channel:Brightfield set at:11-Jul-2019 17:22:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 17:22:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:22:51

Channel configuration set to: GFP: 11-Jul-2019 17:22:52

Channel:GFP set at:11-Jul-2019 17:22:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 17:22:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:22:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:22:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 17:22:55

Channel:Brightfield set at:11-Jul-2019 17:22:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 17:22:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:22:56

Channel configuration set to: GFP: 11-Jul-2019 17:22:57

Channel:GFP set at:11-Jul-2019 17:22:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 17:22:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:22:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:22:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:00

Channel:Brightfield set at:11-Jul-2019 17:23:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 11-Jul-2019 17:23:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:00

Channel configuration set to: GFP: 11-Jul-2019 17:23:01

Channel:GFP set at:11-Jul-2019 17:23:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 11-Jul-2019 17:23:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.15

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:05

Channel:Brightfield set at:11-Jul-2019 17:23:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 17:23:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:05

Channel configuration set to: GFP: 11-Jul-2019 17:23:06

Channel:GFP set at:11-Jul-2019 17:23:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 17:23:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:10

Channel:Brightfield set at:11-Jul-2019 17:23:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 17:23:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:10

Channel configuration set to: GFP: 11-Jul-2019 17:23:12

Channel:GFP set at:11-Jul-2019 17:23:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 17:23:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.175

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:15

Channel:Brightfield set at:11-Jul-2019 17:23:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 17:23:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:16

Channel configuration set to: GFP: 11-Jul-2019 17:23:17

Channel:GFP set at:11-Jul-2019 17:23:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 17:23:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:20

Channel:Brightfield set at:11-Jul-2019 17:23:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 17:23:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:20

Channel configuration set to: GFP: 11-Jul-2019 17:23:22

Channel:GFP set at:11-Jul-2019 17:23:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 17:23:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:25

Channel:Brightfield set at:11-Jul-2019 17:23:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 11-Jul-2019 17:23:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:25

Channel configuration set to: GFP: 11-Jul-2019 17:23:26

Channel:GFP set at:11-Jul-2019 17:23:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 17:23:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-4.85

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:29

Channel:Brightfield set at:11-Jul-2019 17:23:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 17:23:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:30

Channel configuration set to: GFP: 11-Jul-2019 17:23:31

Channel:GFP set at:11-Jul-2019 17:23:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 11-Jul-2019 17:23:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.55

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:35

Channel:Brightfield set at:11-Jul-2019 17:23:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 17:23:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:35

Channel configuration set to: GFP: 11-Jul-2019 17:23:36

Channel:GFP set at:11-Jul-2019 17:23:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 17:23:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.425

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:39

Channel:Brightfield set at:11-Jul-2019 17:23:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 17:23:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:40

Channel configuration set to: GFP: 11-Jul-2019 17:23:41

Channel:GFP set at:11-Jul-2019 17:23:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 17:23:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-4.975

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:44

Channel:Brightfield set at:11-Jul-2019 17:23:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 17:23:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:44

Channel configuration set to: GFP: 11-Jul-2019 17:23:45

Channel:GFP set at:11-Jul-2019 17:23:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 17:23:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:48

Channel:Brightfield set at:11-Jul-2019 17:23:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 11-Jul-2019 17:23:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:49

Channel configuration set to: GFP: 11-Jul-2019 17:23:50

Channel:GFP set at:11-Jul-2019 17:23:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 11-Jul-2019 17:23:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:50

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-3.75

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:53

Channel:Brightfield set at:11-Jul-2019 17:23:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.0001. 11-Jul-2019 17:23:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:54

Channel configuration set to: GFP: 11-Jul-2019 17:23:55

Channel:GFP set at:11-Jul-2019 17:23:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.0001. 11-Jul-2019 17:23:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.15

Channel configuration set to: Brightfield: 11-Jul-2019 17:23:59

Channel:Brightfield set at:11-Jul-2019 17:23:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 17:23:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:23:59

Channel configuration set to: GFP: 11-Jul-2019 17:24:00

Channel:GFP set at:11-Jul-2019 17:24:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 17:24:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:24:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:12 completed at:11-Jul-2019 17:24:02

Time since start of timelapse:3388.8044

PFS is locked

Time to next time point:211.2299



------Time point_13------

Memory available for all arrays: 61066518528

Memory used by Matlab: 2199097344

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:27:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 17:27:35

Channel:Brightfield set at:11-Jul-2019 17:27:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 17:27:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:27:36

Channel configuration set to: GFP: 11-Jul-2019 17:27:37

Channel:GFP set at:11-Jul-2019 17:27:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 11-Jul-2019 17:27:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:27:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:27:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 11-Jul-2019 17:27:40

Channel:Brightfield set at:11-Jul-2019 17:27:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1251. 11-Jul-2019 17:27:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:27:41

Channel configuration set to: GFP: 11-Jul-2019 17:27:42

Channel:GFP set at:11-Jul-2019 17:27:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1251. 11-Jul-2019 17:27:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:27:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:27:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.85

Channel configuration set to: Brightfield: 11-Jul-2019 17:27:45

Channel:Brightfield set at:11-Jul-2019 17:27:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 17:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:27:46

Channel configuration set to: GFP: 11-Jul-2019 17:27:47

Channel:GFP set at:11-Jul-2019 17:27:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 17:27:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:27:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:27:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 11-Jul-2019 17:27:50

Channel:Brightfield set at:11-Jul-2019 17:27:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 11-Jul-2019 17:27:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:27:50

Channel configuration set to: GFP: 11-Jul-2019 17:27:52

Channel:GFP set at:11-Jul-2019 17:27:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5501. 11-Jul-2019 17:27:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:27:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:27:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 11-Jul-2019 17:27:55

Channel:Brightfield set at:11-Jul-2019 17:27:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 11-Jul-2019 17:27:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:27:55

Channel configuration set to: GFP: 11-Jul-2019 17:27:57

Channel:GFP set at:11-Jul-2019 17:27:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 11-Jul-2019 17:27:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:27:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:27:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:00

Channel:Brightfield set at:11-Jul-2019 17:28:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 17:28:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:01

Channel configuration set to: GFP: 11-Jul-2019 17:28:02

Channel:GFP set at:11-Jul-2019 17:28:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9501. 11-Jul-2019 17:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:05

Channel:Brightfield set at:11-Jul-2019 17:28:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 11-Jul-2019 17:28:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:05

Channel configuration set to: GFP: 11-Jul-2019 17:28:06

Channel:GFP set at:11-Jul-2019 17:28:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 11-Jul-2019 17:28:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:10

Channel:Brightfield set at:11-Jul-2019 17:28:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 17:28:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:10

Channel configuration set to: GFP: 11-Jul-2019 17:28:11

Channel:GFP set at:11-Jul-2019 17:28:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 17:28:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:15

Channel:Brightfield set at:11-Jul-2019 17:28:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 17:28:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:15

Channel configuration set to: GFP: 11-Jul-2019 17:28:17

Channel:GFP set at:11-Jul-2019 17:28:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 17:28:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:20

Channel:Brightfield set at:11-Jul-2019 17:28:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 17:28:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:20

Channel configuration set to: GFP: 11-Jul-2019 17:28:22

Channel:GFP set at:11-Jul-2019 17:28:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 17:28:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.475

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:25

Channel:Brightfield set at:11-Jul-2019 17:28:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 17:28:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:25

Channel configuration set to: GFP: 11-Jul-2019 17:28:26

Channel:GFP set at:11-Jul-2019 17:28:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 17:28:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:30

Channel:Brightfield set at:11-Jul-2019 17:28:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1251. 11-Jul-2019 17:28:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:30

Channel configuration set to: GFP: 11-Jul-2019 17:28:31

Channel:GFP set at:11-Jul-2019 17:28:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 17:28:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.325

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:34

Channel:Brightfield set at:11-Jul-2019 17:28:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 17:28:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:35

Channel configuration set to: GFP: 11-Jul-2019 17:28:36

Channel:GFP set at:11-Jul-2019 17:28:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 17:28:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.7

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:39

Channel:Brightfield set at:11-Jul-2019 17:28:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 17:28:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:40

Channel configuration set to: GFP: 11-Jul-2019 17:28:41

Channel:GFP set at:11-Jul-2019 17:28:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 17:28:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-4.625

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:44

Channel:Brightfield set at:11-Jul-2019 17:28:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 11-Jul-2019 17:28:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:44

Channel configuration set to: GFP: 11-Jul-2019 17:28:46

Channel:GFP set at:11-Jul-2019 17:28:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 11-Jul-2019 17:28:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.15

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:49

Channel:Brightfield set at:11-Jul-2019 17:28:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2001. 11-Jul-2019 17:28:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:49

Channel configuration set to: GFP: 11-Jul-2019 17:28:51

Channel:GFP set at:11-Jul-2019 17:28:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1751. 11-Jul-2019 17:28:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.075

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:54

Channel:Brightfield set at:11-Jul-2019 17:28:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7001. 11-Jul-2019 17:28:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:54

Channel configuration set to: GFP: 11-Jul-2019 17:28:55

Channel:GFP set at:11-Jul-2019 17:28:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7001. 11-Jul-2019 17:28:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 11-Jul-2019 17:28:59

Channel:Brightfield set at:11-Jul-2019 17:28:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 11-Jul-2019 17:28:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:28:59

Channel configuration set to: GFP: 11-Jul-2019 17:29:00

Channel:GFP set at:11-Jul-2019 17:29:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 11-Jul-2019 17:29:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:29:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:13 completed at:11-Jul-2019 17:29:02

Time since start of timelapse:3689.0031

PFS is locked

Time to next time point:211.0326



------Time point_14------

Memory available for all arrays: 61061955584

Memory used by Matlab: 2199621632

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:32:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 11-Jul-2019 17:32:35

Channel:Brightfield set at:11-Jul-2019 17:32:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8751. 11-Jul-2019 17:32:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:32:36

Channel configuration set to: GFP: 11-Jul-2019 17:32:37

Channel:GFP set at:11-Jul-2019 17:32:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 17:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:32:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:32:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 11-Jul-2019 17:32:40

Channel:Brightfield set at:11-Jul-2019 17:32:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 17:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:32:41

Channel configuration set to: GFP: 11-Jul-2019 17:32:42

Channel:GFP set at:11-Jul-2019 17:32:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 17:32:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:32:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:32:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 11-Jul-2019 17:32:45

Channel:Brightfield set at:11-Jul-2019 17:32:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.1751. 11-Jul-2019 17:32:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:32:45

Channel configuration set to: GFP: 11-Jul-2019 17:32:47

Channel:GFP set at:11-Jul-2019 17:32:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2001. 11-Jul-2019 17:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:32:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:32:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.275

Channel configuration set to: Brightfield: 11-Jul-2019 17:32:50

Channel:Brightfield set at:11-Jul-2019 17:32:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 11-Jul-2019 17:32:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:32:51

Channel configuration set to: GFP: 11-Jul-2019 17:32:52

Channel:GFP set at:11-Jul-2019 17:32:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 11-Jul-2019 17:32:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:32:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:32:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 17:32:55

Channel:Brightfield set at:11-Jul-2019 17:32:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9501. 11-Jul-2019 17:32:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:32:55

Channel configuration set to: GFP: 11-Jul-2019 17:32:57

Channel:GFP set at:11-Jul-2019 17:32:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 17:32:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:32:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:32:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:00

Channel:Brightfield set at:11-Jul-2019 17:33:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 17:33:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:00

Channel configuration set to: GFP: 11-Jul-2019 17:33:02

Channel:GFP set at:11-Jul-2019 17:33:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 17:33:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:05

Channel:Brightfield set at:11-Jul-2019 17:33:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 17:33:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:05

Channel configuration set to: GFP: 11-Jul-2019 17:33:06

Channel:GFP set at:11-Jul-2019 17:33:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 11-Jul-2019 17:33:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.175

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:09

Channel:Brightfield set at:11-Jul-2019 17:33:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 11-Jul-2019 17:33:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:10

Channel configuration set to: GFP: 11-Jul-2019 17:33:11

Channel:GFP set at:11-Jul-2019 17:33:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 11-Jul-2019 17:33:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:15

Channel:Brightfield set at:11-Jul-2019 17:33:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 17:33:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:15

Channel configuration set to: GFP: 11-Jul-2019 17:33:16

Channel:GFP set at:11-Jul-2019 17:33:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 17:33:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:20

Channel:Brightfield set at:11-Jul-2019 17:33:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 11-Jul-2019 17:33:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:20

Channel configuration set to: GFP: 11-Jul-2019 17:33:21

Channel:GFP set at:11-Jul-2019 17:33:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 11-Jul-2019 17:33:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:24

Channel:Brightfield set at:11-Jul-2019 17:33:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 17:33:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:25

Channel configuration set to: GFP: 11-Jul-2019 17:33:26

Channel:GFP set at:11-Jul-2019 17:33:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 17:33:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.475

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:29

Channel:Brightfield set at:11-Jul-2019 17:33:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 17:33:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:30

Channel configuration set to: GFP: 11-Jul-2019 17:33:31

Channel:GFP set at:11-Jul-2019 17:33:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 17:33:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.025

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:35

Channel:Brightfield set at:11-Jul-2019 17:33:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 17:33:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:35

Channel configuration set to: GFP: 11-Jul-2019 17:33:36

Channel:GFP set at:11-Jul-2019 17:33:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 11-Jul-2019 17:33:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.9

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:39

Channel:Brightfield set at:11-Jul-2019 17:33:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 17:33:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:40

Channel configuration set to: GFP: 11-Jul-2019 17:33:41

Channel:GFP set at:11-Jul-2019 17:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 17:33:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.525

PFS not locked. Waiting for lock...

PFS took 0.150000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:44

Channel:Brightfield set at:11-Jul-2019 17:33:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 17:33:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:44

Channel configuration set to: GFP: 11-Jul-2019 17:33:46

Channel:GFP set at:11-Jul-2019 17:33:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 17:33:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.45

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:49

Channel:Brightfield set at:11-Jul-2019 17:33:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 17:33:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:49

Channel configuration set to: GFP: 11-Jul-2019 17:33:51

Channel:GFP set at:11-Jul-2019 17:33:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 17:33:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.925

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:53

Channel:Brightfield set at:11-Jul-2019 17:33:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 17:33:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:54

Channel configuration set to: GFP: 11-Jul-2019 17:33:55

Channel:GFP set at:11-Jul-2019 17:33:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 17:33:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:55

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.6

Channel configuration set to: Brightfield: 11-Jul-2019 17:33:59

Channel:Brightfield set at:11-Jul-2019 17:33:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 17:33:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:33:59

Channel configuration set to: GFP: 11-Jul-2019 17:34:00

Channel:GFP set at:11-Jul-2019 17:34:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7251. 11-Jul-2019 17:34:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:34:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:14 completed at:11-Jul-2019 17:34:02

Time since start of timelapse:3988.548

PFS is locked

Time to next time point:211.4891



------Time point_15------

Memory available for all arrays: 61077266432

Memory used by Matlab: 2199097344

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:37:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 11-Jul-2019 17:37:35

Channel:Brightfield set at:11-Jul-2019 17:37:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 17:37:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:37:36

Channel configuration set to: GFP: 11-Jul-2019 17:37:37

Channel:GFP set at:11-Jul-2019 17:37:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 17:37:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:37:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:37:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 11-Jul-2019 17:37:40

Channel:Brightfield set at:11-Jul-2019 17:37:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 17:37:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:37:41

Channel configuration set to: GFP: 11-Jul-2019 17:37:42

Channel:GFP set at:11-Jul-2019 17:37:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 17:37:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:37:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:37:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.225

Channel configuration set to: Brightfield: 11-Jul-2019 17:37:45

Channel:Brightfield set at:11-Jul-2019 17:37:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 11-Jul-2019 17:37:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:37:46

Channel configuration set to: GFP: 11-Jul-2019 17:37:47

Channel:GFP set at:11-Jul-2019 17:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 11-Jul-2019 17:37:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:37:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:37:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 11-Jul-2019 17:37:50

Channel:Brightfield set at:11-Jul-2019 17:37:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 11-Jul-2019 17:37:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:37:51

Channel configuration set to: GFP: 11-Jul-2019 17:37:52

Channel:GFP set at:11-Jul-2019 17:37:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 17:37:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:37:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:37:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 17:37:56

Channel:Brightfield set at:11-Jul-2019 17:37:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 17:37:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:37:56

Channel configuration set to: GFP: 11-Jul-2019 17:37:57

Channel:GFP set at:11-Jul-2019 17:37:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9501. 11-Jul-2019 17:37:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:37:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:37:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:01

Channel:Brightfield set at:11-Jul-2019 17:38:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 17:38:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:01

Channel configuration set to: GFP: 11-Jul-2019 17:38:02

Channel:GFP set at:11-Jul-2019 17:38:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 11-Jul-2019 17:38:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.7

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:05

Channel:Brightfield set at:11-Jul-2019 17:38:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 17:38:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:06

Channel configuration set to: GFP: 11-Jul-2019 17:38:07

Channel:GFP set at:11-Jul-2019 17:38:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 17:38:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.15

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:10

Channel:Brightfield set at:11-Jul-2019 17:38:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 11-Jul-2019 17:38:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:10

Channel configuration set to: GFP: 11-Jul-2019 17:38:12

Channel:GFP set at:11-Jul-2019 17:38:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6751. 11-Jul-2019 17:38:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:15

Channel:Brightfield set at:11-Jul-2019 17:38:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 17:38:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:16

Channel configuration set to: GFP: 11-Jul-2019 17:38:17

Channel:GFP set at:11-Jul-2019 17:38:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 17:38:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:20

Channel:Brightfield set at:11-Jul-2019 17:38:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 11-Jul-2019 17:38:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:20

Channel configuration set to: GFP: 11-Jul-2019 17:38:22

Channel:GFP set at:11-Jul-2019 17:38:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 17:38:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:25

Channel:Brightfield set at:11-Jul-2019 17:38:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 17:38:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:25

Channel configuration set to: GFP: 11-Jul-2019 17:38:26

Channel:GFP set at:11-Jul-2019 17:38:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 17:38:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.225

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:29

Channel:Brightfield set at:11-Jul-2019 17:38:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 11-Jul-2019 17:38:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:30

Channel configuration set to: GFP: 11-Jul-2019 17:38:31

Channel:GFP set at:11-Jul-2019 17:38:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 11-Jul-2019 17:38:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.875

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:35

Channel:Brightfield set at:11-Jul-2019 17:38:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6751. 11-Jul-2019 17:38:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:35

Channel configuration set to: GFP: 11-Jul-2019 17:38:37

Channel:GFP set at:11-Jul-2019 17:38:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6751. 11-Jul-2019 17:38:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.775

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:40

Channel:Brightfield set at:11-Jul-2019 17:38:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 17:38:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:40

Channel configuration set to: GFP: 11-Jul-2019 17:38:42

Channel:GFP set at:11-Jul-2019 17:38:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 17:38:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.275

PFS not locked. Waiting for lock...

PFS took 0.200000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:44

Channel:Brightfield set at:11-Jul-2019 17:38:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 17:38:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:45

Channel configuration set to: GFP: 11-Jul-2019 17:38:46

Channel:GFP set at:11-Jul-2019 17:38:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 17:38:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.3

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:50

Channel:Brightfield set at:11-Jul-2019 17:38:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1001. 11-Jul-2019 17:38:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:50

Channel configuration set to: GFP: 11-Jul-2019 17:38:51

Channel:GFP set at:11-Jul-2019 17:38:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1001. 11-Jul-2019 17:38:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-3.975

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:54

Channel:Brightfield set at:11-Jul-2019 17:38:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7751. 11-Jul-2019 17:38:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:55

Channel configuration set to: GFP: 11-Jul-2019 17:38:56

Channel:GFP set at:11-Jul-2019 17:38:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 17:38:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:38:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:38:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 11-Jul-2019 17:39:00

Channel:Brightfield set at:11-Jul-2019 17:39:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 17:39:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:39:00

Channel configuration set to: GFP: 11-Jul-2019 17:39:01

Channel:GFP set at:11-Jul-2019 17:39:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 17:39:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:39:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:15 completed at:11-Jul-2019 17:39:03

Time since start of timelapse:4289.7866

PFS is locked

Time to next time point:210.2517



------Time point_16------

Memory available for all arrays: 61076426752

Memory used by Matlab: 2199097344

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:42:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 11-Jul-2019 17:42:35

Channel:Brightfield set at:11-Jul-2019 17:42:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 17:42:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:42:36

Channel configuration set to: GFP: 11-Jul-2019 17:42:37

Channel:GFP set at:11-Jul-2019 17:42:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 17:42:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:42:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:42:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.975

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 17:42:40

Channel:Brightfield set at:11-Jul-2019 17:42:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 17:42:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:42:41

Channel configuration set to: GFP: 11-Jul-2019 17:42:42

Channel:GFP set at:11-Jul-2019 17:42:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 17:42:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:42:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:42:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 17:42:45

Channel:Brightfield set at:11-Jul-2019 17:42:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.1751. 11-Jul-2019 17:42:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:42:45

Channel configuration set to: GFP: 11-Jul-2019 17:42:46

Channel:GFP set at:11-Jul-2019 17:42:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.1751. 11-Jul-2019 17:42:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:42:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:42:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 11-Jul-2019 17:42:49

Channel:Brightfield set at:11-Jul-2019 17:42:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 11-Jul-2019 17:42:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:42:50

Channel configuration set to: GFP: 11-Jul-2019 17:42:51

Channel:GFP set at:11-Jul-2019 17:42:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 11-Jul-2019 17:42:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:42:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:42:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 17:42:54

Channel:Brightfield set at:11-Jul-2019 17:42:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 17:42:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:42:55

Channel configuration set to: GFP: 11-Jul-2019 17:42:56

Channel:GFP set at:11-Jul-2019 17:42:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 17:42:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:42:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:42:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:00

Channel:Brightfield set at:11-Jul-2019 17:43:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 11-Jul-2019 17:43:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:00

Channel configuration set to: GFP: 11-Jul-2019 17:43:01

Channel:GFP set at:11-Jul-2019 17:43:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 11-Jul-2019 17:43:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:04

Channel:Brightfield set at:11-Jul-2019 17:43:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 17:43:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:05

Channel configuration set to: GFP: 11-Jul-2019 17:43:06

Channel:GFP set at:11-Jul-2019 17:43:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 17:43:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:10

Channel:Brightfield set at:11-Jul-2019 17:43:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 17:43:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:10

Channel configuration set to: GFP: 11-Jul-2019 17:43:11

Channel:GFP set at:11-Jul-2019 17:43:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 17:43:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:15

Channel:Brightfield set at:11-Jul-2019 17:43:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 17:43:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:15

Channel configuration set to: GFP: 11-Jul-2019 17:43:16

Channel:GFP set at:11-Jul-2019 17:43:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 17:43:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:20

Channel:Brightfield set at:11-Jul-2019 17:43:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 17:43:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:20

Channel configuration set to: GFP: 11-Jul-2019 17:43:21

Channel:GFP set at:11-Jul-2019 17:43:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 17:43:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:24

Channel:Brightfield set at:11-Jul-2019 17:43:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 17:43:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:25

Channel configuration set to: GFP: 11-Jul-2019 17:43:26

Channel:GFP set at:11-Jul-2019 17:43:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 17:43:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:29

Channel:Brightfield set at:11-Jul-2019 17:43:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 11-Jul-2019 17:43:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:30

Channel configuration set to: GFP: 11-Jul-2019 17:43:31

Channel:GFP set at:11-Jul-2019 17:43:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 11-Jul-2019 17:43:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.95

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:35

Channel:Brightfield set at:11-Jul-2019 17:43:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 17:43:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:35

Channel configuration set to: GFP: 11-Jul-2019 17:43:37

Channel:GFP set at:11-Jul-2019 17:43:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 17:43:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.775

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:40

Channel:Brightfield set at:11-Jul-2019 17:43:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 17:43:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:40

Channel configuration set to: GFP: 11-Jul-2019 17:43:42

Channel:GFP set at:11-Jul-2019 17:43:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0501. 11-Jul-2019 17:43:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-4.725

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:45

Channel:Brightfield set at:11-Jul-2019 17:43:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 17:43:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:45

Channel configuration set to: GFP: 11-Jul-2019 17:43:46

Channel:GFP set at:11-Jul-2019 17:43:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 17:43:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.375

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:50

Channel:Brightfield set at:11-Jul-2019 17:43:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0251. 11-Jul-2019 17:43:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:50

Channel configuration set to: GFP: 11-Jul-2019 17:43:51

Channel:GFP set at:11-Jul-2019 17:43:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 17:43:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.075

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:54

Channel:Brightfield set at:11-Jul-2019 17:43:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7001. 11-Jul-2019 17:43:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:55

Channel configuration set to: GFP: 11-Jul-2019 17:43:56

Channel:GFP set at:11-Jul-2019 17:43:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7001. 11-Jul-2019 17:43:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:43:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:43:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 17:44:00

Channel:Brightfield set at:11-Jul-2019 17:44:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 17:44:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:44:00

Channel configuration set to: GFP: 11-Jul-2019 17:44:01

Channel:GFP set at:11-Jul-2019 17:44:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 17:44:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:44:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:16 completed at:11-Jul-2019 17:44:03

Time since start of timelapse:4589.7261

PFS is locked

Time to next time point:210.3137



------Time point_17------

Memory available for all arrays: 61066985472

Memory used by Matlab: 2199097344

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:35

Channel:Brightfield set at:11-Jul-2019 17:47:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 17:47:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:47:36

Channel configuration set to: GFP: 11-Jul-2019 17:47:37

Channel:GFP set at:11-Jul-2019 17:47:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 17:47:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:47:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:40

Channel:Brightfield set at:11-Jul-2019 17:47:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 17:47:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:47:41

Channel configuration set to: GFP: 11-Jul-2019 17:47:42

Channel:GFP set at:11-Jul-2019 17:47:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 17:47:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:47:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:45

Channel:Brightfield set at:11-Jul-2019 17:47:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.1501. 11-Jul-2019 17:47:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:47:45

Channel configuration set to: GFP: 11-Jul-2019 17:47:46

Channel:GFP set at:11-Jul-2019 17:47:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.1501. 11-Jul-2019 17:47:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:47:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:50

Channel:Brightfield set at:11-Jul-2019 17:47:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5501. 11-Jul-2019 17:47:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:47:50

Channel configuration set to: GFP: 11-Jul-2019 17:47:51

Channel:GFP set at:11-Jul-2019 17:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 17:47:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:47:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:55

Channel:Brightfield set at:11-Jul-2019 17:47:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 17:47:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:47:55

Channel configuration set to: GFP: 11-Jul-2019 17:47:56

Channel:GFP set at:11-Jul-2019 17:47:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 17:47:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:47:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 11-Jul-2019 17:47:59

Channel:Brightfield set at:11-Jul-2019 17:47:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 17:47:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:47:59

Channel configuration set to: GFP: 11-Jul-2019 17:48:01

Channel:GFP set at:11-Jul-2019 17:48:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 17:48:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:05

Channel:Brightfield set at:11-Jul-2019 17:48:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 17:48:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:05

Channel configuration set to: GFP: 11-Jul-2019 17:48:06

Channel:GFP set at:11-Jul-2019 17:48:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 17:48:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:09

Channel:Brightfield set at:11-Jul-2019 17:48:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 17:48:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:10

Channel configuration set to: GFP: 11-Jul-2019 17:48:11

Channel:GFP set at:11-Jul-2019 17:48:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 17:48:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:15

Channel:Brightfield set at:11-Jul-2019 17:48:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 17:48:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:15

Channel configuration set to: GFP: 11-Jul-2019 17:48:16

Channel:GFP set at:11-Jul-2019 17:48:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 17:48:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:19

Channel:Brightfield set at:11-Jul-2019 17:48:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 11-Jul-2019 17:48:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:20

Channel configuration set to: GFP: 11-Jul-2019 17:48:21

Channel:GFP set at:11-Jul-2019 17:48:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 11-Jul-2019 17:48:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:24

Channel:Brightfield set at:11-Jul-2019 17:48:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 17:48:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:25

Channel configuration set to: GFP: 11-Jul-2019 17:48:26

Channel:GFP set at:11-Jul-2019 17:48:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 17:48:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:29

Channel:Brightfield set at:11-Jul-2019 17:48:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1251. 11-Jul-2019 17:48:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:30

Channel configuration set to: GFP: 11-Jul-2019 17:48:31

Channel:GFP set at:11-Jul-2019 17:48:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 17:48:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.975

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:34

Channel:Brightfield set at:11-Jul-2019 17:48:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 17:48:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:35

Channel configuration set to: GFP: 11-Jul-2019 17:48:36

Channel:GFP set at:11-Jul-2019 17:48:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 17:48:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.875

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:39

Channel:Brightfield set at:11-Jul-2019 17:48:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 17:48:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:40

Channel configuration set to: GFP: 11-Jul-2019 17:48:41

Channel:GFP set at:11-Jul-2019 17:48:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9501. 11-Jul-2019 17:48:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:44

Channel:Brightfield set at:11-Jul-2019 17:48:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4751. 11-Jul-2019 17:48:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:44

Channel configuration set to: GFP: 11-Jul-2019 17:48:45

Channel:GFP set at:11-Jul-2019 17:48:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 17:48:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.325

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:49

Channel:Brightfield set at:11-Jul-2019 17:48:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 17:48:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:49

Channel configuration set to: GFP: 11-Jul-2019 17:48:50

Channel:GFP set at:11-Jul-2019 17:48:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 17:48:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.15

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:54

Channel:Brightfield set at:11-Jul-2019 17:48:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6751. 11-Jul-2019 17:48:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:54

Channel configuration set to: GFP: 11-Jul-2019 17:48:55

Channel:GFP set at:11-Jul-2019 17:48:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6501. 11-Jul-2019 17:48:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 17:48:59

Channel:Brightfield set at:11-Jul-2019 17:48:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 17:48:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:48:59

Channel configuration set to: GFP: 11-Jul-2019 17:49:00

Channel:GFP set at:11-Jul-2019 17:49:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 17:49:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:49:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:17 completed at:11-Jul-2019 17:49:03

Time since start of timelapse:4889.3308

PFS is locked

Time to next time point:210.7106



------Time point_18------

Memory available for all arrays: 61071388672

Memory used by Matlab: 2199101440

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:52:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 11-Jul-2019 17:52:35

Channel:Brightfield set at:11-Jul-2019 17:52:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 17:52:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:52:36

Channel configuration set to: GFP: 11-Jul-2019 17:52:37

Channel:GFP set at:11-Jul-2019 17:52:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 11-Jul-2019 17:52:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:52:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:52:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-7.85

Channel configuration set to: Brightfield: 11-Jul-2019 17:52:40

Channel:Brightfield set at:11-Jul-2019 17:52:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 11-Jul-2019 17:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:52:41

Channel configuration set to: GFP: 11-Jul-2019 17:52:42

Channel:GFP set at:11-Jul-2019 17:52:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 11-Jul-2019 17:52:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:52:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:52:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 11-Jul-2019 17:52:45

Channel:Brightfield set at:11-Jul-2019 17:52:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 17:52:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:52:46

Channel configuration set to: GFP: 11-Jul-2019 17:52:47

Channel:GFP set at:11-Jul-2019 17:52:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 17:52:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:52:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:52:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 17:52:50

Channel:Brightfield set at:11-Jul-2019 17:52:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5501. 11-Jul-2019 17:52:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:52:51

Channel configuration set to: GFP: 11-Jul-2019 17:52:52

Channel:GFP set at:11-Jul-2019 17:52:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 11-Jul-2019 17:52:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:52:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:52:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 17:52:55

Channel:Brightfield set at:11-Jul-2019 17:52:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 17:52:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:52:56

Channel configuration set to: GFP: 11-Jul-2019 17:52:57

Channel:GFP set at:11-Jul-2019 17:52:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0751. 11-Jul-2019 17:52:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:52:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:52:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-6.55

PFS not locked. Waiting for lock...

PFS took 0.700000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:00

Channel:Brightfield set at:11-Jul-2019 17:53:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 11-Jul-2019 17:53:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:01

Channel configuration set to: GFP: 11-Jul-2019 17:53:02

Channel:GFP set at:11-Jul-2019 17:53:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 17:53:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.475

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:05

Channel:Brightfield set at:11-Jul-2019 17:53:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 17:53:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:06

Channel configuration set to: GFP: 11-Jul-2019 17:53:07

Channel:GFP set at:11-Jul-2019 17:53:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 17:53:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:10

Channel:Brightfield set at:11-Jul-2019 17:53:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 17:53:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:11

Channel configuration set to: GFP: 11-Jul-2019 17:53:12

Channel:GFP set at:11-Jul-2019 17:53:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 17:53:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:15

Channel:Brightfield set at:11-Jul-2019 17:53:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 11-Jul-2019 17:53:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:15

Channel configuration set to: GFP: 11-Jul-2019 17:53:16

Channel:GFP set at:11-Jul-2019 17:53:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 17:53:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:20

Channel:Brightfield set at:11-Jul-2019 17:53:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 17:53:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:20

Channel configuration set to: GFP: 11-Jul-2019 17:53:21

Channel:GFP set at:11-Jul-2019 17:53:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 17:53:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.475

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:24

Channel:Brightfield set at:11-Jul-2019 17:53:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 17:53:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:25

Channel configuration set to: GFP: 11-Jul-2019 17:53:26

Channel:GFP set at:11-Jul-2019 17:53:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 17:53:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:29

Channel:Brightfield set at:11-Jul-2019 17:53:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 17:53:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:29

Channel configuration set to: GFP: 11-Jul-2019 17:53:30

Channel:GFP set at:11-Jul-2019 17:53:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 17:53:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.8

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:34

Channel:Brightfield set at:11-Jul-2019 17:53:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 17:53:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:35

Channel configuration set to: GFP: 11-Jul-2019 17:53:36

Channel:GFP set at:11-Jul-2019 17:53:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 11-Jul-2019 17:53:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.8

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:39

Channel:Brightfield set at:11-Jul-2019 17:53:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 17:53:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:40

Channel configuration set to: GFP: 11-Jul-2019 17:53:41

Channel:GFP set at:11-Jul-2019 17:53:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 17:53:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-4.925

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:44

Channel:Brightfield set at:11-Jul-2019 17:53:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 17:53:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:44

Channel configuration set to: GFP: 11-Jul-2019 17:53:45

Channel:GFP set at:11-Jul-2019 17:53:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 17:53:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.2

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:49

Channel:Brightfield set at:11-Jul-2019 17:53:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2251. 11-Jul-2019 17:53:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:50

Channel configuration set to: GFP: 11-Jul-2019 17:53:51

Channel:GFP set at:11-Jul-2019 17:53:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2251. 11-Jul-2019 17:53:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-3.975

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:54

Channel:Brightfield set at:11-Jul-2019 17:53:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 17:53:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:54

Channel configuration set to: GFP: 11-Jul-2019 17:53:56

Channel:GFP set at:11-Jul-2019 17:53:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7751. 11-Jul-2019 17:53:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 11-Jul-2019 17:53:59

Channel:Brightfield set at:11-Jul-2019 17:53:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 17:53:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:53:59

Channel configuration set to: GFP: 11-Jul-2019 17:54:01

Channel:GFP set at:11-Jul-2019 17:54:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 17:54:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:54:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:18 completed at:11-Jul-2019 17:54:03

Time since start of timelapse:5189.1791

PFS is locked

Time to next time point:210.8638



------Time point_19------

Memory available for all arrays: 61071179776

Memory used by Matlab: 2199101440

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:35

Channel:Brightfield set at:11-Jul-2019 17:57:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 11-Jul-2019 17:57:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:57:36

Channel configuration set to: GFP: 11-Jul-2019 17:57:37

Channel:GFP set at:11-Jul-2019 17:57:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 11-Jul-2019 17:57:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:57:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:40

Channel:Brightfield set at:11-Jul-2019 17:57:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 17:57:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:57:40

Channel configuration set to: GFP: 11-Jul-2019 17:57:41

Channel:GFP set at:11-Jul-2019 17:57:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 17:57:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:57:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:44

Channel:Brightfield set at:11-Jul-2019 17:57:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2501. 11-Jul-2019 17:57:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:57:45

Channel configuration set to: GFP: 11-Jul-2019 17:57:46

Channel:GFP set at:11-Jul-2019 17:57:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3386.2501. 11-Jul-2019 17:57:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:57:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:49

Channel:Brightfield set at:11-Jul-2019 17:57:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5001. 11-Jul-2019 17:57:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:57:50

Channel configuration set to: GFP: 11-Jul-2019 17:57:51

Channel:GFP set at:11-Jul-2019 17:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 17:57:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:57:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:54

Channel:Brightfield set at:11-Jul-2019 17:57:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 17:57:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:57:54

Channel configuration set to: GFP: 11-Jul-2019 17:57:55

Channel:GFP set at:11-Jul-2019 17:57:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 17:57:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:57:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 17:57:59

Channel:Brightfield set at:11-Jul-2019 17:57:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 17:57:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:57:59

Channel configuration set to: GFP: 11-Jul-2019 17:58:00

Channel:GFP set at:11-Jul-2019 17:58:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 17:58:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:02

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.525

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:04

Channel:Brightfield set at:11-Jul-2019 17:58:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 17:58:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:04

Channel configuration set to: GFP: 11-Jul-2019 17:58:05

Channel:GFP set at:11-Jul-2019 17:58:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 17:58:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:09

Channel:Brightfield set at:11-Jul-2019 17:58:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 17:58:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:09

Channel configuration set to: GFP: 11-Jul-2019 17:58:10

Channel:GFP set at:11-Jul-2019 17:58:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 17:58:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:14

Channel:Brightfield set at:11-Jul-2019 17:58:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 17:58:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:14

Channel configuration set to: GFP: 11-Jul-2019 17:58:16

Channel:GFP set at:11-Jul-2019 17:58:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 17:58:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:19

Channel:Brightfield set at:11-Jul-2019 17:58:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 17:58:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:19

Channel configuration set to: GFP: 11-Jul-2019 17:58:21

Channel:GFP set at:11-Jul-2019 17:58:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 17:58:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:23

Channel:Brightfield set at:11-Jul-2019 17:58:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 17:58:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:24

Channel configuration set to: GFP: 11-Jul-2019 17:58:25

Channel:GFP set at:11-Jul-2019 17:58:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 17:58:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:25

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:27

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:29

Channel:Brightfield set at:11-Jul-2019 17:58:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 11-Jul-2019 17:58:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:29

Channel configuration set to: GFP: 11-Jul-2019 17:58:30

Channel:GFP set at:11-Jul-2019 17:58:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 11-Jul-2019 17:58:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:32

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-3.925

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:34

Channel:Brightfield set at:11-Jul-2019 17:58:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 11-Jul-2019 17:58:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:34

Channel configuration set to: GFP: 11-Jul-2019 17:58:36

Channel:GFP set at:11-Jul-2019 17:58:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 11-Jul-2019 17:58:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.1

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:39

Channel:Brightfield set at:11-Jul-2019 17:58:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 17:58:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:39

Channel configuration set to: GFP: 11-Jul-2019 17:58:40

Channel:GFP set at:11-Jul-2019 17:58:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 17:58:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:42

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.1

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:43

Channel:Brightfield set at:11-Jul-2019 17:58:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4251. 11-Jul-2019 17:58:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:43

Channel configuration set to: GFP: 11-Jul-2019 17:58:45

Channel:GFP set at:11-Jul-2019 17:58:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 17:58:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:45

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.35

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:48

Channel:Brightfield set at:11-Jul-2019 17:58:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 17:58:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:49

Channel configuration set to: GFP: 11-Jul-2019 17:58:50

Channel:GFP set at:11-Jul-2019 17:58:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 17:58:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:50

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.5

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:53

Channel:Brightfield set at:11-Jul-2019 17:58:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3751. 11-Jul-2019 17:58:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:53

Channel configuration set to: GFP: 11-Jul-2019 17:58:54

Channel:GFP set at:11-Jul-2019 17:58:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3751. 11-Jul-2019 17:58:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:55

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 17:58:58

Channel:Brightfield set at:11-Jul-2019 17:58:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 17:58:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:58:58

Channel configuration set to: GFP: 11-Jul-2019 17:59:00

Channel:GFP set at:11-Jul-2019 17:59:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 17:59:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 17:59:00

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:19 completed at:11-Jul-2019 17:59:01

Time since start of timelapse:5488.0607

PFS is locked

Time to next time point:211.9836



------Time point_20------

Memory available for all arrays: 61073600512

Memory used by Matlab: 2199343104

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:02:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.425

Channel configuration set to: Brightfield: 11-Jul-2019 18:02:35

Channel:Brightfield set at:11-Jul-2019 18:02:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 18:02:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:02:36

Channel configuration set to: GFP: 11-Jul-2019 18:02:37

Channel:GFP set at:11-Jul-2019 18:02:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 18:02:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:02:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:02:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 11-Jul-2019 18:02:40

Channel:Brightfield set at:11-Jul-2019 18:02:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 18:02:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:02:41

Channel configuration set to: GFP: 11-Jul-2019 18:02:42

Channel:GFP set at:11-Jul-2019 18:02:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 18:02:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:02:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:02:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 11-Jul-2019 18:02:45

Channel:Brightfield set at:11-Jul-2019 18:02:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 18:02:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:02:46

Channel configuration set to: GFP: 11-Jul-2019 18:02:47

Channel:GFP set at:11-Jul-2019 18:02:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 18:02:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:02:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:02:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 11-Jul-2019 18:02:50

Channel:Brightfield set at:11-Jul-2019 18:02:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 18:02:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:02:50

Channel configuration set to: GFP: 11-Jul-2019 18:02:51

Channel:GFP set at:11-Jul-2019 18:02:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 18:02:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:02:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:02:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 18:02:55

Channel:Brightfield set at:11-Jul-2019 18:02:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 18:02:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:02:55

Channel configuration set to: GFP: 11-Jul-2019 18:02:56

Channel:GFP set at:11-Jul-2019 18:02:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 18:02:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:02:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:02:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:00

Channel:Brightfield set at:11-Jul-2019 18:03:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 18:03:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:00

Channel configuration set to: GFP: 11-Jul-2019 18:03:02

Channel:GFP set at:11-Jul-2019 18:03:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 18:03:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:05

Channel:Brightfield set at:11-Jul-2019 18:03:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 18:03:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:05

Channel configuration set to: GFP: 11-Jul-2019 18:03:06

Channel:GFP set at:11-Jul-2019 18:03:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 18:03:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:10

Channel:Brightfield set at:11-Jul-2019 18:03:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 11-Jul-2019 18:03:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:10

Channel configuration set to: GFP: 11-Jul-2019 18:03:12

Channel:GFP set at:11-Jul-2019 18:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 11-Jul-2019 18:03:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.475

PFS not locked. Waiting for lock...

PFS took 0.050000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:15

Channel:Brightfield set at:11-Jul-2019 18:03:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 18:03:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:15

Channel configuration set to: GFP: 11-Jul-2019 18:03:16

Channel:GFP set at:11-Jul-2019 18:03:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 11-Jul-2019 18:03:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:20

Channel:Brightfield set at:11-Jul-2019 18:03:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 18:03:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:20

Channel configuration set to: GFP: 11-Jul-2019 18:03:22

Channel:GFP set at:11-Jul-2019 18:03:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 18:03:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:25

Channel:Brightfield set at:11-Jul-2019 18:03:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 18:03:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:25

Channel configuration set to: GFP: 11-Jul-2019 18:03:26

Channel:GFP set at:11-Jul-2019 18:03:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 18:03:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:29

Channel:Brightfield set at:11-Jul-2019 18:03:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 18:03:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:30

Channel configuration set to: GFP: 11-Jul-2019 18:03:31

Channel:GFP set at:11-Jul-2019 18:03:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 18:03:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.125

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:35

Channel:Brightfield set at:11-Jul-2019 18:03:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 11-Jul-2019 18:03:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:35

Channel configuration set to: GFP: 11-Jul-2019 18:03:37

Channel:GFP set at:11-Jul-2019 18:03:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 18:03:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.15

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:40

Channel:Brightfield set at:11-Jul-2019 18:03:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6751. 11-Jul-2019 18:03:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:40

Channel configuration set to: GFP: 11-Jul-2019 18:03:41

Channel:GFP set at:11-Jul-2019 18:03:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6751. 11-Jul-2019 18:03:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.275

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:44

Channel:Brightfield set at:11-Jul-2019 18:03:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 18:03:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:44

Channel configuration set to: GFP: 11-Jul-2019 18:03:46

Channel:GFP set at:11-Jul-2019 18:03:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 18:03:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.5

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:49

Channel:Brightfield set at:11-Jul-2019 18:03:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 18:03:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:50

Channel configuration set to: GFP: 11-Jul-2019 18:03:51

Channel:GFP set at:11-Jul-2019 18:03:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 18:03:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.9

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:54

Channel:Brightfield set at:11-Jul-2019 18:03:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 18:03:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:55

Channel configuration set to: GFP: 11-Jul-2019 18:03:56

Channel:GFP set at:11-Jul-2019 18:03:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 18:03:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:03:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 18:03:59

Channel:Brightfield set at:11-Jul-2019 18:03:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 18:04:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:04:00

Channel configuration set to: GFP: 11-Jul-2019 18:04:01

Channel:GFP set at:11-Jul-2019 18:04:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 11-Jul-2019 18:04:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:04:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:20 completed at:11-Jul-2019 18:04:03

Time since start of timelapse:5789.5288

PFS is locked

Time to next time point:210.517



------Time point_21------

Memory available for all arrays: 61053505536

Memory used by Matlab: 2199347200

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:07:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.625

Channel configuration set to: Brightfield: 11-Jul-2019 18:07:35

Channel:Brightfield set at:11-Jul-2019 18:07:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 18:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:07:36

Channel configuration set to: GFP: 11-Jul-2019 18:07:37

Channel:GFP set at:11-Jul-2019 18:07:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 18:07:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:07:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:07:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 11-Jul-2019 18:07:40

Channel:Brightfield set at:11-Jul-2019 18:07:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 11-Jul-2019 18:07:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:07:41

Channel configuration set to: GFP: 11-Jul-2019 18:07:42

Channel:GFP set at:11-Jul-2019 18:07:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6501. 11-Jul-2019 18:07:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:07:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:07:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 11-Jul-2019 18:07:45

Channel:Brightfield set at:11-Jul-2019 18:07:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 18:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:07:46

Channel configuration set to: GFP: 11-Jul-2019 18:07:47

Channel:GFP set at:11-Jul-2019 18:07:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 18:07:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:07:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:07:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.525

Channel configuration set to: Brightfield: 11-Jul-2019 18:07:50

Channel:Brightfield set at:11-Jul-2019 18:07:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 18:07:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:07:50

Channel configuration set to: GFP: 11-Jul-2019 18:07:51

Channel:GFP set at:11-Jul-2019 18:07:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 18:07:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:07:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:07:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 11-Jul-2019 18:07:55

Channel:Brightfield set at:11-Jul-2019 18:07:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 18:07:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:07:55

Channel configuration set to: GFP: 11-Jul-2019 18:07:57

Channel:GFP set at:11-Jul-2019 18:07:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 18:07:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:07:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:07:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-6.925

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:00

Channel:Brightfield set at:11-Jul-2019 18:08:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 18:08:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:00

Channel configuration set to: GFP: 11-Jul-2019 18:08:01

Channel:GFP set at:11-Jul-2019 18:08:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 18:08:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.7

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:05

Channel:Brightfield set at:11-Jul-2019 18:08:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 18:08:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:05

Channel configuration set to: GFP: 11-Jul-2019 18:08:06

Channel:GFP set at:11-Jul-2019 18:08:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 18:08:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:09

Channel:Brightfield set at:11-Jul-2019 18:08:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 18:08:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:09

Channel configuration set to: GFP: 11-Jul-2019 18:08:11

Channel:GFP set at:11-Jul-2019 18:08:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 18:08:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:14

Channel:Brightfield set at:11-Jul-2019 18:08:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 18:08:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:14

Channel configuration set to: GFP: 11-Jul-2019 18:08:15

Channel:GFP set at:11-Jul-2019 18:08:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 18:08:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:19

Channel:Brightfield set at:11-Jul-2019 18:08:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 11-Jul-2019 18:08:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:19

Channel configuration set to: GFP: 11-Jul-2019 18:08:21

Channel:GFP set at:11-Jul-2019 18:08:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 11-Jul-2019 18:08:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:24

Channel:Brightfield set at:11-Jul-2019 18:08:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2501. 11-Jul-2019 18:08:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:24

Channel configuration set to: GFP: 11-Jul-2019 18:08:25

Channel:GFP set at:11-Jul-2019 18:08:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2251. 11-Jul-2019 18:08:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:27

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:28

Channel:Brightfield set at:11-Jul-2019 18:08:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4001. 11-Jul-2019 18:08:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:29

Channel configuration set to: GFP: 11-Jul-2019 18:08:30

Channel:GFP set at:11-Jul-2019 18:08:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 11-Jul-2019 18:08:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:30

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:32

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:34

Channel:Brightfield set at:11-Jul-2019 18:08:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 18:08:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:34

Channel configuration set to: GFP: 11-Jul-2019 18:08:35

Channel:GFP set at:11-Jul-2019 18:08:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 18:08:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.275

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:39

Channel:Brightfield set at:11-Jul-2019 18:08:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 18:08:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:39

Channel configuration set to: GFP: 11-Jul-2019 18:08:40

Channel:GFP set at:11-Jul-2019 18:08:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 18:08:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:42

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:43

Channel:Brightfield set at:11-Jul-2019 18:08:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 18:08:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:43

Channel configuration set to: GFP: 11-Jul-2019 18:08:45

Channel:GFP set at:11-Jul-2019 18:08:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 18:08:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:45

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.725

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:48

Channel:Brightfield set at:11-Jul-2019 18:08:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 18:08:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:48

Channel configuration set to: GFP: 11-Jul-2019 18:08:50

Channel:GFP set at:11-Jul-2019 18:08:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 18:08:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:50

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.2

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:53

Channel:Brightfield set at:11-Jul-2019 18:08:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5751. 11-Jul-2019 18:08:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:53

Channel configuration set to: GFP: 11-Jul-2019 18:08:55

Channel:GFP set at:11-Jul-2019 18:08:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5751. 11-Jul-2019 18:08:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:55

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:56

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 18:08:58

Channel:Brightfield set at:11-Jul-2019 18:08:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 18:08:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:08:58

Channel configuration set to: GFP: 11-Jul-2019 18:09:00

Channel:GFP set at:11-Jul-2019 18:09:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6251. 11-Jul-2019 18:09:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:09:00

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:21 completed at:11-Jul-2019 18:09:02

Time since start of timelapse:6088.2083

PFS is locked

Time to next time point:211.8388



------Time point_22------

Memory available for all arrays: 61067116544

Memory used by Matlab: 2199351296

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.725

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:35

Channel:Brightfield set at:11-Jul-2019 18:12:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 18:12:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:12:35

Channel configuration set to: GFP: 11-Jul-2019 18:12:37

Channel:GFP set at:11-Jul-2019 18:12:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 18:12:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:12:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:40

Channel:Brightfield set at:11-Jul-2019 18:12:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 11-Jul-2019 18:12:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:12:40

Channel configuration set to: GFP: 11-Jul-2019 18:12:42

Channel:GFP set at:11-Jul-2019 18:12:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 11-Jul-2019 18:12:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:12:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:44

Channel:Brightfield set at:11-Jul-2019 18:12:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.8001. 11-Jul-2019 18:12:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:12:45

Channel configuration set to: GFP: 11-Jul-2019 18:12:46

Channel:GFP set at:11-Jul-2019 18:12:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.8251. 11-Jul-2019 18:12:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:12:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:49

Channel:Brightfield set at:11-Jul-2019 18:12:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 18:12:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:12:49

Channel configuration set to: GFP: 11-Jul-2019 18:12:51

Channel:GFP set at:11-Jul-2019 18:12:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 18:12:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:12:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:54

Channel:Brightfield set at:11-Jul-2019 18:12:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 18:12:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:12:55

Channel configuration set to: GFP: 11-Jul-2019 18:12:56

Channel:GFP set at:11-Jul-2019 18:12:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 18:12:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:12:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 18:12:59

Channel:Brightfield set at:11-Jul-2019 18:12:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 18:12:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:12:59

Channel configuration set to: GFP: 11-Jul-2019 18:13:00

Channel:GFP set at:11-Jul-2019 18:13:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 18:13:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:02

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:04

Channel:Brightfield set at:11-Jul-2019 18:13:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 18:13:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:04

Channel configuration set to: GFP: 11-Jul-2019 18:13:05

Channel:GFP set at:11-Jul-2019 18:13:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 18:13:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:09

Channel:Brightfield set at:11-Jul-2019 18:13:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 18:13:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:09

Channel configuration set to: GFP: 11-Jul-2019 18:13:10

Channel:GFP set at:11-Jul-2019 18:13:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 11-Jul-2019 18:13:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.075

PFS not locked. Waiting for lock...

PFS took 0.800000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:14

Channel:Brightfield set at:11-Jul-2019 18:13:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 18:13:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:15

Channel configuration set to: GFP: 11-Jul-2019 18:13:16

Channel:GFP set at:11-Jul-2019 18:13:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 18:13:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:19

Channel:Brightfield set at:11-Jul-2019 18:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 18:13:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:20

Channel configuration set to: GFP: 11-Jul-2019 18:13:21

Channel:GFP set at:11-Jul-2019 18:13:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 18:13:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:24

Channel:Brightfield set at:11-Jul-2019 18:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2751. 11-Jul-2019 18:13:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:24

Channel configuration set to: GFP: 11-Jul-2019 18:13:26

Channel:GFP set at:11-Jul-2019 18:13:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2501. 11-Jul-2019 18:13:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:29

Channel:Brightfield set at:11-Jul-2019 18:13:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 18:13:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:30

Channel configuration set to: GFP: 11-Jul-2019 18:13:31

Channel:GFP set at:11-Jul-2019 18:13:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 18:13:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.325

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:35

Channel:Brightfield set at:11-Jul-2019 18:13:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 18:13:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:35

Channel configuration set to: GFP: 11-Jul-2019 18:13:36

Channel:GFP set at:11-Jul-2019 18:13:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 18:13:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.4

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:39

Channel:Brightfield set at:11-Jul-2019 18:13:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 18:13:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:40

Channel configuration set to: GFP: 11-Jul-2019 18:13:41

Channel:GFP set at:11-Jul-2019 18:13:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 11-Jul-2019 18:13:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.675

PFS not locked. Waiting for lock...

PFS took 0.450000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:44

Channel:Brightfield set at:11-Jul-2019 18:13:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 18:13:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:44

Channel configuration set to: GFP: 11-Jul-2019 18:13:46

Channel:GFP set at:11-Jul-2019 18:13:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 18:13:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.675

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:49

Channel:Brightfield set at:11-Jul-2019 18:13:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 18:13:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:49

Channel configuration set to: GFP: 11-Jul-2019 18:13:51

Channel:GFP set at:11-Jul-2019 18:13:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 18:13:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.2

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:54

Channel:Brightfield set at:11-Jul-2019 18:13:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5751. 11-Jul-2019 18:13:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:54

Channel configuration set to: GFP: 11-Jul-2019 18:13:56

Channel:GFP set at:11-Jul-2019 18:13:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5751. 11-Jul-2019 18:13:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.7

Channel configuration set to: Brightfield: 11-Jul-2019 18:13:59

Channel:Brightfield set at:11-Jul-2019 18:13:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 18:13:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:13:59

Channel configuration set to: GFP: 11-Jul-2019 18:14:01

Channel:GFP set at:11-Jul-2019 18:14:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7251. 11-Jul-2019 18:14:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:14:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:22 completed at:11-Jul-2019 18:14:02

Time since start of timelapse:6389.1133

PFS is locked

Time to next time point:210.9351



------Time point_23------

Memory available for all arrays: 61064142848

Memory used by Matlab: 2203734016

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:17:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 11-Jul-2019 18:17:35

Channel:Brightfield set at:11-Jul-2019 18:17:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 18:17:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:17:36

Channel configuration set to: GFP: 11-Jul-2019 18:17:37

Channel:GFP set at:11-Jul-2019 18:17:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 18:17:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:17:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:17:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 11-Jul-2019 18:17:41

Channel:Brightfield set at:11-Jul-2019 18:17:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 18:17:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:17:41

Channel configuration set to: GFP: 11-Jul-2019 18:17:42

Channel:GFP set at:11-Jul-2019 18:17:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 18:17:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:17:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:17:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 11-Jul-2019 18:17:46

Channel:Brightfield set at:11-Jul-2019 18:17:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6501. 11-Jul-2019 18:17:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:17:46

Channel configuration set to: GFP: 11-Jul-2019 18:17:47

Channel:GFP set at:11-Jul-2019 18:17:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6501. 11-Jul-2019 18:17:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:17:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:17:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 11-Jul-2019 18:17:50

Channel:Brightfield set at:11-Jul-2019 18:17:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1751. 11-Jul-2019 18:17:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:17:51

Channel configuration set to: GFP: 11-Jul-2019 18:17:52

Channel:GFP set at:11-Jul-2019 18:17:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1751. 11-Jul-2019 18:17:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:17:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:17:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 11-Jul-2019 18:17:56

Channel:Brightfield set at:11-Jul-2019 18:17:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 18:17:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:17:56

Channel configuration set to: GFP: 11-Jul-2019 18:17:57

Channel:GFP set at:11-Jul-2019 18:17:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 18:17:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:17:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:17:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:01

Channel:Brightfield set at:11-Jul-2019 18:18:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 18:18:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:01

Channel configuration set to: GFP: 11-Jul-2019 18:18:03

Channel:GFP set at:11-Jul-2019 18:18:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 18:18:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:06

Channel:Brightfield set at:11-Jul-2019 18:18:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 18:18:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:06

Channel configuration set to: GFP: 11-Jul-2019 18:18:07

Channel:GFP set at:11-Jul-2019 18:18:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 18:18:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:11

Channel:Brightfield set at:11-Jul-2019 18:18:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 18:18:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:11

Channel configuration set to: GFP: 11-Jul-2019 18:18:13

Channel:GFP set at:11-Jul-2019 18:18:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 18:18:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:16

Channel:Brightfield set at:11-Jul-2019 18:18:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 18:18:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:17

Channel configuration set to: GFP: 11-Jul-2019 18:18:18

Channel:GFP set at:11-Jul-2019 18:18:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 18:18:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:21

Channel:Brightfield set at:11-Jul-2019 18:18:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 18:18:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:21

Channel configuration set to: GFP: 11-Jul-2019 18:18:22

Channel:GFP set at:11-Jul-2019 18:18:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 18:18:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:25

Channel:Brightfield set at:11-Jul-2019 18:18:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 18:18:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:26

Channel configuration set to: GFP: 11-Jul-2019 18:18:27

Channel:GFP set at:11-Jul-2019 18:18:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 18:18:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:30

Channel:Brightfield set at:11-Jul-2019 18:18:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 11-Jul-2019 18:18:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:30

Channel configuration set to: GFP: 11-Jul-2019 18:18:32

Channel:GFP set at:11-Jul-2019 18:18:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 11-Jul-2019 18:18:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:35

Channel:Brightfield set at:11-Jul-2019 18:18:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 11-Jul-2019 18:18:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:36

Channel configuration set to: GFP: 11-Jul-2019 18:18:37

Channel:GFP set at:11-Jul-2019 18:18:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 11-Jul-2019 18:18:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.975

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:40

Channel:Brightfield set at:11-Jul-2019 18:18:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 18:18:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:41

Channel configuration set to: GFP: 11-Jul-2019 18:18:42

Channel:GFP set at:11-Jul-2019 18:18:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 18:18:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.075

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:45

Channel:Brightfield set at:11-Jul-2019 18:18:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 18:18:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:45

Channel configuration set to: GFP: 11-Jul-2019 18:18:46

Channel:GFP set at:11-Jul-2019 18:18:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 11-Jul-2019 18:18:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.375

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:50

Channel:Brightfield set at:11-Jul-2019 18:18:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 18:18:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:50

Channel configuration set to: GFP: 11-Jul-2019 18:18:51

Channel:GFP set at:11-Jul-2019 18:18:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 18:18:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.85

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:54

Channel:Brightfield set at:11-Jul-2019 18:18:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2751. 11-Jul-2019 18:18:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:55

Channel configuration set to: GFP: 11-Jul-2019 18:18:56

Channel:GFP set at:11-Jul-2019 18:18:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2751. 11-Jul-2019 18:18:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:18:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:18:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-3.6

PFS not locked. Waiting for lock...

PFS took 0.850000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 18:19:00

Channel:Brightfield set at:11-Jul-2019 18:19:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 18:19:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:19:00

Channel configuration set to: GFP: 11-Jul-2019 18:19:01

Channel:GFP set at:11-Jul-2019 18:19:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 18:19:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:19:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:23 completed at:11-Jul-2019 18:19:03

Time since start of timelapse:6690.0622

PFS is locked

Time to next time point:209.9878



------Time point_24------

Memory available for all arrays: 61065482240

Memory used by Matlab: 2204168192

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:22:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 11-Jul-2019 18:22:35

Channel:Brightfield set at:11-Jul-2019 18:22:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 18:22:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:22:36

Channel configuration set to: GFP: 11-Jul-2019 18:22:37

Channel:GFP set at:11-Jul-2019 18:22:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 18:22:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:22:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:22:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-7.925

Channel configuration set to: Brightfield: 11-Jul-2019 18:22:40

Channel:Brightfield set at:11-Jul-2019 18:22:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 18:22:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:22:41

Channel configuration set to: GFP: 11-Jul-2019 18:22:42

Channel:GFP set at:11-Jul-2019 18:22:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 18:22:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:22:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:22:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.225

Channel configuration set to: Brightfield: 11-Jul-2019 18:22:45

Channel:Brightfield set at:11-Jul-2019 18:22:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 18:22:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:22:46

Channel configuration set to: GFP: 11-Jul-2019 18:22:47

Channel:GFP set at:11-Jul-2019 18:22:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 18:22:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:22:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:22:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.5

Channel configuration set to: Brightfield: 11-Jul-2019 18:22:50

Channel:Brightfield set at:11-Jul-2019 18:22:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 11-Jul-2019 18:22:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:22:51

Channel configuration set to: GFP: 11-Jul-2019 18:22:52

Channel:GFP set at:11-Jul-2019 18:22:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 11-Jul-2019 18:22:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:22:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:22:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 11-Jul-2019 18:22:55

Channel:Brightfield set at:11-Jul-2019 18:22:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 11-Jul-2019 18:22:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:22:56

Channel configuration set to: GFP: 11-Jul-2019 18:22:57

Channel:GFP set at:11-Jul-2019 18:22:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 18:22:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:22:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:22:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:00

Channel:Brightfield set at:11-Jul-2019 18:23:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 18:23:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:00

Channel configuration set to: GFP: 11-Jul-2019 18:23:02

Channel:GFP set at:11-Jul-2019 18:23:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 18:23:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:06

Channel:Brightfield set at:11-Jul-2019 18:23:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 18:23:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:06

Channel configuration set to: GFP: 11-Jul-2019 18:23:07

Channel:GFP set at:11-Jul-2019 18:23:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 18:23:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:11

Channel:Brightfield set at:11-Jul-2019 18:23:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 18:23:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:11

Channel configuration set to: GFP: 11-Jul-2019 18:23:12

Channel:GFP set at:11-Jul-2019 18:23:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 18:23:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.4

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:16

Channel:Brightfield set at:11-Jul-2019 18:23:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 18:23:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:16

Channel configuration set to: GFP: 11-Jul-2019 18:23:17

Channel:GFP set at:11-Jul-2019 18:23:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 18:23:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:21

Channel:Brightfield set at:11-Jul-2019 18:23:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 18:23:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:21

Channel configuration set to: GFP: 11-Jul-2019 18:23:22

Channel:GFP set at:11-Jul-2019 18:23:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 18:23:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:26

Channel:Brightfield set at:11-Jul-2019 18:23:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6501. 11-Jul-2019 18:23:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:26

Channel configuration set to: GFP: 11-Jul-2019 18:23:27

Channel:GFP set at:11-Jul-2019 18:23:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6501. 11-Jul-2019 18:23:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.075

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:30

Channel:Brightfield set at:11-Jul-2019 18:23:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 18:23:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:31

Channel configuration set to: GFP: 11-Jul-2019 18:23:32

Channel:GFP set at:11-Jul-2019 18:23:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 18:23:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.9

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:36

Channel:Brightfield set at:11-Jul-2019 18:23:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 18:23:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:36

Channel configuration set to: GFP: 11-Jul-2019 18:23:37

Channel:GFP set at:11-Jul-2019 18:23:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 18:23:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.875

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:41

Channel:Brightfield set at:11-Jul-2019 18:23:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 18:23:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:41

Channel configuration set to: GFP: 11-Jul-2019 18:23:42

Channel:GFP set at:11-Jul-2019 18:23:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9501. 11-Jul-2019 18:23:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-4.875

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:45

Channel:Brightfield set at:11-Jul-2019 18:23:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 18:23:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:45

Channel configuration set to: GFP: 11-Jul-2019 18:23:47

Channel:GFP set at:11-Jul-2019 18:23:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 18:23:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.25

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:50

Channel:Brightfield set at:11-Jul-2019 18:23:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1501. 11-Jul-2019 18:23:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:51

Channel configuration set to: GFP: 11-Jul-2019 18:23:52

Channel:GFP set at:11-Jul-2019 18:23:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1501. 11-Jul-2019 18:23:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:54

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.65

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:54

Channel:Brightfield set at:11-Jul-2019 18:23:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2751. 11-Jul-2019 18:23:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:55

Channel configuration set to: GFP: 11-Jul-2019 18:23:56

Channel:GFP set at:11-Jul-2019 18:23:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2751. 11-Jul-2019 18:23:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:23:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:23:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 18:24:00

Channel:Brightfield set at:11-Jul-2019 18:24:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 18:24:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:24:00

Channel configuration set to: GFP: 11-Jul-2019 18:24:02

Channel:GFP set at:11-Jul-2019 18:24:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8751. 11-Jul-2019 18:24:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:24:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:24 completed at:11-Jul-2019 18:24:04

Time since start of timelapse:6990.4489

PFS is locked

Time to next time point:209.6029



------Time point_25------

Memory available for all arrays: 61065453568

Memory used by Matlab: 2204168192

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:27:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 11-Jul-2019 18:27:35

Channel:Brightfield set at:11-Jul-2019 18:27:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 18:27:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:27:36

Channel configuration set to: GFP: 11-Jul-2019 18:27:37

Channel:GFP set at:11-Jul-2019 18:27:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 18:27:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:27:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:27:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-7.925

Channel configuration set to: Brightfield: 11-Jul-2019 18:27:40

Channel:Brightfield set at:11-Jul-2019 18:27:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 11-Jul-2019 18:27:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:27:40

Channel configuration set to: GFP: 11-Jul-2019 18:27:42

Channel:GFP set at:11-Jul-2019 18:27:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 11-Jul-2019 18:27:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:27:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:27:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.95

PFS not locked. Waiting for lock...

PFS took 0.800000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 18:27:45

Channel:Brightfield set at:11-Jul-2019 18:27:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 18:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:27:46

Channel configuration set to: GFP: 11-Jul-2019 18:27:47

Channel:GFP set at:11-Jul-2019 18:27:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 18:27:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:27:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:27:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 11-Jul-2019 18:27:50

Channel:Brightfield set at:11-Jul-2019 18:27:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0751. 11-Jul-2019 18:27:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:27:50

Channel configuration set to: GFP: 11-Jul-2019 18:27:52

Channel:GFP set at:11-Jul-2019 18:27:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 18:27:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:27:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:27:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 18:27:55

Channel:Brightfield set at:11-Jul-2019 18:27:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 18:27:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:27:55

Channel configuration set to: GFP: 11-Jul-2019 18:27:57

Channel:GFP set at:11-Jul-2019 18:27:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 18:27:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:27:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:27:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.2

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:00

Channel:Brightfield set at:11-Jul-2019 18:28:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 18:28:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:00

Channel configuration set to: GFP: 11-Jul-2019 18:28:01

Channel:GFP set at:11-Jul-2019 18:28:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 18:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.35

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:05

Channel:Brightfield set at:11-Jul-2019 18:28:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 11-Jul-2019 18:28:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:06

Channel configuration set to: GFP: 11-Jul-2019 18:28:07

Channel:GFP set at:11-Jul-2019 18:28:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 11-Jul-2019 18:28:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:10

Channel:Brightfield set at:11-Jul-2019 18:28:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 18:28:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:11

Channel configuration set to: GFP: 11-Jul-2019 18:28:12

Channel:GFP set at:11-Jul-2019 18:28:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 18:28:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:15

Channel:Brightfield set at:11-Jul-2019 18:28:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 18:28:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:16

Channel configuration set to: GFP: 11-Jul-2019 18:28:17

Channel:GFP set at:11-Jul-2019 18:28:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 18:28:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:20

Channel:Brightfield set at:11-Jul-2019 18:28:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 18:28:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:21

Channel configuration set to: GFP: 11-Jul-2019 18:28:22

Channel:GFP set at:11-Jul-2019 18:28:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 18:28:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:26

Channel:Brightfield set at:11-Jul-2019 18:28:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 18:28:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:26

Channel configuration set to: GFP: 11-Jul-2019 18:28:27

Channel:GFP set at:11-Jul-2019 18:28:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 18:28:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:30

Channel:Brightfield set at:11-Jul-2019 18:28:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 11-Jul-2019 18:28:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:31

Channel configuration set to: GFP: 11-Jul-2019 18:28:32

Channel:GFP set at:11-Jul-2019 18:28:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 11-Jul-2019 18:28:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.075

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:35

Channel:Brightfield set at:11-Jul-2019 18:28:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 11-Jul-2019 18:28:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:35

Channel configuration set to: GFP: 11-Jul-2019 18:28:37

Channel:GFP set at:11-Jul-2019 18:28:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3751. 11-Jul-2019 18:28:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-4.975

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:40

Channel:Brightfield set at:11-Jul-2019 18:28:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 18:28:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:40

Channel configuration set to: GFP: 11-Jul-2019 18:28:42

Channel:GFP set at:11-Jul-2019 18:28:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 18:28:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-4.775

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:45

Channel:Brightfield set at:11-Jul-2019 18:28:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 18:28:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:45

Channel configuration set to: GFP: 11-Jul-2019 18:28:46

Channel:GFP set at:11-Jul-2019 18:28:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 18:28:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.825

PFS not locked. Waiting for lock...

PFS took 0.700000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:50

Channel:Brightfield set at:11-Jul-2019 18:28:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2001. 11-Jul-2019 18:28:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:50

Channel configuration set to: GFP: 11-Jul-2019 18:28:51

Channel:GFP set at:11-Jul-2019 18:28:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2001. 11-Jul-2019 18:28:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-3.875

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:55

Channel:Brightfield set at:11-Jul-2019 18:28:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.8751. 11-Jul-2019 18:28:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:55

Channel configuration set to: GFP: 11-Jul-2019 18:28:56

Channel:GFP set at:11-Jul-2019 18:28:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.8501. 11-Jul-2019 18:28:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:28:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:28:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 18:29:00

Channel:Brightfield set at:11-Jul-2019 18:29:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 18:29:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:29:00

Channel configuration set to: GFP: 11-Jul-2019 18:29:02

Channel:GFP set at:11-Jul-2019 18:29:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 18:29:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:29:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:25 completed at:11-Jul-2019 18:29:04

Time since start of timelapse:7290.2978

PFS is locked

Time to next time point:209.7556



------Time point_26------

Memory available for all arrays: 61064859648

Memory used by Matlab: 2204528640

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:32:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 11-Jul-2019 18:32:35

Channel:Brightfield set at:11-Jul-2019 18:32:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 18:32:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:32:36

Channel configuration set to: GFP: 11-Jul-2019 18:32:37

Channel:GFP set at:11-Jul-2019 18:32:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 18:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:32:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:32:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 11-Jul-2019 18:32:40

Channel:Brightfield set at:11-Jul-2019 18:32:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 11-Jul-2019 18:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:32:41

Channel configuration set to: GFP: 11-Jul-2019 18:32:42

Channel:GFP set at:11-Jul-2019 18:32:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8251. 11-Jul-2019 18:32:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:32:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:32:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 18:32:45

Channel:Brightfield set at:11-Jul-2019 18:32:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7251. 11-Jul-2019 18:32:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:32:45

Channel configuration set to: GFP: 11-Jul-2019 18:32:46

Channel:GFP set at:11-Jul-2019 18:32:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7501. 11-Jul-2019 18:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:32:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:32:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.65

Channel configuration set to: Brightfield: 11-Jul-2019 18:32:50

Channel:Brightfield set at:11-Jul-2019 18:32:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 11-Jul-2019 18:32:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:32:50

Channel configuration set to: GFP: 11-Jul-2019 18:32:51

Channel:GFP set at:11-Jul-2019 18:32:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 11-Jul-2019 18:32:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:32:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:32:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 11-Jul-2019 18:32:55

Channel:Brightfield set at:11-Jul-2019 18:32:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 18:32:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:32:55

Channel configuration set to: GFP: 11-Jul-2019 18:32:57

Channel:GFP set at:11-Jul-2019 18:32:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 18:32:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:32:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:32:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:00

Channel:Brightfield set at:11-Jul-2019 18:33:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 18:33:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:00

Channel configuration set to: GFP: 11-Jul-2019 18:33:02

Channel:GFP set at:11-Jul-2019 18:33:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 18:33:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:05

Channel:Brightfield set at:11-Jul-2019 18:33:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 18:33:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:05

Channel configuration set to: GFP: 11-Jul-2019 18:33:07

Channel:GFP set at:11-Jul-2019 18:33:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 18:33:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:10

Channel:Brightfield set at:11-Jul-2019 18:33:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 11-Jul-2019 18:33:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:10

Channel configuration set to: GFP: 11-Jul-2019 18:33:11

Channel:GFP set at:11-Jul-2019 18:33:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 18:33:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:14

Channel:Brightfield set at:11-Jul-2019 18:33:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 18:33:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:15

Channel configuration set to: GFP: 11-Jul-2019 18:33:16

Channel:GFP set at:11-Jul-2019 18:33:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 18:33:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.075

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:19

Channel:Brightfield set at:11-Jul-2019 18:33:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 18:33:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:19

Channel configuration set to: GFP: 11-Jul-2019 18:33:21

Channel:GFP set at:11-Jul-2019 18:33:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 18:33:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:24

Channel:Brightfield set at:11-Jul-2019 18:33:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1251. 11-Jul-2019 18:33:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:24

Channel configuration set to: GFP: 11-Jul-2019 18:33:25

Channel:GFP set at:11-Jul-2019 18:33:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1251. 11-Jul-2019 18:33:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:27

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:28

Channel:Brightfield set at:11-Jul-2019 18:33:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 18:33:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:29

Channel configuration set to: GFP: 11-Jul-2019 18:33:30

Channel:GFP set at:11-Jul-2019 18:33:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 18:33:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:30

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:32

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:34

Channel:Brightfield set at:11-Jul-2019 18:33:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 18:33:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:34

Channel configuration set to: GFP: 11-Jul-2019 18:33:36

Channel:GFP set at:11-Jul-2019 18:33:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 18:33:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.2

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:39

Channel:Brightfield set at:11-Jul-2019 18:33:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 18:33:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:39

Channel configuration set to: GFP: 11-Jul-2019 18:33:41

Channel:GFP set at:11-Jul-2019 18:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 18:33:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:42

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:43

Channel:Brightfield set at:11-Jul-2019 18:33:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 18:33:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:44

Channel configuration set to: GFP: 11-Jul-2019 18:33:45

Channel:GFP set at:11-Jul-2019 18:33:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 18:33:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:45

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.575

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:48

Channel:Brightfield set at:11-Jul-2019 18:33:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 18:33:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:49

Channel configuration set to: GFP: 11-Jul-2019 18:33:50

Channel:GFP set at:11-Jul-2019 18:33:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 18:33:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:50

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5

PFS not locked. Waiting for lock...

PFS took 0.450000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:53

Channel:Brightfield set at:11-Jul-2019 18:33:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6501. 11-Jul-2019 18:33:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:53

Channel configuration set to: GFP: 11-Jul-2019 18:33:55

Channel:GFP set at:11-Jul-2019 18:33:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6751. 11-Jul-2019 18:33:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:55

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 18:33:58

Channel:Brightfield set at:11-Jul-2019 18:33:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 18:33:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:33:59

Channel configuration set to: GFP: 11-Jul-2019 18:34:00

Channel:GFP set at:11-Jul-2019 18:34:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 18:34:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:34:00

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:26 completed at:11-Jul-2019 18:34:02

Time since start of timelapse:7588.6457

PFS is locked

Time to next time point:211.4089



------Time point_27------

Memory available for all arrays: 61063700480

Memory used by Matlab: 2204626944

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:35

Channel:Brightfield set at:11-Jul-2019 18:37:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 18:37:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:37:35

Channel configuration set to: GFP: 11-Jul-2019 18:37:37

Channel:GFP set at:11-Jul-2019 18:37:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 18:37:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:37:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:40

Channel:Brightfield set at:11-Jul-2019 18:37:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 18:37:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:37:40

Channel configuration set to: GFP: 11-Jul-2019 18:37:42

Channel:GFP set at:11-Jul-2019 18:37:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 11-Jul-2019 18:37:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:37:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.55

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:45

Channel:Brightfield set at:11-Jul-2019 18:37:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 18:37:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:37:45

Channel configuration set to: GFP: 11-Jul-2019 18:37:47

Channel:GFP set at:11-Jul-2019 18:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 18:37:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:37:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.525

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:50

Channel:Brightfield set at:11-Jul-2019 18:37:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 18:37:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:37:50

Channel configuration set to: GFP: 11-Jul-2019 18:37:52

Channel:GFP set at:11-Jul-2019 18:37:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0751. 11-Jul-2019 18:37:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:37:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:55

Channel:Brightfield set at:11-Jul-2019 18:37:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3751. 11-Jul-2019 18:37:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:37:55

Channel configuration set to: GFP: 11-Jul-2019 18:37:56

Channel:GFP set at:11-Jul-2019 18:37:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3751. 11-Jul-2019 18:37:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:37:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.55

Channel configuration set to: Brightfield: 11-Jul-2019 18:37:59

Channel:Brightfield set at:11-Jul-2019 18:37:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 11-Jul-2019 18:38:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:00

Channel configuration set to: GFP: 11-Jul-2019 18:38:01

Channel:GFP set at:11-Jul-2019 18:38:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 11-Jul-2019 18:38:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:04

Channel:Brightfield set at:11-Jul-2019 18:38:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 18:38:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:05

Channel configuration set to: GFP: 11-Jul-2019 18:38:06

Channel:GFP set at:11-Jul-2019 18:38:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 18:38:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:09

Channel:Brightfield set at:11-Jul-2019 18:38:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 18:38:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:10

Channel configuration set to: GFP: 11-Jul-2019 18:38:11

Channel:GFP set at:11-Jul-2019 18:38:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 18:38:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:15

Channel:Brightfield set at:11-Jul-2019 18:38:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 18:38:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:15

Channel configuration set to: GFP: 11-Jul-2019 18:38:16

Channel:GFP set at:11-Jul-2019 18:38:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 18:38:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.175

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:19

Channel:Brightfield set at:11-Jul-2019 18:38:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 11-Jul-2019 18:38:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:19

Channel configuration set to: GFP: 11-Jul-2019 18:38:21

Channel:GFP set at:11-Jul-2019 18:38:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 18:38:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:24

Channel:Brightfield set at:11-Jul-2019 18:38:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 18:38:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:24

Channel configuration set to: GFP: 11-Jul-2019 18:38:25

Channel:GFP set at:11-Jul-2019 18:38:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 18:38:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:27

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.4

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:28

Channel:Brightfield set at:11-Jul-2019 18:38:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 11-Jul-2019 18:38:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:28

Channel configuration set to: GFP: 11-Jul-2019 18:38:30

Channel:GFP set at:11-Jul-2019 18:38:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 11-Jul-2019 18:38:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:30

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:32

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:34

Channel:Brightfield set at:11-Jul-2019 18:38:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1501. 11-Jul-2019 18:38:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:34

Channel configuration set to: GFP: 11-Jul-2019 18:38:36

Channel:GFP set at:11-Jul-2019 18:38:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 18:38:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.325

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:39

Channel:Brightfield set at:11-Jul-2019 18:38:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 11-Jul-2019 18:38:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:39

Channel configuration set to: GFP: 11-Jul-2019 18:38:40

Channel:GFP set at:11-Jul-2019 18:38:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 11-Jul-2019 18:38:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:42

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:43

Channel:Brightfield set at:11-Jul-2019 18:38:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 18:38:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:43

Channel configuration set to: GFP: 11-Jul-2019 18:38:45

Channel:GFP set at:11-Jul-2019 18:38:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 18:38:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:45

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.65

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:48

Channel:Brightfield set at:11-Jul-2019 18:38:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7501. 11-Jul-2019 18:38:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:49

Channel configuration set to: GFP: 11-Jul-2019 18:38:50

Channel:GFP set at:11-Jul-2019 18:38:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 18:38:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:50

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.35

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:53

Channel:Brightfield set at:11-Jul-2019 18:38:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4251. 11-Jul-2019 18:38:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:53

Channel configuration set to: GFP: 11-Jul-2019 18:38:55

Channel:GFP set at:11-Jul-2019 18:38:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4251. 11-Jul-2019 18:38:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:55

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 11-Jul-2019 18:38:58

Channel:Brightfield set at:11-Jul-2019 18:38:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 18:38:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:38:58

Channel configuration set to: GFP: 11-Jul-2019 18:39:00

Channel:GFP set at:11-Jul-2019 18:39:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 18:39:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:39:00

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:27 completed at:11-Jul-2019 18:39:02

Time since start of timelapse:7888.4985

PFS is locked

Time to next time point:211.5576



------Time point_28------

Memory available for all arrays: 61061181440

Memory used by Matlab: 2206830592

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.85

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:35

Channel:Brightfield set at:11-Jul-2019 18:42:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4751. 11-Jul-2019 18:42:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:42:35

Channel configuration set to: GFP: 11-Jul-2019 18:42:37

Channel:GFP set at:11-Jul-2019 18:42:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 18:42:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:42:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:40

Channel:Brightfield set at:11-Jul-2019 18:42:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 18:42:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:42:40

Channel configuration set to: GFP: 11-Jul-2019 18:42:41

Channel:GFP set at:11-Jul-2019 18:42:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 18:42:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:42:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:44

Channel:Brightfield set at:11-Jul-2019 18:42:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6251. 11-Jul-2019 18:42:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:42:45

Channel configuration set to: GFP: 11-Jul-2019 18:42:46

Channel:GFP set at:11-Jul-2019 18:42:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6251. 11-Jul-2019 18:42:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:42:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.85

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:49

Channel:Brightfield set at:11-Jul-2019 18:42:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 11-Jul-2019 18:42:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:42:50

Channel configuration set to: GFP: 11-Jul-2019 18:42:51

Channel:GFP set at:11-Jul-2019 18:42:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7751. 11-Jul-2019 18:42:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:42:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.525

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:55

Channel:Brightfield set at:11-Jul-2019 18:42:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 18:42:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:42:55

Channel configuration set to: GFP: 11-Jul-2019 18:42:56

Channel:GFP set at:11-Jul-2019 18:42:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 18:42:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:42:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 11-Jul-2019 18:42:59

Channel:Brightfield set at:11-Jul-2019 18:42:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 18:42:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:42:59

Channel configuration set to: GFP: 11-Jul-2019 18:43:01

Channel:GFP set at:11-Jul-2019 18:43:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 18:43:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:04

Channel:Brightfield set at:11-Jul-2019 18:43:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 11-Jul-2019 18:43:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:05

Channel configuration set to: GFP: 11-Jul-2019 18:43:06

Channel:GFP set at:11-Jul-2019 18:43:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 18:43:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:10

Channel:Brightfield set at:11-Jul-2019 18:43:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 11-Jul-2019 18:43:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:10

Channel configuration set to: GFP: 11-Jul-2019 18:43:11

Channel:GFP set at:11-Jul-2019 18:43:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 11-Jul-2019 18:43:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:14

Channel:Brightfield set at:11-Jul-2019 18:43:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2001. 11-Jul-2019 18:43:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:15

Channel configuration set to: GFP: 11-Jul-2019 18:43:16

Channel:GFP set at:11-Jul-2019 18:43:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2001. 11-Jul-2019 18:43:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:19

Channel:Brightfield set at:11-Jul-2019 18:43:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 18:43:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:20

Channel configuration set to: GFP: 11-Jul-2019 18:43:21

Channel:GFP set at:11-Jul-2019 18:43:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 18:43:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:24

Channel:Brightfield set at:11-Jul-2019 18:43:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 11-Jul-2019 18:43:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:25

Channel configuration set to: GFP: 11-Jul-2019 18:43:26

Channel:GFP set at:11-Jul-2019 18:43:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 11-Jul-2019 18:43:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:30

Channel:Brightfield set at:11-Jul-2019 18:43:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 11-Jul-2019 18:43:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:30

Channel configuration set to: GFP: 11-Jul-2019 18:43:31

Channel:GFP set at:11-Jul-2019 18:43:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 11-Jul-2019 18:43:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:35

Channel:Brightfield set at:11-Jul-2019 18:43:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 18:43:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:35

Channel configuration set to: GFP: 11-Jul-2019 18:43:36

Channel:GFP set at:11-Jul-2019 18:43:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 18:43:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.475

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:39

Channel:Brightfield set at:11-Jul-2019 18:43:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 18:43:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:40

Channel configuration set to: GFP: 11-Jul-2019 18:43:41

Channel:GFP set at:11-Jul-2019 18:43:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 18:43:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:44

Channel:Brightfield set at:11-Jul-2019 18:43:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 11-Jul-2019 18:43:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:45

Channel configuration set to: GFP: 11-Jul-2019 18:43:46

Channel:GFP set at:11-Jul-2019 18:43:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 18:43:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.725

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:49

Channel:Brightfield set at:11-Jul-2019 18:43:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 11-Jul-2019 18:43:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:50

Channel configuration set to: GFP: 11-Jul-2019 18:43:51

Channel:GFP set at:11-Jul-2019 18:43:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 18:43:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.175

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:54

Channel:Brightfield set at:11-Jul-2019 18:43:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 11-Jul-2019 18:43:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:54

Channel configuration set to: GFP: 11-Jul-2019 18:43:55

Channel:GFP set at:11-Jul-2019 18:43:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6501. 11-Jul-2019 18:43:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 18:43:59

Channel:Brightfield set at:11-Jul-2019 18:43:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 18:43:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:43:59

Channel configuration set to: GFP: 11-Jul-2019 18:44:01

Channel:GFP set at:11-Jul-2019 18:44:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3751. 11-Jul-2019 18:44:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:44:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:28 completed at:11-Jul-2019 18:44:03

Time since start of timelapse:8189.3322

PFS is locked

Time to next time point:210.7256



------Time point_29------

Memory available for all arrays: 61062873088

Memory used by Matlab: 2207080448

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:35

Channel:Brightfield set at:11-Jul-2019 18:47:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3251. 11-Jul-2019 18:47:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:47:35

Channel configuration set to: GFP: 11-Jul-2019 18:47:37

Channel:GFP set at:11-Jul-2019 18:47:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3251. 11-Jul-2019 18:47:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:47:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.575

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:40

Channel:Brightfield set at:11-Jul-2019 18:47:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 18:47:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:47:40

Channel configuration set to: GFP: 11-Jul-2019 18:47:41

Channel:GFP set at:11-Jul-2019 18:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 18:47:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:47:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:44

Channel:Brightfield set at:11-Jul-2019 18:47:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6501. 11-Jul-2019 18:47:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:47:45

Channel configuration set to: GFP: 11-Jul-2019 18:47:46

Channel:GFP set at:11-Jul-2019 18:47:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.6501. 11-Jul-2019 18:47:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:47:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:49

Channel:Brightfield set at:11-Jul-2019 18:47:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 18:47:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:47:50

Channel configuration set to: GFP: 11-Jul-2019 18:47:51

Channel:GFP set at:11-Jul-2019 18:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 18:47:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:47:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:55

Channel:Brightfield set at:11-Jul-2019 18:47:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3001. 11-Jul-2019 18:47:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:47:55

Channel configuration set to: GFP: 11-Jul-2019 18:47:56

Channel:GFP set at:11-Jul-2019 18:47:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3001. 11-Jul-2019 18:47:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:47:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.775

Channel configuration set to: Brightfield: 11-Jul-2019 18:47:59

Channel:Brightfield set at:11-Jul-2019 18:47:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 11-Jul-2019 18:47:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:47:59

Channel configuration set to: GFP: 11-Jul-2019 18:48:01

Channel:GFP set at:11-Jul-2019 18:48:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 18:48:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:05

Channel:Brightfield set at:11-Jul-2019 18:48:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 18:48:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:05

Channel configuration set to: GFP: 11-Jul-2019 18:48:06

Channel:GFP set at:11-Jul-2019 18:48:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 18:48:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:09

Channel:Brightfield set at:11-Jul-2019 18:48:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 18:48:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:10

Channel configuration set to: GFP: 11-Jul-2019 18:48:11

Channel:GFP set at:11-Jul-2019 18:48:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 18:48:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.275

PFS not locked. Waiting for lock...

PFS took 0.800000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:15

Channel:Brightfield set at:11-Jul-2019 18:48:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 18:48:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:15

Channel configuration set to: GFP: 11-Jul-2019 18:48:16

Channel:GFP set at:11-Jul-2019 18:48:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 18:48:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:20

Channel:Brightfield set at:11-Jul-2019 18:48:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 18:48:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:20

Channel configuration set to: GFP: 11-Jul-2019 18:48:21

Channel:GFP set at:11-Jul-2019 18:48:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 18:48:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:24

Channel:Brightfield set at:11-Jul-2019 18:48:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 11-Jul-2019 18:48:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:25

Channel configuration set to: GFP: 11-Jul-2019 18:48:26

Channel:GFP set at:11-Jul-2019 18:48:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 11-Jul-2019 18:48:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:29

Channel:Brightfield set at:11-Jul-2019 18:48:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 11-Jul-2019 18:48:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:30

Channel configuration set to: GFP: 11-Jul-2019 18:48:31

Channel:GFP set at:11-Jul-2019 18:48:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 11-Jul-2019 18:48:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:34

Channel:Brightfield set at:11-Jul-2019 18:48:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 18:48:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:35

Channel configuration set to: GFP: 11-Jul-2019 18:48:36

Channel:GFP set at:11-Jul-2019 18:48:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 18:48:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:39

Channel:Brightfield set at:11-Jul-2019 18:48:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 18:48:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:40

Channel configuration set to: GFP: 11-Jul-2019 18:48:41

Channel:GFP set at:11-Jul-2019 18:48:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 18:48:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:44

Channel:Brightfield set at:11-Jul-2019 18:48:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 18:48:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:44

Channel configuration set to: GFP: 11-Jul-2019 18:48:45

Channel:GFP set at:11-Jul-2019 18:48:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 18:48:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:48

Channel:Brightfield set at:11-Jul-2019 18:48:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 18:48:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:48

Channel configuration set to: GFP: 11-Jul-2019 18:48:50

Channel:GFP set at:11-Jul-2019 18:48:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 18:48:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:50

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.575

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:53

Channel:Brightfield set at:11-Jul-2019 18:48:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1001. 11-Jul-2019 18:48:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:54

Channel configuration set to: GFP: 11-Jul-2019 18:48:55

Channel:GFP set at:11-Jul-2019 18:48:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1251. 11-Jul-2019 18:48:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:55

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-4.125

Channel configuration set to: Brightfield: 11-Jul-2019 18:48:58

Channel:Brightfield set at:11-Jul-2019 18:48:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 11-Jul-2019 18:48:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:48:58

Channel configuration set to: GFP: 11-Jul-2019 18:49:00

Channel:GFP set at:11-Jul-2019 18:49:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 18:49:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:49:00

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:29 completed at:11-Jul-2019 18:49:02

Time since start of timelapse:8488.5234

PFS is locked

Time to next time point:211.5357



------Time point_30------

Memory available for all arrays: 61068197888

Memory used by Matlab: 2206556160

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:52:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.2

Channel configuration set to: Brightfield: 11-Jul-2019 18:52:35

Channel:Brightfield set at:11-Jul-2019 18:52:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 18:52:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:52:35

Channel configuration set to: GFP: 11-Jul-2019 18:52:37

Channel:GFP set at:11-Jul-2019 18:52:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 18:52:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:52:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:52:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.75

Channel configuration set to: Brightfield: 11-Jul-2019 18:52:41

Channel:Brightfield set at:11-Jul-2019 18:52:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 18:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:52:41

Channel configuration set to: GFP: 11-Jul-2019 18:52:42

Channel:GFP set at:11-Jul-2019 18:52:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 18:52:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:52:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:52:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.925

Channel configuration set to: Brightfield: 11-Jul-2019 18:52:46

Channel:Brightfield set at:11-Jul-2019 18:52:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 18:52:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:52:46

Channel configuration set to: GFP: 11-Jul-2019 18:52:47

Channel:GFP set at:11-Jul-2019 18:52:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 18:52:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:52:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:52:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 18:52:51

Channel:Brightfield set at:11-Jul-2019 18:52:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3751. 11-Jul-2019 18:52:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:52:51

Channel configuration set to: GFP: 11-Jul-2019 18:52:52

Channel:GFP set at:11-Jul-2019 18:52:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 11-Jul-2019 18:52:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:52:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:52:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 18:52:55

Channel:Brightfield set at:11-Jul-2019 18:52:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 18:52:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:52:56

Channel configuration set to: GFP: 11-Jul-2019 18:52:57

Channel:GFP set at:11-Jul-2019 18:52:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 11-Jul-2019 18:52:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:52:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:52:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.65

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:01

Channel:Brightfield set at:11-Jul-2019 18:53:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8751. 11-Jul-2019 18:53:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:01

Channel configuration set to: GFP: 11-Jul-2019 18:53:02

Channel:GFP set at:11-Jul-2019 18:53:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8751. 11-Jul-2019 18:53:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:05

Channel:Brightfield set at:11-Jul-2019 18:53:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 18:53:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:05

Channel configuration set to: GFP: 11-Jul-2019 18:53:07

Channel:GFP set at:11-Jul-2019 18:53:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 18:53:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:10

Channel:Brightfield set at:11-Jul-2019 18:53:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 18:53:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:10

Channel configuration set to: GFP: 11-Jul-2019 18:53:12

Channel:GFP set at:11-Jul-2019 18:53:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 18:53:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.5

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:15

Channel:Brightfield set at:11-Jul-2019 18:53:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 11-Jul-2019 18:53:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:15

Channel configuration set to: GFP: 11-Jul-2019 18:53:16

Channel:GFP set at:11-Jul-2019 18:53:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 11-Jul-2019 18:53:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:20

Channel:Brightfield set at:11-Jul-2019 18:53:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 18:53:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:20

Channel configuration set to: GFP: 11-Jul-2019 18:53:21

Channel:GFP set at:11-Jul-2019 18:53:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 18:53:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:24

Channel:Brightfield set at:11-Jul-2019 18:53:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 18:53:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:25

Channel configuration set to: GFP: 11-Jul-2019 18:53:26

Channel:GFP set at:11-Jul-2019 18:53:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 18:53:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:29

Channel:Brightfield set at:11-Jul-2019 18:53:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 18:53:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:30

Channel configuration set to: GFP: 11-Jul-2019 18:53:31

Channel:GFP set at:11-Jul-2019 18:53:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 18:53:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:35

Channel:Brightfield set at:11-Jul-2019 18:53:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 18:53:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:35

Channel configuration set to: GFP: 11-Jul-2019 18:53:36

Channel:GFP set at:11-Jul-2019 18:53:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 18:53:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:40

Channel:Brightfield set at:11-Jul-2019 18:53:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 18:53:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:40

Channel configuration set to: GFP: 11-Jul-2019 18:53:41

Channel:GFP set at:11-Jul-2019 18:53:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 18:53:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:44

Channel:Brightfield set at:11-Jul-2019 18:53:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 18:53:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:44

Channel configuration set to: GFP: 11-Jul-2019 18:53:46

Channel:GFP set at:11-Jul-2019 18:53:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 18:53:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:49

Channel:Brightfield set at:11-Jul-2019 18:53:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 18:53:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:49

Channel configuration set to: GFP: 11-Jul-2019 18:53:50

Channel:GFP set at:11-Jul-2019 18:53:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 18:53:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.55

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:54

Channel:Brightfield set at:11-Jul-2019 18:53:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1251. 11-Jul-2019 18:53:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:54

Channel configuration set to: GFP: 11-Jul-2019 18:53:55

Channel:GFP set at:11-Jul-2019 18:53:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1501. 11-Jul-2019 18:53:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 18:53:59

Channel:Brightfield set at:11-Jul-2019 18:53:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 18:53:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:53:59

Channel configuration set to: GFP: 11-Jul-2019 18:54:00

Channel:GFP set at:11-Jul-2019 18:54:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 18:54:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:54:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:30 completed at:11-Jul-2019 18:54:03

Time since start of timelapse:8789.4361

PFS is locked

Time to next time point:210.6246



------Time point_31------

Memory available for all arrays: 61074776064

Memory used by Matlab: 2206556160

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.225

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:35

Channel:Brightfield set at:11-Jul-2019 18:57:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0751. 11-Jul-2019 18:57:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:57:35

Channel configuration set to: GFP: 11-Jul-2019 18:57:37

Channel:GFP set at:11-Jul-2019 18:57:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0751. 11-Jul-2019 18:57:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:57:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.775

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:40

Channel:Brightfield set at:11-Jul-2019 18:57:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 18:57:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:57:40

Channel configuration set to: GFP: 11-Jul-2019 18:57:42

Channel:GFP set at:11-Jul-2019 18:57:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 18:57:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:57:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.825

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:44

Channel:Brightfield set at:11-Jul-2019 18:57:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3751. 11-Jul-2019 18:57:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:57:45

Channel configuration set to: GFP: 11-Jul-2019 18:57:46

Channel:GFP set at:11-Jul-2019 18:57:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3751. 11-Jul-2019 18:57:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:57:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:49

Channel:Brightfield set at:11-Jul-2019 18:57:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 18:57:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:57:49

Channel configuration set to: GFP: 11-Jul-2019 18:57:51

Channel:GFP set at:11-Jul-2019 18:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 18:57:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:57:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.95

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:54

Channel:Brightfield set at:11-Jul-2019 18:57:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 18:57:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:57:54

Channel configuration set to: GFP: 11-Jul-2019 18:57:56

Channel:GFP set at:11-Jul-2019 18:57:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 18:57:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:57:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.725

Channel configuration set to: Brightfield: 11-Jul-2019 18:57:59

Channel:Brightfield set at:11-Jul-2019 18:57:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8751. 11-Jul-2019 18:57:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:57:59

Channel configuration set to: GFP: 11-Jul-2019 18:58:01

Channel:GFP set at:11-Jul-2019 18:58:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8751. 11-Jul-2019 18:58:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:04

Channel:Brightfield set at:11-Jul-2019 18:58:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 18:58:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:05

Channel configuration set to: GFP: 11-Jul-2019 18:58:06

Channel:GFP set at:11-Jul-2019 18:58:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 18:58:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:10

Channel:Brightfield set at:11-Jul-2019 18:58:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 18:58:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:10

Channel configuration set to: GFP: 11-Jul-2019 18:58:11

Channel:GFP set at:11-Jul-2019 18:58:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 18:58:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:15

Channel:Brightfield set at:11-Jul-2019 18:58:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 18:58:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:15

Channel configuration set to: GFP: 11-Jul-2019 18:58:17

Channel:GFP set at:11-Jul-2019 18:58:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 11-Jul-2019 18:58:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:20

Channel:Brightfield set at:11-Jul-2019 18:58:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 18:58:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:20

Channel configuration set to: GFP: 11-Jul-2019 18:58:21

Channel:GFP set at:11-Jul-2019 18:58:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4501. 11-Jul-2019 18:58:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:25

Channel:Brightfield set at:11-Jul-2019 18:58:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 18:58:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:25

Channel configuration set to: GFP: 11-Jul-2019 18:58:26

Channel:GFP set at:11-Jul-2019 18:58:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 18:58:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:29

Channel:Brightfield set at:11-Jul-2019 18:58:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 11-Jul-2019 18:58:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:29

Channel configuration set to: GFP: 11-Jul-2019 18:58:31

Channel:GFP set at:11-Jul-2019 18:58:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 11-Jul-2019 18:58:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:35

Channel:Brightfield set at:11-Jul-2019 18:58:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 18:58:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:35

Channel configuration set to: GFP: 11-Jul-2019 18:58:36

Channel:GFP set at:11-Jul-2019 18:58:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 18:58:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:39

Channel:Brightfield set at:11-Jul-2019 18:58:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 18:58:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:39

Channel configuration set to: GFP: 11-Jul-2019 18:58:41

Channel:GFP set at:11-Jul-2019 18:58:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 18:58:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:44

Channel:Brightfield set at:11-Jul-2019 18:58:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 18:58:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:44

Channel configuration set to: GFP: 11-Jul-2019 18:58:45

Channel:GFP set at:11-Jul-2019 18:58:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 18:58:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.125

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:49

Channel:Brightfield set at:11-Jul-2019 18:58:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1751. 11-Jul-2019 18:58:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:49

Channel configuration set to: GFP: 11-Jul-2019 18:58:50

Channel:GFP set at:11-Jul-2019 18:58:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 18:58:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.55

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:53

Channel:Brightfield set at:11-Jul-2019 18:58:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 18:58:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:54

Channel configuration set to: GFP: 11-Jul-2019 18:58:55

Channel:GFP set at:11-Jul-2019 18:58:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 18:58:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:55

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 18:58:58

Channel:Brightfield set at:11-Jul-2019 18:58:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 18:58:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:58:59

Channel configuration set to: GFP: 11-Jul-2019 18:59:00

Channel:GFP set at:11-Jul-2019 18:59:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 18:59:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 18:59:00

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:31 completed at:11-Jul-2019 18:59:02

Time since start of timelapse:9088.819

PFS is locked

Time to next time point:211.2432



------Time point_32------

Memory available for all arrays: 61078454272

Memory used by Matlab: 2206556160

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:02:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.2

Channel configuration set to: Brightfield: 11-Jul-2019 19:02:35

Channel:Brightfield set at:11-Jul-2019 19:02:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 19:02:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:02:35

Channel configuration set to: GFP: 11-Jul-2019 19:02:37

Channel:GFP set at:11-Jul-2019 19:02:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 19:02:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:02:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:02:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.675

Channel configuration set to: Brightfield: 11-Jul-2019 19:02:40

Channel:Brightfield set at:11-Jul-2019 19:02:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 11-Jul-2019 19:02:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:02:40

Channel configuration set to: GFP: 11-Jul-2019 19:02:42

Channel:GFP set at:11-Jul-2019 19:02:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1751. 11-Jul-2019 19:02:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:02:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:02:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.1

Channel configuration set to: Brightfield: 11-Jul-2019 19:02:45

Channel:Brightfield set at:11-Jul-2019 19:02:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 19:02:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:02:45

Channel configuration set to: GFP: 11-Jul-2019 19:02:47

Channel:GFP set at:11-Jul-2019 19:02:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 19:02:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:02:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:02:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 11-Jul-2019 19:02:51

Channel:Brightfield set at:11-Jul-2019 19:02:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 19:02:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:02:51

Channel configuration set to: GFP: 11-Jul-2019 19:02:52

Channel:GFP set at:11-Jul-2019 19:02:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 19:02:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:02:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:02:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 19:02:55

Channel:Brightfield set at:11-Jul-2019 19:02:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 19:02:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:02:55

Channel configuration set to: GFP: 11-Jul-2019 19:02:57

Channel:GFP set at:11-Jul-2019 19:02:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 19:02:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:02:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:02:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.925

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:00

Channel:Brightfield set at:11-Jul-2019 19:03:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 19:03:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:00

Channel configuration set to: GFP: 11-Jul-2019 19:03:01

Channel:GFP set at:11-Jul-2019 19:03:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 11-Jul-2019 19:03:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:05

Channel:Brightfield set at:11-Jul-2019 19:03:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 11-Jul-2019 19:03:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:05

Channel configuration set to: GFP: 11-Jul-2019 19:03:06

Channel:GFP set at:11-Jul-2019 19:03:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 19:03:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:10

Channel:Brightfield set at:11-Jul-2019 19:03:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 11-Jul-2019 19:03:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:11

Channel configuration set to: GFP: 11-Jul-2019 19:03:12

Channel:GFP set at:11-Jul-2019 19:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 11-Jul-2019 19:03:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:15

Channel:Brightfield set at:11-Jul-2019 19:03:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 11-Jul-2019 19:03:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:15

Channel configuration set to: GFP: 11-Jul-2019 19:03:16

Channel:GFP set at:11-Jul-2019 19:03:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 11-Jul-2019 19:03:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.7

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:19

Channel:Brightfield set at:11-Jul-2019 19:03:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 19:03:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:19

Channel configuration set to: GFP: 11-Jul-2019 19:03:21

Channel:GFP set at:11-Jul-2019 19:03:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 19:03:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:24

Channel:Brightfield set at:11-Jul-2019 19:03:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 19:03:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:25

Channel configuration set to: GFP: 11-Jul-2019 19:03:26

Channel:GFP set at:11-Jul-2019 19:03:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 19:03:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:30

Channel:Brightfield set at:11-Jul-2019 19:03:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 11-Jul-2019 19:03:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:30

Channel configuration set to: GFP: 11-Jul-2019 19:03:31

Channel:GFP set at:11-Jul-2019 19:03:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 11-Jul-2019 19:03:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.875

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:34

Channel:Brightfield set at:11-Jul-2019 19:03:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 11-Jul-2019 19:03:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:34

Channel configuration set to: GFP: 11-Jul-2019 19:03:36

Channel:GFP set at:11-Jul-2019 19:03:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 11-Jul-2019 19:03:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:39

Channel:Brightfield set at:11-Jul-2019 19:03:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 19:03:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:39

Channel configuration set to: GFP: 11-Jul-2019 19:03:41

Channel:GFP set at:11-Jul-2019 19:03:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 19:03:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:44

Channel:Brightfield set at:11-Jul-2019 19:03:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 11-Jul-2019 19:03:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:44

Channel configuration set to: GFP: 11-Jul-2019 19:03:45

Channel:GFP set at:11-Jul-2019 19:03:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 11-Jul-2019 19:03:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.1

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:49

Channel:Brightfield set at:11-Jul-2019 19:03:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 19:03:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:49

Channel configuration set to: GFP: 11-Jul-2019 19:03:50

Channel:GFP set at:11-Jul-2019 19:03:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 19:03:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:54

Channel:Brightfield set at:11-Jul-2019 19:03:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 19:03:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:54

Channel configuration set to: GFP: 11-Jul-2019 19:03:55

Channel:GFP set at:11-Jul-2019 19:03:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 19:03:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 11-Jul-2019 19:03:59

Channel:Brightfield set at:11-Jul-2019 19:03:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 19:03:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:03:59

Channel configuration set to: GFP: 11-Jul-2019 19:04:00

Channel:GFP set at:11-Jul-2019 19:04:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 19:04:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:04:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:32 completed at:11-Jul-2019 19:04:02

Time since start of timelapse:9388.9627

PFS is locked

Time to next time point:211.1008



------Time point_33------

Memory available for all arrays: 61081092096

Memory used by Matlab: 2206556160

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:07:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.3

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:07:36

Channel:Brightfield set at:11-Jul-2019 19:07:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 19:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:07:36

Channel configuration set to: GFP: 11-Jul-2019 19:07:37

Channel:GFP set at:11-Jul-2019 19:07:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 19:07:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:07:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:07:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:07:41

Channel:Brightfield set at:11-Jul-2019 19:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 11-Jul-2019 19:07:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:07:41

Channel configuration set to: GFP: 11-Jul-2019 19:07:42

Channel:GFP set at:11-Jul-2019 19:07:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 11-Jul-2019 19:07:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:07:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:07:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 11-Jul-2019 19:07:46

Channel:Brightfield set at:11-Jul-2019 19:07:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 19:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:07:46

Channel configuration set to: GFP: 11-Jul-2019 19:07:47

Channel:GFP set at:11-Jul-2019 19:07:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 19:07:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:07:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:07:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.4

Channel configuration set to: Brightfield: 11-Jul-2019 19:07:51

Channel:Brightfield set at:11-Jul-2019 19:07:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 11-Jul-2019 19:07:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:07:51

Channel configuration set to: GFP: 11-Jul-2019 19:07:52

Channel:GFP set at:11-Jul-2019 19:07:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3251. 11-Jul-2019 19:07:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:07:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:07:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 11-Jul-2019 19:07:56

Channel:Brightfield set at:11-Jul-2019 19:07:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 19:07:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:07:56

Channel configuration set to: GFP: 11-Jul-2019 19:07:58

Channel:GFP set at:11-Jul-2019 19:07:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 19:07:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:07:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:00

Channel:Brightfield set at:11-Jul-2019 19:08:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 19:08:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:01

Channel configuration set to: GFP: 11-Jul-2019 19:08:02

Channel:GFP set at:11-Jul-2019 19:08:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 19:08:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:06

Channel:Brightfield set at:11-Jul-2019 19:08:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 19:08:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:06

Channel configuration set to: GFP: 11-Jul-2019 19:08:08

Channel:GFP set at:11-Jul-2019 19:08:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 19:08:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:11

Channel:Brightfield set at:11-Jul-2019 19:08:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 19:08:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:12

Channel configuration set to: GFP: 11-Jul-2019 19:08:13

Channel:GFP set at:11-Jul-2019 19:08:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 19:08:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.4

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:17

Channel:Brightfield set at:11-Jul-2019 19:08:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 19:08:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:17

Channel configuration set to: GFP: 11-Jul-2019 19:08:18

Channel:GFP set at:11-Jul-2019 19:08:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 19:08:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:21

Channel:Brightfield set at:11-Jul-2019 19:08:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 11-Jul-2019 19:08:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:22

Channel configuration set to: GFP: 11-Jul-2019 19:08:23

Channel:GFP set at:11-Jul-2019 19:08:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3501. 11-Jul-2019 19:08:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:26

Channel:Brightfield set at:11-Jul-2019 19:08:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 19:08:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:27

Channel configuration set to: GFP: 11-Jul-2019 19:08:28

Channel:GFP set at:11-Jul-2019 19:08:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 11-Jul-2019 19:08:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:31

Channel:Brightfield set at:11-Jul-2019 19:08:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 19:08:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:32

Channel configuration set to: GFP: 11-Jul-2019 19:08:33

Channel:GFP set at:11-Jul-2019 19:08:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 19:08:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:37

Channel:Brightfield set at:11-Jul-2019 19:08:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 19:08:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:37

Channel configuration set to: GFP: 11-Jul-2019 19:08:38

Channel:GFP set at:11-Jul-2019 19:08:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 19:08:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:41

Channel:Brightfield set at:11-Jul-2019 19:08:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 19:08:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:41

Channel configuration set to: GFP: 11-Jul-2019 19:08:43

Channel:GFP set at:11-Jul-2019 19:08:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 19:08:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.3

PFS not locked. Waiting for lock...

PFS took 0.300000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:46

Channel:Brightfield set at:11-Jul-2019 19:08:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 19:08:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:46

Channel configuration set to: GFP: 11-Jul-2019 19:08:48

Channel:GFP set at:11-Jul-2019 19:08:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 19:08:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:48

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.125

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:51

Channel:Brightfield set at:11-Jul-2019 19:08:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 19:08:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:51

Channel configuration set to: GFP: 11-Jul-2019 19:08:53

Channel:GFP set at:11-Jul-2019 19:08:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 19:08:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:53

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.6

Channel configuration set to: Brightfield: 11-Jul-2019 19:08:55

Channel:Brightfield set at:11-Jul-2019 19:08:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 19:08:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:56

Channel configuration set to: GFP: 11-Jul-2019 19:08:57

Channel:GFP set at:11-Jul-2019 19:08:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 19:08:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:08:58

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:09:00

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 19:09:01

Channel:Brightfield set at:11-Jul-2019 19:09:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0501. 11-Jul-2019 19:09:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:09:01

Channel configuration set to: GFP: 11-Jul-2019 19:09:03

Channel:GFP set at:11-Jul-2019 19:09:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 19:09:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:09:03

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:33 completed at:11-Jul-2019 19:09:05

Time since start of timelapse:9691.4194

PFS is locked

Time to next time point:208.6459



------Time point_34------

Memory available for all arrays: 61088055296

Memory used by Matlab: 2206556160

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:12:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 11-Jul-2019 19:12:35

Channel:Brightfield set at:11-Jul-2019 19:12:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 19:12:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:12:35

Channel configuration set to: GFP: 11-Jul-2019 19:12:37

Channel:GFP set at:11-Jul-2019 19:12:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 19:12:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:12:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:12:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9

Channel configuration set to: Brightfield: 11-Jul-2019 19:12:40

Channel:Brightfield set at:11-Jul-2019 19:12:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 19:12:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:12:41

Channel configuration set to: GFP: 11-Jul-2019 19:12:42

Channel:GFP set at:11-Jul-2019 19:12:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 19:12:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:12:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:12:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 11-Jul-2019 19:12:45

Channel:Brightfield set at:11-Jul-2019 19:12:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1751. 11-Jul-2019 19:12:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:12:45

Channel configuration set to: GFP: 11-Jul-2019 19:12:46

Channel:GFP set at:11-Jul-2019 19:12:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1751. 11-Jul-2019 19:12:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:12:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:12:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.65

Channel configuration set to: Brightfield: 11-Jul-2019 19:12:50

Channel:Brightfield set at:11-Jul-2019 19:12:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 19:12:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:12:50

Channel configuration set to: GFP: 11-Jul-2019 19:12:51

Channel:GFP set at:11-Jul-2019 19:12:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 19:12:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:12:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:12:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 11-Jul-2019 19:12:55

Channel:Brightfield set at:11-Jul-2019 19:12:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 19:12:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:12:55

Channel configuration set to: GFP: 11-Jul-2019 19:12:57

Channel:GFP set at:11-Jul-2019 19:12:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7001. 11-Jul-2019 19:12:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:12:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:12:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:00

Channel:Brightfield set at:11-Jul-2019 19:13:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 19:13:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:00

Channel configuration set to: GFP: 11-Jul-2019 19:13:02

Channel:GFP set at:11-Jul-2019 19:13:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 19:13:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.125

PFS not locked. Waiting for lock...

PFS took 0.200000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:05

Channel:Brightfield set at:11-Jul-2019 19:13:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 19:13:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:05

Channel configuration set to: GFP: 11-Jul-2019 19:13:07

Channel:GFP set at:11-Jul-2019 19:13:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 19:13:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.425

PFS not locked. Waiting for lock...

PFS took 0.800000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:10

Channel:Brightfield set at:11-Jul-2019 19:13:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 19:13:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:11

Channel configuration set to: GFP: 11-Jul-2019 19:13:12

Channel:GFP set at:11-Jul-2019 19:13:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 19:13:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:15

Channel:Brightfield set at:11-Jul-2019 19:13:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 19:13:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:15

Channel configuration set to: GFP: 11-Jul-2019 19:13:16

Channel:GFP set at:11-Jul-2019 19:13:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 19:13:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:19

Channel:Brightfield set at:11-Jul-2019 19:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 19:13:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:19

Channel configuration set to: GFP: 11-Jul-2019 19:13:21

Channel:GFP set at:11-Jul-2019 19:13:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 19:13:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:24

Channel:Brightfield set at:11-Jul-2019 19:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 11-Jul-2019 19:13:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:24

Channel configuration set to: GFP: 11-Jul-2019 19:13:25

Channel:GFP set at:11-Jul-2019 19:13:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 11-Jul-2019 19:13:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:27

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:28

Channel:Brightfield set at:11-Jul-2019 19:13:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0001. 11-Jul-2019 19:13:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:29

Channel configuration set to: GFP: 11-Jul-2019 19:13:30

Channel:GFP set at:11-Jul-2019 19:13:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 11-Jul-2019 19:13:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:30

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:32

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:34

Channel:Brightfield set at:11-Jul-2019 19:13:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 19:13:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:34

Channel configuration set to: GFP: 11-Jul-2019 19:13:36

Channel:GFP set at:11-Jul-2019 19:13:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 19:13:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:39

Channel:Brightfield set at:11-Jul-2019 19:13:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 19:13:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:39

Channel configuration set to: GFP: 11-Jul-2019 19:13:40

Channel:GFP set at:11-Jul-2019 19:13:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 19:13:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:42

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:43

Channel:Brightfield set at:11-Jul-2019 19:13:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 19:13:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:43

Channel configuration set to: GFP: 11-Jul-2019 19:13:45

Channel:GFP set at:11-Jul-2019 19:13:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 19:13:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:45

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:47

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.475

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:48

Channel:Brightfield set at:11-Jul-2019 19:13:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 19:13:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:49

Channel configuration set to: GFP: 11-Jul-2019 19:13:50

Channel:GFP set at:11-Jul-2019 19:13:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 19:13:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:50

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:52

Channel:Brightfield set at:11-Jul-2019 19:13:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 11-Jul-2019 19:13:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:53

Channel configuration set to: GFP: 11-Jul-2019 19:13:54

Channel:GFP set at:11-Jul-2019 19:13:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 11-Jul-2019 19:13:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:55

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:56

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 11-Jul-2019 19:13:58

Channel:Brightfield set at:11-Jul-2019 19:13:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 19:13:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:13:58

Channel configuration set to: GFP: 11-Jul-2019 19:14:00

Channel:GFP set at:11-Jul-2019 19:14:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 19:14:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:14:00

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:34 completed at:11-Jul-2019 19:14:02

Time since start of timelapse:9988.4644

PFS is locked

Time to next time point:211.6023



------Time point_35------

Memory available for all arrays: 61096767488

Memory used by Matlab: 2206556160

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:17:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.6

Channel configuration set to: Brightfield: 11-Jul-2019 19:17:35

Channel:Brightfield set at:11-Jul-2019 19:17:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 19:17:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:17:35

Channel configuration set to: GFP: 11-Jul-2019 19:17:37

Channel:GFP set at:11-Jul-2019 19:17:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 19:17:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:17:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:17:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.05

Channel configuration set to: Brightfield: 11-Jul-2019 19:17:40

Channel:Brightfield set at:11-Jul-2019 19:17:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 19:17:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:17:40

Channel configuration set to: GFP: 11-Jul-2019 19:17:42

Channel:GFP set at:11-Jul-2019 19:17:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 19:17:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:17:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:17:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.4

Channel configuration set to: Brightfield: 11-Jul-2019 19:17:45

Channel:Brightfield set at:11-Jul-2019 19:17:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 19:17:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:17:45

Channel configuration set to: GFP: 11-Jul-2019 19:17:47

Channel:GFP set at:11-Jul-2019 19:17:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 19:17:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:17:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:17:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.65

Channel configuration set to: Brightfield: 11-Jul-2019 19:17:50

Channel:Brightfield set at:11-Jul-2019 19:17:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 19:17:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:17:50

Channel configuration set to: GFP: 11-Jul-2019 19:17:52

Channel:GFP set at:11-Jul-2019 19:17:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 19:17:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:17:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:17:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 11-Jul-2019 19:17:55

Channel:Brightfield set at:11-Jul-2019 19:17:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 19:17:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:17:56

Channel configuration set to: GFP: 11-Jul-2019 19:17:57

Channel:GFP set at:11-Jul-2019 19:17:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 19:17:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:17:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:17:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.925

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:00

Channel:Brightfield set at:11-Jul-2019 19:18:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 19:18:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:00

Channel configuration set to: GFP: 11-Jul-2019 19:18:02

Channel:GFP set at:11-Jul-2019 19:18:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 19:18:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:05

Channel:Brightfield set at:11-Jul-2019 19:18:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 19:18:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:06

Channel configuration set to: GFP: 11-Jul-2019 19:18:07

Channel:GFP set at:11-Jul-2019 19:18:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 19:18:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:11

Channel:Brightfield set at:11-Jul-2019 19:18:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 19:18:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:11

Channel configuration set to: GFP: 11-Jul-2019 19:18:12

Channel:GFP set at:11-Jul-2019 19:18:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 19:18:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.625

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:15

Channel:Brightfield set at:11-Jul-2019 19:18:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 19:18:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:15

Channel configuration set to: GFP: 11-Jul-2019 19:18:17

Channel:GFP set at:11-Jul-2019 19:18:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 19:18:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:19

Channel:Brightfield set at:11-Jul-2019 19:18:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 11-Jul-2019 19:18:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:20

Channel configuration set to: GFP: 11-Jul-2019 19:18:21

Channel:GFP set at:11-Jul-2019 19:18:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 11-Jul-2019 19:18:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.25

PFS not locked. Waiting for lock...

PFS took 0.200000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:24

Channel:Brightfield set at:11-Jul-2019 19:18:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 11-Jul-2019 19:18:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:25

Channel configuration set to: GFP: 11-Jul-2019 19:18:26

Channel:GFP set at:11-Jul-2019 19:18:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 11-Jul-2019 19:18:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:30

Channel:Brightfield set at:11-Jul-2019 19:18:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 11-Jul-2019 19:18:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:30

Channel configuration set to: GFP: 11-Jul-2019 19:18:31

Channel:GFP set at:11-Jul-2019 19:18:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 11-Jul-2019 19:18:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.25

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:35

Channel:Brightfield set at:11-Jul-2019 19:18:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 19:18:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:35

Channel configuration set to: GFP: 11-Jul-2019 19:18:37

Channel:GFP set at:11-Jul-2019 19:18:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 19:18:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:40

Channel:Brightfield set at:11-Jul-2019 19:18:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 19:18:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:40

Channel configuration set to: GFP: 11-Jul-2019 19:18:41

Channel:GFP set at:11-Jul-2019 19:18:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 19:18:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:45

Channel:Brightfield set at:11-Jul-2019 19:18:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 19:18:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:45

Channel configuration set to: GFP: 11-Jul-2019 19:18:46

Channel:GFP set at:11-Jul-2019 19:18:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 19:18:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:49

Channel:Brightfield set at:11-Jul-2019 19:18:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 19:18:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:50

Channel configuration set to: GFP: 11-Jul-2019 19:18:51

Channel:GFP set at:11-Jul-2019 19:18:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 19:18:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.925

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:54

Channel:Brightfield set at:11-Jul-2019 19:18:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 19:18:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:55

Channel configuration set to: GFP: 11-Jul-2019 19:18:56

Channel:GFP set at:11-Jul-2019 19:18:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 19:18:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-4.45

Channel configuration set to: Brightfield: 11-Jul-2019 19:18:59

Channel:Brightfield set at:11-Jul-2019 19:18:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 11-Jul-2019 19:18:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:18:59

Channel configuration set to: GFP: 11-Jul-2019 19:19:00

Channel:GFP set at:11-Jul-2019 19:19:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7501. 11-Jul-2019 19:19:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:19:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:35 completed at:11-Jul-2019 19:19:03

Time since start of timelapse:10289.5575

PFS is locked

Time to next time point:210.5107



------Time point_36------

Memory available for all arrays: 61094141952

Memory used by Matlab: 2206687232

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:22:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 11-Jul-2019 19:22:35

Channel:Brightfield set at:11-Jul-2019 19:22:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 19:22:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:22:35

Channel configuration set to: GFP: 11-Jul-2019 19:22:37

Channel:GFP set at:11-Jul-2019 19:22:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 19:22:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:22:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:22:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.775

Channel configuration set to: Brightfield: 11-Jul-2019 19:22:40

Channel:Brightfield set at:11-Jul-2019 19:22:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0751. 11-Jul-2019 19:22:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:22:40

Channel configuration set to: GFP: 11-Jul-2019 19:22:42

Channel:GFP set at:11-Jul-2019 19:22:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 19:22:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:22:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:22:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.175

Channel configuration set to: Brightfield: 11-Jul-2019 19:22:45

Channel:Brightfield set at:11-Jul-2019 19:22:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 19:22:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:22:45

Channel configuration set to: GFP: 11-Jul-2019 19:22:47

Channel:GFP set at:11-Jul-2019 19:22:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 19:22:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:22:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:22:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.425

Channel configuration set to: Brightfield: 11-Jul-2019 19:22:50

Channel:Brightfield set at:11-Jul-2019 19:22:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 11-Jul-2019 19:22:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:22:50

Channel configuration set to: GFP: 11-Jul-2019 19:22:51

Channel:GFP set at:11-Jul-2019 19:22:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 11-Jul-2019 19:22:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:22:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:22:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 11-Jul-2019 19:22:55

Channel:Brightfield set at:11-Jul-2019 19:22:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 19:22:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:22:55

Channel configuration set to: GFP: 11-Jul-2019 19:22:56

Channel:GFP set at:11-Jul-2019 19:22:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 19:22:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:22:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:22:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.775

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:00

Channel:Brightfield set at:11-Jul-2019 19:23:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 19:23:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:00

Channel configuration set to: GFP: 11-Jul-2019 19:23:02

Channel:GFP set at:11-Jul-2019 19:23:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 19:23:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:05

Channel:Brightfield set at:11-Jul-2019 19:23:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 19:23:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:06

Channel configuration set to: GFP: 11-Jul-2019 19:23:07

Channel:GFP set at:11-Jul-2019 19:23:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 19:23:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.7

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:10

Channel:Brightfield set at:11-Jul-2019 19:23:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 19:23:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:10

Channel configuration set to: GFP: 11-Jul-2019 19:23:12

Channel:GFP set at:11-Jul-2019 19:23:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1751. 11-Jul-2019 19:23:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.4

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:15

Channel:Brightfield set at:11-Jul-2019 19:23:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 19:23:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:16

Channel configuration set to: GFP: 11-Jul-2019 19:23:17

Channel:GFP set at:11-Jul-2019 19:23:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 19:23:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:20

Channel:Brightfield set at:11-Jul-2019 19:23:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 11-Jul-2019 19:23:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:21

Channel configuration set to: GFP: 11-Jul-2019 19:23:22

Channel:GFP set at:11-Jul-2019 19:23:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 11-Jul-2019 19:23:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:25

Channel:Brightfield set at:11-Jul-2019 19:23:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 19:23:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:25

Channel configuration set to: GFP: 11-Jul-2019 19:23:26

Channel:GFP set at:11-Jul-2019 19:23:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 19:23:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:30

Channel:Brightfield set at:11-Jul-2019 19:23:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 19:23:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:30

Channel configuration set to: GFP: 11-Jul-2019 19:23:31

Channel:GFP set at:11-Jul-2019 19:23:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 19:23:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.125

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:34

Channel:Brightfield set at:11-Jul-2019 19:23:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 19:23:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:35

Channel configuration set to: GFP: 11-Jul-2019 19:23:36

Channel:GFP set at:11-Jul-2019 19:23:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 19:23:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:39

Channel:Brightfield set at:11-Jul-2019 19:23:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 19:23:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:39

Channel configuration set to: GFP: 11-Jul-2019 19:23:41

Channel:GFP set at:11-Jul-2019 19:23:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 19:23:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:44

Channel:Brightfield set at:11-Jul-2019 19:23:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 19:23:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:44

Channel configuration set to: GFP: 11-Jul-2019 19:23:45

Channel:GFP set at:11-Jul-2019 19:23:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 19:23:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.2

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:49

Channel:Brightfield set at:11-Jul-2019 19:23:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 19:23:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:50

Channel configuration set to: GFP: 11-Jul-2019 19:23:51

Channel:GFP set at:11-Jul-2019 19:23:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 19:23:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.775

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:54

Channel:Brightfield set at:11-Jul-2019 19:23:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 11-Jul-2019 19:23:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:54

Channel configuration set to: GFP: 11-Jul-2019 19:23:56

Channel:GFP set at:11-Jul-2019 19:23:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 11-Jul-2019 19:23:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 19:23:59

Channel:Brightfield set at:11-Jul-2019 19:23:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 19:23:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:23:59

Channel configuration set to: GFP: 11-Jul-2019 19:24:01

Channel:GFP set at:11-Jul-2019 19:24:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 19:24:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:24:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:36 completed at:11-Jul-2019 19:24:03

Time since start of timelapse:10589.4466

PFS is locked

Time to next time point:210.6231



------Time point_37------

Memory available for all arrays: 61091213312

Memory used by Matlab: 2206687232

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:27:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 11-Jul-2019 19:27:35

Channel:Brightfield set at:11-Jul-2019 19:27:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9501. 11-Jul-2019 19:27:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:27:35

Channel configuration set to: GFP: 11-Jul-2019 19:27:37

Channel:GFP set at:11-Jul-2019 19:27:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 19:27:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:27:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:27:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.825

Channel configuration set to: Brightfield: 11-Jul-2019 19:27:40

Channel:Brightfield set at:11-Jul-2019 19:27:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 11-Jul-2019 19:27:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:27:40

Channel configuration set to: GFP: 11-Jul-2019 19:27:42

Channel:GFP set at:11-Jul-2019 19:27:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 19:27:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:27:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:27:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.825

Channel configuration set to: Brightfield: 11-Jul-2019 19:27:44

Channel:Brightfield set at:11-Jul-2019 19:27:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2751. 11-Jul-2019 19:27:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:27:45

Channel configuration set to: GFP: 11-Jul-2019 19:27:46

Channel:GFP set at:11-Jul-2019 19:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3001. 11-Jul-2019 19:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:27:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:27:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 11-Jul-2019 19:27:49

Channel:Brightfield set at:11-Jul-2019 19:27:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3251. 11-Jul-2019 19:27:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:27:50

Channel configuration set to: GFP: 11-Jul-2019 19:27:51

Channel:GFP set at:11-Jul-2019 19:27:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 11-Jul-2019 19:27:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:27:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:27:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 11-Jul-2019 19:27:54

Channel:Brightfield set at:11-Jul-2019 19:27:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 19:27:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:27:55

Channel configuration set to: GFP: 11-Jul-2019 19:27:56

Channel:GFP set at:11-Jul-2019 19:27:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 19:27:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:27:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:27:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.8

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:00

Channel:Brightfield set at:11-Jul-2019 19:28:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 11-Jul-2019 19:28:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:00

Channel configuration set to: GFP: 11-Jul-2019 19:28:01

Channel:GFP set at:11-Jul-2019 19:28:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 19:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:05

Channel:Brightfield set at:11-Jul-2019 19:28:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 19:28:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:06

Channel configuration set to: GFP: 11-Jul-2019 19:28:07

Channel:GFP set at:11-Jul-2019 19:28:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 19:28:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:11

Channel:Brightfield set at:11-Jul-2019 19:28:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 19:28:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:11

Channel configuration set to: GFP: 11-Jul-2019 19:28:12

Channel:GFP set at:11-Jul-2019 19:28:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 19:28:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:16

Channel:Brightfield set at:11-Jul-2019 19:28:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 19:28:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:16

Channel configuration set to: GFP: 11-Jul-2019 19:28:17

Channel:GFP set at:11-Jul-2019 19:28:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 19:28:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:21

Channel:Brightfield set at:11-Jul-2019 19:28:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 11-Jul-2019 19:28:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:21

Channel configuration set to: GFP: 11-Jul-2019 19:28:22

Channel:GFP set at:11-Jul-2019 19:28:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 11-Jul-2019 19:28:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:25

Channel:Brightfield set at:11-Jul-2019 19:28:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 11-Jul-2019 19:28:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:26

Channel configuration set to: GFP: 11-Jul-2019 19:28:27

Channel:GFP set at:11-Jul-2019 19:28:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 11-Jul-2019 19:28:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:30

Channel:Brightfield set at:11-Jul-2019 19:28:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 19:28:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:31

Channel configuration set to: GFP: 11-Jul-2019 19:28:32

Channel:GFP set at:11-Jul-2019 19:28:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 19:28:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:36

Channel:Brightfield set at:11-Jul-2019 19:28:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 19:28:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:36

Channel configuration set to: GFP: 11-Jul-2019 19:28:37

Channel:GFP set at:11-Jul-2019 19:28:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 19:28:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:40

Channel:Brightfield set at:11-Jul-2019 19:28:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 19:28:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:41

Channel configuration set to: GFP: 11-Jul-2019 19:28:42

Channel:GFP set at:11-Jul-2019 19:28:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 19:28:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:46

Channel:Brightfield set at:11-Jul-2019 19:28:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 19:28:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:46

Channel configuration set to: GFP: 11-Jul-2019 19:28:47

Channel:GFP set at:11-Jul-2019 19:28:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 19:28:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:48

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.2

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:51

Channel:Brightfield set at:11-Jul-2019 19:28:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 19:28:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:51

Channel configuration set to: GFP: 11-Jul-2019 19:28:52

Channel:GFP set at:11-Jul-2019 19:28:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 11-Jul-2019 19:28:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:53

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:54

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:55

Channel:Brightfield set at:11-Jul-2019 19:28:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 19:28:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:55

Channel configuration set to: GFP: 11-Jul-2019 19:28:57

Channel:GFP set at:11-Jul-2019 19:28:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2751. 11-Jul-2019 19:28:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:28:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:28:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 11-Jul-2019 19:29:00

Channel:Brightfield set at:11-Jul-2019 19:29:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 19:29:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:29:01

Channel configuration set to: GFP: 11-Jul-2019 19:29:02

Channel:GFP set at:11-Jul-2019 19:29:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 19:29:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:29:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:37 completed at:11-Jul-2019 19:29:04

Time since start of timelapse:10890.885

PFS is locked

Time to next time point:209.1861



------Time point_38------

Memory available for all arrays: 61094576128

Memory used by Matlab: 2206687232

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.475

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:35

Channel:Brightfield set at:11-Jul-2019 19:32:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7751. 11-Jul-2019 19:32:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:32:35

Channel configuration set to: GFP: 11-Jul-2019 19:32:37

Channel:GFP set at:11-Jul-2019 19:32:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7751. 11-Jul-2019 19:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:32:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.975

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:40

Channel:Brightfield set at:11-Jul-2019 19:32:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 11-Jul-2019 19:32:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:32:40

Channel configuration set to: GFP: 11-Jul-2019 19:32:42

Channel:GFP set at:11-Jul-2019 19:32:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8751. 11-Jul-2019 19:32:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:32:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.375

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:45

Channel:Brightfield set at:11-Jul-2019 19:32:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 19:32:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:32:45

Channel configuration set to: GFP: 11-Jul-2019 19:32:47

Channel:GFP set at:11-Jul-2019 19:32:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 19:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:32:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.55

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:50

Channel:Brightfield set at:11-Jul-2019 19:32:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 19:32:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:32:50

Channel configuration set to: GFP: 11-Jul-2019 19:32:51

Channel:GFP set at:11-Jul-2019 19:32:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 19:32:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:32:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:55

Channel:Brightfield set at:11-Jul-2019 19:32:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 19:32:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:32:55

Channel configuration set to: GFP: 11-Jul-2019 19:32:56

Channel:GFP set at:11-Jul-2019 19:32:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 19:32:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:32:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.775

PFS not locked. Waiting for lock...

PFS took 0.050000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:32:59

Channel:Brightfield set at:11-Jul-2019 19:32:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 19:33:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:00

Channel configuration set to: GFP: 11-Jul-2019 19:33:01

Channel:GFP set at:11-Jul-2019 19:33:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 11-Jul-2019 19:33:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:05

Channel:Brightfield set at:11-Jul-2019 19:33:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 19:33:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:05

Channel configuration set to: GFP: 11-Jul-2019 19:33:06

Channel:GFP set at:11-Jul-2019 19:33:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 19:33:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:10

Channel:Brightfield set at:11-Jul-2019 19:33:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 19:33:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:10

Channel configuration set to: GFP: 11-Jul-2019 19:33:12

Channel:GFP set at:11-Jul-2019 19:33:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 19:33:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:15

Channel:Brightfield set at:11-Jul-2019 19:33:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 11-Jul-2019 19:33:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:16

Channel configuration set to: GFP: 11-Jul-2019 19:33:17

Channel:GFP set at:11-Jul-2019 19:33:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 11-Jul-2019 19:33:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:20

Channel:Brightfield set at:11-Jul-2019 19:33:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1751. 11-Jul-2019 19:33:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:20

Channel configuration set to: GFP: 11-Jul-2019 19:33:22

Channel:GFP set at:11-Jul-2019 19:33:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1751. 11-Jul-2019 19:33:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:25

Channel:Brightfield set at:11-Jul-2019 19:33:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 11-Jul-2019 19:33:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:25

Channel configuration set to: GFP: 11-Jul-2019 19:33:26

Channel:GFP set at:11-Jul-2019 19:33:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 11-Jul-2019 19:33:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:29

Channel:Brightfield set at:11-Jul-2019 19:33:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 11-Jul-2019 19:33:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:30

Channel configuration set to: GFP: 11-Jul-2019 19:33:31

Channel:GFP set at:11-Jul-2019 19:33:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 11-Jul-2019 19:33:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:35

Channel:Brightfield set at:11-Jul-2019 19:33:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 19:33:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:35

Channel configuration set to: GFP: 11-Jul-2019 19:33:37

Channel:GFP set at:11-Jul-2019 19:33:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 19:33:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:40

Channel:Brightfield set at:11-Jul-2019 19:33:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 19:33:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:40

Channel configuration set to: GFP: 11-Jul-2019 19:33:41

Channel:GFP set at:11-Jul-2019 19:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 19:33:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.425

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:44

Channel:Brightfield set at:11-Jul-2019 19:33:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 11-Jul-2019 19:33:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:45

Channel configuration set to: GFP: 11-Jul-2019 19:33:46

Channel:GFP set at:11-Jul-2019 19:33:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 11-Jul-2019 19:33:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.225

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:50

Channel:Brightfield set at:11-Jul-2019 19:33:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 19:33:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:50

Channel configuration set to: GFP: 11-Jul-2019 19:33:51

Channel:GFP set at:11-Jul-2019 19:33:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 19:33:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:54

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:54

Channel:Brightfield set at:11-Jul-2019 19:33:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 11-Jul-2019 19:33:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:55

Channel configuration set to: GFP: 11-Jul-2019 19:33:56

Channel:GFP set at:11-Jul-2019 19:33:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 11-Jul-2019 19:33:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:33:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:33:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 11-Jul-2019 19:34:00

Channel:Brightfield set at:11-Jul-2019 19:34:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 19:34:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:34:00

Channel configuration set to: GFP: 11-Jul-2019 19:34:02

Channel:GFP set at:11-Jul-2019 19:34:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 19:34:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:34:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:38 completed at:11-Jul-2019 19:34:03

Time since start of timelapse:11190.4018

PFS is locked

Time to next time point:209.6711



------Time point_39------

Memory available for all arrays: 61083865088

Memory used by Matlab: 2206687232

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:35

Channel:Brightfield set at:11-Jul-2019 19:37:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 19:37:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:37:35

Channel configuration set to: GFP: 11-Jul-2019 19:37:37

Channel:GFP set at:11-Jul-2019 19:37:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 11-Jul-2019 19:37:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:37:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:40

Channel:Brightfield set at:11-Jul-2019 19:37:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 11-Jul-2019 19:37:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:37:40

Channel configuration set to: GFP: 11-Jul-2019 19:37:42

Channel:GFP set at:11-Jul-2019 19:37:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 11-Jul-2019 19:37:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:37:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.875

PFS not locked. Waiting for lock...

PFS took 0.700000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:45

Channel:Brightfield set at:11-Jul-2019 19:37:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 19:37:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:37:45

Channel configuration set to: GFP: 11-Jul-2019 19:37:47

Channel:GFP set at:11-Jul-2019 19:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 19:37:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:37:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:50

Channel:Brightfield set at:11-Jul-2019 19:37:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 19:37:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:37:50

Channel configuration set to: GFP: 11-Jul-2019 19:37:51

Channel:GFP set at:11-Jul-2019 19:37:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 19:37:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:37:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:54

Channel:Brightfield set at:11-Jul-2019 19:37:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 19:37:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:37:55

Channel configuration set to: GFP: 11-Jul-2019 19:37:56

Channel:GFP set at:11-Jul-2019 19:37:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 19:37:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:37:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.025

Channel configuration set to: Brightfield: 11-Jul-2019 19:37:59

Channel:Brightfield set at:11-Jul-2019 19:37:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 19:37:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:37:59

Channel configuration set to: GFP: 11-Jul-2019 19:38:01

Channel:GFP set at:11-Jul-2019 19:38:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 19:38:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:04

Channel:Brightfield set at:11-Jul-2019 19:38:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 19:38:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:04

Channel configuration set to: GFP: 11-Jul-2019 19:38:06

Channel:GFP set at:11-Jul-2019 19:38:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 19:38:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:10

Channel:Brightfield set at:11-Jul-2019 19:38:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 19:38:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:10

Channel configuration set to: GFP: 11-Jul-2019 19:38:11

Channel:GFP set at:11-Jul-2019 19:38:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 19:38:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:15

Channel:Brightfield set at:11-Jul-2019 19:38:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 19:38:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:15

Channel configuration set to: GFP: 11-Jul-2019 19:38:16

Channel:GFP set at:11-Jul-2019 19:38:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 19:38:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:20

Channel:Brightfield set at:11-Jul-2019 19:38:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 11-Jul-2019 19:38:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:20

Channel configuration set to: GFP: 11-Jul-2019 19:38:22

Channel:GFP set at:11-Jul-2019 19:38:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 11-Jul-2019 19:38:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:25

Channel:Brightfield set at:11-Jul-2019 19:38:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 11-Jul-2019 19:38:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:25

Channel configuration set to: GFP: 11-Jul-2019 19:38:26

Channel:GFP set at:11-Jul-2019 19:38:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 11-Jul-2019 19:38:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:30

Channel:Brightfield set at:11-Jul-2019 19:38:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 11-Jul-2019 19:38:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:30

Channel configuration set to: GFP: 11-Jul-2019 19:38:32

Channel:GFP set at:11-Jul-2019 19:38:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 11-Jul-2019 19:38:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.875

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:34

Channel:Brightfield set at:11-Jul-2019 19:38:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 19:38:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:35

Channel configuration set to: GFP: 11-Jul-2019 19:38:36

Channel:GFP set at:11-Jul-2019 19:38:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 19:38:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:40

Channel:Brightfield set at:11-Jul-2019 19:38:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 19:38:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:40

Channel configuration set to: GFP: 11-Jul-2019 19:38:41

Channel:GFP set at:11-Jul-2019 19:38:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 19:38:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:45

Channel:Brightfield set at:11-Jul-2019 19:38:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 19:38:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:45

Channel configuration set to: GFP: 11-Jul-2019 19:38:46

Channel:GFP set at:11-Jul-2019 19:38:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 19:38:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.1

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:50

Channel:Brightfield set at:11-Jul-2019 19:38:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 19:38:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:50

Channel configuration set to: GFP: 11-Jul-2019 19:38:51

Channel:GFP set at:11-Jul-2019 19:38:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 19:38:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.65

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:55

Channel:Brightfield set at:11-Jul-2019 19:38:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 11-Jul-2019 19:38:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:55

Channel configuration set to: GFP: 11-Jul-2019 19:38:56

Channel:GFP set at:11-Jul-2019 19:38:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 11-Jul-2019 19:38:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:38:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:38:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 19:39:00

Channel:Brightfield set at:11-Jul-2019 19:39:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 19:39:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:39:00

Channel configuration set to: GFP: 11-Jul-2019 19:39:01

Channel:GFP set at:11-Jul-2019 19:39:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0751. 11-Jul-2019 19:39:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:39:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:39 completed at:11-Jul-2019 19:39:03

Time since start of timelapse:11490.084

PFS is locked

Time to next time point:209.9903



------Time point_40------

Memory available for all arrays: 61081468928

Memory used by Matlab: 2206818304

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:42:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 11-Jul-2019 19:42:35

Channel:Brightfield set at:11-Jul-2019 19:42:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0501. 11-Jul-2019 19:42:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:42:35

Channel configuration set to: GFP: 11-Jul-2019 19:42:37

Channel:GFP set at:11-Jul-2019 19:42:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0501. 11-Jul-2019 19:42:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:42:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:42:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.675

Channel configuration set to: Brightfield: 11-Jul-2019 19:42:40

Channel:Brightfield set at:11-Jul-2019 19:42:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 19:42:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:42:40

Channel configuration set to: GFP: 11-Jul-2019 19:42:42

Channel:GFP set at:11-Jul-2019 19:42:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1751. 11-Jul-2019 19:42:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:42:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:42:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.825

Channel configuration set to: Brightfield: 11-Jul-2019 19:42:44

Channel:Brightfield set at:11-Jul-2019 19:42:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5251. 11-Jul-2019 19:42:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:42:45

Channel configuration set to: GFP: 11-Jul-2019 19:42:46

Channel:GFP set at:11-Jul-2019 19:42:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5251. 11-Jul-2019 19:42:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:42:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:42:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 11-Jul-2019 19:42:50

Channel:Brightfield set at:11-Jul-2019 19:42:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 11-Jul-2019 19:42:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:42:50

Channel configuration set to: GFP: 11-Jul-2019 19:42:51

Channel:GFP set at:11-Jul-2019 19:42:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 11-Jul-2019 19:42:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:42:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:42:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.95

Channel configuration set to: Brightfield: 11-Jul-2019 19:42:55

Channel:Brightfield set at:11-Jul-2019 19:42:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 19:42:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:42:55

Channel configuration set to: GFP: 11-Jul-2019 19:42:56

Channel:GFP set at:11-Jul-2019 19:42:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 19:42:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:42:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:42:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.925

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:00

Channel:Brightfield set at:11-Jul-2019 19:43:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 19:43:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:00

Channel configuration set to: GFP: 11-Jul-2019 19:43:01

Channel:GFP set at:11-Jul-2019 19:43:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 19:43:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:05

Channel:Brightfield set at:11-Jul-2019 19:43:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 19:43:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:05

Channel configuration set to: GFP: 11-Jul-2019 19:43:06

Channel:GFP set at:11-Jul-2019 19:43:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 19:43:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:10

Channel:Brightfield set at:11-Jul-2019 19:43:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 19:43:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:11

Channel configuration set to: GFP: 11-Jul-2019 19:43:12

Channel:GFP set at:11-Jul-2019 19:43:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 11-Jul-2019 19:43:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:16

Channel:Brightfield set at:11-Jul-2019 19:43:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 19:43:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:16

Channel configuration set to: GFP: 11-Jul-2019 19:43:17

Channel:GFP set at:11-Jul-2019 19:43:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 19:43:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:20

Channel:Brightfield set at:11-Jul-2019 19:43:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 19:43:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:21

Channel configuration set to: GFP: 11-Jul-2019 19:43:22

Channel:GFP set at:11-Jul-2019 19:43:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 11-Jul-2019 19:43:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:25

Channel:Brightfield set at:11-Jul-2019 19:43:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 19:43:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:26

Channel configuration set to: GFP: 11-Jul-2019 19:43:27

Channel:GFP set at:11-Jul-2019 19:43:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 19:43:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:31

Channel:Brightfield set at:11-Jul-2019 19:43:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 19:43:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:31

Channel configuration set to: GFP: 11-Jul-2019 19:43:32

Channel:GFP set at:11-Jul-2019 19:43:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 19:43:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:36

Channel:Brightfield set at:11-Jul-2019 19:43:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 19:43:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:36

Channel configuration set to: GFP: 11-Jul-2019 19:43:37

Channel:GFP set at:11-Jul-2019 19:43:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 19:43:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:41

Channel:Brightfield set at:11-Jul-2019 19:43:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 19:43:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:41

Channel configuration set to: GFP: 11-Jul-2019 19:43:42

Channel:GFP set at:11-Jul-2019 19:43:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 19:43:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:45

Channel:Brightfield set at:11-Jul-2019 19:43:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 19:43:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:46

Channel configuration set to: GFP: 11-Jul-2019 19:43:47

Channel:GFP set at:11-Jul-2019 19:43:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7751. 11-Jul-2019 19:43:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.025

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:50

Channel:Brightfield set at:11-Jul-2019 19:43:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 11-Jul-2019 19:43:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:51

Channel configuration set to: GFP: 11-Jul-2019 19:43:52

Channel:GFP set at:11-Jul-2019 19:43:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 19:43:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:54

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.675

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:55

Channel:Brightfield set at:11-Jul-2019 19:43:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0251. 11-Jul-2019 19:43:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:56

Channel configuration set to: GFP: 11-Jul-2019 19:43:57

Channel:GFP set at:11-Jul-2019 19:43:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 19:43:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:43:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:43:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 19:44:00

Channel:Brightfield set at:11-Jul-2019 19:44:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 19:44:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:44:01

Channel configuration set to: GFP: 11-Jul-2019 19:44:02

Channel:GFP set at:11-Jul-2019 19:44:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 19:44:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:44:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:40 completed at:11-Jul-2019 19:44:04

Time since start of timelapse:11790.8874

PFS is locked

Time to next time point:209.1883



------Time point_41------

Memory available for all arrays: 61075152896

Memory used by Matlab: 2206949376

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:35

Channel:Brightfield set at:11-Jul-2019 19:47:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 19:47:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:47:35

Channel configuration set to: GFP: 11-Jul-2019 19:47:37

Channel:GFP set at:11-Jul-2019 19:47:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 19:47:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:47:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.675

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:40

Channel:Brightfield set at:11-Jul-2019 19:47:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 19:47:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:47:40

Channel configuration set to: GFP: 11-Jul-2019 19:47:41

Channel:GFP set at:11-Jul-2019 19:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 11-Jul-2019 19:47:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:47:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.475

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:44

Channel:Brightfield set at:11-Jul-2019 19:47:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4251. 11-Jul-2019 19:47:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:47:45

Channel configuration set to: GFP: 11-Jul-2019 19:47:46

Channel:GFP set at:11-Jul-2019 19:47:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4251. 11-Jul-2019 19:47:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:47:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:49

Channel:Brightfield set at:11-Jul-2019 19:47:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 19:47:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:47:50

Channel configuration set to: GFP: 11-Jul-2019 19:47:51

Channel:GFP set at:11-Jul-2019 19:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 19:47:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:47:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.875

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:54

Channel:Brightfield set at:11-Jul-2019 19:47:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 19:47:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:47:55

Channel configuration set to: GFP: 11-Jul-2019 19:47:56

Channel:GFP set at:11-Jul-2019 19:47:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 19:47:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:47:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:47:59

Channel:Brightfield set at:11-Jul-2019 19:47:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 11-Jul-2019 19:47:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:47:59

Channel configuration set to: GFP: 11-Jul-2019 19:48:01

Channel:GFP set at:11-Jul-2019 19:48:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 11-Jul-2019 19:48:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:05

Channel:Brightfield set at:11-Jul-2019 19:48:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 19:48:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:05

Channel configuration set to: GFP: 11-Jul-2019 19:48:06

Channel:GFP set at:11-Jul-2019 19:48:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 19:48:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:10

Channel:Brightfield set at:11-Jul-2019 19:48:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 19:48:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:10

Channel configuration set to: GFP: 11-Jul-2019 19:48:12

Channel:GFP set at:11-Jul-2019 19:48:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 19:48:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.5

PFS not locked. Waiting for lock...

PFS took 0.700000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:15

Channel:Brightfield set at:11-Jul-2019 19:48:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 19:48:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:16

Channel configuration set to: GFP: 11-Jul-2019 19:48:17

Channel:GFP set at:11-Jul-2019 19:48:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 19:48:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:20

Channel:Brightfield set at:11-Jul-2019 19:48:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 19:48:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:21

Channel configuration set to: GFP: 11-Jul-2019 19:48:22

Channel:GFP set at:11-Jul-2019 19:48:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 19:48:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:25

Channel:Brightfield set at:11-Jul-2019 19:48:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 19:48:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:25

Channel configuration set to: GFP: 11-Jul-2019 19:48:27

Channel:GFP set at:11-Jul-2019 19:48:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 19:48:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:30

Channel:Brightfield set at:11-Jul-2019 19:48:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 11-Jul-2019 19:48:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:30

Channel configuration set to: GFP: 11-Jul-2019 19:48:31

Channel:GFP set at:11-Jul-2019 19:48:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 19:48:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:35

Channel:Brightfield set at:11-Jul-2019 19:48:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 19:48:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:36

Channel configuration set to: GFP: 11-Jul-2019 19:48:37

Channel:GFP set at:11-Jul-2019 19:48:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 11-Jul-2019 19:48:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:40

Channel:Brightfield set at:11-Jul-2019 19:48:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 19:48:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:40

Channel configuration set to: GFP: 11-Jul-2019 19:48:42

Channel:GFP set at:11-Jul-2019 19:48:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 19:48:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:44

Channel:Brightfield set at:11-Jul-2019 19:48:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 19:48:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:45

Channel configuration set to: GFP: 11-Jul-2019 19:48:46

Channel:GFP set at:11-Jul-2019 19:48:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 19:48:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.1

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:50

Channel:Brightfield set at:11-Jul-2019 19:48:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 11-Jul-2019 19:48:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:50

Channel configuration set to: GFP: 11-Jul-2019 19:48:51

Channel:GFP set at:11-Jul-2019 19:48:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 19:48:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:54

Channel:Brightfield set at:11-Jul-2019 19:48:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 19:48:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:54

Channel configuration set to: GFP: 11-Jul-2019 19:48:56

Channel:GFP set at:11-Jul-2019 19:48:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5501. 11-Jul-2019 19:48:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:48:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 19:48:59

Channel:Brightfield set at:11-Jul-2019 19:48:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 11-Jul-2019 19:49:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:49:00

Channel configuration set to: GFP: 11-Jul-2019 19:49:01

Channel:GFP set at:11-Jul-2019 19:49:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 11-Jul-2019 19:49:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:49:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:41 completed at:11-Jul-2019 19:49:03

Time since start of timelapse:12089.9295

PFS is locked

Time to next time point:210.1475



------Time point_42------

Memory available for all arrays: 61092085760

Memory used by Matlab: 2206949376

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:35

Channel:Brightfield set at:11-Jul-2019 19:52:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 19:52:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:52:35

Channel configuration set to: GFP: 11-Jul-2019 19:52:36

Channel:GFP set at:11-Jul-2019 19:52:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 19:52:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:52:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.6

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:40

Channel:Brightfield set at:11-Jul-2019 19:52:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 19:52:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:52:40

Channel configuration set to: GFP: 11-Jul-2019 19:52:41

Channel:GFP set at:11-Jul-2019 19:52:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 19:52:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:52:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:44

Channel:Brightfield set at:11-Jul-2019 19:52:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4751. 11-Jul-2019 19:52:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:52:45

Channel configuration set to: GFP: 11-Jul-2019 19:52:46

Channel:GFP set at:11-Jul-2019 19:52:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5251. 11-Jul-2019 19:52:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:52:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:49

Channel:Brightfield set at:11-Jul-2019 19:52:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 19:52:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:52:50

Channel configuration set to: GFP: 11-Jul-2019 19:52:51

Channel:GFP set at:11-Jul-2019 19:52:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 19:52:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:52:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:54

Channel:Brightfield set at:11-Jul-2019 19:52:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 19:52:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:52:55

Channel configuration set to: GFP: 11-Jul-2019 19:52:56

Channel:GFP set at:11-Jul-2019 19:52:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 19:52:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:52:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.9

Channel configuration set to: Brightfield: 11-Jul-2019 19:52:59

Channel:Brightfield set at:11-Jul-2019 19:52:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 11-Jul-2019 19:52:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:52:59

Channel configuration set to: GFP: 11-Jul-2019 19:53:01

Channel:GFP set at:11-Jul-2019 19:53:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 19:53:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:04

Channel:Brightfield set at:11-Jul-2019 19:53:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 19:53:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:04

Channel configuration set to: GFP: 11-Jul-2019 19:53:06

Channel:GFP set at:11-Jul-2019 19:53:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 19:53:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:09

Channel:Brightfield set at:11-Jul-2019 19:53:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 19:53:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:09

Channel configuration set to: GFP: 11-Jul-2019 19:53:11

Channel:GFP set at:11-Jul-2019 19:53:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 19:53:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:14

Channel:Brightfield set at:11-Jul-2019 19:53:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 19:53:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:15

Channel configuration set to: GFP: 11-Jul-2019 19:53:16

Channel:GFP set at:11-Jul-2019 19:53:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 19:53:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:19

Channel:Brightfield set at:11-Jul-2019 19:53:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 19:53:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:20

Channel configuration set to: GFP: 11-Jul-2019 19:53:21

Channel:GFP set at:11-Jul-2019 19:53:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 19:53:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:24

Channel:Brightfield set at:11-Jul-2019 19:53:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 19:53:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:25

Channel configuration set to: GFP: 11-Jul-2019 19:53:26

Channel:GFP set at:11-Jul-2019 19:53:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 19:53:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:29

Channel:Brightfield set at:11-Jul-2019 19:53:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 11-Jul-2019 19:53:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:30

Channel configuration set to: GFP: 11-Jul-2019 19:53:31

Channel:GFP set at:11-Jul-2019 19:53:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 11-Jul-2019 19:53:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:35

Channel:Brightfield set at:11-Jul-2019 19:53:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 19:53:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:35

Channel configuration set to: GFP: 11-Jul-2019 19:53:36

Channel:GFP set at:11-Jul-2019 19:53:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 19:53:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:39

Channel:Brightfield set at:11-Jul-2019 19:53:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 19:53:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:40

Channel configuration set to: GFP: 11-Jul-2019 19:53:41

Channel:GFP set at:11-Jul-2019 19:53:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 19:53:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:44

Channel:Brightfield set at:11-Jul-2019 19:53:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 19:53:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:45

Channel configuration set to: GFP: 11-Jul-2019 19:53:46

Channel:GFP set at:11-Jul-2019 19:53:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 19:53:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.95

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:49

Channel:Brightfield set at:11-Jul-2019 19:53:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 19:53:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:50

Channel configuration set to: GFP: 11-Jul-2019 19:53:51

Channel:GFP set at:11-Jul-2019 19:53:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 19:53:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.625

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:54

Channel:Brightfield set at:11-Jul-2019 19:53:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 19:53:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:55

Channel configuration set to: GFP: 11-Jul-2019 19:53:56

Channel:GFP set at:11-Jul-2019 19:53:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1001. 11-Jul-2019 19:53:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:53:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 11-Jul-2019 19:53:59

Channel:Brightfield set at:11-Jul-2019 19:53:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 19:54:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:54:00

Channel configuration set to: GFP: 11-Jul-2019 19:54:01

Channel:GFP set at:11-Jul-2019 19:54:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 19:54:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:54:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:42 completed at:11-Jul-2019 19:54:03

Time since start of timelapse:12389.8249

PFS is locked

Time to next time point:210.2534



------Time point_43------

Memory available for all arrays: 61111697408

Memory used by Matlab: 2207080448

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:35

Channel:Brightfield set at:11-Jul-2019 19:57:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 19:57:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:57:35

Channel configuration set to: GFP: 11-Jul-2019 19:57:36

Channel:GFP set at:11-Jul-2019 19:57:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 19:57:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:57:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.475

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:40

Channel:Brightfield set at:11-Jul-2019 19:57:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 19:57:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:57:40

Channel configuration set to: GFP: 11-Jul-2019 19:57:41

Channel:GFP set at:11-Jul-2019 19:57:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 11-Jul-2019 19:57:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:57:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.95

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:44

Channel:Brightfield set at:11-Jul-2019 19:57:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 11-Jul-2019 19:57:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:57:45

Channel configuration set to: GFP: 11-Jul-2019 19:57:46

Channel:GFP set at:11-Jul-2019 19:57:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 19:57:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:57:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:49

Channel:Brightfield set at:11-Jul-2019 19:57:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 19:57:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:57:50

Channel configuration set to: GFP: 11-Jul-2019 19:57:51

Channel:GFP set at:11-Jul-2019 19:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 19:57:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:57:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:54

Channel:Brightfield set at:11-Jul-2019 19:57:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 11-Jul-2019 19:57:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:57:54

Channel configuration set to: GFP: 11-Jul-2019 19:57:56

Channel:GFP set at:11-Jul-2019 19:57:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 11-Jul-2019 19:57:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:57:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.65

Channel configuration set to: Brightfield: 11-Jul-2019 19:57:59

Channel:Brightfield set at:11-Jul-2019 19:57:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 11-Jul-2019 19:57:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:57:59

Channel configuration set to: GFP: 11-Jul-2019 19:58:00

Channel:GFP set at:11-Jul-2019 19:58:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 11-Jul-2019 19:58:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:04

Channel:Brightfield set at:11-Jul-2019 19:58:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 19:58:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:05

Channel configuration set to: GFP: 11-Jul-2019 19:58:06

Channel:GFP set at:11-Jul-2019 19:58:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 19:58:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:10

Channel:Brightfield set at:11-Jul-2019 19:58:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 19:58:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:10

Channel configuration set to: GFP: 11-Jul-2019 19:58:11

Channel:GFP set at:11-Jul-2019 19:58:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 19:58:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:15

Channel:Brightfield set at:11-Jul-2019 19:58:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 11-Jul-2019 19:58:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:15

Channel configuration set to: GFP: 11-Jul-2019 19:58:17

Channel:GFP set at:11-Jul-2019 19:58:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 11-Jul-2019 19:58:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:20

Channel:Brightfield set at:11-Jul-2019 19:58:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 19:58:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:21

Channel configuration set to: GFP: 11-Jul-2019 19:58:22

Channel:GFP set at:11-Jul-2019 19:58:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 19:58:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:25

Channel:Brightfield set at:11-Jul-2019 19:58:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 11-Jul-2019 19:58:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:25

Channel configuration set to: GFP: 11-Jul-2019 19:58:27

Channel:GFP set at:11-Jul-2019 19:58:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 19:58:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:30

Channel:Brightfield set at:11-Jul-2019 19:58:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 11-Jul-2019 19:58:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:31

Channel configuration set to: GFP: 11-Jul-2019 19:58:32

Channel:GFP set at:11-Jul-2019 19:58:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 11-Jul-2019 19:58:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-4.675

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:35

Channel:Brightfield set at:11-Jul-2019 19:58:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 19:58:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:35

Channel configuration set to: GFP: 11-Jul-2019 19:58:37

Channel:GFP set at:11-Jul-2019 19:58:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 19:58:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:40

Channel:Brightfield set at:11-Jul-2019 19:58:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 19:58:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:40

Channel configuration set to: GFP: 11-Jul-2019 19:58:42

Channel:GFP set at:11-Jul-2019 19:58:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 19:58:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.6

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:45

Channel:Brightfield set at:11-Jul-2019 19:58:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 19:58:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:45

Channel configuration set to: GFP: 11-Jul-2019 19:58:47

Channel:GFP set at:11-Jul-2019 19:58:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 19:58:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.9

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:50

Channel:Brightfield set at:11-Jul-2019 19:58:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 19:58:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:50

Channel configuration set to: GFP: 11-Jul-2019 19:58:51

Channel:GFP set at:11-Jul-2019 19:58:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 19:58:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:54

Channel:Brightfield set at:11-Jul-2019 19:58:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 19:58:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:54

Channel configuration set to: GFP: 11-Jul-2019 19:58:56

Channel:GFP set at:11-Jul-2019 19:58:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 19:58:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:58:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 19:58:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 11-Jul-2019 19:59:00

Channel:Brightfield set at:11-Jul-2019 19:59:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 19:59:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:59:00

Channel configuration set to: GFP: 11-Jul-2019 19:59:01

Channel:GFP set at:11-Jul-2019 19:59:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 19:59:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 19:59:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:43 completed at:11-Jul-2019 19:59:03

Time since start of timelapse:12690.1171

PFS is locked

Time to next time point:209.9628



------Time point_44------

Memory available for all arrays: 61092651008

Memory used by Matlab: 2207211520

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:02:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.95

Channel configuration set to: Brightfield: 11-Jul-2019 20:02:35

Channel:Brightfield set at:11-Jul-2019 20:02:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 11-Jul-2019 20:02:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:02:35

Channel configuration set to: GFP: 11-Jul-2019 20:02:36

Channel:GFP set at:11-Jul-2019 20:02:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 11-Jul-2019 20:02:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:02:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:02:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.425

Channel configuration set to: Brightfield: 11-Jul-2019 20:02:40

Channel:Brightfield set at:11-Jul-2019 20:02:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 20:02:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:02:40

Channel configuration set to: GFP: 11-Jul-2019 20:02:41

Channel:GFP set at:11-Jul-2019 20:02:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 20:02:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:02:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:02:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 11-Jul-2019 20:02:44

Channel:Brightfield set at:11-Jul-2019 20:02:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7001. 11-Jul-2019 20:02:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:02:45

Channel configuration set to: GFP: 11-Jul-2019 20:02:46

Channel:GFP set at:11-Jul-2019 20:02:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.7001. 11-Jul-2019 20:02:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:02:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:02:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 11-Jul-2019 20:02:50

Channel:Brightfield set at:11-Jul-2019 20:02:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 20:02:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:02:50

Channel configuration set to: GFP: 11-Jul-2019 20:02:51

Channel:GFP set at:11-Jul-2019 20:02:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 20:02:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:02:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:02:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.525

Channel configuration set to: Brightfield: 11-Jul-2019 20:02:55

Channel:Brightfield set at:11-Jul-2019 20:02:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 11-Jul-2019 20:02:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:02:55

Channel configuration set to: GFP: 11-Jul-2019 20:02:56

Channel:GFP set at:11-Jul-2019 20:02:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 20:02:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:02:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:02:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.825

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:00

Channel:Brightfield set at:11-Jul-2019 20:03:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3001. 11-Jul-2019 20:03:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:00

Channel configuration set to: GFP: 11-Jul-2019 20:03:02

Channel:GFP set at:11-Jul-2019 20:03:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3001. 11-Jul-2019 20:03:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:05

Channel:Brightfield set at:11-Jul-2019 20:03:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 20:03:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:05

Channel configuration set to: GFP: 11-Jul-2019 20:03:06

Channel:GFP set at:11-Jul-2019 20:03:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 20:03:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:10

Channel:Brightfield set at:11-Jul-2019 20:03:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 20:03:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:10

Channel configuration set to: GFP: 11-Jul-2019 20:03:12

Channel:GFP set at:11-Jul-2019 20:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 20:03:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:15

Channel:Brightfield set at:11-Jul-2019 20:03:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 20:03:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:15

Channel configuration set to: GFP: 11-Jul-2019 20:03:16

Channel:GFP set at:11-Jul-2019 20:03:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5501. 11-Jul-2019 20:03:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:20

Channel:Brightfield set at:11-Jul-2019 20:03:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 20:03:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:20

Channel configuration set to: GFP: 11-Jul-2019 20:03:21

Channel:GFP set at:11-Jul-2019 20:03:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 20:03:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:25

Channel:Brightfield set at:11-Jul-2019 20:03:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 20:03:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:25

Channel configuration set to: GFP: 11-Jul-2019 20:03:27

Channel:GFP set at:11-Jul-2019 20:03:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1751. 11-Jul-2019 20:03:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:30

Channel:Brightfield set at:11-Jul-2019 20:03:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 11-Jul-2019 20:03:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:30

Channel configuration set to: GFP: 11-Jul-2019 20:03:31

Channel:GFP set at:11-Jul-2019 20:03:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 11-Jul-2019 20:03:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:35

Channel:Brightfield set at:11-Jul-2019 20:03:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1751. 11-Jul-2019 20:03:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:36

Channel configuration set to: GFP: 11-Jul-2019 20:03:37

Channel:GFP set at:11-Jul-2019 20:03:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 20:03:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.4

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:40

Channel:Brightfield set at:11-Jul-2019 20:03:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 20:03:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:41

Channel configuration set to: GFP: 11-Jul-2019 20:03:42

Channel:GFP set at:11-Jul-2019 20:03:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 11-Jul-2019 20:03:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:45

Channel:Brightfield set at:11-Jul-2019 20:03:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 11-Jul-2019 20:03:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:45

Channel configuration set to: GFP: 11-Jul-2019 20:03:47

Channel:GFP set at:11-Jul-2019 20:03:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 20:03:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.7

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:50

Channel:Brightfield set at:11-Jul-2019 20:03:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 20:03:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:50

Channel configuration set to: GFP: 11-Jul-2019 20:03:52

Channel:GFP set at:11-Jul-2019 20:03:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 11-Jul-2019 20:03:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:54

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.125

PFS not locked. Waiting for lock...

PFS took 0.400000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:55

Channel:Brightfield set at:11-Jul-2019 20:03:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4001. 11-Jul-2019 20:03:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:55

Channel configuration set to: GFP: 11-Jul-2019 20:03:56

Channel:GFP set at:11-Jul-2019 20:03:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4251. 11-Jul-2019 20:03:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:03:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:03:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 11-Jul-2019 20:04:00

Channel:Brightfield set at:11-Jul-2019 20:04:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 20:04:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:04:00

Channel configuration set to: GFP: 11-Jul-2019 20:04:01

Channel:GFP set at:11-Jul-2019 20:04:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 20:04:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:04:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:44 completed at:11-Jul-2019 20:04:04

Time since start of timelapse:12990.5152

PFS is locked

Time to next time point:209.5664



------Time point_45------

Memory available for all arrays: 61106085888

Memory used by Matlab: 2207272960

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:07:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.925

Channel configuration set to: Brightfield: 11-Jul-2019 20:07:35

Channel:Brightfield set at:11-Jul-2019 20:07:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 11-Jul-2019 20:07:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:07:35

Channel configuration set to: GFP: 11-Jul-2019 20:07:36

Channel:GFP set at:11-Jul-2019 20:07:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3251. 11-Jul-2019 20:07:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:07:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:07:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.475

Channel configuration set to: Brightfield: 11-Jul-2019 20:07:40

Channel:Brightfield set at:11-Jul-2019 20:07:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 20:07:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:07:40

Channel configuration set to: GFP: 11-Jul-2019 20:07:41

Channel:GFP set at:11-Jul-2019 20:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 20:07:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:07:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:07:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 11-Jul-2019 20:07:44

Channel:Brightfield set at:11-Jul-2019 20:07:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5751. 11-Jul-2019 20:07:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:07:45

Channel configuration set to: GFP: 11-Jul-2019 20:07:46

Channel:GFP set at:11-Jul-2019 20:07:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5751. 11-Jul-2019 20:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:07:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:07:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-7.55

PFS not locked. Waiting for lock...

PFS took 0.400000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:07:50

Channel:Brightfield set at:11-Jul-2019 20:07:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 20:07:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:07:50

Channel configuration set to: GFP: 11-Jul-2019 20:07:52

Channel:GFP set at:11-Jul-2019 20:07:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 20:07:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:07:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:07:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 11-Jul-2019 20:07:55

Channel:Brightfield set at:11-Jul-2019 20:07:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 20:07:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:07:56

Channel configuration set to: GFP: 11-Jul-2019 20:07:57

Channel:GFP set at:11-Jul-2019 20:07:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 20:07:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:07:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:07:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.225

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:00

Channel:Brightfield set at:11-Jul-2019 20:08:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 20:08:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:01

Channel configuration set to: GFP: 11-Jul-2019 20:08:02

Channel:GFP set at:11-Jul-2019 20:08:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 20:08:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:06

Channel:Brightfield set at:11-Jul-2019 20:08:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 20:08:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:06

Channel configuration set to: GFP: 11-Jul-2019 20:08:07

Channel:GFP set at:11-Jul-2019 20:08:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 20:08:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:10

Channel:Brightfield set at:11-Jul-2019 20:08:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 20:08:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:10

Channel configuration set to: GFP: 11-Jul-2019 20:08:12

Channel:GFP set at:11-Jul-2019 20:08:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 20:08:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:15

Channel:Brightfield set at:11-Jul-2019 20:08:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 11-Jul-2019 20:08:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:15

Channel configuration set to: GFP: 11-Jul-2019 20:08:16

Channel:GFP set at:11-Jul-2019 20:08:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 11-Jul-2019 20:08:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:20

Channel:Brightfield set at:11-Jul-2019 20:08:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6501. 11-Jul-2019 20:08:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:20

Channel configuration set to: GFP: 11-Jul-2019 20:08:21

Channel:GFP set at:11-Jul-2019 20:08:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 20:08:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:25

Channel:Brightfield set at:11-Jul-2019 20:08:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 20:08:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:25

Channel configuration set to: GFP: 11-Jul-2019 20:08:26

Channel:GFP set at:11-Jul-2019 20:08:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 20:08:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:30

Channel:Brightfield set at:11-Jul-2019 20:08:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 20:08:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:30

Channel configuration set to: GFP: 11-Jul-2019 20:08:32

Channel:GFP set at:11-Jul-2019 20:08:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 20:08:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:35

Channel:Brightfield set at:11-Jul-2019 20:08:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 20:08:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:36

Channel configuration set to: GFP: 11-Jul-2019 20:08:37

Channel:GFP set at:11-Jul-2019 20:08:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 20:08:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:40

Channel:Brightfield set at:11-Jul-2019 20:08:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 20:08:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:40

Channel configuration set to: GFP: 11-Jul-2019 20:08:42

Channel:GFP set at:11-Jul-2019 20:08:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 20:08:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:44

Channel:Brightfield set at:11-Jul-2019 20:08:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:08:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:45

Channel configuration set to: GFP: 11-Jul-2019 20:08:46

Channel:GFP set at:11-Jul-2019 20:08:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:08:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:49

Channel:Brightfield set at:11-Jul-2019 20:08:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 20:08:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:49

Channel configuration set to: GFP: 11-Jul-2019 20:08:51

Channel:GFP set at:11-Jul-2019 20:08:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 20:08:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:54

Channel:Brightfield set at:11-Jul-2019 20:08:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 11-Jul-2019 20:08:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:54

Channel configuration set to: GFP: 11-Jul-2019 20:08:55

Channel:GFP set at:11-Jul-2019 20:08:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 11-Jul-2019 20:08:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 11-Jul-2019 20:08:59

Channel:Brightfield set at:11-Jul-2019 20:08:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 20:08:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:08:59

Channel configuration set to: GFP: 11-Jul-2019 20:09:01

Channel:GFP set at:11-Jul-2019 20:09:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 20:09:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:09:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:45 completed at:11-Jul-2019 20:09:03

Time since start of timelapse:13289.7113

PFS is locked

Time to next time point:210.3717



------Time point_46------

Memory available for all arrays: 61100478464

Memory used by Matlab: 2207404032

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:35

Channel:Brightfield set at:11-Jul-2019 20:12:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 11-Jul-2019 20:12:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:12:35

Channel configuration set to: GFP: 11-Jul-2019 20:12:36

Channel:GFP set at:11-Jul-2019 20:12:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 20:12:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:12:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.6

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:39

Channel:Brightfield set at:11-Jul-2019 20:12:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 20:12:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:12:40

Channel configuration set to: GFP: 11-Jul-2019 20:12:41

Channel:GFP set at:11-Jul-2019 20:12:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 20:12:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:12:41

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.575

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:44

Channel:Brightfield set at:11-Jul-2019 20:12:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5751. 11-Jul-2019 20:12:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:12:44

Channel configuration set to: GFP: 11-Jul-2019 20:12:45

Channel:GFP set at:11-Jul-2019 20:12:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5751. 11-Jul-2019 20:12:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:12:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:49

Channel:Brightfield set at:11-Jul-2019 20:12:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 20:12:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:12:50

Channel configuration set to: GFP: 11-Jul-2019 20:12:51

Channel:GFP set at:11-Jul-2019 20:12:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 20:12:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:12:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:54

Channel:Brightfield set at:11-Jul-2019 20:12:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0501. 11-Jul-2019 20:12:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:12:54

Channel configuration set to: GFP: 11-Jul-2019 20:12:56

Channel:GFP set at:11-Jul-2019 20:12:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 20:12:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:12:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.725

Channel configuration set to: Brightfield: 11-Jul-2019 20:12:59

Channel:Brightfield set at:11-Jul-2019 20:12:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 11-Jul-2019 20:12:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:12:59

Channel configuration set to: GFP: 11-Jul-2019 20:13:00

Channel:GFP set at:11-Jul-2019 20:13:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 11-Jul-2019 20:13:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:04

Channel:Brightfield set at:11-Jul-2019 20:13:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 20:13:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:05

Channel configuration set to: GFP: 11-Jul-2019 20:13:06

Channel:GFP set at:11-Jul-2019 20:13:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 20:13:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:10

Channel:Brightfield set at:11-Jul-2019 20:13:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 20:13:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:10

Channel configuration set to: GFP: 11-Jul-2019 20:13:11

Channel:GFP set at:11-Jul-2019 20:13:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 20:13:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:14

Channel:Brightfield set at:11-Jul-2019 20:13:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 20:13:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:15

Channel configuration set to: GFP: 11-Jul-2019 20:13:16

Channel:GFP set at:11-Jul-2019 20:13:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 20:13:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:19

Channel:Brightfield set at:11-Jul-2019 20:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 20:13:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:20

Channel configuration set to: GFP: 11-Jul-2019 20:13:21

Channel:GFP set at:11-Jul-2019 20:13:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 20:13:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:24

Channel:Brightfield set at:11-Jul-2019 20:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 11-Jul-2019 20:13:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:24

Channel configuration set to: GFP: 11-Jul-2019 20:13:26

Channel:GFP set at:11-Jul-2019 20:13:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 11-Jul-2019 20:13:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.6

PFS not locked. Waiting for lock...

PFS took 0.150000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:28

Channel:Brightfield set at:11-Jul-2019 20:13:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 11-Jul-2019 20:13:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:29

Channel configuration set to: GFP: 11-Jul-2019 20:13:30

Channel:GFP set at:11-Jul-2019 20:13:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 11-Jul-2019 20:13:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:34

Channel:Brightfield set at:11-Jul-2019 20:13:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 20:13:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:35

Channel configuration set to: GFP: 11-Jul-2019 20:13:36

Channel:GFP set at:11-Jul-2019 20:13:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 20:13:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:39

Channel:Brightfield set at:11-Jul-2019 20:13:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 20:13:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:39

Channel configuration set to: GFP: 11-Jul-2019 20:13:41

Channel:GFP set at:11-Jul-2019 20:13:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 20:13:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.9

PFS not locked. Waiting for lock...

PFS took 0.250000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:44

Channel:Brightfield set at:11-Jul-2019 20:13:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 20:13:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:44

Channel configuration set to: GFP: 11-Jul-2019 20:13:45

Channel:GFP set at:11-Jul-2019 20:13:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 20:13:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.95

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:49

Channel:Brightfield set at:11-Jul-2019 20:13:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 20:13:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:49

Channel configuration set to: GFP: 11-Jul-2019 20:13:51

Channel:GFP set at:11-Jul-2019 20:13:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 20:13:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:52

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.5

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:54

Channel:Brightfield set at:11-Jul-2019 20:13:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2501. 11-Jul-2019 20:13:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:54

Channel configuration set to: GFP: 11-Jul-2019 20:13:55

Channel:GFP set at:11-Jul-2019 20:13:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2751. 11-Jul-2019 20:13:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 20:13:59

Channel:Brightfield set at:11-Jul-2019 20:13:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 20:13:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:13:59

Channel configuration set to: GFP: 11-Jul-2019 20:14:01

Channel:GFP set at:11-Jul-2019 20:14:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 20:14:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:14:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:46 completed at:11-Jul-2019 20:14:03

Time since start of timelapse:13589.7002

PFS is locked

Time to next time point:210.3845



------Time point_47------

Memory available for all arrays: 61108375552

Memory used by Matlab: 2207535104

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:17:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 11-Jul-2019 20:17:35

Channel:Brightfield set at:11-Jul-2019 20:17:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 11-Jul-2019 20:17:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:17:35

Channel configuration set to: GFP: 11-Jul-2019 20:17:36

Channel:GFP set at:11-Jul-2019 20:17:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 11-Jul-2019 20:17:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:17:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:17:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.65

Channel configuration set to: Brightfield: 11-Jul-2019 20:17:40

Channel:Brightfield set at:11-Jul-2019 20:17:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 20:17:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:17:40

Channel configuration set to: GFP: 11-Jul-2019 20:17:41

Channel:GFP set at:11-Jul-2019 20:17:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 20:17:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:17:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:17:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 11-Jul-2019 20:17:44

Channel:Brightfield set at:11-Jul-2019 20:17:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.4751. 11-Jul-2019 20:17:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:17:45

Channel configuration set to: GFP: 11-Jul-2019 20:17:46

Channel:GFP set at:11-Jul-2019 20:17:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.5001. 11-Jul-2019 20:17:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:17:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:17:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 11-Jul-2019 20:17:50

Channel:Brightfield set at:11-Jul-2019 20:17:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 20:17:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:17:50

Channel configuration set to: GFP: 11-Jul-2019 20:17:51

Channel:GFP set at:11-Jul-2019 20:17:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 20:17:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:17:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:17:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 11-Jul-2019 20:17:55

Channel:Brightfield set at:11-Jul-2019 20:17:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 20:17:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:17:55

Channel configuration set to: GFP: 11-Jul-2019 20:17:56

Channel:GFP set at:11-Jul-2019 20:17:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 20:17:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:17:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:17:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:00

Channel:Brightfield set at:11-Jul-2019 20:18:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 20:18:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:00

Channel configuration set to: GFP: 11-Jul-2019 20:18:01

Channel:GFP set at:11-Jul-2019 20:18:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 20:18:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:05

Channel:Brightfield set at:11-Jul-2019 20:18:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 20:18:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:05

Channel configuration set to: GFP: 11-Jul-2019 20:18:06

Channel:GFP set at:11-Jul-2019 20:18:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 20:18:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:10

Channel:Brightfield set at:11-Jul-2019 20:18:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 20:18:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:11

Channel configuration set to: GFP: 11-Jul-2019 20:18:12

Channel:GFP set at:11-Jul-2019 20:18:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 20:18:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:15

Channel:Brightfield set at:11-Jul-2019 20:18:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1751. 11-Jul-2019 20:18:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:15

Channel configuration set to: GFP: 11-Jul-2019 20:18:17

Channel:GFP set at:11-Jul-2019 20:18:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 20:18:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:20

Channel:Brightfield set at:11-Jul-2019 20:18:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 20:18:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:21

Channel configuration set to: GFP: 11-Jul-2019 20:18:22

Channel:GFP set at:11-Jul-2019 20:18:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5501. 11-Jul-2019 20:18:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:25

Channel:Brightfield set at:11-Jul-2019 20:18:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 11-Jul-2019 20:18:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:26

Channel configuration set to: GFP: 11-Jul-2019 20:18:27

Channel:GFP set at:11-Jul-2019 20:18:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 11-Jul-2019 20:18:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.6

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:31

Channel:Brightfield set at:11-Jul-2019 20:18:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 20:18:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:31

Channel configuration set to: GFP: 11-Jul-2019 20:18:32

Channel:GFP set at:11-Jul-2019 20:18:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 20:18:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:36

Channel:Brightfield set at:11-Jul-2019 20:18:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 20:18:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:36

Channel configuration set to: GFP: 11-Jul-2019 20:18:37

Channel:GFP set at:11-Jul-2019 20:18:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 20:18:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:41

Channel:Brightfield set at:11-Jul-2019 20:18:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 20:18:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:41

Channel configuration set to: GFP: 11-Jul-2019 20:18:42

Channel:GFP set at:11-Jul-2019 20:18:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 20:18:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:45

Channel:Brightfield set at:11-Jul-2019 20:18:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:18:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:46

Channel configuration set to: GFP: 11-Jul-2019 20:18:47

Channel:GFP set at:11-Jul-2019 20:18:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6751. 11-Jul-2019 20:18:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:48

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.9

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:50

Channel:Brightfield set at:11-Jul-2019 20:18:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 20:18:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:51

Channel configuration set to: GFP: 11-Jul-2019 20:18:52

Channel:GFP set at:11-Jul-2019 20:18:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5501. 11-Jul-2019 20:18:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:54

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.625

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:55

Channel:Brightfield set at:11-Jul-2019 20:18:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0751. 11-Jul-2019 20:18:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:56

Channel configuration set to: GFP: 11-Jul-2019 20:18:57

Channel:GFP set at:11-Jul-2019 20:18:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 20:18:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:18:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:18:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 20:19:00

Channel:Brightfield set at:11-Jul-2019 20:19:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 20:19:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:19:01

Channel configuration set to: GFP: 11-Jul-2019 20:19:02

Channel:GFP set at:11-Jul-2019 20:19:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 20:19:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:19:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:47 completed at:11-Jul-2019 20:19:04

Time since start of timelapse:13891.0022

PFS is locked

Time to next time point:209.084



------Time point_48------

Memory available for all arrays: 61106225152

Memory used by Matlab: 2207535104

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:22:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.225

Channel configuration set to: Brightfield: 11-Jul-2019 20:22:35

Channel:Brightfield set at:11-Jul-2019 20:22:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 20:22:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:22:35

Channel configuration set to: GFP: 11-Jul-2019 20:22:36

Channel:GFP set at:11-Jul-2019 20:22:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 20:22:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:22:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:22:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.75

Channel configuration set to: Brightfield: 11-Jul-2019 20:22:40

Channel:Brightfield set at:11-Jul-2019 20:22:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0751. 11-Jul-2019 20:22:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:22:40

Channel configuration set to: GFP: 11-Jul-2019 20:22:42

Channel:GFP set at:11-Jul-2019 20:22:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 20:22:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:22:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:22:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.075

Channel configuration set to: Brightfield: 11-Jul-2019 20:22:45

Channel:Brightfield set at:11-Jul-2019 20:22:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 20:22:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:22:46

Channel configuration set to: GFP: 11-Jul-2019 20:22:47

Channel:GFP set at:11-Jul-2019 20:22:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 20:22:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:22:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:22:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 11-Jul-2019 20:22:50

Channel:Brightfield set at:11-Jul-2019 20:22:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 20:22:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:22:50

Channel configuration set to: GFP: 11-Jul-2019 20:22:52

Channel:GFP set at:11-Jul-2019 20:22:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 11-Jul-2019 20:22:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:22:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:22:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.9

Channel configuration set to: Brightfield: 11-Jul-2019 20:22:55

Channel:Brightfield set at:11-Jul-2019 20:22:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1501. 11-Jul-2019 20:22:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:22:56

Channel configuration set to: GFP: 11-Jul-2019 20:22:57

Channel:GFP set at:11-Jul-2019 20:22:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1501. 11-Jul-2019 20:22:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:22:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:22:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:00

Channel:Brightfield set at:11-Jul-2019 20:23:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 20:23:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:00

Channel configuration set to: GFP: 11-Jul-2019 20:23:01

Channel:GFP set at:11-Jul-2019 20:23:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 20:23:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:06

Channel:Brightfield set at:11-Jul-2019 20:23:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 20:23:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:06

Channel configuration set to: GFP: 11-Jul-2019 20:23:08

Channel:GFP set at:11-Jul-2019 20:23:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 20:23:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:11

Channel:Brightfield set at:11-Jul-2019 20:23:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 20:23:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:12

Channel configuration set to: GFP: 11-Jul-2019 20:23:13

Channel:GFP set at:11-Jul-2019 20:23:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 20:23:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:16

Channel:Brightfield set at:11-Jul-2019 20:23:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 20:23:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:17

Channel configuration set to: GFP: 11-Jul-2019 20:23:18

Channel:GFP set at:11-Jul-2019 20:23:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 20:23:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:21

Channel:Brightfield set at:11-Jul-2019 20:23:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 11-Jul-2019 20:23:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:22

Channel configuration set to: GFP: 11-Jul-2019 20:23:23

Channel:GFP set at:11-Jul-2019 20:23:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 20:23:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:26

Channel:Brightfield set at:11-Jul-2019 20:23:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 20:23:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:26

Channel configuration set to: GFP: 11-Jul-2019 20:23:28

Channel:GFP set at:11-Jul-2019 20:23:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 20:23:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:31

Channel:Brightfield set at:11-Jul-2019 20:23:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 20:23:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:32

Channel configuration set to: GFP: 11-Jul-2019 20:23:33

Channel:GFP set at:11-Jul-2019 20:23:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 20:23:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:37

Channel:Brightfield set at:11-Jul-2019 20:23:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 20:23:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:37

Channel configuration set to: GFP: 11-Jul-2019 20:23:38

Channel:GFP set at:11-Jul-2019 20:23:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 20:23:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.7

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:41

Channel:Brightfield set at:11-Jul-2019 20:23:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 20:23:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:42

Channel configuration set to: GFP: 11-Jul-2019 20:23:43

Channel:GFP set at:11-Jul-2019 20:23:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 20:23:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:46

Channel:Brightfield set at:11-Jul-2019 20:23:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 20:23:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:46

Channel configuration set to: GFP: 11-Jul-2019 20:23:48

Channel:GFP set at:11-Jul-2019 20:23:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 11-Jul-2019 20:23:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:48

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:50

Channel:Brightfield set at:11-Jul-2019 20:23:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 20:23:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:51

Channel configuration set to: GFP: 11-Jul-2019 20:23:52

Channel:GFP set at:11-Jul-2019 20:23:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 20:23:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:53

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:54

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.4

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:56

Channel:Brightfield set at:11-Jul-2019 20:23:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 20:23:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:56

Channel configuration set to: GFP: 11-Jul-2019 20:23:57

Channel:GFP set at:11-Jul-2019 20:23:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 20:23:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:23:58

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:23:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 20:24:01

Channel:Brightfield set at:11-Jul-2019 20:24:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 20:24:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:24:01

Channel configuration set to: GFP: 11-Jul-2019 20:24:02

Channel:GFP set at:11-Jul-2019 20:24:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 20:24:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:24:03

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:48 completed at:11-Jul-2019 20:24:04

Time since start of timelapse:14191.4941

PFS is locked

Time to next time point:208.5938



------Time point_49------

Memory available for all arrays: 61105967104

Memory used by Matlab: 2207535104

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.4

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:35

Channel:Brightfield set at:11-Jul-2019 20:27:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 20:27:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:27:35

Channel configuration set to: GFP: 11-Jul-2019 20:27:36

Channel:GFP set at:11-Jul-2019 20:27:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 20:27:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:27:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.95

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:40

Channel:Brightfield set at:11-Jul-2019 20:27:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 20:27:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:27:40

Channel configuration set to: GFP: 11-Jul-2019 20:27:41

Channel:GFP set at:11-Jul-2019 20:27:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 20:27:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:27:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:45

Channel:Brightfield set at:11-Jul-2019 20:27:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 20:27:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:27:45

Channel configuration set to: GFP: 11-Jul-2019 20:27:46

Channel:GFP set at:11-Jul-2019 20:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 20:27:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:27:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.65

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:50

Channel:Brightfield set at:11-Jul-2019 20:27:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 11-Jul-2019 20:27:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:27:50

Channel configuration set to: GFP: 11-Jul-2019 20:27:51

Channel:GFP set at:11-Jul-2019 20:27:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 11-Jul-2019 20:27:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:27:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:55

Channel:Brightfield set at:11-Jul-2019 20:27:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 20:27:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:27:55

Channel configuration set to: GFP: 11-Jul-2019 20:27:56

Channel:GFP set at:11-Jul-2019 20:27:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 20:27:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:27:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 11-Jul-2019 20:27:59

Channel:Brightfield set at:11-Jul-2019 20:27:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 20:28:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:00

Channel configuration set to: GFP: 11-Jul-2019 20:28:01

Channel:GFP set at:11-Jul-2019 20:28:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 20:28:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:05

Channel:Brightfield set at:11-Jul-2019 20:28:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 20:28:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:05

Channel configuration set to: GFP: 11-Jul-2019 20:28:06

Channel:GFP set at:11-Jul-2019 20:28:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 20:28:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:10

Channel:Brightfield set at:11-Jul-2019 20:28:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 20:28:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:11

Channel configuration set to: GFP: 11-Jul-2019 20:28:12

Channel:GFP set at:11-Jul-2019 20:28:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 11-Jul-2019 20:28:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:16

Channel:Brightfield set at:11-Jul-2019 20:28:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 20:28:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:16

Channel configuration set to: GFP: 11-Jul-2019 20:28:18

Channel:GFP set at:11-Jul-2019 20:28:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 20:28:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:21

Channel:Brightfield set at:11-Jul-2019 20:28:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3001. 11-Jul-2019 20:28:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:22

Channel configuration set to: GFP: 11-Jul-2019 20:28:23

Channel:GFP set at:11-Jul-2019 20:28:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 11-Jul-2019 20:28:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:26

Channel:Brightfield set at:11-Jul-2019 20:28:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 11-Jul-2019 20:28:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:26

Channel configuration set to: GFP: 11-Jul-2019 20:28:28

Channel:GFP set at:11-Jul-2019 20:28:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 11-Jul-2019 20:28:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:31

Channel:Brightfield set at:11-Jul-2019 20:28:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 11-Jul-2019 20:28:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:31

Channel configuration set to: GFP: 11-Jul-2019 20:28:33

Channel:GFP set at:11-Jul-2019 20:28:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 11-Jul-2019 20:28:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:37

Channel:Brightfield set at:11-Jul-2019 20:28:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 20:28:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:37

Channel configuration set to: GFP: 11-Jul-2019 20:28:38

Channel:GFP set at:11-Jul-2019 20:28:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 20:28:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:41

Channel:Brightfield set at:11-Jul-2019 20:28:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 20:28:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:42

Channel configuration set to: GFP: 11-Jul-2019 20:28:43

Channel:GFP set at:11-Jul-2019 20:28:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 20:28:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:46

Channel:Brightfield set at:11-Jul-2019 20:28:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3001. 11-Jul-2019 20:28:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:46

Channel configuration set to: GFP: 11-Jul-2019 20:28:48

Channel:GFP set at:11-Jul-2019 20:28:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 20:28:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:48

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.175

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:51

Channel:Brightfield set at:11-Jul-2019 20:28:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 11-Jul-2019 20:28:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:51

Channel configuration set to: GFP: 11-Jul-2019 20:28:53

Channel:GFP set at:11-Jul-2019 20:28:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 20:28:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:53

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:55

Channel:Brightfield set at:11-Jul-2019 20:28:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 11-Jul-2019 20:28:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:56

Channel configuration set to: GFP: 11-Jul-2019 20:28:57

Channel:GFP set at:11-Jul-2019 20:28:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 20:28:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:28:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:28:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 11-Jul-2019 20:29:01

Channel:Brightfield set at:11-Jul-2019 20:29:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9501. 11-Jul-2019 20:29:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:29:01

Channel configuration set to: GFP: 11-Jul-2019 20:29:03

Channel:GFP set at:11-Jul-2019 20:29:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9501. 11-Jul-2019 20:29:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:29:03

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:49 completed at:11-Jul-2019 20:29:05

Time since start of timelapse:14491.5907

PFS is locked

Time to next time point:208.4985



------Time point_50------

Memory available for all arrays: 61107154944

Memory used by Matlab: 2207535104

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:32:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 11-Jul-2019 20:32:35

Channel:Brightfield set at:11-Jul-2019 20:32:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 20:32:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:32:35

Channel configuration set to: GFP: 11-Jul-2019 20:32:36

Channel:GFP set at:11-Jul-2019 20:32:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7751. 11-Jul-2019 20:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:32:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:32:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.05

Channel configuration set to: Brightfield: 11-Jul-2019 20:32:39

Channel:Brightfield set at:11-Jul-2019 20:32:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 11-Jul-2019 20:32:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:32:40

Channel configuration set to: GFP: 11-Jul-2019 20:32:41

Channel:GFP set at:11-Jul-2019 20:32:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 11-Jul-2019 20:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:32:41

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:32:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.375

Channel configuration set to: Brightfield: 11-Jul-2019 20:32:45

Channel:Brightfield set at:11-Jul-2019 20:32:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 20:32:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:32:45

Channel configuration set to: GFP: 11-Jul-2019 20:32:46

Channel:GFP set at:11-Jul-2019 20:32:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 20:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:32:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:32:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.725

Channel configuration set to: Brightfield: 11-Jul-2019 20:32:50

Channel:Brightfield set at:11-Jul-2019 20:32:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 20:32:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:32:50

Channel configuration set to: GFP: 11-Jul-2019 20:32:51

Channel:GFP set at:11-Jul-2019 20:32:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 20:32:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:32:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:32:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 11-Jul-2019 20:32:55

Channel:Brightfield set at:11-Jul-2019 20:32:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 20:32:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:32:55

Channel configuration set to: GFP: 11-Jul-2019 20:32:56

Channel:GFP set at:11-Jul-2019 20:32:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 20:32:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:32:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:32:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.9

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:00

Channel:Brightfield set at:11-Jul-2019 20:33:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 20:33:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:00

Channel configuration set to: GFP: 11-Jul-2019 20:33:02

Channel:GFP set at:11-Jul-2019 20:33:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 20:33:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:05

Channel:Brightfield set at:11-Jul-2019 20:33:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 20:33:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:05

Channel configuration set to: GFP: 11-Jul-2019 20:33:06

Channel:GFP set at:11-Jul-2019 20:33:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 20:33:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:10

Channel:Brightfield set at:11-Jul-2019 20:33:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 11-Jul-2019 20:33:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:11

Channel configuration set to: GFP: 11-Jul-2019 20:33:12

Channel:GFP set at:11-Jul-2019 20:33:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 20:33:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:15

Channel:Brightfield set at:11-Jul-2019 20:33:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 20:33:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:15

Channel configuration set to: GFP: 11-Jul-2019 20:33:17

Channel:GFP set at:11-Jul-2019 20:33:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 20:33:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:20

Channel:Brightfield set at:11-Jul-2019 20:33:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2251. 11-Jul-2019 20:33:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:21

Channel configuration set to: GFP: 11-Jul-2019 20:33:22

Channel:GFP set at:11-Jul-2019 20:33:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2251. 11-Jul-2019 20:33:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:25

Channel:Brightfield set at:11-Jul-2019 20:33:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 11-Jul-2019 20:33:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:25

Channel configuration set to: GFP: 11-Jul-2019 20:33:27

Channel:GFP set at:11-Jul-2019 20:33:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 11-Jul-2019 20:33:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:30

Channel:Brightfield set at:11-Jul-2019 20:33:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 20:33:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:31

Channel configuration set to: GFP: 11-Jul-2019 20:33:32

Channel:GFP set at:11-Jul-2019 20:33:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 20:33:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:36

Channel:Brightfield set at:11-Jul-2019 20:33:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 20:33:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:36

Channel configuration set to: GFP: 11-Jul-2019 20:33:37

Channel:GFP set at:11-Jul-2019 20:33:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 20:33:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:41

Channel:Brightfield set at:11-Jul-2019 20:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 20:33:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:41

Channel configuration set to: GFP: 11-Jul-2019 20:33:43

Channel:GFP set at:11-Jul-2019 20:33:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 20:33:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:46

Channel:Brightfield set at:11-Jul-2019 20:33:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 20:33:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:46

Channel configuration set to: GFP: 11-Jul-2019 20:33:47

Channel:GFP set at:11-Jul-2019 20:33:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 11-Jul-2019 20:33:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:48

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:51

Channel:Brightfield set at:11-Jul-2019 20:33:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 11-Jul-2019 20:33:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:52

Channel configuration set to: GFP: 11-Jul-2019 20:33:53

Channel:GFP set at:11-Jul-2019 20:33:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 11-Jul-2019 20:33:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:53

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 20:33:56

Channel:Brightfield set at:11-Jul-2019 20:33:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 20:33:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:56

Channel configuration set to: GFP: 11-Jul-2019 20:33:57

Channel:GFP set at:11-Jul-2019 20:33:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 20:33:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:33:58

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:34:00

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 11-Jul-2019 20:34:01

Channel:Brightfield set at:11-Jul-2019 20:34:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 20:34:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:34:02

Channel configuration set to: GFP: 11-Jul-2019 20:34:03

Channel:GFP set at:11-Jul-2019 20:34:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 20:34:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:34:03

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:50 completed at:11-Jul-2019 20:34:05

Time since start of timelapse:14792.1418

PFS is locked

Time to next time point:207.9492



------Time point_51------

Memory available for all arrays: 61106565120

Memory used by Matlab: 2207535104

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:37:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 11-Jul-2019 20:37:35

Channel:Brightfield set at:11-Jul-2019 20:37:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 20:37:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:37:35

Channel configuration set to: GFP: 11-Jul-2019 20:37:36

Channel:GFP set at:11-Jul-2019 20:37:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 20:37:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:37:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:37:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.05

Channel configuration set to: Brightfield: 11-Jul-2019 20:37:40

Channel:Brightfield set at:11-Jul-2019 20:37:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 20:37:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:37:40

Channel configuration set to: GFP: 11-Jul-2019 20:37:41

Channel:GFP set at:11-Jul-2019 20:37:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 11-Jul-2019 20:37:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:37:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:37:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 11-Jul-2019 20:37:45

Channel:Brightfield set at:11-Jul-2019 20:37:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 20:37:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:37:45

Channel configuration set to: GFP: 11-Jul-2019 20:37:47

Channel:GFP set at:11-Jul-2019 20:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 20:37:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:37:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:37:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 11-Jul-2019 20:37:49

Channel:Brightfield set at:11-Jul-2019 20:37:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 20:37:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:37:50

Channel configuration set to: GFP: 11-Jul-2019 20:37:51

Channel:GFP set at:11-Jul-2019 20:37:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 20:37:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:37:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:37:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 20:37:54

Channel:Brightfield set at:11-Jul-2019 20:37:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 20:37:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:37:55

Channel configuration set to: GFP: 11-Jul-2019 20:37:56

Channel:GFP set at:11-Jul-2019 20:37:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 20:37:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:37:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:37:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:00

Channel:Brightfield set at:11-Jul-2019 20:38:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 20:38:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:00

Channel configuration set to: GFP: 11-Jul-2019 20:38:01

Channel:GFP set at:11-Jul-2019 20:38:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 20:38:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:05

Channel:Brightfield set at:11-Jul-2019 20:38:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 20:38:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:05

Channel configuration set to: GFP: 11-Jul-2019 20:38:06

Channel:GFP set at:11-Jul-2019 20:38:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 20:38:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:09

Channel:Brightfield set at:11-Jul-2019 20:38:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 20:38:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:10

Channel configuration set to: GFP: 11-Jul-2019 20:38:11

Channel:GFP set at:11-Jul-2019 20:38:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 20:38:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:15

Channel:Brightfield set at:11-Jul-2019 20:38:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 20:38:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:15

Channel configuration set to: GFP: 11-Jul-2019 20:38:16

Channel:GFP set at:11-Jul-2019 20:38:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 20:38:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:19

Channel:Brightfield set at:11-Jul-2019 20:38:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 20:38:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:20

Channel configuration set to: GFP: 11-Jul-2019 20:38:21

Channel:GFP set at:11-Jul-2019 20:38:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 20:38:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:24

Channel:Brightfield set at:11-Jul-2019 20:38:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 11-Jul-2019 20:38:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:25

Channel configuration set to: GFP: 11-Jul-2019 20:38:26

Channel:GFP set at:11-Jul-2019 20:38:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 11-Jul-2019 20:38:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:29

Channel:Brightfield set at:11-Jul-2019 20:38:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 20:38:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:29

Channel configuration set to: GFP: 11-Jul-2019 20:38:31

Channel:GFP set at:11-Jul-2019 20:38:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 20:38:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:35

Channel:Brightfield set at:11-Jul-2019 20:38:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 20:38:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:35

Channel configuration set to: GFP: 11-Jul-2019 20:38:36

Channel:GFP set at:11-Jul-2019 20:38:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 20:38:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:39

Channel:Brightfield set at:11-Jul-2019 20:38:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 20:38:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:40

Channel configuration set to: GFP: 11-Jul-2019 20:38:41

Channel:GFP set at:11-Jul-2019 20:38:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 20:38:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:45

Channel:Brightfield set at:11-Jul-2019 20:38:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 20:38:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:45

Channel configuration set to: GFP: 11-Jul-2019 20:38:46

Channel:GFP set at:11-Jul-2019 20:38:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 20:38:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:49

Channel:Brightfield set at:11-Jul-2019 20:38:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 20:38:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:49

Channel configuration set to: GFP: 11-Jul-2019 20:38:51

Channel:GFP set at:11-Jul-2019 20:38:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 20:38:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.725

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:54

Channel:Brightfield set at:11-Jul-2019 20:38:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 20:38:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:55

Channel configuration set to: GFP: 11-Jul-2019 20:38:56

Channel:GFP set at:11-Jul-2019 20:38:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 11-Jul-2019 20:38:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:38:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 11-Jul-2019 20:38:59

Channel:Brightfield set at:11-Jul-2019 20:38:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 20:39:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:39:00

Channel configuration set to: GFP: 11-Jul-2019 20:39:01

Channel:GFP set at:11-Jul-2019 20:39:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 11-Jul-2019 20:39:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:39:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:51 completed at:11-Jul-2019 20:39:03

Time since start of timelapse:15090.1437

PFS is locked

Time to next time point:209.9489



------Time point_52------

Memory available for all arrays: 61105405952

Memory used by Matlab: 2207842304

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:42:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.575

Channel configuration set to: Brightfield: 11-Jul-2019 20:42:35

Channel:Brightfield set at:11-Jul-2019 20:42:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:42:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:42:35

Channel configuration set to: GFP: 11-Jul-2019 20:42:36

Channel:GFP set at:11-Jul-2019 20:42:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 11-Jul-2019 20:42:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:42:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:42:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.1

Channel configuration set to: Brightfield: 11-Jul-2019 20:42:40

Channel:Brightfield set at:11-Jul-2019 20:42:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7751. 11-Jul-2019 20:42:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:42:40

Channel configuration set to: GFP: 11-Jul-2019 20:42:42

Channel:GFP set at:11-Jul-2019 20:42:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 20:42:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:42:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:42:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.525

Channel configuration set to: Brightfield: 11-Jul-2019 20:42:45

Channel:Brightfield set at:11-Jul-2019 20:42:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 20:42:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:42:45

Channel configuration set to: GFP: 11-Jul-2019 20:42:47

Channel:GFP set at:11-Jul-2019 20:42:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 20:42:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:42:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:42:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.725

Channel configuration set to: Brightfield: 11-Jul-2019 20:42:50

Channel:Brightfield set at:11-Jul-2019 20:42:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 20:42:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:42:50

Channel configuration set to: GFP: 11-Jul-2019 20:42:52

Channel:GFP set at:11-Jul-2019 20:42:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 20:42:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:42:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:42:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 11-Jul-2019 20:42:55

Channel:Brightfield set at:11-Jul-2019 20:42:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 20:42:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:42:55

Channel configuration set to: GFP: 11-Jul-2019 20:42:57

Channel:GFP set at:11-Jul-2019 20:42:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 20:42:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:42:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:42:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:00

Channel:Brightfield set at:11-Jul-2019 20:43:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 20:43:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:01

Channel configuration set to: GFP: 11-Jul-2019 20:43:02

Channel:GFP set at:11-Jul-2019 20:43:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 20:43:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:05

Channel:Brightfield set at:11-Jul-2019 20:43:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 20:43:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:05

Channel configuration set to: GFP: 11-Jul-2019 20:43:07

Channel:GFP set at:11-Jul-2019 20:43:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 20:43:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:10

Channel:Brightfield set at:11-Jul-2019 20:43:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 20:43:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:11

Channel configuration set to: GFP: 11-Jul-2019 20:43:12

Channel:GFP set at:11-Jul-2019 20:43:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 20:43:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:15

Channel:Brightfield set at:11-Jul-2019 20:43:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 20:43:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:16

Channel configuration set to: GFP: 11-Jul-2019 20:43:17

Channel:GFP set at:11-Jul-2019 20:43:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 20:43:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:20

Channel:Brightfield set at:11-Jul-2019 20:43:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 11-Jul-2019 20:43:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:21

Channel configuration set to: GFP: 11-Jul-2019 20:43:22

Channel:GFP set at:11-Jul-2019 20:43:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 11-Jul-2019 20:43:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:25

Channel:Brightfield set at:11-Jul-2019 20:43:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 20:43:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:25

Channel configuration set to: GFP: 11-Jul-2019 20:43:27

Channel:GFP set at:11-Jul-2019 20:43:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 20:43:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:30

Channel:Brightfield set at:11-Jul-2019 20:43:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0001. 11-Jul-2019 20:43:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:30

Channel configuration set to: GFP: 11-Jul-2019 20:43:32

Channel:GFP set at:11-Jul-2019 20:43:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0001. 11-Jul-2019 20:43:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:32

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:36

Channel:Brightfield set at:11-Jul-2019 20:43:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 20:43:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:36

Channel configuration set to: GFP: 11-Jul-2019 20:43:37

Channel:GFP set at:11-Jul-2019 20:43:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 20:43:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:41

Channel:Brightfield set at:11-Jul-2019 20:43:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 20:43:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:41

Channel configuration set to: GFP: 11-Jul-2019 20:43:42

Channel:GFP set at:11-Jul-2019 20:43:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 20:43:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:45

Channel:Brightfield set at:11-Jul-2019 20:43:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 20:43:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:46

Channel configuration set to: GFP: 11-Jul-2019 20:43:47

Channel:GFP set at:11-Jul-2019 20:43:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 20:43:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.275

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:51

Channel:Brightfield set at:11-Jul-2019 20:43:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 20:43:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:51

Channel configuration set to: GFP: 11-Jul-2019 20:43:52

Channel:GFP set at:11-Jul-2019 20:43:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 20:43:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:53

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.7

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:55

Channel:Brightfield set at:11-Jul-2019 20:43:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 20:43:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:56

Channel configuration set to: GFP: 11-Jul-2019 20:43:57

Channel:GFP set at:11-Jul-2019 20:43:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 20:43:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:43:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:43:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 20:44:01

Channel:Brightfield set at:11-Jul-2019 20:44:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 20:44:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:44:01

Channel configuration set to: GFP: 11-Jul-2019 20:44:03

Channel:GFP set at:11-Jul-2019 20:44:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 11-Jul-2019 20:44:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:44:03

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:52 completed at:11-Jul-2019 20:44:05

Time since start of timelapse:15391.7336

PFS is locked

Time to next time point:208.3605



------Time point_53------

Memory available for all arrays: 61101006848

Memory used by Matlab: 2207916032

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:47:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.8

Channel configuration set to: Brightfield: 11-Jul-2019 20:47:35

Channel:Brightfield set at:11-Jul-2019 20:47:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 20:47:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:47:35

Channel configuration set to: GFP: 11-Jul-2019 20:47:36

Channel:GFP set at:11-Jul-2019 20:47:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 20:47:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:47:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:47:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.375

Channel configuration set to: Brightfield: 11-Jul-2019 20:47:39

Channel:Brightfield set at:11-Jul-2019 20:47:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:47:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:47:39

Channel configuration set to: GFP: 11-Jul-2019 20:47:41

Channel:GFP set at:11-Jul-2019 20:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:47:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:47:41

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:47:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.675

Channel configuration set to: Brightfield: 11-Jul-2019 20:47:44

Channel:Brightfield set at:11-Jul-2019 20:47:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 20:47:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:47:45

Channel configuration set to: GFP: 11-Jul-2019 20:47:46

Channel:GFP set at:11-Jul-2019 20:47:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 20:47:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:47:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:47:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.925

Channel configuration set to: Brightfield: 11-Jul-2019 20:47:49

Channel:Brightfield set at:11-Jul-2019 20:47:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 20:47:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:47:50

Channel configuration set to: GFP: 11-Jul-2019 20:47:51

Channel:GFP set at:11-Jul-2019 20:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 20:47:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:47:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:47:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 11-Jul-2019 20:47:55

Channel:Brightfield set at:11-Jul-2019 20:47:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 20:47:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:47:55

Channel configuration set to: GFP: 11-Jul-2019 20:47:56

Channel:GFP set at:11-Jul-2019 20:47:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 20:47:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:47:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:47:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:00

Channel:Brightfield set at:11-Jul-2019 20:48:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 20:48:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:00

Channel configuration set to: GFP: 11-Jul-2019 20:48:02

Channel:GFP set at:11-Jul-2019 20:48:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 20:48:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:05

Channel:Brightfield set at:11-Jul-2019 20:48:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 20:48:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:05

Channel configuration set to: GFP: 11-Jul-2019 20:48:07

Channel:GFP set at:11-Jul-2019 20:48:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 20:48:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:11

Channel:Brightfield set at:11-Jul-2019 20:48:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 20:48:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:11

Channel configuration set to: GFP: 11-Jul-2019 20:48:12

Channel:GFP set at:11-Jul-2019 20:48:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 20:48:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.825

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:16

Channel:Brightfield set at:11-Jul-2019 20:48:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 20:48:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:16

Channel configuration set to: GFP: 11-Jul-2019 20:48:18

Channel:GFP set at:11-Jul-2019 20:48:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 20:48:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:21

Channel:Brightfield set at:11-Jul-2019 20:48:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 20:48:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:21

Channel configuration set to: GFP: 11-Jul-2019 20:48:22

Channel:GFP set at:11-Jul-2019 20:48:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 20:48:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:26

Channel:Brightfield set at:11-Jul-2019 20:48:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 20:48:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:26

Channel configuration set to: GFP: 11-Jul-2019 20:48:27

Channel:GFP set at:11-Jul-2019 20:48:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 20:48:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:31

Channel:Brightfield set at:11-Jul-2019 20:48:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 11-Jul-2019 20:48:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:31

Channel configuration set to: GFP: 11-Jul-2019 20:48:33

Channel:GFP set at:11-Jul-2019 20:48:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 11-Jul-2019 20:48:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:36

Channel:Brightfield set at:11-Jul-2019 20:48:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 20:48:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:37

Channel configuration set to: GFP: 11-Jul-2019 20:48:38

Channel:GFP set at:11-Jul-2019 20:48:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 20:48:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:42

Channel:Brightfield set at:11-Jul-2019 20:48:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 20:48:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:42

Channel configuration set to: GFP: 11-Jul-2019 20:48:44

Channel:GFP set at:11-Jul-2019 20:48:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 20:48:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.575

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:47

Channel:Brightfield set at:11-Jul-2019 20:48:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 11-Jul-2019 20:48:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:47

Channel configuration set to: GFP: 11-Jul-2019 20:48:48

Channel:GFP set at:11-Jul-2019 20:48:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 20:48:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.525

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:52

Channel:Brightfield set at:11-Jul-2019 20:48:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8751. 11-Jul-2019 20:48:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:52

Channel configuration set to: GFP: 11-Jul-2019 20:48:53

Channel:GFP set at:11-Jul-2019 20:48:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8751. 11-Jul-2019 20:48:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 20:48:56

Channel:Brightfield set at:11-Jul-2019 20:48:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 11-Jul-2019 20:48:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:56

Channel configuration set to: GFP: 11-Jul-2019 20:48:58

Channel:GFP set at:11-Jul-2019 20:48:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 11-Jul-2019 20:48:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:48:58

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:49:00

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 20:49:01

Channel:Brightfield set at:11-Jul-2019 20:49:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:49:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:49:02

Channel configuration set to: GFP: 11-Jul-2019 20:49:03

Channel:GFP set at:11-Jul-2019 20:49:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:49:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:49:03

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:53 completed at:11-Jul-2019 20:49:05

Time since start of timelapse:15692.1818

PFS is locked

Time to next time point:207.9139



------Time point_54------

Memory available for all arrays: 61104697344

Memory used by Matlab: 2207916032

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.8

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:34

Channel:Brightfield set at:11-Jul-2019 20:52:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 20:52:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:52:34

Channel configuration set to: GFP: 11-Jul-2019 20:52:36

Channel:GFP set at:11-Jul-2019 20:52:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 20:52:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:52:36

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:39

Channel:Brightfield set at:11-Jul-2019 20:52:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 20:52:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:52:40

Channel configuration set to: GFP: 11-Jul-2019 20:52:41

Channel:GFP set at:11-Jul-2019 20:52:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 20:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:52:41

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.825

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:45

Channel:Brightfield set at:11-Jul-2019 20:52:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 20:52:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:52:45

Channel configuration set to: GFP: 11-Jul-2019 20:52:47

Channel:GFP set at:11-Jul-2019 20:52:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 20:52:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:52:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.125

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:50

Channel:Brightfield set at:11-Jul-2019 20:52:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 20:52:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:52:50

Channel configuration set to: GFP: 11-Jul-2019 20:52:52

Channel:GFP set at:11-Jul-2019 20:52:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 20:52:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:52:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:54

Channel:Brightfield set at:11-Jul-2019 20:52:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1751. 11-Jul-2019 20:52:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:52:55

Channel configuration set to: GFP: 11-Jul-2019 20:52:56

Channel:GFP set at:11-Jul-2019 20:52:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 20:52:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:52:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 11-Jul-2019 20:52:59

Channel:Brightfield set at:11-Jul-2019 20:52:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 20:52:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:52:59

Channel configuration set to: GFP: 11-Jul-2019 20:53:01

Channel:GFP set at:11-Jul-2019 20:53:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 20:53:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:05

Channel:Brightfield set at:11-Jul-2019 20:53:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 20:53:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:05

Channel configuration set to: GFP: 11-Jul-2019 20:53:06

Channel:GFP set at:11-Jul-2019 20:53:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 20:53:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:09

Channel:Brightfield set at:11-Jul-2019 20:53:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 11-Jul-2019 20:53:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:10

Channel configuration set to: GFP: 11-Jul-2019 20:53:11

Channel:GFP set at:11-Jul-2019 20:53:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 11-Jul-2019 20:53:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:14

Channel:Brightfield set at:11-Jul-2019 20:53:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 11-Jul-2019 20:53:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:14

Channel configuration set to: GFP: 11-Jul-2019 20:53:16

Channel:GFP set at:11-Jul-2019 20:53:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 11-Jul-2019 20:53:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:19

Channel:Brightfield set at:11-Jul-2019 20:53:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 20:53:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:20

Channel configuration set to: GFP: 11-Jul-2019 20:53:21

Channel:GFP set at:11-Jul-2019 20:53:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 20:53:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:24

Channel:Brightfield set at:11-Jul-2019 20:53:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 11-Jul-2019 20:53:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:24

Channel configuration set to: GFP: 11-Jul-2019 20:53:26

Channel:GFP set at:11-Jul-2019 20:53:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 11-Jul-2019 20:53:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:29

Channel:Brightfield set at:11-Jul-2019 20:53:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 11-Jul-2019 20:53:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:30

Channel configuration set to: GFP: 11-Jul-2019 20:53:31

Channel:GFP set at:11-Jul-2019 20:53:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 11-Jul-2019 20:53:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:35

Channel:Brightfield set at:11-Jul-2019 20:53:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 20:53:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:35

Channel configuration set to: GFP: 11-Jul-2019 20:53:36

Channel:GFP set at:11-Jul-2019 20:53:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 20:53:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:40

Channel:Brightfield set at:11-Jul-2019 20:53:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 20:53:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:40

Channel configuration set to: GFP: 11-Jul-2019 20:53:41

Channel:GFP set at:11-Jul-2019 20:53:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 20:53:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:44

Channel:Brightfield set at:11-Jul-2019 20:53:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 20:53:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:45

Channel configuration set to: GFP: 11-Jul-2019 20:53:46

Channel:GFP set at:11-Jul-2019 20:53:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 20:53:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:49

Channel:Brightfield set at:11-Jul-2019 20:53:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 20:53:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:49

Channel configuration set to: GFP: 11-Jul-2019 20:53:50

Channel:GFP set at:11-Jul-2019 20:53:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7751. 11-Jul-2019 20:53:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.025

PFS not locked. Waiting for lock...

PFS took 0.350000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:54

Channel:Brightfield set at:11-Jul-2019 20:53:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 20:53:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:54

Channel configuration set to: GFP: 11-Jul-2019 20:53:55

Channel:GFP set at:11-Jul-2019 20:53:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 20:53:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:57

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 20:53:59

Channel:Brightfield set at:11-Jul-2019 20:53:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:53:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:53:59

Channel configuration set to: GFP: 11-Jul-2019 20:54:01

Channel:GFP set at:11-Jul-2019 20:54:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:54:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:54:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:54 completed at:11-Jul-2019 20:54:03

Time since start of timelapse:15989.6678

PFS is locked

Time to next time point:210.4296



------Time point_55------

Memory available for all arrays: 61095944192

Memory used by Matlab: 2208108544

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:57:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.825

Channel configuration set to: Brightfield: 11-Jul-2019 20:57:35

Channel:Brightfield set at:11-Jul-2019 20:57:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 20:57:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:57:35

Channel configuration set to: GFP: 11-Jul-2019 20:57:36

Channel:GFP set at:11-Jul-2019 20:57:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 11-Jul-2019 20:57:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:57:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:57:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 11-Jul-2019 20:57:40

Channel:Brightfield set at:11-Jul-2019 20:57:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6751. 11-Jul-2019 20:57:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:57:40

Channel configuration set to: GFP: 11-Jul-2019 20:57:41

Channel:GFP set at:11-Jul-2019 20:57:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 20:57:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:57:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:57:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.65

Channel configuration set to: Brightfield: 11-Jul-2019 20:57:44

Channel:Brightfield set at:11-Jul-2019 20:57:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 11-Jul-2019 20:57:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:57:45

Channel configuration set to: GFP: 11-Jul-2019 20:57:46

Channel:GFP set at:11-Jul-2019 20:57:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 20:57:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:57:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:57:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.1

Channel configuration set to: Brightfield: 11-Jul-2019 20:57:50

Channel:Brightfield set at:11-Jul-2019 20:57:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 20:57:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:57:50

Channel configuration set to: GFP: 11-Jul-2019 20:57:51

Channel:GFP set at:11-Jul-2019 20:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 20:57:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:57:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:57:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.425

Channel configuration set to: Brightfield: 11-Jul-2019 20:57:55

Channel:Brightfield set at:11-Jul-2019 20:57:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 20:57:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:57:55

Channel configuration set to: GFP: 11-Jul-2019 20:57:56

Channel:GFP set at:11-Jul-2019 20:57:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 20:57:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:57:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 20:57:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.6

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:00

Channel:Brightfield set at:11-Jul-2019 20:58:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 20:58:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:00

Channel configuration set to: GFP: 11-Jul-2019 20:58:02

Channel:GFP set at:11-Jul-2019 20:58:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 20:58:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:05

Channel:Brightfield set at:11-Jul-2019 20:58:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 20:58:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:06

Channel configuration set to: GFP: 11-Jul-2019 20:58:07

Channel:GFP set at:11-Jul-2019 20:58:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 20:58:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:11

Channel:Brightfield set at:11-Jul-2019 20:58:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 20:58:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:11

Channel configuration set to: GFP: 11-Jul-2019 20:58:13

Channel:GFP set at:11-Jul-2019 20:58:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 20:58:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:16

Channel:Brightfield set at:11-Jul-2019 20:58:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 20:58:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:17

Channel configuration set to: GFP: 11-Jul-2019 20:58:18

Channel:GFP set at:11-Jul-2019 20:58:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 20:58:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:21

Channel:Brightfield set at:11-Jul-2019 20:58:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 11-Jul-2019 20:58:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:21

Channel configuration set to: GFP: 11-Jul-2019 20:58:23

Channel:GFP set at:11-Jul-2019 20:58:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0001. 11-Jul-2019 20:58:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:26

Channel:Brightfield set at:11-Jul-2019 20:58:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 11-Jul-2019 20:58:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:26

Channel configuration set to: GFP: 11-Jul-2019 20:58:28

Channel:GFP set at:11-Jul-2019 20:58:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 11-Jul-2019 20:58:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:31

Channel:Brightfield set at:11-Jul-2019 20:58:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 11-Jul-2019 20:58:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:32

Channel configuration set to: GFP: 11-Jul-2019 20:58:33

Channel:GFP set at:11-Jul-2019 20:58:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 11-Jul-2019 20:58:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.35

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:36

Channel:Brightfield set at:11-Jul-2019 20:58:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 11-Jul-2019 20:58:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:36

Channel configuration set to: GFP: 11-Jul-2019 20:58:38

Channel:GFP set at:11-Jul-2019 20:58:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 20:58:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:41

Channel:Brightfield set at:11-Jul-2019 20:58:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 20:58:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:41

Channel configuration set to: GFP: 11-Jul-2019 20:58:43

Channel:GFP set at:11-Jul-2019 20:58:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 20:58:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:45

Channel:Brightfield set at:11-Jul-2019 20:58:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 20:58:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:46

Channel configuration set to: GFP: 11-Jul-2019 20:58:47

Channel:GFP set at:11-Jul-2019 20:58:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 20:58:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:50

Channel:Brightfield set at:11-Jul-2019 20:58:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 20:58:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:50

Channel configuration set to: GFP: 11-Jul-2019 20:58:52

Channel:GFP set at:11-Jul-2019 20:58:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 20:58:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:54

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:55

Channel:Brightfield set at:11-Jul-2019 20:58:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 20:58:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:55

Channel configuration set to: GFP: 11-Jul-2019 20:58:56

Channel:GFP set at:11-Jul-2019 20:58:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 20:58:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:58:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 20:58:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 20:59:00

Channel:Brightfield set at:11-Jul-2019 20:59:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:59:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:59:00

Channel configuration set to: GFP: 11-Jul-2019 20:59:02

Channel:GFP set at:11-Jul-2019 20:59:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 20:59:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 20:59:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:55 completed at:11-Jul-2019 20:59:04

Time since start of timelapse:16290.8653

PFS is locked

Time to next time point:209.2338



------Time point_56------

Memory available for all arrays: 61092212736

Memory used by Matlab: 2208239616

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:02:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.825

Channel configuration set to: Brightfield: 11-Jul-2019 21:02:35

Channel:Brightfield set at:11-Jul-2019 21:02:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 11-Jul-2019 21:02:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:02:35

Channel configuration set to: GFP: 11-Jul-2019 21:02:36

Channel:GFP set at:11-Jul-2019 21:02:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 11-Jul-2019 21:02:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:02:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:02:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.275

Channel configuration set to: Brightfield: 11-Jul-2019 21:02:40

Channel:Brightfield set at:11-Jul-2019 21:02:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6251. 11-Jul-2019 21:02:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:02:40

Channel configuration set to: GFP: 11-Jul-2019 21:02:41

Channel:GFP set at:11-Jul-2019 21:02:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6251. 11-Jul-2019 21:02:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:02:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:02:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.7

Channel configuration set to: Brightfield: 11-Jul-2019 21:02:45

Channel:Brightfield set at:11-Jul-2019 21:02:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 21:02:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:02:45

Channel configuration set to: GFP: 11-Jul-2019 21:02:47

Channel:GFP set at:11-Jul-2019 21:02:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 21:02:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:02:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:02:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.125

Channel configuration set to: Brightfield: 11-Jul-2019 21:02:50

Channel:Brightfield set at:11-Jul-2019 21:02:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 21:02:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:02:50

Channel configuration set to: GFP: 11-Jul-2019 21:02:52

Channel:GFP set at:11-Jul-2019 21:02:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 21:02:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:02:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:02:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.475

Channel configuration set to: Brightfield: 11-Jul-2019 21:02:55

Channel:Brightfield set at:11-Jul-2019 21:02:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 21:02:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:02:56

Channel configuration set to: GFP: 11-Jul-2019 21:02:57

Channel:GFP set at:11-Jul-2019 21:02:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 11-Jul-2019 21:02:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:02:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:02:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.2

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:00

Channel:Brightfield set at:11-Jul-2019 21:03:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 21:03:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:01

Channel configuration set to: GFP: 11-Jul-2019 21:03:02

Channel:GFP set at:11-Jul-2019 21:03:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 21:03:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:05

Channel:Brightfield set at:11-Jul-2019 21:03:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 21:03:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:05

Channel configuration set to: GFP: 11-Jul-2019 21:03:07

Channel:GFP set at:11-Jul-2019 21:03:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 21:03:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:11

Channel:Brightfield set at:11-Jul-2019 21:03:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 21:03:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:11

Channel configuration set to: GFP: 11-Jul-2019 21:03:12

Channel:GFP set at:11-Jul-2019 21:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 21:03:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.775

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:16

Channel:Brightfield set at:11-Jul-2019 21:03:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 21:03:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:16

Channel configuration set to: GFP: 11-Jul-2019 21:03:18

Channel:GFP set at:11-Jul-2019 21:03:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 21:03:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:21

Channel:Brightfield set at:11-Jul-2019 21:03:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 21:03:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:21

Channel configuration set to: GFP: 11-Jul-2019 21:03:22

Channel:GFP set at:11-Jul-2019 21:03:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 21:03:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.8

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:25

Channel:Brightfield set at:11-Jul-2019 21:03:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 11-Jul-2019 21:03:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:26

Channel configuration set to: GFP: 11-Jul-2019 21:03:27

Channel:GFP set at:11-Jul-2019 21:03:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 21:03:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:31

Channel:Brightfield set at:11-Jul-2019 21:03:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 11-Jul-2019 21:03:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:31

Channel configuration set to: GFP: 11-Jul-2019 21:03:33

Channel:GFP set at:11-Jul-2019 21:03:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 11-Jul-2019 21:03:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:36

Channel:Brightfield set at:11-Jul-2019 21:03:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 21:03:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:37

Channel configuration set to: GFP: 11-Jul-2019 21:03:38

Channel:GFP set at:11-Jul-2019 21:03:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 11-Jul-2019 21:03:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:41

Channel:Brightfield set at:11-Jul-2019 21:03:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 21:03:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:41

Channel configuration set to: GFP: 11-Jul-2019 21:03:43

Channel:GFP set at:11-Jul-2019 21:03:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 11-Jul-2019 21:03:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:46

Channel:Brightfield set at:11-Jul-2019 21:03:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 21:03:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:46

Channel configuration set to: GFP: 11-Jul-2019 21:03:48

Channel:GFP set at:11-Jul-2019 21:03:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 21:03:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:48

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:51

Channel:Brightfield set at:11-Jul-2019 21:03:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 21:03:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:51

Channel configuration set to: GFP: 11-Jul-2019 21:03:53

Channel:GFP set at:11-Jul-2019 21:03:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 21:03:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:53

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 11-Jul-2019 21:03:56

Channel:Brightfield set at:11-Jul-2019 21:03:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 21:03:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:56

Channel configuration set to: GFP: 11-Jul-2019 21:03:58

Channel:GFP set at:11-Jul-2019 21:03:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 21:03:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:03:58

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:04:00

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 11-Jul-2019 21:04:01

Channel:Brightfield set at:11-Jul-2019 21:04:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 21:04:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:04:01

Channel configuration set to: GFP: 11-Jul-2019 21:04:03

Channel:GFP set at:11-Jul-2019 21:04:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 21:04:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:04:03

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:56 completed at:11-Jul-2019 21:04:05

Time since start of timelapse:16591.9075

PFS is locked

Time to next time point:208.1932



------Time point_57------

Memory available for all arrays: 61113020416

Memory used by Matlab: 2208833536

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:07:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.925

Channel configuration set to: Brightfield: 11-Jul-2019 21:07:35

Channel:Brightfield set at:11-Jul-2019 21:07:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 21:07:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:07:35

Channel configuration set to: GFP: 11-Jul-2019 21:07:36

Channel:GFP set at:11-Jul-2019 21:07:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 21:07:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:07:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:07:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 11-Jul-2019 21:07:40

Channel:Brightfield set at:11-Jul-2019 21:07:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 21:07:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:07:40

Channel configuration set to: GFP: 11-Jul-2019 21:07:41

Channel:GFP set at:11-Jul-2019 21:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 21:07:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:07:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:07:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.8

Channel configuration set to: Brightfield: 11-Jul-2019 21:07:45

Channel:Brightfield set at:11-Jul-2019 21:07:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 21:07:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:07:45

Channel configuration set to: GFP: 11-Jul-2019 21:07:47

Channel:GFP set at:11-Jul-2019 21:07:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1251. 11-Jul-2019 21:07:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:07:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:07:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9

Channel configuration set to: Brightfield: 11-Jul-2019 21:07:50

Channel:Brightfield set at:11-Jul-2019 21:07:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 21:07:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:07:50

Channel configuration set to: GFP: 11-Jul-2019 21:07:52

Channel:GFP set at:11-Jul-2019 21:07:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 21:07:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:07:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:07:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 11-Jul-2019 21:07:55

Channel:Brightfield set at:11-Jul-2019 21:07:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 21:07:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:07:56

Channel configuration set to: GFP: 11-Jul-2019 21:07:57

Channel:GFP set at:11-Jul-2019 21:07:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 21:07:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:07:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:07:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:00

Channel:Brightfield set at:11-Jul-2019 21:08:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 11-Jul-2019 21:08:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:00

Channel configuration set to: GFP: 11-Jul-2019 21:08:01

Channel:GFP set at:11-Jul-2019 21:08:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 11-Jul-2019 21:08:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:05

Channel:Brightfield set at:11-Jul-2019 21:08:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 11-Jul-2019 21:08:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:05

Channel configuration set to: GFP: 11-Jul-2019 21:08:06

Channel:GFP set at:11-Jul-2019 21:08:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 11-Jul-2019 21:08:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:10

Channel:Brightfield set at:11-Jul-2019 21:08:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 21:08:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:10

Channel configuration set to: GFP: 11-Jul-2019 21:08:11

Channel:GFP set at:11-Jul-2019 21:08:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 21:08:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:15

Channel:Brightfield set at:11-Jul-2019 21:08:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 21:08:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:15

Channel configuration set to: GFP: 11-Jul-2019 21:08:16

Channel:GFP set at:11-Jul-2019 21:08:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 21:08:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:20

Channel:Brightfield set at:11-Jul-2019 21:08:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 21:08:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:20

Channel configuration set to: GFP: 11-Jul-2019 21:08:21

Channel:GFP set at:11-Jul-2019 21:08:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9251. 11-Jul-2019 21:08:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:24

Channel:Brightfield set at:11-Jul-2019 21:08:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 21:08:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:25

Channel configuration set to: GFP: 11-Jul-2019 21:08:26

Channel:GFP set at:11-Jul-2019 21:08:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 21:08:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:29

Channel:Brightfield set at:11-Jul-2019 21:08:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 11-Jul-2019 21:08:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:29

Channel configuration set to: GFP: 11-Jul-2019 21:08:31

Channel:GFP set at:11-Jul-2019 21:08:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 11-Jul-2019 21:08:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:35

Channel:Brightfield set at:11-Jul-2019 21:08:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 21:08:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:35

Channel configuration set to: GFP: 11-Jul-2019 21:08:36

Channel:GFP set at:11-Jul-2019 21:08:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 21:08:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:39

Channel:Brightfield set at:11-Jul-2019 21:08:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 21:08:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:40

Channel configuration set to: GFP: 11-Jul-2019 21:08:41

Channel:GFP set at:11-Jul-2019 21:08:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 11-Jul-2019 21:08:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:45

Channel:Brightfield set at:11-Jul-2019 21:08:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 21:08:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:45

Channel configuration set to: GFP: 11-Jul-2019 21:08:47

Channel:GFP set at:11-Jul-2019 21:08:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 11-Jul-2019 21:08:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:50

Channel:Brightfield set at:11-Jul-2019 21:08:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 21:08:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:51

Channel configuration set to: GFP: 11-Jul-2019 21:08:52

Channel:GFP set at:11-Jul-2019 21:08:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7251. 11-Jul-2019 21:08:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:54

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:55

Channel:Brightfield set at:11-Jul-2019 21:08:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 21:08:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:56

Channel configuration set to: GFP: 11-Jul-2019 21:08:57

Channel:GFP set at:11-Jul-2019 21:08:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 21:08:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:08:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:08:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 21:09:00

Channel:Brightfield set at:11-Jul-2019 21:09:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 21:09:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:09:01

Channel configuration set to: GFP: 11-Jul-2019 21:09:02

Channel:GFP set at:11-Jul-2019 21:09:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 21:09:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:09:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:57 completed at:11-Jul-2019 21:09:04

Time since start of timelapse:16891.3944

PFS is locked

Time to next time point:208.7078



------Time point_58------

Memory available for all arrays: 61100244992

Memory used by Matlab: 2208833536

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.975

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:35

Channel:Brightfield set at:11-Jul-2019 21:12:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 21:12:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:12:35

Channel configuration set to: GFP: 11-Jul-2019 21:12:36

Channel:GFP set at:11-Jul-2019 21:12:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 21:12:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:12:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.4

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:39

Channel:Brightfield set at:11-Jul-2019 21:12:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5501. 11-Jul-2019 21:12:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:12:39

Channel configuration set to: GFP: 11-Jul-2019 21:12:41

Channel:GFP set at:11-Jul-2019 21:12:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 21:12:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:12:41

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:43

Channel:Brightfield set at:11-Jul-2019 21:12:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7501. 11-Jul-2019 21:12:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:12:44

Channel configuration set to: GFP: 11-Jul-2019 21:12:45

Channel:GFP set at:11-Jul-2019 21:12:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 21:12:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:12:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.125

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:49

Channel:Brightfield set at:11-Jul-2019 21:12:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 21:12:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:12:49

Channel configuration set to: GFP: 11-Jul-2019 21:12:51

Channel:GFP set at:11-Jul-2019 21:12:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 21:12:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:12:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.575

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:54

Channel:Brightfield set at:11-Jul-2019 21:12:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 21:12:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:12:55

Channel configuration set to: GFP: 11-Jul-2019 21:12:56

Channel:GFP set at:11-Jul-2019 21:12:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 21:12:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:12:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 11-Jul-2019 21:12:59

Channel:Brightfield set at:11-Jul-2019 21:12:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1251. 11-Jul-2019 21:12:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:12:59

Channel configuration set to: GFP: 11-Jul-2019 21:13:00

Channel:GFP set at:11-Jul-2019 21:13:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1251. 11-Jul-2019 21:13:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:04

Channel:Brightfield set at:11-Jul-2019 21:13:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 11-Jul-2019 21:13:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:05

Channel configuration set to: GFP: 11-Jul-2019 21:13:06

Channel:GFP set at:11-Jul-2019 21:13:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 11-Jul-2019 21:13:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:09

Channel:Brightfield set at:11-Jul-2019 21:13:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:13:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:10

Channel configuration set to: GFP: 11-Jul-2019 21:13:11

Channel:GFP set at:11-Jul-2019 21:13:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:13:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.85

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:14

Channel:Brightfield set at:11-Jul-2019 21:13:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 21:13:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:14

Channel configuration set to: GFP: 11-Jul-2019 21:13:16

Channel:GFP set at:11-Jul-2019 21:13:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 11-Jul-2019 21:13:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:19

Channel:Brightfield set at:11-Jul-2019 21:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 21:13:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:19

Channel configuration set to: GFP: 11-Jul-2019 21:13:21

Channel:GFP set at:11-Jul-2019 21:13:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 21:13:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:24

Channel:Brightfield set at:11-Jul-2019 21:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 11-Jul-2019 21:13:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:24

Channel configuration set to: GFP: 11-Jul-2019 21:13:26

Channel:GFP set at:11-Jul-2019 21:13:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 21:13:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:28

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:29

Channel:Brightfield set at:11-Jul-2019 21:13:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 21:13:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:30

Channel configuration set to: GFP: 11-Jul-2019 21:13:31

Channel:GFP set at:11-Jul-2019 21:13:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 21:13:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:35

Channel:Brightfield set at:11-Jul-2019 21:13:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 21:13:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:35

Channel configuration set to: GFP: 11-Jul-2019 21:13:36

Channel:GFP set at:11-Jul-2019 21:13:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 21:13:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:39

Channel:Brightfield set at:11-Jul-2019 21:13:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:13:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:40

Channel configuration set to: GFP: 11-Jul-2019 21:13:41

Channel:GFP set at:11-Jul-2019 21:13:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:13:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:41

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:44

Channel:Brightfield set at:11-Jul-2019 21:13:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 21:13:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:45

Channel configuration set to: GFP: 11-Jul-2019 21:13:46

Channel:GFP set at:11-Jul-2019 21:13:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 21:13:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:49

Channel:Brightfield set at:11-Jul-2019 21:13:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 21:13:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:49

Channel configuration set to: GFP: 11-Jul-2019 21:13:51

Channel:GFP set at:11-Jul-2019 21:13:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 21:13:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:51

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.35

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:54

Channel:Brightfield set at:11-Jul-2019 21:13:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 11-Jul-2019 21:13:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:55

Channel configuration set to: GFP: 11-Jul-2019 21:13:56

Channel:GFP set at:11-Jul-2019 21:13:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 11-Jul-2019 21:13:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:13:56

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:58

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 11-Jul-2019 21:13:59

Channel:Brightfield set at:11-Jul-2019 21:13:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 21:14:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:14:00

Channel configuration set to: GFP: 11-Jul-2019 21:14:01

Channel:GFP set at:11-Jul-2019 21:14:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 21:14:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:14:01

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:58 completed at:11-Jul-2019 21:14:03

Time since start of timelapse:17190.0995

PFS is locked

Time to next time point:210.004



------Time point_59------

Memory available for all arrays: 61103439872

Memory used by Matlab: 2208833536

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:17:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.05

Channel configuration set to: Brightfield: 11-Jul-2019 21:17:34

Channel:Brightfield set at:11-Jul-2019 21:17:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 21:17:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:17:35

Channel configuration set to: GFP: 11-Jul-2019 21:17:36

Channel:GFP set at:11-Jul-2019 21:17:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 21:17:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:17:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:17:38

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.4

Channel configuration set to: Brightfield: 11-Jul-2019 21:17:39

Channel:Brightfield set at:11-Jul-2019 21:17:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 21:17:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:17:40

Channel configuration set to: GFP: 11-Jul-2019 21:17:41

Channel:GFP set at:11-Jul-2019 21:17:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4751. 11-Jul-2019 21:17:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:17:41

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:17:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.875

Channel configuration set to: Brightfield: 11-Jul-2019 21:17:44

Channel:Brightfield set at:11-Jul-2019 21:17:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 21:17:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:17:45

Channel configuration set to: GFP: 11-Jul-2019 21:17:46

Channel:GFP set at:11-Jul-2019 21:17:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 21:17:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:17:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:17:48

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.225

Channel configuration set to: Brightfield: 11-Jul-2019 21:17:49

Channel:Brightfield set at:11-Jul-2019 21:17:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 21:17:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:17:50

Channel configuration set to: GFP: 11-Jul-2019 21:17:51

Channel:GFP set at:11-Jul-2019 21:17:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 21:17:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:17:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:17:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.4

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:17:55

Channel:Brightfield set at:11-Jul-2019 21:17:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 21:17:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:17:55

Channel configuration set to: GFP: 11-Jul-2019 21:17:56

Channel:GFP set at:11-Jul-2019 21:17:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 21:17:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:17:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:17:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:00

Channel:Brightfield set at:11-Jul-2019 21:18:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:18:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:00

Channel configuration set to: GFP: 11-Jul-2019 21:18:02

Channel:GFP set at:11-Jul-2019 21:18:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:18:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:05

Channel:Brightfield set at:11-Jul-2019 21:18:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 21:18:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:05

Channel configuration set to: GFP: 11-Jul-2019 21:18:06

Channel:GFP set at:11-Jul-2019 21:18:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 21:18:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:09

Channel:Brightfield set at:11-Jul-2019 21:18:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 21:18:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:10

Channel configuration set to: GFP: 11-Jul-2019 21:18:11

Channel:GFP set at:11-Jul-2019 21:18:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 21:18:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:11

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:13

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:14

Channel:Brightfield set at:11-Jul-2019 21:18:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 11-Jul-2019 21:18:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:14

Channel configuration set to: GFP: 11-Jul-2019 21:18:16

Channel:GFP set at:11-Jul-2019 21:18:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 21:18:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:16

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:18

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:19

Channel:Brightfield set at:11-Jul-2019 21:18:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 21:18:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:19

Channel configuration set to: GFP: 11-Jul-2019 21:18:21

Channel:GFP set at:11-Jul-2019 21:18:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 21:18:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:21

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:23

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:24

Channel:Brightfield set at:11-Jul-2019 21:18:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 21:18:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:24

Channel configuration set to: GFP: 11-Jul-2019 21:18:25

Channel:GFP set at:11-Jul-2019 21:18:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 21:18:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:26

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:27

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:29

Channel:Brightfield set at:11-Jul-2019 21:18:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 11-Jul-2019 21:18:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:29

Channel configuration set to: GFP: 11-Jul-2019 21:18:31

Channel:GFP set at:11-Jul-2019 21:18:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 11-Jul-2019 21:18:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:33

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:34

Channel:Brightfield set at:11-Jul-2019 21:18:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 21:18:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:35

Channel configuration set to: GFP: 11-Jul-2019 21:18:36

Channel:GFP set at:11-Jul-2019 21:18:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 21:18:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:36

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:38

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:39

Channel:Brightfield set at:11-Jul-2019 21:18:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 21:18:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:40

Channel configuration set to: GFP: 11-Jul-2019 21:18:41

Channel:GFP set at:11-Jul-2019 21:18:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 21:18:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:42

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:43

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:44

Channel:Brightfield set at:11-Jul-2019 21:18:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 21:18:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:45

Channel configuration set to: GFP: 11-Jul-2019 21:18:46

Channel:GFP set at:11-Jul-2019 21:18:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 21:18:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:46

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:48

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.6

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:50

Channel:Brightfield set at:11-Jul-2019 21:18:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 21:18:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:50

Channel configuration set to: GFP: 11-Jul-2019 21:18:51

Channel:GFP set at:11-Jul-2019 21:18:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 21:18:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:53

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:54

Channel:Brightfield set at:11-Jul-2019 21:18:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 21:18:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:55

Channel configuration set to: GFP: 11-Jul-2019 21:18:56

Channel:GFP set at:11-Jul-2019 21:18:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 21:18:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:18:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:18:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 11-Jul-2019 21:19:00

Channel:Brightfield set at:11-Jul-2019 21:19:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 21:19:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:19:00

Channel configuration set to: GFP: 11-Jul-2019 21:19:02

Channel:GFP set at:11-Jul-2019 21:19:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 21:19:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:19:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:59 completed at:11-Jul-2019 21:19:04

Time since start of timelapse:17490.9481

PFS is locked

Time to next time point:209.1569



------Time point_60------

Memory available for all arrays: 61102288896

Memory used by Matlab: 2208833536

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:34

Channel:Brightfield set at:11-Jul-2019 21:22:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 21:22:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:22:35

Channel configuration set to: GFP: 11-Jul-2019 21:22:36

Channel:GFP set at:11-Jul-2019 21:22:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 21:22:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:22:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:40

Channel:Brightfield set at:11-Jul-2019 21:22:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 21:22:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:22:40

Channel configuration set to: GFP: 11-Jul-2019 21:22:41

Channel:GFP set at:11-Jul-2019 21:22:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 21:22:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:22:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:43

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.875

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:45

Channel:Brightfield set at:11-Jul-2019 21:22:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 11-Jul-2019 21:22:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:22:45

Channel configuration set to: GFP: 11-Jul-2019 21:22:46

Channel:GFP set at:11-Jul-2019 21:22:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0751. 11-Jul-2019 21:22:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:22:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.55

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:49

Channel:Brightfield set at:11-Jul-2019 21:22:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 21:22:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:22:50

Channel configuration set to: GFP: 11-Jul-2019 21:22:51

Channel:GFP set at:11-Jul-2019 21:22:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 11-Jul-2019 21:22:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:22:51

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:53

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:54

Channel:Brightfield set at:11-Jul-2019 21:22:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 21:22:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:22:54

Channel configuration set to: GFP: 11-Jul-2019 21:22:56

Channel:GFP set at:11-Jul-2019 21:22:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 11-Jul-2019 21:22:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:22:56

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:58

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:22:59

Channel:Brightfield set at:11-Jul-2019 21:22:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:22:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:22:59

Channel configuration set to: GFP: 11-Jul-2019 21:23:01

Channel:GFP set at:11-Jul-2019 21:23:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:23:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:01

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:03

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:04

Channel:Brightfield set at:11-Jul-2019 21:23:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 21:23:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:05

Channel configuration set to: GFP: 11-Jul-2019 21:23:06

Channel:GFP set at:11-Jul-2019 21:23:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 21:23:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:06

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:08

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:10

Channel:Brightfield set at:11-Jul-2019 21:23:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 21:23:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:10

Channel configuration set to: GFP: 11-Jul-2019 21:23:12

Channel:GFP set at:11-Jul-2019 21:23:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 21:23:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:12

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:15

Channel:Brightfield set at:11-Jul-2019 21:23:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 11-Jul-2019 21:23:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:16

Channel configuration set to: GFP: 11-Jul-2019 21:23:17

Channel:GFP set at:11-Jul-2019 21:23:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 11-Jul-2019 21:23:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:17

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:19

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:20

Channel:Brightfield set at:11-Jul-2019 21:23:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 21:23:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:20

Channel configuration set to: GFP: 11-Jul-2019 21:23:22

Channel:GFP set at:11-Jul-2019 21:23:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 21:23:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:25

Channel:Brightfield set at:11-Jul-2019 21:23:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 21:23:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:25

Channel configuration set to: GFP: 11-Jul-2019 21:23:27

Channel:GFP set at:11-Jul-2019 21:23:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 21:23:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:31

Channel:Brightfield set at:11-Jul-2019 21:23:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 11-Jul-2019 21:23:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:31

Channel configuration set to: GFP: 11-Jul-2019 21:23:32

Channel:GFP set at:11-Jul-2019 21:23:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 11-Jul-2019 21:23:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:36

Channel:Brightfield set at:11-Jul-2019 21:23:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 21:23:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:36

Channel configuration set to: GFP: 11-Jul-2019 21:23:38

Channel:GFP set at:11-Jul-2019 21:23:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 21:23:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:41

Channel:Brightfield set at:11-Jul-2019 21:23:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 21:23:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:41

Channel configuration set to: GFP: 11-Jul-2019 21:23:43

Channel:GFP set at:11-Jul-2019 21:23:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 21:23:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:45

Channel:Brightfield set at:11-Jul-2019 21:23:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 21:23:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:46

Channel configuration set to: GFP: 11-Jul-2019 21:23:47

Channel:GFP set at:11-Jul-2019 21:23:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 21:23:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:51

Channel:Brightfield set at:11-Jul-2019 21:23:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 21:23:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:51

Channel configuration set to: GFP: 11-Jul-2019 21:23:53

Channel:GFP set at:11-Jul-2019 21:23:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 21:23:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:53

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:56

Channel:Brightfield set at:11-Jul-2019 21:23:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 21:23:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:56

Channel configuration set to: GFP: 11-Jul-2019 21:23:58

Channel:GFP set at:11-Jul-2019 21:23:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4501. 11-Jul-2019 21:23:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:23:58

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:23:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-4.925

Channel configuration set to: Brightfield: 11-Jul-2019 21:24:00

Channel:Brightfield set at:11-Jul-2019 21:24:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 11-Jul-2019 21:24:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:24:01

Channel configuration set to: GFP: 11-Jul-2019 21:24:02

Channel:GFP set at:11-Jul-2019 21:24:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 11-Jul-2019 21:24:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:24:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:60 completed at:11-Jul-2019 21:24:04

Time since start of timelapse:17791.3981

PFS is locked

Time to next time point:208.7084

Switching pumps at 11-Jul-2019 21:27:33. Fast infusion/withdrawal step: Rate=100. Volume=50

Pump switch complete at:11-Jul-2019 21:27:33

Dominant pump is now: COM6 pumping at 0ul/min

Dominant medium is now: 2% glucose in SC



------Time point_61------

Memory available for all arrays: 61022105600

Memory used by Matlab: 2208833536

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:27:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.025

Channel configuration set to: Brightfield: 11-Jul-2019 21:27:35

Channel:Brightfield set at:11-Jul-2019 21:27:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 21:27:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:27:35

Channel configuration set to: GFP: 11-Jul-2019 21:27:37

Channel:GFP set at:11-Jul-2019 21:27:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 21:27:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:27:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:27:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.4

Channel configuration set to: Brightfield: 11-Jul-2019 21:27:40

Channel:Brightfield set at:11-Jul-2019 21:27:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 21:27:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:27:40

Channel configuration set to: GFP: 11-Jul-2019 21:27:42

Channel:GFP set at:11-Jul-2019 21:27:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 21:27:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:27:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:27:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.225

Channel configuration set to: Brightfield: 11-Jul-2019 21:27:44

Channel:Brightfield set at:11-Jul-2019 21:27:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 21:27:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:27:45

Channel configuration set to: GFP: 11-Jul-2019 21:27:46

Channel:GFP set at:11-Jul-2019 21:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 11-Jul-2019 21:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:27:46

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:27:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.45

PFS not locked. Waiting for lock...

PFS took 0.400000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:27:50

Channel:Brightfield set at:11-Jul-2019 21:27:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 21:27:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:27:50

Channel configuration set to: GFP: 11-Jul-2019 21:27:51

Channel:GFP set at:11-Jul-2019 21:27:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 21:27:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:27:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:27:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.575

Channel configuration set to: Brightfield: 11-Jul-2019 21:27:55

Channel:Brightfield set at:11-Jul-2019 21:27:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 21:27:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:27:55

Channel configuration set to: GFP: 11-Jul-2019 21:27:57

Channel:GFP set at:11-Jul-2019 21:27:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 21:27:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:27:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:27:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:00

Channel:Brightfield set at:11-Jul-2019 21:28:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 21:28:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:01

Channel configuration set to: GFP: 11-Jul-2019 21:28:02

Channel:GFP set at:11-Jul-2019 21:28:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:05

Channel:Brightfield set at:11-Jul-2019 21:28:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 21:28:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:05

Channel configuration set to: GFP: 11-Jul-2019 21:28:07

Channel:GFP set at:11-Jul-2019 21:28:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 21:28:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:07

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:09

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:11

Channel:Brightfield set at:11-Jul-2019 21:28:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 21:28:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:11

Channel configuration set to: GFP: 11-Jul-2019 21:28:12

Channel:GFP set at:11-Jul-2019 21:28:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 21:28:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:16

Channel:Brightfield set at:11-Jul-2019 21:28:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 21:28:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:16

Channel configuration set to: GFP: 11-Jul-2019 21:28:18

Channel:GFP set at:11-Jul-2019 21:28:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 21:28:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:20

Channel:Brightfield set at:11-Jul-2019 21:28:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 21:28:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:21

Channel configuration set to: GFP: 11-Jul-2019 21:28:22

Channel:GFP set at:11-Jul-2019 21:28:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 21:28:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:22

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:24

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:25

Channel:Brightfield set at:11-Jul-2019 21:28:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 11-Jul-2019 21:28:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:25

Channel configuration set to: GFP: 11-Jul-2019 21:28:26

Channel:GFP set at:11-Jul-2019 21:28:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 11-Jul-2019 21:28:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:27

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:29

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:29

Channel:Brightfield set at:11-Jul-2019 21:28:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 21:28:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:30

Channel configuration set to: GFP: 11-Jul-2019 21:28:31

Channel:GFP set at:11-Jul-2019 21:28:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 11-Jul-2019 21:28:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:31

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:34

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:35

Channel:Brightfield set at:11-Jul-2019 21:28:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 21:28:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:36

Channel configuration set to: GFP: 11-Jul-2019 21:28:37

Channel:GFP set at:11-Jul-2019 21:28:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 21:28:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:37

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:39

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:40

Channel:Brightfield set at:11-Jul-2019 21:28:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 21:28:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:41

Channel configuration set to: GFP: 11-Jul-2019 21:28:42

Channel:GFP set at:11-Jul-2019 21:28:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 21:28:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:44

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.725

PFS not locked. Waiting for lock...

PFS took 0.300000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:45

Channel:Brightfield set at:11-Jul-2019 21:28:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 21:28:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:46

Channel configuration set to: GFP: 11-Jul-2019 21:28:47

Channel:GFP set at:11-Jul-2019 21:28:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8751. 11-Jul-2019 21:28:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:47

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:49

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:50

Channel:Brightfield set at:11-Jul-2019 21:28:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 11-Jul-2019 21:28:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:50

Channel configuration set to: GFP: 11-Jul-2019 21:28:52

Channel:GFP set at:11-Jul-2019 21:28:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 11-Jul-2019 21:28:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:52

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:54

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.275

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:55

Channel:Brightfield set at:11-Jul-2019 21:28:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 21:28:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:55

Channel configuration set to: GFP: 11-Jul-2019 21:28:57

Channel:GFP set at:11-Jul-2019 21:28:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 21:28:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:28:57

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:28:59

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 11-Jul-2019 21:29:00

Channel:Brightfield set at:11-Jul-2019 21:29:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 11-Jul-2019 21:29:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:29:00

Channel configuration set to: GFP: 11-Jul-2019 21:29:02

Channel:GFP set at:11-Jul-2019 21:29:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 11-Jul-2019 21:29:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:29:02

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:61 completed at:11-Jul-2019 21:29:04

Time since start of timelapse:18090.993

PFS is locked

Time to next time point:209.5805



------Time point_62------

Memory available for all arrays: 61062520832

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:32:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.775

Channel configuration set to: Brightfield: 11-Jul-2019 21:32:35

Channel:Brightfield set at:11-Jul-2019 21:32:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 21:32:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:32:35

Channel configuration set to: GFP: 11-Jul-2019 21:32:36

Channel:GFP set at:11-Jul-2019 21:32:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 21:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:32:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:32:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.075

Channel configuration set to: Brightfield: 11-Jul-2019 21:32:40

Channel:Brightfield set at:11-Jul-2019 21:32:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 21:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:32:41

Channel configuration set to: GFP: 11-Jul-2019 21:32:42

Channel:GFP set at:11-Jul-2019 21:32:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 21:32:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:32:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:32:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.875

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:32:45

Channel:Brightfield set at:11-Jul-2019 21:32:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 21:32:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:32:46

Channel configuration set to: GFP: 11-Jul-2019 21:32:47

Channel:GFP set at:11-Jul-2019 21:32:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 11-Jul-2019 21:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:32:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:32:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.925

Channel configuration set to: Brightfield: 11-Jul-2019 21:32:50

Channel:Brightfield set at:11-Jul-2019 21:32:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7751. 11-Jul-2019 21:32:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:32:51

Channel configuration set to: GFP: 11-Jul-2019 21:32:52

Channel:GFP set at:11-Jul-2019 21:32:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7751. 11-Jul-2019 21:32:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:32:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:32:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 21:32:56

Channel:Brightfield set at:11-Jul-2019 21:32:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 11-Jul-2019 21:32:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:32:56

Channel configuration set to: GFP: 11-Jul-2019 21:32:57

Channel:GFP set at:11-Jul-2019 21:32:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 11-Jul-2019 21:32:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:32:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:00

Channel:Brightfield set at:11-Jul-2019 21:33:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2001. 11-Jul-2019 21:33:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:01

Channel configuration set to: GFP: 11-Jul-2019 21:33:02

Channel:GFP set at:11-Jul-2019 21:33:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1751. 11-Jul-2019 21:33:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:06

Channel:Brightfield set at:11-Jul-2019 21:33:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 21:33:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:06

Channel configuration set to: GFP: 11-Jul-2019 21:33:08

Channel:GFP set at:11-Jul-2019 21:33:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 21:33:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:11

Channel:Brightfield set at:11-Jul-2019 21:33:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 21:33:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:11

Channel configuration set to: GFP: 11-Jul-2019 21:33:13

Channel:GFP set at:11-Jul-2019 21:33:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 21:33:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.7

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:16

Channel:Brightfield set at:11-Jul-2019 21:33:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 21:33:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:16

Channel configuration set to: GFP: 11-Jul-2019 21:33:18

Channel:GFP set at:11-Jul-2019 21:33:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 21:33:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:21

Channel:Brightfield set at:11-Jul-2019 21:33:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 11-Jul-2019 21:33:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:21

Channel configuration set to: GFP: 11-Jul-2019 21:33:22

Channel:GFP set at:11-Jul-2019 21:33:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 11-Jul-2019 21:33:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:26

Channel:Brightfield set at:11-Jul-2019 21:33:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 11-Jul-2019 21:33:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:26

Channel configuration set to: GFP: 11-Jul-2019 21:33:28

Channel:GFP set at:11-Jul-2019 21:33:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 11-Jul-2019 21:33:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:31

Channel:Brightfield set at:11-Jul-2019 21:33:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 21:33:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:31

Channel configuration set to: GFP: 11-Jul-2019 21:33:32

Channel:GFP set at:11-Jul-2019 21:33:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 21:33:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:36

Channel:Brightfield set at:11-Jul-2019 21:33:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:33:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:37

Channel configuration set to: GFP: 11-Jul-2019 21:33:38

Channel:GFP set at:11-Jul-2019 21:33:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:33:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:42

Channel:Brightfield set at:11-Jul-2019 21:33:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8751. 11-Jul-2019 21:33:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:42

Channel configuration set to: GFP: 11-Jul-2019 21:33:43

Channel:GFP set at:11-Jul-2019 21:33:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 21:33:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:47

Channel:Brightfield set at:11-Jul-2019 21:33:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 11-Jul-2019 21:33:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:47

Channel configuration set to: GFP: 11-Jul-2019 21:33:48

Channel:GFP set at:11-Jul-2019 21:33:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 11-Jul-2019 21:33:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.225

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:52

Channel:Brightfield set at:11-Jul-2019 21:33:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 21:33:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:52

Channel configuration set to: GFP: 11-Jul-2019 21:33:53

Channel:GFP set at:11-Jul-2019 21:33:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 21:33:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 11-Jul-2019 21:33:56

Channel:Brightfield set at:11-Jul-2019 21:33:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 21:33:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:57

Channel configuration set to: GFP: 11-Jul-2019 21:33:58

Channel:GFP set at:11-Jul-2019 21:33:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 11-Jul-2019 21:33:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:33:58

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:34:00

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 21:34:02

Channel:Brightfield set at:11-Jul-2019 21:34:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 11-Jul-2019 21:34:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:34:02

Channel configuration set to: GFP: 11-Jul-2019 21:34:03

Channel:GFP set at:11-Jul-2019 21:34:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 21:34:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:34:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:62 completed at:11-Jul-2019 21:34:06

Time since start of timelapse:18392.1894

PFS is locked

Time to next time point:208.3856



------Time point_63------

Memory available for all arrays: 61097308160

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:37:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.65

Channel configuration set to: Brightfield: 11-Jul-2019 21:37:36

Channel:Brightfield set at:11-Jul-2019 21:37:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 11-Jul-2019 21:37:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:37:36

Channel configuration set to: GFP: 11-Jul-2019 21:37:37

Channel:GFP set at:11-Jul-2019 21:37:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 21:37:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:37:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:37:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.95

Channel configuration set to: Brightfield: 11-Jul-2019 21:37:41

Channel:Brightfield set at:11-Jul-2019 21:37:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 11-Jul-2019 21:37:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:37:41

Channel configuration set to: GFP: 11-Jul-2019 21:37:42

Channel:GFP set at:11-Jul-2019 21:37:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 11-Jul-2019 21:37:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:37:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:37:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.725

Channel configuration set to: Brightfield: 11-Jul-2019 21:37:46

Channel:Brightfield set at:11-Jul-2019 21:37:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1001. 11-Jul-2019 21:37:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:37:46

Channel configuration set to: GFP: 11-Jul-2019 21:37:47

Channel:GFP set at:11-Jul-2019 21:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1001. 11-Jul-2019 21:37:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:37:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:37:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 11-Jul-2019 21:37:51

Channel:Brightfield set at:11-Jul-2019 21:37:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 21:37:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:37:51

Channel configuration set to: GFP: 11-Jul-2019 21:37:52

Channel:GFP set at:11-Jul-2019 21:37:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 21:37:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:37:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:37:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 11-Jul-2019 21:37:56

Channel:Brightfield set at:11-Jul-2019 21:37:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 21:37:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:37:56

Channel configuration set to: GFP: 11-Jul-2019 21:37:57

Channel:GFP set at:11-Jul-2019 21:37:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 21:37:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:37:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:37:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.875

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:01

Channel:Brightfield set at:11-Jul-2019 21:38:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 21:38:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:01

Channel configuration set to: GFP: 11-Jul-2019 21:38:02

Channel:GFP set at:11-Jul-2019 21:38:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 21:38:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:06

Channel:Brightfield set at:11-Jul-2019 21:38:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 21:38:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:06

Channel configuration set to: GFP: 11-Jul-2019 21:38:08

Channel:GFP set at:11-Jul-2019 21:38:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 21:38:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:11

Channel:Brightfield set at:11-Jul-2019 21:38:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 21:38:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:11

Channel configuration set to: GFP: 11-Jul-2019 21:38:13

Channel:GFP set at:11-Jul-2019 21:38:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 21:38:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:16

Channel:Brightfield set at:11-Jul-2019 21:38:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:38:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:16

Channel configuration set to: GFP: 11-Jul-2019 21:38:18

Channel:GFP set at:11-Jul-2019 21:38:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 21:38:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:21

Channel:Brightfield set at:11-Jul-2019 21:38:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1001. 11-Jul-2019 21:38:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:21

Channel configuration set to: GFP: 11-Jul-2019 21:38:22

Channel:GFP set at:11-Jul-2019 21:38:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1001. 11-Jul-2019 21:38:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:26

Channel:Brightfield set at:11-Jul-2019 21:38:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5001. 11-Jul-2019 21:38:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:26

Channel configuration set to: GFP: 11-Jul-2019 21:38:28

Channel:GFP set at:11-Jul-2019 21:38:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5001. 11-Jul-2019 21:38:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:32

Channel:Brightfield set at:11-Jul-2019 21:38:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 11-Jul-2019 21:38:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:32

Channel configuration set to: GFP: 11-Jul-2019 21:38:33

Channel:GFP set at:11-Jul-2019 21:38:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 11-Jul-2019 21:38:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:37

Channel:Brightfield set at:11-Jul-2019 21:38:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 21:38:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:37

Channel configuration set to: GFP: 11-Jul-2019 21:38:39

Channel:GFP set at:11-Jul-2019 21:38:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 21:38:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:42

Channel:Brightfield set at:11-Jul-2019 21:38:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 21:38:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:43

Channel configuration set to: GFP: 11-Jul-2019 21:38:44

Channel:GFP set at:11-Jul-2019 21:38:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 21:38:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.375

PFS not locked. Waiting for lock...

PFS took 0.450000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:47

Channel:Brightfield set at:11-Jul-2019 21:38:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1751. 11-Jul-2019 21:38:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:48

Channel configuration set to: GFP: 11-Jul-2019 21:38:49

Channel:GFP set at:11-Jul-2019 21:38:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1751. 11-Jul-2019 21:38:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.2

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:52

Channel:Brightfield set at:11-Jul-2019 21:38:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 11-Jul-2019 21:38:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:53

Channel configuration set to: GFP: 11-Jul-2019 21:38:54

Channel:GFP set at:11-Jul-2019 21:38:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 11-Jul-2019 21:38:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.95

Channel configuration set to: Brightfield: 11-Jul-2019 21:38:58

Channel:Brightfield set at:11-Jul-2019 21:38:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 21:38:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:38:58

Channel configuration set to: GFP: 11-Jul-2019 21:38:59

Channel:GFP set at:11-Jul-2019 21:38:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 21:39:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:39:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:39:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 21:39:02

Channel:Brightfield set at:11-Jul-2019 21:39:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 21:39:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:39:03

Channel configuration set to: GFP: 11-Jul-2019 21:39:04

Channel:GFP set at:11-Jul-2019 21:39:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 11-Jul-2019 21:39:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:39:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:63 completed at:11-Jul-2019 21:39:06

Time since start of timelapse:18692.6204

PFS is locked

Time to next time point:207.9561



------Time point_64------

Memory available for all arrays: 61096443904

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:42:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 11-Jul-2019 21:42:36

Channel:Brightfield set at:11-Jul-2019 21:42:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 21:42:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:42:36

Channel configuration set to: GFP: 11-Jul-2019 21:42:38

Channel:GFP set at:11-Jul-2019 21:42:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 21:42:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:42:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:42:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.85

Channel configuration set to: Brightfield: 11-Jul-2019 21:42:41

Channel:Brightfield set at:11-Jul-2019 21:42:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 11-Jul-2019 21:42:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:42:41

Channel configuration set to: GFP: 11-Jul-2019 21:42:43

Channel:GFP set at:11-Jul-2019 21:42:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 21:42:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:42:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:42:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 11-Jul-2019 21:42:45

Channel:Brightfield set at:11-Jul-2019 21:42:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1501. 11-Jul-2019 21:42:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:42:46

Channel configuration set to: GFP: 11-Jul-2019 21:42:47

Channel:GFP set at:11-Jul-2019 21:42:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1501. 11-Jul-2019 21:42:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:42:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:42:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.8

Channel configuration set to: Brightfield: 11-Jul-2019 21:42:51

Channel:Brightfield set at:11-Jul-2019 21:42:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 21:42:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:42:51

Channel configuration set to: GFP: 11-Jul-2019 21:42:52

Channel:GFP set at:11-Jul-2019 21:42:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 21:42:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:42:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:42:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 11-Jul-2019 21:42:56

Channel:Brightfield set at:11-Jul-2019 21:42:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 21:42:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:42:56

Channel configuration set to: GFP: 11-Jul-2019 21:42:57

Channel:GFP set at:11-Jul-2019 21:42:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 21:42:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:42:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:42:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.8

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:01

Channel:Brightfield set at:11-Jul-2019 21:43:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 11-Jul-2019 21:43:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:01

Channel configuration set to: GFP: 11-Jul-2019 21:43:03

Channel:GFP set at:11-Jul-2019 21:43:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 21:43:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:06

Channel:Brightfield set at:11-Jul-2019 21:43:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 21:43:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:06

Channel configuration set to: GFP: 11-Jul-2019 21:43:07

Channel:GFP set at:11-Jul-2019 21:43:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 11-Jul-2019 21:43:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:11

Channel:Brightfield set at:11-Jul-2019 21:43:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 11-Jul-2019 21:43:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:12

Channel configuration set to: GFP: 11-Jul-2019 21:43:13

Channel:GFP set at:11-Jul-2019 21:43:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 11-Jul-2019 21:43:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:16

Channel:Brightfield set at:11-Jul-2019 21:43:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 21:43:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:16

Channel configuration set to: GFP: 11-Jul-2019 21:43:18

Channel:GFP set at:11-Jul-2019 21:43:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 11-Jul-2019 21:43:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:21

Channel:Brightfield set at:11-Jul-2019 21:43:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2501. 11-Jul-2019 21:43:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:22

Channel configuration set to: GFP: 11-Jul-2019 21:43:23

Channel:GFP set at:11-Jul-2019 21:43:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2501. 11-Jul-2019 21:43:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:26

Channel:Brightfield set at:11-Jul-2019 21:43:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 11-Jul-2019 21:43:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:26

Channel configuration set to: GFP: 11-Jul-2019 21:43:28

Channel:GFP set at:11-Jul-2019 21:43:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 11-Jul-2019 21:43:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:31

Channel:Brightfield set at:11-Jul-2019 21:43:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 21:43:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:32

Channel configuration set to: GFP: 11-Jul-2019 21:43:33

Channel:GFP set at:11-Jul-2019 21:43:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 21:43:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:37

Channel:Brightfield set at:11-Jul-2019 21:43:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 21:43:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:37

Channel configuration set to: GFP: 11-Jul-2019 21:43:38

Channel:GFP set at:11-Jul-2019 21:43:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 21:43:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:42

Channel:Brightfield set at:11-Jul-2019 21:43:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 21:43:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:42

Channel configuration set to: GFP: 11-Jul-2019 21:43:43

Channel:GFP set at:11-Jul-2019 21:43:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 21:43:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:46

Channel:Brightfield set at:11-Jul-2019 21:43:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 21:43:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:47

Channel configuration set to: GFP: 11-Jul-2019 21:43:48

Channel:GFP set at:11-Jul-2019 21:43:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 21:43:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:48

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:51

Channel:Brightfield set at:11-Jul-2019 21:43:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 21:43:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:51

Channel configuration set to: GFP: 11-Jul-2019 21:43:53

Channel:GFP set at:11-Jul-2019 21:43:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 21:43:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:53

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.8

Channel configuration set to: Brightfield: 11-Jul-2019 21:43:56

Channel:Brightfield set at:11-Jul-2019 21:43:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 11-Jul-2019 21:43:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:57

Channel configuration set to: GFP: 11-Jul-2019 21:43:58

Channel:GFP set at:11-Jul-2019 21:43:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 21:43:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:43:58

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:44:00

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 21:44:01

Channel:Brightfield set at:11-Jul-2019 21:44:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 21:44:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:44:02

Channel configuration set to: GFP: 11-Jul-2019 21:44:03

Channel:GFP set at:11-Jul-2019 21:44:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 21:44:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:44:03

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:64 completed at:11-Jul-2019 21:44:05

Time since start of timelapse:18991.277

PFS is locked

Time to next time point:209.301



------Time point_65------

Memory available for all arrays: 61086932992

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:47:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 11-Jul-2019 21:47:36

Channel:Brightfield set at:11-Jul-2019 21:47:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 21:47:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:47:36

Channel configuration set to: GFP: 11-Jul-2019 21:47:38

Channel:GFP set at:11-Jul-2019 21:47:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 21:47:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:47:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:47:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.7

Channel configuration set to: Brightfield: 11-Jul-2019 21:47:41

Channel:Brightfield set at:11-Jul-2019 21:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 21:47:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:47:41

Channel configuration set to: GFP: 11-Jul-2019 21:47:43

Channel:GFP set at:11-Jul-2019 21:47:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 11-Jul-2019 21:47:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:47:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:47:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 11-Jul-2019 21:47:46

Channel:Brightfield set at:11-Jul-2019 21:47:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3001. 11-Jul-2019 21:47:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:47:46

Channel configuration set to: GFP: 11-Jul-2019 21:47:48

Channel:GFP set at:11-Jul-2019 21:47:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.3001. 11-Jul-2019 21:47:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:47:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:47:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.675

Channel configuration set to: Brightfield: 11-Jul-2019 21:47:51

Channel:Brightfield set at:11-Jul-2019 21:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9501. 11-Jul-2019 21:47:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:47:52

Channel configuration set to: GFP: 11-Jul-2019 21:47:53

Channel:GFP set at:11-Jul-2019 21:47:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 21:47:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:47:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:47:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 11-Jul-2019 21:47:56

Channel:Brightfield set at:11-Jul-2019 21:47:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 21:47:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:47:57

Channel configuration set to: GFP: 11-Jul-2019 21:47:58

Channel:GFP set at:11-Jul-2019 21:47:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 21:47:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:47:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:01

Channel:Brightfield set at:11-Jul-2019 21:48:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 21:48:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:01

Channel configuration set to: GFP: 11-Jul-2019 21:48:03

Channel:GFP set at:11-Jul-2019 21:48:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 21:48:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:06

Channel:Brightfield set at:11-Jul-2019 21:48:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 21:48:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:06

Channel configuration set to: GFP: 11-Jul-2019 21:48:08

Channel:GFP set at:11-Jul-2019 21:48:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 21:48:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:12

Channel:Brightfield set at:11-Jul-2019 21:48:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 11-Jul-2019 21:48:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:12

Channel configuration set to: GFP: 11-Jul-2019 21:48:14

Channel:GFP set at:11-Jul-2019 21:48:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 11-Jul-2019 21:48:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:17

Channel:Brightfield set at:11-Jul-2019 21:48:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 11-Jul-2019 21:48:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:18

Channel configuration set to: GFP: 11-Jul-2019 21:48:19

Channel:GFP set at:11-Jul-2019 21:48:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 11-Jul-2019 21:48:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:22

Channel:Brightfield set at:11-Jul-2019 21:48:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 11-Jul-2019 21:48:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:23

Channel configuration set to: GFP: 11-Jul-2019 21:48:24

Channel:GFP set at:11-Jul-2019 21:48:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3501. 11-Jul-2019 21:48:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:27

Channel:Brightfield set at:11-Jul-2019 21:48:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 11-Jul-2019 21:48:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:27

Channel configuration set to: GFP: 11-Jul-2019 21:48:29

Channel:GFP set at:11-Jul-2019 21:48:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 11-Jul-2019 21:48:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:33

Channel:Brightfield set at:11-Jul-2019 21:48:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 11-Jul-2019 21:48:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:33

Channel configuration set to: GFP: 11-Jul-2019 21:48:34

Channel:GFP set at:11-Jul-2019 21:48:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 21:48:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:38

Channel:Brightfield set at:11-Jul-2019 21:48:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 21:48:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:38

Channel configuration set to: GFP: 11-Jul-2019 21:48:39

Channel:GFP set at:11-Jul-2019 21:48:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 21:48:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.825

PFS not locked. Waiting for lock...

PFS took 0.250000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:43

Channel:Brightfield set at:11-Jul-2019 21:48:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 21:48:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:44

Channel configuration set to: GFP: 11-Jul-2019 21:48:45

Channel:GFP set at:11-Jul-2019 21:48:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 21:48:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:48

Channel:Brightfield set at:11-Jul-2019 21:48:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 21:48:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:48

Channel configuration set to: GFP: 11-Jul-2019 21:48:50

Channel:GFP set at:11-Jul-2019 21:48:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 21:48:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.1

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:53

Channel:Brightfield set at:11-Jul-2019 21:48:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 21:48:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:53

Channel configuration set to: GFP: 11-Jul-2019 21:48:55

Channel:GFP set at:11-Jul-2019 21:48:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 21:48:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.475

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:48:58

Channel:Brightfield set at:11-Jul-2019 21:48:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 21:48:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:48:59

Channel configuration set to: GFP: 11-Jul-2019 21:49:00

Channel:GFP set at:11-Jul-2019 21:49:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 11-Jul-2019 21:49:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:49:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:49:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 21:49:03

Channel:Brightfield set at:11-Jul-2019 21:49:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 21:49:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:49:04

Channel configuration set to: GFP: 11-Jul-2019 21:49:05

Channel:GFP set at:11-Jul-2019 21:49:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 21:49:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:49:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:65 completed at:11-Jul-2019 21:49:07

Time since start of timelapse:19293.3317

PFS is locked

Time to next time point:207.2478



------Time point_66------

Memory available for all arrays: 61095464960

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:52:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 11-Jul-2019 21:52:36

Channel:Brightfield set at:11-Jul-2019 21:52:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 11-Jul-2019 21:52:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:52:36

Channel configuration set to: GFP: 11-Jul-2019 21:52:38

Channel:GFP set at:11-Jul-2019 21:52:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 11-Jul-2019 21:52:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:52:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:52:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.65

Channel configuration set to: Brightfield: 11-Jul-2019 21:52:41

Channel:Brightfield set at:11-Jul-2019 21:52:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 11-Jul-2019 21:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:52:41

Channel configuration set to: GFP: 11-Jul-2019 21:52:43

Channel:GFP set at:11-Jul-2019 21:52:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 11-Jul-2019 21:52:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:52:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:52:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.175

Channel configuration set to: Brightfield: 11-Jul-2019 21:52:46

Channel:Brightfield set at:11-Jul-2019 21:52:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 21:52:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:52:47

Channel configuration set to: GFP: 11-Jul-2019 21:52:48

Channel:GFP set at:11-Jul-2019 21:52:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 21:52:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:52:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:52:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.6

Channel configuration set to: Brightfield: 11-Jul-2019 21:52:51

Channel:Brightfield set at:11-Jul-2019 21:52:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0501. 11-Jul-2019 21:52:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:52:52

Channel configuration set to: GFP: 11-Jul-2019 21:52:53

Channel:GFP set at:11-Jul-2019 21:52:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 21:52:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:52:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:52:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 11-Jul-2019 21:52:57

Channel:Brightfield set at:11-Jul-2019 21:52:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 21:52:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:52:57

Channel configuration set to: GFP: 11-Jul-2019 21:52:58

Channel:GFP set at:11-Jul-2019 21:52:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 21:52:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:52:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.025

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:01

Channel:Brightfield set at:11-Jul-2019 21:53:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 21:53:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:02

Channel configuration set to: GFP: 11-Jul-2019 21:53:03

Channel:GFP set at:11-Jul-2019 21:53:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 21:53:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:07

Channel:Brightfield set at:11-Jul-2019 21:53:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 21:53:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:07

Channel configuration set to: GFP: 11-Jul-2019 21:53:09

Channel:GFP set at:11-Jul-2019 21:53:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 21:53:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:11

Channel:Brightfield set at:11-Jul-2019 21:53:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 21:53:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:12

Channel configuration set to: GFP: 11-Jul-2019 21:53:13

Channel:GFP set at:11-Jul-2019 21:53:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 21:53:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.3

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:17

Channel:Brightfield set at:11-Jul-2019 21:53:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 21:53:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:17

Channel configuration set to: GFP: 11-Jul-2019 21:53:19

Channel:GFP set at:11-Jul-2019 21:53:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 11-Jul-2019 21:53:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:22

Channel:Brightfield set at:11-Jul-2019 21:53:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 11-Jul-2019 21:53:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:22

Channel configuration set to: GFP: 11-Jul-2019 21:53:23

Channel:GFP set at:11-Jul-2019 21:53:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 11-Jul-2019 21:53:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:26

Channel:Brightfield set at:11-Jul-2019 21:53:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 21:53:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:27

Channel configuration set to: GFP: 11-Jul-2019 21:53:28

Channel:GFP set at:11-Jul-2019 21:53:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 21:53:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:31

Channel:Brightfield set at:11-Jul-2019 21:53:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 11-Jul-2019 21:53:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:32

Channel configuration set to: GFP: 11-Jul-2019 21:53:33

Channel:GFP set at:11-Jul-2019 21:53:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 11-Jul-2019 21:53:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:37

Channel:Brightfield set at:11-Jul-2019 21:53:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 21:53:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:37

Channel configuration set to: GFP: 11-Jul-2019 21:53:38

Channel:GFP set at:11-Jul-2019 21:53:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 21:53:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.6

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:42

Channel:Brightfield set at:11-Jul-2019 21:53:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 21:53:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:42

Channel configuration set to: GFP: 11-Jul-2019 21:53:43

Channel:GFP set at:11-Jul-2019 21:53:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 21:53:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:47

Channel:Brightfield set at:11-Jul-2019 21:53:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 21:53:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:47

Channel configuration set to: GFP: 11-Jul-2019 21:53:48

Channel:GFP set at:11-Jul-2019 21:53:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 21:53:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-4.95

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:52

Channel:Brightfield set at:11-Jul-2019 21:53:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 21:53:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:52

Channel configuration set to: GFP: 11-Jul-2019 21:53:53

Channel:GFP set at:11-Jul-2019 21:53:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 11-Jul-2019 21:53:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.55

Channel configuration set to: Brightfield: 11-Jul-2019 21:53:57

Channel:Brightfield set at:11-Jul-2019 21:53:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1501. 11-Jul-2019 21:53:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:57

Channel configuration set to: GFP: 11-Jul-2019 21:53:58

Channel:GFP set at:11-Jul-2019 21:53:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1501. 11-Jul-2019 21:53:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:53:59

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:54:00

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 21:54:02

Channel:Brightfield set at:11-Jul-2019 21:54:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1251. 11-Jul-2019 21:54:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:54:02

Channel configuration set to: GFP: 11-Jul-2019 21:54:03

Channel:GFP set at:11-Jul-2019 21:54:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1251. 11-Jul-2019 21:54:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:54:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:66 completed at:11-Jul-2019 21:54:05

Time since start of timelapse:19591.6166

PFS is locked

Time to next time point:208.9644



------Time point_67------

Memory available for all arrays: 61097295872

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:57:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.475

Channel configuration set to: Brightfield: 11-Jul-2019 21:57:36

Channel:Brightfield set at:11-Jul-2019 21:57:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 11-Jul-2019 21:57:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:57:36

Channel configuration set to: GFP: 11-Jul-2019 21:57:38

Channel:GFP set at:11-Jul-2019 21:57:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 11-Jul-2019 21:57:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:57:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:57:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.775

Channel configuration set to: Brightfield: 11-Jul-2019 21:57:41

Channel:Brightfield set at:11-Jul-2019 21:57:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0751. 11-Jul-2019 21:57:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:57:41

Channel configuration set to: GFP: 11-Jul-2019 21:57:43

Channel:GFP set at:11-Jul-2019 21:57:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0751. 11-Jul-2019 21:57:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:57:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:57:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 11-Jul-2019 21:57:46

Channel:Brightfield set at:11-Jul-2019 21:57:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2251. 11-Jul-2019 21:57:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:57:46

Channel configuration set to: GFP: 11-Jul-2019 21:57:47

Channel:GFP set at:11-Jul-2019 21:57:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2251. 11-Jul-2019 21:57:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:57:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:57:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 11-Jul-2019 21:57:51

Channel:Brightfield set at:11-Jul-2019 21:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 21:57:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:57:51

Channel configuration set to: GFP: 11-Jul-2019 21:57:52

Channel:GFP set at:11-Jul-2019 21:57:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 21:57:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:57:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:57:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 11-Jul-2019 21:57:56

Channel:Brightfield set at:11-Jul-2019 21:57:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8751. 11-Jul-2019 21:57:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:57:56

Channel configuration set to: GFP: 11-Jul-2019 21:57:58

Channel:GFP set at:11-Jul-2019 21:57:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8751. 11-Jul-2019 21:57:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:57:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:01

Channel:Brightfield set at:11-Jul-2019 21:58:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 21:58:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:01

Channel configuration set to: GFP: 11-Jul-2019 21:58:02

Channel:GFP set at:11-Jul-2019 21:58:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 11-Jul-2019 21:58:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:06

Channel:Brightfield set at:11-Jul-2019 21:58:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 21:58:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:06

Channel configuration set to: GFP: 11-Jul-2019 21:58:07

Channel:GFP set at:11-Jul-2019 21:58:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 21:58:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:11

Channel:Brightfield set at:11-Jul-2019 21:58:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 21:58:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:11

Channel configuration set to: GFP: 11-Jul-2019 21:58:12

Channel:GFP set at:11-Jul-2019 21:58:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 21:58:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:16

Channel:Brightfield set at:11-Jul-2019 21:58:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 21:58:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:16

Channel configuration set to: GFP: 11-Jul-2019 21:58:18

Channel:GFP set at:11-Jul-2019 21:58:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 21:58:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:21

Channel:Brightfield set at:11-Jul-2019 21:58:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2751. 11-Jul-2019 21:58:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:21

Channel configuration set to: GFP: 11-Jul-2019 21:58:23

Channel:GFP set at:11-Jul-2019 21:58:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2501. 11-Jul-2019 21:58:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.375

PFS not locked. Waiting for lock...

PFS took 0.250000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:26

Channel:Brightfield set at:11-Jul-2019 21:58:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 11-Jul-2019 21:58:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:26

Channel configuration set to: GFP: 11-Jul-2019 21:58:27

Channel:GFP set at:11-Jul-2019 21:58:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 11-Jul-2019 21:58:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:30

Channel:Brightfield set at:11-Jul-2019 21:58:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 11-Jul-2019 21:58:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:31

Channel configuration set to: GFP: 11-Jul-2019 21:58:32

Channel:GFP set at:11-Jul-2019 21:58:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 11-Jul-2019 21:58:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:36

Channel:Brightfield set at:11-Jul-2019 21:58:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 21:58:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:37

Channel configuration set to: GFP: 11-Jul-2019 21:58:38

Channel:GFP set at:11-Jul-2019 21:58:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 21:58:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:38

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:40

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:41

Channel:Brightfield set at:11-Jul-2019 21:58:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 21:58:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:42

Channel configuration set to: GFP: 11-Jul-2019 21:58:43

Channel:GFP set at:11-Jul-2019 21:58:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 21:58:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:45

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:46

Channel:Brightfield set at:11-Jul-2019 21:58:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 21:58:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:47

Channel configuration set to: GFP: 11-Jul-2019 21:58:48

Channel:GFP set at:11-Jul-2019 21:58:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 21:58:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:48

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.1

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:51

Channel:Brightfield set at:11-Jul-2019 21:58:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 21:58:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:52

Channel configuration set to: GFP: 11-Jul-2019 21:58:53

Channel:GFP set at:11-Jul-2019 21:58:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 21:58:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:53

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.825

Channel configuration set to: Brightfield: 11-Jul-2019 21:58:56

Channel:Brightfield set at:11-Jul-2019 21:58:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 21:58:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:57

Channel configuration set to: GFP: 11-Jul-2019 21:58:58

Channel:GFP set at:11-Jul-2019 21:58:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 11-Jul-2019 21:58:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:58:58

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 21:59:00

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 11-Jul-2019 21:59:01

Channel:Brightfield set at:11-Jul-2019 21:59:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 21:59:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:59:02

Channel configuration set to: GFP: 11-Jul-2019 21:59:03

Channel:GFP set at:11-Jul-2019 21:59:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 11-Jul-2019 21:59:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 21:59:03

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:67 completed at:11-Jul-2019 21:59:05

Time since start of timelapse:19891.2738

PFS is locked

Time to next time point:209.3087



------Time point_68------

Memory available for all arrays: 61097435136

Memory used by Matlab: 2209095680

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:02:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 11-Jul-2019 22:02:36

Channel:Brightfield set at:11-Jul-2019 22:02:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 22:02:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:02:36

Channel configuration set to: GFP: 11-Jul-2019 22:02:38

Channel:GFP set at:11-Jul-2019 22:02:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 22:02:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:02:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:02:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.9

Channel configuration set to: Brightfield: 11-Jul-2019 22:02:41

Channel:Brightfield set at:11-Jul-2019 22:02:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 22:02:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:02:41

Channel configuration set to: GFP: 11-Jul-2019 22:02:43

Channel:GFP set at:11-Jul-2019 22:02:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 22:02:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:02:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:02:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.425

Channel configuration set to: Brightfield: 11-Jul-2019 22:02:46

Channel:Brightfield set at:11-Jul-2019 22:02:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 22:02:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:02:47

Channel configuration set to: GFP: 11-Jul-2019 22:02:48

Channel:GFP set at:11-Jul-2019 22:02:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 22:02:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:02:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:02:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8

PFS not locked. Waiting for lock...

PFS took 0.250000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:02:51

Channel:Brightfield set at:11-Jul-2019 22:02:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 11-Jul-2019 22:02:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:02:52

Channel configuration set to: GFP: 11-Jul-2019 22:02:53

Channel:GFP set at:11-Jul-2019 22:02:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 11-Jul-2019 22:02:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:02:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:02:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 11-Jul-2019 22:02:56

Channel:Brightfield set at:11-Jul-2019 22:02:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 22:02:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:02:57

Channel configuration set to: GFP: 11-Jul-2019 22:02:58

Channel:GFP set at:11-Jul-2019 22:02:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 22:02:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:02:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.775

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:02

Channel:Brightfield set at:11-Jul-2019 22:03:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 22:03:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:02

Channel configuration set to: GFP: 11-Jul-2019 22:03:04

Channel:GFP set at:11-Jul-2019 22:03:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 11-Jul-2019 22:03:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:07

Channel:Brightfield set at:11-Jul-2019 22:03:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 22:03:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:07

Channel configuration set to: GFP: 11-Jul-2019 22:03:09

Channel:GFP set at:11-Jul-2019 22:03:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 22:03:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:12

Channel:Brightfield set at:11-Jul-2019 22:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 22:03:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:13

Channel configuration set to: GFP: 11-Jul-2019 22:03:14

Channel:GFP set at:11-Jul-2019 22:03:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 11-Jul-2019 22:03:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:17

Channel:Brightfield set at:11-Jul-2019 22:03:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 11-Jul-2019 22:03:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:17

Channel configuration set to: GFP: 11-Jul-2019 22:03:18

Channel:GFP set at:11-Jul-2019 22:03:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0001. 11-Jul-2019 22:03:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:22

Channel:Brightfield set at:11-Jul-2019 22:03:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2001. 11-Jul-2019 22:03:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:22

Channel configuration set to: GFP: 11-Jul-2019 22:03:24

Channel:GFP set at:11-Jul-2019 22:03:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2251. 11-Jul-2019 22:03:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:27

Channel:Brightfield set at:11-Jul-2019 22:03:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 22:03:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:27

Channel configuration set to: GFP: 11-Jul-2019 22:03:28

Channel:GFP set at:11-Jul-2019 22:03:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 22:03:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:32

Channel:Brightfield set at:11-Jul-2019 22:03:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 22:03:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:33

Channel configuration set to: GFP: 11-Jul-2019 22:03:34

Channel:GFP set at:11-Jul-2019 22:03:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 22:03:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:37

Channel:Brightfield set at:11-Jul-2019 22:03:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 22:03:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:38

Channel configuration set to: GFP: 11-Jul-2019 22:03:39

Channel:GFP set at:11-Jul-2019 22:03:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 22:03:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:43

Channel:Brightfield set at:11-Jul-2019 22:03:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 22:03:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:43

Channel configuration set to: GFP: 11-Jul-2019 22:03:44

Channel:GFP set at:11-Jul-2019 22:03:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 22:03:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:48

Channel:Brightfield set at:11-Jul-2019 22:03:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 22:03:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:48

Channel configuration set to: GFP: 11-Jul-2019 22:03:49

Channel:GFP set at:11-Jul-2019 22:03:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 11-Jul-2019 22:03:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:52

Channel:Brightfield set at:11-Jul-2019 22:03:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 22:03:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:52

Channel configuration set to: GFP: 11-Jul-2019 22:03:54

Channel:GFP set at:11-Jul-2019 22:03:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 22:03:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.925

Channel configuration set to: Brightfield: 11-Jul-2019 22:03:57

Channel:Brightfield set at:11-Jul-2019 22:03:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 11-Jul-2019 22:03:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:58

Channel configuration set to: GFP: 11-Jul-2019 22:03:59

Channel:GFP set at:11-Jul-2019 22:03:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 11-Jul-2019 22:03:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:03:59

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:04:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 22:04:02

Channel:Brightfield set at:11-Jul-2019 22:04:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 22:04:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:04:03

Channel configuration set to: GFP: 11-Jul-2019 22:04:04

Channel:GFP set at:11-Jul-2019 22:04:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 11-Jul-2019 22:04:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:04:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:68 completed at:11-Jul-2019 22:04:06

Time since start of timelapse:20192.3185

PFS is locked

Time to next time point:208.2654



------Time point_69------

Memory available for all arrays: 61096669184

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:07:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.75

Channel configuration set to: Brightfield: 11-Jul-2019 22:07:36

Channel:Brightfield set at:11-Jul-2019 22:07:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 22:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:07:36

Channel configuration set to: GFP: 11-Jul-2019 22:07:38

Channel:GFP set at:11-Jul-2019 22:07:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 22:07:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:07:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:07:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.425

Channel configuration set to: Brightfield: 11-Jul-2019 22:07:41

Channel:Brightfield set at:11-Jul-2019 22:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 22:07:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:07:41

Channel configuration set to: GFP: 11-Jul-2019 22:07:42

Channel:GFP set at:11-Jul-2019 22:07:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 22:07:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:07:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:07:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 11-Jul-2019 22:07:46

Channel:Brightfield set at:11-Jul-2019 22:07:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 22:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:07:46

Channel configuration set to: GFP: 11-Jul-2019 22:07:48

Channel:GFP set at:11-Jul-2019 22:07:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4251. 11-Jul-2019 22:07:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:07:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:07:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 11-Jul-2019 22:07:51

Channel:Brightfield set at:11-Jul-2019 22:07:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 22:07:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:07:51

Channel configuration set to: GFP: 11-Jul-2019 22:07:52

Channel:GFP set at:11-Jul-2019 22:07:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 22:07:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:07:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:07:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 22:07:56

Channel:Brightfield set at:11-Jul-2019 22:07:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 22:07:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:07:56

Channel configuration set to: GFP: 11-Jul-2019 22:07:57

Channel:GFP set at:11-Jul-2019 22:07:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 22:07:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:07:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:07:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.3

PFS not locked. Waiting for lock...

PFS took 0.050000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:00

Channel:Brightfield set at:11-Jul-2019 22:08:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 22:08:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:01

Channel configuration set to: GFP: 11-Jul-2019 22:08:02

Channel:GFP set at:11-Jul-2019 22:08:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 22:08:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:06

Channel:Brightfield set at:11-Jul-2019 22:08:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 22:08:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:06

Channel configuration set to: GFP: 11-Jul-2019 22:08:07

Channel:GFP set at:11-Jul-2019 22:08:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 22:08:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:11

Channel:Brightfield set at:11-Jul-2019 22:08:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 22:08:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:12

Channel configuration set to: GFP: 11-Jul-2019 22:08:13

Channel:GFP set at:11-Jul-2019 22:08:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 22:08:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:16

Channel:Brightfield set at:11-Jul-2019 22:08:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 22:08:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:16

Channel configuration set to: GFP: 11-Jul-2019 22:08:18

Channel:GFP set at:11-Jul-2019 22:08:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 22:08:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:21

Channel:Brightfield set at:11-Jul-2019 22:08:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 22:08:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:22

Channel configuration set to: GFP: 11-Jul-2019 22:08:23

Channel:GFP set at:11-Jul-2019 22:08:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 22:08:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:26

Channel:Brightfield set at:11-Jul-2019 22:08:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 22:08:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:27

Channel configuration set to: GFP: 11-Jul-2019 22:08:28

Channel:GFP set at:11-Jul-2019 22:08:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 22:08:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.825

PFS not locked. Waiting for lock...

PFS took 0.700000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:31

Channel:Brightfield set at:11-Jul-2019 22:08:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 22:08:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:32

Channel configuration set to: GFP: 11-Jul-2019 22:08:33

Channel:GFP set at:11-Jul-2019 22:08:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 11-Jul-2019 22:08:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:37

Channel:Brightfield set at:11-Jul-2019 22:08:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 22:08:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:37

Channel configuration set to: GFP: 11-Jul-2019 22:08:38

Channel:GFP set at:11-Jul-2019 22:08:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 22:08:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:43

Channel:Brightfield set at:11-Jul-2019 22:08:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 22:08:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:43

Channel configuration set to: GFP: 11-Jul-2019 22:08:44

Channel:GFP set at:11-Jul-2019 22:08:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 22:08:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:48

Channel:Brightfield set at:11-Jul-2019 22:08:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 22:08:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:48

Channel configuration set to: GFP: 11-Jul-2019 22:08:49

Channel:GFP set at:11-Jul-2019 22:08:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 22:08:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.175

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:53

Channel:Brightfield set at:11-Jul-2019 22:08:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 22:08:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:53

Channel configuration set to: GFP: 11-Jul-2019 22:08:54

Channel:GFP set at:11-Jul-2019 22:08:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 11-Jul-2019 22:08:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-4.95

Channel configuration set to: Brightfield: 11-Jul-2019 22:08:58

Channel:Brightfield set at:11-Jul-2019 22:08:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 11-Jul-2019 22:08:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:08:58

Channel configuration set to: GFP: 11-Jul-2019 22:09:00

Channel:GFP set at:11-Jul-2019 22:09:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 11-Jul-2019 22:09:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:09:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:09:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 22:09:03

Channel:Brightfield set at:11-Jul-2019 22:09:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 22:09:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:09:03

Channel configuration set to: GFP: 11-Jul-2019 22:09:05

Channel:GFP set at:11-Jul-2019 22:09:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 22:09:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:09:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:69 completed at:11-Jul-2019 22:09:07

Time since start of timelapse:20492.8439

PFS is locked

Time to next time point:207.7418



------Time point_70------

Memory available for all arrays: 61093289984

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:12:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.8

Channel configuration set to: Brightfield: 11-Jul-2019 22:12:36

Channel:Brightfield set at:11-Jul-2019 22:12:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 22:12:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:12:36

Channel configuration set to: GFP: 11-Jul-2019 22:12:38

Channel:GFP set at:11-Jul-2019 22:12:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 11-Jul-2019 22:12:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:12:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:12:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.1

Channel configuration set to: Brightfield: 11-Jul-2019 22:12:41

Channel:Brightfield set at:11-Jul-2019 22:12:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 22:12:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:12:42

Channel configuration set to: GFP: 11-Jul-2019 22:12:43

Channel:GFP set at:11-Jul-2019 22:12:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 22:12:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:12:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:12:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.625

Channel configuration set to: Brightfield: 11-Jul-2019 22:12:46

Channel:Brightfield set at:11-Jul-2019 22:12:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 11-Jul-2019 22:12:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:12:47

Channel configuration set to: GFP: 11-Jul-2019 22:12:48

Channel:GFP set at:11-Jul-2019 22:12:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 11-Jul-2019 22:12:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:12:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:12:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.125

Channel configuration set to: Brightfield: 11-Jul-2019 22:12:51

Channel:Brightfield set at:11-Jul-2019 22:12:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 22:12:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:12:52

Channel configuration set to: GFP: 11-Jul-2019 22:12:53

Channel:GFP set at:11-Jul-2019 22:12:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 22:12:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:12:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:12:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.4

Channel configuration set to: Brightfield: 11-Jul-2019 22:12:57

Channel:Brightfield set at:11-Jul-2019 22:12:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 22:12:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:12:57

Channel configuration set to: GFP: 11-Jul-2019 22:12:58

Channel:GFP set at:11-Jul-2019 22:12:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 22:12:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:12:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:02

Channel:Brightfield set at:11-Jul-2019 22:13:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 22:13:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:02

Channel configuration set to: GFP: 11-Jul-2019 22:13:04

Channel:GFP set at:11-Jul-2019 22:13:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 22:13:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:07

Channel:Brightfield set at:11-Jul-2019 22:13:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 22:13:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:07

Channel configuration set to: GFP: 11-Jul-2019 22:13:08

Channel:GFP set at:11-Jul-2019 22:13:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 22:13:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:12

Channel:Brightfield set at:11-Jul-2019 22:13:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 22:13:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:12

Channel configuration set to: GFP: 11-Jul-2019 22:13:13

Channel:GFP set at:11-Jul-2019 22:13:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 22:13:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:17

Channel:Brightfield set at:11-Jul-2019 22:13:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:13:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:17

Channel configuration set to: GFP: 11-Jul-2019 22:13:19

Channel:GFP set at:11-Jul-2019 22:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:13:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.925

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:22

Channel:Brightfield set at:11-Jul-2019 22:13:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 22:13:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:22

Channel configuration set to: GFP: 11-Jul-2019 22:13:23

Channel:GFP set at:11-Jul-2019 22:13:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0001. 11-Jul-2019 22:13:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:27

Channel:Brightfield set at:11-Jul-2019 22:13:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 11-Jul-2019 22:13:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:27

Channel configuration set to: GFP: 11-Jul-2019 22:13:28

Channel:GFP set at:11-Jul-2019 22:13:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 11-Jul-2019 22:13:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:32

Channel:Brightfield set at:11-Jul-2019 22:13:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 11-Jul-2019 22:13:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:32

Channel configuration set to: GFP: 11-Jul-2019 22:13:34

Channel:GFP set at:11-Jul-2019 22:13:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 11-Jul-2019 22:13:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:37

Channel:Brightfield set at:11-Jul-2019 22:13:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 22:13:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:38

Channel configuration set to: GFP: 11-Jul-2019 22:13:39

Channel:GFP set at:11-Jul-2019 22:13:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 22:13:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:42

Channel:Brightfield set at:11-Jul-2019 22:13:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 22:13:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:42

Channel configuration set to: GFP: 11-Jul-2019 22:13:44

Channel:GFP set at:11-Jul-2019 22:13:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 22:13:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.575

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:48

Channel:Brightfield set at:11-Jul-2019 22:13:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 22:13:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:48

Channel configuration set to: GFP: 11-Jul-2019 22:13:49

Channel:GFP set at:11-Jul-2019 22:13:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 22:13:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:52

Channel:Brightfield set at:11-Jul-2019 22:13:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 22:13:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:52

Channel configuration set to: GFP: 11-Jul-2019 22:13:54

Channel:GFP set at:11-Jul-2019 22:13:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 11-Jul-2019 22:13:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 22:13:57

Channel:Brightfield set at:11-Jul-2019 22:13:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 11-Jul-2019 22:13:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:57

Channel configuration set to: GFP: 11-Jul-2019 22:13:59

Channel:GFP set at:11-Jul-2019 22:13:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 11-Jul-2019 22:13:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:13:59

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:14:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 22:14:02

Channel:Brightfield set at:11-Jul-2019 22:14:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 22:14:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:14:03

Channel configuration set to: GFP: 11-Jul-2019 22:14:04

Channel:GFP set at:11-Jul-2019 22:14:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 22:14:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:14:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:70 completed at:11-Jul-2019 22:14:06

Time since start of timelapse:20792.3496

PFS is locked

Time to next time point:208.2376



------Time point_71------

Memory available for all arrays: 61095038976

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:17:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.875

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:17:36

Channel:Brightfield set at:11-Jul-2019 22:17:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3001. 11-Jul-2019 22:17:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:17:37

Channel configuration set to: GFP: 11-Jul-2019 22:17:38

Channel:GFP set at:11-Jul-2019 22:17:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 22:17:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:17:39

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:17:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.225

Channel configuration set to: Brightfield: 11-Jul-2019 22:17:42

Channel:Brightfield set at:11-Jul-2019 22:17:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 22:17:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:17:42

Channel configuration set to: GFP: 11-Jul-2019 22:17:43

Channel:GFP set at:11-Jul-2019 22:17:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 22:17:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:17:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:17:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.725

Channel configuration set to: Brightfield: 11-Jul-2019 22:17:47

Channel:Brightfield set at:11-Jul-2019 22:17:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 11-Jul-2019 22:17:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:17:47

Channel configuration set to: GFP: 11-Jul-2019 22:17:48

Channel:GFP set at:11-Jul-2019 22:17:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 11-Jul-2019 22:17:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:17:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:17:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.125

Channel configuration set to: Brightfield: 11-Jul-2019 22:17:52

Channel:Brightfield set at:11-Jul-2019 22:17:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 22:17:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:17:52

Channel configuration set to: GFP: 11-Jul-2019 22:17:53

Channel:GFP set at:11-Jul-2019 22:17:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 22:17:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:17:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:17:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 11-Jul-2019 22:17:57

Channel:Brightfield set at:11-Jul-2019 22:17:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 22:17:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:17:57

Channel configuration set to: GFP: 11-Jul-2019 22:17:59

Channel:GFP set at:11-Jul-2019 22:17:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 22:17:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:17:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:02

Channel:Brightfield set at:11-Jul-2019 22:18:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 22:18:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:03

Channel configuration set to: GFP: 11-Jul-2019 22:18:04

Channel:GFP set at:11-Jul-2019 22:18:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 22:18:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:07

Channel:Brightfield set at:11-Jul-2019 22:18:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 11-Jul-2019 22:18:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:08

Channel configuration set to: GFP: 11-Jul-2019 22:18:09

Channel:GFP set at:11-Jul-2019 22:18:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 22:18:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:13

Channel:Brightfield set at:11-Jul-2019 22:18:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 22:18:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:13

Channel configuration set to: GFP: 11-Jul-2019 22:18:15

Channel:GFP set at:11-Jul-2019 22:18:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 22:18:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.7

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:18

Channel:Brightfield set at:11-Jul-2019 22:18:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 22:18:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:19

Channel configuration set to: GFP: 11-Jul-2019 22:18:20

Channel:GFP set at:11-Jul-2019 22:18:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 22:18:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:23

Channel:Brightfield set at:11-Jul-2019 22:18:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 22:18:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:23

Channel configuration set to: GFP: 11-Jul-2019 22:18:25

Channel:GFP set at:11-Jul-2019 22:18:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 11-Jul-2019 22:18:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:28

Channel:Brightfield set at:11-Jul-2019 22:18:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 11-Jul-2019 22:18:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:28

Channel configuration set to: GFP: 11-Jul-2019 22:18:29

Channel:GFP set at:11-Jul-2019 22:18:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 11-Jul-2019 22:18:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:33

Channel:Brightfield set at:11-Jul-2019 22:18:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 22:18:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:33

Channel configuration set to: GFP: 11-Jul-2019 22:18:34

Channel:GFP set at:11-Jul-2019 22:18:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 22:18:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:38

Channel:Brightfield set at:11-Jul-2019 22:18:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 22:18:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:39

Channel configuration set to: GFP: 11-Jul-2019 22:18:40

Channel:GFP set at:11-Jul-2019 22:18:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 22:18:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:44

Channel:Brightfield set at:11-Jul-2019 22:18:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 22:18:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:44

Channel configuration set to: GFP: 11-Jul-2019 22:18:45

Channel:GFP set at:11-Jul-2019 22:18:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 22:18:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:49

Channel:Brightfield set at:11-Jul-2019 22:18:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 22:18:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:49

Channel configuration set to: GFP: 11-Jul-2019 22:18:50

Channel:GFP set at:11-Jul-2019 22:18:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 11-Jul-2019 22:18:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:53

Channel:Brightfield set at:11-Jul-2019 22:18:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 11-Jul-2019 22:18:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:53

Channel configuration set to: GFP: 11-Jul-2019 22:18:55

Channel:GFP set at:11-Jul-2019 22:18:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 11-Jul-2019 22:18:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.15

Channel configuration set to: Brightfield: 11-Jul-2019 22:18:58

Channel:Brightfield set at:11-Jul-2019 22:18:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 11-Jul-2019 22:18:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:18:59

Channel configuration set to: GFP: 11-Jul-2019 22:19:00

Channel:GFP set at:11-Jul-2019 22:19:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 11-Jul-2019 22:19:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:19:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:19:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 11-Jul-2019 22:19:03

Channel:Brightfield set at:11-Jul-2019 22:19:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 22:19:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:19:04

Channel configuration set to: GFP: 11-Jul-2019 22:19:05

Channel:GFP set at:11-Jul-2019 22:19:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 11-Jul-2019 22:19:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:19:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:71 completed at:11-Jul-2019 22:19:07

Time since start of timelapse:21093.2984

PFS is locked

Time to next time point:207.2901



------Time point_72------

Memory available for all arrays: 61103128576

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:22:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.875

Channel configuration set to: Brightfield: 11-Jul-2019 22:22:36

Channel:Brightfield set at:11-Jul-2019 22:22:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 22:22:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:22:36

Channel configuration set to: GFP: 11-Jul-2019 22:22:38

Channel:GFP set at:11-Jul-2019 22:22:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 22:22:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:22:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:22:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 11-Jul-2019 22:22:41

Channel:Brightfield set at:11-Jul-2019 22:22:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 22:22:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:22:41

Channel configuration set to: GFP: 11-Jul-2019 22:22:43

Channel:GFP set at:11-Jul-2019 22:22:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 22:22:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:22:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:22:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 11-Jul-2019 22:22:46

Channel:Brightfield set at:11-Jul-2019 22:22:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9001. 11-Jul-2019 22:22:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:22:46

Channel configuration set to: GFP: 11-Jul-2019 22:22:47

Channel:GFP set at:11-Jul-2019 22:22:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 11-Jul-2019 22:22:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:22:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:22:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.9

Channel configuration set to: Brightfield: 11-Jul-2019 22:22:51

Channel:Brightfield set at:11-Jul-2019 22:22:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 22:22:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:22:51

Channel configuration set to: GFP: 11-Jul-2019 22:22:52

Channel:GFP set at:11-Jul-2019 22:22:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 11-Jul-2019 22:22:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:22:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:22:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 11-Jul-2019 22:22:56

Channel:Brightfield set at:11-Jul-2019 22:22:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 22:22:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:22:56

Channel configuration set to: GFP: 11-Jul-2019 22:22:58

Channel:GFP set at:11-Jul-2019 22:22:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 22:22:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:22:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:01

Channel:Brightfield set at:11-Jul-2019 22:23:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 22:23:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:02

Channel configuration set to: GFP: 11-Jul-2019 22:23:03

Channel:GFP set at:11-Jul-2019 22:23:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 22:23:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:06

Channel:Brightfield set at:11-Jul-2019 22:23:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 22:23:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:06

Channel configuration set to: GFP: 11-Jul-2019 22:23:08

Channel:GFP set at:11-Jul-2019 22:23:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 22:23:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:12

Channel:Brightfield set at:11-Jul-2019 22:23:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 22:23:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:12

Channel configuration set to: GFP: 11-Jul-2019 22:23:13

Channel:GFP set at:11-Jul-2019 22:23:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 22:23:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.65

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:17

Channel:Brightfield set at:11-Jul-2019 22:23:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 11-Jul-2019 22:23:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:17

Channel configuration set to: GFP: 11-Jul-2019 22:23:18

Channel:GFP set at:11-Jul-2019 22:23:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 11-Jul-2019 22:23:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:22

Channel:Brightfield set at:11-Jul-2019 22:23:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 22:23:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:22

Channel configuration set to: GFP: 11-Jul-2019 22:23:23

Channel:GFP set at:11-Jul-2019 22:23:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 22:23:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:26

Channel:Brightfield set at:11-Jul-2019 22:23:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 11-Jul-2019 22:23:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:27

Channel configuration set to: GFP: 11-Jul-2019 22:23:28

Channel:GFP set at:11-Jul-2019 22:23:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 22:23:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:32

Channel:Brightfield set at:11-Jul-2019 22:23:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 11-Jul-2019 22:23:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:32

Channel configuration set to: GFP: 11-Jul-2019 22:23:34

Channel:GFP set at:11-Jul-2019 22:23:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 11-Jul-2019 22:23:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:37

Channel:Brightfield set at:11-Jul-2019 22:23:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 22:23:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:38

Channel configuration set to: GFP: 11-Jul-2019 22:23:39

Channel:GFP set at:11-Jul-2019 22:23:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 22:23:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:43

Channel:Brightfield set at:11-Jul-2019 22:23:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7001. 11-Jul-2019 22:23:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:43

Channel configuration set to: GFP: 11-Jul-2019 22:23:45

Channel:GFP set at:11-Jul-2019 22:23:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 22:23:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:48

Channel:Brightfield set at:11-Jul-2019 22:23:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 22:23:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:48

Channel configuration set to: GFP: 11-Jul-2019 22:23:49

Channel:GFP set at:11-Jul-2019 22:23:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 22:23:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:53

Channel:Brightfield set at:11-Jul-2019 22:23:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 22:23:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:53

Channel configuration set to: GFP: 11-Jul-2019 22:23:55

Channel:GFP set at:11-Jul-2019 22:23:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 11-Jul-2019 22:23:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 11-Jul-2019 22:23:58

Channel:Brightfield set at:11-Jul-2019 22:23:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 22:23:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:23:58

Channel configuration set to: GFP: 11-Jul-2019 22:23:59

Channel:GFP set at:11-Jul-2019 22:23:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 11-Jul-2019 22:24:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:24:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:24:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-4.75

Channel configuration set to: Brightfield: 11-Jul-2019 22:24:02

Channel:Brightfield set at:11-Jul-2019 22:24:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 22:24:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:24:02

Channel configuration set to: GFP: 11-Jul-2019 22:24:04

Channel:GFP set at:11-Jul-2019 22:24:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 22:24:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:24:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:72 completed at:11-Jul-2019 22:24:06

Time since start of timelapse:21392.4577

PFS is locked

Time to next time point:208.1324



------Time point_73------

Memory available for all arrays: 61099175936

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:27:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.875

Channel configuration set to: Brightfield: 11-Jul-2019 22:27:36

Channel:Brightfield set at:11-Jul-2019 22:27:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 22:27:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:27:36

Channel configuration set to: GFP: 11-Jul-2019 22:27:38

Channel:GFP set at:11-Jul-2019 22:27:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 22:27:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:27:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:27:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.425

Channel configuration set to: Brightfield: 11-Jul-2019 22:27:41

Channel:Brightfield set at:11-Jul-2019 22:27:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 11-Jul-2019 22:27:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:27:41

Channel configuration set to: GFP: 11-Jul-2019 22:27:42

Channel:GFP set at:11-Jul-2019 22:27:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7751. 11-Jul-2019 22:27:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:27:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:27:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.65

Channel configuration set to: Brightfield: 11-Jul-2019 22:27:46

Channel:Brightfield set at:11-Jul-2019 22:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 22:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:27:46

Channel configuration set to: GFP: 11-Jul-2019 22:27:48

Channel:GFP set at:11-Jul-2019 22:27:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 11-Jul-2019 22:27:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:27:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:27:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.075

Channel configuration set to: Brightfield: 11-Jul-2019 22:27:51

Channel:Brightfield set at:11-Jul-2019 22:27:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 11-Jul-2019 22:27:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:27:51

Channel configuration set to: GFP: 11-Jul-2019 22:27:53

Channel:GFP set at:11-Jul-2019 22:27:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 11-Jul-2019 22:27:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:27:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:27:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 22:27:56

Channel:Brightfield set at:11-Jul-2019 22:27:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 22:27:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:27:57

Channel configuration set to: GFP: 11-Jul-2019 22:27:58

Channel:GFP set at:11-Jul-2019 22:27:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 22:27:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:27:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:02

Channel:Brightfield set at:11-Jul-2019 22:28:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 22:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:02

Channel configuration set to: GFP: 11-Jul-2019 22:28:03

Channel:GFP set at:11-Jul-2019 22:28:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 22:28:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:07

Channel:Brightfield set at:11-Jul-2019 22:28:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 22:28:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:07

Channel configuration set to: GFP: 11-Jul-2019 22:28:08

Channel:GFP set at:11-Jul-2019 22:28:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 22:28:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:12

Channel:Brightfield set at:11-Jul-2019 22:28:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 22:28:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:12

Channel configuration set to: GFP: 11-Jul-2019 22:28:13

Channel:GFP set at:11-Jul-2019 22:28:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 22:28:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:17

Channel:Brightfield set at:11-Jul-2019 22:28:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 11-Jul-2019 22:28:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:17

Channel configuration set to: GFP: 11-Jul-2019 22:28:19

Channel:GFP set at:11-Jul-2019 22:28:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:28:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:22

Channel:Brightfield set at:11-Jul-2019 22:28:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 22:28:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:22

Channel configuration set to: GFP: 11-Jul-2019 22:28:23

Channel:GFP set at:11-Jul-2019 22:28:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 22:28:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:27

Channel:Brightfield set at:11-Jul-2019 22:28:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 11-Jul-2019 22:28:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:27

Channel configuration set to: GFP: 11-Jul-2019 22:28:28

Channel:GFP set at:11-Jul-2019 22:28:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 11-Jul-2019 22:28:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:32

Channel:Brightfield set at:11-Jul-2019 22:28:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 11-Jul-2019 22:28:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:32

Channel configuration set to: GFP: 11-Jul-2019 22:28:34

Channel:GFP set at:11-Jul-2019 22:28:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5001. 11-Jul-2019 22:28:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:37

Channel:Brightfield set at:11-Jul-2019 22:28:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 22:28:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:37

Channel configuration set to: GFP: 11-Jul-2019 22:28:39

Channel:GFP set at:11-Jul-2019 22:28:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 22:28:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:41

Channel:Brightfield set at:11-Jul-2019 22:28:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 22:28:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:42

Channel configuration set to: GFP: 11-Jul-2019 22:28:43

Channel:GFP set at:11-Jul-2019 22:28:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 22:28:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.6

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:47

Channel:Brightfield set at:11-Jul-2019 22:28:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 22:28:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:47

Channel configuration set to: GFP: 11-Jul-2019 22:28:48

Channel:GFP set at:11-Jul-2019 22:28:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 22:28:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:52

Channel:Brightfield set at:11-Jul-2019 22:28:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 22:28:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:52

Channel configuration set to: GFP: 11-Jul-2019 22:28:53

Channel:GFP set at:11-Jul-2019 22:28:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 22:28:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 11-Jul-2019 22:28:56

Channel:Brightfield set at:11-Jul-2019 22:28:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 22:28:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:57

Channel configuration set to: GFP: 11-Jul-2019 22:28:58

Channel:GFP set at:11-Jul-2019 22:28:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 11-Jul-2019 22:28:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:28:58

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:29:00

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 22:29:01

Channel:Brightfield set at:11-Jul-2019 22:29:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 22:29:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:29:02

Channel configuration set to: GFP: 11-Jul-2019 22:29:03

Channel:GFP set at:11-Jul-2019 22:29:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 22:29:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:29:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:73 completed at:11-Jul-2019 22:29:05

Time since start of timelapse:21691.4919

PFS is locked

Time to next time point:209.0998



------Time point_74------

Memory available for all arrays: 61099585536

Memory used by Matlab: 2209120256

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:32:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 11-Jul-2019 22:32:36

Channel:Brightfield set at:11-Jul-2019 22:32:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 22:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:32:37

Channel configuration set to: GFP: 11-Jul-2019 22:32:38

Channel:GFP set at:11-Jul-2019 22:32:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 11-Jul-2019 22:32:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:32:39

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:32:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.5

Channel configuration set to: Brightfield: 11-Jul-2019 22:32:41

Channel:Brightfield set at:11-Jul-2019 22:32:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5501. 11-Jul-2019 22:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:32:41

Channel configuration set to: GFP: 11-Jul-2019 22:32:43

Channel:GFP set at:11-Jul-2019 22:32:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5501. 11-Jul-2019 22:32:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:32:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:32:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.95

Channel configuration set to: Brightfield: 11-Jul-2019 22:32:46

Channel:Brightfield set at:11-Jul-2019 22:32:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 22:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:32:47

Channel configuration set to: GFP: 11-Jul-2019 22:32:48

Channel:GFP set at:11-Jul-2019 22:32:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 22:32:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:32:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:32:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 11-Jul-2019 22:32:51

Channel:Brightfield set at:11-Jul-2019 22:32:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 22:32:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:32:52

Channel configuration set to: GFP: 11-Jul-2019 22:32:53

Channel:GFP set at:11-Jul-2019 22:32:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 22:32:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:32:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:32:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.6

Channel configuration set to: Brightfield: 11-Jul-2019 22:32:57

Channel:Brightfield set at:11-Jul-2019 22:32:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 22:32:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:32:57

Channel configuration set to: GFP: 11-Jul-2019 22:32:58

Channel:GFP set at:11-Jul-2019 22:32:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 22:32:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:32:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:02

Channel:Brightfield set at:11-Jul-2019 22:33:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 22:33:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:02

Channel configuration set to: GFP: 11-Jul-2019 22:33:03

Channel:GFP set at:11-Jul-2019 22:33:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:33:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:07

Channel:Brightfield set at:11-Jul-2019 22:33:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 22:33:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:07

Channel configuration set to: GFP: 11-Jul-2019 22:33:08

Channel:GFP set at:11-Jul-2019 22:33:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 22:33:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:12

Channel:Brightfield set at:11-Jul-2019 22:33:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 22:33:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:13

Channel configuration set to: GFP: 11-Jul-2019 22:33:14

Channel:GFP set at:11-Jul-2019 22:33:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 22:33:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:18

Channel:Brightfield set at:11-Jul-2019 22:33:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 22:33:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:18

Channel configuration set to: GFP: 11-Jul-2019 22:33:19

Channel:GFP set at:11-Jul-2019 22:33:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 22:33:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:23

Channel:Brightfield set at:11-Jul-2019 22:33:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 11-Jul-2019 22:33:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:23

Channel configuration set to: GFP: 11-Jul-2019 22:33:25

Channel:GFP set at:11-Jul-2019 22:33:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 22:33:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:28

Channel:Brightfield set at:11-Jul-2019 22:33:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 22:33:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:29

Channel configuration set to: GFP: 11-Jul-2019 22:33:30

Channel:GFP set at:11-Jul-2019 22:33:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 22:33:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:34

Channel:Brightfield set at:11-Jul-2019 22:33:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 11-Jul-2019 22:33:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:34

Channel configuration set to: GFP: 11-Jul-2019 22:33:35

Channel:GFP set at:11-Jul-2019 22:33:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 22:33:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:39

Channel:Brightfield set at:11-Jul-2019 22:33:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 22:33:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:39

Channel configuration set to: GFP: 11-Jul-2019 22:33:41

Channel:GFP set at:11-Jul-2019 22:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 11-Jul-2019 22:33:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:44

Channel:Brightfield set at:11-Jul-2019 22:33:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 22:33:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:44

Channel configuration set to: GFP: 11-Jul-2019 22:33:46

Channel:GFP set at:11-Jul-2019 22:33:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 22:33:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:49

Channel:Brightfield set at:11-Jul-2019 22:33:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 22:33:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:49

Channel configuration set to: GFP: 11-Jul-2019 22:33:51

Channel:GFP set at:11-Jul-2019 22:33:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 22:33:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:55

Channel:Brightfield set at:11-Jul-2019 22:33:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7251. 11-Jul-2019 22:33:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:55

Channel configuration set to: GFP: 11-Jul-2019 22:33:56

Channel:GFP set at:11-Jul-2019 22:33:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 22:33:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:33:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.175

PFS not locked. Waiting for lock...

PFS took 0.400000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:33:59

Channel:Brightfield set at:11-Jul-2019 22:33:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 11-Jul-2019 22:34:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:34:00

Channel configuration set to: GFP: 11-Jul-2019 22:34:01

Channel:GFP set at:11-Jul-2019 22:34:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 11-Jul-2019 22:34:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:34:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:34:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 22:34:05

Channel:Brightfield set at:11-Jul-2019 22:34:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 11-Jul-2019 22:34:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:34:05

Channel configuration set to: GFP: 11-Jul-2019 22:34:06

Channel:GFP set at:11-Jul-2019 22:34:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 22:34:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:34:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:74 completed at:11-Jul-2019 22:34:08

Time since start of timelapse:21994.5852

PFS is locked

Time to next time point:206.0079



------Time point_75------

Memory available for all arrays: 61100244992

Memory used by Matlab: 2209218560

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:37:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 11-Jul-2019 22:37:36

Channel:Brightfield set at:11-Jul-2019 22:37:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 22:37:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:37:36

Channel configuration set to: GFP: 11-Jul-2019 22:37:38

Channel:GFP set at:11-Jul-2019 22:37:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 22:37:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:37:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:37:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.875

Channel configuration set to: Brightfield: 11-Jul-2019 22:37:41

Channel:Brightfield set at:11-Jul-2019 22:37:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 11-Jul-2019 22:37:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:37:41

Channel configuration set to: GFP: 11-Jul-2019 22:37:42

Channel:GFP set at:11-Jul-2019 22:37:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 11-Jul-2019 22:37:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:37:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:37:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.05

Channel configuration set to: Brightfield: 11-Jul-2019 22:37:46

Channel:Brightfield set at:11-Jul-2019 22:37:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 22:37:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:37:46

Channel configuration set to: GFP: 11-Jul-2019 22:37:47

Channel:GFP set at:11-Jul-2019 22:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 22:37:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:37:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:37:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.575

Channel configuration set to: Brightfield: 11-Jul-2019 22:37:51

Channel:Brightfield set at:11-Jul-2019 22:37:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 11-Jul-2019 22:37:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:37:51

Channel configuration set to: GFP: 11-Jul-2019 22:37:52

Channel:GFP set at:11-Jul-2019 22:37:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 22:37:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:37:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:37:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.725

Channel configuration set to: Brightfield: 11-Jul-2019 22:37:56

Channel:Brightfield set at:11-Jul-2019 22:37:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 11-Jul-2019 22:37:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:37:56

Channel configuration set to: GFP: 11-Jul-2019 22:37:58

Channel:GFP set at:11-Jul-2019 22:37:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 11-Jul-2019 22:37:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:37:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:01

Channel:Brightfield set at:11-Jul-2019 22:38:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:38:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:02

Channel configuration set to: GFP: 11-Jul-2019 22:38:03

Channel:GFP set at:11-Jul-2019 22:38:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:38:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.55

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:06

Channel:Brightfield set at:11-Jul-2019 22:38:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 22:38:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:07

Channel configuration set to: GFP: 11-Jul-2019 22:38:08

Channel:GFP set at:11-Jul-2019 22:38:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 22:38:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:12

Channel:Brightfield set at:11-Jul-2019 22:38:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 22:38:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:12

Channel configuration set to: GFP: 11-Jul-2019 22:38:13

Channel:GFP set at:11-Jul-2019 22:38:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 22:38:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:17

Channel:Brightfield set at:11-Jul-2019 22:38:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 22:38:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:17

Channel configuration set to: GFP: 11-Jul-2019 22:38:19

Channel:GFP set at:11-Jul-2019 22:38:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 22:38:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:21

Channel:Brightfield set at:11-Jul-2019 22:38:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 22:38:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:22

Channel configuration set to: GFP: 11-Jul-2019 22:38:23

Channel:GFP set at:11-Jul-2019 22:38:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 22:38:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:26

Channel:Brightfield set at:11-Jul-2019 22:38:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 22:38:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:26

Channel configuration set to: GFP: 11-Jul-2019 22:38:28

Channel:GFP set at:11-Jul-2019 22:38:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 22:38:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:31

Channel:Brightfield set at:11-Jul-2019 22:38:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8251. 11-Jul-2019 22:38:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:32

Channel configuration set to: GFP: 11-Jul-2019 22:38:33

Channel:GFP set at:11-Jul-2019 22:38:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8251. 11-Jul-2019 22:38:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:37

Channel:Brightfield set at:11-Jul-2019 22:38:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 11-Jul-2019 22:38:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:37

Channel configuration set to: GFP: 11-Jul-2019 22:38:38

Channel:GFP set at:11-Jul-2019 22:38:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 11-Jul-2019 22:38:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:42

Channel:Brightfield set at:11-Jul-2019 22:38:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 22:38:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:42

Channel configuration set to: GFP: 11-Jul-2019 22:38:44

Channel:GFP set at:11-Jul-2019 22:38:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 22:38:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.95

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:47

Channel:Brightfield set at:11-Jul-2019 22:38:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 22:38:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:47

Channel configuration set to: GFP: 11-Jul-2019 22:38:48

Channel:GFP set at:11-Jul-2019 22:38:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 11-Jul-2019 22:38:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:51

Channel:Brightfield set at:11-Jul-2019 22:38:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 22:38:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:51

Channel configuration set to: GFP: 11-Jul-2019 22:38:53

Channel:GFP set at:11-Jul-2019 22:38:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 22:38:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:53

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 11-Jul-2019 22:38:56

Channel:Brightfield set at:11-Jul-2019 22:38:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 11-Jul-2019 22:38:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:57

Channel configuration set to: GFP: 11-Jul-2019 22:38:58

Channel:GFP set at:11-Jul-2019 22:38:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 22:38:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:38:58

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:39:00

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 22:39:02

Channel:Brightfield set at:11-Jul-2019 22:39:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 22:39:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:39:02

Channel configuration set to: GFP: 11-Jul-2019 22:39:03

Channel:GFP set at:11-Jul-2019 22:39:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 11-Jul-2019 22:39:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:39:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:75 completed at:11-Jul-2019 22:39:05

Time since start of timelapse:22291.6403

PFS is locked

Time to next time point:208.9544



------Time point_76------

Memory available for all arrays: 61098999808

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:42:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.025

Channel configuration set to: Brightfield: 11-Jul-2019 22:42:36

Channel:Brightfield set at:11-Jul-2019 22:42:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 22:42:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:42:36

Channel configuration set to: GFP: 11-Jul-2019 22:42:38

Channel:GFP set at:11-Jul-2019 22:42:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 22:42:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:42:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:42:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.6

PFS not locked. Waiting for lock...

PFS took 0.450000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:42:41

Channel:Brightfield set at:11-Jul-2019 22:42:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 22:42:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:42:41

Channel configuration set to: GFP: 11-Jul-2019 22:42:43

Channel:GFP set at:11-Jul-2019 22:42:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 22:42:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:42:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:42:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.325

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:42:47

Channel:Brightfield set at:11-Jul-2019 22:42:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0501. 11-Jul-2019 22:42:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:42:47

Channel configuration set to: GFP: 11-Jul-2019 22:42:48

Channel:GFP set at:11-Jul-2019 22:42:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0501. 11-Jul-2019 22:42:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:42:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:42:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 11-Jul-2019 22:42:52

Channel:Brightfield set at:11-Jul-2019 22:42:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 11-Jul-2019 22:42:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:42:52

Channel configuration set to: GFP: 11-Jul-2019 22:42:53

Channel:GFP set at:11-Jul-2019 22:42:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 22:42:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:42:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:42:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.5

Channel configuration set to: Brightfield: 11-Jul-2019 22:42:56

Channel:Brightfield set at:11-Jul-2019 22:42:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 22:42:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:42:57

Channel configuration set to: GFP: 11-Jul-2019 22:42:58

Channel:GFP set at:11-Jul-2019 22:42:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 22:42:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:42:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.675

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:01

Channel:Brightfield set at:11-Jul-2019 22:43:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 22:43:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:01

Channel configuration set to: GFP: 11-Jul-2019 22:43:03

Channel:GFP set at:11-Jul-2019 22:43:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 11-Jul-2019 22:43:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:07

Channel:Brightfield set at:11-Jul-2019 22:43:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 22:43:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:07

Channel configuration set to: GFP: 11-Jul-2019 22:43:08

Channel:GFP set at:11-Jul-2019 22:43:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 11-Jul-2019 22:43:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:13

Channel:Brightfield set at:11-Jul-2019 22:43:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 22:43:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:13

Channel configuration set to: GFP: 11-Jul-2019 22:43:14

Channel:GFP set at:11-Jul-2019 22:43:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 22:43:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:18

Channel:Brightfield set at:11-Jul-2019 22:43:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 22:43:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:18

Channel configuration set to: GFP: 11-Jul-2019 22:43:20

Channel:GFP set at:11-Jul-2019 22:43:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 22:43:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:22

Channel:Brightfield set at:11-Jul-2019 22:43:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5501. 11-Jul-2019 22:43:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:23

Channel configuration set to: GFP: 11-Jul-2019 22:43:24

Channel:GFP set at:11-Jul-2019 22:43:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 11-Jul-2019 22:43:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:28

Channel:Brightfield set at:11-Jul-2019 22:43:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 22:43:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:28

Channel configuration set to: GFP: 11-Jul-2019 22:43:29

Channel:GFP set at:11-Jul-2019 22:43:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 22:43:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:33

Channel:Brightfield set at:11-Jul-2019 22:43:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 11-Jul-2019 22:43:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:33

Channel configuration set to: GFP: 11-Jul-2019 22:43:35

Channel:GFP set at:11-Jul-2019 22:43:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 11-Jul-2019 22:43:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.075

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:38

Channel:Brightfield set at:11-Jul-2019 22:43:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 22:43:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:39

Channel configuration set to: GFP: 11-Jul-2019 22:43:40

Channel:GFP set at:11-Jul-2019 22:43:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 22:43:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:43

Channel:Brightfield set at:11-Jul-2019 22:43:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 22:43:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:44

Channel configuration set to: GFP: 11-Jul-2019 22:43:45

Channel:GFP set at:11-Jul-2019 22:43:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 22:43:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:48

Channel:Brightfield set at:11-Jul-2019 22:43:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 22:43:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:48

Channel configuration set to: GFP: 11-Jul-2019 22:43:50

Channel:GFP set at:11-Jul-2019 22:43:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 11-Jul-2019 22:43:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:54

Channel:Brightfield set at:11-Jul-2019 22:43:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 22:43:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:54

Channel configuration set to: GFP: 11-Jul-2019 22:43:56

Channel:GFP set at:11-Jul-2019 22:43:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 11-Jul-2019 22:43:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 11-Jul-2019 22:43:59

Channel:Brightfield set at:11-Jul-2019 22:43:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5001. 11-Jul-2019 22:43:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:43:59

Channel configuration set to: GFP: 11-Jul-2019 22:44:00

Channel:GFP set at:11-Jul-2019 22:44:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5001. 11-Jul-2019 22:44:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:44:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:44:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 22:44:04

Channel:Brightfield set at:11-Jul-2019 22:44:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 22:44:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:44:04

Channel configuration set to: GFP: 11-Jul-2019 22:44:06

Channel:GFP set at:11-Jul-2019 22:44:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 11-Jul-2019 22:44:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:44:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:76 completed at:11-Jul-2019 22:44:08

Time since start of timelapse:22594.1316

PFS is locked

Time to next time point:206.4648



------Time point_77------

Memory available for all arrays: 61095104512

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:47:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 11-Jul-2019 22:47:36

Channel:Brightfield set at:11-Jul-2019 22:47:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 22:47:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:47:36

Channel configuration set to: GFP: 11-Jul-2019 22:47:38

Channel:GFP set at:11-Jul-2019 22:47:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 22:47:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:47:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:47:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.725

Channel configuration set to: Brightfield: 11-Jul-2019 22:47:41

Channel:Brightfield set at:11-Jul-2019 22:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 22:47:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:47:41

Channel configuration set to: GFP: 11-Jul-2019 22:47:42

Channel:GFP set at:11-Jul-2019 22:47:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 11-Jul-2019 22:47:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:47:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:47:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 22:47:45

Channel:Brightfield set at:11-Jul-2019 22:47:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 11-Jul-2019 22:47:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:47:46

Channel configuration set to: GFP: 11-Jul-2019 22:47:47

Channel:GFP set at:11-Jul-2019 22:47:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 11-Jul-2019 22:47:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:47:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:47:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.375

Channel configuration set to: Brightfield: 11-Jul-2019 22:47:50

Channel:Brightfield set at:11-Jul-2019 22:47:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 22:47:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:47:51

Channel configuration set to: GFP: 11-Jul-2019 22:47:52

Channel:GFP set at:11-Jul-2019 22:47:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 22:47:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:47:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:47:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 11-Jul-2019 22:47:56

Channel:Brightfield set at:11-Jul-2019 22:47:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:47:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:47:56

Channel configuration set to: GFP: 11-Jul-2019 22:47:57

Channel:GFP set at:11-Jul-2019 22:47:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:47:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:47:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:01

Channel:Brightfield set at:11-Jul-2019 22:48:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:48:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:01

Channel configuration set to: GFP: 11-Jul-2019 22:48:02

Channel:GFP set at:11-Jul-2019 22:48:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:48:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:06

Channel:Brightfield set at:11-Jul-2019 22:48:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 22:48:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:06

Channel configuration set to: GFP: 11-Jul-2019 22:48:07

Channel:GFP set at:11-Jul-2019 22:48:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 11-Jul-2019 22:48:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:11

Channel:Brightfield set at:11-Jul-2019 22:48:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:48:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:11

Channel configuration set to: GFP: 11-Jul-2019 22:48:12

Channel:GFP set at:11-Jul-2019 22:48:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:48:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:14

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:16

Channel:Brightfield set at:11-Jul-2019 22:48:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 22:48:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:16

Channel configuration set to: GFP: 11-Jul-2019 22:48:17

Channel:GFP set at:11-Jul-2019 22:48:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 11-Jul-2019 22:48:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:21

Channel:Brightfield set at:11-Jul-2019 22:48:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 22:48:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:21

Channel configuration set to: GFP: 11-Jul-2019 22:48:23

Channel:GFP set at:11-Jul-2019 22:48:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 11-Jul-2019 22:48:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:26

Channel:Brightfield set at:11-Jul-2019 22:48:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 22:48:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:27

Channel configuration set to: GFP: 11-Jul-2019 22:48:28

Channel:GFP set at:11-Jul-2019 22:48:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 22:48:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:30

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:32

Channel:Brightfield set at:11-Jul-2019 22:48:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 22:48:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:32

Channel configuration set to: GFP: 11-Jul-2019 22:48:33

Channel:GFP set at:11-Jul-2019 22:48:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 22:48:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:37

Channel:Brightfield set at:11-Jul-2019 22:48:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 11-Jul-2019 22:48:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:38

Channel configuration set to: GFP: 11-Jul-2019 22:48:39

Channel:GFP set at:11-Jul-2019 22:48:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 11-Jul-2019 22:48:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:43

Channel:Brightfield set at:11-Jul-2019 22:48:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 11-Jul-2019 22:48:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:43

Channel configuration set to: GFP: 11-Jul-2019 22:48:44

Channel:GFP set at:11-Jul-2019 22:48:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 11-Jul-2019 22:48:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:47

Channel:Brightfield set at:11-Jul-2019 22:48:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 22:48:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:47

Channel configuration set to: GFP: 11-Jul-2019 22:48:49

Channel:GFP set at:11-Jul-2019 22:48:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 22:48:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:52

Channel:Brightfield set at:11-Jul-2019 22:48:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 22:48:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:53

Channel configuration set to: GFP: 11-Jul-2019 22:48:54

Channel:GFP set at:11-Jul-2019 22:48:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 22:48:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 11-Jul-2019 22:48:57

Channel:Brightfield set at:11-Jul-2019 22:48:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 22:48:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:57

Channel configuration set to: GFP: 11-Jul-2019 22:48:58

Channel:GFP set at:11-Jul-2019 22:48:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 22:48:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:48:59

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:49:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 22:49:02

Channel:Brightfield set at:11-Jul-2019 22:49:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 11-Jul-2019 22:49:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:49:02

Channel configuration set to: GFP: 11-Jul-2019 22:49:04

Channel:GFP set at:11-Jul-2019 22:49:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 22:49:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:49:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:77 completed at:11-Jul-2019 22:49:06

Time since start of timelapse:22892.0573

PFS is locked

Time to next time point:208.5404



------Time point_78------

Memory available for all arrays: 61098991616

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:52:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 11-Jul-2019 22:52:36

Channel:Brightfield set at:11-Jul-2019 22:52:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 22:52:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:52:36

Channel configuration set to: GFP: 11-Jul-2019 22:52:38

Channel:GFP set at:11-Jul-2019 22:52:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 22:52:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:52:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:52:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.45

Channel configuration set to: Brightfield: 11-Jul-2019 22:52:41

Channel:Brightfield set at:11-Jul-2019 22:52:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 11-Jul-2019 22:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:52:41

Channel configuration set to: GFP: 11-Jul-2019 22:52:43

Channel:GFP set at:11-Jul-2019 22:52:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4251. 11-Jul-2019 22:52:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:52:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:52:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 22:52:46

Channel:Brightfield set at:11-Jul-2019 22:52:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 11-Jul-2019 22:52:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:52:46

Channel configuration set to: GFP: 11-Jul-2019 22:52:47

Channel:GFP set at:11-Jul-2019 22:52:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 11-Jul-2019 22:52:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:52:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:52:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.525

Channel configuration set to: Brightfield: 11-Jul-2019 22:52:51

Channel:Brightfield set at:11-Jul-2019 22:52:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 22:52:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:52:51

Channel configuration set to: GFP: 11-Jul-2019 22:52:53

Channel:GFP set at:11-Jul-2019 22:52:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 22:52:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:52:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:52:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 11-Jul-2019 22:52:56

Channel:Brightfield set at:11-Jul-2019 22:52:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 11-Jul-2019 22:52:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:52:56

Channel configuration set to: GFP: 11-Jul-2019 22:52:57

Channel:GFP set at:11-Jul-2019 22:52:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 22:52:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:52:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:52:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.75

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:00

Channel:Brightfield set at:11-Jul-2019 22:53:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 22:53:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:00

Channel configuration set to: GFP: 11-Jul-2019 22:53:02

Channel:GFP set at:11-Jul-2019 22:53:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 22:53:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:06

Channel:Brightfield set at:11-Jul-2019 22:53:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 11-Jul-2019 22:53:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:06

Channel configuration set to: GFP: 11-Jul-2019 22:53:07

Channel:GFP set at:11-Jul-2019 22:53:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 11-Jul-2019 22:53:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.5

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:11

Channel:Brightfield set at:11-Jul-2019 22:53:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 22:53:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:12

Channel configuration set to: GFP: 11-Jul-2019 22:53:13

Channel:GFP set at:11-Jul-2019 22:53:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4501. 11-Jul-2019 22:53:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:17

Channel:Brightfield set at:11-Jul-2019 22:53:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 22:53:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:17

Channel configuration set to: GFP: 11-Jul-2019 22:53:18

Channel:GFP set at:11-Jul-2019 22:53:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 22:53:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:22

Channel:Brightfield set at:11-Jul-2019 22:53:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 11-Jul-2019 22:53:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:22

Channel configuration set to: GFP: 11-Jul-2019 22:53:23

Channel:GFP set at:11-Jul-2019 22:53:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 11-Jul-2019 22:53:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:27

Channel:Brightfield set at:11-Jul-2019 22:53:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 22:53:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:27

Channel configuration set to: GFP: 11-Jul-2019 22:53:28

Channel:GFP set at:11-Jul-2019 22:53:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 11-Jul-2019 22:53:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:32

Channel:Brightfield set at:11-Jul-2019 22:53:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8001. 11-Jul-2019 22:53:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:32

Channel configuration set to: GFP: 11-Jul-2019 22:53:33

Channel:GFP set at:11-Jul-2019 22:53:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 11-Jul-2019 22:53:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:37

Channel:Brightfield set at:11-Jul-2019 22:53:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 11-Jul-2019 22:53:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:38

Channel configuration set to: GFP: 11-Jul-2019 22:53:39

Channel:GFP set at:11-Jul-2019 22:53:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 22:53:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.6

PFS not locked. Waiting for lock...

PFS took 0.450000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:42

Channel:Brightfield set at:11-Jul-2019 22:53:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 22:53:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:43

Channel configuration set to: GFP: 11-Jul-2019 22:53:44

Channel:GFP set at:11-Jul-2019 22:53:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 22:53:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:47

Channel:Brightfield set at:11-Jul-2019 22:53:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 22:53:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:47

Channel configuration set to: GFP: 11-Jul-2019 22:53:49

Channel:GFP set at:11-Jul-2019 22:53:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 22:53:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:52

Channel:Brightfield set at:11-Jul-2019 22:53:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 22:53:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:53

Channel configuration set to: GFP: 11-Jul-2019 22:53:54

Channel:GFP set at:11-Jul-2019 22:53:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 22:53:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.125

PFS not locked. Waiting for lock...

PFS took 0.400000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:53:57

Channel:Brightfield set at:11-Jul-2019 22:53:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 22:53:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:57

Channel configuration set to: GFP: 11-Jul-2019 22:53:59

Channel:GFP set at:11-Jul-2019 22:53:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 22:53:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:53:59

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:54:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 22:54:02

Channel:Brightfield set at:11-Jul-2019 22:54:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 22:54:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:54:03

Channel configuration set to: GFP: 11-Jul-2019 22:54:04

Channel:GFP set at:11-Jul-2019 22:54:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 22:54:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:54:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:78 completed at:11-Jul-2019 22:54:06

Time since start of timelapse:23192.3628

PFS is locked

Time to next time point:208.2363



------Time point_79------

Memory available for all arrays: 61097926656

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:57:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 11-Jul-2019 22:57:36

Channel:Brightfield set at:11-Jul-2019 22:57:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 22:57:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:57:36

Channel configuration set to: GFP: 11-Jul-2019 22:57:38

Channel:GFP set at:11-Jul-2019 22:57:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 22:57:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:57:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:57:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 11-Jul-2019 22:57:41

Channel:Brightfield set at:11-Jul-2019 22:57:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 22:57:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:57:41

Channel configuration set to: GFP: 11-Jul-2019 22:57:43

Channel:GFP set at:11-Jul-2019 22:57:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 22:57:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:57:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:57:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.95

Channel configuration set to: Brightfield: 11-Jul-2019 22:57:46

Channel:Brightfield set at:11-Jul-2019 22:57:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 11-Jul-2019 22:57:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:57:47

Channel configuration set to: GFP: 11-Jul-2019 22:57:48

Channel:GFP set at:11-Jul-2019 22:57:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 11-Jul-2019 22:57:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:57:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:57:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.55

Channel configuration set to: Brightfield: 11-Jul-2019 22:57:51

Channel:Brightfield set at:11-Jul-2019 22:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 22:57:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:57:52

Channel configuration set to: GFP: 11-Jul-2019 22:57:53

Channel:GFP set at:11-Jul-2019 22:57:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 22:57:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:57:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:57:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 11-Jul-2019 22:57:56

Channel:Brightfield set at:11-Jul-2019 22:57:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:57:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:57:57

Channel configuration set to: GFP: 11-Jul-2019 22:57:58

Channel:GFP set at:11-Jul-2019 22:57:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 22:57:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:57:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:02

Channel:Brightfield set at:11-Jul-2019 22:58:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 22:58:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:02

Channel configuration set to: GFP: 11-Jul-2019 22:58:03

Channel:GFP set at:11-Jul-2019 22:58:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 22:58:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:07

Channel:Brightfield set at:11-Jul-2019 22:58:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 11-Jul-2019 22:58:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:07

Channel configuration set to: GFP: 11-Jul-2019 22:58:08

Channel:GFP set at:11-Jul-2019 22:58:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 11-Jul-2019 22:58:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.475

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:12

Channel:Brightfield set at:11-Jul-2019 22:58:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 22:58:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:13

Channel configuration set to: GFP: 11-Jul-2019 22:58:14

Channel:GFP set at:11-Jul-2019 22:58:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 22:58:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:17

Channel:Brightfield set at:11-Jul-2019 22:58:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 22:58:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:17

Channel configuration set to: GFP: 11-Jul-2019 22:58:19

Channel:GFP set at:11-Jul-2019 22:58:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 11-Jul-2019 22:58:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:22

Channel:Brightfield set at:11-Jul-2019 22:58:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 22:58:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:22

Channel configuration set to: GFP: 11-Jul-2019 22:58:24

Channel:GFP set at:11-Jul-2019 22:58:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 22:58:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:27

Channel:Brightfield set at:11-Jul-2019 22:58:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 22:58:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:27

Channel configuration set to: GFP: 11-Jul-2019 22:58:28

Channel:GFP set at:11-Jul-2019 22:58:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 22:58:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:32

Channel:Brightfield set at:11-Jul-2019 22:58:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 11-Jul-2019 22:58:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:33

Channel configuration set to: GFP: 11-Jul-2019 22:58:34

Channel:GFP set at:11-Jul-2019 22:58:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 22:58:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:37

Channel:Brightfield set at:11-Jul-2019 22:58:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 22:58:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:38

Channel configuration set to: GFP: 11-Jul-2019 22:58:39

Channel:GFP set at:11-Jul-2019 22:58:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 22:58:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.625

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:43

Channel:Brightfield set at:11-Jul-2019 22:58:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 11-Jul-2019 22:58:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:43

Channel configuration set to: GFP: 11-Jul-2019 22:58:44

Channel:GFP set at:11-Jul-2019 22:58:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 22:58:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:48

Channel:Brightfield set at:11-Jul-2019 22:58:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 22:58:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:48

Channel configuration set to: GFP: 11-Jul-2019 22:58:49

Channel:GFP set at:11-Jul-2019 22:58:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 11-Jul-2019 22:58:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:53

Channel:Brightfield set at:11-Jul-2019 22:58:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 22:58:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:53

Channel configuration set to: GFP: 11-Jul-2019 22:58:55

Channel:GFP set at:11-Jul-2019 22:58:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 22:58:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.075

PFS not locked. Waiting for lock...

PFS took 0.400000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 22:58:58

Channel:Brightfield set at:11-Jul-2019 22:58:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 22:58:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:58:58

Channel configuration set to: GFP: 11-Jul-2019 22:59:00

Channel:GFP set at:11-Jul-2019 22:59:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 22:59:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:59:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 22:59:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.15

Channel configuration set to: Brightfield: 11-Jul-2019 22:59:02

Channel:Brightfield set at:11-Jul-2019 22:59:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 11-Jul-2019 22:59:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:59:03

Channel configuration set to: GFP: 11-Jul-2019 22:59:04

Channel:GFP set at:11-Jul-2019 22:59:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 11-Jul-2019 22:59:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 22:59:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:79 completed at:11-Jul-2019 22:59:06

Time since start of timelapse:23492.7737

PFS is locked

Time to next time point:207.8267



------Time point_80------

Memory available for all arrays: 61098369024

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:02:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.275

Channel configuration set to: Brightfield: 11-Jul-2019 23:02:36

Channel:Brightfield set at:11-Jul-2019 23:02:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 23:02:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:02:36

Channel configuration set to: GFP: 11-Jul-2019 23:02:38

Channel:GFP set at:11-Jul-2019 23:02:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 23:02:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:02:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:02:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.675

Channel configuration set to: Brightfield: 11-Jul-2019 23:02:41

Channel:Brightfield set at:11-Jul-2019 23:02:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 23:02:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:02:41

Channel configuration set to: GFP: 11-Jul-2019 23:02:42

Channel:GFP set at:11-Jul-2019 23:02:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 23:02:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:02:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:02:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10

Channel configuration set to: Brightfield: 11-Jul-2019 23:02:46

Channel:Brightfield set at:11-Jul-2019 23:02:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 23:02:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:02:46

Channel configuration set to: GFP: 11-Jul-2019 23:02:47

Channel:GFP set at:11-Jul-2019 23:02:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 23:02:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:02:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:02:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.625

Channel configuration set to: Brightfield: 11-Jul-2019 23:02:51

Channel:Brightfield set at:11-Jul-2019 23:02:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 11-Jul-2019 23:02:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:02:51

Channel configuration set to: GFP: 11-Jul-2019 23:02:52

Channel:GFP set at:11-Jul-2019 23:02:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 23:02:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:02:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:02:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.725

Channel configuration set to: Brightfield: 11-Jul-2019 23:02:56

Channel:Brightfield set at:11-Jul-2019 23:02:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 23:02:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:02:56

Channel configuration set to: GFP: 11-Jul-2019 23:02:58

Channel:GFP set at:11-Jul-2019 23:02:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 23:02:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:02:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.35

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:01

Channel:Brightfield set at:11-Jul-2019 23:03:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 23:03:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:01

Channel configuration set to: GFP: 11-Jul-2019 23:03:03

Channel:GFP set at:11-Jul-2019 23:03:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 23:03:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:07

Channel:Brightfield set at:11-Jul-2019 23:03:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 23:03:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:07

Channel configuration set to: GFP: 11-Jul-2019 23:03:08

Channel:GFP set at:11-Jul-2019 23:03:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 23:03:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.5

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:12

Channel:Brightfield set at:11-Jul-2019 23:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 23:03:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:13

Channel configuration set to: GFP: 11-Jul-2019 23:03:14

Channel:GFP set at:11-Jul-2019 23:03:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 23:03:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:18

Channel:Brightfield set at:11-Jul-2019 23:03:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 23:03:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:18

Channel configuration set to: GFP: 11-Jul-2019 23:03:19

Channel:GFP set at:11-Jul-2019 23:03:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 23:03:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:23

Channel:Brightfield set at:11-Jul-2019 23:03:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 11-Jul-2019 23:03:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:23

Channel configuration set to: GFP: 11-Jul-2019 23:03:24

Channel:GFP set at:11-Jul-2019 23:03:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 11-Jul-2019 23:03:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:28

Channel:Brightfield set at:11-Jul-2019 23:03:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 23:03:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:28

Channel configuration set to: GFP: 11-Jul-2019 23:03:29

Channel:GFP set at:11-Jul-2019 23:03:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 11-Jul-2019 23:03:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:34

Channel:Brightfield set at:11-Jul-2019 23:03:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 23:03:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:34

Channel configuration set to: GFP: 11-Jul-2019 23:03:35

Channel:GFP set at:11-Jul-2019 23:03:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 23:03:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:39

Channel:Brightfield set at:11-Jul-2019 23:03:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 11-Jul-2019 23:03:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:40

Channel configuration set to: GFP: 11-Jul-2019 23:03:41

Channel:GFP set at:11-Jul-2019 23:03:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 23:03:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:44

Channel:Brightfield set at:11-Jul-2019 23:03:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 23:03:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:44

Channel configuration set to: GFP: 11-Jul-2019 23:03:46

Channel:GFP set at:11-Jul-2019 23:03:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 23:03:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.825

PFS not locked. Waiting for lock...

PFS took 0.150000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:50

Channel:Brightfield set at:11-Jul-2019 23:03:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 23:03:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:50

Channel configuration set to: GFP: 11-Jul-2019 23:03:51

Channel:GFP set at:11-Jul-2019 23:03:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 23:03:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:55

Channel:Brightfield set at:11-Jul-2019 23:03:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 23:03:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:55

Channel configuration set to: GFP: 11-Jul-2019 23:03:56

Channel:GFP set at:11-Jul-2019 23:03:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 23:03:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:03:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:03:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 23:04:00

Channel:Brightfield set at:11-Jul-2019 23:04:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 23:04:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:04:00

Channel configuration set to: GFP: 11-Jul-2019 23:04:01

Channel:GFP set at:11-Jul-2019 23:04:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 23:04:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:04:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:04:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 11-Jul-2019 23:04:05

Channel:Brightfield set at:11-Jul-2019 23:04:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 23:04:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:04:05

Channel configuration set to: GFP: 11-Jul-2019 23:04:06

Channel:GFP set at:11-Jul-2019 23:04:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 23:04:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:04:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:80 completed at:11-Jul-2019 23:04:09

Time since start of timelapse:23794.9136

PFS is locked

Time to next time point:205.6882



------Time point_81------

Memory available for all arrays: 61099089920

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:07:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 11-Jul-2019 23:07:36

Channel:Brightfield set at:11-Jul-2019 23:07:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 23:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:07:36

Channel configuration set to: GFP: 11-Jul-2019 23:07:38

Channel:GFP set at:11-Jul-2019 23:07:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 23:07:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:07:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:07:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.425

Channel configuration set to: Brightfield: 11-Jul-2019 23:07:41

Channel:Brightfield set at:11-Jul-2019 23:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 23:07:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:07:41

Channel configuration set to: GFP: 11-Jul-2019 23:07:43

Channel:GFP set at:11-Jul-2019 23:07:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 11-Jul-2019 23:07:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:07:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:07:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 11-Jul-2019 23:07:46

Channel:Brightfield set at:11-Jul-2019 23:07:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6501. 11-Jul-2019 23:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:07:46

Channel configuration set to: GFP: 11-Jul-2019 23:07:47

Channel:GFP set at:11-Jul-2019 23:07:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6501. 11-Jul-2019 23:07:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:07:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:07:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.625

Channel configuration set to: Brightfield: 11-Jul-2019 23:07:51

Channel:Brightfield set at:11-Jul-2019 23:07:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 11-Jul-2019 23:07:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:07:52

Channel configuration set to: GFP: 11-Jul-2019 23:07:53

Channel:GFP set at:11-Jul-2019 23:07:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 11-Jul-2019 23:07:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:07:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:07:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.775

Channel configuration set to: Brightfield: 11-Jul-2019 23:07:56

Channel:Brightfield set at:11-Jul-2019 23:07:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 23:07:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:07:57

Channel configuration set to: GFP: 11-Jul-2019 23:07:58

Channel:GFP set at:11-Jul-2019 23:07:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 23:07:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:07:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:02

Channel:Brightfield set at:11-Jul-2019 23:08:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 23:08:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:02

Channel configuration set to: GFP: 11-Jul-2019 23:08:03

Channel:GFP set at:11-Jul-2019 23:08:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 23:08:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:07

Channel:Brightfield set at:11-Jul-2019 23:08:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 23:08:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:07

Channel configuration set to: GFP: 11-Jul-2019 23:08:08

Channel:GFP set at:11-Jul-2019 23:08:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 23:08:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:12

Channel:Brightfield set at:11-Jul-2019 23:08:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 23:08:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:13

Channel configuration set to: GFP: 11-Jul-2019 23:08:14

Channel:GFP set at:11-Jul-2019 23:08:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 23:08:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:18

Channel:Brightfield set at:11-Jul-2019 23:08:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 23:08:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:18

Channel configuration set to: GFP: 11-Jul-2019 23:08:19

Channel:GFP set at:11-Jul-2019 23:08:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 23:08:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:23

Channel:Brightfield set at:11-Jul-2019 23:08:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 11-Jul-2019 23:08:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:23

Channel configuration set to: GFP: 11-Jul-2019 23:08:24

Channel:GFP set at:11-Jul-2019 23:08:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 11-Jul-2019 23:08:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:28

Channel:Brightfield set at:11-Jul-2019 23:08:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 23:08:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:29

Channel configuration set to: GFP: 11-Jul-2019 23:08:30

Channel:GFP set at:11-Jul-2019 23:08:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 23:08:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:34

Channel:Brightfield set at:11-Jul-2019 23:08:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 23:08:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:34

Channel configuration set to: GFP: 11-Jul-2019 23:08:36

Channel:GFP set at:11-Jul-2019 23:08:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 11-Jul-2019 23:08:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:39

Channel:Brightfield set at:11-Jul-2019 23:08:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 11-Jul-2019 23:08:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:40

Channel configuration set to: GFP: 11-Jul-2019 23:08:41

Channel:GFP set at:11-Jul-2019 23:08:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 11-Jul-2019 23:08:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:44

Channel:Brightfield set at:11-Jul-2019 23:08:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 23:08:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:44

Channel configuration set to: GFP: 11-Jul-2019 23:08:46

Channel:GFP set at:11-Jul-2019 23:08:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 23:08:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:49

Channel:Brightfield set at:11-Jul-2019 23:08:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 23:08:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:49

Channel configuration set to: GFP: 11-Jul-2019 23:08:51

Channel:GFP set at:11-Jul-2019 23:08:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 23:08:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:54

Channel:Brightfield set at:11-Jul-2019 23:08:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 23:08:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:54

Channel configuration set to: GFP: 11-Jul-2019 23:08:56

Channel:GFP set at:11-Jul-2019 23:08:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 23:08:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 23:08:59

Channel:Brightfield set at:11-Jul-2019 23:08:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 23:08:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:08:59

Channel configuration set to: GFP: 11-Jul-2019 23:09:01

Channel:GFP set at:11-Jul-2019 23:09:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 23:09:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:09:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:09:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 23:09:04

Channel:Brightfield set at:11-Jul-2019 23:09:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3001. 11-Jul-2019 23:09:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:09:05

Channel configuration set to: GFP: 11-Jul-2019 23:09:06

Channel:GFP set at:11-Jul-2019 23:09:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 11-Jul-2019 23:09:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:09:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:81 completed at:11-Jul-2019 23:09:08

Time since start of timelapse:24094.3096

PFS is locked

Time to next time point:206.2936



------Time point_82------

Memory available for all arrays: 61093892096

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:12:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 11-Jul-2019 23:12:36

Channel:Brightfield set at:11-Jul-2019 23:12:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 23:12:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:12:36

Channel configuration set to: GFP: 11-Jul-2019 23:12:38

Channel:GFP set at:11-Jul-2019 23:12:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 11-Jul-2019 23:12:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:12:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:12:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 11-Jul-2019 23:12:41

Channel:Brightfield set at:11-Jul-2019 23:12:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 23:12:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:12:41

Channel configuration set to: GFP: 11-Jul-2019 23:12:43

Channel:GFP set at:11-Jul-2019 23:12:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 23:12:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:12:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:12:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 11-Jul-2019 23:12:46

Channel:Brightfield set at:11-Jul-2019 23:12:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 11-Jul-2019 23:12:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:12:46

Channel configuration set to: GFP: 11-Jul-2019 23:12:48

Channel:GFP set at:11-Jul-2019 23:12:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 11-Jul-2019 23:12:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:12:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:12:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 11-Jul-2019 23:12:51

Channel:Brightfield set at:11-Jul-2019 23:12:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 23:12:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:12:52

Channel configuration set to: GFP: 11-Jul-2019 23:12:53

Channel:GFP set at:11-Jul-2019 23:12:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 23:12:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:12:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:12:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 11-Jul-2019 23:12:56

Channel:Brightfield set at:11-Jul-2019 23:12:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:12:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:12:57

Channel configuration set to: GFP: 11-Jul-2019 23:12:58

Channel:GFP set at:11-Jul-2019 23:12:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:12:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:12:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:01

Channel:Brightfield set at:11-Jul-2019 23:13:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 11-Jul-2019 23:13:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:02

Channel configuration set to: GFP: 11-Jul-2019 23:13:03

Channel:GFP set at:11-Jul-2019 23:13:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 11-Jul-2019 23:13:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:07

Channel:Brightfield set at:11-Jul-2019 23:13:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 23:13:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:07

Channel configuration set to: GFP: 11-Jul-2019 23:13:08

Channel:GFP set at:11-Jul-2019 23:13:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 11-Jul-2019 23:13:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:11

Channel:Brightfield set at:11-Jul-2019 23:13:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 23:13:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:12

Channel configuration set to: GFP: 11-Jul-2019 23:13:13

Channel:GFP set at:11-Jul-2019 23:13:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 23:13:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:16

Channel:Brightfield set at:11-Jul-2019 23:13:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 23:13:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:17

Channel configuration set to: GFP: 11-Jul-2019 23:13:18

Channel:GFP set at:11-Jul-2019 23:13:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 11-Jul-2019 23:13:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:22

Channel:Brightfield set at:11-Jul-2019 23:13:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 11-Jul-2019 23:13:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:22

Channel configuration set to: GFP: 11-Jul-2019 23:13:23

Channel:GFP set at:11-Jul-2019 23:13:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 11-Jul-2019 23:13:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:27

Channel:Brightfield set at:11-Jul-2019 23:13:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 11-Jul-2019 23:13:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:27

Channel configuration set to: GFP: 11-Jul-2019 23:13:28

Channel:GFP set at:11-Jul-2019 23:13:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 11-Jul-2019 23:13:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:33

Channel:Brightfield set at:11-Jul-2019 23:13:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 11-Jul-2019 23:13:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:33

Channel configuration set to: GFP: 11-Jul-2019 23:13:34

Channel:GFP set at:11-Jul-2019 23:13:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 11-Jul-2019 23:13:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.2

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:37

Channel:Brightfield set at:11-Jul-2019 23:13:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 23:13:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:38

Channel configuration set to: GFP: 11-Jul-2019 23:13:39

Channel:GFP set at:11-Jul-2019 23:13:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 23:13:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:42

Channel:Brightfield set at:11-Jul-2019 23:13:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 23:13:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:42

Channel configuration set to: GFP: 11-Jul-2019 23:13:44

Channel:GFP set at:11-Jul-2019 23:13:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 23:13:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:47

Channel:Brightfield set at:11-Jul-2019 23:13:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 23:13:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:48

Channel configuration set to: GFP: 11-Jul-2019 23:13:49

Channel:GFP set at:11-Jul-2019 23:13:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 23:13:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:53

Channel:Brightfield set at:11-Jul-2019 23:13:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 23:13:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:53

Channel configuration set to: GFP: 11-Jul-2019 23:13:54

Channel:GFP set at:11-Jul-2019 23:13:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 23:13:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 11-Jul-2019 23:13:58

Channel:Brightfield set at:11-Jul-2019 23:13:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 11-Jul-2019 23:13:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:13:58

Channel configuration set to: GFP: 11-Jul-2019 23:13:59

Channel:GFP set at:11-Jul-2019 23:13:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 11-Jul-2019 23:14:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:14:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:14:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 23:14:03

Channel:Brightfield set at:11-Jul-2019 23:14:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 23:14:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:14:03

Channel configuration set to: GFP: 11-Jul-2019 23:14:04

Channel:GFP set at:11-Jul-2019 23:14:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 11-Jul-2019 23:14:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:14:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:82 completed at:11-Jul-2019 23:14:07

Time since start of timelapse:24392.8999

PFS is locked

Time to next time point:207.7048



------Time point_83------

Memory available for all arrays: 61096058880

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:17:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 11-Jul-2019 23:17:36

Channel:Brightfield set at:11-Jul-2019 23:17:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 23:17:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:17:36

Channel configuration set to: GFP: 11-Jul-2019 23:17:38

Channel:GFP set at:11-Jul-2019 23:17:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 11-Jul-2019 23:17:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:17:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:17:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.45

Channel configuration set to: Brightfield: 11-Jul-2019 23:17:40

Channel:Brightfield set at:11-Jul-2019 23:17:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 23:17:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:17:41

Channel configuration set to: GFP: 11-Jul-2019 23:17:42

Channel:GFP set at:11-Jul-2019 23:17:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 11-Jul-2019 23:17:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:17:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:17:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 11-Jul-2019 23:17:45

Channel:Brightfield set at:11-Jul-2019 23:17:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 23:17:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:17:45

Channel configuration set to: GFP: 11-Jul-2019 23:17:47

Channel:GFP set at:11-Jul-2019 23:17:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 23:17:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:17:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:17:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 11-Jul-2019 23:17:51

Channel:Brightfield set at:11-Jul-2019 23:17:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 23:17:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:17:51

Channel configuration set to: GFP: 11-Jul-2019 23:17:52

Channel:GFP set at:11-Jul-2019 23:17:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 23:17:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:17:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:17:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.6

Channel configuration set to: Brightfield: 11-Jul-2019 23:17:56

Channel:Brightfield set at:11-Jul-2019 23:17:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 23:17:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:17:56

Channel configuration set to: GFP: 11-Jul-2019 23:17:58

Channel:GFP set at:11-Jul-2019 23:17:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 23:17:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:17:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.775

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:01

Channel:Brightfield set at:11-Jul-2019 23:18:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 23:18:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:01

Channel configuration set to: GFP: 11-Jul-2019 23:18:02

Channel:GFP set at:11-Jul-2019 23:18:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 23:18:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:07

Channel:Brightfield set at:11-Jul-2019 23:18:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 23:18:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:07

Channel configuration set to: GFP: 11-Jul-2019 23:18:08

Channel:GFP set at:11-Jul-2019 23:18:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 23:18:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:12

Channel:Brightfield set at:11-Jul-2019 23:18:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 23:18:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:12

Channel configuration set to: GFP: 11-Jul-2019 23:18:13

Channel:GFP set at:11-Jul-2019 23:18:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 23:18:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:16

Channel:Brightfield set at:11-Jul-2019 23:18:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 23:18:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:17

Channel configuration set to: GFP: 11-Jul-2019 23:18:18

Channel:GFP set at:11-Jul-2019 23:18:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 23:18:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:21

Channel:Brightfield set at:11-Jul-2019 23:18:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 11-Jul-2019 23:18:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:22

Channel configuration set to: GFP: 11-Jul-2019 23:18:23

Channel:GFP set at:11-Jul-2019 23:18:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 11-Jul-2019 23:18:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:26

Channel:Brightfield set at:11-Jul-2019 23:18:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 23:18:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:27

Channel configuration set to: GFP: 11-Jul-2019 23:18:28

Channel:GFP set at:11-Jul-2019 23:18:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 11-Jul-2019 23:18:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:31

Channel:Brightfield set at:11-Jul-2019 23:18:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 11-Jul-2019 23:18:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:32

Channel configuration set to: GFP: 11-Jul-2019 23:18:33

Channel:GFP set at:11-Jul-2019 23:18:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 11-Jul-2019 23:18:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:37

Channel:Brightfield set at:11-Jul-2019 23:18:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 11-Jul-2019 23:18:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:37

Channel configuration set to: GFP: 11-Jul-2019 23:18:38

Channel:GFP set at:11-Jul-2019 23:18:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 11-Jul-2019 23:18:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:42

Channel:Brightfield set at:11-Jul-2019 23:18:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 23:18:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:42

Channel configuration set to: GFP: 11-Jul-2019 23:18:44

Channel:GFP set at:11-Jul-2019 23:18:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 23:18:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:47

Channel:Brightfield set at:11-Jul-2019 23:18:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 23:18:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:47

Channel configuration set to: GFP: 11-Jul-2019 23:18:49

Channel:GFP set at:11-Jul-2019 23:18:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 23:18:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:52

Channel:Brightfield set at:11-Jul-2019 23:18:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7751. 11-Jul-2019 23:18:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:53

Channel configuration set to: GFP: 11-Jul-2019 23:18:54

Channel:GFP set at:11-Jul-2019 23:18:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 11-Jul-2019 23:18:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 11-Jul-2019 23:18:57

Channel:Brightfield set at:11-Jul-2019 23:18:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 23:18:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:57

Channel configuration set to: GFP: 11-Jul-2019 23:18:58

Channel:GFP set at:11-Jul-2019 23:18:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 23:18:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:18:59

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:19:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.925

Channel configuration set to: Brightfield: 11-Jul-2019 23:19:02

Channel:Brightfield set at:11-Jul-2019 23:19:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 23:19:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:19:02

Channel configuration set to: GFP: 11-Jul-2019 23:19:04

Channel:GFP set at:11-Jul-2019 23:19:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 23:19:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:19:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:83 completed at:11-Jul-2019 23:19:06

Time since start of timelapse:24692.1416

PFS is locked

Time to next time point:208.4648



------Time point_84------

Memory available for all arrays: 61096357888

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:22:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.3

Channel configuration set to: Brightfield: 11-Jul-2019 23:22:36

Channel:Brightfield set at:11-Jul-2019 23:22:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 23:22:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:22:36

Channel configuration set to: GFP: 11-Jul-2019 23:22:38

Channel:GFP set at:11-Jul-2019 23:22:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 23:22:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:22:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:22:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.4

Channel configuration set to: Brightfield: 11-Jul-2019 23:22:41

Channel:Brightfield set at:11-Jul-2019 23:22:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 23:22:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:22:41

Channel configuration set to: GFP: 11-Jul-2019 23:22:43

Channel:GFP set at:11-Jul-2019 23:22:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 23:22:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:22:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:22:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 23:22:45

Channel:Brightfield set at:11-Jul-2019 23:22:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 11-Jul-2019 23:22:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:22:46

Channel configuration set to: GFP: 11-Jul-2019 23:22:47

Channel:GFP set at:11-Jul-2019 23:22:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 11-Jul-2019 23:22:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:22:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:22:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.525

PFS not locked. Waiting for lock...

PFS took 0.300000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 23:22:51

Channel:Brightfield set at:11-Jul-2019 23:22:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 23:22:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:22:51

Channel configuration set to: GFP: 11-Jul-2019 23:22:52

Channel:GFP set at:11-Jul-2019 23:22:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 23:22:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:22:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:22:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.75

Channel configuration set to: Brightfield: 11-Jul-2019 23:22:56

Channel:Brightfield set at:11-Jul-2019 23:22:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 23:22:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:22:56

Channel configuration set to: GFP: 11-Jul-2019 23:22:58

Channel:GFP set at:11-Jul-2019 23:22:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 23:22:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:22:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:01

Channel:Brightfield set at:11-Jul-2019 23:23:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:23:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:02

Channel configuration set to: GFP: 11-Jul-2019 23:23:03

Channel:GFP set at:11-Jul-2019 23:23:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:23:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:06

Channel:Brightfield set at:11-Jul-2019 23:23:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 23:23:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:06

Channel configuration set to: GFP: 11-Jul-2019 23:23:08

Channel:GFP set at:11-Jul-2019 23:23:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 23:23:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:12

Channel:Brightfield set at:11-Jul-2019 23:23:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 23:23:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:12

Channel configuration set to: GFP: 11-Jul-2019 23:23:13

Channel:GFP set at:11-Jul-2019 23:23:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 11-Jul-2019 23:23:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:17

Channel:Brightfield set at:11-Jul-2019 23:23:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 23:23:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:17

Channel configuration set to: GFP: 11-Jul-2019 23:23:19

Channel:GFP set at:11-Jul-2019 23:23:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 11-Jul-2019 23:23:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:22

Channel:Brightfield set at:11-Jul-2019 23:23:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 23:23:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:22

Channel configuration set to: GFP: 11-Jul-2019 23:23:24

Channel:GFP set at:11-Jul-2019 23:23:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 23:23:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:27

Channel:Brightfield set at:11-Jul-2019 23:23:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 23:23:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:27

Channel configuration set to: GFP: 11-Jul-2019 23:23:29

Channel:GFP set at:11-Jul-2019 23:23:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 23:23:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:32

Channel:Brightfield set at:11-Jul-2019 23:23:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 11-Jul-2019 23:23:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:32

Channel configuration set to: GFP: 11-Jul-2019 23:23:34

Channel:GFP set at:11-Jul-2019 23:23:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 11-Jul-2019 23:23:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:37

Channel:Brightfield set at:11-Jul-2019 23:23:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 23:23:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:37

Channel configuration set to: GFP: 11-Jul-2019 23:23:39

Channel:GFP set at:11-Jul-2019 23:23:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 23:23:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:42

Channel:Brightfield set at:11-Jul-2019 23:23:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 23:23:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:42

Channel configuration set to: GFP: 11-Jul-2019 23:23:44

Channel:GFP set at:11-Jul-2019 23:23:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 23:23:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:47

Channel:Brightfield set at:11-Jul-2019 23:23:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 23:23:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:47

Channel configuration set to: GFP: 11-Jul-2019 23:23:49

Channel:GFP set at:11-Jul-2019 23:23:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 11-Jul-2019 23:23:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:52

Channel:Brightfield set at:11-Jul-2019 23:23:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 23:23:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:53

Channel configuration set to: GFP: 11-Jul-2019 23:23:54

Channel:GFP set at:11-Jul-2019 23:23:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 23:23:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.225

PFS not locked. Waiting for lock...

PFS took 0.450000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 23:23:57

Channel:Brightfield set at:11-Jul-2019 23:23:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 23:23:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:57

Channel configuration set to: GFP: 11-Jul-2019 23:23:59

Channel:GFP set at:11-Jul-2019 23:23:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 11-Jul-2019 23:23:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:23:59

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:24:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 11-Jul-2019 23:24:02

Channel:Brightfield set at:11-Jul-2019 23:24:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 23:24:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:24:03

Channel configuration set to: GFP: 11-Jul-2019 23:24:04

Channel:GFP set at:11-Jul-2019 23:24:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 23:24:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:24:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:84 completed at:11-Jul-2019 23:24:06

Time since start of timelapse:24992.3435

PFS is locked

Time to next time point:208.2642



------Time point_85------

Memory available for all arrays: 61098250240

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:27:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 11-Jul-2019 23:27:36

Channel:Brightfield set at:11-Jul-2019 23:27:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 23:27:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:27:36

Channel configuration set to: GFP: 11-Jul-2019 23:27:37

Channel:GFP set at:11-Jul-2019 23:27:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 11-Jul-2019 23:27:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:27:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:27:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 11-Jul-2019 23:27:41

Channel:Brightfield set at:11-Jul-2019 23:27:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 23:27:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:27:41

Channel configuration set to: GFP: 11-Jul-2019 23:27:43

Channel:GFP set at:11-Jul-2019 23:27:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 23:27:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:27:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:27:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 11-Jul-2019 23:27:46

Channel:Brightfield set at:11-Jul-2019 23:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 11-Jul-2019 23:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:27:46

Channel configuration set to: GFP: 11-Jul-2019 23:27:48

Channel:GFP set at:11-Jul-2019 23:27:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 11-Jul-2019 23:27:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:27:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:27:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.675

Channel configuration set to: Brightfield: 11-Jul-2019 23:27:51

Channel:Brightfield set at:11-Jul-2019 23:27:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 11-Jul-2019 23:27:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:27:52

Channel configuration set to: GFP: 11-Jul-2019 23:27:53

Channel:GFP set at:11-Jul-2019 23:27:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 11-Jul-2019 23:27:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:27:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:27:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.875

Channel configuration set to: Brightfield: 11-Jul-2019 23:27:56

Channel:Brightfield set at:11-Jul-2019 23:27:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 23:27:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:27:56

Channel configuration set to: GFP: 11-Jul-2019 23:27:57

Channel:GFP set at:11-Jul-2019 23:27:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 23:27:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:27:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:01

Channel:Brightfield set at:11-Jul-2019 23:28:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:28:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:01

Channel configuration set to: GFP: 11-Jul-2019 23:28:03

Channel:GFP set at:11-Jul-2019 23:28:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:28:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:07

Channel:Brightfield set at:11-Jul-2019 23:28:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 11-Jul-2019 23:28:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:07

Channel configuration set to: GFP: 11-Jul-2019 23:28:08

Channel:GFP set at:11-Jul-2019 23:28:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 11-Jul-2019 23:28:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:12

Channel:Brightfield set at:11-Jul-2019 23:28:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 23:28:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:13

Channel configuration set to: GFP: 11-Jul-2019 23:28:14

Channel:GFP set at:11-Jul-2019 23:28:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 23:28:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:18

Channel:Brightfield set at:11-Jul-2019 23:28:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 11-Jul-2019 23:28:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:18

Channel configuration set to: GFP: 11-Jul-2019 23:28:19

Channel:GFP set at:11-Jul-2019 23:28:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 23:28:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:23

Channel:Brightfield set at:11-Jul-2019 23:28:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 11-Jul-2019 23:28:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:23

Channel configuration set to: GFP: 11-Jul-2019 23:28:24

Channel:GFP set at:11-Jul-2019 23:28:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 11-Jul-2019 23:28:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:28

Channel:Brightfield set at:11-Jul-2019 23:28:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 23:28:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:28

Channel configuration set to: GFP: 11-Jul-2019 23:28:29

Channel:GFP set at:11-Jul-2019 23:28:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 23:28:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:33

Channel:Brightfield set at:11-Jul-2019 23:28:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 11-Jul-2019 23:28:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:33

Channel configuration set to: GFP: 11-Jul-2019 23:28:34

Channel:GFP set at:11-Jul-2019 23:28:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 11-Jul-2019 23:28:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:38

Channel:Brightfield set at:11-Jul-2019 23:28:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 11-Jul-2019 23:28:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:39

Channel configuration set to: GFP: 11-Jul-2019 23:28:40

Channel:GFP set at:11-Jul-2019 23:28:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 11-Jul-2019 23:28:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:44

Channel:Brightfield set at:11-Jul-2019 23:28:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 23:28:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:44

Channel configuration set to: GFP: 11-Jul-2019 23:28:45

Channel:GFP set at:11-Jul-2019 23:28:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 23:28:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:49

Channel:Brightfield set at:11-Jul-2019 23:28:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 23:28:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:49

Channel configuration set to: GFP: 11-Jul-2019 23:28:50

Channel:GFP set at:11-Jul-2019 23:28:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 11-Jul-2019 23:28:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:54

Channel:Brightfield set at:11-Jul-2019 23:28:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 23:28:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:54

Channel configuration set to: GFP: 11-Jul-2019 23:28:55

Channel:GFP set at:11-Jul-2019 23:28:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 23:28:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 11-Jul-2019 23:28:59

Channel:Brightfield set at:11-Jul-2019 23:28:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 23:28:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:28:59

Channel configuration set to: GFP: 11-Jul-2019 23:29:00

Channel:GFP set at:11-Jul-2019 23:29:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 11-Jul-2019 23:29:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:29:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:29:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 11-Jul-2019 23:29:03

Channel:Brightfield set at:11-Jul-2019 23:29:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 23:29:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:29:04

Channel configuration set to: GFP: 11-Jul-2019 23:29:05

Channel:GFP set at:11-Jul-2019 23:29:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 11-Jul-2019 23:29:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:29:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:85 completed at:11-Jul-2019 23:29:07

Time since start of timelapse:25293.6928

PFS is locked

Time to next time point:206.9167



------Time point_86------

Memory available for all arrays: 61097250816

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:32:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.1

Channel configuration set to: Brightfield: 11-Jul-2019 23:32:36

Channel:Brightfield set at:11-Jul-2019 23:32:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1501. 11-Jul-2019 23:32:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:32:36

Channel configuration set to: GFP: 11-Jul-2019 23:32:37

Channel:GFP set at:11-Jul-2019 23:32:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1501. 11-Jul-2019 23:32:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:32:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:32:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 11-Jul-2019 23:32:41

Channel:Brightfield set at:11-Jul-2019 23:32:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 23:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:32:41

Channel configuration set to: GFP: 11-Jul-2019 23:32:42

Channel:GFP set at:11-Jul-2019 23:32:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 11-Jul-2019 23:32:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:32:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:32:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 11-Jul-2019 23:32:45

Channel:Brightfield set at:11-Jul-2019 23:32:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 11-Jul-2019 23:32:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:32:46

Channel configuration set to: GFP: 11-Jul-2019 23:32:47

Channel:GFP set at:11-Jul-2019 23:32:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 11-Jul-2019 23:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:32:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:32:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 11-Jul-2019 23:32:51

Channel:Brightfield set at:11-Jul-2019 23:32:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 23:32:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:32:51

Channel configuration set to: GFP: 11-Jul-2019 23:32:53

Channel:GFP set at:11-Jul-2019 23:32:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 23:32:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:32:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:32:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 11-Jul-2019 23:32:56

Channel:Brightfield set at:11-Jul-2019 23:32:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:32:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:32:57

Channel configuration set to: GFP: 11-Jul-2019 23:32:58

Channel:GFP set at:11-Jul-2019 23:32:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:32:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:32:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.225

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:01

Channel:Brightfield set at:11-Jul-2019 23:33:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 23:33:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:01

Channel configuration set to: GFP: 11-Jul-2019 23:33:02

Channel:GFP set at:11-Jul-2019 23:33:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 11-Jul-2019 23:33:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:06

Channel:Brightfield set at:11-Jul-2019 23:33:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 11-Jul-2019 23:33:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:06

Channel configuration set to: GFP: 11-Jul-2019 23:33:08

Channel:GFP set at:11-Jul-2019 23:33:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 11-Jul-2019 23:33:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.425

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:12

Channel:Brightfield set at:11-Jul-2019 23:33:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 11-Jul-2019 23:33:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:12

Channel configuration set to: GFP: 11-Jul-2019 23:33:13

Channel:GFP set at:11-Jul-2019 23:33:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5001. 11-Jul-2019 23:33:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:16

Channel:Brightfield set at:11-Jul-2019 23:33:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 23:33:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:17

Channel configuration set to: GFP: 11-Jul-2019 23:33:18

Channel:GFP set at:11-Jul-2019 23:33:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 23:33:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:22

Channel:Brightfield set at:11-Jul-2019 23:33:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 23:33:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:22

Channel configuration set to: GFP: 11-Jul-2019 23:33:24

Channel:GFP set at:11-Jul-2019 23:33:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 11-Jul-2019 23:33:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:27

Channel:Brightfield set at:11-Jul-2019 23:33:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 23:33:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:28

Channel configuration set to: GFP: 11-Jul-2019 23:33:29

Channel:GFP set at:11-Jul-2019 23:33:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 11-Jul-2019 23:33:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:32

Channel:Brightfield set at:11-Jul-2019 23:33:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 11-Jul-2019 23:33:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:32

Channel configuration set to: GFP: 11-Jul-2019 23:33:34

Channel:GFP set at:11-Jul-2019 23:33:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 11-Jul-2019 23:33:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:37

Channel:Brightfield set at:11-Jul-2019 23:33:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 23:33:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:37

Channel configuration set to: GFP: 11-Jul-2019 23:33:39

Channel:GFP set at:11-Jul-2019 23:33:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 23:33:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:42

Channel:Brightfield set at:11-Jul-2019 23:33:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 23:33:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:42

Channel configuration set to: GFP: 11-Jul-2019 23:33:43

Channel:GFP set at:11-Jul-2019 23:33:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 23:33:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:46

Channel:Brightfield set at:11-Jul-2019 23:33:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 23:33:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:47

Channel configuration set to: GFP: 11-Jul-2019 23:33:48

Channel:GFP set at:11-Jul-2019 23:33:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 23:33:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.7

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:52

Channel:Brightfield set at:11-Jul-2019 23:33:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 23:33:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:53

Channel configuration set to: GFP: 11-Jul-2019 23:33:54

Channel:GFP set at:11-Jul-2019 23:33:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 23:33:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.35

Channel configuration set to: Brightfield: 11-Jul-2019 23:33:57

Channel:Brightfield set at:11-Jul-2019 23:33:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 23:33:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:58

Channel configuration set to: GFP: 11-Jul-2019 23:33:59

Channel:GFP set at:11-Jul-2019 23:33:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 23:33:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:33:59

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:34:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 23:34:02

Channel:Brightfield set at:11-Jul-2019 23:34:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 23:34:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:34:03

Channel configuration set to: GFP: 11-Jul-2019 23:34:04

Channel:GFP set at:11-Jul-2019 23:34:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 23:34:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:34:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:86 completed at:11-Jul-2019 23:34:06

Time since start of timelapse:25592.6783

PFS is locked

Time to next time point:207.9329



------Time point_87------

Memory available for all arrays: 61096570880

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:37:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 11-Jul-2019 23:37:36

Channel:Brightfield set at:11-Jul-2019 23:37:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 23:37:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:37:36

Channel configuration set to: GFP: 11-Jul-2019 23:37:37

Channel:GFP set at:11-Jul-2019 23:37:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 11-Jul-2019 23:37:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:37:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:37:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 11-Jul-2019 23:37:41

Channel:Brightfield set at:11-Jul-2019 23:37:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 11-Jul-2019 23:37:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:37:41

Channel configuration set to: GFP: 11-Jul-2019 23:37:42

Channel:GFP set at:11-Jul-2019 23:37:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 23:37:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:37:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:37:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 11-Jul-2019 23:37:46

Channel:Brightfield set at:11-Jul-2019 23:37:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 11-Jul-2019 23:37:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:37:46

Channel configuration set to: GFP: 11-Jul-2019 23:37:47

Channel:GFP set at:11-Jul-2019 23:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 11-Jul-2019 23:37:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:37:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:37:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.525

Channel configuration set to: Brightfield: 11-Jul-2019 23:37:51

Channel:Brightfield set at:11-Jul-2019 23:37:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1751. 11-Jul-2019 23:37:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:37:51

Channel configuration set to: GFP: 11-Jul-2019 23:37:52

Channel:GFP set at:11-Jul-2019 23:37:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1501. 11-Jul-2019 23:37:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:37:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:37:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.525

Channel configuration set to: Brightfield: 11-Jul-2019 23:37:55

Channel:Brightfield set at:11-Jul-2019 23:37:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 23:37:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:37:56

Channel configuration set to: GFP: 11-Jul-2019 23:37:57

Channel:GFP set at:11-Jul-2019 23:37:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 23:37:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:37:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.275

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:01

Channel:Brightfield set at:11-Jul-2019 23:38:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 23:38:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:01

Channel configuration set to: GFP: 11-Jul-2019 23:38:03

Channel:GFP set at:11-Jul-2019 23:38:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 11-Jul-2019 23:38:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:06

Channel:Brightfield set at:11-Jul-2019 23:38:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 23:38:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:07

Channel configuration set to: GFP: 11-Jul-2019 23:38:08

Channel:GFP set at:11-Jul-2019 23:38:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 23:38:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.475

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:12

Channel:Brightfield set at:11-Jul-2019 23:38:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4501. 11-Jul-2019 23:38:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:12

Channel configuration set to: GFP: 11-Jul-2019 23:38:14

Channel:GFP set at:11-Jul-2019 23:38:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4501. 11-Jul-2019 23:38:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:17

Channel:Brightfield set at:11-Jul-2019 23:38:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 23:38:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:18

Channel configuration set to: GFP: 11-Jul-2019 23:38:19

Channel:GFP set at:11-Jul-2019 23:38:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 23:38:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:22

Channel:Brightfield set at:11-Jul-2019 23:38:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 23:38:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:22

Channel configuration set to: GFP: 11-Jul-2019 23:38:23

Channel:GFP set at:11-Jul-2019 23:38:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 11-Jul-2019 23:38:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:27

Channel:Brightfield set at:11-Jul-2019 23:38:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 11-Jul-2019 23:38:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:27

Channel configuration set to: GFP: 11-Jul-2019 23:38:28

Channel:GFP set at:11-Jul-2019 23:38:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 11-Jul-2019 23:38:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:32

Channel:Brightfield set at:11-Jul-2019 23:38:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 11-Jul-2019 23:38:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:32

Channel configuration set to: GFP: 11-Jul-2019 23:38:33

Channel:GFP set at:11-Jul-2019 23:38:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 11-Jul-2019 23:38:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:38

Channel:Brightfield set at:11-Jul-2019 23:38:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 23:38:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:38

Channel configuration set to: GFP: 11-Jul-2019 23:38:39

Channel:GFP set at:11-Jul-2019 23:38:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 11-Jul-2019 23:38:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:43

Channel:Brightfield set at:11-Jul-2019 23:38:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 23:38:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:43

Channel configuration set to: GFP: 11-Jul-2019 23:38:45

Channel:GFP set at:11-Jul-2019 23:38:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 23:38:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:48

Channel:Brightfield set at:11-Jul-2019 23:38:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 23:38:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:48

Channel configuration set to: GFP: 11-Jul-2019 23:38:50

Channel:GFP set at:11-Jul-2019 23:38:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 11-Jul-2019 23:38:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:53

Channel:Brightfield set at:11-Jul-2019 23:38:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 23:38:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:53

Channel configuration set to: GFP: 11-Jul-2019 23:38:55

Channel:GFP set at:11-Jul-2019 23:38:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 23:38:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.4

Channel configuration set to: Brightfield: 11-Jul-2019 23:38:58

Channel:Brightfield set at:11-Jul-2019 23:38:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 23:38:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:38:58

Channel configuration set to: GFP: 11-Jul-2019 23:38:59

Channel:GFP set at:11-Jul-2019 23:38:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 11-Jul-2019 23:39:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:39:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:39:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 23:39:03

Channel:Brightfield set at:11-Jul-2019 23:39:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 23:39:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:39:03

Channel configuration set to: GFP: 11-Jul-2019 23:39:05

Channel:GFP set at:11-Jul-2019 23:39:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 23:39:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:39:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:87 completed at:11-Jul-2019 23:39:07

Time since start of timelapse:25893.3255

PFS is locked

Time to next time point:207.2873



------Time point_88------

Memory available for all arrays: 61095993344

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:42:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 11-Jul-2019 23:42:36

Channel:Brightfield set at:11-Jul-2019 23:42:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 11-Jul-2019 23:42:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:42:36

Channel configuration set to: GFP: 11-Jul-2019 23:42:37

Channel:GFP set at:11-Jul-2019 23:42:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 11-Jul-2019 23:42:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:42:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:42:41

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 11-Jul-2019 23:42:42

Channel:Brightfield set at:11-Jul-2019 23:42:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 23:42:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:42:42

Channel configuration set to: GFP: 11-Jul-2019 23:42:43

Channel:GFP set at:11-Jul-2019 23:42:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 11-Jul-2019 23:42:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:42:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:42:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10

Channel configuration set to: Brightfield: 11-Jul-2019 23:42:47

Channel:Brightfield set at:11-Jul-2019 23:42:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 23:42:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:42:47

Channel configuration set to: GFP: 11-Jul-2019 23:42:48

Channel:GFP set at:11-Jul-2019 23:42:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 11-Jul-2019 23:42:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:42:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:42:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.4

Channel configuration set to: Brightfield: 11-Jul-2019 23:42:52

Channel:Brightfield set at:11-Jul-2019 23:42:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1751. 11-Jul-2019 23:42:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:42:53

Channel configuration set to: GFP: 11-Jul-2019 23:42:54

Channel:GFP set at:11-Jul-2019 23:42:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1751. 11-Jul-2019 23:42:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:42:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:42:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.675

Channel configuration set to: Brightfield: 11-Jul-2019 23:42:57

Channel:Brightfield set at:11-Jul-2019 23:42:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 23:42:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:42:58

Channel configuration set to: GFP: 11-Jul-2019 23:42:59

Channel:GFP set at:11-Jul-2019 23:42:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 23:42:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:42:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:02

Channel:Brightfield set at:11-Jul-2019 23:43:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9251. 11-Jul-2019 23:43:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:02

Channel configuration set to: GFP: 11-Jul-2019 23:43:04

Channel:GFP set at:11-Jul-2019 23:43:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9251. 11-Jul-2019 23:43:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:08

Channel:Brightfield set at:11-Jul-2019 23:43:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 23:43:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:08

Channel configuration set to: GFP: 11-Jul-2019 23:43:10

Channel:GFP set at:11-Jul-2019 23:43:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 11-Jul-2019 23:43:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:13

Channel:Brightfield set at:11-Jul-2019 23:43:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 11-Jul-2019 23:43:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:14

Channel configuration set to: GFP: 11-Jul-2019 23:43:15

Channel:GFP set at:11-Jul-2019 23:43:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 11-Jul-2019 23:43:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:18

Channel:Brightfield set at:11-Jul-2019 23:43:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 23:43:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:18

Channel configuration set to: GFP: 11-Jul-2019 23:43:20

Channel:GFP set at:11-Jul-2019 23:43:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 11-Jul-2019 23:43:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:24

Channel:Brightfield set at:11-Jul-2019 23:43:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 23:43:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:24

Channel configuration set to: GFP: 11-Jul-2019 23:43:25

Channel:GFP set at:11-Jul-2019 23:43:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 23:43:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:28

Channel:Brightfield set at:11-Jul-2019 23:43:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 11-Jul-2019 23:43:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:29

Channel configuration set to: GFP: 11-Jul-2019 23:43:30

Channel:GFP set at:11-Jul-2019 23:43:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 11-Jul-2019 23:43:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:33

Channel:Brightfield set at:11-Jul-2019 23:43:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 11-Jul-2019 23:43:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:34

Channel configuration set to: GFP: 11-Jul-2019 23:43:35

Channel:GFP set at:11-Jul-2019 23:43:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 11-Jul-2019 23:43:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:39

Channel:Brightfield set at:11-Jul-2019 23:43:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 11-Jul-2019 23:43:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:40

Channel configuration set to: GFP: 11-Jul-2019 23:43:41

Channel:GFP set at:11-Jul-2019 23:43:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 11-Jul-2019 23:43:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:45

Channel:Brightfield set at:11-Jul-2019 23:43:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 23:43:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:45

Channel configuration set to: GFP: 11-Jul-2019 23:43:46

Channel:GFP set at:11-Jul-2019 23:43:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 23:43:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:49

Channel:Brightfield set at:11-Jul-2019 23:43:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 23:43:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:49

Channel configuration set to: GFP: 11-Jul-2019 23:43:51

Channel:GFP set at:11-Jul-2019 23:43:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 11-Jul-2019 23:43:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:54

Channel:Brightfield set at:11-Jul-2019 23:43:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 11-Jul-2019 23:43:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:54

Channel configuration set to: GFP: 11-Jul-2019 23:43:55

Channel:GFP set at:11-Jul-2019 23:43:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7251. 11-Jul-2019 23:43:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 11-Jul-2019 23:43:58

Channel:Brightfield set at:11-Jul-2019 23:43:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 23:43:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:43:59

Channel configuration set to: GFP: 11-Jul-2019 23:44:00

Channel:GFP set at:11-Jul-2019 23:44:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 23:44:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:44:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:44:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 11-Jul-2019 23:44:04

Channel:Brightfield set at:11-Jul-2019 23:44:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 11-Jul-2019 23:44:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:44:04

Channel configuration set to: GFP: 11-Jul-2019 23:44:06

Channel:GFP set at:11-Jul-2019 23:44:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 11-Jul-2019 23:44:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:44:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:88 completed at:11-Jul-2019 23:44:08

Time since start of timelapse:26194.1283

PFS is locked

Time to next time point:206.4857



------Time point_89------

Memory available for all arrays: 61092196352

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:47:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 11-Jul-2019 23:47:36

Channel:Brightfield set at:11-Jul-2019 23:47:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 11-Jul-2019 23:47:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:47:36

Channel configuration set to: GFP: 11-Jul-2019 23:47:37

Channel:GFP set at:11-Jul-2019 23:47:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8751. 11-Jul-2019 23:47:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:47:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:47:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 11-Jul-2019 23:47:41

Channel:Brightfield set at:11-Jul-2019 23:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 23:47:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:47:42

Channel configuration set to: GFP: 11-Jul-2019 23:47:43

Channel:GFP set at:11-Jul-2019 23:47:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 11-Jul-2019 23:47:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:47:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:47:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.05

Channel configuration set to: Brightfield: 11-Jul-2019 23:47:46

Channel:Brightfield set at:11-Jul-2019 23:47:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 11-Jul-2019 23:47:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:47:47

Channel configuration set to: GFP: 11-Jul-2019 23:47:48

Channel:GFP set at:11-Jul-2019 23:47:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 11-Jul-2019 23:47:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:47:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:47:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 11-Jul-2019 23:47:51

Channel:Brightfield set at:11-Jul-2019 23:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 11-Jul-2019 23:47:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:47:51

Channel configuration set to: GFP: 11-Jul-2019 23:47:53

Channel:GFP set at:11-Jul-2019 23:47:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 11-Jul-2019 23:47:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:47:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:47:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.775

Channel configuration set to: Brightfield: 11-Jul-2019 23:47:56

Channel:Brightfield set at:11-Jul-2019 23:47:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 23:47:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:47:57

Channel configuration set to: GFP: 11-Jul-2019 23:47:58

Channel:GFP set at:11-Jul-2019 23:47:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 23:47:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:47:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:02

Channel:Brightfield set at:11-Jul-2019 23:48:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:48:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:02

Channel configuration set to: GFP: 11-Jul-2019 23:48:03

Channel:GFP set at:11-Jul-2019 23:48:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:48:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:07

Channel:Brightfield set at:11-Jul-2019 23:48:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 23:48:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:07

Channel configuration set to: GFP: 11-Jul-2019 23:48:09

Channel:GFP set at:11-Jul-2019 23:48:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 11-Jul-2019 23:48:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:12

Channel:Brightfield set at:11-Jul-2019 23:48:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 11-Jul-2019 23:48:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:13

Channel configuration set to: GFP: 11-Jul-2019 23:48:14

Channel:GFP set at:11-Jul-2019 23:48:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 11-Jul-2019 23:48:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:18

Channel:Brightfield set at:11-Jul-2019 23:48:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 23:48:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:18

Channel configuration set to: GFP: 11-Jul-2019 23:48:19

Channel:GFP set at:11-Jul-2019 23:48:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 11-Jul-2019 23:48:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:22

Channel:Brightfield set at:11-Jul-2019 23:48:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1001. 11-Jul-2019 23:48:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:23

Channel configuration set to: GFP: 11-Jul-2019 23:48:24

Channel:GFP set at:11-Jul-2019 23:48:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1001. 11-Jul-2019 23:48:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:28

Channel:Brightfield set at:11-Jul-2019 23:48:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 23:48:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:28

Channel configuration set to: GFP: 11-Jul-2019 23:48:29

Channel:GFP set at:11-Jul-2019 23:48:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 23:48:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:32

Channel:Brightfield set at:11-Jul-2019 23:48:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 11-Jul-2019 23:48:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:33

Channel configuration set to: GFP: 11-Jul-2019 23:48:34

Channel:GFP set at:11-Jul-2019 23:48:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 11-Jul-2019 23:48:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:38

Channel:Brightfield set at:11-Jul-2019 23:48:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 11-Jul-2019 23:48:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:39

Channel configuration set to: GFP: 11-Jul-2019 23:48:40

Channel:GFP set at:11-Jul-2019 23:48:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 11-Jul-2019 23:48:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:43

Channel:Brightfield set at:11-Jul-2019 23:48:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 23:48:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:43

Channel configuration set to: GFP: 11-Jul-2019 23:48:45

Channel:GFP set at:11-Jul-2019 23:48:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 11-Jul-2019 23:48:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:48

Channel:Brightfield set at:11-Jul-2019 23:48:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 11-Jul-2019 23:48:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:49

Channel configuration set to: GFP: 11-Jul-2019 23:48:50

Channel:GFP set at:11-Jul-2019 23:48:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 11-Jul-2019 23:48:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:53

Channel:Brightfield set at:11-Jul-2019 23:48:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 11-Jul-2019 23:48:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:53

Channel configuration set to: GFP: 11-Jul-2019 23:48:55

Channel:GFP set at:11-Jul-2019 23:48:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 11-Jul-2019 23:48:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.9

PFS not locked. Waiting for lock...

PFS took 0.450000 seconds to lock.

Channel configuration set to: Brightfield: 11-Jul-2019 23:48:58

Channel:Brightfield set at:11-Jul-2019 23:48:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 23:48:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:48:59

Channel configuration set to: GFP: 11-Jul-2019 23:49:00

Channel:GFP set at:11-Jul-2019 23:49:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 23:49:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:49:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:49:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 11-Jul-2019 23:49:04

Channel:Brightfield set at:11-Jul-2019 23:49:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 23:49:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:49:04

Channel configuration set to: GFP: 11-Jul-2019 23:49:05

Channel:GFP set at:11-Jul-2019 23:49:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 11-Jul-2019 23:49:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:49:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:89 completed at:11-Jul-2019 23:49:07

Time since start of timelapse:26493.8177

PFS is locked

Time to next time point:206.798



------Time point_90------

Memory available for all arrays: 61095108608

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:52:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 11-Jul-2019 23:52:36

Channel:Brightfield set at:11-Jul-2019 23:52:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 23:52:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:52:36

Channel configuration set to: GFP: 11-Jul-2019 23:52:37

Channel:GFP set at:11-Jul-2019 23:52:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 11-Jul-2019 23:52:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:52:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:52:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.4

Channel configuration set to: Brightfield: 11-Jul-2019 23:52:40

Channel:Brightfield set at:11-Jul-2019 23:52:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 23:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:52:41

Channel configuration set to: GFP: 11-Jul-2019 23:52:42

Channel:GFP set at:11-Jul-2019 23:52:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 11-Jul-2019 23:52:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:52:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:52:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.225

Channel configuration set to: Brightfield: 11-Jul-2019 23:52:45

Channel:Brightfield set at:11-Jul-2019 23:52:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6501. 11-Jul-2019 23:52:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:52:46

Channel configuration set to: GFP: 11-Jul-2019 23:52:47

Channel:GFP set at:11-Jul-2019 23:52:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 11-Jul-2019 23:52:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:52:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:52:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.7

Channel configuration set to: Brightfield: 11-Jul-2019 23:52:50

Channel:Brightfield set at:11-Jul-2019 23:52:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 23:52:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:52:51

Channel configuration set to: GFP: 11-Jul-2019 23:52:52

Channel:GFP set at:11-Jul-2019 23:52:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 11-Jul-2019 23:52:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:52:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:52:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.725

Channel configuration set to: Brightfield: 11-Jul-2019 23:52:56

Channel:Brightfield set at:11-Jul-2019 23:52:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 23:52:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:52:56

Channel configuration set to: GFP: 11-Jul-2019 23:52:57

Channel:GFP set at:11-Jul-2019 23:52:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 23:52:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:52:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:00

Channel:Brightfield set at:11-Jul-2019 23:53:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 11-Jul-2019 23:53:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:01

Channel configuration set to: GFP: 11-Jul-2019 23:53:02

Channel:GFP set at:11-Jul-2019 23:53:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 11-Jul-2019 23:53:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:06

Channel:Brightfield set at:11-Jul-2019 23:53:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 11-Jul-2019 23:53:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:07

Channel configuration set to: GFP: 11-Jul-2019 23:53:08

Channel:GFP set at:11-Jul-2019 23:53:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 11-Jul-2019 23:53:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:12

Channel:Brightfield set at:11-Jul-2019 23:53:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 23:53:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:12

Channel configuration set to: GFP: 11-Jul-2019 23:53:13

Channel:GFP set at:11-Jul-2019 23:53:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 23:53:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:17

Channel:Brightfield set at:11-Jul-2019 23:53:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 11-Jul-2019 23:53:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:18

Channel configuration set to: GFP: 11-Jul-2019 23:53:19

Channel:GFP set at:11-Jul-2019 23:53:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 11-Jul-2019 23:53:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:22

Channel:Brightfield set at:11-Jul-2019 23:53:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 23:53:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:22

Channel configuration set to: GFP: 11-Jul-2019 23:53:24

Channel:GFP set at:11-Jul-2019 23:53:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 11-Jul-2019 23:53:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:27

Channel:Brightfield set at:11-Jul-2019 23:53:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 11-Jul-2019 23:53:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:27

Channel configuration set to: GFP: 11-Jul-2019 23:53:29

Channel:GFP set at:11-Jul-2019 23:53:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 11-Jul-2019 23:53:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:32

Channel:Brightfield set at:11-Jul-2019 23:53:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 23:53:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:32

Channel configuration set to: GFP: 11-Jul-2019 23:53:34

Channel:GFP set at:11-Jul-2019 23:53:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 11-Jul-2019 23:53:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:37

Channel:Brightfield set at:11-Jul-2019 23:53:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 23:53:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:37

Channel configuration set to: GFP: 11-Jul-2019 23:53:39

Channel:GFP set at:11-Jul-2019 23:53:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 11-Jul-2019 23:53:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:42

Channel:Brightfield set at:11-Jul-2019 23:53:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:53:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:42

Channel configuration set to: GFP: 11-Jul-2019 23:53:43

Channel:GFP set at:11-Jul-2019 23:53:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 11-Jul-2019 23:53:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:46

Channel:Brightfield set at:11-Jul-2019 23:53:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 23:53:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:47

Channel configuration set to: GFP: 11-Jul-2019 23:53:48

Channel:GFP set at:11-Jul-2019 23:53:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 11-Jul-2019 23:53:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:48

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:50

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:52

Channel:Brightfield set at:11-Jul-2019 23:53:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 23:53:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:52

Channel configuration set to: GFP: 11-Jul-2019 23:53:53

Channel:GFP set at:11-Jul-2019 23:53:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 11-Jul-2019 23:53:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:55

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.325

Channel configuration set to: Brightfield: 11-Jul-2019 23:53:57

Channel:Brightfield set at:11-Jul-2019 23:53:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4501. 11-Jul-2019 23:53:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:57

Channel configuration set to: GFP: 11-Jul-2019 23:53:58

Channel:GFP set at:11-Jul-2019 23:53:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4501. 11-Jul-2019 23:53:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:53:59

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:54:00

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 23:54:02

Channel:Brightfield set at:11-Jul-2019 23:54:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 23:54:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:54:02

Channel configuration set to: GFP: 11-Jul-2019 23:54:03

Channel:GFP set at:11-Jul-2019 23:54:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 11-Jul-2019 23:54:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:54:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:90 completed at:11-Jul-2019 23:54:05

Time since start of timelapse:26791.9247

PFS is locked

Time to next time point:208.6926



------Time point_91------

Memory available for all arrays: 61095374848

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:57:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 11-Jul-2019 23:57:36

Channel:Brightfield set at:11-Jul-2019 23:57:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 23:57:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:57:36

Channel configuration set to: GFP: 11-Jul-2019 23:57:37

Channel:GFP set at:11-Jul-2019 23:57:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 11-Jul-2019 23:57:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:57:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:57:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.3

Channel configuration set to: Brightfield: 11-Jul-2019 23:57:41

Channel:Brightfield set at:11-Jul-2019 23:57:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 23:57:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:57:41

Channel configuration set to: GFP: 11-Jul-2019 23:57:43

Channel:GFP set at:11-Jul-2019 23:57:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 11-Jul-2019 23:57:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:57:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:57:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 11-Jul-2019 23:57:46

Channel:Brightfield set at:11-Jul-2019 23:57:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 11-Jul-2019 23:57:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:57:46

Channel configuration set to: GFP: 11-Jul-2019 23:57:48

Channel:GFP set at:11-Jul-2019 23:57:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 11-Jul-2019 23:57:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:57:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:57:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.6

Channel configuration set to: Brightfield: 11-Jul-2019 23:57:51

Channel:Brightfield set at:11-Jul-2019 23:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 23:57:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:57:52

Channel configuration set to: GFP: 11-Jul-2019 23:57:53

Channel:GFP set at:11-Jul-2019 23:57:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 11-Jul-2019 23:57:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:57:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:57:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.7

Channel configuration set to: Brightfield: 11-Jul-2019 23:57:57

Channel:Brightfield set at:11-Jul-2019 23:57:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 23:57:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:57:57

Channel configuration set to: GFP: 11-Jul-2019 23:57:58

Channel:GFP set at:11-Jul-2019 23:57:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 11-Jul-2019 23:57:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:57:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:01

Channel:Brightfield set at:11-Jul-2019 23:58:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 11-Jul-2019 23:58:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:02

Channel configuration set to: GFP: 11-Jul-2019 23:58:03

Channel:GFP set at:11-Jul-2019 23:58:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 11-Jul-2019 23:58:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:07

Channel:Brightfield set at:11-Jul-2019 23:58:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 11-Jul-2019 23:58:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:07

Channel configuration set to: GFP: 11-Jul-2019 23:58:08

Channel:GFP set at:11-Jul-2019 23:58:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 11-Jul-2019 23:58:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.5

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:12

Channel:Brightfield set at:11-Jul-2019 23:58:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 23:58:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:13

Channel configuration set to: GFP: 11-Jul-2019 23:58:14

Channel:GFP set at:11-Jul-2019 23:58:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 11-Jul-2019 23:58:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:17

Channel:Brightfield set at:11-Jul-2019 23:58:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 11-Jul-2019 23:58:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:17

Channel configuration set to: GFP: 11-Jul-2019 23:58:19

Channel:GFP set at:11-Jul-2019 23:58:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 11-Jul-2019 23:58:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:22

Channel:Brightfield set at:11-Jul-2019 23:58:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 23:58:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:23

Channel configuration set to: GFP: 11-Jul-2019 23:58:24

Channel:GFP set at:11-Jul-2019 23:58:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 11-Jul-2019 23:58:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:28

Channel:Brightfield set at:11-Jul-2019 23:58:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 23:58:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:28

Channel configuration set to: GFP: 11-Jul-2019 23:58:30

Channel:GFP set at:11-Jul-2019 23:58:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 11-Jul-2019 23:58:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:32

Channel:Brightfield set at:11-Jul-2019 23:58:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 11-Jul-2019 23:58:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:33

Channel configuration set to: GFP: 11-Jul-2019 23:58:34

Channel:GFP set at:11-Jul-2019 23:58:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 11-Jul-2019 23:58:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:38

Channel:Brightfield set at:11-Jul-2019 23:58:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 23:58:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:38

Channel configuration set to: GFP: 11-Jul-2019 23:58:39

Channel:GFP set at:11-Jul-2019 23:58:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 11-Jul-2019 23:58:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:43

Channel:Brightfield set at:11-Jul-2019 23:58:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 23:58:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:43

Channel configuration set to: GFP: 11-Jul-2019 23:58:44

Channel:GFP set at:11-Jul-2019 23:58:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 11-Jul-2019 23:58:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:47

Channel:Brightfield set at:11-Jul-2019 23:58:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 23:58:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:48

Channel configuration set to: GFP: 11-Jul-2019 23:58:49

Channel:GFP set at:11-Jul-2019 23:58:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 11-Jul-2019 23:58:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:53

Channel:Brightfield set at:11-Jul-2019 23:58:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 11-Jul-2019 23:58:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:53

Channel configuration set to: GFP: 11-Jul-2019 23:58:54

Channel:GFP set at:11-Jul-2019 23:58:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6751. 11-Jul-2019 23:58:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.35

Channel configuration set to: Brightfield: 11-Jul-2019 23:58:58

Channel:Brightfield set at:11-Jul-2019 23:58:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 23:58:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:58:58

Channel configuration set to: GFP: 11-Jul-2019 23:58:59

Channel:GFP set at:11-Jul-2019 23:58:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 11-Jul-2019 23:59:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:59:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 11-Jul-2019 23:59:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 11-Jul-2019 23:59:03

Channel:Brightfield set at:11-Jul-2019 23:59:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 23:59:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:59:03

Channel configuration set to: GFP: 11-Jul-2019 23:59:04

Channel:GFP set at:11-Jul-2019 23:59:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 11-Jul-2019 23:59:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 11-Jul-2019 23:59:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:91 completed at:11-Jul-2019 23:59:07

Time since start of timelapse:27093.1173

PFS is locked

Time to next time point:207.5017



------Time point_92------

Memory available for all arrays: 61098082304

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:02:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.1

Channel configuration set to: Brightfield: 12-Jul-2019 00:02:36

Channel:Brightfield set at:12-Jul-2019 00:02:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1751. 12-Jul-2019 00:02:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:02:36

Channel configuration set to: GFP: 12-Jul-2019 00:02:37

Channel:GFP set at:12-Jul-2019 00:02:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2001. 12-Jul-2019 00:02:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:02:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:02:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 12-Jul-2019 00:02:41

Channel:Brightfield set at:12-Jul-2019 00:02:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 00:02:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:02:41

Channel configuration set to: GFP: 12-Jul-2019 00:02:42

Channel:GFP set at:12-Jul-2019 00:02:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6751. 12-Jul-2019 00:02:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:02:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:02:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.875

Channel configuration set to: Brightfield: 12-Jul-2019 00:02:46

Channel:Brightfield set at:12-Jul-2019 00:02:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0751. 12-Jul-2019 00:02:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:02:46

Channel configuration set to: GFP: 12-Jul-2019 00:02:47

Channel:GFP set at:12-Jul-2019 00:02:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 12-Jul-2019 00:02:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:02:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:02:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.375

Channel configuration set to: Brightfield: 12-Jul-2019 00:02:51

Channel:Brightfield set at:12-Jul-2019 00:02:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 00:02:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:02:51

Channel configuration set to: GFP: 12-Jul-2019 00:02:53

Channel:GFP set at:12-Jul-2019 00:02:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 00:02:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:02:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:02:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 12-Jul-2019 00:02:56

Channel:Brightfield set at:12-Jul-2019 00:02:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 00:02:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:02:57

Channel configuration set to: GFP: 12-Jul-2019 00:02:58

Channel:GFP set at:12-Jul-2019 00:02:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 00:02:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:02:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:02

Channel:Brightfield set at:12-Jul-2019 00:03:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 00:03:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:02

Channel configuration set to: GFP: 12-Jul-2019 00:03:03

Channel:GFP set at:12-Jul-2019 00:03:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 00:03:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:06

Channel:Brightfield set at:12-Jul-2019 00:03:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 00:03:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:07

Channel configuration set to: GFP: 12-Jul-2019 00:03:08

Channel:GFP set at:12-Jul-2019 00:03:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 00:03:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:12

Channel:Brightfield set at:12-Jul-2019 00:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 12-Jul-2019 00:03:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:13

Channel configuration set to: GFP: 12-Jul-2019 00:03:14

Channel:GFP set at:12-Jul-2019 00:03:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 12-Jul-2019 00:03:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:17

Channel:Brightfield set at:12-Jul-2019 00:03:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 00:03:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:18

Channel configuration set to: GFP: 12-Jul-2019 00:03:19

Channel:GFP set at:12-Jul-2019 00:03:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 00:03:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:22

Channel:Brightfield set at:12-Jul-2019 00:03:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 00:03:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:23

Channel configuration set to: GFP: 12-Jul-2019 00:03:24

Channel:GFP set at:12-Jul-2019 00:03:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 00:03:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:27

Channel:Brightfield set at:12-Jul-2019 00:03:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 00:03:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:28

Channel configuration set to: GFP: 12-Jul-2019 00:03:29

Channel:GFP set at:12-Jul-2019 00:03:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 00:03:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:32

Channel:Brightfield set at:12-Jul-2019 00:03:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 00:03:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:33

Channel configuration set to: GFP: 12-Jul-2019 00:03:34

Channel:GFP set at:12-Jul-2019 00:03:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 00:03:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:38

Channel:Brightfield set at:12-Jul-2019 00:03:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 00:03:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:38

Channel configuration set to: GFP: 12-Jul-2019 00:03:40

Channel:GFP set at:12-Jul-2019 00:03:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 00:03:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:43

Channel:Brightfield set at:12-Jul-2019 00:03:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 00:03:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:43

Channel configuration set to: GFP: 12-Jul-2019 00:03:45

Channel:GFP set at:12-Jul-2019 00:03:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 00:03:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:48

Channel:Brightfield set at:12-Jul-2019 00:03:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 00:03:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:48

Channel configuration set to: GFP: 12-Jul-2019 00:03:49

Channel:GFP set at:12-Jul-2019 00:03:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 00:03:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.525

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:53

Channel:Brightfield set at:12-Jul-2019 00:03:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 00:03:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:54

Channel configuration set to: GFP: 12-Jul-2019 00:03:55

Channel:GFP set at:12-Jul-2019 00:03:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 00:03:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 12-Jul-2019 00:03:58

Channel:Brightfield set at:12-Jul-2019 00:03:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 00:03:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:03:58

Channel configuration set to: GFP: 12-Jul-2019 00:03:59

Channel:GFP set at:12-Jul-2019 00:03:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 00:04:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:04:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:04:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 12-Jul-2019 00:04:03

Channel:Brightfield set at:12-Jul-2019 00:04:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 00:04:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:04:03

Channel configuration set to: GFP: 12-Jul-2019 00:04:04

Channel:GFP set at:12-Jul-2019 00:04:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 00:04:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:04:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:92 completed at:12-Jul-2019 00:04:07

Time since start of timelapse:27393.1029

PFS is locked

Time to next time point:207.5174



------Time point_93------

Memory available for all arrays: 61095948288

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:07:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.975

Channel configuration set to: Brightfield: 12-Jul-2019 00:07:36

Channel:Brightfield set at:12-Jul-2019 00:07:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3001. 12-Jul-2019 00:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:07:36

Channel configuration set to: GFP: 12-Jul-2019 00:07:37

Channel:GFP set at:12-Jul-2019 00:07:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 00:07:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:07:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:07:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.375

Channel configuration set to: Brightfield: 12-Jul-2019 00:07:40

Channel:Brightfield set at:12-Jul-2019 00:07:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 12-Jul-2019 00:07:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:07:41

Channel configuration set to: GFP: 12-Jul-2019 00:07:42

Channel:GFP set at:12-Jul-2019 00:07:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 12-Jul-2019 00:07:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:07:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:07:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.7

Channel configuration set to: Brightfield: 12-Jul-2019 00:07:46

Channel:Brightfield set at:12-Jul-2019 00:07:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 12-Jul-2019 00:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:07:46

Channel configuration set to: GFP: 12-Jul-2019 00:07:47

Channel:GFP set at:12-Jul-2019 00:07:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 12-Jul-2019 00:07:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:07:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:07:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 12-Jul-2019 00:07:51

Channel:Brightfield set at:12-Jul-2019 00:07:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 00:07:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:07:51

Channel configuration set to: GFP: 12-Jul-2019 00:07:53

Channel:GFP set at:12-Jul-2019 00:07:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 00:07:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:07:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:07:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.35

Channel configuration set to: Brightfield: 12-Jul-2019 00:07:56

Channel:Brightfield set at:12-Jul-2019 00:07:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 00:07:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:07:57

Channel configuration set to: GFP: 12-Jul-2019 00:07:58

Channel:GFP set at:12-Jul-2019 00:07:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 00:07:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:07:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:02

Channel:Brightfield set at:12-Jul-2019 00:08:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 00:08:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:02

Channel configuration set to: GFP: 12-Jul-2019 00:08:03

Channel:GFP set at:12-Jul-2019 00:08:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 00:08:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:06

Channel:Brightfield set at:12-Jul-2019 00:08:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 00:08:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:07

Channel configuration set to: GFP: 12-Jul-2019 00:08:08

Channel:GFP set at:12-Jul-2019 00:08:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 00:08:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:11

Channel:Brightfield set at:12-Jul-2019 00:08:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 00:08:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:12

Channel configuration set to: GFP: 12-Jul-2019 00:08:13

Channel:GFP set at:12-Jul-2019 00:08:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 00:08:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.625

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:16

Channel:Brightfield set at:12-Jul-2019 00:08:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 00:08:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:16

Channel configuration set to: GFP: 12-Jul-2019 00:08:18

Channel:GFP set at:12-Jul-2019 00:08:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 00:08:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:22

Channel:Brightfield set at:12-Jul-2019 00:08:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9251. 12-Jul-2019 00:08:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:22

Channel configuration set to: GFP: 12-Jul-2019 00:08:23

Channel:GFP set at:12-Jul-2019 00:08:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9251. 12-Jul-2019 00:08:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:27

Channel:Brightfield set at:12-Jul-2019 00:08:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 00:08:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:27

Channel configuration set to: GFP: 12-Jul-2019 00:08:28

Channel:GFP set at:12-Jul-2019 00:08:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 00:08:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:31

Channel:Brightfield set at:12-Jul-2019 00:08:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 00:08:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:32

Channel configuration set to: GFP: 12-Jul-2019 00:08:33

Channel:GFP set at:12-Jul-2019 00:08:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 00:08:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:33

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:35

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:37

Channel:Brightfield set at:12-Jul-2019 00:08:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 00:08:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:38

Channel configuration set to: GFP: 12-Jul-2019 00:08:39

Channel:GFP set at:12-Jul-2019 00:08:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 00:08:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:43

Channel:Brightfield set at:12-Jul-2019 00:08:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 00:08:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:43

Channel configuration set to: GFP: 12-Jul-2019 00:08:44

Channel:GFP set at:12-Jul-2019 00:08:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 00:08:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:47

Channel:Brightfield set at:12-Jul-2019 00:08:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 00:08:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:48

Channel configuration set to: GFP: 12-Jul-2019 00:08:49

Channel:GFP set at:12-Jul-2019 00:08:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 00:08:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:52

Channel:Brightfield set at:12-Jul-2019 00:08:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3251. 12-Jul-2019 00:08:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:52

Channel configuration set to: GFP: 12-Jul-2019 00:08:54

Channel:GFP set at:12-Jul-2019 00:08:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 12-Jul-2019 00:08:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.075

Channel configuration set to: Brightfield: 12-Jul-2019 00:08:57

Channel:Brightfield set at:12-Jul-2019 00:08:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 12-Jul-2019 00:08:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:08:58

Channel configuration set to: GFP: 12-Jul-2019 00:08:59

Channel:GFP set at:12-Jul-2019 00:08:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 12-Jul-2019 00:09:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:09:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:09:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 12-Jul-2019 00:09:02

Channel:Brightfield set at:12-Jul-2019 00:09:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 12-Jul-2019 00:09:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:09:03

Channel configuration set to: GFP: 12-Jul-2019 00:09:04

Channel:GFP set at:12-Jul-2019 00:09:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 12-Jul-2019 00:09:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:09:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:93 completed at:12-Jul-2019 00:09:06

Time since start of timelapse:27692.9459

PFS is locked

Time to next time point:207.6756



------Time point_94------

Memory available for all arrays: 61090852864

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:12:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.95

Channel configuration set to: Brightfield: 12-Jul-2019 00:12:36

Channel:Brightfield set at:12-Jul-2019 00:12:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 00:12:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:12:36

Channel configuration set to: GFP: 12-Jul-2019 00:12:37

Channel:GFP set at:12-Jul-2019 00:12:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 00:12:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:12:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:12:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.8

Channel configuration set to: Brightfield: 12-Jul-2019 00:12:41

Channel:Brightfield set at:12-Jul-2019 00:12:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 12-Jul-2019 00:12:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:12:41

Channel configuration set to: GFP: 12-Jul-2019 00:12:43

Channel:GFP set at:12-Jul-2019 00:12:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 12-Jul-2019 00:12:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:12:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:12:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.775

Channel configuration set to: Brightfield: 12-Jul-2019 00:12:46

Channel:Brightfield set at:12-Jul-2019 00:12:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1001. 12-Jul-2019 00:12:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:12:46

Channel configuration set to: GFP: 12-Jul-2019 00:12:48

Channel:GFP set at:12-Jul-2019 00:12:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.1001. 12-Jul-2019 00:12:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:12:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:12:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 12-Jul-2019 00:12:51

Channel:Brightfield set at:12-Jul-2019 00:12:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 00:12:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:12:51

Channel configuration set to: GFP: 12-Jul-2019 00:12:53

Channel:GFP set at:12-Jul-2019 00:12:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 12-Jul-2019 00:12:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:12:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:12:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 12-Jul-2019 00:12:56

Channel:Brightfield set at:12-Jul-2019 00:12:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 00:12:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:12:57

Channel configuration set to: GFP: 12-Jul-2019 00:12:58

Channel:GFP set at:12-Jul-2019 00:12:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 00:12:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:12:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:02

Channel:Brightfield set at:12-Jul-2019 00:13:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 00:13:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:02

Channel configuration set to: GFP: 12-Jul-2019 00:13:04

Channel:GFP set at:12-Jul-2019 00:13:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 00:13:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:07

Channel:Brightfield set at:12-Jul-2019 00:13:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 00:13:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:07

Channel configuration set to: GFP: 12-Jul-2019 00:13:08

Channel:GFP set at:12-Jul-2019 00:13:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 00:13:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:12

Channel:Brightfield set at:12-Jul-2019 00:13:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 00:13:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:13

Channel configuration set to: GFP: 12-Jul-2019 00:13:14

Channel:GFP set at:12-Jul-2019 00:13:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 00:13:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:18

Channel:Brightfield set at:12-Jul-2019 00:13:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 00:13:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:18

Channel configuration set to: GFP: 12-Jul-2019 00:13:20

Channel:GFP set at:12-Jul-2019 00:13:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 00:13:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:23

Channel:Brightfield set at:12-Jul-2019 00:13:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 12-Jul-2019 00:13:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:23

Channel configuration set to: GFP: 12-Jul-2019 00:13:24

Channel:GFP set at:12-Jul-2019 00:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 12-Jul-2019 00:13:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:28

Channel:Brightfield set at:12-Jul-2019 00:13:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 00:13:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:28

Channel configuration set to: GFP: 12-Jul-2019 00:13:29

Channel:GFP set at:12-Jul-2019 00:13:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 00:13:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:32

Channel:Brightfield set at:12-Jul-2019 00:13:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 00:13:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:33

Channel configuration set to: GFP: 12-Jul-2019 00:13:34

Channel:GFP set at:12-Jul-2019 00:13:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 00:13:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:38

Channel:Brightfield set at:12-Jul-2019 00:13:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 00:13:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:39

Channel configuration set to: GFP: 12-Jul-2019 00:13:40

Channel:GFP set at:12-Jul-2019 00:13:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 00:13:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:44

Channel:Brightfield set at:12-Jul-2019 00:13:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 00:13:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:44

Channel configuration set to: GFP: 12-Jul-2019 00:13:45

Channel:GFP set at:12-Jul-2019 00:13:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 00:13:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:48

Channel:Brightfield set at:12-Jul-2019 00:13:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 00:13:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:49

Channel configuration set to: GFP: 12-Jul-2019 00:13:50

Channel:GFP set at:12-Jul-2019 00:13:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 00:13:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.325

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:54

Channel:Brightfield set at:12-Jul-2019 00:13:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 00:13:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:54

Channel configuration set to: GFP: 12-Jul-2019 00:13:55

Channel:GFP set at:12-Jul-2019 00:13:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 00:13:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 12-Jul-2019 00:13:58

Channel:Brightfield set at:12-Jul-2019 00:13:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 12-Jul-2019 00:13:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:13:58

Channel configuration set to: GFP: 12-Jul-2019 00:14:00

Channel:GFP set at:12-Jul-2019 00:14:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 12-Jul-2019 00:14:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:14:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:14:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 12-Jul-2019 00:14:04

Channel:Brightfield set at:12-Jul-2019 00:14:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 12-Jul-2019 00:14:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:14:04

Channel configuration set to: GFP: 12-Jul-2019 00:14:05

Channel:GFP set at:12-Jul-2019 00:14:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 12-Jul-2019 00:14:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:14:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:94 completed at:12-Jul-2019 00:14:07

Time since start of timelapse:27993.9927

PFS is locked

Time to next time point:206.63



------Time point_95------

Memory available for all arrays: 61096673280

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:17:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.875

Channel configuration set to: Brightfield: 12-Jul-2019 00:17:36

Channel:Brightfield set at:12-Jul-2019 00:17:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 00:17:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:17:36

Channel configuration set to: GFP: 12-Jul-2019 00:17:37

Channel:GFP set at:12-Jul-2019 00:17:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 00:17:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:17:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:17:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.8

Channel configuration set to: Brightfield: 12-Jul-2019 00:17:41

Channel:Brightfield set at:12-Jul-2019 00:17:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0751. 12-Jul-2019 00:17:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:17:41

Channel configuration set to: GFP: 12-Jul-2019 00:17:43

Channel:GFP set at:12-Jul-2019 00:17:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 12-Jul-2019 00:17:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:17:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:17:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 12-Jul-2019 00:17:45

Channel:Brightfield set at:12-Jul-2019 00:17:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2001. 12-Jul-2019 00:17:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:17:46

Channel configuration set to: GFP: 12-Jul-2019 00:17:47

Channel:GFP set at:12-Jul-2019 00:17:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.2251. 12-Jul-2019 00:17:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:17:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:17:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 00:17:51

Channel:Brightfield set at:12-Jul-2019 00:17:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 00:17:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:17:51

Channel configuration set to: GFP: 12-Jul-2019 00:17:52

Channel:GFP set at:12-Jul-2019 00:17:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 00:17:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:17:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:17:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 12-Jul-2019 00:17:56

Channel:Brightfield set at:12-Jul-2019 00:17:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 00:17:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:17:56

Channel configuration set to: GFP: 12-Jul-2019 00:17:58

Channel:GFP set at:12-Jul-2019 00:17:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 00:17:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:17:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.925

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:01

Channel:Brightfield set at:12-Jul-2019 00:18:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2251. 12-Jul-2019 00:18:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:01

Channel configuration set to: GFP: 12-Jul-2019 00:18:03

Channel:GFP set at:12-Jul-2019 00:18:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2251. 12-Jul-2019 00:18:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:07

Channel:Brightfield set at:12-Jul-2019 00:18:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 00:18:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:07

Channel configuration set to: GFP: 12-Jul-2019 00:18:08

Channel:GFP set at:12-Jul-2019 00:18:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 00:18:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:13

Channel:Brightfield set at:12-Jul-2019 00:18:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 00:18:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:13

Channel configuration set to: GFP: 12-Jul-2019 00:18:15

Channel:GFP set at:12-Jul-2019 00:18:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 00:18:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:17

Channel:Brightfield set at:12-Jul-2019 00:18:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 00:18:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:18

Channel configuration set to: GFP: 12-Jul-2019 00:18:19

Channel:GFP set at:12-Jul-2019 00:18:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0001. 12-Jul-2019 00:18:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:22

Channel:Brightfield set at:12-Jul-2019 00:18:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 00:18:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:23

Channel configuration set to: GFP: 12-Jul-2019 00:18:24

Channel:GFP set at:12-Jul-2019 00:18:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 00:18:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:28

Channel:Brightfield set at:12-Jul-2019 00:18:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 00:18:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:28

Channel configuration set to: GFP: 12-Jul-2019 00:18:29

Channel:GFP set at:12-Jul-2019 00:18:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 00:18:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:32

Channel:Brightfield set at:12-Jul-2019 00:18:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2251. 12-Jul-2019 00:18:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:33

Channel configuration set to: GFP: 12-Jul-2019 00:18:34

Channel:GFP set at:12-Jul-2019 00:18:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 00:18:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:38

Channel:Brightfield set at:12-Jul-2019 00:18:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 00:18:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:39

Channel configuration set to: GFP: 12-Jul-2019 00:18:40

Channel:GFP set at:12-Jul-2019 00:18:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 00:18:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:44

Channel:Brightfield set at:12-Jul-2019 00:18:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 00:18:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:44

Channel configuration set to: GFP: 12-Jul-2019 00:18:45

Channel:GFP set at:12-Jul-2019 00:18:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 00:18:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:49

Channel:Brightfield set at:12-Jul-2019 00:18:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 00:18:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:49

Channel configuration set to: GFP: 12-Jul-2019 00:18:51

Channel:GFP set at:12-Jul-2019 00:18:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 00:18:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.35

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:54

Channel:Brightfield set at:12-Jul-2019 00:18:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 00:18:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:55

Channel configuration set to: GFP: 12-Jul-2019 00:18:56

Channel:GFP set at:12-Jul-2019 00:18:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 12-Jul-2019 00:18:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:18:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.975

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 00:18:59

Channel:Brightfield set at:12-Jul-2019 00:18:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 12-Jul-2019 00:19:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:19:00

Channel configuration set to: GFP: 12-Jul-2019 00:19:01

Channel:GFP set at:12-Jul-2019 00:19:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 12-Jul-2019 00:19:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:19:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:19:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 12-Jul-2019 00:19:04

Channel:Brightfield set at:12-Jul-2019 00:19:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 12-Jul-2019 00:19:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:19:05

Channel configuration set to: GFP: 12-Jul-2019 00:19:06

Channel:GFP set at:12-Jul-2019 00:19:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 12-Jul-2019 00:19:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:19:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:95 completed at:12-Jul-2019 00:19:08

Time since start of timelapse:28294.9315

PFS is locked

Time to next time point:205.6924



------Time point_96------

Memory available for all arrays: 61095661568

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:22:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.8

Channel configuration set to: Brightfield: 12-Jul-2019 00:22:36

Channel:Brightfield set at:12-Jul-2019 00:22:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 00:22:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:22:36

Channel configuration set to: GFP: 12-Jul-2019 00:22:37

Channel:GFP set at:12-Jul-2019 00:22:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 00:22:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:22:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:22:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.85

Channel configuration set to: Brightfield: 12-Jul-2019 00:22:41

Channel:Brightfield set at:12-Jul-2019 00:22:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 00:22:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:22:41

Channel configuration set to: GFP: 12-Jul-2019 00:22:43

Channel:GFP set at:12-Jul-2019 00:22:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 00:22:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:22:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:22:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.95

Channel configuration set to: Brightfield: 12-Jul-2019 00:22:46

Channel:Brightfield set at:12-Jul-2019 00:22:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 12-Jul-2019 00:22:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:22:46

Channel configuration set to: GFP: 12-Jul-2019 00:22:48

Channel:GFP set at:12-Jul-2019 00:22:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 12-Jul-2019 00:22:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:22:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:22:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.075

Channel configuration set to: Brightfield: 12-Jul-2019 00:22:51

Channel:Brightfield set at:12-Jul-2019 00:22:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 12-Jul-2019 00:22:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:22:52

Channel configuration set to: GFP: 12-Jul-2019 00:22:53

Channel:GFP set at:12-Jul-2019 00:22:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 12-Jul-2019 00:22:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:22:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:22:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 12-Jul-2019 00:22:56

Channel:Brightfield set at:12-Jul-2019 00:22:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 00:22:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:22:56

Channel configuration set to: GFP: 12-Jul-2019 00:22:58

Channel:GFP set at:12-Jul-2019 00:22:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 00:22:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:22:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.925

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:01

Channel:Brightfield set at:12-Jul-2019 00:23:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 00:23:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:02

Channel configuration set to: GFP: 12-Jul-2019 00:23:03

Channel:GFP set at:12-Jul-2019 00:23:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 00:23:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:06

Channel:Brightfield set at:12-Jul-2019 00:23:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 00:23:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:07

Channel configuration set to: GFP: 12-Jul-2019 00:23:08

Channel:GFP set at:12-Jul-2019 00:23:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 00:23:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:12

Channel:Brightfield set at:12-Jul-2019 00:23:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 00:23:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:13

Channel configuration set to: GFP: 12-Jul-2019 00:23:14

Channel:GFP set at:12-Jul-2019 00:23:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 00:23:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.725

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:18

Channel:Brightfield set at:12-Jul-2019 00:23:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 00:23:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:18

Channel configuration set to: GFP: 12-Jul-2019 00:23:19

Channel:GFP set at:12-Jul-2019 00:23:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 00:23:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:22

Channel:Brightfield set at:12-Jul-2019 00:23:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 00:23:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:23

Channel configuration set to: GFP: 12-Jul-2019 00:23:24

Channel:GFP set at:12-Jul-2019 00:23:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0001. 12-Jul-2019 00:23:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:27

Channel:Brightfield set at:12-Jul-2019 00:23:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 00:23:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:28

Channel configuration set to: GFP: 12-Jul-2019 00:23:29

Channel:GFP set at:12-Jul-2019 00:23:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 00:23:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:33

Channel:Brightfield set at:12-Jul-2019 00:23:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 00:23:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:33

Channel configuration set to: GFP: 12-Jul-2019 00:23:35

Channel:GFP set at:12-Jul-2019 00:23:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 00:23:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:38

Channel:Brightfield set at:12-Jul-2019 00:23:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 00:23:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:38

Channel configuration set to: GFP: 12-Jul-2019 00:23:39

Channel:GFP set at:12-Jul-2019 00:23:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 00:23:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:43

Channel:Brightfield set at:12-Jul-2019 00:23:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 00:23:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:43

Channel configuration set to: GFP: 12-Jul-2019 00:23:44

Channel:GFP set at:12-Jul-2019 00:23:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 00:23:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.4

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:48

Channel:Brightfield set at:12-Jul-2019 00:23:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 00:23:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:48

Channel configuration set to: GFP: 12-Jul-2019 00:23:50

Channel:GFP set at:12-Jul-2019 00:23:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 00:23:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.225

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:53

Channel:Brightfield set at:12-Jul-2019 00:23:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 12-Jul-2019 00:23:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:54

Channel configuration set to: GFP: 12-Jul-2019 00:23:55

Channel:GFP set at:12-Jul-2019 00:23:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 12-Jul-2019 00:23:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.75

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 00:23:58

Channel:Brightfield set at:12-Jul-2019 00:23:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 12-Jul-2019 00:23:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:23:59

Channel configuration set to: GFP: 12-Jul-2019 00:24:00

Channel:GFP set at:12-Jul-2019 00:24:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 12-Jul-2019 00:24:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:24:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:24:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 00:24:03

Channel:Brightfield set at:12-Jul-2019 00:24:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8001. 12-Jul-2019 00:24:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:24:04

Channel configuration set to: GFP: 12-Jul-2019 00:24:05

Channel:GFP set at:12-Jul-2019 00:24:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7751. 12-Jul-2019 00:24:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:24:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:96 completed at:12-Jul-2019 00:24:07

Time since start of timelapse:28593.7909

PFS is locked

Time to next time point:206.8346



------Time point_97------

Memory available for all arrays: 61096247296

Memory used by Matlab: 2209243136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:27:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-11.225

Channel configuration set to: Brightfield: 12-Jul-2019 00:27:35

Channel:Brightfield set at:12-Jul-2019 00:27:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4501. 12-Jul-2019 00:27:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:27:36

Channel configuration set to: GFP: 12-Jul-2019 00:27:37

Channel:GFP set at:12-Jul-2019 00:27:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4251. 12-Jul-2019 00:27:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:27:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:27:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.775

Channel configuration set to: Brightfield: 12-Jul-2019 00:27:41

Channel:Brightfield set at:12-Jul-2019 00:27:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 00:27:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:27:41

Channel configuration set to: GFP: 12-Jul-2019 00:27:42

Channel:GFP set at:12-Jul-2019 00:27:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 00:27:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:27:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:27:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.575

Channel configuration set to: Brightfield: 12-Jul-2019 00:27:46

Channel:Brightfield set at:12-Jul-2019 00:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 00:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:27:46

Channel configuration set to: GFP: 12-Jul-2019 00:27:48

Channel:GFP set at:12-Jul-2019 00:27:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 00:27:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:27:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:27:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.025

Channel configuration set to: Brightfield: 12-Jul-2019 00:27:51

Channel:Brightfield set at:12-Jul-2019 00:27:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 12-Jul-2019 00:27:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:27:51

Channel configuration set to: GFP: 12-Jul-2019 00:27:53

Channel:GFP set at:12-Jul-2019 00:27:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 12-Jul-2019 00:27:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:27:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:27:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.35

Channel configuration set to: Brightfield: 12-Jul-2019 00:27:57

Channel:Brightfield set at:12-Jul-2019 00:27:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 00:27:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:27:57

Channel configuration set to: GFP: 12-Jul-2019 00:27:58

Channel:GFP set at:12-Jul-2019 00:27:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 00:27:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:27:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.525

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:01

Channel:Brightfield set at:12-Jul-2019 00:28:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1001. 12-Jul-2019 00:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:02

Channel configuration set to: GFP: 12-Jul-2019 00:28:03

Channel:GFP set at:12-Jul-2019 00:28:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1001. 12-Jul-2019 00:28:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:06

Channel:Brightfield set at:12-Jul-2019 00:28:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 00:28:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:07

Channel configuration set to: GFP: 12-Jul-2019 00:28:08

Channel:GFP set at:12-Jul-2019 00:28:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 00:28:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:12

Channel:Brightfield set at:12-Jul-2019 00:28:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 00:28:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:13

Channel configuration set to: GFP: 12-Jul-2019 00:28:14

Channel:GFP set at:12-Jul-2019 00:28:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 00:28:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:17

Channel:Brightfield set at:12-Jul-2019 00:28:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 00:28:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:17

Channel configuration set to: GFP: 12-Jul-2019 00:28:19

Channel:GFP set at:12-Jul-2019 00:28:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 00:28:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:22

Channel:Brightfield set at:12-Jul-2019 00:28:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 00:28:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:23

Channel configuration set to: GFP: 12-Jul-2019 00:28:24

Channel:GFP set at:12-Jul-2019 00:28:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 00:28:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:27

Channel:Brightfield set at:12-Jul-2019 00:28:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 00:28:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:27

Channel configuration set to: GFP: 12-Jul-2019 00:28:29

Channel:GFP set at:12-Jul-2019 00:28:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 00:28:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:32

Channel:Brightfield set at:12-Jul-2019 00:28:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 12-Jul-2019 00:28:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:33

Channel configuration set to: GFP: 12-Jul-2019 00:28:34

Channel:GFP set at:12-Jul-2019 00:28:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 12-Jul-2019 00:28:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:38

Channel:Brightfield set at:12-Jul-2019 00:28:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 00:28:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:38

Channel configuration set to: GFP: 12-Jul-2019 00:28:40

Channel:GFP set at:12-Jul-2019 00:28:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 00:28:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:43

Channel:Brightfield set at:12-Jul-2019 00:28:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 00:28:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:44

Channel configuration set to: GFP: 12-Jul-2019 00:28:45

Channel:GFP set at:12-Jul-2019 00:28:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 00:28:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:48

Channel:Brightfield set at:12-Jul-2019 00:28:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 00:28:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:48

Channel configuration set to: GFP: 12-Jul-2019 00:28:50

Channel:GFP set at:12-Jul-2019 00:28:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 00:28:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.2

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:53

Channel:Brightfield set at:12-Jul-2019 00:28:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 12-Jul-2019 00:28:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:54

Channel configuration set to: GFP: 12-Jul-2019 00:28:55

Channel:GFP set at:12-Jul-2019 00:28:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1751. 12-Jul-2019 00:28:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5

Channel configuration set to: Brightfield: 12-Jul-2019 00:28:58

Channel:Brightfield set at:12-Jul-2019 00:28:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 12-Jul-2019 00:28:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:28:59

Channel configuration set to: GFP: 12-Jul-2019 00:29:00

Channel:GFP set at:12-Jul-2019 00:29:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 12-Jul-2019 00:29:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:29:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:29:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-4.625

Channel configuration set to: Brightfield: 12-Jul-2019 00:29:03

Channel:Brightfield set at:12-Jul-2019 00:29:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 00:29:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:29:03

Channel configuration set to: GFP: 12-Jul-2019 00:29:04

Channel:GFP set at:12-Jul-2019 00:29:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 00:29:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:29:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:97 completed at:12-Jul-2019 00:29:07

Time since start of timelapse:28893.5233

PFS is locked

Time to next time point:207.1034



------Time point_98------

Memory available for all arrays: 61090615296

Memory used by Matlab: 2209550336

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:32:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.025

Channel configuration set to: Brightfield: 12-Jul-2019 00:32:36

Channel:Brightfield set at:12-Jul-2019 00:32:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 12-Jul-2019 00:32:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:32:36

Channel configuration set to: GFP: 12-Jul-2019 00:32:37

Channel:GFP set at:12-Jul-2019 00:32:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 12-Jul-2019 00:32:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:32:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:32:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.025

Channel configuration set to: Brightfield: 12-Jul-2019 00:32:41

Channel:Brightfield set at:12-Jul-2019 00:32:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 00:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:32:41

Channel configuration set to: GFP: 12-Jul-2019 00:32:43

Channel:GFP set at:12-Jul-2019 00:32:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 00:32:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:32:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:32:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 12-Jul-2019 00:32:46

Channel:Brightfield set at:12-Jul-2019 00:32:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 12-Jul-2019 00:32:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:32:46

Channel configuration set to: GFP: 12-Jul-2019 00:32:47

Channel:GFP set at:12-Jul-2019 00:32:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 12-Jul-2019 00:32:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:32:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:32:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.375

Channel configuration set to: Brightfield: 12-Jul-2019 00:32:51

Channel:Brightfield set at:12-Jul-2019 00:32:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 12-Jul-2019 00:32:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:32:52

Channel configuration set to: GFP: 12-Jul-2019 00:32:53

Channel:GFP set at:12-Jul-2019 00:32:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 00:32:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:32:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:32:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 12-Jul-2019 00:32:56

Channel:Brightfield set at:12-Jul-2019 00:32:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 00:32:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:32:56

Channel configuration set to: GFP: 12-Jul-2019 00:32:58

Channel:GFP set at:12-Jul-2019 00:32:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 00:32:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:32:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:01

Channel:Brightfield set at:12-Jul-2019 00:33:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 00:33:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:01

Channel configuration set to: GFP: 12-Jul-2019 00:33:02

Channel:GFP set at:12-Jul-2019 00:33:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 00:33:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:06

Channel:Brightfield set at:12-Jul-2019 00:33:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 00:33:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:06

Channel configuration set to: GFP: 12-Jul-2019 00:33:08

Channel:GFP set at:12-Jul-2019 00:33:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 00:33:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:12

Channel:Brightfield set at:12-Jul-2019 00:33:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 00:33:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:12

Channel configuration set to: GFP: 12-Jul-2019 00:33:13

Channel:GFP set at:12-Jul-2019 00:33:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 00:33:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:17

Channel:Brightfield set at:12-Jul-2019 00:33:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 00:33:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:18

Channel configuration set to: GFP: 12-Jul-2019 00:33:19

Channel:GFP set at:12-Jul-2019 00:33:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 00:33:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:22

Channel:Brightfield set at:12-Jul-2019 00:33:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 00:33:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:22

Channel configuration set to: GFP: 12-Jul-2019 00:33:24

Channel:GFP set at:12-Jul-2019 00:33:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 00:33:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:27

Channel:Brightfield set at:12-Jul-2019 00:33:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 00:33:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:28

Channel configuration set to: GFP: 12-Jul-2019 00:33:29

Channel:GFP set at:12-Jul-2019 00:33:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 00:33:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:33

Channel:Brightfield set at:12-Jul-2019 00:33:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 12-Jul-2019 00:33:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:33

Channel configuration set to: GFP: 12-Jul-2019 00:33:34

Channel:GFP set at:12-Jul-2019 00:33:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 12-Jul-2019 00:33:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:38

Channel:Brightfield set at:12-Jul-2019 00:33:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 00:33:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:39

Channel configuration set to: GFP: 12-Jul-2019 00:33:40

Channel:GFP set at:12-Jul-2019 00:33:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 00:33:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:44

Channel:Brightfield set at:12-Jul-2019 00:33:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 00:33:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:44

Channel configuration set to: GFP: 12-Jul-2019 00:33:45

Channel:GFP set at:12-Jul-2019 00:33:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 00:33:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:48

Channel:Brightfield set at:12-Jul-2019 00:33:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 00:33:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:49

Channel configuration set to: GFP: 12-Jul-2019 00:33:50

Channel:GFP set at:12-Jul-2019 00:33:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 00:33:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.4

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:54

Channel:Brightfield set at:12-Jul-2019 00:33:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 00:33:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:54

Channel configuration set to: GFP: 12-Jul-2019 00:33:56

Channel:GFP set at:12-Jul-2019 00:33:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 00:33:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:33:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.775

PFS not locked. Waiting for lock...

PFS took 0.400000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 00:33:59

Channel:Brightfield set at:12-Jul-2019 00:33:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 00:34:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:34:00

Channel configuration set to: GFP: 12-Jul-2019 00:34:01

Channel:GFP set at:12-Jul-2019 00:34:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 00:34:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:34:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:34:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 00:34:04

Channel:Brightfield set at:12-Jul-2019 00:34:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 00:34:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:34:05

Channel configuration set to: GFP: 12-Jul-2019 00:34:06

Channel:GFP set at:12-Jul-2019 00:34:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 00:34:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:34:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:98 completed at:12-Jul-2019 00:34:08

Time since start of timelapse:29194.8275

PFS is locked

Time to next time point:205.8009



------Time point_99------

Memory available for all arrays: 61086162944

Memory used by Matlab: 2209857536

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:37:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 12-Jul-2019 00:37:36

Channel:Brightfield set at:12-Jul-2019 00:37:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1501. 12-Jul-2019 00:37:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:37:36

Channel configuration set to: GFP: 12-Jul-2019 00:37:37

Channel:GFP set at:12-Jul-2019 00:37:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 00:37:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:37:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:37:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.375

Channel configuration set to: Brightfield: 12-Jul-2019 00:37:40

Channel:Brightfield set at:12-Jul-2019 00:37:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 12-Jul-2019 00:37:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:37:41

Channel configuration set to: GFP: 12-Jul-2019 00:37:42

Channel:GFP set at:12-Jul-2019 00:37:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 12-Jul-2019 00:37:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:37:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:37:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.825

Channel configuration set to: Brightfield: 12-Jul-2019 00:37:46

Channel:Brightfield set at:12-Jul-2019 00:37:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 12-Jul-2019 00:37:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:37:46

Channel configuration set to: GFP: 12-Jul-2019 00:37:47

Channel:GFP set at:12-Jul-2019 00:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 12-Jul-2019 00:37:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:37:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:37:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.275

Channel configuration set to: Brightfield: 12-Jul-2019 00:37:51

Channel:Brightfield set at:12-Jul-2019 00:37:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 12-Jul-2019 00:37:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:37:51

Channel configuration set to: GFP: 12-Jul-2019 00:37:53

Channel:GFP set at:12-Jul-2019 00:37:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 12-Jul-2019 00:37:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:37:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:37:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 12-Jul-2019 00:37:56

Channel:Brightfield set at:12-Jul-2019 00:37:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 00:37:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:37:57

Channel configuration set to: GFP: 12-Jul-2019 00:37:58

Channel:GFP set at:12-Jul-2019 00:37:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 00:37:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:37:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.25

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:02

Channel:Brightfield set at:12-Jul-2019 00:38:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 00:38:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:02

Channel configuration set to: GFP: 12-Jul-2019 00:38:03

Channel:GFP set at:12-Jul-2019 00:38:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 00:38:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:06

Channel:Brightfield set at:12-Jul-2019 00:38:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 00:38:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:07

Channel configuration set to: GFP: 12-Jul-2019 00:38:08

Channel:GFP set at:12-Jul-2019 00:38:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 00:38:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:11

Channel:Brightfield set at:12-Jul-2019 00:38:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 00:38:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:12

Channel configuration set to: GFP: 12-Jul-2019 00:38:13

Channel:GFP set at:12-Jul-2019 00:38:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 00:38:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:17

Channel:Brightfield set at:12-Jul-2019 00:38:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 00:38:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:17

Channel configuration set to: GFP: 12-Jul-2019 00:38:18

Channel:GFP set at:12-Jul-2019 00:38:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 00:38:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:22

Channel:Brightfield set at:12-Jul-2019 00:38:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 00:38:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:22

Channel configuration set to: GFP: 12-Jul-2019 00:38:23

Channel:GFP set at:12-Jul-2019 00:38:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 00:38:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.925

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:27

Channel:Brightfield set at:12-Jul-2019 00:38:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 12-Jul-2019 00:38:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:27

Channel configuration set to: GFP: 12-Jul-2019 00:38:28

Channel:GFP set at:12-Jul-2019 00:38:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2251. 12-Jul-2019 00:38:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:32

Channel:Brightfield set at:12-Jul-2019 00:38:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 00:38:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:32

Channel configuration set to: GFP: 12-Jul-2019 00:38:33

Channel:GFP set at:12-Jul-2019 00:38:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 00:38:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:37

Channel:Brightfield set at:12-Jul-2019 00:38:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 00:38:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:37

Channel configuration set to: GFP: 12-Jul-2019 00:38:39

Channel:GFP set at:12-Jul-2019 00:38:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 00:38:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:42

Channel:Brightfield set at:12-Jul-2019 00:38:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 00:38:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:43

Channel configuration set to: GFP: 12-Jul-2019 00:38:44

Channel:GFP set at:12-Jul-2019 00:38:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 00:38:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:47

Channel:Brightfield set at:12-Jul-2019 00:38:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 00:38:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:48

Channel configuration set to: GFP: 12-Jul-2019 00:38:49

Channel:GFP set at:12-Jul-2019 00:38:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 00:38:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:53

Channel:Brightfield set at:12-Jul-2019 00:38:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7751. 12-Jul-2019 00:38:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:53

Channel configuration set to: GFP: 12-Jul-2019 00:38:54

Channel:GFP set at:12-Jul-2019 00:38:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 00:38:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 12-Jul-2019 00:38:58

Channel:Brightfield set at:12-Jul-2019 00:38:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 12-Jul-2019 00:38:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:38:58

Channel configuration set to: GFP: 12-Jul-2019 00:38:59

Channel:GFP set at:12-Jul-2019 00:38:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 12-Jul-2019 00:39:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:39:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:39:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 00:39:03

Channel:Brightfield set at:12-Jul-2019 00:39:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 12-Jul-2019 00:39:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:39:03

Channel configuration set to: GFP: 12-Jul-2019 00:39:04

Channel:GFP set at:12-Jul-2019 00:39:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 12-Jul-2019 00:39:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:39:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:99 completed at:12-Jul-2019 00:39:07

Time since start of timelapse:29493.1364

PFS is locked

Time to next time point:207.4935



------Time point_100------

Memory available for all arrays: 61102985216

Memory used by Matlab: 2211962880

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:42:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 12-Jul-2019 00:42:36

Channel:Brightfield set at:12-Jul-2019 00:42:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 00:42:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:42:36

Channel configuration set to: GFP: 12-Jul-2019 00:42:37

Channel:GFP set at:12-Jul-2019 00:42:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 12-Jul-2019 00:42:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:42:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:42:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.075

Channel configuration set to: Brightfield: 12-Jul-2019 00:42:41

Channel:Brightfield set at:12-Jul-2019 00:42:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7751. 12-Jul-2019 00:42:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:42:41

Channel configuration set to: GFP: 12-Jul-2019 00:42:43

Channel:GFP set at:12-Jul-2019 00:42:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 00:42:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:42:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:42:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 12-Jul-2019 00:42:45

Channel:Brightfield set at:12-Jul-2019 00:42:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 12-Jul-2019 00:42:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:42:46

Channel configuration set to: GFP: 12-Jul-2019 00:42:47

Channel:GFP set at:12-Jul-2019 00:42:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 12-Jul-2019 00:42:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:42:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:42:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.625

Channel configuration set to: Brightfield: 12-Jul-2019 00:42:51

Channel:Brightfield set at:12-Jul-2019 00:42:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 00:42:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:42:51

Channel configuration set to: GFP: 12-Jul-2019 00:42:52

Channel:GFP set at:12-Jul-2019 00:42:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 00:42:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:42:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:42:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 12-Jul-2019 00:42:56

Channel:Brightfield set at:12-Jul-2019 00:42:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 00:42:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:42:56

Channel configuration set to: GFP: 12-Jul-2019 00:42:58

Channel:GFP set at:12-Jul-2019 00:42:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 00:42:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:42:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.175

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:01

Channel:Brightfield set at:12-Jul-2019 00:43:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 00:43:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:02

Channel configuration set to: GFP: 12-Jul-2019 00:43:03

Channel:GFP set at:12-Jul-2019 00:43:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 00:43:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:07

Channel:Brightfield set at:12-Jul-2019 00:43:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 00:43:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:07

Channel configuration set to: GFP: 12-Jul-2019 00:43:08

Channel:GFP set at:12-Jul-2019 00:43:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 00:43:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.425

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:12

Channel:Brightfield set at:12-Jul-2019 00:43:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 12-Jul-2019 00:43:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:13

Channel configuration set to: GFP: 12-Jul-2019 00:43:14

Channel:GFP set at:12-Jul-2019 00:43:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 12-Jul-2019 00:43:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:18

Channel:Brightfield set at:12-Jul-2019 00:43:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 00:43:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:18

Channel configuration set to: GFP: 12-Jul-2019 00:43:19

Channel:GFP set at:12-Jul-2019 00:43:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 00:43:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:22

Channel:Brightfield set at:12-Jul-2019 00:43:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 00:43:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:23

Channel configuration set to: GFP: 12-Jul-2019 00:43:24

Channel:GFP set at:12-Jul-2019 00:43:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 00:43:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:28

Channel:Brightfield set at:12-Jul-2019 00:43:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 00:43:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:28

Channel configuration set to: GFP: 12-Jul-2019 00:43:29

Channel:GFP set at:12-Jul-2019 00:43:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 00:43:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:33

Channel:Brightfield set at:12-Jul-2019 00:43:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 12-Jul-2019 00:43:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:33

Channel configuration set to: GFP: 12-Jul-2019 00:43:35

Channel:GFP set at:12-Jul-2019 00:43:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 12-Jul-2019 00:43:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:39

Channel:Brightfield set at:12-Jul-2019 00:43:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 00:43:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:39

Channel configuration set to: GFP: 12-Jul-2019 00:43:40

Channel:GFP set at:12-Jul-2019 00:43:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 00:43:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:44

Channel:Brightfield set at:12-Jul-2019 00:43:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 00:43:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:45

Channel configuration set to: GFP: 12-Jul-2019 00:43:46

Channel:GFP set at:12-Jul-2019 00:43:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 00:43:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:49

Channel:Brightfield set at:12-Jul-2019 00:43:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 00:43:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:50

Channel configuration set to: GFP: 12-Jul-2019 00:43:51

Channel:GFP set at:12-Jul-2019 00:43:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 00:43:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:55

Channel:Brightfield set at:12-Jul-2019 00:43:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 00:43:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:55

Channel configuration set to: GFP: 12-Jul-2019 00:43:57

Channel:GFP set at:12-Jul-2019 00:43:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7751. 12-Jul-2019 00:43:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:43:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:43:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.275

Channel configuration set to: Brightfield: 12-Jul-2019 00:44:00

Channel:Brightfield set at:12-Jul-2019 00:44:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 12-Jul-2019 00:44:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:44:00

Channel configuration set to: GFP: 12-Jul-2019 00:44:01

Channel:GFP set at:12-Jul-2019 00:44:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 12-Jul-2019 00:44:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:44:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:44:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 00:44:05

Channel:Brightfield set at:12-Jul-2019 00:44:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 00:44:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:44:05

Channel configuration set to: GFP: 12-Jul-2019 00:44:07

Channel:GFP set at:12-Jul-2019 00:44:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 00:44:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:44:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:100 completed at:12-Jul-2019 00:44:09

Time since start of timelapse:29795.5294

PFS is locked

Time to next time point:205.1022



------Time point_101------

Memory available for all arrays: 61095653376

Memory used by Matlab: 2213015552

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:47:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 12-Jul-2019 00:47:36

Channel:Brightfield set at:12-Jul-2019 00:47:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 00:47:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:47:36

Channel configuration set to: GFP: 12-Jul-2019 00:47:38

Channel:GFP set at:12-Jul-2019 00:47:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8751. 12-Jul-2019 00:47:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:47:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:47:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 12-Jul-2019 00:47:41

Channel:Brightfield set at:12-Jul-2019 00:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7251. 12-Jul-2019 00:47:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:47:42

Channel configuration set to: GFP: 12-Jul-2019 00:47:43

Channel:GFP set at:12-Jul-2019 00:47:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 00:47:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:47:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:47:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.9

Channel configuration set to: Brightfield: 12-Jul-2019 00:47:46

Channel:Brightfield set at:12-Jul-2019 00:47:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0501. 12-Jul-2019 00:47:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:47:47

Channel configuration set to: GFP: 12-Jul-2019 00:47:48

Channel:GFP set at:12-Jul-2019 00:47:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0751. 12-Jul-2019 00:47:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:47:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:47:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.675

Channel configuration set to: Brightfield: 12-Jul-2019 00:47:51

Channel:Brightfield set at:12-Jul-2019 00:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 00:47:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:47:52

Channel configuration set to: GFP: 12-Jul-2019 00:47:53

Channel:GFP set at:12-Jul-2019 00:47:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 00:47:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:47:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:47:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.675

Channel configuration set to: Brightfield: 12-Jul-2019 00:47:57

Channel:Brightfield set at:12-Jul-2019 00:47:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 12-Jul-2019 00:47:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:47:57

Channel configuration set to: GFP: 12-Jul-2019 00:47:58

Channel:GFP set at:12-Jul-2019 00:47:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 00:47:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:47:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:01

Channel:Brightfield set at:12-Jul-2019 00:48:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 12-Jul-2019 00:48:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:02

Channel configuration set to: GFP: 12-Jul-2019 00:48:03

Channel:GFP set at:12-Jul-2019 00:48:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 12-Jul-2019 00:48:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:07

Channel:Brightfield set at:12-Jul-2019 00:48:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 00:48:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:08

Channel configuration set to: GFP: 12-Jul-2019 00:48:09

Channel:GFP set at:12-Jul-2019 00:48:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 00:48:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.475

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:13

Channel:Brightfield set at:12-Jul-2019 00:48:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 12-Jul-2019 00:48:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:13

Channel configuration set to: GFP: 12-Jul-2019 00:48:15

Channel:GFP set at:12-Jul-2019 00:48:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 12-Jul-2019 00:48:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:18

Channel:Brightfield set at:12-Jul-2019 00:48:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 00:48:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:19

Channel configuration set to: GFP: 12-Jul-2019 00:48:20

Channel:GFP set at:12-Jul-2019 00:48:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 12-Jul-2019 00:48:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:23

Channel:Brightfield set at:12-Jul-2019 00:48:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 12-Jul-2019 00:48:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:24

Channel configuration set to: GFP: 12-Jul-2019 00:48:25

Channel:GFP set at:12-Jul-2019 00:48:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 12-Jul-2019 00:48:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:28

Channel:Brightfield set at:12-Jul-2019 00:48:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 00:48:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:29

Channel configuration set to: GFP: 12-Jul-2019 00:48:30

Channel:GFP set at:12-Jul-2019 00:48:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 00:48:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:34

Channel:Brightfield set at:12-Jul-2019 00:48:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 00:48:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:34

Channel configuration set to: GFP: 12-Jul-2019 00:48:35

Channel:GFP set at:12-Jul-2019 00:48:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 00:48:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:39

Channel:Brightfield set at:12-Jul-2019 00:48:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 00:48:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:39

Channel configuration set to: GFP: 12-Jul-2019 00:48:41

Channel:GFP set at:12-Jul-2019 00:48:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 00:48:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:43

Channel:Brightfield set at:12-Jul-2019 00:48:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 00:48:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:44

Channel configuration set to: GFP: 12-Jul-2019 00:48:45

Channel:GFP set at:12-Jul-2019 00:48:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 00:48:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:48

Channel:Brightfield set at:12-Jul-2019 00:48:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 00:48:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:49

Channel configuration set to: GFP: 12-Jul-2019 00:48:50

Channel:GFP set at:12-Jul-2019 00:48:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 00:48:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:54

Channel:Brightfield set at:12-Jul-2019 00:48:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 00:48:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:54

Channel configuration set to: GFP: 12-Jul-2019 00:48:55

Channel:GFP set at:12-Jul-2019 00:48:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 00:48:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.35

Channel configuration set to: Brightfield: 12-Jul-2019 00:48:59

Channel:Brightfield set at:12-Jul-2019 00:48:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 00:48:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:48:59

Channel configuration set to: GFP: 12-Jul-2019 00:49:00

Channel:GFP set at:12-Jul-2019 00:49:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 00:49:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:49:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:49:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 12-Jul-2019 00:49:04

Channel:Brightfield set at:12-Jul-2019 00:49:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 00:49:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:49:04

Channel configuration set to: GFP: 12-Jul-2019 00:49:05

Channel:GFP set at:12-Jul-2019 00:49:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 00:49:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:49:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:101 completed at:12-Jul-2019 00:49:08

Time since start of timelapse:30094.1073

PFS is locked

Time to next time point:206.5256



------Time point_102------

Memory available for all arrays: 61100974080

Memory used by Matlab: 2213015552

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:52:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.375

Channel configuration set to: Brightfield: 12-Jul-2019 00:52:36

Channel:Brightfield set at:12-Jul-2019 00:52:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 00:52:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:52:36

Channel configuration set to: GFP: 12-Jul-2019 00:52:37

Channel:GFP set at:12-Jul-2019 00:52:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 00:52:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:52:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:52:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.475

Channel configuration set to: Brightfield: 12-Jul-2019 00:52:40

Channel:Brightfield set at:12-Jul-2019 00:52:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 12-Jul-2019 00:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:52:41

Channel configuration set to: GFP: 12-Jul-2019 00:52:42

Channel:GFP set at:12-Jul-2019 00:52:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 12-Jul-2019 00:52:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:52:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:52:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 12-Jul-2019 00:52:45

Channel:Brightfield set at:12-Jul-2019 00:52:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 00:52:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:52:45

Channel configuration set to: GFP: 12-Jul-2019 00:52:47

Channel:GFP set at:12-Jul-2019 00:52:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 00:52:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:52:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:52:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.7

Channel configuration set to: Brightfield: 12-Jul-2019 00:52:50

Channel:Brightfield set at:12-Jul-2019 00:52:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 00:52:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:52:51

Channel configuration set to: GFP: 12-Jul-2019 00:52:52

Channel:GFP set at:12-Jul-2019 00:52:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 00:52:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:52:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:52:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.825

Channel configuration set to: Brightfield: 12-Jul-2019 00:52:55

Channel:Brightfield set at:12-Jul-2019 00:52:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 00:52:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:52:56

Channel configuration set to: GFP: 12-Jul-2019 00:52:57

Channel:GFP set at:12-Jul-2019 00:52:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 00:52:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:52:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:01

Channel:Brightfield set at:12-Jul-2019 00:53:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 00:53:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:01

Channel configuration set to: GFP: 12-Jul-2019 00:53:02

Channel:GFP set at:12-Jul-2019 00:53:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 00:53:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:06

Channel:Brightfield set at:12-Jul-2019 00:53:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 00:53:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:06

Channel configuration set to: GFP: 12-Jul-2019 00:53:08

Channel:GFP set at:12-Jul-2019 00:53:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 00:53:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:11

Channel:Brightfield set at:12-Jul-2019 00:53:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2751. 12-Jul-2019 00:53:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:12

Channel configuration set to: GFP: 12-Jul-2019 00:53:13

Channel:GFP set at:12-Jul-2019 00:53:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3001. 12-Jul-2019 00:53:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.8

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:16

Channel:Brightfield set at:12-Jul-2019 00:53:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 12-Jul-2019 00:53:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:17

Channel configuration set to: GFP: 12-Jul-2019 00:53:18

Channel:GFP set at:12-Jul-2019 00:53:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 12-Jul-2019 00:53:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:22

Channel:Brightfield set at:12-Jul-2019 00:53:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 12-Jul-2019 00:53:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:22

Channel configuration set to: GFP: 12-Jul-2019 00:53:23

Channel:GFP set at:12-Jul-2019 00:53:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 12-Jul-2019 00:53:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:27

Channel:Brightfield set at:12-Jul-2019 00:53:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 00:53:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:27

Channel configuration set to: GFP: 12-Jul-2019 00:53:28

Channel:GFP set at:12-Jul-2019 00:53:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 00:53:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:32

Channel:Brightfield set at:12-Jul-2019 00:53:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 00:53:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:32

Channel configuration set to: GFP: 12-Jul-2019 00:53:33

Channel:GFP set at:12-Jul-2019 00:53:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 00:53:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:37

Channel:Brightfield set at:12-Jul-2019 00:53:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 00:53:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:37

Channel configuration set to: GFP: 12-Jul-2019 00:53:39

Channel:GFP set at:12-Jul-2019 00:53:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 00:53:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:41

Channel:Brightfield set at:12-Jul-2019 00:53:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 00:53:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:42

Channel configuration set to: GFP: 12-Jul-2019 00:53:43

Channel:GFP set at:12-Jul-2019 00:53:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 00:53:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:43

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:46

Channel:Brightfield set at:12-Jul-2019 00:53:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 00:53:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:47

Channel configuration set to: GFP: 12-Jul-2019 00:53:48

Channel:GFP set at:12-Jul-2019 00:53:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 00:53:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:52

Channel:Brightfield set at:12-Jul-2019 00:53:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 00:53:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:52

Channel configuration set to: GFP: 12-Jul-2019 00:53:54

Channel:GFP set at:12-Jul-2019 00:53:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 00:53:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 12-Jul-2019 00:53:57

Channel:Brightfield set at:12-Jul-2019 00:53:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 00:53:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:57

Channel configuration set to: GFP: 12-Jul-2019 00:53:59

Channel:GFP set at:12-Jul-2019 00:53:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 00:53:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:53:59

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:54:01

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 12-Jul-2019 00:54:02

Channel:Brightfield set at:12-Jul-2019 00:54:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 00:54:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:54:03

Channel configuration set to: GFP: 12-Jul-2019 00:54:04

Channel:GFP set at:12-Jul-2019 00:54:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 00:54:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:54:04

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:102 completed at:12-Jul-2019 00:54:06

Time since start of timelapse:30392.7637

PFS is locked

Time to next time point:207.8709



------Time point_103------

Memory available for all arrays: 61100486656

Memory used by Matlab: 2213015552

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:57:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.375

Channel configuration set to: Brightfield: 12-Jul-2019 00:57:36

Channel:Brightfield set at:12-Jul-2019 00:57:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 00:57:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:57:36

Channel configuration set to: GFP: 12-Jul-2019 00:57:37

Channel:GFP set at:12-Jul-2019 00:57:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 00:57:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:57:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:57:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.3

Channel configuration set to: Brightfield: 12-Jul-2019 00:57:41

Channel:Brightfield set at:12-Jul-2019 00:57:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 00:57:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:57:41

Channel configuration set to: GFP: 12-Jul-2019 00:57:42

Channel:GFP set at:12-Jul-2019 00:57:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 00:57:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:57:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:57:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 12-Jul-2019 00:57:45

Channel:Brightfield set at:12-Jul-2019 00:57:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 00:57:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:57:46

Channel configuration set to: GFP: 12-Jul-2019 00:57:47

Channel:GFP set at:12-Jul-2019 00:57:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 00:57:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:57:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:57:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.75

Channel configuration set to: Brightfield: 12-Jul-2019 00:57:50

Channel:Brightfield set at:12-Jul-2019 00:57:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 00:57:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:57:51

Channel configuration set to: GFP: 12-Jul-2019 00:57:52

Channel:GFP set at:12-Jul-2019 00:57:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 00:57:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:57:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:57:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.825

Channel configuration set to: Brightfield: 12-Jul-2019 00:57:56

Channel:Brightfield set at:12-Jul-2019 00:57:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 00:57:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:57:56

Channel configuration set to: GFP: 12-Jul-2019 00:57:57

Channel:GFP set at:12-Jul-2019 00:57:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 00:57:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:57:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.425

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:00

Channel:Brightfield set at:12-Jul-2019 00:58:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 00:58:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:01

Channel configuration set to: GFP: 12-Jul-2019 00:58:02

Channel:GFP set at:12-Jul-2019 00:58:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 00:58:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:06

Channel:Brightfield set at:12-Jul-2019 00:58:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 00:58:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:07

Channel configuration set to: GFP: 12-Jul-2019 00:58:08

Channel:GFP set at:12-Jul-2019 00:58:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 00:58:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:12

Channel:Brightfield set at:12-Jul-2019 00:58:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3001. 12-Jul-2019 00:58:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:12

Channel configuration set to: GFP: 12-Jul-2019 00:58:14

Channel:GFP set at:12-Jul-2019 00:58:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2751. 12-Jul-2019 00:58:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.1

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:17

Channel:Brightfield set at:12-Jul-2019 00:58:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 00:58:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:18

Channel configuration set to: GFP: 12-Jul-2019 00:58:19

Channel:GFP set at:12-Jul-2019 00:58:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 00:58:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:22

Channel:Brightfield set at:12-Jul-2019 00:58:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 12-Jul-2019 00:58:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:22

Channel configuration set to: GFP: 12-Jul-2019 00:58:24

Channel:GFP set at:12-Jul-2019 00:58:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 12-Jul-2019 00:58:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:27

Channel:Brightfield set at:12-Jul-2019 00:58:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 00:58:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:28

Channel configuration set to: GFP: 12-Jul-2019 00:58:29

Channel:GFP set at:12-Jul-2019 00:58:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 00:58:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:33

Channel:Brightfield set at:12-Jul-2019 00:58:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 00:58:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:33

Channel configuration set to: GFP: 12-Jul-2019 00:58:35

Channel:GFP set at:12-Jul-2019 00:58:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 00:58:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:39

Channel:Brightfield set at:12-Jul-2019 00:58:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 12-Jul-2019 00:58:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:39

Channel configuration set to: GFP: 12-Jul-2019 00:58:40

Channel:GFP set at:12-Jul-2019 00:58:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 12-Jul-2019 00:58:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:44

Channel:Brightfield set at:12-Jul-2019 00:58:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 00:58:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:45

Channel configuration set to: GFP: 12-Jul-2019 00:58:46

Channel:GFP set at:12-Jul-2019 00:58:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 00:58:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:49

Channel:Brightfield set at:12-Jul-2019 00:58:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 00:58:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:50

Channel configuration set to: GFP: 12-Jul-2019 00:58:51

Channel:GFP set at:12-Jul-2019 00:58:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 00:58:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.775

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:54

Channel:Brightfield set at:12-Jul-2019 00:58:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 12-Jul-2019 00:58:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:55

Channel configuration set to: GFP: 12-Jul-2019 00:58:56

Channel:GFP set at:12-Jul-2019 00:58:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 12-Jul-2019 00:58:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:58:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 12-Jul-2019 00:58:59

Channel:Brightfield set at:12-Jul-2019 00:58:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 00:59:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:59:00

Channel configuration set to: GFP: 12-Jul-2019 00:59:01

Channel:GFP set at:12-Jul-2019 00:59:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 00:59:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:59:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 00:59:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 00:59:05

Channel:Brightfield set at:12-Jul-2019 00:59:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 12-Jul-2019 00:59:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:59:06

Channel configuration set to: GFP: 12-Jul-2019 00:59:07

Channel:GFP set at:12-Jul-2019 00:59:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 12-Jul-2019 00:59:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 00:59:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:103 completed at:12-Jul-2019 00:59:09

Time since start of timelapse:30695.7684

PFS is locked

Time to next time point:204.8676



------Time point_104------

Memory available for all arrays: 60263723008

Memory used by Matlab: 2213052416

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:02:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 12-Jul-2019 01:02:35

Channel:Brightfield set at:12-Jul-2019 01:02:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 01:02:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:02:36

Channel configuration set to: GFP: 12-Jul-2019 01:02:37

Channel:GFP set at:12-Jul-2019 01:02:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 01:02:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:02:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:02:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 12-Jul-2019 01:02:41

Channel:Brightfield set at:12-Jul-2019 01:02:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7251. 12-Jul-2019 01:02:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:02:41

Channel configuration set to: GFP: 12-Jul-2019 01:02:42

Channel:GFP set at:12-Jul-2019 01:02:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 01:02:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:02:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:02:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.925

Channel configuration set to: Brightfield: 12-Jul-2019 01:02:46

Channel:Brightfield set at:12-Jul-2019 01:02:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0001. 12-Jul-2019 01:02:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:02:46

Channel configuration set to: GFP: 12-Jul-2019 01:02:47

Channel:GFP set at:12-Jul-2019 01:02:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.0251. 12-Jul-2019 01:02:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:02:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:02:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.725

Channel configuration set to: Brightfield: 12-Jul-2019 01:02:51

Channel:Brightfield set at:12-Jul-2019 01:02:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 01:02:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:02:51

Channel configuration set to: GFP: 12-Jul-2019 01:02:53

Channel:GFP set at:12-Jul-2019 01:02:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 01:02:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:02:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:02:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.725

Channel configuration set to: Brightfield: 12-Jul-2019 01:02:56

Channel:Brightfield set at:12-Jul-2019 01:02:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 01:02:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:02:56

Channel configuration set to: GFP: 12-Jul-2019 01:02:58

Channel:GFP set at:12-Jul-2019 01:02:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 01:02:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:02:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.85

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:01

Channel:Brightfield set at:12-Jul-2019 01:03:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 01:03:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:02

Channel configuration set to: GFP: 12-Jul-2019 01:03:03

Channel:GFP set at:12-Jul-2019 01:03:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 01:03:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:07

Channel:Brightfield set at:12-Jul-2019 01:03:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 01:03:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:07

Channel configuration set to: GFP: 12-Jul-2019 01:03:08

Channel:GFP set at:12-Jul-2019 01:03:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 01:03:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.475

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:12

Channel:Brightfield set at:12-Jul-2019 01:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 12-Jul-2019 01:03:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:13

Channel configuration set to: GFP: 12-Jul-2019 01:03:14

Channel:GFP set at:12-Jul-2019 01:03:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 12-Jul-2019 01:03:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.9

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:18

Channel:Brightfield set at:12-Jul-2019 01:03:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 01:03:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:18

Channel configuration set to: GFP: 12-Jul-2019 01:03:19

Channel:GFP set at:12-Jul-2019 01:03:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 01:03:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:23

Channel:Brightfield set at:12-Jul-2019 01:03:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 01:03:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:23

Channel configuration set to: GFP: 12-Jul-2019 01:03:25

Channel:GFP set at:12-Jul-2019 01:03:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 01:03:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:28

Channel:Brightfield set at:12-Jul-2019 01:03:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 01:03:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:29

Channel configuration set to: GFP: 12-Jul-2019 01:03:30

Channel:GFP set at:12-Jul-2019 01:03:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 01:03:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:34

Channel:Brightfield set at:12-Jul-2019 01:03:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 12-Jul-2019 01:03:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:34

Channel configuration set to: GFP: 12-Jul-2019 01:03:35

Channel:GFP set at:12-Jul-2019 01:03:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 12-Jul-2019 01:03:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:39

Channel:Brightfield set at:12-Jul-2019 01:03:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 01:03:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:39

Channel configuration set to: GFP: 12-Jul-2019 01:03:41

Channel:GFP set at:12-Jul-2019 01:03:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 01:03:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:44

Channel:Brightfield set at:12-Jul-2019 01:03:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 01:03:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:45

Channel configuration set to: GFP: 12-Jul-2019 01:03:46

Channel:GFP set at:12-Jul-2019 01:03:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 01:03:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:49

Channel:Brightfield set at:12-Jul-2019 01:03:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 01:03:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:49

Channel configuration set to: GFP: 12-Jul-2019 01:03:51

Channel:GFP set at:12-Jul-2019 01:03:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 01:03:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:54

Channel:Brightfield set at:12-Jul-2019 01:03:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 01:03:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:55

Channel configuration set to: GFP: 12-Jul-2019 01:03:56

Channel:GFP set at:12-Jul-2019 01:03:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 01:03:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:03:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.35

Channel configuration set to: Brightfield: 12-Jul-2019 01:03:59

Channel:Brightfield set at:12-Jul-2019 01:03:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 12-Jul-2019 01:04:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:04:00

Channel configuration set to: GFP: 12-Jul-2019 01:04:01

Channel:GFP set at:12-Jul-2019 01:04:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 01:04:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:04:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:04:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 01:04:05

Channel:Brightfield set at:12-Jul-2019 01:04:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 01:04:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:04:05

Channel configuration set to: GFP: 12-Jul-2019 01:04:06

Channel:GFP set at:12-Jul-2019 01:04:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 01:04:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:04:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:104 completed at:12-Jul-2019 01:04:09

Time since start of timelapse:30995.2517

PFS is locked

Time to next time point:205.3858



------Time point_105------

Memory available for all arrays: 61048639488

Memory used by Matlab: 2213076992

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:07:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.6

Channel configuration set to: Brightfield: 12-Jul-2019 01:07:36

Channel:Brightfield set at:12-Jul-2019 01:07:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 01:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:07:36

Channel configuration set to: GFP: 12-Jul-2019 01:07:37

Channel:GFP set at:12-Jul-2019 01:07:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 01:07:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:07:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:07:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 12-Jul-2019 01:07:41

Channel:Brightfield set at:12-Jul-2019 01:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 01:07:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:07:41

Channel configuration set to: GFP: 12-Jul-2019 01:07:42

Channel:GFP set at:12-Jul-2019 01:07:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 01:07:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:07:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:07:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.35

Channel configuration set to: Brightfield: 12-Jul-2019 01:07:45

Channel:Brightfield set at:12-Jul-2019 01:07:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 01:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:07:46

Channel configuration set to: GFP: 12-Jul-2019 01:07:47

Channel:GFP set at:12-Jul-2019 01:07:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 01:07:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:07:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:07:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.925

Channel configuration set to: Brightfield: 12-Jul-2019 01:07:50

Channel:Brightfield set at:12-Jul-2019 01:07:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 01:07:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:07:51

Channel configuration set to: GFP: 12-Jul-2019 01:07:52

Channel:GFP set at:12-Jul-2019 01:07:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 01:07:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:07:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:07:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.95

Channel configuration set to: Brightfield: 12-Jul-2019 01:07:56

Channel:Brightfield set at:12-Jul-2019 01:07:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 01:07:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:07:56

Channel configuration set to: GFP: 12-Jul-2019 01:07:57

Channel:GFP set at:12-Jul-2019 01:07:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 01:07:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:07:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.5

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:01

Channel:Brightfield set at:12-Jul-2019 01:08:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9251. 12-Jul-2019 01:08:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:01

Channel configuration set to: GFP: 12-Jul-2019 01:08:02

Channel:GFP set at:12-Jul-2019 01:08:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 12-Jul-2019 01:08:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:06

Channel:Brightfield set at:12-Jul-2019 01:08:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 01:08:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:07

Channel configuration set to: GFP: 12-Jul-2019 01:08:08

Channel:GFP set at:12-Jul-2019 01:08:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 01:08:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:12

Channel:Brightfield set at:12-Jul-2019 01:08:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 12-Jul-2019 01:08:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:12

Channel configuration set to: GFP: 12-Jul-2019 01:08:14

Channel:GFP set at:12-Jul-2019 01:08:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 12-Jul-2019 01:08:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:17

Channel:Brightfield set at:12-Jul-2019 01:08:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 12-Jul-2019 01:08:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:17

Channel configuration set to: GFP: 12-Jul-2019 01:08:18

Channel:GFP set at:12-Jul-2019 01:08:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5001. 12-Jul-2019 01:08:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.425

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:22

Channel:Brightfield set at:12-Jul-2019 01:08:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 12-Jul-2019 01:08:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:23

Channel configuration set to: GFP: 12-Jul-2019 01:08:24

Channel:GFP set at:12-Jul-2019 01:08:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 12-Jul-2019 01:08:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:28

Channel:Brightfield set at:12-Jul-2019 01:08:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 01:08:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:28

Channel configuration set to: GFP: 12-Jul-2019 01:08:29

Channel:GFP set at:12-Jul-2019 01:08:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 01:08:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:33

Channel:Brightfield set at:12-Jul-2019 01:08:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 01:08:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:33

Channel configuration set to: GFP: 12-Jul-2019 01:08:34

Channel:GFP set at:12-Jul-2019 01:08:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 01:08:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:38

Channel:Brightfield set at:12-Jul-2019 01:08:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 01:08:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:38

Channel configuration set to: GFP: 12-Jul-2019 01:08:39

Channel:GFP set at:12-Jul-2019 01:08:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 01:08:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:42

Channel:Brightfield set at:12-Jul-2019 01:08:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 01:08:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:43

Channel configuration set to: GFP: 12-Jul-2019 01:08:44

Channel:GFP set at:12-Jul-2019 01:08:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 01:08:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:47

Channel:Brightfield set at:12-Jul-2019 01:08:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 01:08:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:48

Channel configuration set to: GFP: 12-Jul-2019 01:08:49

Channel:GFP set at:12-Jul-2019 01:08:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 01:08:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:53

Channel:Brightfield set at:12-Jul-2019 01:08:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 01:08:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:53

Channel configuration set to: GFP: 12-Jul-2019 01:08:54

Channel:GFP set at:12-Jul-2019 01:08:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 01:08:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.475

Channel configuration set to: Brightfield: 12-Jul-2019 01:08:58

Channel:Brightfield set at:12-Jul-2019 01:08:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 12-Jul-2019 01:08:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:08:58

Channel configuration set to: GFP: 12-Jul-2019 01:08:59

Channel:GFP set at:12-Jul-2019 01:08:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 12-Jul-2019 01:09:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:09:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:09:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:09:03

Channel:Brightfield set at:12-Jul-2019 01:09:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 12-Jul-2019 01:09:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:09:03

Channel configuration set to: GFP: 12-Jul-2019 01:09:04

Channel:GFP set at:12-Jul-2019 01:09:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 12-Jul-2019 01:09:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:09:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:105 completed at:12-Jul-2019 01:09:07

Time since start of timelapse:31293.2944

PFS is locked

Time to next time point:207.3448



------Time point_106------

Memory available for all arrays: 61074673664

Memory used by Matlab: 2213076992

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:12:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 12-Jul-2019 01:12:36

Channel:Brightfield set at:12-Jul-2019 01:12:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 01:12:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:12:36

Channel configuration set to: GFP: 12-Jul-2019 01:12:37

Channel:GFP set at:12-Jul-2019 01:12:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 01:12:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:12:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:12:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 12-Jul-2019 01:12:41

Channel:Brightfield set at:12-Jul-2019 01:12:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 01:12:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:12:41

Channel configuration set to: GFP: 12-Jul-2019 01:12:43

Channel:GFP set at:12-Jul-2019 01:12:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 01:12:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:12:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:12:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.2

Channel configuration set to: Brightfield: 12-Jul-2019 01:12:46

Channel:Brightfield set at:12-Jul-2019 01:12:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 12-Jul-2019 01:12:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:12:47

Channel configuration set to: GFP: 12-Jul-2019 01:12:48

Channel:GFP set at:12-Jul-2019 01:12:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 12-Jul-2019 01:12:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:12:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:12:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.9

Channel configuration set to: Brightfield: 12-Jul-2019 01:12:52

Channel:Brightfield set at:12-Jul-2019 01:12:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 01:12:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:12:52

Channel configuration set to: GFP: 12-Jul-2019 01:12:54

Channel:GFP set at:12-Jul-2019 01:12:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 01:12:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:12:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:12:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.95

Channel configuration set to: Brightfield: 12-Jul-2019 01:12:57

Channel:Brightfield set at:12-Jul-2019 01:12:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 01:12:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:12:58

Channel configuration set to: GFP: 12-Jul-2019 01:12:59

Channel:GFP set at:12-Jul-2019 01:12:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 01:12:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:12:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.5

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:02

Channel:Brightfield set at:12-Jul-2019 01:13:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 01:13:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:02

Channel configuration set to: GFP: 12-Jul-2019 01:13:04

Channel:GFP set at:12-Jul-2019 01:13:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 01:13:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:07

Channel:Brightfield set at:12-Jul-2019 01:13:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5001. 12-Jul-2019 01:13:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:08

Channel configuration set to: GFP: 12-Jul-2019 01:13:09

Channel:GFP set at:12-Jul-2019 01:13:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 12-Jul-2019 01:13:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.775

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:13

Channel:Brightfield set at:12-Jul-2019 01:13:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 01:13:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:14

Channel configuration set to: GFP: 12-Jul-2019 01:13:15

Channel:GFP set at:12-Jul-2019 01:13:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 01:13:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.275

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:19

Channel:Brightfield set at:12-Jul-2019 01:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 01:13:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:19

Channel configuration set to: GFP: 12-Jul-2019 01:13:20

Channel:GFP set at:12-Jul-2019 01:13:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 01:13:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:23

Channel:Brightfield set at:12-Jul-2019 01:13:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 12-Jul-2019 01:13:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:24

Channel configuration set to: GFP: 12-Jul-2019 01:13:25

Channel:GFP set at:12-Jul-2019 01:13:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 01:13:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.5

PFS not locked. Waiting for lock...

PFS took 0.200000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:28

Channel:Brightfield set at:12-Jul-2019 01:13:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8251. 12-Jul-2019 01:13:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:29

Channel configuration set to: GFP: 12-Jul-2019 01:13:30

Channel:GFP set at:12-Jul-2019 01:13:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8251. 12-Jul-2019 01:13:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:34

Channel:Brightfield set at:12-Jul-2019 01:13:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 01:13:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:34

Channel configuration set to: GFP: 12-Jul-2019 01:13:36

Channel:GFP set at:12-Jul-2019 01:13:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 01:13:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:39

Channel:Brightfield set at:12-Jul-2019 01:13:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 01:13:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:39

Channel configuration set to: GFP: 12-Jul-2019 01:13:41

Channel:GFP set at:12-Jul-2019 01:13:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 01:13:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:44

Channel:Brightfield set at:12-Jul-2019 01:13:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 01:13:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:45

Channel configuration set to: GFP: 12-Jul-2019 01:13:46

Channel:GFP set at:12-Jul-2019 01:13:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 01:13:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:49

Channel:Brightfield set at:12-Jul-2019 01:13:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 01:13:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:50

Channel configuration set to: GFP: 12-Jul-2019 01:13:51

Channel:GFP set at:12-Jul-2019 01:13:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 01:13:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.925

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:54

Channel:Brightfield set at:12-Jul-2019 01:13:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 12-Jul-2019 01:13:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:55

Channel configuration set to: GFP: 12-Jul-2019 01:13:56

Channel:GFP set at:12-Jul-2019 01:13:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 01:13:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 12-Jul-2019 01:13:59

Channel:Brightfield set at:12-Jul-2019 01:13:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 01:13:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:13:59

Channel configuration set to: GFP: 12-Jul-2019 01:14:01

Channel:GFP set at:12-Jul-2019 01:14:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 01:14:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:14:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:14:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 12-Jul-2019 01:14:04

Channel:Brightfield set at:12-Jul-2019 01:14:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 01:14:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:14:05

Channel configuration set to: GFP: 12-Jul-2019 01:14:06

Channel:GFP set at:12-Jul-2019 01:14:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 12-Jul-2019 01:14:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:14:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:106 completed at:12-Jul-2019 01:14:08

Time since start of timelapse:31594.745

PFS is locked

Time to next time point:205.8954



------Time point_107------

Memory available for all arrays: 61078736896

Memory used by Matlab: 2213076992

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:17:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.375

Channel configuration set to: Brightfield: 12-Jul-2019 01:17:35

Channel:Brightfield set at:12-Jul-2019 01:17:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 01:17:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:17:36

Channel configuration set to: GFP: 12-Jul-2019 01:17:37

Channel:GFP set at:12-Jul-2019 01:17:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 01:17:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:17:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:17:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:17:41

Channel:Brightfield set at:12-Jul-2019 01:17:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6251. 12-Jul-2019 01:17:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:17:41

Channel configuration set to: GFP: 12-Jul-2019 01:17:43

Channel:GFP set at:12-Jul-2019 01:17:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6251. 12-Jul-2019 01:17:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:17:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:17:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:17:45

Channel:Brightfield set at:12-Jul-2019 01:17:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 01:17:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:17:46

Channel configuration set to: GFP: 12-Jul-2019 01:17:47

Channel:GFP set at:12-Jul-2019 01:17:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 01:17:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:17:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:17:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.675

Channel configuration set to: Brightfield: 12-Jul-2019 01:17:51

Channel:Brightfield set at:12-Jul-2019 01:17:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 12-Jul-2019 01:17:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:17:51

Channel configuration set to: GFP: 12-Jul-2019 01:17:52

Channel:GFP set at:12-Jul-2019 01:17:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 12-Jul-2019 01:17:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:17:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:17:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.725

Channel configuration set to: Brightfield: 12-Jul-2019 01:17:56

Channel:Brightfield set at:12-Jul-2019 01:17:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 01:17:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:17:57

Channel configuration set to: GFP: 12-Jul-2019 01:17:58

Channel:GFP set at:12-Jul-2019 01:17:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 01:17:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:17:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.4

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:01

Channel:Brightfield set at:12-Jul-2019 01:18:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 01:18:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:01

Channel configuration set to: GFP: 12-Jul-2019 01:18:03

Channel:GFP set at:12-Jul-2019 01:18:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 01:18:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:06

Channel:Brightfield set at:12-Jul-2019 01:18:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 01:18:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:07

Channel configuration set to: GFP: 12-Jul-2019 01:18:08

Channel:GFP set at:12-Jul-2019 01:18:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 01:18:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:11

Channel:Brightfield set at:12-Jul-2019 01:18:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 01:18:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:12

Channel configuration set to: GFP: 12-Jul-2019 01:18:13

Channel:GFP set at:12-Jul-2019 01:18:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 01:18:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:17

Channel:Brightfield set at:12-Jul-2019 01:18:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 01:18:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:17

Channel configuration set to: GFP: 12-Jul-2019 01:18:19

Channel:GFP set at:12-Jul-2019 01:18:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 01:18:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:22

Channel:Brightfield set at:12-Jul-2019 01:18:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 12-Jul-2019 01:18:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:22

Channel configuration set to: GFP: 12-Jul-2019 01:18:24

Channel:GFP set at:12-Jul-2019 01:18:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 12-Jul-2019 01:18:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:27

Channel:Brightfield set at:12-Jul-2019 01:18:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 01:18:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:28

Channel configuration set to: GFP: 12-Jul-2019 01:18:29

Channel:GFP set at:12-Jul-2019 01:18:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 01:18:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:32

Channel:Brightfield set at:12-Jul-2019 01:18:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 01:18:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:33

Channel configuration set to: GFP: 12-Jul-2019 01:18:34

Channel:GFP set at:12-Jul-2019 01:18:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 01:18:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:38

Channel:Brightfield set at:12-Jul-2019 01:18:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 01:18:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:39

Channel configuration set to: GFP: 12-Jul-2019 01:18:40

Channel:GFP set at:12-Jul-2019 01:18:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 01:18:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:44

Channel:Brightfield set at:12-Jul-2019 01:18:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 01:18:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:44

Channel configuration set to: GFP: 12-Jul-2019 01:18:46

Channel:GFP set at:12-Jul-2019 01:18:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 01:18:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:49

Channel:Brightfield set at:12-Jul-2019 01:18:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 01:18:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:49

Channel configuration set to: GFP: 12-Jul-2019 01:18:50

Channel:GFP set at:12-Jul-2019 01:18:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 01:18:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:54

Channel:Brightfield set at:12-Jul-2019 01:18:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 01:18:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:55

Channel configuration set to: GFP: 12-Jul-2019 01:18:56

Channel:GFP set at:12-Jul-2019 01:18:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 01:18:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.35

Channel configuration set to: Brightfield: 12-Jul-2019 01:18:59

Channel:Brightfield set at:12-Jul-2019 01:18:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 12-Jul-2019 01:18:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:18:59

Channel configuration set to: GFP: 12-Jul-2019 01:19:01

Channel:GFP set at:12-Jul-2019 01:19:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 12-Jul-2019 01:19:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:19:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:19:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 12-Jul-2019 01:19:04

Channel:Brightfield set at:12-Jul-2019 01:19:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 01:19:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:19:05

Channel configuration set to: GFP: 12-Jul-2019 01:19:06

Channel:GFP set at:12-Jul-2019 01:19:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 01:19:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:19:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:107 completed at:12-Jul-2019 01:19:08

Time since start of timelapse:31894.9384

PFS is locked

Time to next time point:205.7036



------Time point_108------

Memory available for all arrays: 61071728640

Memory used by Matlab: 2213076992

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:22:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 01:22:35

Channel:Brightfield set at:12-Jul-2019 01:22:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 01:22:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:22:36

Channel configuration set to: GFP: 12-Jul-2019 01:22:37

Channel:GFP set at:12-Jul-2019 01:22:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 01:22:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:22:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:22:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.05

Channel configuration set to: Brightfield: 12-Jul-2019 01:22:41

Channel:Brightfield set at:12-Jul-2019 01:22:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 01:22:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:22:41

Channel configuration set to: GFP: 12-Jul-2019 01:22:42

Channel:GFP set at:12-Jul-2019 01:22:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 01:22:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:22:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:22:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.825

Channel configuration set to: Brightfield: 12-Jul-2019 01:22:46

Channel:Brightfield set at:12-Jul-2019 01:22:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1251. 12-Jul-2019 01:22:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:22:46

Channel configuration set to: GFP: 12-Jul-2019 01:22:48

Channel:GFP set at:12-Jul-2019 01:22:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 12-Jul-2019 01:22:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:22:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:22:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.625

Channel configuration set to: Brightfield: 12-Jul-2019 01:22:51

Channel:Brightfield set at:12-Jul-2019 01:22:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 01:22:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:22:51

Channel configuration set to: GFP: 12-Jul-2019 01:22:53

Channel:GFP set at:12-Jul-2019 01:22:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 12-Jul-2019 01:22:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:22:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:22:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.6

PFS not locked. Waiting for lock...

PFS took 0.450000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:22:57

Channel:Brightfield set at:12-Jul-2019 01:22:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 01:22:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:22:57

Channel configuration set to: GFP: 12-Jul-2019 01:22:58

Channel:GFP set at:12-Jul-2019 01:22:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 01:22:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:22:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:02

Channel:Brightfield set at:12-Jul-2019 01:23:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 01:23:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:03

Channel configuration set to: GFP: 12-Jul-2019 01:23:04

Channel:GFP set at:12-Jul-2019 01:23:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 01:23:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:07

Channel:Brightfield set at:12-Jul-2019 01:23:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 01:23:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:07

Channel configuration set to: GFP: 12-Jul-2019 01:23:09

Channel:GFP set at:12-Jul-2019 01:23:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 01:23:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:12

Channel:Brightfield set at:12-Jul-2019 01:23:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 01:23:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:12

Channel configuration set to: GFP: 12-Jul-2019 01:23:14

Channel:GFP set at:12-Jul-2019 01:23:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 01:23:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.775

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:16

Channel:Brightfield set at:12-Jul-2019 01:23:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 01:23:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:17

Channel configuration set to: GFP: 12-Jul-2019 01:23:18

Channel:GFP set at:12-Jul-2019 01:23:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 01:23:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:21

Channel:Brightfield set at:12-Jul-2019 01:23:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3001. 12-Jul-2019 01:23:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:22

Channel configuration set to: GFP: 12-Jul-2019 01:23:23

Channel:GFP set at:12-Jul-2019 01:23:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2751. 12-Jul-2019 01:23:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:27

Channel:Brightfield set at:12-Jul-2019 01:23:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 12-Jul-2019 01:23:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:28

Channel configuration set to: GFP: 12-Jul-2019 01:23:29

Channel:GFP set at:12-Jul-2019 01:23:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 01:23:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:32

Channel:Brightfield set at:12-Jul-2019 01:23:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 01:23:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:32

Channel configuration set to: GFP: 12-Jul-2019 01:23:34

Channel:GFP set at:12-Jul-2019 01:23:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 01:23:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:38

Channel:Brightfield set at:12-Jul-2019 01:23:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 01:23:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:38

Channel configuration set to: GFP: 12-Jul-2019 01:23:39

Channel:GFP set at:12-Jul-2019 01:23:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 01:23:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:43

Channel:Brightfield set at:12-Jul-2019 01:23:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 01:23:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:44

Channel configuration set to: GFP: 12-Jul-2019 01:23:45

Channel:GFP set at:12-Jul-2019 01:23:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 01:23:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:48

Channel:Brightfield set at:12-Jul-2019 01:23:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 01:23:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:48

Channel configuration set to: GFP: 12-Jul-2019 01:23:50

Channel:GFP set at:12-Jul-2019 01:23:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 01:23:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.325

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:54

Channel:Brightfield set at:12-Jul-2019 01:23:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 01:23:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:54

Channel configuration set to: GFP: 12-Jul-2019 01:23:55

Channel:GFP set at:12-Jul-2019 01:23:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 01:23:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.025

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:23:59

Channel:Brightfield set at:12-Jul-2019 01:23:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 01:23:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:23:59

Channel configuration set to: GFP: 12-Jul-2019 01:24:01

Channel:GFP set at:12-Jul-2019 01:24:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 01:24:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:24:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:24:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 01:24:04

Channel:Brightfield set at:12-Jul-2019 01:24:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 01:24:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:24:04

Channel configuration set to: GFP: 12-Jul-2019 01:24:06

Channel:GFP set at:12-Jul-2019 01:24:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 01:24:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:24:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:108 completed at:12-Jul-2019 01:24:08

Time since start of timelapse:32194.7321

PFS is locked

Time to next time point:205.9111



------Time point_109------

Memory available for all arrays: 61071155200

Memory used by Matlab: 2213076992

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:27:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 12-Jul-2019 01:27:35

Channel:Brightfield set at:12-Jul-2019 01:27:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1501. 12-Jul-2019 01:27:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:27:36

Channel configuration set to: GFP: 12-Jul-2019 01:27:37

Channel:GFP set at:12-Jul-2019 01:27:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1501. 12-Jul-2019 01:27:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:27:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:27:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.775

Channel configuration set to: Brightfield: 12-Jul-2019 01:27:41

Channel:Brightfield set at:12-Jul-2019 01:27:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 01:27:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:27:41

Channel configuration set to: GFP: 12-Jul-2019 01:27:42

Channel:GFP set at:12-Jul-2019 01:27:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 01:27:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:27:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:27:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 12-Jul-2019 01:27:46

Channel:Brightfield set at:12-Jul-2019 01:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 12-Jul-2019 01:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:27:46

Channel configuration set to: GFP: 12-Jul-2019 01:27:47

Channel:GFP set at:12-Jul-2019 01:27:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3385.0501. 12-Jul-2019 01:27:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:27:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:27:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.575

Channel configuration set to: Brightfield: 12-Jul-2019 01:27:51

Channel:Brightfield set at:12-Jul-2019 01:27:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 12-Jul-2019 01:27:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:27:51

Channel configuration set to: GFP: 12-Jul-2019 01:27:52

Channel:GFP set at:12-Jul-2019 01:27:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 12-Jul-2019 01:27:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:27:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:27:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.55

Channel configuration set to: Brightfield: 12-Jul-2019 01:27:56

Channel:Brightfield set at:12-Jul-2019 01:27:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 01:27:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:27:56

Channel configuration set to: GFP: 12-Jul-2019 01:27:58

Channel:GFP set at:12-Jul-2019 01:27:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 01:27:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:27:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:02

Channel:Brightfield set at:12-Jul-2019 01:28:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 01:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:02

Channel configuration set to: GFP: 12-Jul-2019 01:28:03

Channel:GFP set at:12-Jul-2019 01:28:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 01:28:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:07

Channel:Brightfield set at:12-Jul-2019 01:28:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 01:28:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:07

Channel configuration set to: GFP: 12-Jul-2019 01:28:08

Channel:GFP set at:12-Jul-2019 01:28:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 01:28:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:13

Channel:Brightfield set at:12-Jul-2019 01:28:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6501. 12-Jul-2019 01:28:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:13

Channel configuration set to: GFP: 12-Jul-2019 01:28:14

Channel:GFP set at:12-Jul-2019 01:28:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 01:28:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:18

Channel:Brightfield set at:12-Jul-2019 01:28:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 12-Jul-2019 01:28:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:18

Channel configuration set to: GFP: 12-Jul-2019 01:28:19

Channel:GFP set at:12-Jul-2019 01:28:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 12-Jul-2019 01:28:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:23

Channel:Brightfield set at:12-Jul-2019 01:28:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2501. 12-Jul-2019 01:28:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:23

Channel configuration set to: GFP: 12-Jul-2019 01:28:24

Channel:GFP set at:12-Jul-2019 01:28:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2251. 12-Jul-2019 01:28:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:28

Channel:Brightfield set at:12-Jul-2019 01:28:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4001. 12-Jul-2019 01:28:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:29

Channel configuration set to: GFP: 12-Jul-2019 01:28:30

Channel:GFP set at:12-Jul-2019 01:28:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4001. 12-Jul-2019 01:28:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:33

Channel:Brightfield set at:12-Jul-2019 01:28:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 01:28:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:33

Channel configuration set to: GFP: 12-Jul-2019 01:28:35

Channel:GFP set at:12-Jul-2019 01:28:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 01:28:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:39

Channel:Brightfield set at:12-Jul-2019 01:28:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 01:28:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:39

Channel configuration set to: GFP: 12-Jul-2019 01:28:40

Channel:GFP set at:12-Jul-2019 01:28:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 01:28:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:44

Channel:Brightfield set at:12-Jul-2019 01:28:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 01:28:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:45

Channel configuration set to: GFP: 12-Jul-2019 01:28:46

Channel:GFP set at:12-Jul-2019 01:28:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 01:28:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:49

Channel:Brightfield set at:12-Jul-2019 01:28:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 01:28:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:49

Channel configuration set to: GFP: 12-Jul-2019 01:28:51

Channel:GFP set at:12-Jul-2019 01:28:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 01:28:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.325

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:55

Channel:Brightfield set at:12-Jul-2019 01:28:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 01:28:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:55

Channel configuration set to: GFP: 12-Jul-2019 01:28:57

Channel:GFP set at:12-Jul-2019 01:28:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 01:28:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:28:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:28:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 12-Jul-2019 01:29:00

Channel:Brightfield set at:12-Jul-2019 01:29:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 01:29:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:29:00

Channel configuration set to: GFP: 12-Jul-2019 01:29:01

Channel:GFP set at:12-Jul-2019 01:29:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 01:29:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:29:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:29:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 01:29:05

Channel:Brightfield set at:12-Jul-2019 01:29:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 12-Jul-2019 01:29:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:29:05

Channel configuration set to: GFP: 12-Jul-2019 01:29:07

Channel:GFP set at:12-Jul-2019 01:29:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 12-Jul-2019 01:29:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:29:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:109 completed at:12-Jul-2019 01:29:09

Time since start of timelapse:32495.8285

PFS is locked

Time to next time point:204.8162



------Time point_110------

Memory available for all arrays: 61070938112

Memory used by Matlab: 2213076992

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:32:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.4

Channel configuration set to: Brightfield: 12-Jul-2019 01:32:35

Channel:Brightfield set at:12-Jul-2019 01:32:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 01:32:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:32:36

Channel configuration set to: GFP: 12-Jul-2019 01:32:37

Channel:GFP set at:12-Jul-2019 01:32:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 01:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:32:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:32:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.075

Channel configuration set to: Brightfield: 12-Jul-2019 01:32:41

Channel:Brightfield set at:12-Jul-2019 01:32:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 01:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:32:41

Channel configuration set to: GFP: 12-Jul-2019 01:32:42

Channel:GFP set at:12-Jul-2019 01:32:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 01:32:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:32:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:32:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.825

Channel configuration set to: Brightfield: 12-Jul-2019 01:32:46

Channel:Brightfield set at:12-Jul-2019 01:32:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 12-Jul-2019 01:32:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:32:46

Channel configuration set to: GFP: 12-Jul-2019 01:32:47

Channel:GFP set at:12-Jul-2019 01:32:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 12-Jul-2019 01:32:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:32:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:32:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.8

Channel configuration set to: Brightfield: 12-Jul-2019 01:32:51

Channel:Brightfield set at:12-Jul-2019 01:32:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 01:32:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:32:51

Channel configuration set to: GFP: 12-Jul-2019 01:32:52

Channel:GFP set at:12-Jul-2019 01:32:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 01:32:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:32:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:32:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.775

Channel configuration set to: Brightfield: 12-Jul-2019 01:32:55

Channel:Brightfield set at:12-Jul-2019 01:32:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 01:32:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:32:56

Channel configuration set to: GFP: 12-Jul-2019 01:32:57

Channel:GFP set at:12-Jul-2019 01:32:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 01:32:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:32:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:00

Channel:Brightfield set at:12-Jul-2019 01:33:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 01:33:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:01

Channel configuration set to: GFP: 12-Jul-2019 01:33:02

Channel:GFP set at:12-Jul-2019 01:33:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 01:33:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:07

Channel:Brightfield set at:12-Jul-2019 01:33:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 01:33:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:07

Channel configuration set to: GFP: 12-Jul-2019 01:33:08

Channel:GFP set at:12-Jul-2019 01:33:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 01:33:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.475

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:12

Channel:Brightfield set at:12-Jul-2019 01:33:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 12-Jul-2019 01:33:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:13

Channel configuration set to: GFP: 12-Jul-2019 01:33:14

Channel:GFP set at:12-Jul-2019 01:33:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 12-Jul-2019 01:33:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:17

Channel:Brightfield set at:12-Jul-2019 01:33:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 01:33:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:18

Channel configuration set to: GFP: 12-Jul-2019 01:33:19

Channel:GFP set at:12-Jul-2019 01:33:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 01:33:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:23

Channel:Brightfield set at:12-Jul-2019 01:33:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 01:33:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:23

Channel configuration set to: GFP: 12-Jul-2019 01:33:24

Channel:GFP set at:12-Jul-2019 01:33:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 01:33:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:28

Channel:Brightfield set at:12-Jul-2019 01:33:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 01:33:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:28

Channel configuration set to: GFP: 12-Jul-2019 01:33:29

Channel:GFP set at:12-Jul-2019 01:33:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 01:33:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:33

Channel:Brightfield set at:12-Jul-2019 01:33:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 12-Jul-2019 01:33:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:34

Channel configuration set to: GFP: 12-Jul-2019 01:33:35

Channel:GFP set at:12-Jul-2019 01:33:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 12-Jul-2019 01:33:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:39

Channel:Brightfield set at:12-Jul-2019 01:33:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 01:33:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:40

Channel configuration set to: GFP: 12-Jul-2019 01:33:41

Channel:GFP set at:12-Jul-2019 01:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 01:33:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:45

Channel:Brightfield set at:12-Jul-2019 01:33:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 01:33:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:45

Channel configuration set to: GFP: 12-Jul-2019 01:33:46

Channel:GFP set at:12-Jul-2019 01:33:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 01:33:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:49

Channel:Brightfield set at:12-Jul-2019 01:33:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 01:33:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:50

Channel configuration set to: GFP: 12-Jul-2019 01:33:51

Channel:GFP set at:12-Jul-2019 01:33:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 01:33:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:55

Channel:Brightfield set at:12-Jul-2019 01:33:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 01:33:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:55

Channel configuration set to: GFP: 12-Jul-2019 01:33:57

Channel:GFP set at:12-Jul-2019 01:33:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 12-Jul-2019 01:33:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:33:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:33:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:34:00

Channel:Brightfield set at:12-Jul-2019 01:34:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 12-Jul-2019 01:34:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:34:00

Channel configuration set to: GFP: 12-Jul-2019 01:34:02

Channel:GFP set at:12-Jul-2019 01:34:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 12-Jul-2019 01:34:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:34:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:34:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 12-Jul-2019 01:34:05

Channel:Brightfield set at:12-Jul-2019 01:34:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 01:34:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:34:05

Channel configuration set to: GFP: 12-Jul-2019 01:34:07

Channel:GFP set at:12-Jul-2019 01:34:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 01:34:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:34:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:110 completed at:12-Jul-2019 01:34:09

Time since start of timelapse:32795.5271

PFS is locked

Time to next time point:205.1191



------Time point_111------

Memory available for all arrays: 61070757888

Memory used by Matlab: 2213339136

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:37:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 12-Jul-2019 01:37:36

Channel:Brightfield set at:12-Jul-2019 01:37:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 01:37:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:37:36

Channel configuration set to: GFP: 12-Jul-2019 01:37:37

Channel:GFP set at:12-Jul-2019 01:37:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 01:37:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:37:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:37:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 01:37:41

Channel:Brightfield set at:12-Jul-2019 01:37:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 01:37:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:37:41

Channel configuration set to: GFP: 12-Jul-2019 01:37:42

Channel:GFP set at:12-Jul-2019 01:37:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6251. 12-Jul-2019 01:37:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:37:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:37:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:37:45

Channel:Brightfield set at:12-Jul-2019 01:37:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 01:37:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:37:46

Channel configuration set to: GFP: 12-Jul-2019 01:37:47

Channel:GFP set at:12-Jul-2019 01:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 01:37:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:37:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:37:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.775

Channel configuration set to: Brightfield: 12-Jul-2019 01:37:51

Channel:Brightfield set at:12-Jul-2019 01:37:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 01:37:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:37:51

Channel configuration set to: GFP: 12-Jul-2019 01:37:52

Channel:GFP set at:12-Jul-2019 01:37:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 01:37:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:37:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:37:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 12-Jul-2019 01:37:55

Channel:Brightfield set at:12-Jul-2019 01:37:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 01:37:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:37:56

Channel configuration set to: GFP: 12-Jul-2019 01:37:57

Channel:GFP set at:12-Jul-2019 01:37:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 01:37:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:37:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:37:59

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

PFS not locked. Waiting for lock...

PFS took 0.050000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:00

Channel:Brightfield set at:12-Jul-2019 01:38:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 01:38:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:01

Channel configuration set to: GFP: 12-Jul-2019 01:38:02

Channel:GFP set at:12-Jul-2019 01:38:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0001. 12-Jul-2019 01:38:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:04

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:06

Channel:Brightfield set at:12-Jul-2019 01:38:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 01:38:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:06

Channel configuration set to: GFP: 12-Jul-2019 01:38:07

Channel:GFP set at:12-Jul-2019 01:38:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 01:38:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:11

Channel:Brightfield set at:12-Jul-2019 01:38:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 12-Jul-2019 01:38:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:12

Channel configuration set to: GFP: 12-Jul-2019 01:38:13

Channel:GFP set at:12-Jul-2019 01:38:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 12-Jul-2019 01:38:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.9

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:16

Channel:Brightfield set at:12-Jul-2019 01:38:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 12-Jul-2019 01:38:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:17

Channel configuration set to: GFP: 12-Jul-2019 01:38:18

Channel:GFP set at:12-Jul-2019 01:38:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 12-Jul-2019 01:38:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:22

Channel:Brightfield set at:12-Jul-2019 01:38:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 12-Jul-2019 01:38:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:22

Channel configuration set to: GFP: 12-Jul-2019 01:38:23

Channel:GFP set at:12-Jul-2019 01:38:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 12-Jul-2019 01:38:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:27

Channel:Brightfield set at:12-Jul-2019 01:38:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 01:38:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:27

Channel configuration set to: GFP: 12-Jul-2019 01:38:29

Channel:GFP set at:12-Jul-2019 01:38:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 01:38:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:33

Channel:Brightfield set at:12-Jul-2019 01:38:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 12-Jul-2019 01:38:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:33

Channel configuration set to: GFP: 12-Jul-2019 01:38:34

Channel:GFP set at:12-Jul-2019 01:38:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 12-Jul-2019 01:38:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:38

Channel:Brightfield set at:12-Jul-2019 01:38:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 01:38:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:38

Channel configuration set to: GFP: 12-Jul-2019 01:38:39

Channel:GFP set at:12-Jul-2019 01:38:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 01:38:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:43

Channel:Brightfield set at:12-Jul-2019 01:38:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 01:38:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:44

Channel configuration set to: GFP: 12-Jul-2019 01:38:45

Channel:GFP set at:12-Jul-2019 01:38:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 01:38:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:48

Channel:Brightfield set at:12-Jul-2019 01:38:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 01:38:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:49

Channel configuration set to: GFP: 12-Jul-2019 01:38:50

Channel:GFP set at:12-Jul-2019 01:38:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 01:38:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.7

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:54

Channel:Brightfield set at:12-Jul-2019 01:38:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 01:38:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:54

Channel configuration set to: GFP: 12-Jul-2019 01:38:56

Channel:GFP set at:12-Jul-2019 01:38:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7251. 12-Jul-2019 01:38:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 01:38:58

Channel:Brightfield set at:12-Jul-2019 01:38:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 01:38:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:38:59

Channel configuration set to: GFP: 12-Jul-2019 01:39:00

Channel:GFP set at:12-Jul-2019 01:39:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 01:39:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:39:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:39:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 12-Jul-2019 01:39:04

Channel:Brightfield set at:12-Jul-2019 01:39:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 01:39:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:39:04

Channel configuration set to: GFP: 12-Jul-2019 01:39:05

Channel:GFP set at:12-Jul-2019 01:39:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 01:39:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:39:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:111 completed at:12-Jul-2019 01:39:07

Time since start of timelapse:33094.1177

PFS is locked

Time to next time point:206.5302



------Time point_112------

Memory available for all arrays: 61069295616

Memory used by Matlab: 2213715968

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:42:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.525

Channel configuration set to: Brightfield: 12-Jul-2019 01:42:36

Channel:Brightfield set at:12-Jul-2019 01:42:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8751. 12-Jul-2019 01:42:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:42:37

Channel configuration set to: GFP: 12-Jul-2019 01:42:38

Channel:GFP set at:12-Jul-2019 01:42:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 01:42:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:42:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:42:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.225

Channel configuration set to: Brightfield: 12-Jul-2019 01:42:41

Channel:Brightfield set at:12-Jul-2019 01:42:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 01:42:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:42:42

Channel configuration set to: GFP: 12-Jul-2019 01:42:43

Channel:GFP set at:12-Jul-2019 01:42:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 01:42:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:42:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:42:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.2

Channel configuration set to: Brightfield: 12-Jul-2019 01:42:46

Channel:Brightfield set at:12-Jul-2019 01:42:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 12-Jul-2019 01:42:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:42:46

Channel configuration set to: GFP: 12-Jul-2019 01:42:48

Channel:GFP set at:12-Jul-2019 01:42:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 12-Jul-2019 01:42:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:42:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:42:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 12-Jul-2019 01:42:52

Channel:Brightfield set at:12-Jul-2019 01:42:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 12-Jul-2019 01:42:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:42:52

Channel configuration set to: GFP: 12-Jul-2019 01:42:53

Channel:GFP set at:12-Jul-2019 01:42:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 12-Jul-2019 01:42:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:42:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:42:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.675

Channel configuration set to: Brightfield: 12-Jul-2019 01:42:57

Channel:Brightfield set at:12-Jul-2019 01:42:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 01:42:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:42:57

Channel configuration set to: GFP: 12-Jul-2019 01:42:59

Channel:GFP set at:12-Jul-2019 01:42:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 12-Jul-2019 01:42:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:42:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.15

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:02

Channel:Brightfield set at:12-Jul-2019 01:43:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 01:43:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:03

Channel configuration set to: GFP: 12-Jul-2019 01:43:04

Channel:GFP set at:12-Jul-2019 01:43:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 01:43:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:08

Channel:Brightfield set at:12-Jul-2019 01:43:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 01:43:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:08

Channel configuration set to: GFP: 12-Jul-2019 01:43:09

Channel:GFP set at:12-Jul-2019 01:43:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 01:43:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.65

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:13

Channel:Brightfield set at:12-Jul-2019 01:43:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 12-Jul-2019 01:43:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:14

Channel configuration set to: GFP: 12-Jul-2019 01:43:15

Channel:GFP set at:12-Jul-2019 01:43:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 12-Jul-2019 01:43:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:19

Channel:Brightfield set at:12-Jul-2019 01:43:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 01:43:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:19

Channel configuration set to: GFP: 12-Jul-2019 01:43:20

Channel:GFP set at:12-Jul-2019 01:43:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 01:43:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:24

Channel:Brightfield set at:12-Jul-2019 01:43:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 12-Jul-2019 01:43:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:24

Channel configuration set to: GFP: 12-Jul-2019 01:43:25

Channel:GFP set at:12-Jul-2019 01:43:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 12-Jul-2019 01:43:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:29

Channel:Brightfield set at:12-Jul-2019 01:43:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 01:43:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:30

Channel configuration set to: GFP: 12-Jul-2019 01:43:31

Channel:GFP set at:12-Jul-2019 01:43:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 01:43:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:34

Channel:Brightfield set at:12-Jul-2019 01:43:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 01:43:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:35

Channel configuration set to: GFP: 12-Jul-2019 01:43:36

Channel:GFP set at:12-Jul-2019 01:43:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 01:43:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:40

Channel:Brightfield set at:12-Jul-2019 01:43:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 01:43:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:40

Channel configuration set to: GFP: 12-Jul-2019 01:43:42

Channel:GFP set at:12-Jul-2019 01:43:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 01:43:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:46

Channel:Brightfield set at:12-Jul-2019 01:43:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 01:43:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:46

Channel configuration set to: GFP: 12-Jul-2019 01:43:47

Channel:GFP set at:12-Jul-2019 01:43:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 01:43:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:51

Channel:Brightfield set at:12-Jul-2019 01:43:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 01:43:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:51

Channel configuration set to: GFP: 12-Jul-2019 01:43:52

Channel:GFP set at:12-Jul-2019 01:43:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 01:43:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 12-Jul-2019 01:43:56

Channel:Brightfield set at:12-Jul-2019 01:43:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 01:43:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:57

Channel configuration set to: GFP: 12-Jul-2019 01:43:58

Channel:GFP set at:12-Jul-2019 01:43:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 01:43:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:43:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:44:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 12-Jul-2019 01:44:01

Channel:Brightfield set at:12-Jul-2019 01:44:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 01:44:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:44:01

Channel configuration set to: GFP: 12-Jul-2019 01:44:02

Channel:GFP set at:12-Jul-2019 01:44:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 01:44:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:44:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:44:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 01:44:06

Channel:Brightfield set at:12-Jul-2019 01:44:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 01:44:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:44:06

Channel configuration set to: GFP: 12-Jul-2019 01:44:08

Channel:GFP set at:12-Jul-2019 01:44:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 01:44:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:44:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:112 completed at:12-Jul-2019 01:44:10

Time since start of timelapse:33396.7195

PFS is locked

Time to next time point:203.93



------Time point_113------

Memory available for all arrays: 61064376320

Memory used by Matlab: 2213822464

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:47:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.225

Channel configuration set to: Brightfield: 12-Jul-2019 01:47:35

Channel:Brightfield set at:12-Jul-2019 01:47:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 01:47:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:47:36

Channel configuration set to: GFP: 12-Jul-2019 01:47:37

Channel:GFP set at:12-Jul-2019 01:47:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 01:47:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:47:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:47:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.95

Channel configuration set to: Brightfield: 12-Jul-2019 01:47:41

Channel:Brightfield set at:12-Jul-2019 01:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 12-Jul-2019 01:47:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:47:41

Channel configuration set to: GFP: 12-Jul-2019 01:47:42

Channel:GFP set at:12-Jul-2019 01:47:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 12-Jul-2019 01:47:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:47:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:47:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 12-Jul-2019 01:47:45

Channel:Brightfield set at:12-Jul-2019 01:47:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 12-Jul-2019 01:47:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:47:46

Channel configuration set to: GFP: 12-Jul-2019 01:47:47

Channel:GFP set at:12-Jul-2019 01:47:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9251. 12-Jul-2019 01:47:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:47:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:47:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.275

Channel configuration set to: Brightfield: 12-Jul-2019 01:47:51

Channel:Brightfield set at:12-Jul-2019 01:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 01:47:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:47:51

Channel configuration set to: GFP: 12-Jul-2019 01:47:53

Channel:GFP set at:12-Jul-2019 01:47:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 01:47:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:47:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:47:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 12-Jul-2019 01:47:56

Channel:Brightfield set at:12-Jul-2019 01:47:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 01:47:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:47:56

Channel configuration set to: GFP: 12-Jul-2019 01:47:57

Channel:GFP set at:12-Jul-2019 01:47:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 01:47:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:47:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.125

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:01

Channel:Brightfield set at:12-Jul-2019 01:48:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 01:48:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:02

Channel configuration set to: GFP: 12-Jul-2019 01:48:03

Channel:GFP set at:12-Jul-2019 01:48:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 01:48:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:07

Channel:Brightfield set at:12-Jul-2019 01:48:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 01:48:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:07

Channel configuration set to: GFP: 12-Jul-2019 01:48:08

Channel:GFP set at:12-Jul-2019 01:48:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 01:48:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:12

Channel:Brightfield set at:12-Jul-2019 01:48:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 12-Jul-2019 01:48:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:13

Channel configuration set to: GFP: 12-Jul-2019 01:48:14

Channel:GFP set at:12-Jul-2019 01:48:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 12-Jul-2019 01:48:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.625

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:17

Channel:Brightfield set at:12-Jul-2019 01:48:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 12-Jul-2019 01:48:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:18

Channel configuration set to: GFP: 12-Jul-2019 01:48:19

Channel:GFP set at:12-Jul-2019 01:48:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 01:48:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:23

Channel:Brightfield set at:12-Jul-2019 01:48:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 12-Jul-2019 01:48:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:23

Channel configuration set to: GFP: 12-Jul-2019 01:48:25

Channel:GFP set at:12-Jul-2019 01:48:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 12-Jul-2019 01:48:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:28

Channel:Brightfield set at:12-Jul-2019 01:48:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 01:48:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:28

Channel configuration set to: GFP: 12-Jul-2019 01:48:30

Channel:GFP set at:12-Jul-2019 01:48:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 01:48:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:34

Channel:Brightfield set at:12-Jul-2019 01:48:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 12-Jul-2019 01:48:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:34

Channel configuration set to: GFP: 12-Jul-2019 01:48:35

Channel:GFP set at:12-Jul-2019 01:48:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 12-Jul-2019 01:48:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:39

Channel:Brightfield set at:12-Jul-2019 01:48:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 01:48:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:39

Channel configuration set to: GFP: 12-Jul-2019 01:48:41

Channel:GFP set at:12-Jul-2019 01:48:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 01:48:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:44

Channel:Brightfield set at:12-Jul-2019 01:48:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 01:48:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:44

Channel configuration set to: GFP: 12-Jul-2019 01:48:46

Channel:GFP set at:12-Jul-2019 01:48:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 01:48:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:49

Channel:Brightfield set at:12-Jul-2019 01:48:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 01:48:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:50

Channel configuration set to: GFP: 12-Jul-2019 01:48:51

Channel:GFP set at:12-Jul-2019 01:48:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 01:48:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.4

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:55

Channel:Brightfield set at:12-Jul-2019 01:48:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 12-Jul-2019 01:48:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:55

Channel configuration set to: GFP: 12-Jul-2019 01:48:56

Channel:GFP set at:12-Jul-2019 01:48:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 01:48:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:48:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:48:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.925

PFS not locked. Waiting for lock...

PFS took 0.450000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:49:00

Channel:Brightfield set at:12-Jul-2019 01:49:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 01:49:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:49:00

Channel configuration set to: GFP: 12-Jul-2019 01:49:01

Channel:GFP set at:12-Jul-2019 01:49:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 01:49:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:49:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:49:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 01:49:06

Channel:Brightfield set at:12-Jul-2019 01:49:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6751. 12-Jul-2019 01:49:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:49:06

Channel configuration set to: GFP: 12-Jul-2019 01:49:07

Channel:GFP set at:12-Jul-2019 01:49:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6501. 12-Jul-2019 01:49:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:49:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:113 completed at:12-Jul-2019 01:49:10

Time since start of timelapse:33696.674

PFS is locked

Time to next time point:203.9772



------Time point_114------

Memory available for all arrays: 61087645696

Memory used by Matlab: 2214174720

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:52:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.275

Channel configuration set to: Brightfield: 12-Jul-2019 01:52:35

Channel:Brightfield set at:12-Jul-2019 01:52:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 01:52:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:52:36

Channel configuration set to: GFP: 12-Jul-2019 01:52:37

Channel:GFP set at:12-Jul-2019 01:52:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 01:52:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:52:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:52:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.9

Channel configuration set to: Brightfield: 12-Jul-2019 01:52:41

Channel:Brightfield set at:12-Jul-2019 01:52:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 12-Jul-2019 01:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:52:41

Channel configuration set to: GFP: 12-Jul-2019 01:52:42

Channel:GFP set at:12-Jul-2019 01:52:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 12-Jul-2019 01:52:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:52:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:52:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.775

Channel configuration set to: Brightfield: 12-Jul-2019 01:52:46

Channel:Brightfield set at:12-Jul-2019 01:52:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1751. 12-Jul-2019 01:52:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:52:46

Channel configuration set to: GFP: 12-Jul-2019 01:52:47

Channel:GFP set at:12-Jul-2019 01:52:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1751. 12-Jul-2019 01:52:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:52:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:52:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 12-Jul-2019 01:52:51

Channel:Brightfield set at:12-Jul-2019 01:52:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3001. 12-Jul-2019 01:52:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:52:51

Channel configuration set to: GFP: 12-Jul-2019 01:52:53

Channel:GFP set at:12-Jul-2019 01:52:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3001. 12-Jul-2019 01:52:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:52:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:52:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.675

Channel configuration set to: Brightfield: 12-Jul-2019 01:52:56

Channel:Brightfield set at:12-Jul-2019 01:52:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 01:52:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:52:57

Channel configuration set to: GFP: 12-Jul-2019 01:52:58

Channel:GFP set at:12-Jul-2019 01:52:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 01:52:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:52:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:01

Channel:Brightfield set at:12-Jul-2019 01:53:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 12-Jul-2019 01:53:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:02

Channel configuration set to: GFP: 12-Jul-2019 01:53:03

Channel:GFP set at:12-Jul-2019 01:53:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 12-Jul-2019 01:53:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:07

Channel:Brightfield set at:12-Jul-2019 01:53:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 01:53:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:08

Channel configuration set to: GFP: 12-Jul-2019 01:53:09

Channel:GFP set at:12-Jul-2019 01:53:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 01:53:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:12

Channel:Brightfield set at:12-Jul-2019 01:53:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 01:53:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:12

Channel configuration set to: GFP: 12-Jul-2019 01:53:14

Channel:GFP set at:12-Jul-2019 01:53:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 01:53:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:16

Channel:Brightfield set at:12-Jul-2019 01:53:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 01:53:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:17

Channel configuration set to: GFP: 12-Jul-2019 01:53:18

Channel:GFP set at:12-Jul-2019 01:53:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 01:53:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:22

Channel:Brightfield set at:12-Jul-2019 01:53:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 12-Jul-2019 01:53:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:22

Channel configuration set to: GFP: 12-Jul-2019 01:53:23

Channel:GFP set at:12-Jul-2019 01:53:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 12-Jul-2019 01:53:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:27

Channel:Brightfield set at:12-Jul-2019 01:53:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 01:53:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:27

Channel configuration set to: GFP: 12-Jul-2019 01:53:29

Channel:GFP set at:12-Jul-2019 01:53:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 01:53:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:32

Channel:Brightfield set at:12-Jul-2019 01:53:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 01:53:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:32

Channel configuration set to: GFP: 12-Jul-2019 01:53:34

Channel:GFP set at:12-Jul-2019 01:53:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 01:53:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:38

Channel:Brightfield set at:12-Jul-2019 01:53:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 01:53:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:38

Channel configuration set to: GFP: 12-Jul-2019 01:53:39

Channel:GFP set at:12-Jul-2019 01:53:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 01:53:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:42

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:43

Channel:Brightfield set at:12-Jul-2019 01:53:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 01:53:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:44

Channel configuration set to: GFP: 12-Jul-2019 01:53:45

Channel:GFP set at:12-Jul-2019 01:53:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 01:53:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.45

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:49

Channel:Brightfield set at:12-Jul-2019 01:53:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 01:53:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:49

Channel configuration set to: GFP: 12-Jul-2019 01:53:50

Channel:GFP set at:12-Jul-2019 01:53:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 01:53:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:55

Channel:Brightfield set at:12-Jul-2019 01:53:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 12-Jul-2019 01:53:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:55

Channel configuration set to: GFP: 12-Jul-2019 01:53:56

Channel:GFP set at:12-Jul-2019 01:53:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 12-Jul-2019 01:53:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:53:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:53:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.15

Channel configuration set to: Brightfield: 12-Jul-2019 01:54:00

Channel:Brightfield set at:12-Jul-2019 01:54:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 01:54:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:54:00

Channel configuration set to: GFP: 12-Jul-2019 01:54:02

Channel:GFP set at:12-Jul-2019 01:54:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 01:54:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:54:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:54:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 12-Jul-2019 01:54:05

Channel:Brightfield set at:12-Jul-2019 01:54:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5501. 12-Jul-2019 01:54:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:54:06

Channel configuration set to: GFP: 12-Jul-2019 01:54:07

Channel:GFP set at:12-Jul-2019 01:54:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5501. 12-Jul-2019 01:54:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:54:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:114 completed at:12-Jul-2019 01:54:09

Time since start of timelapse:33996.15

PFS is locked

Time to next time point:204.5029



------Time point_115------

Memory available for all arrays: 61087956992

Memory used by Matlab: 2214174720

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:57:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-11.8

Channel configuration set to: Brightfield: 12-Jul-2019 01:57:35

Channel:Brightfield set at:12-Jul-2019 01:57:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 01:57:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:57:35

Channel configuration set to: GFP: 12-Jul-2019 01:57:37

Channel:GFP set at:12-Jul-2019 01:57:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 01:57:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:57:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:57:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9

Channel configuration set to: Brightfield: 12-Jul-2019 01:57:41

Channel:Brightfield set at:12-Jul-2019 01:57:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 12-Jul-2019 01:57:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:57:42

Channel configuration set to: GFP: 12-Jul-2019 01:57:43

Channel:GFP set at:12-Jul-2019 01:57:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8751. 12-Jul-2019 01:57:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:57:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:57:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 12-Jul-2019 01:57:46

Channel:Brightfield set at:12-Jul-2019 01:57:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 12-Jul-2019 01:57:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:57:46

Channel configuration set to: GFP: 12-Jul-2019 01:57:48

Channel:GFP set at:12-Jul-2019 01:57:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 12-Jul-2019 01:57:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:57:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:57:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.675

Channel configuration set to: Brightfield: 12-Jul-2019 01:57:51

Channel:Brightfield set at:12-Jul-2019 01:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 12-Jul-2019 01:57:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:57:51

Channel configuration set to: GFP: 12-Jul-2019 01:57:53

Channel:GFP set at:12-Jul-2019 01:57:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 12-Jul-2019 01:57:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:57:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:57:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.65

Channel configuration set to: Brightfield: 12-Jul-2019 01:57:56

Channel:Brightfield set at:12-Jul-2019 01:57:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 01:57:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:57:57

Channel configuration set to: GFP: 12-Jul-2019 01:57:58

Channel:GFP set at:12-Jul-2019 01:57:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 01:57:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:57:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.05

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:02

Channel:Brightfield set at:12-Jul-2019 01:58:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 01:58:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:03

Channel configuration set to: GFP: 12-Jul-2019 01:58:04

Channel:GFP set at:12-Jul-2019 01:58:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 01:58:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:07

Channel:Brightfield set at:12-Jul-2019 01:58:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 01:58:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:08

Channel configuration set to: GFP: 12-Jul-2019 01:58:09

Channel:GFP set at:12-Jul-2019 01:58:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 01:58:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:13

Channel:Brightfield set at:12-Jul-2019 01:58:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 12-Jul-2019 01:58:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:13

Channel configuration set to: GFP: 12-Jul-2019 01:58:15

Channel:GFP set at:12-Jul-2019 01:58:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 12-Jul-2019 01:58:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:19

Channel:Brightfield set at:12-Jul-2019 01:58:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 01:58:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:19

Channel configuration set to: GFP: 12-Jul-2019 01:58:20

Channel:GFP set at:12-Jul-2019 01:58:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 01:58:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:23

Channel:Brightfield set at:12-Jul-2019 01:58:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 01:58:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:24

Channel configuration set to: GFP: 12-Jul-2019 01:58:25

Channel:GFP set at:12-Jul-2019 01:58:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 01:58:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:29

Channel:Brightfield set at:12-Jul-2019 01:58:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 01:58:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:29

Channel configuration set to: GFP: 12-Jul-2019 01:58:30

Channel:GFP set at:12-Jul-2019 01:58:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 01:58:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:34

Channel:Brightfield set at:12-Jul-2019 01:58:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 01:58:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:34

Channel configuration set to: GFP: 12-Jul-2019 01:58:35

Channel:GFP set at:12-Jul-2019 01:58:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 01:58:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:39

Channel:Brightfield set at:12-Jul-2019 01:58:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 01:58:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:39

Channel configuration set to: GFP: 12-Jul-2019 01:58:41

Channel:GFP set at:12-Jul-2019 01:58:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 01:58:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:45

Channel:Brightfield set at:12-Jul-2019 01:58:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 01:58:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:45

Channel configuration set to: GFP: 12-Jul-2019 01:58:46

Channel:GFP set at:12-Jul-2019 01:58:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 01:58:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:49

Channel:Brightfield set at:12-Jul-2019 01:58:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 01:58:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:50

Channel configuration set to: GFP: 12-Jul-2019 01:58:51

Channel:GFP set at:12-Jul-2019 01:58:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 01:58:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:55

Channel:Brightfield set at:12-Jul-2019 01:58:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 01:58:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:55

Channel configuration set to: GFP: 12-Jul-2019 01:58:57

Channel:GFP set at:12-Jul-2019 01:58:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 12-Jul-2019 01:58:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:58:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:58:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.975

PFS not locked. Waiting for lock...

PFS took 0.400000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 01:59:00

Channel:Brightfield set at:12-Jul-2019 01:59:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 01:59:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:59:00

Channel configuration set to: GFP: 12-Jul-2019 01:59:01

Channel:GFP set at:12-Jul-2019 01:59:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 01:59:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:59:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 01:59:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 01:59:05

Channel:Brightfield set at:12-Jul-2019 01:59:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 12-Jul-2019 01:59:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:59:06

Channel configuration set to: GFP: 12-Jul-2019 01:59:07

Channel:GFP set at:12-Jul-2019 01:59:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3751. 12-Jul-2019 01:59:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 01:59:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:115 completed at:12-Jul-2019 01:59:09

Time since start of timelapse:34295.9055

PFS is locked

Time to next time point:204.7491



------Time point_116------

Memory available for all arrays: 61084725248

Memory used by Matlab: 2214699008

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:02:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 12-Jul-2019 02:02:35

Channel:Brightfield set at:12-Jul-2019 02:02:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 12-Jul-2019 02:02:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:02:36

Channel configuration set to: GFP: 12-Jul-2019 02:02:37

Channel:GFP set at:12-Jul-2019 02:02:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 12-Jul-2019 02:02:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:02:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:02:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.875

Channel configuration set to: Brightfield: 12-Jul-2019 02:02:41

Channel:Brightfield set at:12-Jul-2019 02:02:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9751. 12-Jul-2019 02:02:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:02:41

Channel configuration set to: GFP: 12-Jul-2019 02:02:42

Channel:GFP set at:12-Jul-2019 02:02:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 02:02:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:02:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:02:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 12-Jul-2019 02:02:45

Channel:Brightfield set at:12-Jul-2019 02:02:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8751. 12-Jul-2019 02:02:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:02:46

Channel configuration set to: GFP: 12-Jul-2019 02:02:47

Channel:GFP set at:12-Jul-2019 02:02:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8501. 12-Jul-2019 02:02:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:02:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:02:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.575

Channel configuration set to: Brightfield: 12-Jul-2019 02:02:51

Channel:Brightfield set at:12-Jul-2019 02:02:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 12-Jul-2019 02:02:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:02:51

Channel configuration set to: GFP: 12-Jul-2019 02:02:52

Channel:GFP set at:12-Jul-2019 02:02:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 02:02:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:02:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:02:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.525

Channel configuration set to: Brightfield: 12-Jul-2019 02:02:55

Channel:Brightfield set at:12-Jul-2019 02:02:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 12-Jul-2019 02:02:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:02:56

Channel configuration set to: GFP: 12-Jul-2019 02:02:57

Channel:GFP set at:12-Jul-2019 02:02:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 12-Jul-2019 02:02:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:02:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:01

Channel:Brightfield set at:12-Jul-2019 02:03:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 02:03:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:01

Channel configuration set to: GFP: 12-Jul-2019 02:03:03

Channel:GFP set at:12-Jul-2019 02:03:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 02:03:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:06

Channel:Brightfield set at:12-Jul-2019 02:03:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 02:03:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:06

Channel configuration set to: GFP: 12-Jul-2019 02:03:07

Channel:GFP set at:12-Jul-2019 02:03:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 02:03:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:11

Channel:Brightfield set at:12-Jul-2019 02:03:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 02:03:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:11

Channel configuration set to: GFP: 12-Jul-2019 02:03:13

Channel:GFP set at:12-Jul-2019 02:03:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 02:03:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:16

Channel:Brightfield set at:12-Jul-2019 02:03:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 12-Jul-2019 02:03:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:16

Channel configuration set to: GFP: 12-Jul-2019 02:03:18

Channel:GFP set at:12-Jul-2019 02:03:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 02:03:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:21

Channel:Brightfield set at:12-Jul-2019 02:03:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 02:03:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:22

Channel configuration set to: GFP: 12-Jul-2019 02:03:23

Channel:GFP set at:12-Jul-2019 02:03:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 02:03:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:27

Channel:Brightfield set at:12-Jul-2019 02:03:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 02:03:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:27

Channel configuration set to: GFP: 12-Jul-2019 02:03:28

Channel:GFP set at:12-Jul-2019 02:03:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 02:03:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:32

Channel:Brightfield set at:12-Jul-2019 02:03:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 02:03:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:32

Channel configuration set to: GFP: 12-Jul-2019 02:03:34

Channel:GFP set at:12-Jul-2019 02:03:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 02:03:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:37

Channel:Brightfield set at:12-Jul-2019 02:03:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 02:03:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:38

Channel configuration set to: GFP: 12-Jul-2019 02:03:39

Channel:GFP set at:12-Jul-2019 02:03:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 02:03:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:42

Channel:Brightfield set at:12-Jul-2019 02:03:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:03:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:42

Channel configuration set to: GFP: 12-Jul-2019 02:03:43

Channel:GFP set at:12-Jul-2019 02:03:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:03:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.525

PFS not locked. Waiting for lock...

PFS took 0.300000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:47

Channel:Brightfield set at:12-Jul-2019 02:03:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 02:03:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:47

Channel configuration set to: GFP: 12-Jul-2019 02:03:49

Channel:GFP set at:12-Jul-2019 02:03:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 02:03:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.4

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:53

Channel:Brightfield set at:12-Jul-2019 02:03:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 02:03:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:53

Channel configuration set to: GFP: 12-Jul-2019 02:03:55

Channel:GFP set at:12-Jul-2019 02:03:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 02:03:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.075

Channel configuration set to: Brightfield: 12-Jul-2019 02:03:58

Channel:Brightfield set at:12-Jul-2019 02:03:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 12-Jul-2019 02:03:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:03:58

Channel configuration set to: GFP: 12-Jul-2019 02:04:00

Channel:GFP set at:12-Jul-2019 02:04:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 12-Jul-2019 02:04:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:04:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:04:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 02:04:03

Channel:Brightfield set at:12-Jul-2019 02:04:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 02:04:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:04:03

Channel configuration set to: GFP: 12-Jul-2019 02:04:05

Channel:GFP set at:12-Jul-2019 02:04:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 12-Jul-2019 02:04:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:04:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:116 completed at:12-Jul-2019 02:04:07

Time since start of timelapse:34593.8513

PFS is locked

Time to next time point:206.805



------Time point_117------

Memory available for all arrays: 61085487104

Memory used by Matlab: 2214699008

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:07:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.55

Channel configuration set to: Brightfield: 12-Jul-2019 02:07:36

Channel:Brightfield set at:12-Jul-2019 02:07:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 02:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:07:36

Channel configuration set to: GFP: 12-Jul-2019 02:07:37

Channel:GFP set at:12-Jul-2019 02:07:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 02:07:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:07:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:07:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.85

Channel configuration set to: Brightfield: 12-Jul-2019 02:07:41

Channel:Brightfield set at:12-Jul-2019 02:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 02:07:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:07:41

Channel configuration set to: GFP: 12-Jul-2019 02:07:43

Channel:GFP set at:12-Jul-2019 02:07:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 02:07:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:07:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:07:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 12-Jul-2019 02:07:46

Channel:Brightfield set at:12-Jul-2019 02:07:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 12-Jul-2019 02:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:07:46

Channel configuration set to: GFP: 12-Jul-2019 02:07:48

Channel:GFP set at:12-Jul-2019 02:07:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 12-Jul-2019 02:07:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:07:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:07:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.5

Channel configuration set to: Brightfield: 12-Jul-2019 02:07:51

Channel:Brightfield set at:12-Jul-2019 02:07:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 12-Jul-2019 02:07:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:07:52

Channel configuration set to: GFP: 12-Jul-2019 02:07:53

Channel:GFP set at:12-Jul-2019 02:07:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 02:07:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:07:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:07:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.5

Channel configuration set to: Brightfield: 12-Jul-2019 02:07:57

Channel:Brightfield set at:12-Jul-2019 02:07:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 02:07:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:07:57

Channel configuration set to: GFP: 12-Jul-2019 02:07:58

Channel:GFP set at:12-Jul-2019 02:07:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 02:07:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:07:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:02

Channel:Brightfield set at:12-Jul-2019 02:08:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 02:08:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:03

Channel configuration set to: GFP: 12-Jul-2019 02:08:04

Channel:GFP set at:12-Jul-2019 02:08:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 02:08:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:08

Channel:Brightfield set at:12-Jul-2019 02:08:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 02:08:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:08

Channel configuration set to: GFP: 12-Jul-2019 02:08:10

Channel:GFP set at:12-Jul-2019 02:08:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 12-Jul-2019 02:08:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:14

Channel:Brightfield set at:12-Jul-2019 02:08:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 02:08:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:14

Channel configuration set to: GFP: 12-Jul-2019 02:08:15

Channel:GFP set at:12-Jul-2019 02:08:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 02:08:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.625

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:19

Channel:Brightfield set at:12-Jul-2019 02:08:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 02:08:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:19

Channel configuration set to: GFP: 12-Jul-2019 02:08:20

Channel:GFP set at:12-Jul-2019 02:08:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 02:08:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:24

Channel:Brightfield set at:12-Jul-2019 02:08:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 02:08:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:25

Channel configuration set to: GFP: 12-Jul-2019 02:08:26

Channel:GFP set at:12-Jul-2019 02:08:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 12-Jul-2019 02:08:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:29

Channel:Brightfield set at:12-Jul-2019 02:08:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 02:08:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:30

Channel configuration set to: GFP: 12-Jul-2019 02:08:31

Channel:GFP set at:12-Jul-2019 02:08:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 02:08:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:35

Channel:Brightfield set at:12-Jul-2019 02:08:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 02:08:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:36

Channel configuration set to: GFP: 12-Jul-2019 02:08:37

Channel:GFP set at:12-Jul-2019 02:08:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 02:08:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:41

Channel:Brightfield set at:12-Jul-2019 02:08:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 02:08:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:41

Channel configuration set to: GFP: 12-Jul-2019 02:08:42

Channel:GFP set at:12-Jul-2019 02:08:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 02:08:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.1

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:46

Channel:Brightfield set at:12-Jul-2019 02:08:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 02:08:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:47

Channel configuration set to: GFP: 12-Jul-2019 02:08:48

Channel:GFP set at:12-Jul-2019 02:08:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 02:08:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:52

Channel:Brightfield set at:12-Jul-2019 02:08:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 02:08:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:52

Channel configuration set to: GFP: 12-Jul-2019 02:08:53

Channel:GFP set at:12-Jul-2019 02:08:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 02:08:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:54

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.2

Channel configuration set to: Brightfield: 12-Jul-2019 02:08:57

Channel:Brightfield set at:12-Jul-2019 02:08:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 12-Jul-2019 02:08:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:58

Channel configuration set to: GFP: 12-Jul-2019 02:08:59

Channel:GFP set at:12-Jul-2019 02:08:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2001. 12-Jul-2019 02:08:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:08:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:09:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.675

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:09:03

Channel:Brightfield set at:12-Jul-2019 02:09:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 12-Jul-2019 02:09:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:09:03

Channel configuration set to: GFP: 12-Jul-2019 02:09:04

Channel:GFP set at:12-Jul-2019 02:09:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 12-Jul-2019 02:09:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:09:05

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:09:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 12-Jul-2019 02:09:08

Channel:Brightfield set at:12-Jul-2019 02:09:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 12-Jul-2019 02:09:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:09:08

Channel configuration set to: GFP: 12-Jul-2019 02:09:09

Channel:GFP set at:12-Jul-2019 02:09:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 12-Jul-2019 02:09:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:09:10

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:117 completed at:12-Jul-2019 02:09:12

Time since start of timelapse:34898.548

PFS is locked

Time to next time point:202.1095



------Time point_118------

Memory available for all arrays: 61060493312

Memory used by Matlab: 2214699008

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:12:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 02:12:36

Channel:Brightfield set at:12-Jul-2019 02:12:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 02:12:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:12:36

Channel configuration set to: GFP: 12-Jul-2019 02:12:38

Channel:GFP set at:12-Jul-2019 02:12:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 02:12:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:12:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:12:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.5

Channel configuration set to: Brightfield: 12-Jul-2019 02:12:41

Channel:Brightfield set at:12-Jul-2019 02:12:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 12-Jul-2019 02:12:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:12:41

Channel configuration set to: GFP: 12-Jul-2019 02:12:42

Channel:GFP set at:12-Jul-2019 02:12:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 12-Jul-2019 02:12:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:12:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:12:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.675

Channel configuration set to: Brightfield: 12-Jul-2019 02:12:46

Channel:Brightfield set at:12-Jul-2019 02:12:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 12-Jul-2019 02:12:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:12:47

Channel configuration set to: GFP: 12-Jul-2019 02:12:48

Channel:GFP set at:12-Jul-2019 02:12:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 12-Jul-2019 02:12:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:12:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:12:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 12-Jul-2019 02:12:51

Channel:Brightfield set at:12-Jul-2019 02:12:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 02:12:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:12:52

Channel configuration set to: GFP: 12-Jul-2019 02:12:53

Channel:GFP set at:12-Jul-2019 02:12:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 02:12:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:12:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:12:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 12-Jul-2019 02:12:57

Channel:Brightfield set at:12-Jul-2019 02:12:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 02:12:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:12:57

Channel configuration set to: GFP: 12-Jul-2019 02:12:58

Channel:GFP set at:12-Jul-2019 02:12:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 02:12:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:12:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-7.925

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:02

Channel:Brightfield set at:12-Jul-2019 02:13:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 02:13:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:02

Channel configuration set to: GFP: 12-Jul-2019 02:13:04

Channel:GFP set at:12-Jul-2019 02:13:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 02:13:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:07

Channel:Brightfield set at:12-Jul-2019 02:13:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 02:13:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:07

Channel configuration set to: GFP: 12-Jul-2019 02:13:09

Channel:GFP set at:12-Jul-2019 02:13:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 02:13:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:12

Channel:Brightfield set at:12-Jul-2019 02:13:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 02:13:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:12

Channel configuration set to: GFP: 12-Jul-2019 02:13:13

Channel:GFP set at:12-Jul-2019 02:13:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 02:13:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.85

PFS not locked. Waiting for lock...

PFS took 0.050000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:16

Channel:Brightfield set at:12-Jul-2019 02:13:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0001. 12-Jul-2019 02:13:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:17

Channel configuration set to: GFP: 12-Jul-2019 02:13:18

Channel:GFP set at:12-Jul-2019 02:13:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 02:13:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:22

Channel:Brightfield set at:12-Jul-2019 02:13:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 02:13:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:22

Channel configuration set to: GFP: 12-Jul-2019 02:13:24

Channel:GFP set at:12-Jul-2019 02:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 02:13:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:27

Channel:Brightfield set at:12-Jul-2019 02:13:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 12-Jul-2019 02:13:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:28

Channel configuration set to: GFP: 12-Jul-2019 02:13:29

Channel:GFP set at:12-Jul-2019 02:13:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 12-Jul-2019 02:13:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:32

Channel:Brightfield set at:12-Jul-2019 02:13:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2251. 12-Jul-2019 02:13:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:33

Channel configuration set to: GFP: 12-Jul-2019 02:13:34

Channel:GFP set at:12-Jul-2019 02:13:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2251. 12-Jul-2019 02:13:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:38

Channel:Brightfield set at:12-Jul-2019 02:13:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 02:13:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:39

Channel configuration set to: GFP: 12-Jul-2019 02:13:40

Channel:GFP set at:12-Jul-2019 02:13:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 02:13:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:43

Channel:Brightfield set at:12-Jul-2019 02:13:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 02:13:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:44

Channel configuration set to: GFP: 12-Jul-2019 02:13:45

Channel:GFP set at:12-Jul-2019 02:13:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 02:13:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:45

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:48

Channel:Brightfield set at:12-Jul-2019 02:13:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 02:13:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:48

Channel configuration set to: GFP: 12-Jul-2019 02:13:50

Channel:GFP set at:12-Jul-2019 02:13:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 02:13:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:54

Channel:Brightfield set at:12-Jul-2019 02:13:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 12-Jul-2019 02:13:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:54

Channel configuration set to: GFP: 12-Jul-2019 02:13:55

Channel:GFP set at:12-Jul-2019 02:13:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 12-Jul-2019 02:13:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.15

Channel configuration set to: Brightfield: 12-Jul-2019 02:13:59

Channel:Brightfield set at:12-Jul-2019 02:13:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 02:13:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:13:59

Channel configuration set to: GFP: 12-Jul-2019 02:14:00

Channel:GFP set at:12-Jul-2019 02:14:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 02:14:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:14:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:14:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 12-Jul-2019 02:14:04

Channel:Brightfield set at:12-Jul-2019 02:14:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 02:14:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:14:05

Channel configuration set to: GFP: 12-Jul-2019 02:14:06

Channel:GFP set at:12-Jul-2019 02:14:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 02:14:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:14:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:118 completed at:12-Jul-2019 02:14:08

Time since start of timelapse:35195.2055

PFS is locked

Time to next time point:205.4532



------Time point_119------

Memory available for all arrays: 61074964480

Memory used by Matlab: 2214699008

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:17:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 12-Jul-2019 02:17:36

Channel:Brightfield set at:12-Jul-2019 02:17:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 02:17:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:17:36

Channel configuration set to: GFP: 12-Jul-2019 02:17:38

Channel:GFP set at:12-Jul-2019 02:17:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 12-Jul-2019 02:17:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:17:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:17:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.8

Channel configuration set to: Brightfield: 12-Jul-2019 02:17:41

Channel:Brightfield set at:12-Jul-2019 02:17:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0751. 12-Jul-2019 02:17:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:17:42

Channel configuration set to: GFP: 12-Jul-2019 02:17:43

Channel:GFP set at:12-Jul-2019 02:17:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0751. 12-Jul-2019 02:17:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:17:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:17:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 12-Jul-2019 02:17:46

Channel:Brightfield set at:12-Jul-2019 02:17:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 12-Jul-2019 02:17:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:17:46

Channel configuration set to: GFP: 12-Jul-2019 02:17:47

Channel:GFP set at:12-Jul-2019 02:17:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9501. 12-Jul-2019 02:17:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:17:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:17:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.575

Channel configuration set to: Brightfield: 12-Jul-2019 02:17:51

Channel:Brightfield set at:12-Jul-2019 02:17:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 12-Jul-2019 02:17:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:17:51

Channel configuration set to: GFP: 12-Jul-2019 02:17:53

Channel:GFP set at:12-Jul-2019 02:17:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 02:17:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:17:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:17:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 12-Jul-2019 02:17:56

Channel:Brightfield set at:12-Jul-2019 02:17:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 02:17:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:17:57

Channel configuration set to: GFP: 12-Jul-2019 02:17:58

Channel:GFP set at:12-Jul-2019 02:17:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 02:17:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:17:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:01

Channel:Brightfield set at:12-Jul-2019 02:18:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 02:18:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:02

Channel configuration set to: GFP: 12-Jul-2019 02:18:03

Channel:GFP set at:12-Jul-2019 02:18:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 02:18:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:07

Channel:Brightfield set at:12-Jul-2019 02:18:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 02:18:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:08

Channel configuration set to: GFP: 12-Jul-2019 02:18:09

Channel:GFP set at:12-Jul-2019 02:18:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 02:18:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:13

Channel:Brightfield set at:12-Jul-2019 02:18:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6001. 12-Jul-2019 02:18:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:13

Channel configuration set to: GFP: 12-Jul-2019 02:18:14

Channel:GFP set at:12-Jul-2019 02:18:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 02:18:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:18

Channel:Brightfield set at:12-Jul-2019 02:18:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 02:18:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:19

Channel configuration set to: GFP: 12-Jul-2019 02:18:20

Channel:GFP set at:12-Jul-2019 02:18:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 02:18:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:23

Channel:Brightfield set at:12-Jul-2019 02:18:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 02:18:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:24

Channel configuration set to: GFP: 12-Jul-2019 02:18:25

Channel:GFP set at:12-Jul-2019 02:18:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 02:18:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:29

Channel:Brightfield set at:12-Jul-2019 02:18:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5001. 12-Jul-2019 02:18:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:29

Channel configuration set to: GFP: 12-Jul-2019 02:18:31

Channel:GFP set at:12-Jul-2019 02:18:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5001. 12-Jul-2019 02:18:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:34

Channel:Brightfield set at:12-Jul-2019 02:18:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 02:18:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:34

Channel configuration set to: GFP: 12-Jul-2019 02:18:35

Channel:GFP set at:12-Jul-2019 02:18:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 02:18:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:40

Channel:Brightfield set at:12-Jul-2019 02:18:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 02:18:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:40

Channel configuration set to: GFP: 12-Jul-2019 02:18:41

Channel:GFP set at:12-Jul-2019 02:18:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 02:18:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:45

Channel:Brightfield set at:12-Jul-2019 02:18:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7001. 12-Jul-2019 02:18:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:46

Channel configuration set to: GFP: 12-Jul-2019 02:18:47

Channel:GFP set at:12-Jul-2019 02:18:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7001. 12-Jul-2019 02:18:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.475

PFS not locked. Waiting for lock...

PFS took 0.250000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:51

Channel:Brightfield set at:12-Jul-2019 02:18:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 02:18:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:51

Channel configuration set to: GFP: 12-Jul-2019 02:18:53

Channel:GFP set at:12-Jul-2019 02:18:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 02:18:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 12-Jul-2019 02:18:56

Channel:Brightfield set at:12-Jul-2019 02:18:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 12-Jul-2019 02:18:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:57

Channel configuration set to: GFP: 12-Jul-2019 02:18:58

Channel:GFP set at:12-Jul-2019 02:18:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 12-Jul-2019 02:18:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:18:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:19:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 12-Jul-2019 02:19:01

Channel:Brightfield set at:12-Jul-2019 02:19:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 12-Jul-2019 02:19:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:19:01

Channel configuration set to: GFP: 12-Jul-2019 02:19:03

Channel:GFP set at:12-Jul-2019 02:19:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 12-Jul-2019 02:19:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:19:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:19:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 12-Jul-2019 02:19:07

Channel:Brightfield set at:12-Jul-2019 02:19:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 02:19:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:19:07

Channel configuration set to: GFP: 12-Jul-2019 02:19:08

Channel:GFP set at:12-Jul-2019 02:19:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 02:19:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:19:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:119 completed at:12-Jul-2019 02:19:11

Time since start of timelapse:35497.5293

PFS is locked

Time to next time point:203.1308



------Time point_120------

Memory available for all arrays: 61079293952

Memory used by Matlab: 2214699008

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:22:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9

Channel configuration set to: Brightfield: 12-Jul-2019 02:22:35

Channel:Brightfield set at:12-Jul-2019 02:22:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 02:22:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:22:36

Channel configuration set to: GFP: 12-Jul-2019 02:22:37

Channel:GFP set at:12-Jul-2019 02:22:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3251. 12-Jul-2019 02:22:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:22:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:22:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.7

Channel configuration set to: Brightfield: 12-Jul-2019 02:22:41

Channel:Brightfield set at:12-Jul-2019 02:22:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 12-Jul-2019 02:22:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:22:41

Channel configuration set to: GFP: 12-Jul-2019 02:22:42

Channel:GFP set at:12-Jul-2019 02:22:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 12-Jul-2019 02:22:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:22:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:22:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.9

Channel configuration set to: Brightfield: 12-Jul-2019 02:22:45

Channel:Brightfield set at:12-Jul-2019 02:22:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 12-Jul-2019 02:22:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:22:46

Channel configuration set to: GFP: 12-Jul-2019 02:22:47

Channel:GFP set at:12-Jul-2019 02:22:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9751. 12-Jul-2019 02:22:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:22:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:22:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.625

Channel configuration set to: Brightfield: 12-Jul-2019 02:22:51

Channel:Brightfield set at:12-Jul-2019 02:22:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 02:22:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:22:51

Channel configuration set to: GFP: 12-Jul-2019 02:22:53

Channel:GFP set at:12-Jul-2019 02:22:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 02:22:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:22:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:22:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 12-Jul-2019 02:22:56

Channel:Brightfield set at:12-Jul-2019 02:22:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 02:22:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:22:57

Channel configuration set to: GFP: 12-Jul-2019 02:22:58

Channel:GFP set at:12-Jul-2019 02:22:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 02:22:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:22:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:01

Channel:Brightfield set at:12-Jul-2019 02:23:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3501. 12-Jul-2019 02:23:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:02

Channel configuration set to: GFP: 12-Jul-2019 02:23:03

Channel:GFP set at:12-Jul-2019 02:23:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 12-Jul-2019 02:23:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:06

Channel:Brightfield set at:12-Jul-2019 02:23:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 02:23:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:07

Channel configuration set to: GFP: 12-Jul-2019 02:23:08

Channel:GFP set at:12-Jul-2019 02:23:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 02:23:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:12

Channel:Brightfield set at:12-Jul-2019 02:23:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 02:23:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:13

Channel configuration set to: GFP: 12-Jul-2019 02:23:14

Channel:GFP set at:12-Jul-2019 02:23:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 02:23:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.725

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:17

Channel:Brightfield set at:12-Jul-2019 02:23:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 02:23:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:18

Channel configuration set to: GFP: 12-Jul-2019 02:23:19

Channel:GFP set at:12-Jul-2019 02:23:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 12-Jul-2019 02:23:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:23

Channel:Brightfield set at:12-Jul-2019 02:23:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 02:23:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:23

Channel configuration set to: GFP: 12-Jul-2019 02:23:24

Channel:GFP set at:12-Jul-2019 02:23:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 02:23:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:28

Channel:Brightfield set at:12-Jul-2019 02:23:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 02:23:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:28

Channel configuration set to: GFP: 12-Jul-2019 02:23:29

Channel:GFP set at:12-Jul-2019 02:23:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 02:23:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:33

Channel:Brightfield set at:12-Jul-2019 02:23:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2251. 12-Jul-2019 02:23:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:33

Channel configuration set to: GFP: 12-Jul-2019 02:23:34

Channel:GFP set at:12-Jul-2019 02:23:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 12-Jul-2019 02:23:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:38

Channel:Brightfield set at:12-Jul-2019 02:23:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 02:23:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:39

Channel configuration set to: GFP: 12-Jul-2019 02:23:40

Channel:GFP set at:12-Jul-2019 02:23:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 02:23:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:44

Channel:Brightfield set at:12-Jul-2019 02:23:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 02:23:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:45

Channel configuration set to: GFP: 12-Jul-2019 02:23:46

Channel:GFP set at:12-Jul-2019 02:23:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7001. 12-Jul-2019 02:23:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:49

Channel:Brightfield set at:12-Jul-2019 02:23:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 02:23:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:49

Channel configuration set to: GFP: 12-Jul-2019 02:23:51

Channel:GFP set at:12-Jul-2019 02:23:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 02:23:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:54

Channel:Brightfield set at:12-Jul-2019 02:23:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 02:23:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:55

Channel configuration set to: GFP: 12-Jul-2019 02:23:56

Channel:GFP set at:12-Jul-2019 02:23:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 02:23:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:23:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:23:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.075

Channel configuration set to: Brightfield: 12-Jul-2019 02:24:00

Channel:Brightfield set at:12-Jul-2019 02:24:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6501. 12-Jul-2019 02:24:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:24:00

Channel configuration set to: GFP: 12-Jul-2019 02:24:01

Channel:GFP set at:12-Jul-2019 02:24:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 12-Jul-2019 02:24:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:24:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:24:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 02:24:05

Channel:Brightfield set at:12-Jul-2019 02:24:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6001. 12-Jul-2019 02:24:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:24:05

Channel configuration set to: GFP: 12-Jul-2019 02:24:06

Channel:GFP set at:12-Jul-2019 02:24:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 12-Jul-2019 02:24:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:24:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:120 completed at:12-Jul-2019 02:24:09

Time since start of timelapse:35795.7222

PFS is locked

Time to next time point:204.9394

Switching pumps at 12-Jul-2019 02:27:34. Fast infusion/withdrawal step: Rate=100. Volume=50

Pump switch complete at:12-Jul-2019 02:27:34

Dominant pump is now: COM7 pumping at 0ul/min

Dominant medium is now: 0% glucose in SC



------Time point_121------

Memory available for all arrays: 61081759744

Memory used by Matlab: 2214699008

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:27:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.175

Channel configuration set to: Brightfield: 12-Jul-2019 02:27:35

Channel:Brightfield set at:12-Jul-2019 02:27:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1001. 12-Jul-2019 02:27:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:27:36

Channel configuration set to: GFP: 12-Jul-2019 02:27:37

Channel:GFP set at:12-Jul-2019 02:27:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 02:27:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:27:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:27:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9

Channel configuration set to: Brightfield: 12-Jul-2019 02:27:41

Channel:Brightfield set at:12-Jul-2019 02:27:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 12-Jul-2019 02:27:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:27:41

Channel configuration set to: GFP: 12-Jul-2019 02:27:42

Channel:GFP set at:12-Jul-2019 02:27:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 12-Jul-2019 02:27:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:27:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:27:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:27:45

Channel:Brightfield set at:12-Jul-2019 02:27:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 12-Jul-2019 02:27:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:27:45

Channel configuration set to: GFP: 12-Jul-2019 02:27:47

Channel:GFP set at:12-Jul-2019 02:27:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 12-Jul-2019 02:27:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:27:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:27:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.75

Channel configuration set to: Brightfield: 12-Jul-2019 02:27:50

Channel:Brightfield set at:12-Jul-2019 02:27:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 02:27:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:27:51

Channel configuration set to: GFP: 12-Jul-2019 02:27:52

Channel:GFP set at:12-Jul-2019 02:27:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 12-Jul-2019 02:27:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:27:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:27:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.75

Channel configuration set to: Brightfield: 12-Jul-2019 02:27:56

Channel:Brightfield set at:12-Jul-2019 02:27:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:27:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:27:56

Channel configuration set to: GFP: 12-Jul-2019 02:27:57

Channel:GFP set at:12-Jul-2019 02:27:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:27:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:27:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.2

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:01

Channel:Brightfield set at:12-Jul-2019 02:28:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 02:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:02

Channel configuration set to: GFP: 12-Jul-2019 02:28:03

Channel:GFP set at:12-Jul-2019 02:28:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 02:28:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:07

Channel:Brightfield set at:12-Jul-2019 02:28:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 02:28:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:07

Channel configuration set to: GFP: 12-Jul-2019 02:28:08

Channel:GFP set at:12-Jul-2019 02:28:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 02:28:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.675

PFS not locked. Waiting for lock...

PFS took 0.750000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:12

Channel:Brightfield set at:12-Jul-2019 02:28:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 12-Jul-2019 02:28:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:13

Channel configuration set to: GFP: 12-Jul-2019 02:28:14

Channel:GFP set at:12-Jul-2019 02:28:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 12-Jul-2019 02:28:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:18

Channel:Brightfield set at:12-Jul-2019 02:28:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 02:28:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:18

Channel configuration set to: GFP: 12-Jul-2019 02:28:19

Channel:GFP set at:12-Jul-2019 02:28:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 02:28:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.275

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:22

Channel:Brightfield set at:12-Jul-2019 02:28:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 12-Jul-2019 02:28:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:23

Channel configuration set to: GFP: 12-Jul-2019 02:28:24

Channel:GFP set at:12-Jul-2019 02:28:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 12-Jul-2019 02:28:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:28

Channel:Brightfield set at:12-Jul-2019 02:28:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 02:28:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:28

Channel configuration set to: GFP: 12-Jul-2019 02:28:29

Channel:GFP set at:12-Jul-2019 02:28:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 02:28:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:33

Channel:Brightfield set at:12-Jul-2019 02:28:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 02:28:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:33

Channel configuration set to: GFP: 12-Jul-2019 02:28:34

Channel:GFP set at:12-Jul-2019 02:28:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 02:28:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:39

Channel:Brightfield set at:12-Jul-2019 02:28:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 02:28:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:39

Channel configuration set to: GFP: 12-Jul-2019 02:28:40

Channel:GFP set at:12-Jul-2019 02:28:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 02:28:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:44

Channel:Brightfield set at:12-Jul-2019 02:28:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 02:28:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:45

Channel configuration set to: GFP: 12-Jul-2019 02:28:46

Channel:GFP set at:12-Jul-2019 02:28:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 02:28:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:49

Channel:Brightfield set at:12-Jul-2019 02:28:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 02:28:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:50

Channel configuration set to: GFP: 12-Jul-2019 02:28:51

Channel:GFP set at:12-Jul-2019 02:28:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 02:28:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.8

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:54

Channel:Brightfield set at:12-Jul-2019 02:28:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 12-Jul-2019 02:28:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:55

Channel configuration set to: GFP: 12-Jul-2019 02:28:56

Channel:GFP set at:12-Jul-2019 02:28:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 12-Jul-2019 02:28:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:28:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 12-Jul-2019 02:28:59

Channel:Brightfield set at:12-Jul-2019 02:28:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 02:29:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:29:00

Channel configuration set to: GFP: 12-Jul-2019 02:29:01

Channel:GFP set at:12-Jul-2019 02:29:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 02:29:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:29:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:29:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 12-Jul-2019 02:29:05

Channel:Brightfield set at:12-Jul-2019 02:29:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 02:29:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:29:05

Channel configuration set to: GFP: 12-Jul-2019 02:29:06

Channel:GFP set at:12-Jul-2019 02:29:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 12-Jul-2019 02:29:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:29:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:121 completed at:12-Jul-2019 02:29:09

Time since start of timelapse:36095.4279

PFS is locked

Time to next time point:205.235



------Time point_122------

Memory available for all arrays: 61082505216

Memory used by Matlab: 2215952384

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:32:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.6

Channel configuration set to: Brightfield: 12-Jul-2019 02:32:35

Channel:Brightfield set at:12-Jul-2019 02:32:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 02:32:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:32:36

Channel configuration set to: GFP: 12-Jul-2019 02:32:37

Channel:GFP set at:12-Jul-2019 02:32:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7001. 12-Jul-2019 02:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:32:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:32:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 12-Jul-2019 02:32:41

Channel:Brightfield set at:12-Jul-2019 02:32:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 02:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:32:41

Channel configuration set to: GFP: 12-Jul-2019 02:32:42

Channel:GFP set at:12-Jul-2019 02:32:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 02:32:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:32:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:32:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 12-Jul-2019 02:32:45

Channel:Brightfield set at:12-Jul-2019 02:32:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 02:32:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:32:46

Channel configuration set to: GFP: 12-Jul-2019 02:32:47

Channel:GFP set at:12-Jul-2019 02:32:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 02:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:32:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:32:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.35

PFS not locked. Waiting for lock...

PFS took 0.250000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:32:50

Channel:Brightfield set at:12-Jul-2019 02:32:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 02:32:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:32:51

Channel configuration set to: GFP: 12-Jul-2019 02:32:52

Channel:GFP set at:12-Jul-2019 02:32:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 02:32:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:32:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:32:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.775

Channel configuration set to: Brightfield: 12-Jul-2019 02:32:56

Channel:Brightfield set at:12-Jul-2019 02:32:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:32:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:32:56

Channel configuration set to: GFP: 12-Jul-2019 02:32:57

Channel:GFP set at:12-Jul-2019 02:32:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:32:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:32:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.2

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:01

Channel:Brightfield set at:12-Jul-2019 02:33:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 02:33:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:01

Channel configuration set to: GFP: 12-Jul-2019 02:33:02

Channel:GFP set at:12-Jul-2019 02:33:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 12-Jul-2019 02:33:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:07

Channel:Brightfield set at:12-Jul-2019 02:33:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 02:33:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:07

Channel configuration set to: GFP: 12-Jul-2019 02:33:08

Channel:GFP set at:12-Jul-2019 02:33:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 02:33:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.65

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:12

Channel:Brightfield set at:12-Jul-2019 02:33:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 12-Jul-2019 02:33:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:13

Channel configuration set to: GFP: 12-Jul-2019 02:33:14

Channel:GFP set at:12-Jul-2019 02:33:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 12-Jul-2019 02:33:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.8

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:17

Channel:Brightfield set at:12-Jul-2019 02:33:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 02:33:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:18

Channel configuration set to: GFP: 12-Jul-2019 02:33:19

Channel:GFP set at:12-Jul-2019 02:33:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 12-Jul-2019 02:33:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:23

Channel:Brightfield set at:12-Jul-2019 02:33:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 12-Jul-2019 02:33:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:23

Channel configuration set to: GFP: 12-Jul-2019 02:33:24

Channel:GFP set at:12-Jul-2019 02:33:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 12-Jul-2019 02:33:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:28

Channel:Brightfield set at:12-Jul-2019 02:33:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 02:33:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:28

Channel configuration set to: GFP: 12-Jul-2019 02:33:30

Channel:GFP set at:12-Jul-2019 02:33:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 02:33:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:34

Channel:Brightfield set at:12-Jul-2019 02:33:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 12-Jul-2019 02:33:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:34

Channel configuration set to: GFP: 12-Jul-2019 02:33:35

Channel:GFP set at:12-Jul-2019 02:33:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 12-Jul-2019 02:33:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.15

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:39

Channel:Brightfield set at:12-Jul-2019 02:33:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 02:33:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:39

Channel configuration set to: GFP: 12-Jul-2019 02:33:41

Channel:GFP set at:12-Jul-2019 02:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 02:33:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:44

Channel:Brightfield set at:12-Jul-2019 02:33:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 02:33:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:45

Channel configuration set to: GFP: 12-Jul-2019 02:33:46

Channel:GFP set at:12-Jul-2019 02:33:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 02:33:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.75

PFS not locked. Waiting for lock...

PFS took 0.350000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:50

Channel:Brightfield set at:12-Jul-2019 02:33:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:33:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:50

Channel configuration set to: GFP: 12-Jul-2019 02:33:51

Channel:GFP set at:12-Jul-2019 02:33:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:33:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:55

Channel:Brightfield set at:12-Jul-2019 02:33:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 02:33:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:56

Channel configuration set to: GFP: 12-Jul-2019 02:33:57

Channel:GFP set at:12-Jul-2019 02:33:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6751. 12-Jul-2019 02:33:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:33:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:33:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.175

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:34:00

Channel:Brightfield set at:12-Jul-2019 02:34:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 02:34:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:34:01

Channel configuration set to: GFP: 12-Jul-2019 02:34:02

Channel:GFP set at:12-Jul-2019 02:34:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 12-Jul-2019 02:34:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:34:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:34:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.275

Channel configuration set to: Brightfield: 12-Jul-2019 02:34:05

Channel:Brightfield set at:12-Jul-2019 02:34:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 02:34:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:34:06

Channel configuration set to: GFP: 12-Jul-2019 02:34:07

Channel:GFP set at:12-Jul-2019 02:34:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 02:34:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:34:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:122 completed at:12-Jul-2019 02:34:09

Time since start of timelapse:36396.2734

PFS is locked

Time to next time point:204.3908



------Time point_123------

Memory available for all arrays: 61081657344

Memory used by Matlab: 2215952384

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:37:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.6

PFS not locked. Waiting for lock...

PFS took 0.450000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:37:36

Channel:Brightfield set at:12-Jul-2019 02:37:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 02:37:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:37:36

Channel configuration set to: GFP: 12-Jul-2019 02:37:37

Channel:GFP set at:12-Jul-2019 02:37:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 02:37:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:37:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:37:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 12-Jul-2019 02:37:41

Channel:Brightfield set at:12-Jul-2019 02:37:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 02:37:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:37:42

Channel configuration set to: GFP: 12-Jul-2019 02:37:43

Channel:GFP set at:12-Jul-2019 02:37:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6751. 12-Jul-2019 02:37:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:37:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:37:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 12-Jul-2019 02:37:46

Channel:Brightfield set at:12-Jul-2019 02:37:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 02:37:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:37:46

Channel configuration set to: GFP: 12-Jul-2019 02:37:47

Channel:GFP set at:12-Jul-2019 02:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 02:37:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:37:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:37:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.925

Channel configuration set to: Brightfield: 12-Jul-2019 02:37:51

Channel:Brightfield set at:12-Jul-2019 02:37:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 02:37:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:37:52

Channel configuration set to: GFP: 12-Jul-2019 02:37:53

Channel:GFP set at:12-Jul-2019 02:37:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 02:37:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:37:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:37:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.9

Channel configuration set to: Brightfield: 12-Jul-2019 02:37:57

Channel:Brightfield set at:12-Jul-2019 02:37:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 02:37:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:37:57

Channel configuration set to: GFP: 12-Jul-2019 02:37:58

Channel:GFP set at:12-Jul-2019 02:37:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 02:37:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:37:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.35

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:01

Channel:Brightfield set at:12-Jul-2019 02:38:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 12-Jul-2019 02:38:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:02

Channel configuration set to: GFP: 12-Jul-2019 02:38:03

Channel:GFP set at:12-Jul-2019 02:38:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 02:38:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:07

Channel:Brightfield set at:12-Jul-2019 02:38:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6501. 12-Jul-2019 02:38:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:07

Channel configuration set to: GFP: 12-Jul-2019 02:38:09

Channel:GFP set at:12-Jul-2019 02:38:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 02:38:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:13

Channel:Brightfield set at:12-Jul-2019 02:38:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2001. 12-Jul-2019 02:38:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:13

Channel configuration set to: GFP: 12-Jul-2019 02:38:14

Channel:GFP set at:12-Jul-2019 02:38:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2001. 12-Jul-2019 02:38:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:18

Channel:Brightfield set at:12-Jul-2019 02:38:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 02:38:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:18

Channel configuration set to: GFP: 12-Jul-2019 02:38:20

Channel:GFP set at:12-Jul-2019 02:38:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 02:38:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:24

Channel:Brightfield set at:12-Jul-2019 02:38:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 12-Jul-2019 02:38:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:24

Channel configuration set to: GFP: 12-Jul-2019 02:38:25

Channel:GFP set at:12-Jul-2019 02:38:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 12-Jul-2019 02:38:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:29

Channel:Brightfield set at:12-Jul-2019 02:38:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 02:38:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:29

Channel configuration set to: GFP: 12-Jul-2019 02:38:30

Channel:GFP set at:12-Jul-2019 02:38:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 02:38:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:34

Channel:Brightfield set at:12-Jul-2019 02:38:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 02:38:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:34

Channel configuration set to: GFP: 12-Jul-2019 02:38:36

Channel:GFP set at:12-Jul-2019 02:38:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 02:38:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:39

Channel:Brightfield set at:12-Jul-2019 02:38:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 02:38:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:40

Channel configuration set to: GFP: 12-Jul-2019 02:38:41

Channel:GFP set at:12-Jul-2019 02:38:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 02:38:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.925

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:44

Channel:Brightfield set at:12-Jul-2019 02:38:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 02:38:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:45

Channel configuration set to: GFP: 12-Jul-2019 02:38:46

Channel:GFP set at:12-Jul-2019 02:38:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 02:38:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:50

Channel:Brightfield set at:12-Jul-2019 02:38:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:38:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:50

Channel configuration set to: GFP: 12-Jul-2019 02:38:51

Channel:GFP set at:12-Jul-2019 02:38:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:38:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:55

Channel:Brightfield set at:12-Jul-2019 02:38:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 02:38:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:56

Channel configuration set to: GFP: 12-Jul-2019 02:38:57

Channel:GFP set at:12-Jul-2019 02:38:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 02:38:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:38:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:38:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 12-Jul-2019 02:39:00

Channel:Brightfield set at:12-Jul-2019 02:39:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 12-Jul-2019 02:39:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:39:01

Channel configuration set to: GFP: 12-Jul-2019 02:39:02

Channel:GFP set at:12-Jul-2019 02:39:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 12-Jul-2019 02:39:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:39:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:39:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 12-Jul-2019 02:39:07

Channel:Brightfield set at:12-Jul-2019 02:39:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 02:39:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:39:07

Channel configuration set to: GFP: 12-Jul-2019 02:39:08

Channel:GFP set at:12-Jul-2019 02:39:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 02:39:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:39:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:123 completed at:12-Jul-2019 02:39:11

Time since start of timelapse:36697.4709

PFS is locked

Time to next time point:203.1949



------Time point_124------

Memory available for all arrays: 61081088000

Memory used by Matlab: 2215952384

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:42:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.55

Channel configuration set to: Brightfield: 12-Jul-2019 02:42:35

Channel:Brightfield set at:12-Jul-2019 02:42:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 02:42:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:42:36

Channel configuration set to: GFP: 12-Jul-2019 02:42:37

Channel:GFP set at:12-Jul-2019 02:42:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 02:42:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:42:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:42:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 12-Jul-2019 02:42:41

Channel:Brightfield set at:12-Jul-2019 02:42:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 02:42:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:42:41

Channel configuration set to: GFP: 12-Jul-2019 02:42:42

Channel:GFP set at:12-Jul-2019 02:42:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6751. 12-Jul-2019 02:42:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:42:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:42:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 12-Jul-2019 02:42:45

Channel:Brightfield set at:12-Jul-2019 02:42:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 02:42:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:42:46

Channel configuration set to: GFP: 12-Jul-2019 02:42:47

Channel:GFP set at:12-Jul-2019 02:42:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 02:42:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:42:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:42:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.525

Channel configuration set to: Brightfield: 12-Jul-2019 02:42:51

Channel:Brightfield set at:12-Jul-2019 02:42:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 12-Jul-2019 02:42:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:42:52

Channel configuration set to: GFP: 12-Jul-2019 02:42:53

Channel:GFP set at:12-Jul-2019 02:42:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 12-Jul-2019 02:42:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:42:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:42:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.95

Channel configuration set to: Brightfield: 12-Jul-2019 02:42:57

Channel:Brightfield set at:12-Jul-2019 02:42:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 02:42:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:42:57

Channel configuration set to: GFP: 12-Jul-2019 02:42:58

Channel:GFP set at:12-Jul-2019 02:42:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 02:42:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:42:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.45

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:02

Channel:Brightfield set at:12-Jul-2019 02:43:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 02:43:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:02

Channel configuration set to: GFP: 12-Jul-2019 02:43:04

Channel:GFP set at:12-Jul-2019 02:43:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 02:43:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:08

Channel:Brightfield set at:12-Jul-2019 02:43:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 02:43:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:08

Channel configuration set to: GFP: 12-Jul-2019 02:43:09

Channel:GFP set at:12-Jul-2019 02:43:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 02:43:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.65

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:13

Channel:Brightfield set at:12-Jul-2019 02:43:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 12-Jul-2019 02:43:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:14

Channel configuration set to: GFP: 12-Jul-2019 02:43:15

Channel:GFP set at:12-Jul-2019 02:43:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3001. 12-Jul-2019 02:43:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:19

Channel:Brightfield set at:12-Jul-2019 02:43:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 02:43:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:19

Channel configuration set to: GFP: 12-Jul-2019 02:43:20

Channel:GFP set at:12-Jul-2019 02:43:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 02:43:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:24

Channel:Brightfield set at:12-Jul-2019 02:43:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 12-Jul-2019 02:43:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:25

Channel configuration set to: GFP: 12-Jul-2019 02:43:26

Channel:GFP set at:12-Jul-2019 02:43:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 12-Jul-2019 02:43:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:27

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:29

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:30

Channel:Brightfield set at:12-Jul-2019 02:43:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 02:43:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:30

Channel configuration set to: GFP: 12-Jul-2019 02:43:31

Channel:GFP set at:12-Jul-2019 02:43:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 02:43:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:36

Channel:Brightfield set at:12-Jul-2019 02:43:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 02:43:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:37

Channel configuration set to: GFP: 12-Jul-2019 02:43:38

Channel:GFP set at:12-Jul-2019 02:43:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 02:43:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:38

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:40

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:41

Channel:Brightfield set at:12-Jul-2019 02:43:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 02:43:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:42

Channel configuration set to: GFP: 12-Jul-2019 02:43:43

Channel:GFP set at:12-Jul-2019 02:43:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 02:43:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:46

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:46

Channel:Brightfield set at:12-Jul-2019 02:43:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 02:43:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:47

Channel configuration set to: GFP: 12-Jul-2019 02:43:48

Channel:GFP set at:12-Jul-2019 02:43:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 02:43:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.625

PFS not locked. Waiting for lock...

PFS took 0.350000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:52

Channel:Brightfield set at:12-Jul-2019 02:43:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:43:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:52

Channel configuration set to: GFP: 12-Jul-2019 02:43:53

Channel:GFP set at:12-Jul-2019 02:43:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:43:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:54

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 12-Jul-2019 02:43:57

Channel:Brightfield set at:12-Jul-2019 02:43:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 02:43:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:57

Channel configuration set to: GFP: 12-Jul-2019 02:43:59

Channel:GFP set at:12-Jul-2019 02:43:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6251. 12-Jul-2019 02:43:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:43:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:44:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 12-Jul-2019 02:44:02

Channel:Brightfield set at:12-Jul-2019 02:44:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 02:44:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:44:02

Channel configuration set to: GFP: 12-Jul-2019 02:44:04

Channel:GFP set at:12-Jul-2019 02:44:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 02:44:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:44:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:44:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 12-Jul-2019 02:44:07

Channel:Brightfield set at:12-Jul-2019 02:44:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 02:44:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:44:08

Channel configuration set to: GFP: 12-Jul-2019 02:44:09

Channel:GFP set at:12-Jul-2019 02:44:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 02:44:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:44:10

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:124 completed at:12-Jul-2019 02:44:11

Time since start of timelapse:36998.2677

PFS is locked

Time to next time point:202.3994



------Time point_125------

Memory available for all arrays: 61076688896

Memory used by Matlab: 2215952384

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:47:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.8

Channel configuration set to: Brightfield: 12-Jul-2019 02:47:35

Channel:Brightfield set at:12-Jul-2019 02:47:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 02:47:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:47:36

Channel configuration set to: GFP: 12-Jul-2019 02:47:37

Channel:GFP set at:12-Jul-2019 02:47:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 02:47:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:47:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:47:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.3

Channel configuration set to: Brightfield: 12-Jul-2019 02:47:41

Channel:Brightfield set at:12-Jul-2019 02:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 02:47:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:47:41

Channel configuration set to: GFP: 12-Jul-2019 02:47:43

Channel:GFP set at:12-Jul-2019 02:47:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 02:47:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:47:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:47:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.5

Channel configuration set to: Brightfield: 12-Jul-2019 02:47:46

Channel:Brightfield set at:12-Jul-2019 02:47:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 12-Jul-2019 02:47:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:47:46

Channel configuration set to: GFP: 12-Jul-2019 02:47:48

Channel:GFP set at:12-Jul-2019 02:47:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 12-Jul-2019 02:47:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:47:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:47:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.15

Channel configuration set to: Brightfield: 12-Jul-2019 02:47:51

Channel:Brightfield set at:12-Jul-2019 02:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 02:47:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:47:52

Channel configuration set to: GFP: 12-Jul-2019 02:47:53

Channel:GFP set at:12-Jul-2019 02:47:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 02:47:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:47:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:47:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.175

Channel configuration set to: Brightfield: 12-Jul-2019 02:47:57

Channel:Brightfield set at:12-Jul-2019 02:47:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 02:47:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:47:57

Channel configuration set to: GFP: 12-Jul-2019 02:47:58

Channel:GFP set at:12-Jul-2019 02:47:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 02:47:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:47:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:02

Channel:Brightfield set at:12-Jul-2019 02:48:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 12-Jul-2019 02:48:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:02

Channel configuration set to: GFP: 12-Jul-2019 02:48:03

Channel:GFP set at:12-Jul-2019 02:48:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 12-Jul-2019 02:48:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:08

Channel:Brightfield set at:12-Jul-2019 02:48:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 02:48:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:08

Channel configuration set to: GFP: 12-Jul-2019 02:48:09

Channel:GFP set at:12-Jul-2019 02:48:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 02:48:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.85

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:13

Channel:Brightfield set at:12-Jul-2019 02:48:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 12-Jul-2019 02:48:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:14

Channel configuration set to: GFP: 12-Jul-2019 02:48:15

Channel:GFP set at:12-Jul-2019 02:48:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 12-Jul-2019 02:48:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:19

Channel:Brightfield set at:12-Jul-2019 02:48:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 02:48:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:19

Channel configuration set to: GFP: 12-Jul-2019 02:48:20

Channel:GFP set at:12-Jul-2019 02:48:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 02:48:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.625

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:24

Channel:Brightfield set at:12-Jul-2019 02:48:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 02:48:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:24

Channel configuration set to: GFP: 12-Jul-2019 02:48:26

Channel:GFP set at:12-Jul-2019 02:48:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 02:48:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:29

Channel:Brightfield set at:12-Jul-2019 02:48:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8501. 12-Jul-2019 02:48:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:29

Channel configuration set to: GFP: 12-Jul-2019 02:48:31

Channel:GFP set at:12-Jul-2019 02:48:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8501. 12-Jul-2019 02:48:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:35

Channel:Brightfield set at:12-Jul-2019 02:48:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 02:48:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:35

Channel configuration set to: GFP: 12-Jul-2019 02:48:36

Channel:GFP set at:12-Jul-2019 02:48:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 02:48:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:40

Channel:Brightfield set at:12-Jul-2019 02:48:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 02:48:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:41

Channel configuration set to: GFP: 12-Jul-2019 02:48:42

Channel:GFP set at:12-Jul-2019 02:48:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 02:48:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:45

Channel:Brightfield set at:12-Jul-2019 02:48:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 02:48:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:46

Channel configuration set to: GFP: 12-Jul-2019 02:48:47

Channel:GFP set at:12-Jul-2019 02:48:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 02:48:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:51

Channel:Brightfield set at:12-Jul-2019 02:48:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 02:48:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:51

Channel configuration set to: GFP: 12-Jul-2019 02:48:52

Channel:GFP set at:12-Jul-2019 02:48:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 02:48:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 12-Jul-2019 02:48:56

Channel:Brightfield set at:12-Jul-2019 02:48:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 02:48:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:57

Channel configuration set to: GFP: 12-Jul-2019 02:48:58

Channel:GFP set at:12-Jul-2019 02:48:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 02:48:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:48:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:49:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 02:49:01

Channel:Brightfield set at:12-Jul-2019 02:49:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 12-Jul-2019 02:49:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:49:01

Channel configuration set to: GFP: 12-Jul-2019 02:49:02

Channel:GFP set at:12-Jul-2019 02:49:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 12-Jul-2019 02:49:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:49:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:49:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 12-Jul-2019 02:49:06

Channel:Brightfield set at:12-Jul-2019 02:49:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 02:49:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:49:06

Channel configuration set to: GFP: 12-Jul-2019 02:49:08

Channel:GFP set at:12-Jul-2019 02:49:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 02:49:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:49:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:125 completed at:12-Jul-2019 02:49:10

Time since start of timelapse:37296.7115

PFS is locked

Time to next time point:203.9572



------Time point_126------

Memory available for all arrays: 61080576000

Memory used by Matlab: 2215952384

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:52:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.775

Channel configuration set to: Brightfield: 12-Jul-2019 02:52:35

Channel:Brightfield set at:12-Jul-2019 02:52:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 02:52:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:52:36

Channel configuration set to: GFP: 12-Jul-2019 02:52:37

Channel:GFP set at:12-Jul-2019 02:52:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 02:52:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:52:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:52:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.3

Channel configuration set to: Brightfield: 12-Jul-2019 02:52:40

Channel:Brightfield set at:12-Jul-2019 02:52:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 02:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:52:41

Channel configuration set to: GFP: 12-Jul-2019 02:52:42

Channel:GFP set at:12-Jul-2019 02:52:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 02:52:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:52:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:52:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.6

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:52:45

Channel:Brightfield set at:12-Jul-2019 02:52:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 12-Jul-2019 02:52:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:52:45

Channel configuration set to: GFP: 12-Jul-2019 02:52:47

Channel:GFP set at:12-Jul-2019 02:52:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 02:52:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:52:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:52:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.075

Channel configuration set to: Brightfield: 12-Jul-2019 02:52:51

Channel:Brightfield set at:12-Jul-2019 02:52:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 02:52:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:52:51

Channel configuration set to: GFP: 12-Jul-2019 02:52:52

Channel:GFP set at:12-Jul-2019 02:52:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 02:52:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:52:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:52:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:52:56

Channel:Brightfield set at:12-Jul-2019 02:52:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 02:52:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:52:57

Channel configuration set to: GFP: 12-Jul-2019 02:52:58

Channel:GFP set at:12-Jul-2019 02:52:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 02:52:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:52:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:02

Channel:Brightfield set at:12-Jul-2019 02:53:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 02:53:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:02

Channel configuration set to: GFP: 12-Jul-2019 02:53:03

Channel:GFP set at:12-Jul-2019 02:53:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 02:53:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:07

Channel:Brightfield set at:12-Jul-2019 02:53:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 02:53:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:08

Channel configuration set to: GFP: 12-Jul-2019 02:53:09

Channel:GFP set at:12-Jul-2019 02:53:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 02:53:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.875

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:13

Channel:Brightfield set at:12-Jul-2019 02:53:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 02:53:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:13

Channel configuration set to: GFP: 12-Jul-2019 02:53:15

Channel:GFP set at:12-Jul-2019 02:53:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 12-Jul-2019 02:53:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.15

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:18

Channel:Brightfield set at:12-Jul-2019 02:53:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 02:53:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:19

Channel configuration set to: GFP: 12-Jul-2019 02:53:20

Channel:GFP set at:12-Jul-2019 02:53:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 02:53:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.625

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:23

Channel:Brightfield set at:12-Jul-2019 02:53:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 02:53:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:23

Channel configuration set to: GFP: 12-Jul-2019 02:53:25

Channel:GFP set at:12-Jul-2019 02:53:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 02:53:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:28

Channel:Brightfield set at:12-Jul-2019 02:53:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8501. 12-Jul-2019 02:53:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:29

Channel configuration set to: GFP: 12-Jul-2019 02:53:30

Channel:GFP set at:12-Jul-2019 02:53:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8501. 12-Jul-2019 02:53:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:34

Channel:Brightfield set at:12-Jul-2019 02:53:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 02:53:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:34

Channel configuration set to: GFP: 12-Jul-2019 02:53:36

Channel:GFP set at:12-Jul-2019 02:53:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 02:53:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:39

Channel:Brightfield set at:12-Jul-2019 02:53:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 02:53:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:40

Channel configuration set to: GFP: 12-Jul-2019 02:53:41

Channel:GFP set at:12-Jul-2019 02:53:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 02:53:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:45

Channel:Brightfield set at:12-Jul-2019 02:53:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:53:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:46

Channel configuration set to: GFP: 12-Jul-2019 02:53:47

Channel:GFP set at:12-Jul-2019 02:53:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 02:53:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:50

Channel:Brightfield set at:12-Jul-2019 02:53:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 02:53:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:51

Channel configuration set to: GFP: 12-Jul-2019 02:53:52

Channel:GFP set at:12-Jul-2019 02:53:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 02:53:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.95

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:55

Channel:Brightfield set at:12-Jul-2019 02:53:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5501. 12-Jul-2019 02:53:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:56

Channel configuration set to: GFP: 12-Jul-2019 02:53:57

Channel:GFP set at:12-Jul-2019 02:53:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5501. 12-Jul-2019 02:53:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:53:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:53:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 12-Jul-2019 02:54:01

Channel:Brightfield set at:12-Jul-2019 02:54:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 12-Jul-2019 02:54:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:54:01

Channel configuration set to: GFP: 12-Jul-2019 02:54:02

Channel:GFP set at:12-Jul-2019 02:54:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 12-Jul-2019 02:54:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:54:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:54:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 12-Jul-2019 02:54:06

Channel:Brightfield set at:12-Jul-2019 02:54:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 02:54:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:54:06

Channel configuration set to: GFP: 12-Jul-2019 02:54:08

Channel:GFP set at:12-Jul-2019 02:54:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 02:54:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:54:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:126 completed at:12-Jul-2019 02:54:10

Time since start of timelapse:37596.6574

PFS is locked

Time to next time point:204.0128



------Time point_127------

Memory available for all arrays: 61083660288

Memory used by Matlab: 2215952384

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:57:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.625

Channel configuration set to: Brightfield: 12-Jul-2019 02:57:35

Channel:Brightfield set at:12-Jul-2019 02:57:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 02:57:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:57:36

Channel configuration set to: GFP: 12-Jul-2019 02:57:37

Channel:GFP set at:12-Jul-2019 02:57:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 02:57:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:57:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:57:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 12-Jul-2019 02:57:40

Channel:Brightfield set at:12-Jul-2019 02:57:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 02:57:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:57:41

Channel configuration set to: GFP: 12-Jul-2019 02:57:42

Channel:GFP set at:12-Jul-2019 02:57:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 02:57:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:57:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:57:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.475

Channel configuration set to: Brightfield: 12-Jul-2019 02:57:45

Channel:Brightfield set at:12-Jul-2019 02:57:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 12-Jul-2019 02:57:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:57:46

Channel configuration set to: GFP: 12-Jul-2019 02:57:47

Channel:GFP set at:12-Jul-2019 02:57:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 12-Jul-2019 02:57:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:57:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:57:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.025

Channel configuration set to: Brightfield: 12-Jul-2019 02:57:51

Channel:Brightfield set at:12-Jul-2019 02:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 02:57:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:57:51

Channel configuration set to: GFP: 12-Jul-2019 02:57:53

Channel:GFP set at:12-Jul-2019 02:57:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 02:57:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:57:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:57:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 12-Jul-2019 02:57:56

Channel:Brightfield set at:12-Jul-2019 02:57:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 02:57:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:57:56

Channel configuration set to: GFP: 12-Jul-2019 02:57:58

Channel:GFP set at:12-Jul-2019 02:57:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 02:57:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:57:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.425

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:02

Channel:Brightfield set at:12-Jul-2019 02:58:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 02:58:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:02

Channel configuration set to: GFP: 12-Jul-2019 02:58:03

Channel:GFP set at:12-Jul-2019 02:58:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 02:58:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:08

Channel:Brightfield set at:12-Jul-2019 02:58:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 02:58:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:08

Channel configuration set to: GFP: 12-Jul-2019 02:58:09

Channel:GFP set at:12-Jul-2019 02:58:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 02:58:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.925

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:13

Channel:Brightfield set at:12-Jul-2019 02:58:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0001. 12-Jul-2019 02:58:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:14

Channel configuration set to: GFP: 12-Jul-2019 02:58:15

Channel:GFP set at:12-Jul-2019 02:58:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0001. 12-Jul-2019 02:58:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:19

Channel:Brightfield set at:12-Jul-2019 02:58:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 02:58:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:19

Channel configuration set to: GFP: 12-Jul-2019 02:58:20

Channel:GFP set at:12-Jul-2019 02:58:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 02:58:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:24

Channel:Brightfield set at:12-Jul-2019 02:58:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 02:58:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:24

Channel configuration set to: GFP: 12-Jul-2019 02:58:25

Channel:GFP set at:12-Jul-2019 02:58:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 02:58:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:29

Channel:Brightfield set at:12-Jul-2019 02:58:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 02:58:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:29

Channel configuration set to: GFP: 12-Jul-2019 02:58:31

Channel:GFP set at:12-Jul-2019 02:58:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7501. 12-Jul-2019 02:58:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:35

Channel:Brightfield set at:12-Jul-2019 02:58:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 02:58:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:35

Channel configuration set to: GFP: 12-Jul-2019 02:58:36

Channel:GFP set at:12-Jul-2019 02:58:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 02:58:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:40

Channel:Brightfield set at:12-Jul-2019 02:58:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 02:58:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:40

Channel configuration set to: GFP: 12-Jul-2019 02:58:42

Channel:GFP set at:12-Jul-2019 02:58:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 12-Jul-2019 02:58:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:46

Channel:Brightfield set at:12-Jul-2019 02:58:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 02:58:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:46

Channel configuration set to: GFP: 12-Jul-2019 02:58:47

Channel:GFP set at:12-Jul-2019 02:58:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 02:58:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:51

Channel:Brightfield set at:12-Jul-2019 02:58:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 02:58:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:51

Channel configuration set to: GFP: 12-Jul-2019 02:58:52

Channel:GFP set at:12-Jul-2019 02:58:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 02:58:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 12-Jul-2019 02:58:57

Channel:Brightfield set at:12-Jul-2019 02:58:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 02:58:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:57

Channel configuration set to: GFP: 12-Jul-2019 02:58:58

Channel:GFP set at:12-Jul-2019 02:58:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 02:58:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:58:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:59:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.55

Channel configuration set to: Brightfield: 12-Jul-2019 02:59:02

Channel:Brightfield set at:12-Jul-2019 02:59:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 12-Jul-2019 02:59:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:59:02

Channel configuration set to: GFP: 12-Jul-2019 02:59:03

Channel:GFP set at:12-Jul-2019 02:59:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 12-Jul-2019 02:59:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:59:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 02:59:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 12-Jul-2019 02:59:07

Channel:Brightfield set at:12-Jul-2019 02:59:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9251. 12-Jul-2019 02:59:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:59:07

Channel configuration set to: GFP: 12-Jul-2019 02:59:09

Channel:GFP set at:12-Jul-2019 02:59:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 02:59:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 02:59:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:127 completed at:12-Jul-2019 02:59:11

Time since start of timelapse:37897.8031

PFS is locked

Time to next time point:202.8687



------Time point_128------

Memory available for all arrays: 61086314496

Memory used by Matlab: 2216214528

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:02:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.8

Channel configuration set to: Brightfield: 12-Jul-2019 03:02:35

Channel:Brightfield set at:12-Jul-2019 03:02:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 03:02:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:02:36

Channel configuration set to: GFP: 12-Jul-2019 03:02:37

Channel:GFP set at:12-Jul-2019 03:02:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 03:02:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:02:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:02:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.375

Channel configuration set to: Brightfield: 12-Jul-2019 03:02:40

Channel:Brightfield set at:12-Jul-2019 03:02:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 03:02:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:02:40

Channel configuration set to: GFP: 12-Jul-2019 03:02:42

Channel:GFP set at:12-Jul-2019 03:02:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 03:02:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:02:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:02:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.575

Channel configuration set to: Brightfield: 12-Jul-2019 03:02:45

Channel:Brightfield set at:12-Jul-2019 03:02:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 12-Jul-2019 03:02:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:02:45

Channel configuration set to: GFP: 12-Jul-2019 03:02:46

Channel:GFP set at:12-Jul-2019 03:02:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 12-Jul-2019 03:02:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:02:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:02:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.175

Channel configuration set to: Brightfield: 12-Jul-2019 03:02:50

Channel:Brightfield set at:12-Jul-2019 03:02:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 03:02:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:02:50

Channel configuration set to: GFP: 12-Jul-2019 03:02:52

Channel:GFP set at:12-Jul-2019 03:02:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 03:02:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:02:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:02:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.175

Channel configuration set to: Brightfield: 12-Jul-2019 03:02:55

Channel:Brightfield set at:12-Jul-2019 03:02:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 03:02:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:02:56

Channel configuration set to: GFP: 12-Jul-2019 03:02:57

Channel:GFP set at:12-Jul-2019 03:02:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 03:02:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:02:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.65

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:00

Channel:Brightfield set at:12-Jul-2019 03:03:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 03:03:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:01

Channel configuration set to: GFP: 12-Jul-2019 03:03:02

Channel:GFP set at:12-Jul-2019 03:03:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 03:03:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:02

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:06

Channel:Brightfield set at:12-Jul-2019 03:03:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 03:03:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:06

Channel configuration set to: GFP: 12-Jul-2019 03:03:07

Channel:GFP set at:12-Jul-2019 03:03:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 03:03:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:11

Channel:Brightfield set at:12-Jul-2019 03:03:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 03:03:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:11

Channel configuration set to: GFP: 12-Jul-2019 03:03:12

Channel:GFP set at:12-Jul-2019 03:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 03:03:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:16

Channel:Brightfield set at:12-Jul-2019 03:03:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 03:03:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:17

Channel configuration set to: GFP: 12-Jul-2019 03:03:18

Channel:GFP set at:12-Jul-2019 03:03:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 03:03:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:22

Channel:Brightfield set at:12-Jul-2019 03:03:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 03:03:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:22

Channel configuration set to: GFP: 12-Jul-2019 03:03:23

Channel:GFP set at:12-Jul-2019 03:03:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 03:03:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:26

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.475

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:27

Channel:Brightfield set at:12-Jul-2019 03:03:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7251. 12-Jul-2019 03:03:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:27

Channel configuration set to: GFP: 12-Jul-2019 03:03:28

Channel:GFP set at:12-Jul-2019 03:03:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7501. 12-Jul-2019 03:03:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:29

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:32

Channel:Brightfield set at:12-Jul-2019 03:03:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 03:03:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:32

Channel configuration set to: GFP: 12-Jul-2019 03:03:33

Channel:GFP set at:12-Jul-2019 03:03:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 03:03:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:37

Channel:Brightfield set at:12-Jul-2019 03:03:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 03:03:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:38

Channel configuration set to: GFP: 12-Jul-2019 03:03:39

Channel:GFP set at:12-Jul-2019 03:03:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 03:03:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:42

Channel:Brightfield set at:12-Jul-2019 03:03:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 03:03:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:42

Channel configuration set to: GFP: 12-Jul-2019 03:03:43

Channel:GFP set at:12-Jul-2019 03:03:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6501. 12-Jul-2019 03:03:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:46

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.925

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:47

Channel:Brightfield set at:12-Jul-2019 03:03:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 03:03:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:47

Channel configuration set to: GFP: 12-Jul-2019 03:03:48

Channel:GFP set at:12-Jul-2019 03:03:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 03:03:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:49

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:51

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:52

Channel:Brightfield set at:12-Jul-2019 03:03:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 03:03:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:52

Channel configuration set to: GFP: 12-Jul-2019 03:03:54

Channel:GFP set at:12-Jul-2019 03:03:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4251. 12-Jul-2019 03:03:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:54

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:56

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 12-Jul-2019 03:03:58

Channel:Brightfield set at:12-Jul-2019 03:03:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 03:03:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:03:58

Channel configuration set to: GFP: 12-Jul-2019 03:03:59

Channel:GFP set at:12-Jul-2019 03:03:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 03:04:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:04:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:04:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 12-Jul-2019 03:04:03

Channel:Brightfield set at:12-Jul-2019 03:04:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 03:04:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:04:03

Channel configuration set to: GFP: 12-Jul-2019 03:04:04

Channel:GFP set at:12-Jul-2019 03:04:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 03:04:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:04:05

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:128 completed at:12-Jul-2019 03:04:07

Time since start of timelapse:38193.7453

PFS is locked

Time to next time point:206.9277



------Time point_129------

Memory available for all arrays: 61068791808

Memory used by Matlab: 2216476672

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:07:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.2

Channel configuration set to: Brightfield: 12-Jul-2019 03:07:36

Channel:Brightfield set at:12-Jul-2019 03:07:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 03:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:07:36

Channel configuration set to: GFP: 12-Jul-2019 03:07:37

Channel:GFP set at:12-Jul-2019 03:07:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 03:07:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:07:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:07:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 03:07:41

Channel:Brightfield set at:12-Jul-2019 03:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 03:07:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:07:42

Channel configuration set to: GFP: 12-Jul-2019 03:07:43

Channel:GFP set at:12-Jul-2019 03:07:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 03:07:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:07:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:07:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 12-Jul-2019 03:07:46

Channel:Brightfield set at:12-Jul-2019 03:07:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 03:07:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:07:47

Channel configuration set to: GFP: 12-Jul-2019 03:07:48

Channel:GFP set at:12-Jul-2019 03:07:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 12-Jul-2019 03:07:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:07:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:07:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.125

Channel configuration set to: Brightfield: 12-Jul-2019 03:07:52

Channel:Brightfield set at:12-Jul-2019 03:07:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 03:07:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:07:52

Channel configuration set to: GFP: 12-Jul-2019 03:07:53

Channel:GFP set at:12-Jul-2019 03:07:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 03:07:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:07:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:07:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.125

Channel configuration set to: Brightfield: 12-Jul-2019 03:07:57

Channel:Brightfield set at:12-Jul-2019 03:07:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 03:07:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:07:58

Channel configuration set to: GFP: 12-Jul-2019 03:07:59

Channel:GFP set at:12-Jul-2019 03:07:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 03:07:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:07:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:02

Channel:Brightfield set at:12-Jul-2019 03:08:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 03:08:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:03

Channel configuration set to: GFP: 12-Jul-2019 03:08:04

Channel:GFP set at:12-Jul-2019 03:08:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 03:08:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:08

Channel:Brightfield set at:12-Jul-2019 03:08:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 03:08:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:09

Channel configuration set to: GFP: 12-Jul-2019 03:08:10

Channel:GFP set at:12-Jul-2019 03:08:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 03:08:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:13

Channel:Brightfield set at:12-Jul-2019 03:08:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 03:08:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:13

Channel configuration set to: GFP: 12-Jul-2019 03:08:15

Channel:GFP set at:12-Jul-2019 03:08:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 03:08:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:19

Channel:Brightfield set at:12-Jul-2019 03:08:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 03:08:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:19

Channel configuration set to: GFP: 12-Jul-2019 03:08:20

Channel:GFP set at:12-Jul-2019 03:08:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 03:08:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:23

Channel:Brightfield set at:12-Jul-2019 03:08:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 03:08:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:24

Channel configuration set to: GFP: 12-Jul-2019 03:08:25

Channel:GFP set at:12-Jul-2019 03:08:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 03:08:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:29

Channel:Brightfield set at:12-Jul-2019 03:08:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 03:08:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:30

Channel configuration set to: GFP: 12-Jul-2019 03:08:31

Channel:GFP set at:12-Jul-2019 03:08:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 03:08:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:34

Channel:Brightfield set at:12-Jul-2019 03:08:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9001. 12-Jul-2019 03:08:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:35

Channel configuration set to: GFP: 12-Jul-2019 03:08:36

Channel:GFP set at:12-Jul-2019 03:08:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9001. 12-Jul-2019 03:08:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:40

Channel:Brightfield set at:12-Jul-2019 03:08:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 12-Jul-2019 03:08:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:41

Channel configuration set to: GFP: 12-Jul-2019 03:08:42

Channel:GFP set at:12-Jul-2019 03:08:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 03:08:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:45

Channel:Brightfield set at:12-Jul-2019 03:08:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 03:08:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:46

Channel configuration set to: GFP: 12-Jul-2019 03:08:47

Channel:GFP set at:12-Jul-2019 03:08:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 03:08:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:51

Channel:Brightfield set at:12-Jul-2019 03:08:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 03:08:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:51

Channel configuration set to: GFP: 12-Jul-2019 03:08:52

Channel:GFP set at:12-Jul-2019 03:08:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 03:08:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 12-Jul-2019 03:08:56

Channel:Brightfield set at:12-Jul-2019 03:08:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4251. 12-Jul-2019 03:08:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:56

Channel configuration set to: GFP: 12-Jul-2019 03:08:58

Channel:GFP set at:12-Jul-2019 03:08:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 03:08:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:08:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:09:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 12-Jul-2019 03:09:01

Channel:Brightfield set at:12-Jul-2019 03:09:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 12-Jul-2019 03:09:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:09:02

Channel configuration set to: GFP: 12-Jul-2019 03:09:03

Channel:GFP set at:12-Jul-2019 03:09:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 12-Jul-2019 03:09:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:09:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:09:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5.2

Channel configuration set to: Brightfield: 12-Jul-2019 03:09:06

Channel:Brightfield set at:12-Jul-2019 03:09:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 03:09:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:09:07

Channel configuration set to: GFP: 12-Jul-2019 03:09:08

Channel:GFP set at:12-Jul-2019 03:09:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 03:09:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:09:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:129 completed at:12-Jul-2019 03:09:10

Time since start of timelapse:38497.3445

PFS is locked

Time to next time point:203.3298



------Time point_130------

Memory available for all arrays: 61038010368

Memory used by Matlab: 2216476672

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:12:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-12.35

Channel configuration set to: Brightfield: 12-Jul-2019 03:12:35

Channel:Brightfield set at:12-Jul-2019 03:12:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 03:12:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:12:35

Channel configuration set to: GFP: 12-Jul-2019 03:12:36

Channel:GFP set at:12-Jul-2019 03:12:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 03:12:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:12:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:12:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-11.1

Channel configuration set to: Brightfield: 12-Jul-2019 03:12:40

Channel:Brightfield set at:12-Jul-2019 03:12:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 12-Jul-2019 03:12:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:12:41

Channel configuration set to: GFP: 12-Jul-2019 03:12:42

Channel:GFP set at:12-Jul-2019 03:12:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 12-Jul-2019 03:12:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:12:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:12:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.175

Channel configuration set to: Brightfield: 12-Jul-2019 03:12:46

Channel:Brightfield set at:12-Jul-2019 03:12:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 12-Jul-2019 03:12:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:12:46

Channel configuration set to: GFP: 12-Jul-2019 03:12:47

Channel:GFP set at:12-Jul-2019 03:12:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7751. 12-Jul-2019 03:12:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:12:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:12:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.125

Channel configuration set to: Brightfield: 12-Jul-2019 03:12:51

Channel:Brightfield set at:12-Jul-2019 03:12:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 03:12:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:12:52

Channel configuration set to: GFP: 12-Jul-2019 03:12:53

Channel:GFP set at:12-Jul-2019 03:12:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 03:12:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:12:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:12:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 12-Jul-2019 03:12:57

Channel:Brightfield set at:12-Jul-2019 03:12:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 03:12:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:12:57

Channel configuration set to: GFP: 12-Jul-2019 03:12:59

Channel:GFP set at:12-Jul-2019 03:12:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 03:12:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:12:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.425

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:03

Channel:Brightfield set at:12-Jul-2019 03:13:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 03:13:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:03

Channel configuration set to: GFP: 12-Jul-2019 03:13:04

Channel:GFP set at:12-Jul-2019 03:13:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 03:13:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:08

Channel:Brightfield set at:12-Jul-2019 03:13:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 03:13:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:09

Channel configuration set to: GFP: 12-Jul-2019 03:13:10

Channel:GFP set at:12-Jul-2019 03:13:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 03:13:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.875

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:14

Channel:Brightfield set at:12-Jul-2019 03:13:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 12-Jul-2019 03:13:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:14

Channel configuration set to: GFP: 12-Jul-2019 03:13:16

Channel:GFP set at:12-Jul-2019 03:13:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 12-Jul-2019 03:13:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:19

Channel:Brightfield set at:12-Jul-2019 03:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 12-Jul-2019 03:13:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:19

Channel configuration set to: GFP: 12-Jul-2019 03:13:20

Channel:GFP set at:12-Jul-2019 03:13:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 12-Jul-2019 03:13:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:24

Channel:Brightfield set at:12-Jul-2019 03:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 03:13:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:25

Channel configuration set to: GFP: 12-Jul-2019 03:13:26

Channel:GFP set at:12-Jul-2019 03:13:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 03:13:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:29

Channel:Brightfield set at:12-Jul-2019 03:13:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 03:13:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:30

Channel configuration set to: GFP: 12-Jul-2019 03:13:31

Channel:GFP set at:12-Jul-2019 03:13:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 03:13:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:35

Channel:Brightfield set at:12-Jul-2019 03:13:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 03:13:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:36

Channel configuration set to: GFP: 12-Jul-2019 03:13:37

Channel:GFP set at:12-Jul-2019 03:13:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 03:13:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:40

Channel:Brightfield set at:12-Jul-2019 03:13:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 03:13:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:41

Channel configuration set to: GFP: 12-Jul-2019 03:13:42

Channel:GFP set at:12-Jul-2019 03:13:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 03:13:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:46

Channel:Brightfield set at:12-Jul-2019 03:13:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 03:13:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:46

Channel configuration set to: GFP: 12-Jul-2019 03:13:48

Channel:GFP set at:12-Jul-2019 03:13:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 03:13:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:51

Channel:Brightfield set at:12-Jul-2019 03:13:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 03:13:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:52

Channel configuration set to: GFP: 12-Jul-2019 03:13:53

Channel:GFP set at:12-Jul-2019 03:13:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 03:13:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 12-Jul-2019 03:13:57

Channel:Brightfield set at:12-Jul-2019 03:13:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 03:13:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:57

Channel configuration set to: GFP: 12-Jul-2019 03:13:59

Channel:GFP set at:12-Jul-2019 03:13:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 03:13:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:13:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:14:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.325

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:14:02

Channel:Brightfield set at:12-Jul-2019 03:14:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 12-Jul-2019 03:14:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:14:02

Channel configuration set to: GFP: 12-Jul-2019 03:14:03

Channel:GFP set at:12-Jul-2019 03:14:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 12-Jul-2019 03:14:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:14:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:14:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 03:14:08

Channel:Brightfield set at:12-Jul-2019 03:14:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 12-Jul-2019 03:14:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:14:08

Channel configuration set to: GFP: 12-Jul-2019 03:14:09

Channel:GFP set at:12-Jul-2019 03:14:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 12-Jul-2019 03:14:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:14:10

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:130 completed at:12-Jul-2019 03:14:12

Time since start of timelapse:38798.5928

PFS is locked

Time to next time point:202.0827



------Time point_131------

Memory available for all arrays: 61064855552

Memory used by Matlab: 2216476672

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:17:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.975

Channel configuration set to: Brightfield: 12-Jul-2019 03:17:36

Channel:Brightfield set at:12-Jul-2019 03:17:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 03:17:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:17:36

Channel configuration set to: GFP: 12-Jul-2019 03:17:37

Channel:GFP set at:12-Jul-2019 03:17:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 03:17:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:17:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:17:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.125

Channel configuration set to: Brightfield: 12-Jul-2019 03:17:42

Channel:Brightfield set at:12-Jul-2019 03:17:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 03:17:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:17:42

Channel configuration set to: GFP: 12-Jul-2019 03:17:43

Channel:GFP set at:12-Jul-2019 03:17:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 03:17:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:17:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:17:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.4

Channel configuration set to: Brightfield: 12-Jul-2019 03:17:46

Channel:Brightfield set at:12-Jul-2019 03:17:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 12-Jul-2019 03:17:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:17:47

Channel configuration set to: GFP: 12-Jul-2019 03:17:48

Channel:GFP set at:12-Jul-2019 03:17:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 12-Jul-2019 03:17:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:17:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:17:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.025

Channel configuration set to: Brightfield: 12-Jul-2019 03:17:52

Channel:Brightfield set at:12-Jul-2019 03:17:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 03:17:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:17:52

Channel configuration set to: GFP: 12-Jul-2019 03:17:53

Channel:GFP set at:12-Jul-2019 03:17:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 03:17:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:17:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:17:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.075

Channel configuration set to: Brightfield: 12-Jul-2019 03:17:57

Channel:Brightfield set at:12-Jul-2019 03:17:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 03:17:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:17:57

Channel configuration set to: GFP: 12-Jul-2019 03:17:59

Channel:GFP set at:12-Jul-2019 03:17:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 03:17:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:17:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:02

Channel:Brightfield set at:12-Jul-2019 03:18:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 03:18:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:03

Channel configuration set to: GFP: 12-Jul-2019 03:18:04

Channel:GFP set at:12-Jul-2019 03:18:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 03:18:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:08

Channel:Brightfield set at:12-Jul-2019 03:18:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 03:18:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:09

Channel configuration set to: GFP: 12-Jul-2019 03:18:10

Channel:GFP set at:12-Jul-2019 03:18:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 03:18:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:14

Channel:Brightfield set at:12-Jul-2019 03:18:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1251. 12-Jul-2019 03:18:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:14

Channel configuration set to: GFP: 12-Jul-2019 03:18:15

Channel:GFP set at:12-Jul-2019 03:18:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1251. 12-Jul-2019 03:18:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.05

PFS not locked. Waiting for lock...

PFS took 0.050000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:19

Channel:Brightfield set at:12-Jul-2019 03:18:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 12-Jul-2019 03:18:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:19

Channel configuration set to: GFP: 12-Jul-2019 03:18:20

Channel:GFP set at:12-Jul-2019 03:18:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 12-Jul-2019 03:18:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:24

Channel:Brightfield set at:12-Jul-2019 03:18:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 03:18:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:25

Channel configuration set to: GFP: 12-Jul-2019 03:18:26

Channel:GFP set at:12-Jul-2019 03:18:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 03:18:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:29

Channel:Brightfield set at:12-Jul-2019 03:18:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 03:18:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:30

Channel configuration set to: GFP: 12-Jul-2019 03:18:31

Channel:GFP set at:12-Jul-2019 03:18:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 03:18:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:35

Channel:Brightfield set at:12-Jul-2019 03:18:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 03:18:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:36

Channel configuration set to: GFP: 12-Jul-2019 03:18:37

Channel:GFP set at:12-Jul-2019 03:18:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 03:18:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:41

Channel:Brightfield set at:12-Jul-2019 03:18:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 03:18:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:41

Channel configuration set to: GFP: 12-Jul-2019 03:18:43

Channel:GFP set at:12-Jul-2019 03:18:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 03:18:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:47

Channel:Brightfield set at:12-Jul-2019 03:18:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 03:18:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:47

Channel configuration set to: GFP: 12-Jul-2019 03:18:48

Channel:GFP set at:12-Jul-2019 03:18:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 03:18:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:49

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:51

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:52

Channel:Brightfield set at:12-Jul-2019 03:18:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 03:18:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:52

Channel configuration set to: GFP: 12-Jul-2019 03:18:53

Channel:GFP set at:12-Jul-2019 03:18:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 03:18:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:54

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 12-Jul-2019 03:18:57

Channel:Brightfield set at:12-Jul-2019 03:18:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 03:18:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:58

Channel configuration set to: GFP: 12-Jul-2019 03:18:59

Channel:GFP set at:12-Jul-2019 03:18:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 03:18:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:18:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:19:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.35

Channel configuration set to: Brightfield: 12-Jul-2019 03:19:02

Channel:Brightfield set at:12-Jul-2019 03:19:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 12-Jul-2019 03:19:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:19:03

Channel configuration set to: GFP: 12-Jul-2019 03:19:04

Channel:GFP set at:12-Jul-2019 03:19:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 12-Jul-2019 03:19:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:19:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:19:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-4.825

Channel configuration set to: Brightfield: 12-Jul-2019 03:19:07

Channel:Brightfield set at:12-Jul-2019 03:19:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 03:19:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:19:07

Channel configuration set to: GFP: 12-Jul-2019 03:19:09

Channel:GFP set at:12-Jul-2019 03:19:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 03:19:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:19:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:131 completed at:12-Jul-2019 03:19:11

Time since start of timelapse:39098.2438

PFS is locked

Time to next time point:202.4331



------Time point_132------

Memory available for all arrays: 61061484544

Memory used by Matlab: 2216538112

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:22:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.025

Channel configuration set to: Brightfield: 12-Jul-2019 03:22:36

Channel:Brightfield set at:12-Jul-2019 03:22:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 03:22:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:22:36

Channel configuration set to: GFP: 12-Jul-2019 03:22:37

Channel:GFP set at:12-Jul-2019 03:22:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 03:22:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:22:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:22:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-10.95

Channel configuration set to: Brightfield: 12-Jul-2019 03:22:41

Channel:Brightfield set at:12-Jul-2019 03:22:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 03:22:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:22:41

Channel configuration set to: GFP: 12-Jul-2019 03:22:42

Channel:GFP set at:12-Jul-2019 03:22:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 03:22:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:22:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:22:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.25

Channel configuration set to: Brightfield: 12-Jul-2019 03:22:46

Channel:Brightfield set at:12-Jul-2019 03:22:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 12-Jul-2019 03:22:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:22:46

Channel configuration set to: GFP: 12-Jul-2019 03:22:48

Channel:GFP set at:12-Jul-2019 03:22:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 12-Jul-2019 03:22:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:22:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:22:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.975

Channel configuration set to: Brightfield: 12-Jul-2019 03:22:51

Channel:Brightfield set at:12-Jul-2019 03:22:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 03:22:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:22:52

Channel configuration set to: GFP: 12-Jul-2019 03:22:53

Channel:GFP set at:12-Jul-2019 03:22:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 03:22:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:22:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:22:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.025

Channel configuration set to: Brightfield: 12-Jul-2019 03:22:57

Channel:Brightfield set at:12-Jul-2019 03:22:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 03:22:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:22:57

Channel configuration set to: GFP: 12-Jul-2019 03:22:58

Channel:GFP set at:12-Jul-2019 03:22:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 03:22:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:22:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:01

Channel:Brightfield set at:12-Jul-2019 03:23:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 03:23:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:02

Channel configuration set to: GFP: 12-Jul-2019 03:23:03

Channel:GFP set at:12-Jul-2019 03:23:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 03:23:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:07

Channel:Brightfield set at:12-Jul-2019 03:23:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 03:23:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:07

Channel configuration set to: GFP: 12-Jul-2019 03:23:08

Channel:GFP set at:12-Jul-2019 03:23:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 03:23:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:12

Channel:Brightfield set at:12-Jul-2019 03:23:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 03:23:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:12

Channel configuration set to: GFP: 12-Jul-2019 03:23:14

Channel:GFP set at:12-Jul-2019 03:23:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 03:23:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.2

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:17

Channel:Brightfield set at:12-Jul-2019 03:23:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 03:23:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:18

Channel configuration set to: GFP: 12-Jul-2019 03:23:19

Channel:GFP set at:12-Jul-2019 03:23:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 03:23:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:23

Channel:Brightfield set at:12-Jul-2019 03:23:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 03:23:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:23

Channel configuration set to: GFP: 12-Jul-2019 03:23:24

Channel:GFP set at:12-Jul-2019 03:23:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 03:23:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:28

Channel:Brightfield set at:12-Jul-2019 03:23:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 03:23:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:28

Channel configuration set to: GFP: 12-Jul-2019 03:23:30

Channel:GFP set at:12-Jul-2019 03:23:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 03:23:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:34

Channel:Brightfield set at:12-Jul-2019 03:23:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 03:23:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:34

Channel configuration set to: GFP: 12-Jul-2019 03:23:35

Channel:GFP set at:12-Jul-2019 03:23:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 03:23:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:39

Channel:Brightfield set at:12-Jul-2019 03:23:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 03:23:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:39

Channel configuration set to: GFP: 12-Jul-2019 03:23:41

Channel:GFP set at:12-Jul-2019 03:23:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 03:23:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:45

Channel:Brightfield set at:12-Jul-2019 03:23:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 03:23:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:45

Channel configuration set to: GFP: 12-Jul-2019 03:23:46

Channel:GFP set at:12-Jul-2019 03:23:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 03:23:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.75

PFS not locked. Waiting for lock...

PFS took 0.350000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:50

Channel:Brightfield set at:12-Jul-2019 03:23:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 03:23:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:50

Channel configuration set to: GFP: 12-Jul-2019 03:23:52

Channel:GFP set at:12-Jul-2019 03:23:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 03:23:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 12-Jul-2019 03:23:56

Channel:Brightfield set at:12-Jul-2019 03:23:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 03:23:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:56

Channel configuration set to: GFP: 12-Jul-2019 03:23:57

Channel:GFP set at:12-Jul-2019 03:23:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 03:23:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:23:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:24:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.525

Channel configuration set to: Brightfield: 12-Jul-2019 03:24:01

Channel:Brightfield set at:12-Jul-2019 03:24:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 12-Jul-2019 03:24:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:24:01

Channel configuration set to: GFP: 12-Jul-2019 03:24:03

Channel:GFP set at:12-Jul-2019 03:24:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 12-Jul-2019 03:24:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:24:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:24:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 12-Jul-2019 03:24:06

Channel:Brightfield set at:12-Jul-2019 03:24:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 03:24:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:24:07

Channel configuration set to: GFP: 12-Jul-2019 03:24:08

Channel:GFP set at:12-Jul-2019 03:24:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 03:24:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:24:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:132 completed at:12-Jul-2019 03:24:10

Time since start of timelapse:39397.1837

PFS is locked

Time to next time point:203.4946



------Time point_133------

Memory available for all arrays: 61062225920

Memory used by Matlab: 2216574976

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:27:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-12.25

Channel configuration set to: Brightfield: 12-Jul-2019 03:27:35

Channel:Brightfield set at:12-Jul-2019 03:27:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 03:27:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:27:35

Channel configuration set to: GFP: 12-Jul-2019 03:27:36

Channel:GFP set at:12-Jul-2019 03:27:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 03:27:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:27:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:27:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 03:27:41

Channel:Brightfield set at:12-Jul-2019 03:27:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 03:27:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:27:41

Channel configuration set to: GFP: 12-Jul-2019 03:27:42

Channel:GFP set at:12-Jul-2019 03:27:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 03:27:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:27:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:27:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 12-Jul-2019 03:27:46

Channel:Brightfield set at:12-Jul-2019 03:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 12-Jul-2019 03:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:27:46

Channel configuration set to: GFP: 12-Jul-2019 03:27:47

Channel:GFP set at:12-Jul-2019 03:27:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 12-Jul-2019 03:27:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:27:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:27:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.1

Channel configuration set to: Brightfield: 12-Jul-2019 03:27:51

Channel:Brightfield set at:12-Jul-2019 03:27:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 03:27:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:27:52

Channel configuration set to: GFP: 12-Jul-2019 03:27:53

Channel:GFP set at:12-Jul-2019 03:27:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 03:27:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:27:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:27:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.1

Channel configuration set to: Brightfield: 12-Jul-2019 03:27:57

Channel:Brightfield set at:12-Jul-2019 03:27:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 03:27:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:27:57

Channel configuration set to: GFP: 12-Jul-2019 03:27:58

Channel:GFP set at:12-Jul-2019 03:27:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 03:27:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:27:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.55

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:03

Channel:Brightfield set at:12-Jul-2019 03:28:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 03:28:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:03

Channel configuration set to: GFP: 12-Jul-2019 03:28:04

Channel:GFP set at:12-Jul-2019 03:28:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 03:28:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:08

Channel:Brightfield set at:12-Jul-2019 03:28:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 03:28:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:08

Channel configuration set to: GFP: 12-Jul-2019 03:28:10

Channel:GFP set at:12-Jul-2019 03:28:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 03:28:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:14

Channel:Brightfield set at:12-Jul-2019 03:28:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 03:28:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:14

Channel configuration set to: GFP: 12-Jul-2019 03:28:15

Channel:GFP set at:12-Jul-2019 03:28:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 12-Jul-2019 03:28:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.275

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:19

Channel:Brightfield set at:12-Jul-2019 03:28:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 03:28:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:19

Channel configuration set to: GFP: 12-Jul-2019 03:28:21

Channel:GFP set at:12-Jul-2019 03:28:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 03:28:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:24

Channel:Brightfield set at:12-Jul-2019 03:28:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 03:28:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:24

Channel configuration set to: GFP: 12-Jul-2019 03:28:25

Channel:GFP set at:12-Jul-2019 03:28:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 03:28:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:30

Channel:Brightfield set at:12-Jul-2019 03:28:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 03:28:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:30

Channel configuration set to: GFP: 12-Jul-2019 03:28:31

Channel:GFP set at:12-Jul-2019 03:28:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 03:28:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:35

Channel:Brightfield set at:12-Jul-2019 03:28:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 03:28:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:36

Channel configuration set to: GFP: 12-Jul-2019 03:28:37

Channel:GFP set at:12-Jul-2019 03:28:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 03:28:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.1

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:41

Channel:Brightfield set at:12-Jul-2019 03:28:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 03:28:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:41

Channel configuration set to: GFP: 12-Jul-2019 03:28:43

Channel:GFP set at:12-Jul-2019 03:28:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 03:28:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:46

Channel:Brightfield set at:12-Jul-2019 03:28:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 03:28:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:46

Channel configuration set to: GFP: 12-Jul-2019 03:28:48

Channel:GFP set at:12-Jul-2019 03:28:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 03:28:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:51

Channel:Brightfield set at:12-Jul-2019 03:28:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 03:28:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:52

Channel configuration set to: GFP: 12-Jul-2019 03:28:53

Channel:GFP set at:12-Jul-2019 03:28:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 12-Jul-2019 03:28:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 12-Jul-2019 03:28:57

Channel:Brightfield set at:12-Jul-2019 03:28:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 03:28:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:57

Channel configuration set to: GFP: 12-Jul-2019 03:28:58

Channel:GFP set at:12-Jul-2019 03:28:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 03:28:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:28:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:29:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.475

Channel configuration set to: Brightfield: 12-Jul-2019 03:29:02

Channel:Brightfield set at:12-Jul-2019 03:29:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 12-Jul-2019 03:29:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:29:03

Channel configuration set to: GFP: 12-Jul-2019 03:29:04

Channel:GFP set at:12-Jul-2019 03:29:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 12-Jul-2019 03:29:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:29:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:29:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 12-Jul-2019 03:29:08

Channel:Brightfield set at:12-Jul-2019 03:29:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 12-Jul-2019 03:29:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:29:08

Channel configuration set to: GFP: 12-Jul-2019 03:29:09

Channel:GFP set at:12-Jul-2019 03:29:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 12-Jul-2019 03:29:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:29:10

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:133 completed at:12-Jul-2019 03:29:11

Time since start of timelapse:39698.4279

PFS is locked

Time to next time point:202.252



------Time point_134------

Memory available for all arrays: 61059502080

Memory used by Matlab: 2216861696

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:32:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.8

Channel configuration set to: Brightfield: 12-Jul-2019 03:32:36

Channel:Brightfield set at:12-Jul-2019 03:32:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 03:32:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:32:36

Channel configuration set to: GFP: 12-Jul-2019 03:32:37

Channel:GFP set at:12-Jul-2019 03:32:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 03:32:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:32:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:32:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-11

Channel configuration set to: Brightfield: 12-Jul-2019 03:32:41

Channel:Brightfield set at:12-Jul-2019 03:32:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 12-Jul-2019 03:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:32:41

Channel configuration set to: GFP: 12-Jul-2019 03:32:42

Channel:GFP set at:12-Jul-2019 03:32:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 12-Jul-2019 03:32:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:32:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:32:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 12-Jul-2019 03:32:46

Channel:Brightfield set at:12-Jul-2019 03:32:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 03:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:32:47

Channel configuration set to: GFP: 12-Jul-2019 03:32:48

Channel:GFP set at:12-Jul-2019 03:32:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 03:32:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:32:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:32:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.375

Channel configuration set to: Brightfield: 12-Jul-2019 03:32:52

Channel:Brightfield set at:12-Jul-2019 03:32:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 12-Jul-2019 03:32:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:32:52

Channel configuration set to: GFP: 12-Jul-2019 03:32:54

Channel:GFP set at:12-Jul-2019 03:32:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 12-Jul-2019 03:32:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:32:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:32:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.85

Channel configuration set to: Brightfield: 12-Jul-2019 03:32:57

Channel:Brightfield set at:12-Jul-2019 03:32:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 03:32:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:32:58

Channel configuration set to: GFP: 12-Jul-2019 03:32:59

Channel:GFP set at:12-Jul-2019 03:32:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 03:32:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:32:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.2

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:03

Channel:Brightfield set at:12-Jul-2019 03:33:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 03:33:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:03

Channel configuration set to: GFP: 12-Jul-2019 03:33:05

Channel:GFP set at:12-Jul-2019 03:33:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 03:33:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:08

Channel:Brightfield set at:12-Jul-2019 03:33:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 03:33:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:08

Channel configuration set to: GFP: 12-Jul-2019 03:33:09

Channel:GFP set at:12-Jul-2019 03:33:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 03:33:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:13

Channel:Brightfield set at:12-Jul-2019 03:33:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2751. 12-Jul-2019 03:33:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:14

Channel configuration set to: GFP: 12-Jul-2019 03:33:15

Channel:GFP set at:12-Jul-2019 03:33:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2751. 12-Jul-2019 03:33:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.775

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:18

Channel:Brightfield set at:12-Jul-2019 03:33:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 03:33:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:19

Channel configuration set to: GFP: 12-Jul-2019 03:33:20

Channel:GFP set at:12-Jul-2019 03:33:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 03:33:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.425

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:24

Channel:Brightfield set at:12-Jul-2019 03:33:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 12-Jul-2019 03:33:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:24

Channel configuration set to: GFP: 12-Jul-2019 03:33:26

Channel:GFP set at:12-Jul-2019 03:33:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 12-Jul-2019 03:33:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:29

Channel:Brightfield set at:12-Jul-2019 03:33:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 03:33:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:30

Channel configuration set to: GFP: 12-Jul-2019 03:33:31

Channel:GFP set at:12-Jul-2019 03:33:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 03:33:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:34

Channel:Brightfield set at:12-Jul-2019 03:33:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 03:33:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:34

Channel configuration set to: GFP: 12-Jul-2019 03:33:36

Channel:GFP set at:12-Jul-2019 03:33:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 03:33:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:39

Channel:Brightfield set at:12-Jul-2019 03:33:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 03:33:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:40

Channel configuration set to: GFP: 12-Jul-2019 03:33:41

Channel:GFP set at:12-Jul-2019 03:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 03:33:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:45

Channel:Brightfield set at:12-Jul-2019 03:33:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 03:33:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:45

Channel configuration set to: GFP: 12-Jul-2019 03:33:47

Channel:GFP set at:12-Jul-2019 03:33:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 03:33:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:50

Channel:Brightfield set at:12-Jul-2019 03:33:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 03:33:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:50

Channel configuration set to: GFP: 12-Jul-2019 03:33:51

Channel:GFP set at:12-Jul-2019 03:33:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 03:33:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:55

Channel:Brightfield set at:12-Jul-2019 03:33:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8501. 12-Jul-2019 03:33:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:55

Channel configuration set to: GFP: 12-Jul-2019 03:33:56

Channel:GFP set at:12-Jul-2019 03:33:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 12-Jul-2019 03:33:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:33:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:33:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.775

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:34:00

Channel:Brightfield set at:12-Jul-2019 03:34:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5501. 12-Jul-2019 03:34:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:34:01

Channel configuration set to: GFP: 12-Jul-2019 03:34:02

Channel:GFP set at:12-Jul-2019 03:34:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5501. 12-Jul-2019 03:34:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:34:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:34:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 03:34:06

Channel:Brightfield set at:12-Jul-2019 03:34:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 03:34:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:34:07

Channel configuration set to: GFP: 12-Jul-2019 03:34:08

Channel:GFP set at:12-Jul-2019 03:34:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 03:34:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:34:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:134 completed at:12-Jul-2019 03:34:10

Time since start of timelapse:39997.2634

PFS is locked

Time to next time point:203.4341



------Time point_135------

Memory available for all arrays: 61059813376

Memory used by Matlab: 2217123840

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:37:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-12.15

Channel configuration set to: Brightfield: 12-Jul-2019 03:37:35

Channel:Brightfield set at:12-Jul-2019 03:37:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 03:37:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:37:35

Channel configuration set to: GFP: 12-Jul-2019 03:37:36

Channel:GFP set at:12-Jul-2019 03:37:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 03:37:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:37:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:37:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.05

Channel configuration set to: Brightfield: 12-Jul-2019 03:37:41

Channel:Brightfield set at:12-Jul-2019 03:37:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 03:37:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:37:42

Channel configuration set to: GFP: 12-Jul-2019 03:37:43

Channel:GFP set at:12-Jul-2019 03:37:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 03:37:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:37:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:37:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 12-Jul-2019 03:37:46

Channel:Brightfield set at:12-Jul-2019 03:37:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 03:37:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:37:46

Channel configuration set to: GFP: 12-Jul-2019 03:37:47

Channel:GFP set at:12-Jul-2019 03:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 03:37:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:37:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:37:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.95

Channel configuration set to: Brightfield: 12-Jul-2019 03:37:51

Channel:Brightfield set at:12-Jul-2019 03:37:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 03:37:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:37:52

Channel configuration set to: GFP: 12-Jul-2019 03:37:53

Channel:GFP set at:12-Jul-2019 03:37:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 03:37:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:37:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:37:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.9

Channel configuration set to: Brightfield: 12-Jul-2019 03:37:57

Channel:Brightfield set at:12-Jul-2019 03:37:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 03:37:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:37:57

Channel configuration set to: GFP: 12-Jul-2019 03:37:58

Channel:GFP set at:12-Jul-2019 03:37:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 03:37:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:37:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.275

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:02

Channel:Brightfield set at:12-Jul-2019 03:38:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 03:38:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:02

Channel configuration set to: GFP: 12-Jul-2019 03:38:04

Channel:GFP set at:12-Jul-2019 03:38:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 03:38:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:07

Channel:Brightfield set at:12-Jul-2019 03:38:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 03:38:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:08

Channel configuration set to: GFP: 12-Jul-2019 03:38:09

Channel:GFP set at:12-Jul-2019 03:38:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 03:38:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:12

Channel:Brightfield set at:12-Jul-2019 03:38:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 03:38:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:13

Channel configuration set to: GFP: 12-Jul-2019 03:38:14

Channel:GFP set at:12-Jul-2019 03:38:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 03:38:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.95

PFS not locked. Waiting for lock...

PFS took 0.050000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:17

Channel:Brightfield set at:12-Jul-2019 03:38:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 03:38:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:18

Channel configuration set to: GFP: 12-Jul-2019 03:38:19

Channel:GFP set at:12-Jul-2019 03:38:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 03:38:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.475

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:23

Channel:Brightfield set at:12-Jul-2019 03:38:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 03:38:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:23

Channel configuration set to: GFP: 12-Jul-2019 03:38:25

Channel:GFP set at:12-Jul-2019 03:38:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 03:38:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.925

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:29

Channel:Brightfield set at:12-Jul-2019 03:38:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2251. 12-Jul-2019 03:38:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:29

Channel configuration set to: GFP: 12-Jul-2019 03:38:30

Channel:GFP set at:12-Jul-2019 03:38:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2251. 12-Jul-2019 03:38:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:34

Channel:Brightfield set at:12-Jul-2019 03:38:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 03:38:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:34

Channel configuration set to: GFP: 12-Jul-2019 03:38:35

Channel:GFP set at:12-Jul-2019 03:38:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 03:38:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:40

Channel:Brightfield set at:12-Jul-2019 03:38:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 03:38:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:40

Channel configuration set to: GFP: 12-Jul-2019 03:38:41

Channel:GFP set at:12-Jul-2019 03:38:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 03:38:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:45

Channel:Brightfield set at:12-Jul-2019 03:38:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 03:38:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:46

Channel configuration set to: GFP: 12-Jul-2019 03:38:47

Channel:GFP set at:12-Jul-2019 03:38:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 03:38:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:50

Channel:Brightfield set at:12-Jul-2019 03:38:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 03:38:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:50

Channel configuration set to: GFP: 12-Jul-2019 03:38:52

Channel:GFP set at:12-Jul-2019 03:38:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 03:38:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 12-Jul-2019 03:38:56

Channel:Brightfield set at:12-Jul-2019 03:38:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 03:38:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:56

Channel configuration set to: GFP: 12-Jul-2019 03:38:57

Channel:GFP set at:12-Jul-2019 03:38:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 03:38:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:38:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:39:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 12-Jul-2019 03:39:01

Channel:Brightfield set at:12-Jul-2019 03:39:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 03:39:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:39:01

Channel configuration set to: GFP: 12-Jul-2019 03:39:03

Channel:GFP set at:12-Jul-2019 03:39:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 03:39:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:39:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:39:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 12-Jul-2019 03:39:06

Channel:Brightfield set at:12-Jul-2019 03:39:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 03:39:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:39:07

Channel configuration set to: GFP: 12-Jul-2019 03:39:08

Channel:GFP set at:12-Jul-2019 03:39:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 12-Jul-2019 03:39:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:39:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:135 completed at:12-Jul-2019 03:39:10

Time since start of timelapse:40297.4185

PFS is locked

Time to next time point:203.2807



------Time point_136------

Memory available for all arrays: 61061447680

Memory used by Matlab: 2217123840

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:42:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.975

Channel configuration set to: Brightfield: 12-Jul-2019 03:42:36

Channel:Brightfield set at:12-Jul-2019 03:42:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 03:42:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:42:36

Channel configuration set to: GFP: 12-Jul-2019 03:42:37

Channel:GFP set at:12-Jul-2019 03:42:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 03:42:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:42:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:42:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.225

Channel configuration set to: Brightfield: 12-Jul-2019 03:42:42

Channel:Brightfield set at:12-Jul-2019 03:42:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 03:42:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:42:42

Channel configuration set to: GFP: 12-Jul-2019 03:42:43

Channel:GFP set at:12-Jul-2019 03:42:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6251. 12-Jul-2019 03:42:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:42:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:42:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.25

Channel configuration set to: Brightfield: 12-Jul-2019 03:42:47

Channel:Brightfield set at:12-Jul-2019 03:42:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 12-Jul-2019 03:42:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:42:47

Channel configuration set to: GFP: 12-Jul-2019 03:42:48

Channel:GFP set at:12-Jul-2019 03:42:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7251. 12-Jul-2019 03:42:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:42:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:42:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.05

Channel configuration set to: Brightfield: 12-Jul-2019 03:42:52

Channel:Brightfield set at:12-Jul-2019 03:42:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 03:42:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:42:52

Channel configuration set to: GFP: 12-Jul-2019 03:42:53

Channel:GFP set at:12-Jul-2019 03:42:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 03:42:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:42:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:42:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 12-Jul-2019 03:42:57

Channel:Brightfield set at:12-Jul-2019 03:42:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 03:42:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:42:57

Channel configuration set to: GFP: 12-Jul-2019 03:42:59

Channel:GFP set at:12-Jul-2019 03:42:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 03:42:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:42:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:02

Channel:Brightfield set at:12-Jul-2019 03:43:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 12-Jul-2019 03:43:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:02

Channel configuration set to: GFP: 12-Jul-2019 03:43:04

Channel:GFP set at:12-Jul-2019 03:43:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 12-Jul-2019 03:43:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:08

Channel:Brightfield set at:12-Jul-2019 03:43:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 03:43:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:08

Channel configuration set to: GFP: 12-Jul-2019 03:43:10

Channel:GFP set at:12-Jul-2019 03:43:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 03:43:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:13

Channel:Brightfield set at:12-Jul-2019 03:43:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 03:43:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:14

Channel configuration set to: GFP: 12-Jul-2019 03:43:15

Channel:GFP set at:12-Jul-2019 03:43:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 03:43:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:18

Channel:Brightfield set at:12-Jul-2019 03:43:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 03:43:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:19

Channel configuration set to: GFP: 12-Jul-2019 03:43:20

Channel:GFP set at:12-Jul-2019 03:43:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4001. 12-Jul-2019 03:43:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:24

Channel:Brightfield set at:12-Jul-2019 03:43:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 12-Jul-2019 03:43:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:24

Channel configuration set to: GFP: 12-Jul-2019 03:43:25

Channel:GFP set at:12-Jul-2019 03:43:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2251. 12-Jul-2019 03:43:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:29

Channel:Brightfield set at:12-Jul-2019 03:43:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8501. 12-Jul-2019 03:43:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:29

Channel configuration set to: GFP: 12-Jul-2019 03:43:30

Channel:GFP set at:12-Jul-2019 03:43:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8251. 12-Jul-2019 03:43:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:34

Channel:Brightfield set at:12-Jul-2019 03:43:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 03:43:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:34

Channel configuration set to: GFP: 12-Jul-2019 03:43:35

Channel:GFP set at:12-Jul-2019 03:43:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 03:43:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:39

Channel:Brightfield set at:12-Jul-2019 03:43:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 03:43:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:39

Channel configuration set to: GFP: 12-Jul-2019 03:43:41

Channel:GFP set at:12-Jul-2019 03:43:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 03:43:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:45

Channel:Brightfield set at:12-Jul-2019 03:43:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 03:43:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:45

Channel configuration set to: GFP: 12-Jul-2019 03:43:46

Channel:GFP set at:12-Jul-2019 03:43:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 03:43:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.275

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:50

Channel:Brightfield set at:12-Jul-2019 03:43:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 03:43:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:50

Channel configuration set to: GFP: 12-Jul-2019 03:43:52

Channel:GFP set at:12-Jul-2019 03:43:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 03:43:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 12-Jul-2019 03:43:56

Channel:Brightfield set at:12-Jul-2019 03:43:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 03:43:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:56

Channel configuration set to: GFP: 12-Jul-2019 03:43:57

Channel:GFP set at:12-Jul-2019 03:43:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 03:43:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:43:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:44:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 12-Jul-2019 03:44:01

Channel:Brightfield set at:12-Jul-2019 03:44:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2751. 12-Jul-2019 03:44:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:44:01

Channel configuration set to: GFP: 12-Jul-2019 03:44:03

Channel:GFP set at:12-Jul-2019 03:44:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 12-Jul-2019 03:44:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:44:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:44:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-4.95

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:44:06

Channel:Brightfield set at:12-Jul-2019 03:44:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 03:44:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:44:07

Channel configuration set to: GFP: 12-Jul-2019 03:44:08

Channel:GFP set at:12-Jul-2019 03:44:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 12-Jul-2019 03:44:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:44:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:136 completed at:12-Jul-2019 03:44:10

Time since start of timelapse:40597.4194

PFS is locked

Time to next time point:203.2811



------Time point_137------

Memory available for all arrays: 61056712704

Memory used by Matlab: 2217185280

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:47:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.1

Channel configuration set to: Brightfield: 12-Jul-2019 03:47:36

Channel:Brightfield set at:12-Jul-2019 03:47:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 03:47:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:47:36

Channel configuration set to: GFP: 12-Jul-2019 03:47:37

Channel:GFP set at:12-Jul-2019 03:47:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 03:47:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:47:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:47:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.325

Channel configuration set to: Brightfield: 12-Jul-2019 03:47:41

Channel:Brightfield set at:12-Jul-2019 03:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 03:47:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:47:42

Channel configuration set to: GFP: 12-Jul-2019 03:47:43

Channel:GFP set at:12-Jul-2019 03:47:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 03:47:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:47:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:47:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.625

Channel configuration set to: Brightfield: 12-Jul-2019 03:47:46

Channel:Brightfield set at:12-Jul-2019 03:47:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 12-Jul-2019 03:47:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:47:47

Channel configuration set to: GFP: 12-Jul-2019 03:47:48

Channel:GFP set at:12-Jul-2019 03:47:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 12-Jul-2019 03:47:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:47:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:47:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.75

Channel configuration set to: Brightfield: 12-Jul-2019 03:47:52

Channel:Brightfield set at:12-Jul-2019 03:47:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 03:47:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:47:53

Channel configuration set to: GFP: 12-Jul-2019 03:47:54

Channel:GFP set at:12-Jul-2019 03:47:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 03:47:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:47:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:47:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 12-Jul-2019 03:47:57

Channel:Brightfield set at:12-Jul-2019 03:47:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 03:47:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:47:58

Channel configuration set to: GFP: 12-Jul-2019 03:47:59

Channel:GFP set at:12-Jul-2019 03:47:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 03:47:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:47:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.5

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:03

Channel:Brightfield set at:12-Jul-2019 03:48:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 03:48:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:03

Channel configuration set to: GFP: 12-Jul-2019 03:48:05

Channel:GFP set at:12-Jul-2019 03:48:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 03:48:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:08

Channel:Brightfield set at:12-Jul-2019 03:48:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 03:48:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:09

Channel configuration set to: GFP: 12-Jul-2019 03:48:10

Channel:GFP set at:12-Jul-2019 03:48:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 03:48:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.925

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:13

Channel:Brightfield set at:12-Jul-2019 03:48:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 03:48:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:14

Channel configuration set to: GFP: 12-Jul-2019 03:48:15

Channel:GFP set at:12-Jul-2019 03:48:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 03:48:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:18

Channel:Brightfield set at:12-Jul-2019 03:48:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 03:48:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:19

Channel configuration set to: GFP: 12-Jul-2019 03:48:20

Channel:GFP set at:12-Jul-2019 03:48:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 03:48:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:24

Channel:Brightfield set at:12-Jul-2019 03:48:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 03:48:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:24

Channel configuration set to: GFP: 12-Jul-2019 03:48:25

Channel:GFP set at:12-Jul-2019 03:48:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 03:48:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.425

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:29

Channel:Brightfield set at:12-Jul-2019 03:48:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7251. 12-Jul-2019 03:48:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:29

Channel configuration set to: GFP: 12-Jul-2019 03:48:31

Channel:GFP set at:12-Jul-2019 03:48:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7251. 12-Jul-2019 03:48:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:35

Channel:Brightfield set at:12-Jul-2019 03:48:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 03:48:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:35

Channel configuration set to: GFP: 12-Jul-2019 03:48:36

Channel:GFP set at:12-Jul-2019 03:48:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 03:48:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:40

Channel:Brightfield set at:12-Jul-2019 03:48:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 03:48:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:41

Channel configuration set to: GFP: 12-Jul-2019 03:48:42

Channel:GFP set at:12-Jul-2019 03:48:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 03:48:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:46

Channel:Brightfield set at:12-Jul-2019 03:48:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 03:48:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:46

Channel configuration set to: GFP: 12-Jul-2019 03:48:48

Channel:GFP set at:12-Jul-2019 03:48:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 03:48:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.8

PFS not locked. Waiting for lock...

PFS took 0.300000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:51

Channel:Brightfield set at:12-Jul-2019 03:48:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 03:48:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:52

Channel configuration set to: GFP: 12-Jul-2019 03:48:53

Channel:GFP set at:12-Jul-2019 03:48:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 03:48:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.975

Channel configuration set to: Brightfield: 12-Jul-2019 03:48:57

Channel:Brightfield set at:12-Jul-2019 03:48:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 03:48:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:57

Channel configuration set to: GFP: 12-Jul-2019 03:48:58

Channel:GFP set at:12-Jul-2019 03:48:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 03:48:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:48:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:49:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 12-Jul-2019 03:49:02

Channel:Brightfield set at:12-Jul-2019 03:49:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 12-Jul-2019 03:49:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:49:02

Channel configuration set to: GFP: 12-Jul-2019 03:49:04

Channel:GFP set at:12-Jul-2019 03:49:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 12-Jul-2019 03:49:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:49:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:49:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 03:49:07

Channel:Brightfield set at:12-Jul-2019 03:49:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 03:49:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:49:08

Channel configuration set to: GFP: 12-Jul-2019 03:49:09

Channel:GFP set at:12-Jul-2019 03:49:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 03:49:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:49:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:137 completed at:12-Jul-2019 03:49:11

Time since start of timelapse:40898.3495

PFS is locked

Time to next time point:202.3525



------Time point_138------

Memory available for all arrays: 61060669440

Memory used by Matlab: 2217185280

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:52:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.075

Channel configuration set to: Brightfield: 12-Jul-2019 03:52:36

Channel:Brightfield set at:12-Jul-2019 03:52:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 03:52:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:52:36

Channel configuration set to: GFP: 12-Jul-2019 03:52:37

Channel:GFP set at:12-Jul-2019 03:52:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 03:52:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:52:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:52:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.3

Channel configuration set to: Brightfield: 12-Jul-2019 03:52:41

Channel:Brightfield set at:12-Jul-2019 03:52:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 03:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:52:41

Channel configuration set to: GFP: 12-Jul-2019 03:52:43

Channel:GFP set at:12-Jul-2019 03:52:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 03:52:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:52:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:52:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 12-Jul-2019 03:52:46

Channel:Brightfield set at:12-Jul-2019 03:52:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 12-Jul-2019 03:52:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:52:47

Channel configuration set to: GFP: 12-Jul-2019 03:52:48

Channel:GFP set at:12-Jul-2019 03:52:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 12-Jul-2019 03:52:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:52:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:52:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.125

Channel configuration set to: Brightfield: 12-Jul-2019 03:52:52

Channel:Brightfield set at:12-Jul-2019 03:52:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 03:52:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:52:52

Channel configuration set to: GFP: 12-Jul-2019 03:52:53

Channel:GFP set at:12-Jul-2019 03:52:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 03:52:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:52:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:52:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 12-Jul-2019 03:52:57

Channel:Brightfield set at:12-Jul-2019 03:52:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 03:52:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:52:58

Channel configuration set to: GFP: 12-Jul-2019 03:52:59

Channel:GFP set at:12-Jul-2019 03:52:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 03:52:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:52:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:03

Channel:Brightfield set at:12-Jul-2019 03:53:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 03:53:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:03

Channel configuration set to: GFP: 12-Jul-2019 03:53:04

Channel:GFP set at:12-Jul-2019 03:53:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 03:53:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:08

Channel:Brightfield set at:12-Jul-2019 03:53:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 03:53:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:09

Channel configuration set to: GFP: 12-Jul-2019 03:53:10

Channel:GFP set at:12-Jul-2019 03:53:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 03:53:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:11

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:13

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.95

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:14

Channel:Brightfield set at:12-Jul-2019 03:53:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 03:53:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:14

Channel configuration set to: GFP: 12-Jul-2019 03:53:16

Channel:GFP set at:12-Jul-2019 03:53:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0001. 12-Jul-2019 03:53:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:19

Channel:Brightfield set at:12-Jul-2019 03:53:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4001. 12-Jul-2019 03:53:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:19

Channel configuration set to: GFP: 12-Jul-2019 03:53:20

Channel:GFP set at:12-Jul-2019 03:53:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4001. 12-Jul-2019 03:53:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:24

Channel:Brightfield set at:12-Jul-2019 03:53:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 03:53:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:24

Channel configuration set to: GFP: 12-Jul-2019 03:53:26

Channel:GFP set at:12-Jul-2019 03:53:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 03:53:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:29

Channel:Brightfield set at:12-Jul-2019 03:53:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8501. 12-Jul-2019 03:53:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:30

Channel configuration set to: GFP: 12-Jul-2019 03:53:31

Channel:GFP set at:12-Jul-2019 03:53:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8501. 12-Jul-2019 03:53:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:35

Channel:Brightfield set at:12-Jul-2019 03:53:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 03:53:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:35

Channel configuration set to: GFP: 12-Jul-2019 03:53:37

Channel:GFP set at:12-Jul-2019 03:53:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 03:53:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:40

Channel:Brightfield set at:12-Jul-2019 03:53:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 03:53:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:41

Channel configuration set to: GFP: 12-Jul-2019 03:53:42

Channel:GFP set at:12-Jul-2019 03:53:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 03:53:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:46

Channel:Brightfield set at:12-Jul-2019 03:53:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 03:53:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:46

Channel configuration set to: GFP: 12-Jul-2019 03:53:48

Channel:GFP set at:12-Jul-2019 03:53:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 03:53:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:51

Channel:Brightfield set at:12-Jul-2019 03:53:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 03:53:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:52

Channel configuration set to: GFP: 12-Jul-2019 03:53:53

Channel:GFP set at:12-Jul-2019 03:53:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 03:53:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 12-Jul-2019 03:53:57

Channel:Brightfield set at:12-Jul-2019 03:53:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 03:53:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:57

Channel configuration set to: GFP: 12-Jul-2019 03:53:58

Channel:GFP set at:12-Jul-2019 03:53:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 03:53:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:53:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:54:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.525

Channel configuration set to: Brightfield: 12-Jul-2019 03:54:02

Channel:Brightfield set at:12-Jul-2019 03:54:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 12-Jul-2019 03:54:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:54:03

Channel configuration set to: GFP: 12-Jul-2019 03:54:04

Channel:GFP set at:12-Jul-2019 03:54:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 12-Jul-2019 03:54:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:54:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:54:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.5

Channel configuration set to: Brightfield: 12-Jul-2019 03:54:08

Channel:Brightfield set at:12-Jul-2019 03:54:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 03:54:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:54:08

Channel configuration set to: GFP: 12-Jul-2019 03:54:09

Channel:GFP set at:12-Jul-2019 03:54:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 12-Jul-2019 03:54:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:54:10

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:138 completed at:12-Jul-2019 03:54:12

Time since start of timelapse:41198.6631

PFS is locked

Time to next time point:202.0403



------Time point_139------

Memory available for all arrays: 61061341184

Memory used by Matlab: 2217185280

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:57:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.55

Channel configuration set to: Brightfield: 12-Jul-2019 03:57:35

Channel:Brightfield set at:12-Jul-2019 03:57:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 03:57:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:57:35

Channel configuration set to: GFP: 12-Jul-2019 03:57:37

Channel:GFP set at:12-Jul-2019 03:57:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 03:57:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:57:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:57:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 12-Jul-2019 03:57:40

Channel:Brightfield set at:12-Jul-2019 03:57:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 03:57:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:57:41

Channel configuration set to: GFP: 12-Jul-2019 03:57:42

Channel:GFP set at:12-Jul-2019 03:57:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 03:57:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:57:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:57:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.475

Channel configuration set to: Brightfield: 12-Jul-2019 03:57:46

Channel:Brightfield set at:12-Jul-2019 03:57:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 03:57:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:57:46

Channel configuration set to: GFP: 12-Jul-2019 03:57:48

Channel:GFP set at:12-Jul-2019 03:57:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 03:57:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:57:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:57:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.225

Channel configuration set to: Brightfield: 12-Jul-2019 03:57:51

Channel:Brightfield set at:12-Jul-2019 03:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 03:57:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:57:52

Channel configuration set to: GFP: 12-Jul-2019 03:57:53

Channel:GFP set at:12-Jul-2019 03:57:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 03:57:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:57:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:57:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 12-Jul-2019 03:57:57

Channel:Brightfield set at:12-Jul-2019 03:57:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 03:57:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:57:57

Channel configuration set to: GFP: 12-Jul-2019 03:57:58

Channel:GFP set at:12-Jul-2019 03:57:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 03:57:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:57:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.575

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:02

Channel:Brightfield set at:12-Jul-2019 03:58:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 03:58:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:03

Channel configuration set to: GFP: 12-Jul-2019 03:58:04

Channel:GFP set at:12-Jul-2019 03:58:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 03:58:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:08

Channel:Brightfield set at:12-Jul-2019 03:58:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 03:58:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:08

Channel configuration set to: GFP: 12-Jul-2019 03:58:09

Channel:GFP set at:12-Jul-2019 03:58:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 03:58:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.925

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:13

Channel:Brightfield set at:12-Jul-2019 03:58:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 03:58:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:13

Channel configuration set to: GFP: 12-Jul-2019 03:58:14

Channel:GFP set at:12-Jul-2019 03:58:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 03:58:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:18

Channel:Brightfield set at:12-Jul-2019 03:58:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 03:58:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:18

Channel configuration set to: GFP: 12-Jul-2019 03:58:20

Channel:GFP set at:12-Jul-2019 03:58:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 03:58:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:23

Channel:Brightfield set at:12-Jul-2019 03:58:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 03:58:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:24

Channel configuration set to: GFP: 12-Jul-2019 03:58:25

Channel:GFP set at:12-Jul-2019 03:58:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 03:58:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.475

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:28

Channel:Brightfield set at:12-Jul-2019 03:58:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6751. 12-Jul-2019 03:58:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:29

Channel configuration set to: GFP: 12-Jul-2019 03:58:30

Channel:GFP set at:12-Jul-2019 03:58:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6751. 12-Jul-2019 03:58:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:34

Channel:Brightfield set at:12-Jul-2019 03:58:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 03:58:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:35

Channel configuration set to: GFP: 12-Jul-2019 03:58:36

Channel:GFP set at:12-Jul-2019 03:58:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 03:58:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:40

Channel:Brightfield set at:12-Jul-2019 03:58:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 03:58:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:40

Channel configuration set to: GFP: 12-Jul-2019 03:58:42

Channel:GFP set at:12-Jul-2019 03:58:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 03:58:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:45

Channel:Brightfield set at:12-Jul-2019 03:58:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 03:58:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:45

Channel configuration set to: GFP: 12-Jul-2019 03:58:47

Channel:GFP set at:12-Jul-2019 03:58:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 03:58:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:50

Channel:Brightfield set at:12-Jul-2019 03:58:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 03:58:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:51

Channel configuration set to: GFP: 12-Jul-2019 03:58:52

Channel:GFP set at:12-Jul-2019 03:58:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 03:58:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 03:58:56

Channel:Brightfield set at:12-Jul-2019 03:58:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 12-Jul-2019 03:58:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:56

Channel configuration set to: GFP: 12-Jul-2019 03:58:57

Channel:GFP set at:12-Jul-2019 03:58:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3751. 12-Jul-2019 03:58:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:58:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:59:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 12-Jul-2019 03:59:01

Channel:Brightfield set at:12-Jul-2019 03:59:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 12-Jul-2019 03:59:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:59:02

Channel configuration set to: GFP: 12-Jul-2019 03:59:03

Channel:GFP set at:12-Jul-2019 03:59:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 12-Jul-2019 03:59:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:59:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 03:59:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.625

Channel configuration set to: Brightfield: 12-Jul-2019 03:59:06

Channel:Brightfield set at:12-Jul-2019 03:59:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 03:59:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:59:07

Channel configuration set to: GFP: 12-Jul-2019 03:59:08

Channel:GFP set at:12-Jul-2019 03:59:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7001. 12-Jul-2019 03:59:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 03:59:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:139 completed at:12-Jul-2019 03:59:10

Time since start of timelapse:41497.202

PFS is locked

Time to next time point:203.5027



------Time point_140------

Memory available for all arrays: 61055152128

Memory used by Matlab: 2217508864

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:02:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.125

Channel configuration set to: Brightfield: 12-Jul-2019 04:02:36

Channel:Brightfield set at:12-Jul-2019 04:02:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 04:02:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:02:36

Channel configuration set to: GFP: 12-Jul-2019 04:02:37

Channel:GFP set at:12-Jul-2019 04:02:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 04:02:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:02:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:02:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 12-Jul-2019 04:02:41

Channel:Brightfield set at:12-Jul-2019 04:02:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 04:02:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:02:41

Channel configuration set to: GFP: 12-Jul-2019 04:02:43

Channel:GFP set at:12-Jul-2019 04:02:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 04:02:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:02:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:02:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 12-Jul-2019 04:02:46

Channel:Brightfield set at:12-Jul-2019 04:02:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4501. 12-Jul-2019 04:02:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:02:46

Channel configuration set to: GFP: 12-Jul-2019 04:02:47

Channel:GFP set at:12-Jul-2019 04:02:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4501. 12-Jul-2019 04:02:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:02:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:02:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.125

Channel configuration set to: Brightfield: 12-Jul-2019 04:02:51

Channel:Brightfield set at:12-Jul-2019 04:02:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 04:02:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:02:51

Channel configuration set to: GFP: 12-Jul-2019 04:02:53

Channel:GFP set at:12-Jul-2019 04:02:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 04:02:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:02:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:02:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 12-Jul-2019 04:02:56

Channel:Brightfield set at:12-Jul-2019 04:02:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 04:02:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:02:56

Channel configuration set to: GFP: 12-Jul-2019 04:02:58

Channel:GFP set at:12-Jul-2019 04:02:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 04:02:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:02:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.5

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:01

Channel:Brightfield set at:12-Jul-2019 04:03:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 12-Jul-2019 04:03:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:02

Channel configuration set to: GFP: 12-Jul-2019 04:03:03

Channel:GFP set at:12-Jul-2019 04:03:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6751. 12-Jul-2019 04:03:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:07

Channel:Brightfield set at:12-Jul-2019 04:03:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 04:03:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:07

Channel configuration set to: GFP: 12-Jul-2019 04:03:08

Channel:GFP set at:12-Jul-2019 04:03:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 04:03:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:12

Channel:Brightfield set at:12-Jul-2019 04:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 04:03:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:13

Channel configuration set to: GFP: 12-Jul-2019 04:03:14

Channel:GFP set at:12-Jul-2019 04:03:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 12-Jul-2019 04:03:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:17

Channel:Brightfield set at:12-Jul-2019 04:03:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 12-Jul-2019 04:03:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:18

Channel configuration set to: GFP: 12-Jul-2019 04:03:19

Channel:GFP set at:12-Jul-2019 04:03:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 12-Jul-2019 04:03:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:23

Channel:Brightfield set at:12-Jul-2019 04:03:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 04:03:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:23

Channel configuration set to: GFP: 12-Jul-2019 04:03:25

Channel:GFP set at:12-Jul-2019 04:03:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 04:03:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:28

Channel:Brightfield set at:12-Jul-2019 04:03:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7501. 12-Jul-2019 04:03:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:29

Channel configuration set to: GFP: 12-Jul-2019 04:03:30

Channel:GFP set at:12-Jul-2019 04:03:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7501. 12-Jul-2019 04:03:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:33

Channel:Brightfield set at:12-Jul-2019 04:03:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 04:03:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:34

Channel configuration set to: GFP: 12-Jul-2019 04:03:35

Channel:GFP set at:12-Jul-2019 04:03:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 04:03:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:39

Channel:Brightfield set at:12-Jul-2019 04:03:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 04:03:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:39

Channel configuration set to: GFP: 12-Jul-2019 04:03:41

Channel:GFP set at:12-Jul-2019 04:03:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 04:03:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:45

Channel:Brightfield set at:12-Jul-2019 04:03:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 04:03:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:45

Channel configuration set to: GFP: 12-Jul-2019 04:03:46

Channel:GFP set at:12-Jul-2019 04:03:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 04:03:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:50

Channel:Brightfield set at:12-Jul-2019 04:03:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 04:03:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:50

Channel configuration set to: GFP: 12-Jul-2019 04:03:51

Channel:GFP set at:12-Jul-2019 04:03:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 04:03:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:55

Channel:Brightfield set at:12-Jul-2019 04:03:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5501. 12-Jul-2019 04:03:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:55

Channel configuration set to: GFP: 12-Jul-2019 04:03:56

Channel:GFP set at:12-Jul-2019 04:03:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5751. 12-Jul-2019 04:03:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:03:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 04:03:59

Channel:Brightfield set at:12-Jul-2019 04:03:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 04:04:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:04:00

Channel configuration set to: GFP: 12-Jul-2019 04:04:01

Channel:GFP set at:12-Jul-2019 04:04:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 04:04:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:04:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:04:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 04:04:06

Channel:Brightfield set at:12-Jul-2019 04:04:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 12-Jul-2019 04:04:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:04:06

Channel configuration set to: GFP: 12-Jul-2019 04:04:07

Channel:GFP set at:12-Jul-2019 04:04:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 12-Jul-2019 04:04:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:04:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:140 completed at:12-Jul-2019 04:04:10

Time since start of timelapse:41796.8014

PFS is locked

Time to next time point:203.9047



------Time point_141------

Memory available for all arrays: 61060599808

Memory used by Matlab: 2217771008

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:07:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.075

Channel configuration set to: Brightfield: 12-Jul-2019 04:07:35

Channel:Brightfield set at:12-Jul-2019 04:07:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 04:07:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:07:35

Channel configuration set to: GFP: 12-Jul-2019 04:07:37

Channel:GFP set at:12-Jul-2019 04:07:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 12-Jul-2019 04:07:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:07:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:07:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.05

Channel configuration set to: Brightfield: 12-Jul-2019 04:07:41

Channel:Brightfield set at:12-Jul-2019 04:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 12-Jul-2019 04:07:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:07:41

Channel configuration set to: GFP: 12-Jul-2019 04:07:42

Channel:GFP set at:12-Jul-2019 04:07:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 04:07:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:07:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:07:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 12-Jul-2019 04:07:45

Channel:Brightfield set at:12-Jul-2019 04:07:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 04:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:07:46

Channel configuration set to: GFP: 12-Jul-2019 04:07:47

Channel:GFP set at:12-Jul-2019 04:07:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 04:07:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:07:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:07:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.95

Channel configuration set to: Brightfield: 12-Jul-2019 04:07:51

Channel:Brightfield set at:12-Jul-2019 04:07:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 04:07:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:07:51

Channel configuration set to: GFP: 12-Jul-2019 04:07:52

Channel:GFP set at:12-Jul-2019 04:07:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 04:07:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:07:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:07:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 12-Jul-2019 04:07:56

Channel:Brightfield set at:12-Jul-2019 04:07:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 04:07:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:07:56

Channel configuration set to: GFP: 12-Jul-2019 04:07:57

Channel:GFP set at:12-Jul-2019 04:07:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 04:07:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:07:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.35

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:01

Channel:Brightfield set at:12-Jul-2019 04:08:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 12-Jul-2019 04:08:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:01

Channel configuration set to: GFP: 12-Jul-2019 04:08:02

Channel:GFP set at:12-Jul-2019 04:08:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 04:08:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:06

Channel:Brightfield set at:12-Jul-2019 04:08:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 04:08:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:06

Channel configuration set to: GFP: 12-Jul-2019 04:08:07

Channel:GFP set at:12-Jul-2019 04:08:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 04:08:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:11

Channel:Brightfield set at:12-Jul-2019 04:08:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 04:08:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:11

Channel configuration set to: GFP: 12-Jul-2019 04:08:12

Channel:GFP set at:12-Jul-2019 04:08:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 04:08:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:13

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:15

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:16

Channel:Brightfield set at:12-Jul-2019 04:08:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 12-Jul-2019 04:08:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:16

Channel configuration set to: GFP: 12-Jul-2019 04:08:17

Channel:GFP set at:12-Jul-2019 04:08:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 12-Jul-2019 04:08:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:18

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:20

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:21

Channel:Brightfield set at:12-Jul-2019 04:08:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 12-Jul-2019 04:08:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:22

Channel configuration set to: GFP: 12-Jul-2019 04:08:23

Channel:GFP set at:12-Jul-2019 04:08:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 12-Jul-2019 04:08:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:23

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:25

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.325

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:26

Channel:Brightfield set at:12-Jul-2019 04:08:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8751. 12-Jul-2019 04:08:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:27

Channel configuration set to: GFP: 12-Jul-2019 04:08:28

Channel:GFP set at:12-Jul-2019 04:08:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8501. 12-Jul-2019 04:08:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:28

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:31

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:32

Channel:Brightfield set at:12-Jul-2019 04:08:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 04:08:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:32

Channel configuration set to: GFP: 12-Jul-2019 04:08:33

Channel:GFP set at:12-Jul-2019 04:08:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 04:08:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:34

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:36

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.95

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:37

Channel:Brightfield set at:12-Jul-2019 04:08:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 04:08:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:37

Channel configuration set to: GFP: 12-Jul-2019 04:08:38

Channel:GFP set at:12-Jul-2019 04:08:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 04:08:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:39

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:41

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:42

Channel:Brightfield set at:12-Jul-2019 04:08:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 04:08:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:43

Channel configuration set to: GFP: 12-Jul-2019 04:08:44

Channel:GFP set at:12-Jul-2019 04:08:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 04:08:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:44

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:47

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:48

Channel:Brightfield set at:12-Jul-2019 04:08:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 04:08:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:48

Channel configuration set to: GFP: 12-Jul-2019 04:08:49

Channel:GFP set at:12-Jul-2019 04:08:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 04:08:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:50

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:52

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:53

Channel:Brightfield set at:12-Jul-2019 04:08:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 04:08:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:54

Channel configuration set to: GFP: 12-Jul-2019 04:08:55

Channel:GFP set at:12-Jul-2019 04:08:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 04:08:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:55

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:57

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.225

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 04:08:58

Channel:Brightfield set at:12-Jul-2019 04:08:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 04:08:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:08:59

Channel configuration set to: GFP: 12-Jul-2019 04:09:00

Channel:GFP set at:12-Jul-2019 04:09:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 04:09:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:09:00

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:09:02

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 12-Jul-2019 04:09:04

Channel:Brightfield set at:12-Jul-2019 04:09:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 04:09:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:09:04

Channel configuration set to: GFP: 12-Jul-2019 04:09:05

Channel:GFP set at:12-Jul-2019 04:09:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 04:09:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:09:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:141 completed at:12-Jul-2019 04:09:08

Time since start of timelapse:42094.9278

PFS is locked

Time to next time point:205.7799



------Time point_142------

Memory available for all arrays: 61044174848

Memory used by Matlab: 2217934848

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:12:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.85

Channel configuration set to: Brightfield: 12-Jul-2019 04:12:36

Channel:Brightfield set at:12-Jul-2019 04:12:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:12:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:12:36

Channel configuration set to: GFP: 12-Jul-2019 04:12:37

Channel:GFP set at:12-Jul-2019 04:12:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:12:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:12:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:12:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.05

Channel configuration set to: Brightfield: 12-Jul-2019 04:12:42

Channel:Brightfield set at:12-Jul-2019 04:12:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 04:12:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:12:42

Channel configuration set to: GFP: 12-Jul-2019 04:12:43

Channel:GFP set at:12-Jul-2019 04:12:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 04:12:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:12:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:12:46

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 12-Jul-2019 04:12:47

Channel:Brightfield set at:12-Jul-2019 04:12:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 04:12:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:12:47

Channel configuration set to: GFP: 12-Jul-2019 04:12:48

Channel:GFP set at:12-Jul-2019 04:12:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 04:12:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:12:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:12:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.975

Channel configuration set to: Brightfield: 12-Jul-2019 04:12:52

Channel:Brightfield set at:12-Jul-2019 04:12:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 04:12:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:12:52

Channel configuration set to: GFP: 12-Jul-2019 04:12:54

Channel:GFP set at:12-Jul-2019 04:12:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 04:12:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:12:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:12:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.025

Channel configuration set to: Brightfield: 12-Jul-2019 04:12:57

Channel:Brightfield set at:12-Jul-2019 04:12:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 04:12:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:12:58

Channel configuration set to: GFP: 12-Jul-2019 04:12:59

Channel:GFP set at:12-Jul-2019 04:12:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 04:13:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:00

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:03

Channel:Brightfield set at:12-Jul-2019 04:13:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 12-Jul-2019 04:13:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:03

Channel configuration set to: GFP: 12-Jul-2019 04:13:05

Channel:GFP set at:12-Jul-2019 04:13:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 04:13:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.925

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:08

Channel:Brightfield set at:12-Jul-2019 04:13:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 04:13:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:08

Channel configuration set to: GFP: 12-Jul-2019 04:13:09

Channel:GFP set at:12-Jul-2019 04:13:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 04:13:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.775

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:13

Channel:Brightfield set at:12-Jul-2019 04:13:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 04:13:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:14

Channel configuration set to: GFP: 12-Jul-2019 04:13:15

Channel:GFP set at:12-Jul-2019 04:13:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 04:13:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.075

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:19

Channel:Brightfield set at:12-Jul-2019 04:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 12-Jul-2019 04:13:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:19

Channel configuration set to: GFP: 12-Jul-2019 04:13:21

Channel:GFP set at:12-Jul-2019 04:13:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 04:13:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:24

Channel:Brightfield set at:12-Jul-2019 04:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 04:13:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:25

Channel configuration set to: GFP: 12-Jul-2019 04:13:26

Channel:GFP set at:12-Jul-2019 04:13:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 04:13:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:29

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.25

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:30

Channel:Brightfield set at:12-Jul-2019 04:13:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 04:13:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:30

Channel configuration set to: GFP: 12-Jul-2019 04:13:31

Channel:GFP set at:12-Jul-2019 04:13:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 04:13:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:35

Channel:Brightfield set at:12-Jul-2019 04:13:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 04:13:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:35

Channel configuration set to: GFP: 12-Jul-2019 04:13:36

Channel:GFP set at:12-Jul-2019 04:13:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 04:13:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:41

Channel:Brightfield set at:12-Jul-2019 04:13:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 04:13:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:41

Channel configuration set to: GFP: 12-Jul-2019 04:13:42

Channel:GFP set at:12-Jul-2019 04:13:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 04:13:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:46

Channel:Brightfield set at:12-Jul-2019 04:13:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 04:13:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:47

Channel configuration set to: GFP: 12-Jul-2019 04:13:48

Channel:GFP set at:12-Jul-2019 04:13:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 04:13:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:51

Channel:Brightfield set at:12-Jul-2019 04:13:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:13:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:51

Channel configuration set to: GFP: 12-Jul-2019 04:13:53

Channel:GFP set at:12-Jul-2019 04:13:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:13:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 12-Jul-2019 04:13:57

Channel:Brightfield set at:12-Jul-2019 04:13:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 04:13:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:57

Channel configuration set to: GFP: 12-Jul-2019 04:13:58

Channel:GFP set at:12-Jul-2019 04:13:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 04:13:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:13:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:14:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 12-Jul-2019 04:14:02

Channel:Brightfield set at:12-Jul-2019 04:14:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 12-Jul-2019 04:14:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:14:02

Channel configuration set to: GFP: 12-Jul-2019 04:14:03

Channel:GFP set at:12-Jul-2019 04:14:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 12-Jul-2019 04:14:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:14:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:14:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.425

Channel configuration set to: Brightfield: 12-Jul-2019 04:14:07

Channel:Brightfield set at:12-Jul-2019 04:14:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 04:14:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:14:07

Channel configuration set to: GFP: 12-Jul-2019 04:14:09

Channel:GFP set at:12-Jul-2019 04:14:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 04:14:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:14:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:142 completed at:12-Jul-2019 04:14:11

Time since start of timelapse:42398.0932

PFS is locked

Time to next time point:202.6162



------Time point_143------

Memory available for all arrays: 61065809920

Memory used by Matlab: 2217996288

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:17:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.05

Channel configuration set to: Brightfield: 12-Jul-2019 04:17:35

Channel:Brightfield set at:12-Jul-2019 04:17:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 04:17:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:17:36

Channel configuration set to: GFP: 12-Jul-2019 04:17:37

Channel:GFP set at:12-Jul-2019 04:17:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 04:17:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:17:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:17:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 04:17:41

Channel:Brightfield set at:12-Jul-2019 04:17:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6751. 12-Jul-2019 04:17:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:17:41

Channel configuration set to: GFP: 12-Jul-2019 04:17:43

Channel:GFP set at:12-Jul-2019 04:17:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6751. 12-Jul-2019 04:17:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:17:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:17:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 12-Jul-2019 04:17:46

Channel:Brightfield set at:12-Jul-2019 04:17:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 04:17:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:17:46

Channel configuration set to: GFP: 12-Jul-2019 04:17:47

Channel:GFP set at:12-Jul-2019 04:17:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 04:17:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:17:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:17:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.05

Channel configuration set to: Brightfield: 12-Jul-2019 04:17:51

Channel:Brightfield set at:12-Jul-2019 04:17:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 04:17:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:17:51

Channel configuration set to: GFP: 12-Jul-2019 04:17:52

Channel:GFP set at:12-Jul-2019 04:17:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 04:17:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:17:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:17:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 12-Jul-2019 04:17:56

Channel:Brightfield set at:12-Jul-2019 04:17:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 04:17:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:17:57

Channel configuration set to: GFP: 12-Jul-2019 04:17:58

Channel:GFP set at:12-Jul-2019 04:17:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 04:17:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:17:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:02

Channel:Brightfield set at:12-Jul-2019 04:18:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 04:18:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:02

Channel configuration set to: GFP: 12-Jul-2019 04:18:03

Channel:GFP set at:12-Jul-2019 04:18:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 04:18:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:08

Channel:Brightfield set at:12-Jul-2019 04:18:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 04:18:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:08

Channel configuration set to: GFP: 12-Jul-2019 04:18:09

Channel:GFP set at:12-Jul-2019 04:18:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 04:18:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:13

Channel:Brightfield set at:12-Jul-2019 04:18:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 04:18:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:13

Channel configuration set to: GFP: 12-Jul-2019 04:18:14

Channel:GFP set at:12-Jul-2019 04:18:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 04:18:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:18

Channel:Brightfield set at:12-Jul-2019 04:18:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 04:18:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:19

Channel configuration set to: GFP: 12-Jul-2019 04:18:20

Channel:GFP set at:12-Jul-2019 04:18:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 04:18:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:24

Channel:Brightfield set at:12-Jul-2019 04:18:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2251. 12-Jul-2019 04:18:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:24

Channel configuration set to: GFP: 12-Jul-2019 04:18:25

Channel:GFP set at:12-Jul-2019 04:18:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2251. 12-Jul-2019 04:18:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:29

Channel:Brightfield set at:12-Jul-2019 04:18:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8501. 12-Jul-2019 04:18:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:29

Channel configuration set to: GFP: 12-Jul-2019 04:18:31

Channel:GFP set at:12-Jul-2019 04:18:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8501. 12-Jul-2019 04:18:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:35

Channel:Brightfield set at:12-Jul-2019 04:18:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 04:18:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:35

Channel configuration set to: GFP: 12-Jul-2019 04:18:36

Channel:GFP set at:12-Jul-2019 04:18:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 04:18:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:41

Channel:Brightfield set at:12-Jul-2019 04:18:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 04:18:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:41

Channel configuration set to: GFP: 12-Jul-2019 04:18:42

Channel:GFP set at:12-Jul-2019 04:18:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 04:18:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:46

Channel:Brightfield set at:12-Jul-2019 04:18:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 12-Jul-2019 04:18:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:46

Channel configuration set to: GFP: 12-Jul-2019 04:18:48

Channel:GFP set at:12-Jul-2019 04:18:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 12-Jul-2019 04:18:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.275

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:51

Channel:Brightfield set at:12-Jul-2019 04:18:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 12-Jul-2019 04:18:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:52

Channel configuration set to: GFP: 12-Jul-2019 04:18:53

Channel:GFP set at:12-Jul-2019 04:18:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 04:18:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:54

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 12-Jul-2019 04:18:57

Channel:Brightfield set at:12-Jul-2019 04:18:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 04:18:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:57

Channel configuration set to: GFP: 12-Jul-2019 04:18:59

Channel:GFP set at:12-Jul-2019 04:18:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 04:18:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:18:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:19:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.475

Channel configuration set to: Brightfield: 12-Jul-2019 04:19:02

Channel:Brightfield set at:12-Jul-2019 04:19:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2751. 12-Jul-2019 04:19:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:19:02

Channel configuration set to: GFP: 12-Jul-2019 04:19:04

Channel:GFP set at:12-Jul-2019 04:19:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 12-Jul-2019 04:19:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:19:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:19:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 12-Jul-2019 04:19:07

Channel:Brightfield set at:12-Jul-2019 04:19:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 04:19:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:19:08

Channel configuration set to: GFP: 12-Jul-2019 04:19:09

Channel:GFP set at:12-Jul-2019 04:19:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 04:19:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:19:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:143 completed at:12-Jul-2019 04:19:11

Time since start of timelapse:42698.4353

PFS is locked

Time to next time point:202.2754



------Time point_144------

Memory available for all arrays: 61062426624

Memory used by Matlab: 2217996288

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:22:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-12.1

Channel configuration set to: Brightfield: 12-Jul-2019 04:22:35

Channel:Brightfield set at:12-Jul-2019 04:22:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 04:22:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:22:35

Channel configuration set to: GFP: 12-Jul-2019 04:22:36

Channel:GFP set at:12-Jul-2019 04:22:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 04:22:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:22:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:22:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 04:22:40

Channel:Brightfield set at:12-Jul-2019 04:22:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 04:22:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:22:41

Channel configuration set to: GFP: 12-Jul-2019 04:22:42

Channel:GFP set at:12-Jul-2019 04:22:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 04:22:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:22:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:22:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.475

Channel configuration set to: Brightfield: 12-Jul-2019 04:22:45

Channel:Brightfield set at:12-Jul-2019 04:22:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 12-Jul-2019 04:22:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:22:45

Channel configuration set to: GFP: 12-Jul-2019 04:22:46

Channel:GFP set at:12-Jul-2019 04:22:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 12-Jul-2019 04:22:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:22:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:22:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.175

Channel configuration set to: Brightfield: 12-Jul-2019 04:22:50

Channel:Brightfield set at:12-Jul-2019 04:22:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 04:22:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:22:51

Channel configuration set to: GFP: 12-Jul-2019 04:22:52

Channel:GFP set at:12-Jul-2019 04:22:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 04:22:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:22:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:22:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.175

Channel configuration set to: Brightfield: 12-Jul-2019 04:22:56

Channel:Brightfield set at:12-Jul-2019 04:22:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 04:22:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:22:56

Channel configuration set to: GFP: 12-Jul-2019 04:22:57

Channel:GFP set at:12-Jul-2019 04:22:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 04:22:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:22:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.575

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:01

Channel:Brightfield set at:12-Jul-2019 04:23:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 12-Jul-2019 04:23:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:02

Channel configuration set to: GFP: 12-Jul-2019 04:23:03

Channel:GFP set at:12-Jul-2019 04:23:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 12-Jul-2019 04:23:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:07

Channel:Brightfield set at:12-Jul-2019 04:23:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 04:23:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:07

Channel configuration set to: GFP: 12-Jul-2019 04:23:09

Channel:GFP set at:12-Jul-2019 04:23:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 04:23:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:12

Channel:Brightfield set at:12-Jul-2019 04:23:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 04:23:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:13

Channel configuration set to: GFP: 12-Jul-2019 04:23:14

Channel:GFP set at:12-Jul-2019 04:23:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 04:23:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:18

Channel:Brightfield set at:12-Jul-2019 04:23:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 04:23:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:18

Channel configuration set to: GFP: 12-Jul-2019 04:23:19

Channel:GFP set at:12-Jul-2019 04:23:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 04:23:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:23

Channel:Brightfield set at:12-Jul-2019 04:23:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 04:23:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:24

Channel configuration set to: GFP: 12-Jul-2019 04:23:25

Channel:GFP set at:12-Jul-2019 04:23:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2501. 12-Jul-2019 04:23:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:28

Channel:Brightfield set at:12-Jul-2019 04:23:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7501. 12-Jul-2019 04:23:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:29

Channel configuration set to: GFP: 12-Jul-2019 04:23:30

Channel:GFP set at:12-Jul-2019 04:23:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7251. 12-Jul-2019 04:23:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:34

Channel:Brightfield set at:12-Jul-2019 04:23:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 04:23:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:34

Channel configuration set to: GFP: 12-Jul-2019 04:23:36

Channel:GFP set at:12-Jul-2019 04:23:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 04:23:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:40

Channel:Brightfield set at:12-Jul-2019 04:23:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 04:23:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:40

Channel configuration set to: GFP: 12-Jul-2019 04:23:41

Channel:GFP set at:12-Jul-2019 04:23:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 04:23:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:45

Channel:Brightfield set at:12-Jul-2019 04:23:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 04:23:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:46

Channel configuration set to: GFP: 12-Jul-2019 04:23:47

Channel:GFP set at:12-Jul-2019 04:23:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 04:23:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:50

Channel:Brightfield set at:12-Jul-2019 04:23:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 04:23:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:51

Channel configuration set to: GFP: 12-Jul-2019 04:23:52

Channel:GFP set at:12-Jul-2019 04:23:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 04:23:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.95

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:55

Channel:Brightfield set at:12-Jul-2019 04:23:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 12-Jul-2019 04:23:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:56

Channel configuration set to: GFP: 12-Jul-2019 04:23:57

Channel:GFP set at:12-Jul-2019 04:23:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 12-Jul-2019 04:23:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:23:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:23:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.45

Channel configuration set to: Brightfield: 12-Jul-2019 04:24:00

Channel:Brightfield set at:12-Jul-2019 04:24:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 12-Jul-2019 04:24:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:24:01

Channel configuration set to: GFP: 12-Jul-2019 04:24:02

Channel:GFP set at:12-Jul-2019 04:24:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2751. 12-Jul-2019 04:24:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:24:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:24:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 12-Jul-2019 04:24:06

Channel:Brightfield set at:12-Jul-2019 04:24:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 04:24:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:24:07

Channel configuration set to: GFP: 12-Jul-2019 04:24:08

Channel:GFP set at:12-Jul-2019 04:24:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 04:24:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:24:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:144 completed at:12-Jul-2019 04:24:10

Time since start of timelapse:42997.2673

PFS is locked

Time to next time point:203.4446



------Time point_145------

Memory available for all arrays: 61062975488

Memory used by Matlab: 2214547456

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:27:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.025

Channel configuration set to: Brightfield: 12-Jul-2019 04:27:35

Channel:Brightfield set at:12-Jul-2019 04:27:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 04:27:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:27:36

Channel configuration set to: GFP: 12-Jul-2019 04:27:37

Channel:GFP set at:12-Jul-2019 04:27:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 04:27:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:27:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:27:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 04:27:41

Channel:Brightfield set at:12-Jul-2019 04:27:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 04:27:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:27:41

Channel configuration set to: GFP: 12-Jul-2019 04:27:43

Channel:GFP set at:12-Jul-2019 04:27:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 04:27:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:27:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:27:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 12-Jul-2019 04:27:46

Channel:Brightfield set at:12-Jul-2019 04:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 04:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:27:46

Channel configuration set to: GFP: 12-Jul-2019 04:27:47

Channel:GFP set at:12-Jul-2019 04:27:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 04:27:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:27:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:27:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.075

Channel configuration set to: Brightfield: 12-Jul-2019 04:27:51

Channel:Brightfield set at:12-Jul-2019 04:27:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 04:27:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:27:51

Channel configuration set to: GFP: 12-Jul-2019 04:27:53

Channel:GFP set at:12-Jul-2019 04:27:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 04:27:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:27:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:27:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.175

Channel configuration set to: Brightfield: 12-Jul-2019 04:27:56

Channel:Brightfield set at:12-Jul-2019 04:27:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 04:27:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:27:57

Channel configuration set to: GFP: 12-Jul-2019 04:27:58

Channel:GFP set at:12-Jul-2019 04:27:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 04:27:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:27:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.425

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:02

Channel:Brightfield set at:12-Jul-2019 04:28:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 04:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:02

Channel configuration set to: GFP: 12-Jul-2019 04:28:03

Channel:GFP set at:12-Jul-2019 04:28:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 04:28:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:08

Channel:Brightfield set at:12-Jul-2019 04:28:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 12-Jul-2019 04:28:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:08

Channel configuration set to: GFP: 12-Jul-2019 04:28:10

Channel:GFP set at:12-Jul-2019 04:28:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 12-Jul-2019 04:28:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:13

Channel:Brightfield set at:12-Jul-2019 04:28:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 04:28:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:13

Channel configuration set to: GFP: 12-Jul-2019 04:28:15

Channel:GFP set at:12-Jul-2019 04:28:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 04:28:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:18

Channel:Brightfield set at:12-Jul-2019 04:28:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 04:28:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:18

Channel configuration set to: GFP: 12-Jul-2019 04:28:20

Channel:GFP set at:12-Jul-2019 04:28:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 04:28:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:23

Channel:Brightfield set at:12-Jul-2019 04:28:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 12-Jul-2019 04:28:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:24

Channel configuration set to: GFP: 12-Jul-2019 04:28:25

Channel:GFP set at:12-Jul-2019 04:28:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 12-Jul-2019 04:28:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:29

Channel:Brightfield set at:12-Jul-2019 04:28:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 04:28:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:30

Channel configuration set to: GFP: 12-Jul-2019 04:28:31

Channel:GFP set at:12-Jul-2019 04:28:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 04:28:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:35

Channel:Brightfield set at:12-Jul-2019 04:28:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 12-Jul-2019 04:28:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:35

Channel configuration set to: GFP: 12-Jul-2019 04:28:37

Channel:GFP set at:12-Jul-2019 04:28:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 12-Jul-2019 04:28:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:41

Channel:Brightfield set at:12-Jul-2019 04:28:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 04:28:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:41

Channel configuration set to: GFP: 12-Jul-2019 04:28:43

Channel:GFP set at:12-Jul-2019 04:28:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 04:28:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:47

Channel:Brightfield set at:12-Jul-2019 04:28:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:28:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:47

Channel configuration set to: GFP: 12-Jul-2019 04:28:48

Channel:GFP set at:12-Jul-2019 04:28:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:28:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:49

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:51

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:52

Channel:Brightfield set at:12-Jul-2019 04:28:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 04:28:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:53

Channel configuration set to: GFP: 12-Jul-2019 04:28:54

Channel:GFP set at:12-Jul-2019 04:28:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 04:28:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:54

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:57

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 12-Jul-2019 04:28:58

Channel:Brightfield set at:12-Jul-2019 04:28:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 04:28:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:28:58

Channel configuration set to: GFP: 12-Jul-2019 04:28:59

Channel:GFP set at:12-Jul-2019 04:28:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 04:29:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:29:00

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:29:02

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 12-Jul-2019 04:29:03

Channel:Brightfield set at:12-Jul-2019 04:29:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 12-Jul-2019 04:29:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:29:04

Channel configuration set to: GFP: 12-Jul-2019 04:29:05

Channel:GFP set at:12-Jul-2019 04:29:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 12-Jul-2019 04:29:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:29:05

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:29:07

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 12-Jul-2019 04:29:09

Channel:Brightfield set at:12-Jul-2019 04:29:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 04:29:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:29:09

Channel configuration set to: GFP: 12-Jul-2019 04:29:10

Channel:GFP set at:12-Jul-2019 04:29:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 04:29:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:29:11

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:145 completed at:12-Jul-2019 04:29:13

Time since start of timelapse:43299.8375

PFS is locked

Time to next time point:200.8758



------Time point_146------

Memory available for all arrays: 61057077248

Memory used by Matlab: 2220158976

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:32:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.9

Channel configuration set to: Brightfield: 12-Jul-2019 04:32:35

Channel:Brightfield set at:12-Jul-2019 04:32:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:32:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:32:36

Channel configuration set to: GFP: 12-Jul-2019 04:32:37

Channel:GFP set at:12-Jul-2019 04:32:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:32:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:32:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.975

Channel configuration set to: Brightfield: 12-Jul-2019 04:32:41

Channel:Brightfield set at:12-Jul-2019 04:32:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8751. 12-Jul-2019 04:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:32:42

Channel configuration set to: GFP: 12-Jul-2019 04:32:43

Channel:GFP set at:12-Jul-2019 04:32:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 12-Jul-2019 04:32:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:32:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:32:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 12-Jul-2019 04:32:46

Channel:Brightfield set at:12-Jul-2019 04:32:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 12-Jul-2019 04:32:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:32:46

Channel configuration set to: GFP: 12-Jul-2019 04:32:47

Channel:GFP set at:12-Jul-2019 04:32:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 12-Jul-2019 04:32:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:32:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:32:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.975

Channel configuration set to: Brightfield: 12-Jul-2019 04:32:51

Channel:Brightfield set at:12-Jul-2019 04:32:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 04:32:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:32:51

Channel configuration set to: GFP: 12-Jul-2019 04:32:53

Channel:GFP set at:12-Jul-2019 04:32:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 04:32:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:32:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:32:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.125

Channel configuration set to: Brightfield: 12-Jul-2019 04:32:56

Channel:Brightfield set at:12-Jul-2019 04:32:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 04:32:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:32:57

Channel configuration set to: GFP: 12-Jul-2019 04:32:58

Channel:GFP set at:12-Jul-2019 04:32:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 04:32:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:32:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:02

Channel:Brightfield set at:12-Jul-2019 04:33:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 04:33:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:02

Channel configuration set to: GFP: 12-Jul-2019 04:33:03

Channel:GFP set at:12-Jul-2019 04:33:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 04:33:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:07

Channel:Brightfield set at:12-Jul-2019 04:33:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 04:33:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:08

Channel configuration set to: GFP: 12-Jul-2019 04:33:09

Channel:GFP set at:12-Jul-2019 04:33:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 04:33:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:13

Channel:Brightfield set at:12-Jul-2019 04:33:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 04:33:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:13

Channel configuration set to: GFP: 12-Jul-2019 04:33:14

Channel:GFP set at:12-Jul-2019 04:33:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 04:33:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.025

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:18

Channel:Brightfield set at:12-Jul-2019 04:33:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 04:33:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:19

Channel configuration set to: GFP: 12-Jul-2019 04:33:20

Channel:GFP set at:12-Jul-2019 04:33:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 04:33:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:24

Channel:Brightfield set at:12-Jul-2019 04:33:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 04:33:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:24

Channel configuration set to: GFP: 12-Jul-2019 04:33:25

Channel:GFP set at:12-Jul-2019 04:33:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 04:33:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.225

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:29

Channel:Brightfield set at:12-Jul-2019 04:33:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 04:33:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:29

Channel configuration set to: GFP: 12-Jul-2019 04:33:30

Channel:GFP set at:12-Jul-2019 04:33:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 04:33:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:34

Channel:Brightfield set at:12-Jul-2019 04:33:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 04:33:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:34

Channel configuration set to: GFP: 12-Jul-2019 04:33:36

Channel:GFP set at:12-Jul-2019 04:33:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 04:33:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:39

Channel:Brightfield set at:12-Jul-2019 04:33:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 04:33:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:40

Channel configuration set to: GFP: 12-Jul-2019 04:33:41

Channel:GFP set at:12-Jul-2019 04:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 04:33:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:45

Channel:Brightfield set at:12-Jul-2019 04:33:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 04:33:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:45

Channel configuration set to: GFP: 12-Jul-2019 04:33:47

Channel:GFP set at:12-Jul-2019 04:33:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:33:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:50

Channel:Brightfield set at:12-Jul-2019 04:33:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 04:33:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:50

Channel configuration set to: GFP: 12-Jul-2019 04:33:51

Channel:GFP set at:12-Jul-2019 04:33:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 04:33:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:55

Channel:Brightfield set at:12-Jul-2019 04:33:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 04:33:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:56

Channel configuration set to: GFP: 12-Jul-2019 04:33:57

Channel:GFP set at:12-Jul-2019 04:33:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 04:33:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:33:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:33:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.325

Channel configuration set to: Brightfield: 12-Jul-2019 04:34:00

Channel:Brightfield set at:12-Jul-2019 04:34:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 04:34:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:34:01

Channel configuration set to: GFP: 12-Jul-2019 04:34:02

Channel:GFP set at:12-Jul-2019 04:34:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4501. 12-Jul-2019 04:34:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:34:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:34:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 12-Jul-2019 04:34:06

Channel:Brightfield set at:12-Jul-2019 04:34:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 12-Jul-2019 04:34:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:34:06

Channel configuration set to: GFP: 12-Jul-2019 04:34:07

Channel:GFP set at:12-Jul-2019 04:34:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 04:34:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:34:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:146 completed at:12-Jul-2019 04:34:10

Time since start of timelapse:43596.9189

PFS is locked

Time to next time point:203.7958



------Time point_147------

Memory available for all arrays: 61050175488

Memory used by Matlab: 2225147904

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:37:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-11.85

Channel configuration set to: Brightfield: 12-Jul-2019 04:37:35

Channel:Brightfield set at:12-Jul-2019 04:37:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 04:37:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:37:35

Channel configuration set to: GFP: 12-Jul-2019 04:37:36

Channel:GFP set at:12-Jul-2019 04:37:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7251. 12-Jul-2019 04:37:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:37:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:37:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.925

Channel configuration set to: Brightfield: 12-Jul-2019 04:37:41

Channel:Brightfield set at:12-Jul-2019 04:37:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 12-Jul-2019 04:37:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:37:41

Channel configuration set to: GFP: 12-Jul-2019 04:37:42

Channel:GFP set at:12-Jul-2019 04:37:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 12-Jul-2019 04:37:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:37:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:37:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 12-Jul-2019 04:37:45

Channel:Brightfield set at:12-Jul-2019 04:37:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6501. 12-Jul-2019 04:37:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:37:46

Channel configuration set to: GFP: 12-Jul-2019 04:37:47

Channel:GFP set at:12-Jul-2019 04:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6501. 12-Jul-2019 04:37:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:37:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:37:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.85

Channel configuration set to: Brightfield: 12-Jul-2019 04:37:51

Channel:Brightfield set at:12-Jul-2019 04:37:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 04:37:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:37:51

Channel configuration set to: GFP: 12-Jul-2019 04:37:52

Channel:GFP set at:12-Jul-2019 04:37:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 12-Jul-2019 04:37:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:37:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:37:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.95

Channel configuration set to: Brightfield: 12-Jul-2019 04:37:56

Channel:Brightfield set at:12-Jul-2019 04:37:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 04:37:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:37:56

Channel configuration set to: GFP: 12-Jul-2019 04:37:58

Channel:GFP set at:12-Jul-2019 04:37:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 04:37:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:37:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.225

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:01

Channel:Brightfield set at:12-Jul-2019 04:38:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 04:38:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:02

Channel configuration set to: GFP: 12-Jul-2019 04:38:03

Channel:GFP set at:12-Jul-2019 04:38:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 04:38:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:06

Channel:Brightfield set at:12-Jul-2019 04:38:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 04:38:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:07

Channel configuration set to: GFP: 12-Jul-2019 04:38:08

Channel:GFP set at:12-Jul-2019 04:38:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 04:38:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:08

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:10

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.625

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:12

Channel:Brightfield set at:12-Jul-2019 04:38:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 12-Jul-2019 04:38:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:12

Channel configuration set to: GFP: 12-Jul-2019 04:38:14

Channel:GFP set at:12-Jul-2019 04:38:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4001. 12-Jul-2019 04:38:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:17

Channel:Brightfield set at:12-Jul-2019 04:38:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 04:38:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:18

Channel configuration set to: GFP: 12-Jul-2019 04:38:19

Channel:GFP set at:12-Jul-2019 04:38:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:38:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.55

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:22

Channel:Brightfield set at:12-Jul-2019 04:38:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 04:38:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:23

Channel configuration set to: GFP: 12-Jul-2019 04:38:24

Channel:GFP set at:12-Jul-2019 04:38:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 04:38:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:24

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:28

Channel:Brightfield set at:12-Jul-2019 04:38:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 04:38:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:28

Channel configuration set to: GFP: 12-Jul-2019 04:38:29

Channel:GFP set at:12-Jul-2019 04:38:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 04:38:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:33

Channel:Brightfield set at:12-Jul-2019 04:38:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 04:38:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:33

Channel configuration set to: GFP: 12-Jul-2019 04:38:35

Channel:GFP set at:12-Jul-2019 04:38:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 04:38:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:37

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.725

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:38

Channel:Brightfield set at:12-Jul-2019 04:38:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 04:38:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:39

Channel configuration set to: GFP: 12-Jul-2019 04:38:40

Channel:GFP set at:12-Jul-2019 04:38:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 04:38:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:40

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:44

Channel:Brightfield set at:12-Jul-2019 04:38:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 04:38:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:44

Channel configuration set to: GFP: 12-Jul-2019 04:38:46

Channel:GFP set at:12-Jul-2019 04:38:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 04:38:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:49

Channel:Brightfield set at:12-Jul-2019 04:38:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 04:38:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:49

Channel configuration set to: GFP: 12-Jul-2019 04:38:50

Channel:GFP set at:12-Jul-2019 04:38:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 04:38:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.675

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:54

Channel:Brightfield set at:12-Jul-2019 04:38:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 04:38:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:55

Channel configuration set to: GFP: 12-Jul-2019 04:38:56

Channel:GFP set at:12-Jul-2019 04:38:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7751. 12-Jul-2019 04:38:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:38:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:58

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.15

Channel configuration set to: Brightfield: 12-Jul-2019 04:38:59

Channel:Brightfield set at:12-Jul-2019 04:38:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 04:39:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:39:00

Channel configuration set to: GFP: 12-Jul-2019 04:39:01

Channel:GFP set at:12-Jul-2019 04:39:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 04:39:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:39:01

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:39:03

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.275

Channel configuration set to: Brightfield: 12-Jul-2019 04:39:04

Channel:Brightfield set at:12-Jul-2019 04:39:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0751. 12-Jul-2019 04:39:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:39:05

Channel configuration set to: GFP: 12-Jul-2019 04:39:06

Channel:GFP set at:12-Jul-2019 04:39:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0501. 12-Jul-2019 04:39:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:39:06

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:147 completed at:12-Jul-2019 04:39:08

Time since start of timelapse:43895.5657

PFS is locked

Time to next time point:205.1502



------Time point_148------

Memory available for all arrays: 61042184192

Memory used by Matlab: 2229878784

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:42:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.725

Channel configuration set to: Brightfield: 12-Jul-2019 04:42:35

Channel:Brightfield set at:12-Jul-2019 04:42:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 04:42:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:42:36

Channel configuration set to: GFP: 12-Jul-2019 04:42:37

Channel:GFP set at:12-Jul-2019 04:42:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 04:42:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:42:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:42:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.7

Channel configuration set to: Brightfield: 12-Jul-2019 04:42:41

Channel:Brightfield set at:12-Jul-2019 04:42:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 12-Jul-2019 04:42:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:42:41

Channel configuration set to: GFP: 12-Jul-2019 04:42:42

Channel:GFP set at:12-Jul-2019 04:42:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 12-Jul-2019 04:42:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:42:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:42:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.8

Channel configuration set to: Brightfield: 12-Jul-2019 04:42:46

Channel:Brightfield set at:12-Jul-2019 04:42:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 12-Jul-2019 04:42:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:42:47

Channel configuration set to: GFP: 12-Jul-2019 04:42:48

Channel:GFP set at:12-Jul-2019 04:42:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1501. 12-Jul-2019 04:42:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:42:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:42:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.825

Channel configuration set to: Brightfield: 12-Jul-2019 04:42:51

Channel:Brightfield set at:12-Jul-2019 04:42:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 04:42:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:42:52

Channel configuration set to: GFP: 12-Jul-2019 04:42:53

Channel:GFP set at:12-Jul-2019 04:42:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 12-Jul-2019 04:42:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:42:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:42:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.875

Channel configuration set to: Brightfield: 12-Jul-2019 04:42:57

Channel:Brightfield set at:12-Jul-2019 04:42:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 12-Jul-2019 04:42:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:42:57

Channel configuration set to: GFP: 12-Jul-2019 04:42:58

Channel:GFP set at:12-Jul-2019 04:42:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 04:42:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:42:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:02

Channel:Brightfield set at:12-Jul-2019 04:43:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 04:43:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:03

Channel configuration set to: GFP: 12-Jul-2019 04:43:04

Channel:GFP set at:12-Jul-2019 04:43:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 04:43:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:08

Channel:Brightfield set at:12-Jul-2019 04:43:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 04:43:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:08

Channel configuration set to: GFP: 12-Jul-2019 04:43:10

Channel:GFP set at:12-Jul-2019 04:43:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 04:43:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:13

Channel:Brightfield set at:12-Jul-2019 04:43:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 04:43:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:13

Channel configuration set to: GFP: 12-Jul-2019 04:43:14

Channel:GFP set at:12-Jul-2019 04:43:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 04:43:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:19

Channel:Brightfield set at:12-Jul-2019 04:43:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:43:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:19

Channel configuration set to: GFP: 12-Jul-2019 04:43:20

Channel:GFP set at:12-Jul-2019 04:43:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:43:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:23

Channel:Brightfield set at:12-Jul-2019 04:43:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 12-Jul-2019 04:43:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:24

Channel configuration set to: GFP: 12-Jul-2019 04:43:25

Channel:GFP set at:12-Jul-2019 04:43:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 12-Jul-2019 04:43:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:29

Channel:Brightfield set at:12-Jul-2019 04:43:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 04:43:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:29

Channel configuration set to: GFP: 12-Jul-2019 04:43:30

Channel:GFP set at:12-Jul-2019 04:43:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 04:43:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:35

Channel:Brightfield set at:12-Jul-2019 04:43:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 04:43:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:35

Channel configuration set to: GFP: 12-Jul-2019 04:43:36

Channel:GFP set at:12-Jul-2019 04:43:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 04:43:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:39

Channel:Brightfield set at:12-Jul-2019 04:43:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 12-Jul-2019 04:43:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:40

Channel configuration set to: GFP: 12-Jul-2019 04:43:41

Channel:GFP set at:12-Jul-2019 04:43:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 12-Jul-2019 04:43:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:45

Channel:Brightfield set at:12-Jul-2019 04:43:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 04:43:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:45

Channel configuration set to: GFP: 12-Jul-2019 04:43:47

Channel:GFP set at:12-Jul-2019 04:43:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 04:43:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:50

Channel:Brightfield set at:12-Jul-2019 04:43:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 04:43:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:50

Channel configuration set to: GFP: 12-Jul-2019 04:43:51

Channel:GFP set at:12-Jul-2019 04:43:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 04:43:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.325

Channel configuration set to: Brightfield: 12-Jul-2019 04:43:56

Channel:Brightfield set at:12-Jul-2019 04:43:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 04:43:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:56

Channel configuration set to: GFP: 12-Jul-2019 04:43:57

Channel:GFP set at:12-Jul-2019 04:43:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 04:43:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:43:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:44:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 12-Jul-2019 04:44:00

Channel:Brightfield set at:12-Jul-2019 04:44:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 12-Jul-2019 04:44:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:44:01

Channel configuration set to: GFP: 12-Jul-2019 04:44:02

Channel:GFP set at:12-Jul-2019 04:44:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 12-Jul-2019 04:44:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:44:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:44:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 04:44:06

Channel:Brightfield set at:12-Jul-2019 04:44:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 04:44:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:44:06

Channel configuration set to: GFP: 12-Jul-2019 04:44:08

Channel:GFP set at:12-Jul-2019 04:44:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 04:44:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:44:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:148 completed at:12-Jul-2019 04:44:10

Time since start of timelapse:44197.2608

PFS is locked

Time to next time point:203.4563



------Time point_149------

Memory available for all arrays: 61036953600

Memory used by Matlab: 2234081280

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:47:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.6

Channel configuration set to: Brightfield: 12-Jul-2019 04:47:35

Channel:Brightfield set at:12-Jul-2019 04:47:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 04:47:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:47:36

Channel configuration set to: GFP: 12-Jul-2019 04:47:37

Channel:GFP set at:12-Jul-2019 04:47:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 04:47:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:47:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:47:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.6

Channel configuration set to: Brightfield: 12-Jul-2019 04:47:41

Channel:Brightfield set at:12-Jul-2019 04:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 12-Jul-2019 04:47:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:47:41

Channel configuration set to: GFP: 12-Jul-2019 04:47:42

Channel:GFP set at:12-Jul-2019 04:47:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 12-Jul-2019 04:47:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:47:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:47:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 12-Jul-2019 04:47:46

Channel:Brightfield set at:12-Jul-2019 04:47:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9001. 12-Jul-2019 04:47:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:47:46

Channel configuration set to: GFP: 12-Jul-2019 04:47:47

Channel:GFP set at:12-Jul-2019 04:47:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.9001. 12-Jul-2019 04:47:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:47:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:47:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.825

Channel configuration set to: Brightfield: 12-Jul-2019 04:47:51

Channel:Brightfield set at:12-Jul-2019 04:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 12-Jul-2019 04:47:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:47:52

Channel configuration set to: GFP: 12-Jul-2019 04:47:53

Channel:GFP set at:12-Jul-2019 04:47:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8251. 12-Jul-2019 04:47:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:47:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:47:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.275

Channel configuration set to: Brightfield: 12-Jul-2019 04:47:56

Channel:Brightfield set at:12-Jul-2019 04:47:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 12-Jul-2019 04:47:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:47:57

Channel configuration set to: GFP: 12-Jul-2019 04:47:58

Channel:GFP set at:12-Jul-2019 04:47:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 12-Jul-2019 04:47:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:47:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:02

Channel:Brightfield set at:12-Jul-2019 04:48:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 12-Jul-2019 04:48:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:02

Channel configuration set to: GFP: 12-Jul-2019 04:48:03

Channel:GFP set at:12-Jul-2019 04:48:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 12-Jul-2019 04:48:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:07

Channel:Brightfield set at:12-Jul-2019 04:48:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 04:48:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:07

Channel configuration set to: GFP: 12-Jul-2019 04:48:09

Channel:GFP set at:12-Jul-2019 04:48:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 04:48:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:13

Channel:Brightfield set at:12-Jul-2019 04:48:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 04:48:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:14

Channel configuration set to: GFP: 12-Jul-2019 04:48:15

Channel:GFP set at:12-Jul-2019 04:48:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 04:48:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.775

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:19

Channel:Brightfield set at:12-Jul-2019 04:48:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 04:48:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:19

Channel configuration set to: GFP: 12-Jul-2019 04:48:21

Channel:GFP set at:12-Jul-2019 04:48:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 04:48:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.35

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:24

Channel:Brightfield set at:12-Jul-2019 04:48:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6251. 12-Jul-2019 04:48:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:24

Channel configuration set to: GFP: 12-Jul-2019 04:48:25

Channel:GFP set at:12-Jul-2019 04:48:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 12-Jul-2019 04:48:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.175

PFS not locked. Waiting for lock...

PFS took 0.250000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:29

Channel:Brightfield set at:12-Jul-2019 04:48:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 04:48:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:29

Channel configuration set to: GFP: 12-Jul-2019 04:48:31

Channel:GFP set at:12-Jul-2019 04:48:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 04:48:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:34

Channel:Brightfield set at:12-Jul-2019 04:48:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 04:48:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:35

Channel configuration set to: GFP: 12-Jul-2019 04:48:36

Channel:GFP set at:12-Jul-2019 04:48:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 04:48:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.05

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:40

Channel:Brightfield set at:12-Jul-2019 04:48:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:48:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:40

Channel configuration set to: GFP: 12-Jul-2019 04:48:42

Channel:GFP set at:12-Jul-2019 04:48:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:48:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:46

Channel:Brightfield set at:12-Jul-2019 04:48:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 04:48:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:46

Channel configuration set to: GFP: 12-Jul-2019 04:48:47

Channel:GFP set at:12-Jul-2019 04:48:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 04:48:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:51

Channel:Brightfield set at:12-Jul-2019 04:48:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 04:48:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:51

Channel configuration set to: GFP: 12-Jul-2019 04:48:52

Channel:GFP set at:12-Jul-2019 04:48:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 04:48:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.325

Channel configuration set to: Brightfield: 12-Jul-2019 04:48:56

Channel:Brightfield set at:12-Jul-2019 04:48:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 12-Jul-2019 04:48:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:57

Channel configuration set to: GFP: 12-Jul-2019 04:48:58

Channel:GFP set at:12-Jul-2019 04:48:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0501. 12-Jul-2019 04:48:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:48:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:49:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.6

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 04:49:02

Channel:Brightfield set at:12-Jul-2019 04:49:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 12-Jul-2019 04:49:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:49:02

Channel configuration set to: GFP: 12-Jul-2019 04:49:03

Channel:GFP set at:12-Jul-2019 04:49:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 12-Jul-2019 04:49:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:49:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:49:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 04:49:07

Channel:Brightfield set at:12-Jul-2019 04:49:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 12-Jul-2019 04:49:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:49:07

Channel configuration set to: GFP: 12-Jul-2019 04:49:09

Channel:GFP set at:12-Jul-2019 04:49:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 12-Jul-2019 04:49:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:49:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:149 completed at:12-Jul-2019 04:49:11

Time since start of timelapse:44498.6242

PFS is locked

Time to next time point:202.0946



------Time point_150------

Memory available for all arrays: 61035167744

Memory used by Matlab: 2237730816

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:52:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.7

Channel configuration set to: Brightfield: 12-Jul-2019 04:52:35

Channel:Brightfield set at:12-Jul-2019 04:52:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 04:52:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:52:36

Channel configuration set to: GFP: 12-Jul-2019 04:52:37

Channel:GFP set at:12-Jul-2019 04:52:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 04:52:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:52:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:52:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.7

Channel configuration set to: Brightfield: 12-Jul-2019 04:52:41

Channel:Brightfield set at:12-Jul-2019 04:52:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 12-Jul-2019 04:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:52:41

Channel configuration set to: GFP: 12-Jul-2019 04:52:42

Channel:GFP set at:12-Jul-2019 04:52:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1751. 12-Jul-2019 04:52:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:52:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:52:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.725

Channel configuration set to: Brightfield: 12-Jul-2019 04:52:46

Channel:Brightfield set at:12-Jul-2019 04:52:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 12-Jul-2019 04:52:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:52:47

Channel configuration set to: GFP: 12-Jul-2019 04:52:48

Channel:GFP set at:12-Jul-2019 04:52:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 12-Jul-2019 04:52:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:52:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:52:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.175

Channel configuration set to: Brightfield: 12-Jul-2019 04:52:52

Channel:Brightfield set at:12-Jul-2019 04:52:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 04:52:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:52:52

Channel configuration set to: GFP: 12-Jul-2019 04:52:54

Channel:GFP set at:12-Jul-2019 04:52:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 04:52:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:52:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:52:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.85

Channel configuration set to: Brightfield: 12-Jul-2019 04:52:58

Channel:Brightfield set at:12-Jul-2019 04:52:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 04:52:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:52:58

Channel configuration set to: GFP: 12-Jul-2019 04:52:59

Channel:GFP set at:12-Jul-2019 04:52:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 04:53:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:00

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:02

Channel:Brightfield set at:12-Jul-2019 04:53:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 04:53:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:03

Channel configuration set to: GFP: 12-Jul-2019 04:53:04

Channel:GFP set at:12-Jul-2019 04:53:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 04:53:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:09

Channel:Brightfield set at:12-Jul-2019 04:53:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 04:53:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:09

Channel configuration set to: GFP: 12-Jul-2019 04:53:10

Channel:GFP set at:12-Jul-2019 04:53:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 04:53:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:11

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:13

Channel:Brightfield set at:12-Jul-2019 04:53:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 04:53:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:14

Channel configuration set to: GFP: 12-Jul-2019 04:53:15

Channel:GFP set at:12-Jul-2019 04:53:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 04:53:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:18

Channel:Brightfield set at:12-Jul-2019 04:53:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 04:53:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:19

Channel configuration set to: GFP: 12-Jul-2019 04:53:20

Channel:GFP set at:12-Jul-2019 04:53:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 04:53:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.5

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:24

Channel:Brightfield set at:12-Jul-2019 04:53:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 04:53:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:24

Channel configuration set to: GFP: 12-Jul-2019 04:53:26

Channel:GFP set at:12-Jul-2019 04:53:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 04:53:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:29

Channel:Brightfield set at:12-Jul-2019 04:53:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 04:53:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:30

Channel configuration set to: GFP: 12-Jul-2019 04:53:31

Channel:GFP set at:12-Jul-2019 04:53:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 04:53:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.2

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:34

Channel:Brightfield set at:12-Jul-2019 04:53:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 04:53:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:35

Channel configuration set to: GFP: 12-Jul-2019 04:53:36

Channel:GFP set at:12-Jul-2019 04:53:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 04:53:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:40

Channel:Brightfield set at:12-Jul-2019 04:53:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 04:53:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:40

Channel configuration set to: GFP: 12-Jul-2019 04:53:41

Channel:GFP set at:12-Jul-2019 04:53:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.1251. 12-Jul-2019 04:53:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:45

Channel:Brightfield set at:12-Jul-2019 04:53:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:53:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:45

Channel configuration set to: GFP: 12-Jul-2019 04:53:47

Channel:GFP set at:12-Jul-2019 04:53:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:53:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:50

Channel:Brightfield set at:12-Jul-2019 04:53:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 04:53:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:50

Channel configuration set to: GFP: 12-Jul-2019 04:53:52

Channel:GFP set at:12-Jul-2019 04:53:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 04:53:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.475

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:55

Channel:Brightfield set at:12-Jul-2019 04:53:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9251. 12-Jul-2019 04:53:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:56

Channel configuration set to: GFP: 12-Jul-2019 04:53:57

Channel:GFP set at:12-Jul-2019 04:53:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9501. 12-Jul-2019 04:53:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:53:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:53:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5

Channel configuration set to: Brightfield: 12-Jul-2019 04:54:00

Channel:Brightfield set at:12-Jul-2019 04:54:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 12-Jul-2019 04:54:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:54:01

Channel configuration set to: GFP: 12-Jul-2019 04:54:02

Channel:GFP set at:12-Jul-2019 04:54:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7751. 12-Jul-2019 04:54:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:54:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:54:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 04:54:05

Channel:Brightfield set at:12-Jul-2019 04:54:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2751. 12-Jul-2019 04:54:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:54:06

Channel configuration set to: GFP: 12-Jul-2019 04:54:07

Channel:GFP set at:12-Jul-2019 04:54:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 12-Jul-2019 04:54:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:54:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:150 completed at:12-Jul-2019 04:54:10

Time since start of timelapse:44796.9501

PFS is locked

Time to next time point:203.77



------Time point_151------

Memory available for all arrays: 61030641664

Memory used by Matlab: 2241564672

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:57:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.675

Channel configuration set to: Brightfield: 12-Jul-2019 04:57:35

Channel:Brightfield set at:12-Jul-2019 04:57:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 04:57:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:57:36

Channel configuration set to: GFP: 12-Jul-2019 04:57:37

Channel:GFP set at:12-Jul-2019 04:57:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 04:57:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:57:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:57:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.775

Channel configuration set to: Brightfield: 12-Jul-2019 04:57:41

Channel:Brightfield set at:12-Jul-2019 04:57:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 04:57:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:57:41

Channel configuration set to: GFP: 12-Jul-2019 04:57:42

Channel:GFP set at:12-Jul-2019 04:57:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 04:57:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:57:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:57:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 12-Jul-2019 04:57:45

Channel:Brightfield set at:12-Jul-2019 04:57:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 12-Jul-2019 04:57:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:57:46

Channel configuration set to: GFP: 12-Jul-2019 04:57:47

Channel:GFP set at:12-Jul-2019 04:57:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7001. 12-Jul-2019 04:57:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:57:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:57:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.875

Channel configuration set to: Brightfield: 12-Jul-2019 04:57:51

Channel:Brightfield set at:12-Jul-2019 04:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 04:57:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:57:51

Channel configuration set to: GFP: 12-Jul-2019 04:57:53

Channel:GFP set at:12-Jul-2019 04:57:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 04:57:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:57:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:57:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.975

Channel configuration set to: Brightfield: 12-Jul-2019 04:57:56

Channel:Brightfield set at:12-Jul-2019 04:57:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 04:57:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:57:57

Channel configuration set to: GFP: 12-Jul-2019 04:57:58

Channel:GFP set at:12-Jul-2019 04:57:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 04:57:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:57:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:02

Channel:Brightfield set at:12-Jul-2019 04:58:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 04:58:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:02

Channel configuration set to: GFP: 12-Jul-2019 04:58:03

Channel:GFP set at:12-Jul-2019 04:58:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 04:58:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:08

Channel:Brightfield set at:12-Jul-2019 04:58:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 04:58:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:09

Channel configuration set to: GFP: 12-Jul-2019 04:58:10

Channel:GFP set at:12-Jul-2019 04:58:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 04:58:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.65

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:14

Channel:Brightfield set at:12-Jul-2019 04:58:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3501. 12-Jul-2019 04:58:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:14

Channel configuration set to: GFP: 12-Jul-2019 04:58:15

Channel:GFP set at:12-Jul-2019 04:58:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3501. 12-Jul-2019 04:58:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:19

Channel:Brightfield set at:12-Jul-2019 04:58:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 04:58:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:20

Channel configuration set to: GFP: 12-Jul-2019 04:58:21

Channel:GFP set at:12-Jul-2019 04:58:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 04:58:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:24

Channel:Brightfield set at:12-Jul-2019 04:58:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 04:58:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:24

Channel configuration set to: GFP: 12-Jul-2019 04:58:26

Channel:GFP set at:12-Jul-2019 04:58:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 04:58:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:29

Channel:Brightfield set at:12-Jul-2019 04:58:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 04:58:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:30

Channel configuration set to: GFP: 12-Jul-2019 04:58:31

Channel:GFP set at:12-Jul-2019 04:58:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 04:58:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:35

Channel:Brightfield set at:12-Jul-2019 04:58:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 04:58:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:35

Channel configuration set to: GFP: 12-Jul-2019 04:58:36

Channel:GFP set at:12-Jul-2019 04:58:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 04:58:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.575

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:40

Channel:Brightfield set at:12-Jul-2019 04:58:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 04:58:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:40

Channel configuration set to: GFP: 12-Jul-2019 04:58:42

Channel:GFP set at:12-Jul-2019 04:58:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 04:58:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:46

Channel:Brightfield set at:12-Jul-2019 04:58:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 04:58:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:46

Channel configuration set to: GFP: 12-Jul-2019 04:58:48

Channel:GFP set at:12-Jul-2019 04:58:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 04:58:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.85

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:51

Channel:Brightfield set at:12-Jul-2019 04:58:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:58:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:51

Channel configuration set to: GFP: 12-Jul-2019 04:58:52

Channel:GFP set at:12-Jul-2019 04:58:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 04:58:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 12-Jul-2019 04:58:56

Channel:Brightfield set at:12-Jul-2019 04:58:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7751. 12-Jul-2019 04:58:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:56

Channel configuration set to: GFP: 12-Jul-2019 04:58:58

Channel:GFP set at:12-Jul-2019 04:58:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 04:58:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:58:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:59:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 12-Jul-2019 04:59:01

Channel:Brightfield set at:12-Jul-2019 04:59:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 04:59:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:59:01

Channel configuration set to: GFP: 12-Jul-2019 04:59:02

Channel:GFP set at:12-Jul-2019 04:59:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 04:59:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:59:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 04:59:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-4.65

Channel configuration set to: Brightfield: 12-Jul-2019 04:59:06

Channel:Brightfield set at:12-Jul-2019 04:59:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 12-Jul-2019 04:59:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:59:06

Channel configuration set to: GFP: 12-Jul-2019 04:59:07

Channel:GFP set at:12-Jul-2019 04:59:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 12-Jul-2019 04:59:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 04:59:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:151 completed at:12-Jul-2019 04:59:10

Time since start of timelapse:45097.0534

PFS is locked

Time to next time point:203.6682



------Time point_152------

Memory available for all arrays: 61025501184

Memory used by Matlab: 2246606848

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:02:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.75

Channel configuration set to: Brightfield: 12-Jul-2019 05:02:35

Channel:Brightfield set at:12-Jul-2019 05:02:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 05:02:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:02:36

Channel configuration set to: GFP: 12-Jul-2019 05:02:37

Channel:GFP set at:12-Jul-2019 05:02:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 05:02:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:02:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:02:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-8.9

Channel configuration set to: Brightfield: 12-Jul-2019 05:02:41

Channel:Brightfield set at:12-Jul-2019 05:02:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 05:02:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:02:41

Channel configuration set to: GFP: 12-Jul-2019 05:02:43

Channel:GFP set at:12-Jul-2019 05:02:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0001. 12-Jul-2019 05:02:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:02:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:02:46

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 12-Jul-2019 05:02:47

Channel:Brightfield set at:12-Jul-2019 05:02:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 12-Jul-2019 05:02:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:02:47

Channel configuration set to: GFP: 12-Jul-2019 05:02:48

Channel:GFP set at:12-Jul-2019 05:02:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.7251. 12-Jul-2019 05:02:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:02:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:02:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.025

Channel configuration set to: Brightfield: 12-Jul-2019 05:02:52

Channel:Brightfield set at:12-Jul-2019 05:02:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 05:02:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:02:52

Channel configuration set to: GFP: 12-Jul-2019 05:02:54

Channel:GFP set at:12-Jul-2019 05:02:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 05:02:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:02:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:02:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9

Channel configuration set to: Brightfield: 12-Jul-2019 05:02:57

Channel:Brightfield set at:12-Jul-2019 05:02:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 05:02:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:02:58

Channel configuration set to: GFP: 12-Jul-2019 05:02:59

Channel:GFP set at:12-Jul-2019 05:02:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 05:03:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:00

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:02

Channel:Brightfield set at:12-Jul-2019 05:03:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2001. 12-Jul-2019 05:03:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:03

Channel configuration set to: GFP: 12-Jul-2019 05:03:04

Channel:GFP set at:12-Jul-2019 05:03:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2001. 12-Jul-2019 05:03:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:07

Channel:Brightfield set at:12-Jul-2019 05:03:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 05:03:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:08

Channel configuration set to: GFP: 12-Jul-2019 05:03:09

Channel:GFP set at:12-Jul-2019 05:03:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 05:03:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.625

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:13

Channel:Brightfield set at:12-Jul-2019 05:03:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3251. 12-Jul-2019 05:03:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:14

Channel configuration set to: GFP: 12-Jul-2019 05:03:15

Channel:GFP set at:12-Jul-2019 05:03:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3501. 12-Jul-2019 05:03:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:19

Channel:Brightfield set at:12-Jul-2019 05:03:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 05:03:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:19

Channel configuration set to: GFP: 12-Jul-2019 05:03:20

Channel:GFP set at:12-Jul-2019 05:03:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 05:03:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:24

Channel:Brightfield set at:12-Jul-2019 05:03:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 05:03:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:25

Channel configuration set to: GFP: 12-Jul-2019 05:03:26

Channel:GFP set at:12-Jul-2019 05:03:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 05:03:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:30

Channel:Brightfield set at:12-Jul-2019 05:03:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0001. 12-Jul-2019 05:03:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:30

Channel configuration set to: GFP: 12-Jul-2019 05:03:31

Channel:GFP set at:12-Jul-2019 05:03:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0001. 12-Jul-2019 05:03:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:35

Channel:Brightfield set at:12-Jul-2019 05:03:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 05:03:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:35

Channel configuration set to: GFP: 12-Jul-2019 05:03:36

Channel:GFP set at:12-Jul-2019 05:03:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 05:03:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:41

Channel:Brightfield set at:12-Jul-2019 05:03:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 05:03:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:41

Channel configuration set to: GFP: 12-Jul-2019 05:03:42

Channel:GFP set at:12-Jul-2019 05:03:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 05:03:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:46

Channel:Brightfield set at:12-Jul-2019 05:03:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 05:03:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:47

Channel configuration set to: GFP: 12-Jul-2019 05:03:48

Channel:GFP set at:12-Jul-2019 05:03:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 05:03:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:51

Channel:Brightfield set at:12-Jul-2019 05:03:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:03:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:52

Channel configuration set to: GFP: 12-Jul-2019 05:03:53

Channel:GFP set at:12-Jul-2019 05:03:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:03:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 12-Jul-2019 05:03:57

Channel:Brightfield set at:12-Jul-2019 05:03:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 05:03:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:57

Channel configuration set to: GFP: 12-Jul-2019 05:03:59

Channel:GFP set at:12-Jul-2019 05:03:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 05:03:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:03:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:04:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 12-Jul-2019 05:04:02

Channel:Brightfield set at:12-Jul-2019 05:04:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 05:04:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:04:02

Channel configuration set to: GFP: 12-Jul-2019 05:04:04

Channel:GFP set at:12-Jul-2019 05:04:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 05:04:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:04:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:04:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 12-Jul-2019 05:04:07

Channel:Brightfield set at:12-Jul-2019 05:04:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 05:04:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:04:08

Channel configuration set to: GFP: 12-Jul-2019 05:04:09

Channel:GFP set at:12-Jul-2019 05:04:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 05:04:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:04:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:152 completed at:12-Jul-2019 05:04:12

Time since start of timelapse:45398.8461

PFS is locked

Time to next time point:201.8769



------Time point_153------

Memory available for all arrays: 61006090240

Memory used by Matlab: 2249232384

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:07:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.975

Channel configuration set to: Brightfield: 12-Jul-2019 05:07:35

Channel:Brightfield set at:12-Jul-2019 05:07:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:07:36

Channel configuration set to: GFP: 12-Jul-2019 05:07:37

Channel:GFP set at:12-Jul-2019 05:07:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:07:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:07:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:07:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 12-Jul-2019 05:07:41

Channel:Brightfield set at:12-Jul-2019 05:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 05:07:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:07:41

Channel configuration set to: GFP: 12-Jul-2019 05:07:43

Channel:GFP set at:12-Jul-2019 05:07:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 05:07:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:07:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:07:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.175

Channel configuration set to: Brightfield: 12-Jul-2019 05:07:46

Channel:Brightfield set at:12-Jul-2019 05:07:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 12-Jul-2019 05:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:07:46

Channel configuration set to: GFP: 12-Jul-2019 05:07:48

Channel:GFP set at:12-Jul-2019 05:07:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 12-Jul-2019 05:07:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:07:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:07:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.3

Channel configuration set to: Brightfield: 12-Jul-2019 05:07:51

Channel:Brightfield set at:12-Jul-2019 05:07:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:07:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:07:52

Channel configuration set to: GFP: 12-Jul-2019 05:07:53

Channel:GFP set at:12-Jul-2019 05:07:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:07:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:07:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:07:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 05:07:56

Channel:Brightfield set at:12-Jul-2019 05:07:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 05:07:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:07:56

Channel configuration set to: GFP: 12-Jul-2019 05:07:58

Channel:GFP set at:12-Jul-2019 05:07:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 05:07:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:07:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.5

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:01

Channel:Brightfield set at:12-Jul-2019 05:08:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 12-Jul-2019 05:08:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:02

Channel configuration set to: GFP: 12-Jul-2019 05:08:03

Channel:GFP set at:12-Jul-2019 05:08:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 12-Jul-2019 05:08:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:07

Channel:Brightfield set at:12-Jul-2019 05:08:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:08:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:08

Channel configuration set to: GFP: 12-Jul-2019 05:08:09

Channel:GFP set at:12-Jul-2019 05:08:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:08:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:12

Channel:Brightfield set at:12-Jul-2019 05:08:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:08:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:13

Channel configuration set to: GFP: 12-Jul-2019 05:08:14

Channel:GFP set at:12-Jul-2019 05:08:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:08:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:18

Channel:Brightfield set at:12-Jul-2019 05:08:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:08:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:18

Channel configuration set to: GFP: 12-Jul-2019 05:08:20

Channel:GFP set at:12-Jul-2019 05:08:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:08:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:23

Channel:Brightfield set at:12-Jul-2019 05:08:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 05:08:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:24

Channel configuration set to: GFP: 12-Jul-2019 05:08:25

Channel:GFP set at:12-Jul-2019 05:08:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 05:08:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:30

Channel:Brightfield set at:12-Jul-2019 05:08:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 05:08:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:30

Channel configuration set to: GFP: 12-Jul-2019 05:08:31

Channel:GFP set at:12-Jul-2019 05:08:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 05:08:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:35

Channel:Brightfield set at:12-Jul-2019 05:08:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0001. 12-Jul-2019 05:08:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:35

Channel configuration set to: GFP: 12-Jul-2019 05:08:36

Channel:GFP set at:12-Jul-2019 05:08:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0001. 12-Jul-2019 05:08:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:41

Channel:Brightfield set at:12-Jul-2019 05:08:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 05:08:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:41

Channel configuration set to: GFP: 12-Jul-2019 05:08:42

Channel:GFP set at:12-Jul-2019 05:08:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 05:08:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:46

Channel:Brightfield set at:12-Jul-2019 05:08:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 05:08:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:47

Channel configuration set to: GFP: 12-Jul-2019 05:08:48

Channel:GFP set at:12-Jul-2019 05:08:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 05:08:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:51

Channel:Brightfield set at:12-Jul-2019 05:08:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:08:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:51

Channel configuration set to: GFP: 12-Jul-2019 05:08:53

Channel:GFP set at:12-Jul-2019 05:08:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:08:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 12-Jul-2019 05:08:57

Channel:Brightfield set at:12-Jul-2019 05:08:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 05:08:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:57

Channel configuration set to: GFP: 12-Jul-2019 05:08:58

Channel:GFP set at:12-Jul-2019 05:08:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 05:08:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:08:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:09:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.375

Channel configuration set to: Brightfield: 12-Jul-2019 05:09:02

Channel:Brightfield set at:12-Jul-2019 05:09:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 05:09:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:09:02

Channel configuration set to: GFP: 12-Jul-2019 05:09:03

Channel:GFP set at:12-Jul-2019 05:09:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 12-Jul-2019 05:09:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:09:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:09:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 05:09:07

Channel:Brightfield set at:12-Jul-2019 05:09:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 05:09:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:09:08

Channel configuration set to: GFP: 12-Jul-2019 05:09:09

Channel:GFP set at:12-Jul-2019 05:09:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7001. 12-Jul-2019 05:09:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:09:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:153 completed at:12-Jul-2019 05:09:11

Time since start of timelapse:45698.3972

PFS is locked

Time to next time point:202.3272



------Time point_154------

Memory available for all arrays: 61015502848

Memory used by Matlab: 2252386304

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:12:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.025

Channel configuration set to: Brightfield: 12-Jul-2019 05:12:35

Channel:Brightfield set at:12-Jul-2019 05:12:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:12:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:12:36

Channel configuration set to: GFP: 12-Jul-2019 05:12:37

Channel:GFP set at:12-Jul-2019 05:12:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:12:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:12:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:12:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-11.225

Channel configuration set to: Brightfield: 12-Jul-2019 05:12:41

Channel:Brightfield set at:12-Jul-2019 05:12:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 05:12:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:12:41

Channel configuration set to: GFP: 12-Jul-2019 05:12:42

Channel:GFP set at:12-Jul-2019 05:12:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 05:12:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:12:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:12:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 12-Jul-2019 05:12:46

Channel:Brightfield set at:12-Jul-2019 05:12:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 05:12:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:12:46

Channel configuration set to: GFP: 12-Jul-2019 05:12:47

Channel:GFP set at:12-Jul-2019 05:12:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4251. 12-Jul-2019 05:12:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:12:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:12:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.225

Channel configuration set to: Brightfield: 12-Jul-2019 05:12:51

Channel:Brightfield set at:12-Jul-2019 05:12:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 05:12:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:12:51

Channel configuration set to: GFP: 12-Jul-2019 05:12:53

Channel:GFP set at:12-Jul-2019 05:12:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 05:12:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:12:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:12:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 12-Jul-2019 05:12:57

Channel:Brightfield set at:12-Jul-2019 05:12:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6501. 12-Jul-2019 05:12:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:12:57

Channel configuration set to: GFP: 12-Jul-2019 05:12:58

Channel:GFP set at:12-Jul-2019 05:12:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6251. 12-Jul-2019 05:12:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:12:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.525

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:02

Channel:Brightfield set at:12-Jul-2019 05:13:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 05:13:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:03

Channel configuration set to: GFP: 12-Jul-2019 05:13:04

Channel:GFP set at:12-Jul-2019 05:13:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 05:13:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:08

Channel:Brightfield set at:12-Jul-2019 05:13:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:13:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:08

Channel configuration set to: GFP: 12-Jul-2019 05:13:10

Channel:GFP set at:12-Jul-2019 05:13:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:13:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.925

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:13

Channel:Brightfield set at:12-Jul-2019 05:13:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0001. 12-Jul-2019 05:13:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:14

Channel configuration set to: GFP: 12-Jul-2019 05:13:15

Channel:GFP set at:12-Jul-2019 05:13:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 05:13:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:19

Channel:Brightfield set at:12-Jul-2019 05:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 05:13:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:19

Channel configuration set to: GFP: 12-Jul-2019 05:13:21

Channel:GFP set at:12-Jul-2019 05:13:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 05:13:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:24

Channel:Brightfield set at:12-Jul-2019 05:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 05:13:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:25

Channel configuration set to: GFP: 12-Jul-2019 05:13:26

Channel:GFP set at:12-Jul-2019 05:13:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 05:13:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:29

Channel:Brightfield set at:12-Jul-2019 05:13:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 05:13:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:30

Channel configuration set to: GFP: 12-Jul-2019 05:13:31

Channel:GFP set at:12-Jul-2019 05:13:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7501. 12-Jul-2019 05:13:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:35

Channel:Brightfield set at:12-Jul-2019 05:13:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0001. 12-Jul-2019 05:13:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:35

Channel configuration set to: GFP: 12-Jul-2019 05:13:36

Channel:GFP set at:12-Jul-2019 05:13:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0001. 12-Jul-2019 05:13:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:41

Channel:Brightfield set at:12-Jul-2019 05:13:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 05:13:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:41

Channel configuration set to: GFP: 12-Jul-2019 05:13:42

Channel:GFP set at:12-Jul-2019 05:13:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 05:13:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:46

Channel:Brightfield set at:12-Jul-2019 05:13:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 05:13:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:47

Channel configuration set to: GFP: 12-Jul-2019 05:13:48

Channel:GFP set at:12-Jul-2019 05:13:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 05:13:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:51

Channel:Brightfield set at:12-Jul-2019 05:13:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:13:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:51

Channel configuration set to: GFP: 12-Jul-2019 05:13:53

Channel:GFP set at:12-Jul-2019 05:13:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:13:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 12-Jul-2019 05:13:56

Channel:Brightfield set at:12-Jul-2019 05:13:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 05:13:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:57

Channel configuration set to: GFP: 12-Jul-2019 05:13:58

Channel:GFP set at:12-Jul-2019 05:13:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4751. 12-Jul-2019 05:13:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:13:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:14:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 12-Jul-2019 05:14:02

Channel:Brightfield set at:12-Jul-2019 05:14:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 12-Jul-2019 05:14:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:14:02

Channel configuration set to: GFP: 12-Jul-2019 05:14:03

Channel:GFP set at:12-Jul-2019 05:14:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 05:14:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:14:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:14:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.7

Channel configuration set to: Brightfield: 12-Jul-2019 05:14:07

Channel:Brightfield set at:12-Jul-2019 05:14:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 05:14:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:14:08

Channel configuration set to: GFP: 12-Jul-2019 05:14:09

Channel:GFP set at:12-Jul-2019 05:14:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 05:14:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:14:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:154 completed at:12-Jul-2019 05:14:11

Time since start of timelapse:45998.5416

PFS is locked

Time to next time point:202.1845



------Time point_155------

Memory available for all arrays: 61013807104

Memory used by Matlab: 2256064512

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:17:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.05

Channel configuration set to: Brightfield: 12-Jul-2019 05:17:35

Channel:Brightfield set at:12-Jul-2019 05:17:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 05:17:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:17:36

Channel configuration set to: GFP: 12-Jul-2019 05:17:37

Channel:GFP set at:12-Jul-2019 05:17:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 05:17:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:17:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:17:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.45

Channel configuration set to: Brightfield: 12-Jul-2019 05:17:42

Channel:Brightfield set at:12-Jul-2019 05:17:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 05:17:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:17:42

Channel configuration set to: GFP: 12-Jul-2019 05:17:43

Channel:GFP set at:12-Jul-2019 05:17:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 05:17:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:17:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:17:46

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 12-Jul-2019 05:17:47

Channel:Brightfield set at:12-Jul-2019 05:17:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 12-Jul-2019 05:17:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:17:47

Channel configuration set to: GFP: 12-Jul-2019 05:17:48

Channel:GFP set at:12-Jul-2019 05:17:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 12-Jul-2019 05:17:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:17:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:17:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.15

Channel configuration set to: Brightfield: 12-Jul-2019 05:17:52

Channel:Brightfield set at:12-Jul-2019 05:17:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 05:17:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:17:52

Channel configuration set to: GFP: 12-Jul-2019 05:17:54

Channel:GFP set at:12-Jul-2019 05:17:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 05:17:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:17:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:17:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.275

Channel configuration set to: Brightfield: 12-Jul-2019 05:17:58

Channel:Brightfield set at:12-Jul-2019 05:17:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 05:17:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:17:58

Channel configuration set to: GFP: 12-Jul-2019 05:17:59

Channel:GFP set at:12-Jul-2019 05:17:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 05:18:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:00

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.55

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:03

Channel:Brightfield set at:12-Jul-2019 05:18:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 12-Jul-2019 05:18:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:03

Channel configuration set to: GFP: 12-Jul-2019 05:18:04

Channel:GFP set at:12-Jul-2019 05:18:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7001. 12-Jul-2019 05:18:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.875

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:08

Channel:Brightfield set at:12-Jul-2019 05:18:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 05:18:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:08

Channel configuration set to: GFP: 12-Jul-2019 05:18:10

Channel:GFP set at:12-Jul-2019 05:18:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 05:18:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:13

Channel:Brightfield set at:12-Jul-2019 05:18:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 05:18:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:13

Channel configuration set to: GFP: 12-Jul-2019 05:18:15

Channel:GFP set at:12-Jul-2019 05:18:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 05:18:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.25

PFS not locked. Waiting for lock...

PFS took 0.050000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:18

Channel:Brightfield set at:12-Jul-2019 05:18:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 05:18:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:19

Channel configuration set to: GFP: 12-Jul-2019 05:18:20

Channel:GFP set at:12-Jul-2019 05:18:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 05:18:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:24

Channel:Brightfield set at:12-Jul-2019 05:18:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 05:18:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:24

Channel configuration set to: GFP: 12-Jul-2019 05:18:25

Channel:GFP set at:12-Jul-2019 05:18:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 05:18:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.425

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:29

Channel:Brightfield set at:12-Jul-2019 05:18:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 05:18:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:30

Channel configuration set to: GFP: 12-Jul-2019 05:18:31

Channel:GFP set at:12-Jul-2019 05:18:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 05:18:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:34

Channel:Brightfield set at:12-Jul-2019 05:18:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 05:18:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:35

Channel configuration set to: GFP: 12-Jul-2019 05:18:36

Channel:GFP set at:12-Jul-2019 05:18:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 05:18:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:40

Channel:Brightfield set at:12-Jul-2019 05:18:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 05:18:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:40

Channel configuration set to: GFP: 12-Jul-2019 05:18:41

Channel:GFP set at:12-Jul-2019 05:18:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 05:18:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:45

Channel:Brightfield set at:12-Jul-2019 05:18:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 05:18:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:46

Channel configuration set to: GFP: 12-Jul-2019 05:18:47

Channel:GFP set at:12-Jul-2019 05:18:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 05:18:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:50

Channel:Brightfield set at:12-Jul-2019 05:18:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 12-Jul-2019 05:18:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:50

Channel configuration set to: GFP: 12-Jul-2019 05:18:52

Channel:GFP set at:12-Jul-2019 05:18:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 05:18:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 12-Jul-2019 05:18:55

Channel:Brightfield set at:12-Jul-2019 05:18:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 05:18:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:56

Channel configuration set to: GFP: 12-Jul-2019 05:18:57

Channel:GFP set at:12-Jul-2019 05:18:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 05:18:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:18:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:19:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 12-Jul-2019 05:19:00

Channel:Brightfield set at:12-Jul-2019 05:19:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 05:19:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:19:01

Channel configuration set to: GFP: 12-Jul-2019 05:19:02

Channel:GFP set at:12-Jul-2019 05:19:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 05:19:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:19:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:19:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.725

Channel configuration set to: Brightfield: 12-Jul-2019 05:19:06

Channel:Brightfield set at:12-Jul-2019 05:19:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 05:19:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:19:06

Channel configuration set to: GFP: 12-Jul-2019 05:19:07

Channel:GFP set at:12-Jul-2019 05:19:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 05:19:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:19:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:155 completed at:12-Jul-2019 05:19:10

Time since start of timelapse:46297.021

PFS is locked

Time to next time point:203.7063



------Time point_156------

Memory available for all arrays: 61023469568

Memory used by Matlab: 2259476480

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:22:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.975

Channel configuration set to: Brightfield: 12-Jul-2019 05:22:35

Channel:Brightfield set at:12-Jul-2019 05:22:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:22:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:22:36

Channel configuration set to: GFP: 12-Jul-2019 05:22:37

Channel:GFP set at:12-Jul-2019 05:22:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:22:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:22:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:22:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.05

Channel configuration set to: Brightfield: 12-Jul-2019 05:22:41

Channel:Brightfield set at:12-Jul-2019 05:22:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8001. 12-Jul-2019 05:22:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:22:41

Channel configuration set to: GFP: 12-Jul-2019 05:22:42

Channel:GFP set at:12-Jul-2019 05:22:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 12-Jul-2019 05:22:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:22:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:22:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 12-Jul-2019 05:22:45

Channel:Brightfield set at:12-Jul-2019 05:22:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 12-Jul-2019 05:22:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:22:46

Channel configuration set to: GFP: 12-Jul-2019 05:22:47

Channel:GFP set at:12-Jul-2019 05:22:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.8001. 12-Jul-2019 05:22:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:22:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:22:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.175

Channel configuration set to: Brightfield: 12-Jul-2019 05:22:51

Channel:Brightfield set at:12-Jul-2019 05:22:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 05:22:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:22:51

Channel configuration set to: GFP: 12-Jul-2019 05:22:52

Channel:GFP set at:12-Jul-2019 05:22:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 05:22:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:22:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:22:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 05:22:56

Channel:Brightfield set at:12-Jul-2019 05:22:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 05:22:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:22:57

Channel configuration set to: GFP: 12-Jul-2019 05:22:58

Channel:GFP set at:12-Jul-2019 05:22:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 05:22:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:22:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.325

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:02

Channel:Brightfield set at:12-Jul-2019 05:23:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:23:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:02

Channel configuration set to: GFP: 12-Jul-2019 05:23:03

Channel:GFP set at:12-Jul-2019 05:23:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 12-Jul-2019 05:23:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:08

Channel:Brightfield set at:12-Jul-2019 05:23:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 05:23:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:08

Channel configuration set to: GFP: 12-Jul-2019 05:23:09

Channel:GFP set at:12-Jul-2019 05:23:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 05:23:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.875

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:13

Channel:Brightfield set at:12-Jul-2019 05:23:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 12-Jul-2019 05:23:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:14

Channel configuration set to: GFP: 12-Jul-2019 05:23:15

Channel:GFP set at:12-Jul-2019 05:23:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 12-Jul-2019 05:23:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.3

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:19

Channel:Brightfield set at:12-Jul-2019 05:23:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 05:23:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:19

Channel configuration set to: GFP: 12-Jul-2019 05:23:20

Channel:GFP set at:12-Jul-2019 05:23:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 05:23:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.725

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:24

Channel:Brightfield set at:12-Jul-2019 05:23:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 05:23:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:25

Channel configuration set to: GFP: 12-Jul-2019 05:23:26

Channel:GFP set at:12-Jul-2019 05:23:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2001. 12-Jul-2019 05:23:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:29

Channel:Brightfield set at:12-Jul-2019 05:23:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8751. 12-Jul-2019 05:23:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:30

Channel configuration set to: GFP: 12-Jul-2019 05:23:31

Channel:GFP set at:12-Jul-2019 05:23:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8751. 12-Jul-2019 05:23:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:35

Channel:Brightfield set at:12-Jul-2019 05:23:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 05:23:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:35

Channel configuration set to: GFP: 12-Jul-2019 05:23:36

Channel:GFP set at:12-Jul-2019 05:23:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 05:23:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:40

Channel:Brightfield set at:12-Jul-2019 05:23:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 05:23:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:41

Channel configuration set to: GFP: 12-Jul-2019 05:23:42

Channel:GFP set at:12-Jul-2019 05:23:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 05:23:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:46

Channel:Brightfield set at:12-Jul-2019 05:23:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 05:23:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:46

Channel configuration set to: GFP: 12-Jul-2019 05:23:48

Channel:GFP set at:12-Jul-2019 05:23:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 05:23:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:51

Channel:Brightfield set at:12-Jul-2019 05:23:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 05:23:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:52

Channel configuration set to: GFP: 12-Jul-2019 05:23:53

Channel:GFP set at:12-Jul-2019 05:23:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 05:23:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:54

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.725

Channel configuration set to: Brightfield: 12-Jul-2019 05:23:57

Channel:Brightfield set at:12-Jul-2019 05:23:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 05:23:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:58

Channel configuration set to: GFP: 12-Jul-2019 05:23:59

Channel:GFP set at:12-Jul-2019 05:23:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 05:23:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:23:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:24:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.3

Channel configuration set to: Brightfield: 12-Jul-2019 05:24:02

Channel:Brightfield set at:12-Jul-2019 05:24:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 12-Jul-2019 05:24:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:24:03

Channel configuration set to: GFP: 12-Jul-2019 05:24:04

Channel:GFP set at:12-Jul-2019 05:24:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 12-Jul-2019 05:24:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:24:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:24:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 05:24:08

Channel:Brightfield set at:12-Jul-2019 05:24:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 05:24:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:24:08

Channel configuration set to: GFP: 12-Jul-2019 05:24:09

Channel:GFP set at:12-Jul-2019 05:24:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 05:24:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:24:10

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:156 completed at:12-Jul-2019 05:24:12

Time since start of timelapse:46599.17

PFS is locked

Time to next time point:201.5585



------Time point_157------

Memory available for all arrays: 60998332416

Memory used by Matlab: 2262364160

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:27:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.95

Channel configuration set to: Brightfield: 12-Jul-2019 05:27:35

Channel:Brightfield set at:12-Jul-2019 05:27:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:27:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:27:36

Channel configuration set to: GFP: 12-Jul-2019 05:27:37

Channel:GFP set at:12-Jul-2019 05:27:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:27:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:27:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:27:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.125

Channel configuration set to: Brightfield: 12-Jul-2019 05:27:41

Channel:Brightfield set at:12-Jul-2019 05:27:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7251. 12-Jul-2019 05:27:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:27:41

Channel configuration set to: GFP: 12-Jul-2019 05:27:43

Channel:GFP set at:12-Jul-2019 05:27:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7251. 12-Jul-2019 05:27:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:27:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:27:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 12-Jul-2019 05:27:46

Channel:Brightfield set at:12-Jul-2019 05:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 05:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:27:46

Channel configuration set to: GFP: 12-Jul-2019 05:27:47

Channel:GFP set at:12-Jul-2019 05:27:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5501. 12-Jul-2019 05:27:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:27:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:27:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.175

Channel configuration set to: Brightfield: 12-Jul-2019 05:27:51

Channel:Brightfield set at:12-Jul-2019 05:27:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 05:27:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:27:51

Channel configuration set to: GFP: 12-Jul-2019 05:27:53

Channel:GFP set at:12-Jul-2019 05:27:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 05:27:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:27:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:27:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.225

Channel configuration set to: Brightfield: 12-Jul-2019 05:27:56

Channel:Brightfield set at:12-Jul-2019 05:27:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 05:27:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:27:57

Channel configuration set to: GFP: 12-Jul-2019 05:27:58

Channel:GFP set at:12-Jul-2019 05:27:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 05:27:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:27:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.55

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:02

Channel:Brightfield set at:12-Jul-2019 05:28:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:02

Channel configuration set to: GFP: 12-Jul-2019 05:28:04

Channel:GFP set at:12-Jul-2019 05:28:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:28:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:08

Channel:Brightfield set at:12-Jul-2019 05:28:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 05:28:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:08

Channel configuration set to: GFP: 12-Jul-2019 05:28:10

Channel:GFP set at:12-Jul-2019 05:28:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 05:28:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:13

Channel:Brightfield set at:12-Jul-2019 05:28:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 05:28:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:13

Channel configuration set to: GFP: 12-Jul-2019 05:28:15

Channel:GFP set at:12-Jul-2019 05:28:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 05:28:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:18

Channel:Brightfield set at:12-Jul-2019 05:28:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 05:28:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:19

Channel configuration set to: GFP: 12-Jul-2019 05:28:20

Channel:GFP set at:12-Jul-2019 05:28:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 05:28:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.8

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:24

Channel:Brightfield set at:12-Jul-2019 05:28:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 05:28:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:24

Channel configuration set to: GFP: 12-Jul-2019 05:28:25

Channel:GFP set at:12-Jul-2019 05:28:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 05:28:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:30

Channel:Brightfield set at:12-Jul-2019 05:28:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 05:28:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:30

Channel configuration set to: GFP: 12-Jul-2019 05:28:31

Channel:GFP set at:12-Jul-2019 05:28:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 05:28:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:35

Channel:Brightfield set at:12-Jul-2019 05:28:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 05:28:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:35

Channel configuration set to: GFP: 12-Jul-2019 05:28:36

Channel:GFP set at:12-Jul-2019 05:28:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 05:28:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:41

Channel:Brightfield set at:12-Jul-2019 05:28:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:28:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:41

Channel configuration set to: GFP: 12-Jul-2019 05:28:42

Channel:GFP set at:12-Jul-2019 05:28:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:28:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:46

Channel:Brightfield set at:12-Jul-2019 05:28:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 05:28:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:47

Channel configuration set to: GFP: 12-Jul-2019 05:28:48

Channel:GFP set at:12-Jul-2019 05:28:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 05:28:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:51

Channel:Brightfield set at:12-Jul-2019 05:28:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 05:28:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:52

Channel configuration set to: GFP: 12-Jul-2019 05:28:53

Channel:GFP set at:12-Jul-2019 05:28:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 05:28:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-7.9

Channel configuration set to: Brightfield: 12-Jul-2019 05:28:56

Channel:Brightfield set at:12-Jul-2019 05:28:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9501. 12-Jul-2019 05:28:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:57

Channel configuration set to: GFP: 12-Jul-2019 05:28:58

Channel:GFP set at:12-Jul-2019 05:28:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 12-Jul-2019 05:28:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:28:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:29:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.275

Channel configuration set to: Brightfield: 12-Jul-2019 05:29:02

Channel:Brightfield set at:12-Jul-2019 05:29:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5001. 12-Jul-2019 05:29:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:29:02

Channel configuration set to: GFP: 12-Jul-2019 05:29:03

Channel:GFP set at:12-Jul-2019 05:29:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5251. 12-Jul-2019 05:29:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:29:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:29:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-5

Channel configuration set to: Brightfield: 12-Jul-2019 05:29:07

Channel:Brightfield set at:12-Jul-2019 05:29:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 12-Jul-2019 05:29:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:29:07

Channel configuration set to: GFP: 12-Jul-2019 05:29:09

Channel:GFP set at:12-Jul-2019 05:29:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.9001. 12-Jul-2019 05:29:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:29:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:157 completed at:12-Jul-2019 05:29:11

Time since start of timelapse:46898.73

PFS is locked

Time to next time point:202.0001



------Time point_158------

Memory available for all arrays: 61013434368

Memory used by Matlab: 2262364160

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:32:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.8

Channel configuration set to: Brightfield: 12-Jul-2019 05:32:35

Channel:Brightfield set at:12-Jul-2019 05:32:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 12-Jul-2019 05:32:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:32:35

Channel configuration set to: GFP: 12-Jul-2019 05:32:36

Channel:GFP set at:12-Jul-2019 05:32:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 12-Jul-2019 05:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:32:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:32:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-11.275

Channel configuration set to: Brightfield: 12-Jul-2019 05:32:40

Channel:Brightfield set at:12-Jul-2019 05:32:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 12-Jul-2019 05:32:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:32:40

Channel configuration set to: GFP: 12-Jul-2019 05:32:42

Channel:GFP set at:12-Jul-2019 05:32:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 12-Jul-2019 05:32:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:32:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:32:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.35

Channel configuration set to: Brightfield: 12-Jul-2019 05:32:45

Channel:Brightfield set at:12-Jul-2019 05:32:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 05:32:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:32:45

Channel configuration set to: GFP: 12-Jul-2019 05:32:47

Channel:GFP set at:12-Jul-2019 05:32:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5751. 12-Jul-2019 05:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:32:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:32:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.075

Channel configuration set to: Brightfield: 12-Jul-2019 05:32:50

Channel:Brightfield set at:12-Jul-2019 05:32:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 05:32:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:32:51

Channel configuration set to: GFP: 12-Jul-2019 05:32:52

Channel:GFP set at:12-Jul-2019 05:32:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 05:32:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:32:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:32:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.175

Channel configuration set to: Brightfield: 12-Jul-2019 05:32:56

Channel:Brightfield set at:12-Jul-2019 05:32:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 05:32:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:32:56

Channel configuration set to: GFP: 12-Jul-2019 05:32:57

Channel:GFP set at:12-Jul-2019 05:32:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 05:32:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:32:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.45

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:01

Channel:Brightfield set at:12-Jul-2019 05:33:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 05:33:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:02

Channel configuration set to: GFP: 12-Jul-2019 05:33:03

Channel:GFP set at:12-Jul-2019 05:33:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 05:33:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:07

Channel:Brightfield set at:12-Jul-2019 05:33:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 05:33:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:08

Channel configuration set to: GFP: 12-Jul-2019 05:33:09

Channel:GFP set at:12-Jul-2019 05:33:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 05:33:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.9

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:12

Channel:Brightfield set at:12-Jul-2019 05:33:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 12-Jul-2019 05:33:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:13

Channel configuration set to: GFP: 12-Jul-2019 05:33:14

Channel:GFP set at:12-Jul-2019 05:33:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 12-Jul-2019 05:33:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.2

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:18

Channel:Brightfield set at:12-Jul-2019 05:33:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:33:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:18

Channel configuration set to: GFP: 12-Jul-2019 05:33:20

Channel:GFP set at:12-Jul-2019 05:33:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:33:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.8

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:23

Channel:Brightfield set at:12-Jul-2019 05:33:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 05:33:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:24

Channel configuration set to: GFP: 12-Jul-2019 05:33:25

Channel:GFP set at:12-Jul-2019 05:33:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 05:33:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:29

Channel:Brightfield set at:12-Jul-2019 05:33:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 05:33:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:29

Channel configuration set to: GFP: 12-Jul-2019 05:33:31

Channel:GFP set at:12-Jul-2019 05:33:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 05:33:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:34

Channel:Brightfield set at:12-Jul-2019 05:33:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 05:33:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:35

Channel configuration set to: GFP: 12-Jul-2019 05:33:36

Channel:GFP set at:12-Jul-2019 05:33:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 05:33:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.65

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:40

Channel:Brightfield set at:12-Jul-2019 05:33:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 05:33:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:40

Channel configuration set to: GFP: 12-Jul-2019 05:33:41

Channel:GFP set at:12-Jul-2019 05:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 05:33:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:45

Channel:Brightfield set at:12-Jul-2019 05:33:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 05:33:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:46

Channel configuration set to: GFP: 12-Jul-2019 05:33:47

Channel:GFP set at:12-Jul-2019 05:33:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 05:33:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.75

PFS not locked. Waiting for lock...

PFS took 0.200000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:51

Channel:Brightfield set at:12-Jul-2019 05:33:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:33:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:51

Channel configuration set to: GFP: 12-Jul-2019 05:33:52

Channel:GFP set at:12-Jul-2019 05:33:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:33:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 12-Jul-2019 05:33:56

Channel:Brightfield set at:12-Jul-2019 05:33:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 05:33:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:56

Channel configuration set to: GFP: 12-Jul-2019 05:33:58

Channel:GFP set at:12-Jul-2019 05:33:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 05:33:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:33:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:34:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.4

Channel configuration set to: Brightfield: 12-Jul-2019 05:34:01

Channel:Brightfield set at:12-Jul-2019 05:34:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 05:34:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:34:02

Channel configuration set to: GFP: 12-Jul-2019 05:34:03

Channel:GFP set at:12-Jul-2019 05:34:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 12-Jul-2019 05:34:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:34:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:34:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.65

Channel configuration set to: Brightfield: 12-Jul-2019 05:34:06

Channel:Brightfield set at:12-Jul-2019 05:34:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 05:34:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:34:07

Channel configuration set to: GFP: 12-Jul-2019 05:34:08

Channel:GFP set at:12-Jul-2019 05:34:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 05:34:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:34:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:158 completed at:12-Jul-2019 05:34:10

Time since start of timelapse:47197.7662

PFS is locked

Time to next time point:202.9653



------Time point_159------

Memory available for all arrays: 61015896064

Memory used by Matlab: 2262364160

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:37:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-9.925

Channel configuration set to: Brightfield: 12-Jul-2019 05:37:35

Channel:Brightfield set at:12-Jul-2019 05:37:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 05:37:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:37:36

Channel configuration set to: GFP: 12-Jul-2019 05:37:37

Channel:GFP set at:12-Jul-2019 05:37:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 05:37:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:37:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:37:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.175

Channel configuration set to: Brightfield: 12-Jul-2019 05:37:41

Channel:Brightfield set at:12-Jul-2019 05:37:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 05:37:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:37:42

Channel configuration set to: GFP: 12-Jul-2019 05:37:43

Channel:GFP set at:12-Jul-2019 05:37:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7001. 12-Jul-2019 05:37:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:37:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:37:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.175

Channel configuration set to: Brightfield: 12-Jul-2019 05:37:46

Channel:Brightfield set at:12-Jul-2019 05:37:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 12-Jul-2019 05:37:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:37:47

Channel configuration set to: GFP: 12-Jul-2019 05:37:48

Channel:GFP set at:12-Jul-2019 05:37:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8251. 12-Jul-2019 05:37:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:37:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:37:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.275

Channel configuration set to: Brightfield: 12-Jul-2019 05:37:52

Channel:Brightfield set at:12-Jul-2019 05:37:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:37:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:37:52

Channel configuration set to: GFP: 12-Jul-2019 05:37:53

Channel:GFP set at:12-Jul-2019 05:37:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:37:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:37:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:37:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 05:37:57

Channel:Brightfield set at:12-Jul-2019 05:37:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 05:37:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:37:58

Channel configuration set to: GFP: 12-Jul-2019 05:37:59

Channel:GFP set at:12-Jul-2019 05:37:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 05:37:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:37:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.6

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:02

Channel:Brightfield set at:12-Jul-2019 05:38:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 05:38:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:03

Channel configuration set to: GFP: 12-Jul-2019 05:38:04

Channel:GFP set at:12-Jul-2019 05:38:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 12-Jul-2019 05:38:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:08

Channel:Brightfield set at:12-Jul-2019 05:38:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 05:38:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:08

Channel configuration set to: GFP: 12-Jul-2019 05:38:10

Channel:GFP set at:12-Jul-2019 05:38:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 05:38:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:12

Channel:Brightfield set at:12-Jul-2019 05:38:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:38:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:13

Channel configuration set to: GFP: 12-Jul-2019 05:38:14

Channel:GFP set at:12-Jul-2019 05:38:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 05:38:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.15

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:18

Channel:Brightfield set at:12-Jul-2019 05:38:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3501. 12-Jul-2019 05:38:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:18

Channel configuration set to: GFP: 12-Jul-2019 05:38:19

Channel:GFP set at:12-Jul-2019 05:38:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3251. 12-Jul-2019 05:38:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:23

Channel:Brightfield set at:12-Jul-2019 05:38:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 05:38:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:24

Channel configuration set to: GFP: 12-Jul-2019 05:38:25

Channel:GFP set at:12-Jul-2019 05:38:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 05:38:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:29

Channel:Brightfield set at:12-Jul-2019 05:38:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 05:38:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:29

Channel configuration set to: GFP: 12-Jul-2019 05:38:31

Channel:GFP set at:12-Jul-2019 05:38:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 05:38:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.3

PFS not locked. Waiting for lock...

PFS took 0.900000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:35

Channel:Brightfield set at:12-Jul-2019 05:38:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4251. 12-Jul-2019 05:38:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:35

Channel configuration set to: GFP: 12-Jul-2019 05:38:37

Channel:GFP set at:12-Jul-2019 05:38:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4501. 12-Jul-2019 05:38:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:40

Channel:Brightfield set at:12-Jul-2019 05:38:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 05:38:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:41

Channel configuration set to: GFP: 12-Jul-2019 05:38:42

Channel:GFP set at:12-Jul-2019 05:38:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 05:38:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:46

Channel:Brightfield set at:12-Jul-2019 05:38:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 05:38:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:46

Channel configuration set to: GFP: 12-Jul-2019 05:38:48

Channel:GFP set at:12-Jul-2019 05:38:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 05:38:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.775

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:52

Channel:Brightfield set at:12-Jul-2019 05:38:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 05:38:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:52

Channel configuration set to: GFP: 12-Jul-2019 05:38:53

Channel:GFP set at:12-Jul-2019 05:38:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 05:38:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:54

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 12-Jul-2019 05:38:57

Channel:Brightfield set at:12-Jul-2019 05:38:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 05:38:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:58

Channel configuration set to: GFP: 12-Jul-2019 05:38:59

Channel:GFP set at:12-Jul-2019 05:38:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 05:38:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:38:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:39:02

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 12-Jul-2019 05:39:03

Channel:Brightfield set at:12-Jul-2019 05:39:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3501. 12-Jul-2019 05:39:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:39:03

Channel configuration set to: GFP: 12-Jul-2019 05:39:04

Channel:GFP set at:12-Jul-2019 05:39:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 12-Jul-2019 05:39:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:39:05

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:39:07

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 05:39:08

Channel:Brightfield set at:12-Jul-2019 05:39:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 05:39:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:39:09

Channel configuration set to: GFP: 12-Jul-2019 05:39:10

Channel:GFP set at:12-Jul-2019 05:39:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 05:39:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:39:10

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:159 completed at:12-Jul-2019 05:39:12

Time since start of timelapse:47499.8188

PFS is locked

Time to next time point:200.9141



------Time point_160------

Memory available for all arrays: 61012832256

Memory used by Matlab: 2262364160

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:42:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.225

Channel configuration set to: Brightfield: 12-Jul-2019 05:42:35

Channel:Brightfield set at:12-Jul-2019 05:42:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 05:42:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:42:36

Channel configuration set to: GFP: 12-Jul-2019 05:42:37

Channel:GFP set at:12-Jul-2019 05:42:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 05:42:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:42:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:42:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 05:42:41

Channel:Brightfield set at:12-Jul-2019 05:42:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 05:42:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:42:41

Channel configuration set to: GFP: 12-Jul-2019 05:42:42

Channel:GFP set at:12-Jul-2019 05:42:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 05:42:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:42:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:42:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.175

Channel configuration set to: Brightfield: 12-Jul-2019 05:42:46

Channel:Brightfield set at:12-Jul-2019 05:42:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 12-Jul-2019 05:42:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:42:46

Channel configuration set to: GFP: 12-Jul-2019 05:42:48

Channel:GFP set at:12-Jul-2019 05:42:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 12-Jul-2019 05:42:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:42:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:42:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.75

Channel configuration set to: Brightfield: 12-Jul-2019 05:42:51

Channel:Brightfield set at:12-Jul-2019 05:42:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 05:42:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:42:52

Channel configuration set to: GFP: 12-Jul-2019 05:42:53

Channel:GFP set at:12-Jul-2019 05:42:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 05:42:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:42:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:42:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.625

Channel configuration set to: Brightfield: 12-Jul-2019 05:42:57

Channel:Brightfield set at:12-Jul-2019 05:42:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3751. 12-Jul-2019 05:42:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:42:57

Channel configuration set to: GFP: 12-Jul-2019 05:42:58

Channel:GFP set at:12-Jul-2019 05:42:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.3501. 12-Jul-2019 05:42:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:42:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.65

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:03

Channel:Brightfield set at:12-Jul-2019 05:43:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 05:43:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:03

Channel configuration set to: GFP: 12-Jul-2019 05:43:04

Channel:GFP set at:12-Jul-2019 05:43:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 05:43:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:08

Channel:Brightfield set at:12-Jul-2019 05:43:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:43:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:08

Channel configuration set to: GFP: 12-Jul-2019 05:43:10

Channel:GFP set at:12-Jul-2019 05:43:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 05:43:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:13

Channel:Brightfield set at:12-Jul-2019 05:43:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 05:43:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:13

Channel configuration set to: GFP: 12-Jul-2019 05:43:15

Channel:GFP set at:12-Jul-2019 05:43:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 05:43:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.45

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:19

Channel:Brightfield set at:12-Jul-2019 05:43:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 05:43:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:19

Channel configuration set to: GFP: 12-Jul-2019 05:43:21

Channel:GFP set at:12-Jul-2019 05:43:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 05:43:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.9

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:24

Channel:Brightfield set at:12-Jul-2019 05:43:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 05:43:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:24

Channel configuration set to: GFP: 12-Jul-2019 05:43:25

Channel:GFP set at:12-Jul-2019 05:43:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 05:43:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:29

Channel:Brightfield set at:12-Jul-2019 05:43:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5501. 12-Jul-2019 05:43:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:29

Channel configuration set to: GFP: 12-Jul-2019 05:43:31

Channel:GFP set at:12-Jul-2019 05:43:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5501. 12-Jul-2019 05:43:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:34

Channel:Brightfield set at:12-Jul-2019 05:43:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 05:43:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:34

Channel configuration set to: GFP: 12-Jul-2019 05:43:36

Channel:GFP set at:12-Jul-2019 05:43:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 05:43:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:40

Channel:Brightfield set at:12-Jul-2019 05:43:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 05:43:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:40

Channel configuration set to: GFP: 12-Jul-2019 05:43:42

Channel:GFP set at:12-Jul-2019 05:43:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 05:43:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:46

Channel:Brightfield set at:12-Jul-2019 05:43:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 05:43:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:46

Channel configuration set to: GFP: 12-Jul-2019 05:43:47

Channel:GFP set at:12-Jul-2019 05:43:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 05:43:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.025

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:50

Channel:Brightfield set at:12-Jul-2019 05:43:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 05:43:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:50

Channel configuration set to: GFP: 12-Jul-2019 05:43:52

Channel:GFP set at:12-Jul-2019 05:43:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 05:43:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 12-Jul-2019 05:43:56

Channel:Brightfield set at:12-Jul-2019 05:43:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 05:43:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:56

Channel configuration set to: GFP: 12-Jul-2019 05:43:57

Channel:GFP set at:12-Jul-2019 05:43:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 05:43:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:43:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:44:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 12-Jul-2019 05:44:01

Channel:Brightfield set at:12-Jul-2019 05:44:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 12-Jul-2019 05:44:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:44:01

Channel configuration set to: GFP: 12-Jul-2019 05:44:03

Channel:GFP set at:12-Jul-2019 05:44:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1001. 12-Jul-2019 05:44:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:44:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:44:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.8

Channel configuration set to: Brightfield: 12-Jul-2019 05:44:06

Channel:Brightfield set at:12-Jul-2019 05:44:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 05:44:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:44:07

Channel configuration set to: GFP: 12-Jul-2019 05:44:08

Channel:GFP set at:12-Jul-2019 05:44:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 05:44:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:44:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:160 completed at:12-Jul-2019 05:44:10

Time since start of timelapse:47797.7582

PFS is locked

Time to next time point:202.9764



------Time point_161------

Memory available for all arrays: 61004480512

Memory used by Matlab: 2262364160

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:47:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.325

Channel configuration set to: Brightfield: 12-Jul-2019 05:47:35

Channel:Brightfield set at:12-Jul-2019 05:47:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 05:47:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:47:36

Channel configuration set to: GFP: 12-Jul-2019 05:47:37

Channel:GFP set at:12-Jul-2019 05:47:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 05:47:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:47:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:47:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.45

Channel configuration set to: Brightfield: 12-Jul-2019 05:47:41

Channel:Brightfield set at:12-Jul-2019 05:47:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 05:47:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:47:41

Channel configuration set to: GFP: 12-Jul-2019 05:47:42

Channel:GFP set at:12-Jul-2019 05:47:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4751. 12-Jul-2019 05:47:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:47:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:47:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.475

Channel configuration set to: Brightfield: 12-Jul-2019 05:47:45

Channel:Brightfield set at:12-Jul-2019 05:47:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 12-Jul-2019 05:47:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:47:46

Channel configuration set to: GFP: 12-Jul-2019 05:47:47

Channel:GFP set at:12-Jul-2019 05:47:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 12-Jul-2019 05:47:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:47:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:47:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.625

PFS not locked. Waiting for lock...

PFS took 0.250000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:47:51

Channel:Brightfield set at:12-Jul-2019 05:47:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 05:47:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:47:51

Channel configuration set to: GFP: 12-Jul-2019 05:47:52

Channel:GFP set at:12-Jul-2019 05:47:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 05:47:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:47:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:47:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.825

Channel configuration set to: Brightfield: 12-Jul-2019 05:47:56

Channel:Brightfield set at:12-Jul-2019 05:47:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 05:47:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:47:57

Channel configuration set to: GFP: 12-Jul-2019 05:47:58

Channel:GFP set at:12-Jul-2019 05:47:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 05:47:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:47:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.275

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:01

Channel:Brightfield set at:12-Jul-2019 05:48:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 05:48:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:02

Channel configuration set to: GFP: 12-Jul-2019 05:48:03

Channel:GFP set at:12-Jul-2019 05:48:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 05:48:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:07

Channel:Brightfield set at:12-Jul-2019 05:48:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 05:48:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:07

Channel configuration set to: GFP: 12-Jul-2019 05:48:08

Channel:GFP set at:12-Jul-2019 05:48:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 05:48:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.925

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:12

Channel:Brightfield set at:12-Jul-2019 05:48:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 05:48:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:12

Channel configuration set to: GFP: 12-Jul-2019 05:48:14

Channel:GFP set at:12-Jul-2019 05:48:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 05:48:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:18

Channel:Brightfield set at:12-Jul-2019 05:48:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 05:48:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:18

Channel configuration set to: GFP: 12-Jul-2019 05:48:20

Channel:GFP set at:12-Jul-2019 05:48:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 05:48:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:23

Channel:Brightfield set at:12-Jul-2019 05:48:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 05:48:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:23

Channel configuration set to: GFP: 12-Jul-2019 05:48:25

Channel:GFP set at:12-Jul-2019 05:48:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 05:48:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:28

Channel:Brightfield set at:12-Jul-2019 05:48:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 05:48:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:29

Channel configuration set to: GFP: 12-Jul-2019 05:48:30

Channel:GFP set at:12-Jul-2019 05:48:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 05:48:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:33

Channel:Brightfield set at:12-Jul-2019 05:48:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 05:48:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:34

Channel configuration set to: GFP: 12-Jul-2019 05:48:35

Channel:GFP set at:12-Jul-2019 05:48:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 05:48:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:39

Channel:Brightfield set at:12-Jul-2019 05:48:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 05:48:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:40

Channel configuration set to: GFP: 12-Jul-2019 05:48:41

Channel:GFP set at:12-Jul-2019 05:48:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 05:48:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:45

Channel:Brightfield set at:12-Jul-2019 05:48:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 05:48:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:45

Channel configuration set to: GFP: 12-Jul-2019 05:48:47

Channel:GFP set at:12-Jul-2019 05:48:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:48:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:49

Channel:Brightfield set at:12-Jul-2019 05:48:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 05:48:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:50

Channel configuration set to: GFP: 12-Jul-2019 05:48:51

Channel:GFP set at:12-Jul-2019 05:48:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 05:48:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.075

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:55

Channel:Brightfield set at:12-Jul-2019 05:48:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3251. 12-Jul-2019 05:48:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:55

Channel configuration set to: GFP: 12-Jul-2019 05:48:56

Channel:GFP set at:12-Jul-2019 05:48:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3251. 12-Jul-2019 05:48:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:48:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:48:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 12-Jul-2019 05:49:00

Channel:Brightfield set at:12-Jul-2019 05:49:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 05:49:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:49:01

Channel configuration set to: GFP: 12-Jul-2019 05:49:02

Channel:GFP set at:12-Jul-2019 05:49:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8251. 12-Jul-2019 05:49:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:49:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:49:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 12-Jul-2019 05:49:05

Channel:Brightfield set at:12-Jul-2019 05:49:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:49:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:49:06

Channel configuration set to: GFP: 12-Jul-2019 05:49:07

Channel:GFP set at:12-Jul-2019 05:49:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:49:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:49:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:161 completed at:12-Jul-2019 05:49:10

Time since start of timelapse:48097.0565

PFS is locked

Time to next time point:203.6798



------Time point_162------

Memory available for all arrays: 61012414464

Memory used by Matlab: 2262687744

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:52:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.375

Channel configuration set to: Brightfield: 12-Jul-2019 05:52:35

Channel:Brightfield set at:12-Jul-2019 05:52:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 05:52:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:52:36

Channel configuration set to: GFP: 12-Jul-2019 05:52:37

Channel:GFP set at:12-Jul-2019 05:52:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 05:52:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:52:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:52:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.65

Channel configuration set to: Brightfield: 12-Jul-2019 05:52:41

Channel:Brightfield set at:12-Jul-2019 05:52:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 12-Jul-2019 05:52:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:52:41

Channel configuration set to: GFP: 12-Jul-2019 05:52:42

Channel:GFP set at:12-Jul-2019 05:52:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 12-Jul-2019 05:52:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:52:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:52:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.575

Channel configuration set to: Brightfield: 12-Jul-2019 05:52:46

Channel:Brightfield set at:12-Jul-2019 05:52:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 12-Jul-2019 05:52:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:52:46

Channel configuration set to: GFP: 12-Jul-2019 05:52:47

Channel:GFP set at:12-Jul-2019 05:52:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2501. 12-Jul-2019 05:52:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:52:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:52:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.8

Channel configuration set to: Brightfield: 12-Jul-2019 05:52:51

Channel:Brightfield set at:12-Jul-2019 05:52:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 05:52:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:52:51

Channel configuration set to: GFP: 12-Jul-2019 05:52:53

Channel:GFP set at:12-Jul-2019 05:52:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 05:52:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:52:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:52:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.5

Channel configuration set to: Brightfield: 12-Jul-2019 05:52:56

Channel:Brightfield set at:12-Jul-2019 05:52:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 05:52:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:52:56

Channel configuration set to: GFP: 12-Jul-2019 05:52:58

Channel:GFP set at:12-Jul-2019 05:52:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 05:52:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:52:58

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.275

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:02

Channel:Brightfield set at:12-Jul-2019 05:53:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 05:53:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:02

Channel configuration set to: GFP: 12-Jul-2019 05:53:04

Channel:GFP set at:12-Jul-2019 05:53:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 12-Jul-2019 05:53:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:07

Channel:Brightfield set at:12-Jul-2019 05:53:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 05:53:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:07

Channel configuration set to: GFP: 12-Jul-2019 05:53:09

Channel:GFP set at:12-Jul-2019 05:53:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 05:53:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:12

Channel:Brightfield set at:12-Jul-2019 05:53:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 05:53:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:13

Channel configuration set to: GFP: 12-Jul-2019 05:53:14

Channel:GFP set at:12-Jul-2019 05:53:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 05:53:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.95

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:17

Channel:Brightfield set at:12-Jul-2019 05:53:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 05:53:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:18

Channel configuration set to: GFP: 12-Jul-2019 05:53:19

Channel:GFP set at:12-Jul-2019 05:53:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 05:53:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:21

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.225

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:23

Channel:Brightfield set at:12-Jul-2019 05:53:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7001. 12-Jul-2019 05:53:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:23

Channel configuration set to: GFP: 12-Jul-2019 05:53:24

Channel:GFP set at:12-Jul-2019 05:53:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7251. 12-Jul-2019 05:53:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.475

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:28

Channel:Brightfield set at:12-Jul-2019 05:53:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6001. 12-Jul-2019 05:53:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:29

Channel configuration set to: GFP: 12-Jul-2019 05:53:30

Channel:GFP set at:12-Jul-2019 05:53:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6001. 12-Jul-2019 05:53:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:33

Channel:Brightfield set at:12-Jul-2019 05:53:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3751. 12-Jul-2019 05:53:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:34

Channel configuration set to: GFP: 12-Jul-2019 05:53:35

Channel:GFP set at:12-Jul-2019 05:53:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3501. 12-Jul-2019 05:53:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:40

Channel:Brightfield set at:12-Jul-2019 05:53:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 05:53:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:40

Channel configuration set to: GFP: 12-Jul-2019 05:53:41

Channel:GFP set at:12-Jul-2019 05:53:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 05:53:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:45

Channel:Brightfield set at:12-Jul-2019 05:53:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:53:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:46

Channel configuration set to: GFP: 12-Jul-2019 05:53:47

Channel:GFP set at:12-Jul-2019 05:53:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:53:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.2

PFS not locked. Waiting for lock...

PFS took 0.250000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:50

Channel:Brightfield set at:12-Jul-2019 05:53:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 05:53:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:51

Channel configuration set to: GFP: 12-Jul-2019 05:53:52

Channel:GFP set at:12-Jul-2019 05:53:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 05:53:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 12-Jul-2019 05:53:56

Channel:Brightfield set at:12-Jul-2019 05:53:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 12-Jul-2019 05:53:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:56

Channel configuration set to: GFP: 12-Jul-2019 05:53:58

Channel:GFP set at:12-Jul-2019 05:53:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 12-Jul-2019 05:53:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:53:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:54:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 12-Jul-2019 05:54:02

Channel:Brightfield set at:12-Jul-2019 05:54:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 05:54:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:54:02

Channel configuration set to: GFP: 12-Jul-2019 05:54:03

Channel:GFP set at:12-Jul-2019 05:54:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 05:54:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:54:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:54:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 12-Jul-2019 05:54:07

Channel:Brightfield set at:12-Jul-2019 05:54:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 05:54:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:54:07

Channel configuration set to: GFP: 12-Jul-2019 05:54:08

Channel:GFP set at:12-Jul-2019 05:54:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 05:54:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:54:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:162 completed at:12-Jul-2019 05:54:11

Time since start of timelapse:48398.4987

PFS is locked

Time to next time point:202.2388



------Time point_163------

Memory available for all arrays: 61011775488

Memory used by Matlab: 2263080960

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:57:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.925

Channel configuration set to: Brightfield: 12-Jul-2019 05:57:35

Channel:Brightfield set at:12-Jul-2019 05:57:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 05:57:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:57:35

Channel configuration set to: GFP: 12-Jul-2019 05:57:36

Channel:GFP set at:12-Jul-2019 05:57:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 05:57:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:57:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:57:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.45

Channel configuration set to: Brightfield: 12-Jul-2019 05:57:40

Channel:Brightfield set at:12-Jul-2019 05:57:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4251. 12-Jul-2019 05:57:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:57:41

Channel configuration set to: GFP: 12-Jul-2019 05:57:42

Channel:GFP set at:12-Jul-2019 05:57:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4251. 12-Jul-2019 05:57:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:57:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:57:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.525

Channel configuration set to: Brightfield: 12-Jul-2019 05:57:46

Channel:Brightfield set at:12-Jul-2019 05:57:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 05:57:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:57:46

Channel configuration set to: GFP: 12-Jul-2019 05:57:48

Channel:GFP set at:12-Jul-2019 05:57:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 05:57:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:57:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:57:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.85

Channel configuration set to: Brightfield: 12-Jul-2019 05:57:51

Channel:Brightfield set at:12-Jul-2019 05:57:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 05:57:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:57:52

Channel configuration set to: GFP: 12-Jul-2019 05:57:53

Channel:GFP set at:12-Jul-2019 05:57:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 05:57:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:57:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:57:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.9

Channel configuration set to: Brightfield: 12-Jul-2019 05:57:57

Channel:Brightfield set at:12-Jul-2019 05:57:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 12-Jul-2019 05:57:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:57:57

Channel configuration set to: GFP: 12-Jul-2019 05:57:59

Channel:GFP set at:12-Jul-2019 05:57:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1001. 12-Jul-2019 05:57:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:57:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.2

PFS not locked. Waiting for lock...

PFS took 0.150000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:02

Channel:Brightfield set at:12-Jul-2019 05:58:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 12-Jul-2019 05:58:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:02

Channel configuration set to: GFP: 12-Jul-2019 05:58:04

Channel:GFP set at:12-Jul-2019 05:58:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6501. 12-Jul-2019 05:58:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.7

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:08

Channel:Brightfield set at:12-Jul-2019 05:58:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 12-Jul-2019 05:58:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:08

Channel configuration set to: GFP: 12-Jul-2019 05:58:09

Channel:GFP set at:12-Jul-2019 05:58:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 05:58:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:13

Channel:Brightfield set at:12-Jul-2019 05:58:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 05:58:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:14

Channel configuration set to: GFP: 12-Jul-2019 05:58:15

Channel:GFP set at:12-Jul-2019 05:58:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 12-Jul-2019 05:58:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.55

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:19

Channel:Brightfield set at:12-Jul-2019 05:58:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5501. 12-Jul-2019 05:58:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:19

Channel configuration set to: GFP: 12-Jul-2019 05:58:21

Channel:GFP set at:12-Jul-2019 05:58:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5501. 12-Jul-2019 05:58:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:24

Channel:Brightfield set at:12-Jul-2019 05:58:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 12-Jul-2019 05:58:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:24

Channel configuration set to: GFP: 12-Jul-2019 05:58:25

Channel:GFP set at:12-Jul-2019 05:58:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 12-Jul-2019 05:58:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:29

Channel:Brightfield set at:12-Jul-2019 05:58:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 05:58:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:30

Channel configuration set to: GFP: 12-Jul-2019 05:58:31

Channel:GFP set at:12-Jul-2019 05:58:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 05:58:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:34

Channel:Brightfield set at:12-Jul-2019 05:58:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3501. 12-Jul-2019 05:58:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:35

Channel configuration set to: GFP: 12-Jul-2019 05:58:36

Channel:GFP set at:12-Jul-2019 05:58:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3501. 12-Jul-2019 05:58:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:40

Channel:Brightfield set at:12-Jul-2019 05:58:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 05:58:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:40

Channel configuration set to: GFP: 12-Jul-2019 05:58:42

Channel:GFP set at:12-Jul-2019 05:58:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 05:58:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:46

Channel:Brightfield set at:12-Jul-2019 05:58:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 05:58:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:46

Channel configuration set to: GFP: 12-Jul-2019 05:58:47

Channel:GFP set at:12-Jul-2019 05:58:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 05:58:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:49

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:51

Channel:Brightfield set at:12-Jul-2019 05:58:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 05:58:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:51

Channel configuration set to: GFP: 12-Jul-2019 05:58:52

Channel:GFP set at:12-Jul-2019 05:58:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 05:58:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 05:58:56

Channel:Brightfield set at:12-Jul-2019 05:58:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 12-Jul-2019 05:58:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:57

Channel configuration set to: GFP: 12-Jul-2019 05:58:58

Channel:GFP set at:12-Jul-2019 05:58:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 12-Jul-2019 05:58:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:58:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:59:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 12-Jul-2019 05:59:02

Channel:Brightfield set at:12-Jul-2019 05:59:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8751. 12-Jul-2019 05:59:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:59:02

Channel configuration set to: GFP: 12-Jul-2019 05:59:03

Channel:GFP set at:12-Jul-2019 05:59:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 05:59:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:59:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 05:59:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 12-Jul-2019 05:59:07

Channel:Brightfield set at:12-Jul-2019 05:59:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 05:59:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:59:07

Channel configuration set to: GFP: 12-Jul-2019 05:59:09

Channel:GFP set at:12-Jul-2019 05:59:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 05:59:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 05:59:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:163 completed at:12-Jul-2019 05:59:11

Time since start of timelapse:48698.7034

PFS is locked

Time to next time point:202.0357



------Time point_164------

Memory available for all arrays: 61009543168

Memory used by Matlab: 2263080960

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:02:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.425

Channel configuration set to: Brightfield: 12-Jul-2019 06:02:35

Channel:Brightfield set at:12-Jul-2019 06:02:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 06:02:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:02:36

Channel configuration set to: GFP: 12-Jul-2019 06:02:37

Channel:GFP set at:12-Jul-2019 06:02:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 06:02:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:02:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:02:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.6

Channel configuration set to: Brightfield: 12-Jul-2019 06:02:40

Channel:Brightfield set at:12-Jul-2019 06:02:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 12-Jul-2019 06:02:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:02:40

Channel configuration set to: GFP: 12-Jul-2019 06:02:42

Channel:GFP set at:12-Jul-2019 06:02:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 12-Jul-2019 06:02:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:02:42

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:02:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.675

Channel configuration set to: Brightfield: 12-Jul-2019 06:02:45

Channel:Brightfield set at:12-Jul-2019 06:02:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 12-Jul-2019 06:02:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:02:45

Channel configuration set to: GFP: 12-Jul-2019 06:02:46

Channel:GFP set at:12-Jul-2019 06:02:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.2251. 12-Jul-2019 06:02:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:02:47

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:02:49

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.825

Channel configuration set to: Brightfield: 12-Jul-2019 06:02:50

Channel:Brightfield set at:12-Jul-2019 06:02:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 06:02:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:02:51

Channel configuration set to: GFP: 12-Jul-2019 06:02:52

Channel:GFP set at:12-Jul-2019 06:02:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 06:02:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:02:52

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:02:54

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.675

Channel configuration set to: Brightfield: 12-Jul-2019 06:02:55

Channel:Brightfield set at:12-Jul-2019 06:02:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 06:02:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:02:56

Channel configuration set to: GFP: 12-Jul-2019 06:02:57

Channel:GFP set at:12-Jul-2019 06:02:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 06:02:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:02:57

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:00

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.1

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:01

Channel:Brightfield set at:12-Jul-2019 06:03:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 12-Jul-2019 06:03:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:01

Channel configuration set to: GFP: 12-Jul-2019 06:03:03

Channel:GFP set at:12-Jul-2019 06:03:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 12-Jul-2019 06:03:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:03

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:05

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:07

Channel:Brightfield set at:12-Jul-2019 06:03:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 06:03:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:07

Channel configuration set to: GFP: 12-Jul-2019 06:03:08

Channel:GFP set at:12-Jul-2019 06:03:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 06:03:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:12

Channel:Brightfield set at:12-Jul-2019 06:03:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 06:03:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:12

Channel configuration set to: GFP: 12-Jul-2019 06:03:13

Channel:GFP set at:12-Jul-2019 06:03:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 06:03:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:14

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:16

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.425

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:17

Channel:Brightfield set at:12-Jul-2019 06:03:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 06:03:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:18

Channel configuration set to: GFP: 12-Jul-2019 06:03:19

Channel:GFP set at:12-Jul-2019 06:03:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 06:03:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:19

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:23

Channel:Brightfield set at:12-Jul-2019 06:03:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6501. 12-Jul-2019 06:03:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:23

Channel configuration set to: GFP: 12-Jul-2019 06:03:25

Channel:GFP set at:12-Jul-2019 06:03:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6501. 12-Jul-2019 06:03:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:28

Channel:Brightfield set at:12-Jul-2019 06:03:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3751. 12-Jul-2019 06:03:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:28

Channel configuration set to: GFP: 12-Jul-2019 06:03:30

Channel:GFP set at:12-Jul-2019 06:03:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3751. 12-Jul-2019 06:03:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:33

Channel:Brightfield set at:12-Jul-2019 06:03:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3501. 12-Jul-2019 06:03:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:34

Channel configuration set to: GFP: 12-Jul-2019 06:03:35

Channel:GFP set at:12-Jul-2019 06:03:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3501. 12-Jul-2019 06:03:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:35

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:39

Channel:Brightfield set at:12-Jul-2019 06:03:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 06:03:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:39

Channel configuration set to: GFP: 12-Jul-2019 06:03:40

Channel:GFP set at:12-Jul-2019 06:03:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 06:03:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:44

Channel:Brightfield set at:12-Jul-2019 06:03:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 06:03:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:45

Channel configuration set to: GFP: 12-Jul-2019 06:03:46

Channel:GFP set at:12-Jul-2019 06:03:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3251. 12-Jul-2019 06:03:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.4

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:49

Channel:Brightfield set at:12-Jul-2019 06:03:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 06:03:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:49

Channel configuration set to: GFP: 12-Jul-2019 06:03:51

Channel:GFP set at:12-Jul-2019 06:03:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 06:03:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:51

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:53

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:54

Channel:Brightfield set at:12-Jul-2019 06:03:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 12-Jul-2019 06:03:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:55

Channel configuration set to: GFP: 12-Jul-2019 06:03:56

Channel:GFP set at:12-Jul-2019 06:03:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 12-Jul-2019 06:03:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:03:56

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:03:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 12-Jul-2019 06:04:00

Channel:Brightfield set at:12-Jul-2019 06:04:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 06:04:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:04:01

Channel configuration set to: GFP: 12-Jul-2019 06:04:02

Channel:GFP set at:12-Jul-2019 06:04:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.8501. 12-Jul-2019 06:04:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:04:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:04:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 12-Jul-2019 06:04:05

Channel:Brightfield set at:12-Jul-2019 06:04:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 06:04:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:04:06

Channel configuration set to: GFP: 12-Jul-2019 06:04:07

Channel:GFP set at:12-Jul-2019 06:04:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 06:04:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:04:07

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:164 completed at:12-Jul-2019 06:04:10

Time since start of timelapse:48997.0883

PFS is locked

Time to next time point:203.6522



------Time point_165------

Memory available for all arrays: 61012508672

Memory used by Matlab: 2263142400

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:07:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.45

Channel configuration set to: Brightfield: 12-Jul-2019 06:07:35

Channel:Brightfield set at:12-Jul-2019 06:07:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 06:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:07:36

Channel configuration set to: GFP: 12-Jul-2019 06:07:37

Channel:GFP set at:12-Jul-2019 06:07:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 06:07:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:07:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:07:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.6

Channel configuration set to: Brightfield: 12-Jul-2019 06:07:41

Channel:Brightfield set at:12-Jul-2019 06:07:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 12-Jul-2019 06:07:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:07:41

Channel configuration set to: GFP: 12-Jul-2019 06:07:42

Channel:GFP set at:12-Jul-2019 06:07:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2501. 12-Jul-2019 06:07:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:07:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:07:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.55

Channel configuration set to: Brightfield: 12-Jul-2019 06:07:46

Channel:Brightfield set at:12-Jul-2019 06:07:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 12-Jul-2019 06:07:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:07:46

Channel configuration set to: GFP: 12-Jul-2019 06:07:48

Channel:GFP set at:12-Jul-2019 06:07:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 12-Jul-2019 06:07:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:07:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:07:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.825

Channel configuration set to: Brightfield: 12-Jul-2019 06:07:51

Channel:Brightfield set at:12-Jul-2019 06:07:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 06:07:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:07:52

Channel configuration set to: GFP: 12-Jul-2019 06:07:53

Channel:GFP set at:12-Jul-2019 06:07:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 06:07:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:07:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:07:55

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.9

Channel configuration set to: Brightfield: 12-Jul-2019 06:07:57

Channel:Brightfield set at:12-Jul-2019 06:07:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1001. 12-Jul-2019 06:07:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:07:57

Channel configuration set to: GFP: 12-Jul-2019 06:07:58

Channel:GFP set at:12-Jul-2019 06:07:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 12-Jul-2019 06:07:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:07:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:02

Channel:Brightfield set at:12-Jul-2019 06:08:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5501. 12-Jul-2019 06:08:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:02

Channel configuration set to: GFP: 12-Jul-2019 06:08:03

Channel:GFP set at:12-Jul-2019 06:08:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 12-Jul-2019 06:08:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.775

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:07

Channel:Brightfield set at:12-Jul-2019 06:08:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 06:08:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:08

Channel configuration set to: GFP: 12-Jul-2019 06:08:09

Channel:GFP set at:12-Jul-2019 06:08:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 06:08:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:09

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:11

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:13

Channel:Brightfield set at:12-Jul-2019 06:08:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 06:08:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:13

Channel configuration set to: GFP: 12-Jul-2019 06:08:15

Channel:GFP set at:12-Jul-2019 06:08:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 06:08:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:18

Channel:Brightfield set at:12-Jul-2019 06:08:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 06:08:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:18

Channel configuration set to: GFP: 12-Jul-2019 06:08:20

Channel:GFP set at:12-Jul-2019 06:08:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1001. 12-Jul-2019 06:08:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.375

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:23

Channel:Brightfield set at:12-Jul-2019 06:08:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5751. 12-Jul-2019 06:08:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:23

Channel configuration set to: GFP: 12-Jul-2019 06:08:25

Channel:GFP set at:12-Jul-2019 06:08:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5501. 12-Jul-2019 06:08:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:27

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:28

Channel:Brightfield set at:12-Jul-2019 06:08:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 06:08:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:29

Channel configuration set to: GFP: 12-Jul-2019 06:08:30

Channel:GFP set at:12-Jul-2019 06:08:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 06:08:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:30

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:32

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:34

Channel:Brightfield set at:12-Jul-2019 06:08:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 06:08:34

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:34

Channel configuration set to: GFP: 12-Jul-2019 06:08:36

Channel:GFP set at:12-Jul-2019 06:08:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 06:08:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:38

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.5

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:39

Channel:Brightfield set at:12-Jul-2019 06:08:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0001. 12-Jul-2019 06:08:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:39

Channel configuration set to: GFP: 12-Jul-2019 06:08:40

Channel:GFP set at:12-Jul-2019 06:08:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 06:08:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:41

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:43

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:44

Channel:Brightfield set at:12-Jul-2019 06:08:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 06:08:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:45

Channel configuration set to: GFP: 12-Jul-2019 06:08:46

Channel:GFP set at:12-Jul-2019 06:08:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 06:08:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:46

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:48

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:50

Channel:Brightfield set at:12-Jul-2019 06:08:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 06:08:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:50

Channel configuration set to: GFP: 12-Jul-2019 06:08:51

Channel:GFP set at:12-Jul-2019 06:08:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 06:08:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:54

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:55

Channel:Brightfield set at:12-Jul-2019 06:08:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 12-Jul-2019 06:08:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:55

Channel configuration set to: GFP: 12-Jul-2019 06:08:57

Channel:GFP set at:12-Jul-2019 06:08:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 12-Jul-2019 06:08:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:08:57

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:08:59

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.7

Channel configuration set to: Brightfield: 12-Jul-2019 06:09:01

Channel:Brightfield set at:12-Jul-2019 06:09:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 06:09:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:09:01

Channel configuration set to: GFP: 12-Jul-2019 06:09:02

Channel:GFP set at:12-Jul-2019 06:09:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.0251. 12-Jul-2019 06:09:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:09:03

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:09:04

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 12-Jul-2019 06:09:06

Channel:Brightfield set at:12-Jul-2019 06:09:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 06:09:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:09:06

Channel configuration set to: GFP: 12-Jul-2019 06:09:08

Channel:GFP set at:12-Jul-2019 06:09:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 06:09:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:09:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:165 completed at:12-Jul-2019 06:09:10

Time since start of timelapse:49297.5845

PFS is locked

Time to next time point:203.1572



------Time point_166------

Memory available for all arrays: 61007126528

Memory used by Matlab: 2263203840

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:12:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.35

Channel configuration set to: Brightfield: 12-Jul-2019 06:12:35

Channel:Brightfield set at:12-Jul-2019 06:12:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 06:12:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:12:36

Channel configuration set to: GFP: 12-Jul-2019 06:12:37

Channel:GFP set at:12-Jul-2019 06:12:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 06:12:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:12:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:12:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 12-Jul-2019 06:12:41

Channel:Brightfield set at:12-Jul-2019 06:12:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 06:12:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:12:41

Channel configuration set to: GFP: 12-Jul-2019 06:12:42

Channel:GFP set at:12-Jul-2019 06:12:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 06:12:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:12:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:12:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.325

Channel configuration set to: Brightfield: 12-Jul-2019 06:12:46

Channel:Brightfield set at:12-Jul-2019 06:12:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 06:12:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:12:47

Channel configuration set to: GFP: 12-Jul-2019 06:12:48

Channel:GFP set at:12-Jul-2019 06:12:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 06:12:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:12:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:12:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.875

Channel configuration set to: Brightfield: 12-Jul-2019 06:12:52

Channel:Brightfield set at:12-Jul-2019 06:12:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 06:12:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:12:52

Channel configuration set to: GFP: 12-Jul-2019 06:12:53

Channel:GFP set at:12-Jul-2019 06:12:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 06:12:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:12:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:12:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.825

Channel configuration set to: Brightfield: 12-Jul-2019 06:12:57

Channel:Brightfield set at:12-Jul-2019 06:12:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 06:12:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:12:58

Channel configuration set to: GFP: 12-Jul-2019 06:12:59

Channel:GFP set at:12-Jul-2019 06:12:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 06:12:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:12:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-10

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:02

Channel:Brightfield set at:12-Jul-2019 06:13:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 06:13:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:03

Channel configuration set to: GFP: 12-Jul-2019 06:13:04

Channel:GFP set at:12-Jul-2019 06:13:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 06:13:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:08

Channel:Brightfield set at:12-Jul-2019 06:13:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 06:13:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:09

Channel configuration set to: GFP: 12-Jul-2019 06:13:10

Channel:GFP set at:12-Jul-2019 06:13:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 06:13:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:14

Channel:Brightfield set at:12-Jul-2019 06:13:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 06:13:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:14

Channel configuration set to: GFP: 12-Jul-2019 06:13:16

Channel:GFP set at:12-Jul-2019 06:13:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 12-Jul-2019 06:13:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.725

PFS not locked. Waiting for lock...

PFS took 0.700000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:19

Channel:Brightfield set at:12-Jul-2019 06:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5251. 12-Jul-2019 06:13:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:19

Channel configuration set to: GFP: 12-Jul-2019 06:13:21

Channel:GFP set at:12-Jul-2019 06:13:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5501. 12-Jul-2019 06:13:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:24

Channel:Brightfield set at:12-Jul-2019 06:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8751. 12-Jul-2019 06:13:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:24

Channel configuration set to: GFP: 12-Jul-2019 06:13:25

Channel:GFP set at:12-Jul-2019 06:13:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8751. 12-Jul-2019 06:13:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.65

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:29

Channel:Brightfield set at:12-Jul-2019 06:13:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4751. 12-Jul-2019 06:13:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:30

Channel configuration set to: GFP: 12-Jul-2019 06:13:31

Channel:GFP set at:12-Jul-2019 06:13:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4751. 12-Jul-2019 06:13:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:34

Channel:Brightfield set at:12-Jul-2019 06:13:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 06:13:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:35

Channel configuration set to: GFP: 12-Jul-2019 06:13:36

Channel:GFP set at:12-Jul-2019 06:13:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 06:13:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:40

Channel:Brightfield set at:12-Jul-2019 06:13:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 06:13:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:41

Channel configuration set to: GFP: 12-Jul-2019 06:13:42

Channel:GFP set at:12-Jul-2019 06:13:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 06:13:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.275

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:46

Channel:Brightfield set at:12-Jul-2019 06:13:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 06:13:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:47

Channel configuration set to: GFP: 12-Jul-2019 06:13:48

Channel:GFP set at:12-Jul-2019 06:13:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 06:13:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:51

Channel:Brightfield set at:12-Jul-2019 06:13:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 06:13:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:51

Channel configuration set to: GFP: 12-Jul-2019 06:13:52

Channel:GFP set at:12-Jul-2019 06:13:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 06:13:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 12-Jul-2019 06:13:56

Channel:Brightfield set at:12-Jul-2019 06:13:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 06:13:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:57

Channel configuration set to: GFP: 12-Jul-2019 06:13:58

Channel:GFP set at:12-Jul-2019 06:13:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 06:13:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:13:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:14:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.625

PFS not locked. Waiting for lock...

PFS took 0.500000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:14:02

Channel:Brightfield set at:12-Jul-2019 06:14:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1751. 12-Jul-2019 06:14:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:14:02

Channel configuration set to: GFP: 12-Jul-2019 06:14:04

Channel:GFP set at:12-Jul-2019 06:14:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1751. 12-Jul-2019 06:14:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:14:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:14:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 12-Jul-2019 06:14:07

Channel:Brightfield set at:12-Jul-2019 06:14:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 06:14:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:14:07

Channel configuration set to: GFP: 12-Jul-2019 06:14:09

Channel:GFP set at:12-Jul-2019 06:14:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 06:14:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:14:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:166 completed at:12-Jul-2019 06:14:11

Time since start of timelapse:49598.639

PFS is locked

Time to next time point:202.1039



------Time point_167------

Memory available for all arrays: 61010427904

Memory used by Matlab: 2263486464

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:17:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.925

Channel configuration set to: Brightfield: 12-Jul-2019 06:17:35

Channel:Brightfield set at:12-Jul-2019 06:17:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 06:17:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:17:35

Channel configuration set to: GFP: 12-Jul-2019 06:17:36

Channel:GFP set at:12-Jul-2019 06:17:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2251. 12-Jul-2019 06:17:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:17:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:17:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.425

Channel configuration set to: Brightfield: 12-Jul-2019 06:17:41

Channel:Brightfield set at:12-Jul-2019 06:17:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 06:17:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:17:41

Channel configuration set to: GFP: 12-Jul-2019 06:17:42

Channel:GFP set at:12-Jul-2019 06:17:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 06:17:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:17:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:17:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.375

Channel configuration set to: Brightfield: 12-Jul-2019 06:17:46

Channel:Brightfield set at:12-Jul-2019 06:17:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 06:17:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:17:47

Channel configuration set to: GFP: 12-Jul-2019 06:17:48

Channel:GFP set at:12-Jul-2019 06:17:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.6251. 12-Jul-2019 06:17:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:17:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:17:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.925

Channel configuration set to: Brightfield: 12-Jul-2019 06:17:52

Channel:Brightfield set at:12-Jul-2019 06:17:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 06:17:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:17:52

Channel configuration set to: GFP: 12-Jul-2019 06:17:53

Channel:GFP set at:12-Jul-2019 06:17:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 06:17:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:17:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:17:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.825

Channel configuration set to: Brightfield: 12-Jul-2019 06:17:57

Channel:Brightfield set at:12-Jul-2019 06:17:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 06:17:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:17:58

Channel configuration set to: GFP: 12-Jul-2019 06:17:59

Channel:GFP set at:12-Jul-2019 06:17:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 06:17:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:17:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.875

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:03

Channel:Brightfield set at:12-Jul-2019 06:18:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 06:18:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:03

Channel configuration set to: GFP: 12-Jul-2019 06:18:05

Channel:GFP set at:12-Jul-2019 06:18:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 06:18:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:08

Channel:Brightfield set at:12-Jul-2019 06:18:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 06:18:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:09

Channel configuration set to: GFP: 12-Jul-2019 06:18:10

Channel:GFP set at:12-Jul-2019 06:18:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 06:18:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:13

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:14

Channel:Brightfield set at:12-Jul-2019 06:18:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 12-Jul-2019 06:18:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:14

Channel configuration set to: GFP: 12-Jul-2019 06:18:16

Channel:GFP set at:12-Jul-2019 06:18:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8501. 12-Jul-2019 06:18:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.9

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:19

Channel:Brightfield set at:12-Jul-2019 06:18:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 12-Jul-2019 06:18:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:19

Channel configuration set to: GFP: 12-Jul-2019 06:18:21

Channel:GFP set at:12-Jul-2019 06:18:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.5751. 12-Jul-2019 06:18:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:24

Channel:Brightfield set at:12-Jul-2019 06:18:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8251. 12-Jul-2019 06:18:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:24

Channel configuration set to: GFP: 12-Jul-2019 06:18:26

Channel:GFP set at:12-Jul-2019 06:18:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8251. 12-Jul-2019 06:18:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:30

Channel:Brightfield set at:12-Jul-2019 06:18:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7001. 12-Jul-2019 06:18:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:30

Channel configuration set to: GFP: 12-Jul-2019 06:18:31

Channel:GFP set at:12-Jul-2019 06:18:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7251. 12-Jul-2019 06:18:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:36

Channel:Brightfield set at:12-Jul-2019 06:18:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 06:18:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:36

Channel configuration set to: GFP: 12-Jul-2019 06:18:37

Channel:GFP set at:12-Jul-2019 06:18:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0751. 12-Jul-2019 06:18:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:38

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.525

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:41

Channel:Brightfield set at:12-Jul-2019 06:18:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 06:18:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:41

Channel configuration set to: GFP: 12-Jul-2019 06:18:42

Channel:GFP set at:12-Jul-2019 06:18:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9001. 12-Jul-2019 06:18:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:46

Channel:Brightfield set at:12-Jul-2019 06:18:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 06:18:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:47

Channel configuration set to: GFP: 12-Jul-2019 06:18:48

Channel:GFP set at:12-Jul-2019 06:18:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 06:18:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:51

Channel:Brightfield set at:12-Jul-2019 06:18:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:18:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:51

Channel configuration set to: GFP: 12-Jul-2019 06:18:53

Channel:GFP set at:12-Jul-2019 06:18:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:18:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 06:18:56

Channel:Brightfield set at:12-Jul-2019 06:18:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 12-Jul-2019 06:18:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:57

Channel configuration set to: GFP: 12-Jul-2019 06:18:58

Channel:GFP set at:12-Jul-2019 06:18:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 12-Jul-2019 06:18:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:18:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:19:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 12-Jul-2019 06:19:02

Channel:Brightfield set at:12-Jul-2019 06:19:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 12-Jul-2019 06:19:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:19:02

Channel configuration set to: GFP: 12-Jul-2019 06:19:04

Channel:GFP set at:12-Jul-2019 06:19:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 12-Jul-2019 06:19:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:19:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:19:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 12-Jul-2019 06:19:07

Channel:Brightfield set at:12-Jul-2019 06:19:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 06:19:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:19:08

Channel configuration set to: GFP: 12-Jul-2019 06:19:09

Channel:GFP set at:12-Jul-2019 06:19:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 06:19:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:19:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:167 completed at:12-Jul-2019 06:19:11

Time since start of timelapse:49898.9849

PFS is locked

Time to next time point:201.7595



------Time point_168------

Memory available for all arrays: 61010665472

Memory used by Matlab: 2264166400

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:22:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.35

Channel configuration set to: Brightfield: 12-Jul-2019 06:22:35

Channel:Brightfield set at:12-Jul-2019 06:22:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 06:22:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:22:36

Channel configuration set to: GFP: 12-Jul-2019 06:22:37

Channel:GFP set at:12-Jul-2019 06:22:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 06:22:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:22:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:22:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.425

Channel configuration set to: Brightfield: 12-Jul-2019 06:22:41

Channel:Brightfield set at:12-Jul-2019 06:22:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 06:22:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:22:41

Channel configuration set to: GFP: 12-Jul-2019 06:22:42

Channel:GFP set at:12-Jul-2019 06:22:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 06:22:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:22:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:22:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.475

Channel configuration set to: Brightfield: 12-Jul-2019 06:22:46

Channel:Brightfield set at:12-Jul-2019 06:22:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 12-Jul-2019 06:22:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:22:47

Channel configuration set to: GFP: 12-Jul-2019 06:22:48

Channel:GFP set at:12-Jul-2019 06:22:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5001. 12-Jul-2019 06:22:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:22:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:22:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.85

Channel configuration set to: Brightfield: 12-Jul-2019 06:22:52

Channel:Brightfield set at:12-Jul-2019 06:22:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 06:22:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:22:52

Channel configuration set to: GFP: 12-Jul-2019 06:22:53

Channel:GFP set at:12-Jul-2019 06:22:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 06:22:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:22:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:22:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.825

Channel configuration set to: Brightfield: 12-Jul-2019 06:22:57

Channel:Brightfield set at:12-Jul-2019 06:22:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 06:22:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:22:58

Channel configuration set to: GFP: 12-Jul-2019 06:22:59

Channel:GFP set at:12-Jul-2019 06:22:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1501. 12-Jul-2019 06:22:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:22:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.3

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:02

Channel:Brightfield set at:12-Jul-2019 06:23:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 06:23:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:03

Channel configuration set to: GFP: 12-Jul-2019 06:23:04

Channel:GFP set at:12-Jul-2019 06:23:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3751. 12-Jul-2019 06:23:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:08

Channel:Brightfield set at:12-Jul-2019 06:23:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:23:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:08

Channel configuration set to: GFP: 12-Jul-2019 06:23:09

Channel:GFP set at:12-Jul-2019 06:23:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:23:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:13

Channel:Brightfield set at:12-Jul-2019 06:23:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8751. 12-Jul-2019 06:23:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:14

Channel configuration set to: GFP: 12-Jul-2019 06:23:15

Channel:GFP set at:12-Jul-2019 06:23:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 06:23:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.5

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:18

Channel:Brightfield set at:12-Jul-2019 06:23:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 06:23:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:19

Channel configuration set to: GFP: 12-Jul-2019 06:23:20

Channel:GFP set at:12-Jul-2019 06:23:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 06:23:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:22

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.35

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:23

Channel:Brightfield set at:12-Jul-2019 06:23:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5751. 12-Jul-2019 06:23:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:24

Channel configuration set to: GFP: 12-Jul-2019 06:23:25

Channel:GFP set at:12-Jul-2019 06:23:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5751. 12-Jul-2019 06:23:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:25

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:29

Channel:Brightfield set at:12-Jul-2019 06:23:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3251. 12-Jul-2019 06:23:29

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:29

Channel configuration set to: GFP: 12-Jul-2019 06:23:30

Channel:GFP set at:12-Jul-2019 06:23:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3251. 12-Jul-2019 06:23:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:31

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:33

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:34

Channel:Brightfield set at:12-Jul-2019 06:23:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 06:23:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:35

Channel configuration set to: GFP: 12-Jul-2019 06:23:36

Channel:GFP set at:12-Jul-2019 06:23:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9501. 12-Jul-2019 06:23:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:36

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.575

PFS not locked. Waiting for lock...

PFS took 0.050000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:40

Channel:Brightfield set at:12-Jul-2019 06:23:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 06:23:40

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:40

Channel configuration set to: GFP: 12-Jul-2019 06:23:41

Channel:GFP set at:12-Jul-2019 06:23:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 06:23:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:44

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:45

Channel:Brightfield set at:12-Jul-2019 06:23:45

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 06:23:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:46

Channel configuration set to: GFP: 12-Jul-2019 06:23:47

Channel:GFP set at:12-Jul-2019 06:23:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 06:23:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:47

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:50

Channel:Brightfield set at:12-Jul-2019 06:23:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 06:23:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:51

Channel configuration set to: GFP: 12-Jul-2019 06:23:52

Channel:GFP set at:12-Jul-2019 06:23:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9501. 12-Jul-2019 06:23:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:52

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 12-Jul-2019 06:23:56

Channel:Brightfield set at:12-Jul-2019 06:23:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 12-Jul-2019 06:23:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:56

Channel configuration set to: GFP: 12-Jul-2019 06:23:57

Channel:GFP set at:12-Jul-2019 06:23:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 12-Jul-2019 06:23:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:23:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:24:00

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-7.275

Channel configuration set to: Brightfield: 12-Jul-2019 06:24:00

Channel:Brightfield set at:12-Jul-2019 06:24:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 12-Jul-2019 06:24:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:24:01

Channel configuration set to: GFP: 12-Jul-2019 06:24:02

Channel:GFP set at:12-Jul-2019 06:24:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 12-Jul-2019 06:24:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:24:02

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:24:05

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 12-Jul-2019 06:24:06

Channel:Brightfield set at:12-Jul-2019 06:24:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 06:24:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:24:06

Channel configuration set to: GFP: 12-Jul-2019 06:24:08

Channel:GFP set at:12-Jul-2019 06:24:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 06:24:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:24:08

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:168 completed at:12-Jul-2019 06:24:10

Time since start of timelapse:50197.5762

PFS is locked

Time to next time point:203.1699



------Time point_169------

Memory available for all arrays: 61020065792

Memory used by Matlab: 2264428544

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:27:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.475

Channel configuration set to: Brightfield: 12-Jul-2019 06:27:35

Channel:Brightfield set at:12-Jul-2019 06:27:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 06:27:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:27:35

Channel configuration set to: GFP: 12-Jul-2019 06:27:37

Channel:GFP set at:12-Jul-2019 06:27:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 06:27:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:27:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:27:39

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.625

Channel configuration set to: Brightfield: 12-Jul-2019 06:27:41

Channel:Brightfield set at:12-Jul-2019 06:27:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 12-Jul-2019 06:27:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:27:41

Channel configuration set to: GFP: 12-Jul-2019 06:27:42

Channel:GFP set at:12-Jul-2019 06:27:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2251. 12-Jul-2019 06:27:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:27:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:27:44

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.525

Channel configuration set to: Brightfield: 12-Jul-2019 06:27:46

Channel:Brightfield set at:12-Jul-2019 06:27:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 06:27:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:27:46

Channel configuration set to: GFP: 12-Jul-2019 06:27:48

Channel:GFP set at:12-Jul-2019 06:27:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4501. 12-Jul-2019 06:27:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:27:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:27:50

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.9

Channel configuration set to: Brightfield: 12-Jul-2019 06:27:51

Channel:Brightfield set at:12-Jul-2019 06:27:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 06:27:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:27:52

Channel configuration set to: GFP: 12-Jul-2019 06:27:53

Channel:GFP set at:12-Jul-2019 06:27:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 06:27:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:27:53

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:27:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.875

Channel configuration set to: Brightfield: 12-Jul-2019 06:27:57

Channel:Brightfield set at:12-Jul-2019 06:27:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1001. 12-Jul-2019 06:27:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:27:57

Channel configuration set to: GFP: 12-Jul-2019 06:27:59

Channel:GFP set at:12-Jul-2019 06:27:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1001. 12-Jul-2019 06:27:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:27:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:01

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.2

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:02

Channel:Brightfield set at:12-Jul-2019 06:28:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 12-Jul-2019 06:28:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:02

Channel configuration set to: GFP: 12-Jul-2019 06:28:04

Channel:GFP set at:12-Jul-2019 06:28:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.6001. 12-Jul-2019 06:28:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:08

Channel:Brightfield set at:12-Jul-2019 06:28:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 06:28:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:08

Channel configuration set to: GFP: 12-Jul-2019 06:28:09

Channel:GFP set at:12-Jul-2019 06:28:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:28:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:13

Channel:Brightfield set at:12-Jul-2019 06:28:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 06:28:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:14

Channel configuration set to: GFP: 12-Jul-2019 06:28:15

Channel:GFP set at:12-Jul-2019 06:28:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 06:28:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:19

Channel:Brightfield set at:12-Jul-2019 06:28:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 12-Jul-2019 06:28:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:19

Channel configuration set to: GFP: 12-Jul-2019 06:28:21

Channel:GFP set at:12-Jul-2019 06:28:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.4751. 12-Jul-2019 06:28:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:24

Channel:Brightfield set at:12-Jul-2019 06:28:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 12-Jul-2019 06:28:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:24

Channel configuration set to: GFP: 12-Jul-2019 06:28:26

Channel:GFP set at:12-Jul-2019 06:28:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 12-Jul-2019 06:28:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.5

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:30

Channel:Brightfield set at:12-Jul-2019 06:28:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6001. 12-Jul-2019 06:28:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:30

Channel configuration set to: GFP: 12-Jul-2019 06:28:31

Channel:GFP set at:12-Jul-2019 06:28:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6001. 12-Jul-2019 06:28:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:35

Channel:Brightfield set at:12-Jul-2019 06:28:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3001. 12-Jul-2019 06:28:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:35

Channel configuration set to: GFP: 12-Jul-2019 06:28:37

Channel:GFP set at:12-Jul-2019 06:28:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3001. 12-Jul-2019 06:28:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:41

Channel:Brightfield set at:12-Jul-2019 06:28:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 06:28:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:41

Channel configuration set to: GFP: 12-Jul-2019 06:28:43

Channel:GFP set at:12-Jul-2019 06:28:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 06:28:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:47

Channel:Brightfield set at:12-Jul-2019 06:28:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 06:28:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:47

Channel configuration set to: GFP: 12-Jul-2019 06:28:49

Channel:GFP set at:12-Jul-2019 06:28:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 06:28:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:49

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:51

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.375

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:52

Channel:Brightfield set at:12-Jul-2019 06:28:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 06:28:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:52

Channel configuration set to: GFP: 12-Jul-2019 06:28:54

Channel:GFP set at:12-Jul-2019 06:28:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 06:28:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:54

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 06:28:57

Channel:Brightfield set at:12-Jul-2019 06:28:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3001. 12-Jul-2019 06:28:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:58

Channel configuration set to: GFP: 12-Jul-2019 06:28:59

Channel:GFP set at:12-Jul-2019 06:28:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2751. 12-Jul-2019 06:28:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:28:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:29:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-7.15

PFS not locked. Waiting for lock...

PFS took 0.400000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:29:02

Channel:Brightfield set at:12-Jul-2019 06:29:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1251. 12-Jul-2019 06:29:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:29:03

Channel configuration set to: GFP: 12-Jul-2019 06:29:04

Channel:GFP set at:12-Jul-2019 06:29:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.1501. 12-Jul-2019 06:29:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:29:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:29:07

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 12-Jul-2019 06:29:08

Channel:Brightfield set at:12-Jul-2019 06:29:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 06:29:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:29:08

Channel configuration set to: GFP: 12-Jul-2019 06:29:10

Channel:GFP set at:12-Jul-2019 06:29:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 06:29:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:29:10

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:169 completed at:12-Jul-2019 06:29:12

Time since start of timelapse:50499.7697

PFS is locked

Time to next time point:200.9778



------Time point_170------

Memory available for all arrays: 61019262976

Memory used by Matlab: 2264428544

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:32:33

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.275

Channel configuration set to: Brightfield: 12-Jul-2019 06:32:35

Channel:Brightfield set at:12-Jul-2019 06:32:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 06:32:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:32:36

Channel configuration set to: GFP: 12-Jul-2019 06:32:37

Channel:GFP set at:12-Jul-2019 06:32:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 06:32:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:32:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:32:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-11.8

Channel configuration set to: Brightfield: 12-Jul-2019 06:32:41

Channel:Brightfield set at:12-Jul-2019 06:32:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 12-Jul-2019 06:32:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:32:41

Channel configuration set to: GFP: 12-Jul-2019 06:32:42

Channel:GFP set at:12-Jul-2019 06:32:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4251. 12-Jul-2019 06:32:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:32:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:32:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.225

Channel configuration set to: Brightfield: 12-Jul-2019 06:32:46

Channel:Brightfield set at:12-Jul-2019 06:32:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 12-Jul-2019 06:32:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:32:47

Channel configuration set to: GFP: 12-Jul-2019 06:32:48

Channel:GFP set at:12-Jul-2019 06:32:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 12-Jul-2019 06:32:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:32:48

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:32:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.85

Channel configuration set to: Brightfield: 12-Jul-2019 06:32:52

Channel:Brightfield set at:12-Jul-2019 06:32:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 06:32:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:32:52

Channel configuration set to: GFP: 12-Jul-2019 06:32:53

Channel:GFP set at:12-Jul-2019 06:32:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 06:32:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:32:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:32:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.775

Channel configuration set to: Brightfield: 12-Jul-2019 06:32:57

Channel:Brightfield set at:12-Jul-2019 06:32:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2751. 12-Jul-2019 06:32:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:32:58

Channel configuration set to: GFP: 12-Jul-2019 06:32:59

Channel:GFP set at:12-Jul-2019 06:32:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2501. 12-Jul-2019 06:32:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:00

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:03

Channel:Brightfield set at:12-Jul-2019 06:33:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 06:33:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:03

Channel configuration set to: GFP: 12-Jul-2019 06:33:04

Channel:GFP set at:12-Jul-2019 06:33:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 12-Jul-2019 06:33:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.45

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:08

Channel:Brightfield set at:12-Jul-2019 06:33:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 06:33:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:09

Channel configuration set to: GFP: 12-Jul-2019 06:33:10

Channel:GFP set at:12-Jul-2019 06:33:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 06:33:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:14

Channel:Brightfield set at:12-Jul-2019 06:33:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9251. 12-Jul-2019 06:33:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:14

Channel configuration set to: GFP: 12-Jul-2019 06:33:15

Channel:GFP set at:12-Jul-2019 06:33:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9251. 12-Jul-2019 06:33:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.35

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:19

Channel:Brightfield set at:12-Jul-2019 06:33:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 06:33:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:20

Channel configuration set to: GFP: 12-Jul-2019 06:33:21

Channel:GFP set at:12-Jul-2019 06:33:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7251. 12-Jul-2019 06:33:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.075

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:24

Channel:Brightfield set at:12-Jul-2019 06:33:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8751. 12-Jul-2019 06:33:25

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:25

Channel configuration set to: GFP: 12-Jul-2019 06:33:26

Channel:GFP set at:12-Jul-2019 06:33:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8751. 12-Jul-2019 06:33:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:29

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.65

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:30

Channel:Brightfield set at:12-Jul-2019 06:33:30

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5001. 12-Jul-2019 06:33:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:30

Channel configuration set to: GFP: 12-Jul-2019 06:33:31

Channel:GFP set at:12-Jul-2019 06:33:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5001. 12-Jul-2019 06:33:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:35

Channel:Brightfield set at:12-Jul-2019 06:33:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 06:33:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:36

Channel configuration set to: GFP: 12-Jul-2019 06:33:37

Channel:GFP set at:12-Jul-2019 06:33:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 06:33:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:41

Channel:Brightfield set at:12-Jul-2019 06:33:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 06:33:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:41

Channel configuration set to: GFP: 12-Jul-2019 06:33:42

Channel:GFP set at:12-Jul-2019 06:33:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 06:33:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:46

Channel:Brightfield set at:12-Jul-2019 06:33:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 06:33:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:47

Channel configuration set to: GFP: 12-Jul-2019 06:33:48

Channel:GFP set at:12-Jul-2019 06:33:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 06:33:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:49

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:51

Channel:Brightfield set at:12-Jul-2019 06:33:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 06:33:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:52

Channel configuration set to: GFP: 12-Jul-2019 06:33:53

Channel:GFP set at:12-Jul-2019 06:33:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 06:33:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:56

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 12-Jul-2019 06:33:57

Channel:Brightfield set at:12-Jul-2019 06:33:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 06:33:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:57

Channel configuration set to: GFP: 12-Jul-2019 06:33:58

Channel:GFP set at:12-Jul-2019 06:33:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 06:33:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:33:59

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:34:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.425

Channel configuration set to: Brightfield: 12-Jul-2019 06:34:03

Channel:Brightfield set at:12-Jul-2019 06:34:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3001. 12-Jul-2019 06:34:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:34:03

Channel configuration set to: GFP: 12-Jul-2019 06:34:04

Channel:GFP set at:12-Jul-2019 06:34:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3251. 12-Jul-2019 06:34:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:34:05

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:34:07

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 12-Jul-2019 06:34:08

Channel:Brightfield set at:12-Jul-2019 06:34:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 06:34:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:34:09

Channel configuration set to: GFP: 12-Jul-2019 06:34:10

Channel:GFP set at:12-Jul-2019 06:34:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 06:34:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:34:10

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:170 completed at:12-Jul-2019 06:34:12

Time since start of timelapse:50799.9222

PFS is locked

Time to next time point:200.8269



------Time point_171------

Memory available for all arrays: 61033840640

Memory used by Matlab: 2264428544

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:37:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.175

Channel configuration set to: Brightfield: 12-Jul-2019 06:37:35

Channel:Brightfield set at:12-Jul-2019 06:37:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:37:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:37:36

Channel configuration set to: GFP: 12-Jul-2019 06:37:37

Channel:GFP set at:12-Jul-2019 06:37:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:37:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:37:37

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:37:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 12-Jul-2019 06:37:41

Channel:Brightfield set at:12-Jul-2019 06:37:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 06:37:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:37:41

Channel configuration set to: GFP: 12-Jul-2019 06:37:43

Channel:GFP set at:12-Jul-2019 06:37:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 06:37:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:37:43

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:37:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.225

Channel configuration set to: Brightfield: 12-Jul-2019 06:37:47

Channel:Brightfield set at:12-Jul-2019 06:37:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 12-Jul-2019 06:37:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:37:47

Channel configuration set to: GFP: 12-Jul-2019 06:37:48

Channel:GFP set at:12-Jul-2019 06:37:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 12-Jul-2019 06:37:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:37:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:37:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.775

Channel configuration set to: Brightfield: 12-Jul-2019 06:37:52

Channel:Brightfield set at:12-Jul-2019 06:37:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9001. 12-Jul-2019 06:37:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:37:52

Channel configuration set to: GFP: 12-Jul-2019 06:37:54

Channel:GFP set at:12-Jul-2019 06:37:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9251. 12-Jul-2019 06:37:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:37:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:37:56

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.3

Channel configuration set to: Brightfield: 12-Jul-2019 06:37:57

Channel:Brightfield set at:12-Jul-2019 06:37:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 06:37:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:37:58

Channel configuration set to: GFP: 12-Jul-2019 06:37:59

Channel:GFP set at:12-Jul-2019 06:37:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 06:37:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:37:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.15

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:02

Channel:Brightfield set at:12-Jul-2019 06:38:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 06:38:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:03

Channel configuration set to: GFP: 12-Jul-2019 06:38:04

Channel:GFP set at:12-Jul-2019 06:38:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 06:38:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:04

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:06

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:08

Channel:Brightfield set at:12-Jul-2019 06:38:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 06:38:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:08

Channel configuration set to: GFP: 12-Jul-2019 06:38:10

Channel:GFP set at:12-Jul-2019 06:38:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 06:38:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:13

Channel:Brightfield set at:12-Jul-2019 06:38:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:38:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:13

Channel configuration set to: GFP: 12-Jul-2019 06:38:15

Channel:GFP set at:12-Jul-2019 06:38:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:38:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:17

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.3

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:18

Channel:Brightfield set at:12-Jul-2019 06:38:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 06:38:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:19

Channel configuration set to: GFP: 12-Jul-2019 06:38:20

Channel:GFP set at:12-Jul-2019 06:38:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 06:38:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:20

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:24

Channel:Brightfield set at:12-Jul-2019 06:38:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 06:38:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:24

Channel configuration set to: GFP: 12-Jul-2019 06:38:26

Channel:GFP set at:12-Jul-2019 06:38:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8001. 12-Jul-2019 06:38:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:29

Channel:Brightfield set at:12-Jul-2019 06:38:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4751. 12-Jul-2019 06:38:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:30

Channel configuration set to: GFP: 12-Jul-2019 06:38:31

Channel:GFP set at:12-Jul-2019 06:38:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4501. 12-Jul-2019 06:38:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:34

Channel:Brightfield set at:12-Jul-2019 06:38:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5001. 12-Jul-2019 06:38:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:35

Channel configuration set to: GFP: 12-Jul-2019 06:38:36

Channel:GFP set at:12-Jul-2019 06:38:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5001. 12-Jul-2019 06:38:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:40

Channel:Brightfield set at:12-Jul-2019 06:38:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:38:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:41

Channel configuration set to: GFP: 12-Jul-2019 06:38:42

Channel:GFP set at:12-Jul-2019 06:38:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:38:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:42

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.125

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:46

Channel:Brightfield set at:12-Jul-2019 06:38:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 06:38:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:47

Channel configuration set to: GFP: 12-Jul-2019 06:38:48

Channel:GFP set at:12-Jul-2019 06:38:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 06:38:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:50

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.8

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:51

Channel:Brightfield set at:12-Jul-2019 06:38:51

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 06:38:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:52

Channel configuration set to: GFP: 12-Jul-2019 06:38:53

Channel:GFP set at:12-Jul-2019 06:38:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 06:38:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:53

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:55

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 12-Jul-2019 06:38:56

Channel:Brightfield set at:12-Jul-2019 06:38:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 06:38:57

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:57

Channel configuration set to: GFP: 12-Jul-2019 06:38:58

Channel:GFP set at:12-Jul-2019 06:38:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 06:38:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:38:58

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:39:01

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.4

Channel configuration set to: Brightfield: 12-Jul-2019 06:39:02

Channel:Brightfield set at:12-Jul-2019 06:39:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.3751. 12-Jul-2019 06:39:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:39:02

Channel configuration set to: GFP: 12-Jul-2019 06:39:04

Channel:GFP set at:12-Jul-2019 06:39:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4001. 12-Jul-2019 06:39:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:39:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:39:06

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 12-Jul-2019 06:39:07

Channel:Brightfield set at:12-Jul-2019 06:39:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 06:39:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:39:07

Channel configuration set to: GFP: 12-Jul-2019 06:39:09

Channel:GFP set at:12-Jul-2019 06:39:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 06:39:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:39:09

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:171 completed at:12-Jul-2019 06:39:11

Time since start of timelapse:51098.1245

PFS is locked

Time to next time point:202.6261



------Time point_172------

Memory available for all arrays: 61036113920

Memory used by Matlab: 2264428544

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:42:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.225

Channel configuration set to: Brightfield: 12-Jul-2019 06:42:36

Channel:Brightfield set at:12-Jul-2019 06:42:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 12-Jul-2019 06:42:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:42:36

Channel configuration set to: GFP: 12-Jul-2019 06:42:38

Channel:GFP set at:12-Jul-2019 06:42:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:42:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:42:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:42:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 12-Jul-2019 06:42:41

Channel:Brightfield set at:12-Jul-2019 06:42:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5251. 12-Jul-2019 06:42:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:42:42

Channel configuration set to: GFP: 12-Jul-2019 06:42:43

Channel:GFP set at:12-Jul-2019 06:42:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 06:42:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:42:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:42:46

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-8.4

Channel configuration set to: Brightfield: 12-Jul-2019 06:42:47

Channel:Brightfield set at:12-Jul-2019 06:42:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.4751. 12-Jul-2019 06:42:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:42:47

Channel configuration set to: GFP: 12-Jul-2019 06:42:48

Channel:GFP set at:12-Jul-2019 06:42:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5001. 12-Jul-2019 06:42:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:42:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:42:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.625

Channel configuration set to: Brightfield: 12-Jul-2019 06:42:52

Channel:Brightfield set at:12-Jul-2019 06:42:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 06:42:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:42:53

Channel configuration set to: GFP: 12-Jul-2019 06:42:54

Channel:GFP set at:12-Jul-2019 06:42:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 06:42:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:42:55

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:42:57

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 12-Jul-2019 06:42:58

Channel:Brightfield set at:12-Jul-2019 06:42:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 06:42:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:42:58

Channel configuration set to: GFP: 12-Jul-2019 06:42:59

Channel:GFP set at:12-Jul-2019 06:42:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 06:43:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:00

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.125

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:03

Channel:Brightfield set at:12-Jul-2019 06:43:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 06:43:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:04

Channel configuration set to: GFP: 12-Jul-2019 06:43:05

Channel:GFP set at:12-Jul-2019 06:43:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 06:43:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:08

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:09

Channel:Brightfield set at:12-Jul-2019 06:43:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 06:43:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:09

Channel configuration set to: GFP: 12-Jul-2019 06:43:11

Channel:GFP set at:12-Jul-2019 06:43:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 06:43:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:11

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:13

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.75

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:14

Channel:Brightfield set at:12-Jul-2019 06:43:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 06:43:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:15

Channel configuration set to: GFP: 12-Jul-2019 06:43:16

Channel:GFP set at:12-Jul-2019 06:43:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 06:43:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:19

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.3

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:20

Channel:Brightfield set at:12-Jul-2019 06:43:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1751. 12-Jul-2019 06:43:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:20

Channel configuration set to: GFP: 12-Jul-2019 06:43:21

Channel:GFP set at:12-Jul-2019 06:43:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 06:43:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:22

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:24

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.15

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:25

Channel:Brightfield set at:12-Jul-2019 06:43:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 06:43:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:26

Channel configuration set to: GFP: 12-Jul-2019 06:43:27

Channel:GFP set at:12-Jul-2019 06:43:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 06:43:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:27

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:30

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.725

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:31

Channel:Brightfield set at:12-Jul-2019 06:43:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4251. 12-Jul-2019 06:43:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:31

Channel configuration set to: GFP: 12-Jul-2019 06:43:32

Channel:GFP set at:12-Jul-2019 06:43:32

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 06:43:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:33

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:35

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:36

Channel:Brightfield set at:12-Jul-2019 06:43:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3251. 12-Jul-2019 06:43:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:36

Channel configuration set to: GFP: 12-Jul-2019 06:43:37

Channel:GFP set at:12-Jul-2019 06:43:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3001. 12-Jul-2019 06:43:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:38

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:40

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:42

Channel:Brightfield set at:12-Jul-2019 06:43:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 06:43:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:42

Channel configuration set to: GFP: 12-Jul-2019 06:43:43

Channel:GFP set at:12-Jul-2019 06:43:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0751. 12-Jul-2019 06:43:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:44

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:46

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:47

Channel:Brightfield set at:12-Jul-2019 06:43:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 06:43:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:48

Channel configuration set to: GFP: 12-Jul-2019 06:43:49

Channel:GFP set at:12-Jul-2019 06:43:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 06:43:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:50

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:51

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.125

PFS not locked. Waiting for lock...

PFS took 0.700000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:53

Channel:Brightfield set at:12-Jul-2019 06:43:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 06:43:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:53

Channel configuration set to: GFP: 12-Jul-2019 06:43:54

Channel:GFP set at:12-Jul-2019 06:43:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 06:43:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:55

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:57

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 12-Jul-2019 06:43:58

Channel:Brightfield set at:12-Jul-2019 06:43:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 06:43:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:43:59

Channel configuration set to: GFP: 12-Jul-2019 06:44:00

Channel:GFP set at:12-Jul-2019 06:44:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 06:44:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:44:00

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:44:03

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 06:44:03

Channel:Brightfield set at:12-Jul-2019 06:44:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 12-Jul-2019 06:44:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:44:04

Channel configuration set to: GFP: 12-Jul-2019 06:44:05

Channel:GFP set at:12-Jul-2019 06:44:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.2001. 12-Jul-2019 06:44:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:44:05

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:44:07

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 12-Jul-2019 06:44:09

Channel:Brightfield set at:12-Jul-2019 06:44:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 06:44:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:44:09

Channel configuration set to: GFP: 12-Jul-2019 06:44:10

Channel:GFP set at:12-Jul-2019 06:44:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 06:44:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:44:11

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:172 completed at:12-Jul-2019 06:44:13

Time since start of timelapse:51399.6112

PFS is locked

Time to next time point:201.141



------Time point_173------

Memory available for all arrays: 61030862848

Memory used by Matlab: 2264428544

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:47:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.325

Channel configuration set to: Brightfield: 12-Jul-2019 06:47:36

Channel:Brightfield set at:12-Jul-2019 06:47:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 06:47:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:47:37

Channel configuration set to: GFP: 12-Jul-2019 06:47:38

Channel:GFP set at:12-Jul-2019 06:47:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0001. 12-Jul-2019 06:47:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:47:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:47:41

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.4

Channel configuration set to: Brightfield: 12-Jul-2019 06:47:42

Channel:Brightfield set at:12-Jul-2019 06:47:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4751. 12-Jul-2019 06:47:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:47:42

Channel configuration set to: GFP: 12-Jul-2019 06:47:43

Channel:GFP set at:12-Jul-2019 06:47:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4751. 12-Jul-2019 06:47:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:47:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:47:46

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.75

PFS not locked. Waiting for lock...

PFS took 0.700000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:47:48

Channel:Brightfield set at:12-Jul-2019 06:47:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 06:47:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:47:48

Channel configuration set to: GFP: 12-Jul-2019 06:47:49

Channel:GFP set at:12-Jul-2019 06:47:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.5251. 12-Jul-2019 06:47:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:47:50

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:47:52

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.8

Channel configuration set to: Brightfield: 12-Jul-2019 06:47:53

Channel:Brightfield set at:12-Jul-2019 06:47:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8251. 12-Jul-2019 06:47:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:47:53

Channel configuration set to: GFP: 12-Jul-2019 06:47:55

Channel:GFP set at:12-Jul-2019 06:47:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8001. 12-Jul-2019 06:47:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:47:55

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:47:57

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.975

Channel configuration set to: Brightfield: 12-Jul-2019 06:47:59

Channel:Brightfield set at:12-Jul-2019 06:47:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 06:47:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:47:59

Channel configuration set to: GFP: 12-Jul-2019 06:48:00

Channel:GFP set at:12-Jul-2019 06:48:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 06:48:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:01

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:03

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.225

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:04

Channel:Brightfield set at:12-Jul-2019 06:48:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 12-Jul-2019 06:48:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:04

Channel configuration set to: GFP: 12-Jul-2019 06:48:05

Channel:GFP set at:12-Jul-2019 06:48:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5751. 12-Jul-2019 06:48:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:06

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:08

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:09

Channel:Brightfield set at:12-Jul-2019 06:48:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 06:48:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:10

Channel configuration set to: GFP: 12-Jul-2019 06:48:11

Channel:GFP set at:12-Jul-2019 06:48:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3001. 12-Jul-2019 06:48:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:11

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:14

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.6

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:14

Channel:Brightfield set at:12-Jul-2019 06:48:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:48:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:15

Channel configuration set to: GFP: 12-Jul-2019 06:48:16

Channel:GFP set at:12-Jul-2019 06:48:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:48:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:19

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:20

Channel:Brightfield set at:12-Jul-2019 06:48:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 06:48:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:20

Channel configuration set to: GFP: 12-Jul-2019 06:48:22

Channel:GFP set at:12-Jul-2019 06:48:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0251. 12-Jul-2019 06:48:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:22

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:25

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-6.55

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:25

Channel:Brightfield set at:12-Jul-2019 06:48:25

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5251. 12-Jul-2019 06:48:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:26

Channel configuration set to: GFP: 12-Jul-2019 06:48:27

Channel:GFP set at:12-Jul-2019 06:48:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.5001. 12-Jul-2019 06:48:27

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:27

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:30

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:31

Channel:Brightfield set at:12-Jul-2019 06:48:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3001. 12-Jul-2019 06:48:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:31

Channel configuration set to: GFP: 12-Jul-2019 06:48:33

Channel:GFP set at:12-Jul-2019 06:48:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3001. 12-Jul-2019 06:48:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:33

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:35

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-7.05

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:36

Channel:Brightfield set at:12-Jul-2019 06:48:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4251. 12-Jul-2019 06:48:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:36

Channel configuration set to: GFP: 12-Jul-2019 06:48:38

Channel:GFP set at:12-Jul-2019 06:48:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4251. 12-Jul-2019 06:48:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:38

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:40

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:42

Channel:Brightfield set at:12-Jul-2019 06:48:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 06:48:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:42

Channel configuration set to: GFP: 12-Jul-2019 06:48:44

Channel:GFP set at:12-Jul-2019 06:48:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0501. 12-Jul-2019 06:48:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:44

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:46

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:48

Channel:Brightfield set at:12-Jul-2019 06:48:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 06:48:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:48

Channel configuration set to: GFP: 12-Jul-2019 06:48:49

Channel:GFP set at:12-Jul-2019 06:48:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6501. 12-Jul-2019 06:48:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:50

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:51

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.975

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:53

Channel:Brightfield set at:12-Jul-2019 06:48:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 06:48:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:53

Channel configuration set to: GFP: 12-Jul-2019 06:48:54

Channel:GFP set at:12-Jul-2019 06:48:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 06:48:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:55

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:57

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.95

Channel configuration set to: Brightfield: 12-Jul-2019 06:48:58

Channel:Brightfield set at:12-Jul-2019 06:48:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 06:48:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:48:58

Channel configuration set to: GFP: 12-Jul-2019 06:49:00

Channel:GFP set at:12-Jul-2019 06:49:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 06:49:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:49:00

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:49:03

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.65

Channel configuration set to: Brightfield: 12-Jul-2019 06:49:03

Channel:Brightfield set at:12-Jul-2019 06:49:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 12-Jul-2019 06:49:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:49:04

Channel configuration set to: GFP: 12-Jul-2019 06:49:05

Channel:GFP set at:12-Jul-2019 06:49:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.1001. 12-Jul-2019 06:49:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:49:05

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:49:08

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.95

Channel configuration set to: Brightfield: 12-Jul-2019 06:49:09

Channel:Brightfield set at:12-Jul-2019 06:49:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 06:49:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:49:09

Channel configuration set to: GFP: 12-Jul-2019 06:49:11

Channel:GFP set at:12-Jul-2019 06:49:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 06:49:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:49:11

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:173 completed at:12-Jul-2019 06:49:13

Time since start of timelapse:51699.7522

PFS is locked

Time to next time point:201.0013



------Time point_174------

Memory available for all arrays: 61033676800

Memory used by Matlab: 2264821760

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:52:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.45

Channel configuration set to: Brightfield: 12-Jul-2019 06:52:36

Channel:Brightfield set at:12-Jul-2019 06:52:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8501. 12-Jul-2019 06:52:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:52:37

Channel configuration set to: GFP: 12-Jul-2019 06:52:38

Channel:GFP set at:12-Jul-2019 06:52:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.8751. 12-Jul-2019 06:52:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:52:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:52:41

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.55

Channel configuration set to: Brightfield: 12-Jul-2019 06:52:42

Channel:Brightfield set at:12-Jul-2019 06:52:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3251. 12-Jul-2019 06:52:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:52:42

Channel configuration set to: GFP: 12-Jul-2019 06:52:44

Channel:GFP set at:12-Jul-2019 06:52:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3251. 12-Jul-2019 06:52:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:52:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:52:46

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.5

Channel configuration set to: Brightfield: 12-Jul-2019 06:52:47

Channel:Brightfield set at:12-Jul-2019 06:52:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 12-Jul-2019 06:52:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:52:48

Channel configuration set to: GFP: 12-Jul-2019 06:52:49

Channel:GFP set at:12-Jul-2019 06:52:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4751. 12-Jul-2019 06:52:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:52:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:52:52

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.85

Channel configuration set to: Brightfield: 12-Jul-2019 06:52:53

Channel:Brightfield set at:12-Jul-2019 06:52:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 06:52:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:52:53

Channel configuration set to: GFP: 12-Jul-2019 06:52:54

Channel:GFP set at:12-Jul-2019 06:52:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7751. 12-Jul-2019 06:52:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:52:55

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:52:57

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-10

Channel configuration set to: Brightfield: 12-Jul-2019 06:52:58

Channel:Brightfield set at:12-Jul-2019 06:52:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 06:52:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:52:59

Channel configuration set to: GFP: 12-Jul-2019 06:53:00

Channel:GFP set at:12-Jul-2019 06:53:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 06:53:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:00

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-8.95

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:04

Channel:Brightfield set at:12-Jul-2019 06:53:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 06:53:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:04

Channel configuration set to: GFP: 12-Jul-2019 06:53:06

Channel:GFP set at:12-Jul-2019 06:53:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 12-Jul-2019 06:53:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:06

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:08

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.575

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:10

Channel:Brightfield set at:12-Jul-2019 06:53:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 06:53:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:10

Channel configuration set to: GFP: 12-Jul-2019 06:53:11

Channel:GFP set at:12-Jul-2019 06:53:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2751. 12-Jul-2019 06:53:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:12

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:14

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-8.1

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:15

Channel:Brightfield set at:12-Jul-2019 06:53:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8251. 12-Jul-2019 06:53:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:16

Channel configuration set to: GFP: 12-Jul-2019 06:53:17

Channel:GFP set at:12-Jul-2019 06:53:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 06:53:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:17

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:20

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-8.475

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:21

Channel:Brightfield set at:12-Jul-2019 06:53:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 06:53:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:21

Channel configuration set to: GFP: 12-Jul-2019 06:53:22

Channel:GFP set at:12-Jul-2019 06:53:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 06:53:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:23

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:25

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.25

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:26

Channel:Brightfield set at:12-Jul-2019 06:53:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7251. 12-Jul-2019 06:53:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:26

Channel configuration set to: GFP: 12-Jul-2019 06:53:27

Channel:GFP set at:12-Jul-2019 06:53:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7251. 12-Jul-2019 06:53:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:28

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:30

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.85

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:31

Channel:Brightfield set at:12-Jul-2019 06:53:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.2751. 12-Jul-2019 06:53:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:31

Channel configuration set to: GFP: 12-Jul-2019 06:53:33

Channel:GFP set at:12-Jul-2019 06:53:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.2751. 12-Jul-2019 06:53:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:33

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:35

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-7.125

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:36

Channel:Brightfield set at:12-Jul-2019 06:53:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3501. 12-Jul-2019 06:53:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:36

Channel configuration set to: GFP: 12-Jul-2019 06:53:38

Channel:GFP set at:12-Jul-2019 06:53:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3501. 12-Jul-2019 06:53:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:38

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:40

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:42

Channel:Brightfield set at:12-Jul-2019 06:53:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 06:53:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:42

Channel configuration set to: GFP: 12-Jul-2019 06:53:44

Channel:GFP set at:12-Jul-2019 06:53:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 06:53:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:44

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:46

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:49

Channel:Brightfield set at:12-Jul-2019 06:53:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 06:53:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:49

Channel configuration set to: GFP: 12-Jul-2019 06:53:50

Channel:GFP set at:12-Jul-2019 06:53:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 06:53:51

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:51

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:52

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.2

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:53

Channel:Brightfield set at:12-Jul-2019 06:53:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:53:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:53

Channel configuration set to: GFP: 12-Jul-2019 06:53:55

Channel:GFP set at:12-Jul-2019 06:53:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:53:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:55

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:57

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 06:53:59

Channel:Brightfield set at:12-Jul-2019 06:53:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3751. 12-Jul-2019 06:53:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:53:59

Channel configuration set to: GFP: 12-Jul-2019 06:54:00

Channel:GFP set at:12-Jul-2019 06:54:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3751. 12-Jul-2019 06:54:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:54:01

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:54:03

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 06:54:04

Channel:Brightfield set at:12-Jul-2019 06:54:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 12-Jul-2019 06:54:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:54:04

Channel configuration set to: GFP: 12-Jul-2019 06:54:05

Channel:GFP set at:12-Jul-2019 06:54:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9751. 12-Jul-2019 06:54:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:54:06

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:54:08

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-8

Channel configuration set to: Brightfield: 12-Jul-2019 06:54:09

Channel:Brightfield set at:12-Jul-2019 06:54:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 06:54:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:54:10

Channel configuration set to: GFP: 12-Jul-2019 06:54:11

Channel:GFP set at:12-Jul-2019 06:54:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 06:54:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:54:11

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:174 completed at:12-Jul-2019 06:54:13

Time since start of timelapse:51999.847

PFS is locked

Time to next time point:200.9081



------Time point_175------

Memory available for all arrays: 61035425792

Memory used by Matlab: 2265083904

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:57:34

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.3

Channel configuration set to: Brightfield: 12-Jul-2019 06:57:36

Channel:Brightfield set at:12-Jul-2019 06:57:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 06:57:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:57:37

Channel configuration set to: GFP: 12-Jul-2019 06:57:38

Channel:GFP set at:12-Jul-2019 06:57:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 06:57:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:57:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:57:41

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.475

Channel configuration set to: Brightfield: 12-Jul-2019 06:57:42

Channel:Brightfield set at:12-Jul-2019 06:57:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 06:57:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:57:42

Channel configuration set to: GFP: 12-Jul-2019 06:57:43

Channel:GFP set at:12-Jul-2019 06:57:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 06:57:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:57:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:57:46

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.25

Channel configuration set to: Brightfield: 12-Jul-2019 06:57:47

Channel:Brightfield set at:12-Jul-2019 06:57:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 12-Jul-2019 06:57:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:57:47

Channel configuration set to: GFP: 12-Jul-2019 06:57:49

Channel:GFP set at:12-Jul-2019 06:57:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7001. 12-Jul-2019 06:57:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:57:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:57:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.075

Channel configuration set to: Brightfield: 12-Jul-2019 06:57:53

Channel:Brightfield set at:12-Jul-2019 06:57:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 06:57:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:57:54

Channel configuration set to: GFP: 12-Jul-2019 06:57:55

Channel:GFP set at:12-Jul-2019 06:57:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 06:57:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:57:55

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:57:57

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.875

Channel configuration set to: Brightfield: 12-Jul-2019 06:57:58

Channel:Brightfield set at:12-Jul-2019 06:57:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.1001. 12-Jul-2019 06:57:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:57:59

Channel configuration set to: GFP: 12-Jul-2019 06:58:00

Channel:GFP set at:12-Jul-2019 06:58:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 12-Jul-2019 06:58:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:01

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:03

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.2

PFS not locked. Waiting for lock...

PFS took 0.700000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:04

Channel:Brightfield set at:12-Jul-2019 06:58:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.6751. 12-Jul-2019 06:58:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:05

Channel configuration set to: GFP: 12-Jul-2019 06:58:06

Channel:GFP set at:12-Jul-2019 06:58:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7001. 12-Jul-2019 06:58:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:06

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:09

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.5

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:10

Channel:Brightfield set at:12-Jul-2019 06:58:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 06:58:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:10

Channel configuration set to: GFP: 12-Jul-2019 06:58:12

Channel:GFP set at:12-Jul-2019 06:58:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 06:58:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:12

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:14

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.975

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:16

Channel:Brightfield set at:12-Jul-2019 06:58:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9251. 12-Jul-2019 06:58:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:16

Channel configuration set to: GFP: 12-Jul-2019 06:58:17

Channel:GFP set at:12-Jul-2019 06:58:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9501. 12-Jul-2019 06:58:18

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:18

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:20

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.425

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:21

Channel:Brightfield set at:12-Jul-2019 06:58:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 06:58:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:21

Channel configuration set to: GFP: 12-Jul-2019 06:58:22

Channel:GFP set at:12-Jul-2019 06:58:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 06:58:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:23

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:25

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.275

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:26

Channel:Brightfield set at:12-Jul-2019 06:58:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6251. 12-Jul-2019 06:58:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:26

Channel configuration set to: GFP: 12-Jul-2019 06:58:27

Channel:GFP set at:12-Jul-2019 06:58:27

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6251. 12-Jul-2019 06:58:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:28

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:30

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:31

Channel:Brightfield set at:12-Jul-2019 06:58:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 06:58:31

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:31

Channel configuration set to: GFP: 12-Jul-2019 06:58:33

Channel:GFP set at:12-Jul-2019 06:58:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4001. 12-Jul-2019 06:58:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:33

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:35

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:36

Channel:Brightfield set at:12-Jul-2019 06:58:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3001. 12-Jul-2019 06:58:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:37

Channel configuration set to: GFP: 12-Jul-2019 06:58:38

Channel:GFP set at:12-Jul-2019 06:58:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.2751. 12-Jul-2019 06:58:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:38

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:40

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.375

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:42

Channel:Brightfield set at:12-Jul-2019 06:58:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:58:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:42

Channel configuration set to: GFP: 12-Jul-2019 06:58:44

Channel:GFP set at:12-Jul-2019 06:58:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 06:58:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:44

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:46

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-6.175

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:48

Channel:Brightfield set at:12-Jul-2019 06:58:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 06:58:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:48

Channel configuration set to: GFP: 12-Jul-2019 06:58:50

Channel:GFP set at:12-Jul-2019 06:58:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 06:58:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:50

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:52

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:52

Channel:Brightfield set at:12-Jul-2019 06:58:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 06:58:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:53

Channel configuration set to: GFP: 12-Jul-2019 06:58:54

Channel:GFP set at:12-Jul-2019 06:58:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 06:58:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:54

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:57

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-6.025

Channel configuration set to: Brightfield: 12-Jul-2019 06:58:58

Channel:Brightfield set at:12-Jul-2019 06:58:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 06:58:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:58:58

Channel configuration set to: GFP: 12-Jul-2019 06:59:00

Channel:GFP set at:12-Jul-2019 06:59:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 06:59:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:59:00

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:59:02

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-7.075

Channel configuration set to: Brightfield: 12-Jul-2019 06:59:02

Channel:Brightfield set at:12-Jul-2019 06:59:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 06:59:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:59:03

Channel configuration set to: GFP: 12-Jul-2019 06:59:04

Channel:GFP set at:12-Jul-2019 06:59:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 06:59:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:59:04

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 06:59:07

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.825

Channel configuration set to: Brightfield: 12-Jul-2019 06:59:08

Channel:Brightfield set at:12-Jul-2019 06:59:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 06:59:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:59:08

Channel configuration set to: GFP: 12-Jul-2019 06:59:09

Channel:GFP set at:12-Jul-2019 06:59:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 06:59:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 06:59:10

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:175 completed at:12-Jul-2019 06:59:12

Time since start of timelapse:52298.2899

PFS is locked

Time to next time point:202.4666



------Time point_176------

Memory available for all arrays: 61025996800

Memory used by Matlab: 2265083904

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:02:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.125

Channel configuration set to: Brightfield: 12-Jul-2019 07:02:36

Channel:Brightfield set at:12-Jul-2019 07:02:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2251. 12-Jul-2019 07:02:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:02:37

Channel configuration set to: GFP: 12-Jul-2019 07:02:38

Channel:GFP set at:12-Jul-2019 07:02:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 07:02:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:02:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:02:41

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.25

Channel configuration set to: Brightfield: 12-Jul-2019 07:02:42

Channel:Brightfield set at:12-Jul-2019 07:02:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 07:02:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:02:42

Channel configuration set to: GFP: 12-Jul-2019 07:02:43

Channel:GFP set at:12-Jul-2019 07:02:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 07:02:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:02:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:02:46

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.05

Channel configuration set to: Brightfield: 12-Jul-2019 07:02:47

Channel:Brightfield set at:12-Jul-2019 07:02:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 12-Jul-2019 07:02:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:02:47

Channel configuration set to: GFP: 12-Jul-2019 07:02:49

Channel:GFP set at:12-Jul-2019 07:02:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 12-Jul-2019 07:02:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:02:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:02:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-8.2

PFS not locked. Waiting for lock...

PFS took 0.250000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 07:02:52

Channel:Brightfield set at:12-Jul-2019 07:02:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 07:02:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:02:53

Channel configuration set to: GFP: 12-Jul-2019 07:02:54

Channel:GFP set at:12-Jul-2019 07:02:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1751. 12-Jul-2019 07:02:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:02:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:02:57

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-7.9

Channel configuration set to: Brightfield: 12-Jul-2019 07:02:57

Channel:Brightfield set at:12-Jul-2019 07:02:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 07:02:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:02:58

Channel configuration set to: GFP: 12-Jul-2019 07:02:59

Channel:GFP set at:12-Jul-2019 07:02:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5251. 12-Jul-2019 07:02:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:02:59

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.875

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:03

Channel:Brightfield set at:12-Jul-2019 07:03:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 12-Jul-2019 07:03:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:03

Channel configuration set to: GFP: 12-Jul-2019 07:03:04

Channel:GFP set at:12-Jul-2019 07:03:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9001. 12-Jul-2019 07:03:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:07

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:08

Channel:Brightfield set at:12-Jul-2019 07:03:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 07:03:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:09

Channel configuration set to: GFP: 12-Jul-2019 07:03:10

Channel:GFP set at:12-Jul-2019 07:03:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 07:03:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:10

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:12

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.4

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:13

Channel:Brightfield set at:12-Jul-2019 07:03:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 07:03:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:14

Channel configuration set to: GFP: 12-Jul-2019 07:03:15

Channel:GFP set at:12-Jul-2019 07:03:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 07:03:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:15

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:18

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.1

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:18

Channel:Brightfield set at:12-Jul-2019 07:03:18

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1501. 12-Jul-2019 07:03:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:19

Channel configuration set to: GFP: 12-Jul-2019 07:03:20

Channel:GFP set at:12-Jul-2019 07:03:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 07:03:20

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:21

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:23

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:24

Channel:Brightfield set at:12-Jul-2019 07:03:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 07:03:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:24

Channel configuration set to: GFP: 12-Jul-2019 07:03:26

Channel:GFP set at:12-Jul-2019 07:03:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 07:03:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:26

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:28

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.575

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:29

Channel:Brightfield set at:12-Jul-2019 07:03:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6001. 12-Jul-2019 07:03:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:30

Channel configuration set to: GFP: 12-Jul-2019 07:03:31

Channel:GFP set at:12-Jul-2019 07:03:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.6001. 12-Jul-2019 07:03:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:32

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:34

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.825

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:35

Channel:Brightfield set at:12-Jul-2019 07:03:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7001. 12-Jul-2019 07:03:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:35

Channel configuration set to: GFP: 12-Jul-2019 07:03:36

Channel:GFP set at:12-Jul-2019 07:03:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7001. 12-Jul-2019 07:03:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:37

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:39

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.225

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:41

Channel:Brightfield set at:12-Jul-2019 07:03:41

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 07:03:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:41

Channel configuration set to: GFP: 12-Jul-2019 07:03:42

Channel:GFP set at:12-Jul-2019 07:03:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3501. 12-Jul-2019 07:03:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:43

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:45

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:46

Channel:Brightfield set at:12-Jul-2019 07:03:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 07:03:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:47

Channel configuration set to: GFP: 12-Jul-2019 07:03:48

Channel:GFP set at:12-Jul-2019 07:03:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 07:03:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:48

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:51

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.9

PFS not locked. Waiting for lock...

PFS took 0.550000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:52

Channel:Brightfield set at:12-Jul-2019 07:03:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 07:03:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:53

Channel configuration set to: GFP: 12-Jul-2019 07:03:54

Channel:GFP set at:12-Jul-2019 07:03:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5751. 12-Jul-2019 07:03:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:54

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:57

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.8

Channel configuration set to: Brightfield: 12-Jul-2019 07:03:58

Channel:Brightfield set at:12-Jul-2019 07:03:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 07:03:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:03:58

Channel configuration set to: GFP: 12-Jul-2019 07:04:00

Channel:GFP set at:12-Jul-2019 07:04:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 07:04:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:04:00

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:04:02

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.075

Channel configuration set to: Brightfield: 12-Jul-2019 07:04:03

Channel:Brightfield set at:12-Jul-2019 07:04:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 12-Jul-2019 07:04:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:04:03

Channel configuration set to: GFP: 12-Jul-2019 07:04:04

Channel:GFP set at:12-Jul-2019 07:04:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6751. 12-Jul-2019 07:04:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:04:05

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:04:07

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 07:04:09

Channel:Brightfield set at:12-Jul-2019 07:04:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 07:04:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:04:09

Channel configuration set to: GFP: 12-Jul-2019 07:04:10

Channel:GFP set at:12-Jul-2019 07:04:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 07:04:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:04:11

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:176 completed at:12-Jul-2019 07:04:13

Time since start of timelapse:52598.9941

PFS is locked

Time to next time point:201.7637



------Time point_177------

Memory available for all arrays: 61026496512

Memory used by Matlab: 2265083904

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:07:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-12.3

Channel configuration set to: Brightfield: 12-Jul-2019 07:07:35

Channel:Brightfield set at:12-Jul-2019 07:07:35

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 07:07:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:07:36

Channel configuration set to: GFP: 12-Jul-2019 07:07:37

Channel:GFP set at:12-Jul-2019 07:07:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.0501. 12-Jul-2019 07:07:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:07:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:07:40

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.375

Channel configuration set to: Brightfield: 12-Jul-2019 07:07:42

Channel:Brightfield set at:12-Jul-2019 07:07:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4751. 12-Jul-2019 07:07:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:07:42

Channel configuration set to: GFP: 12-Jul-2019 07:07:43

Channel:GFP set at:12-Jul-2019 07:07:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 07:07:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:07:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:07:45

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.025

Channel configuration set to: Brightfield: 12-Jul-2019 07:07:47

Channel:Brightfield set at:12-Jul-2019 07:07:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9251. 12-Jul-2019 07:07:47

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:07:47

Channel configuration set to: GFP: 12-Jul-2019 07:07:49

Channel:GFP set at:12-Jul-2019 07:07:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 12-Jul-2019 07:07:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:07:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:07:51

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.525

Channel configuration set to: Brightfield: 12-Jul-2019 07:07:52

Channel:Brightfield set at:12-Jul-2019 07:07:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 07:07:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:07:53

Channel configuration set to: GFP: 12-Jul-2019 07:07:54

Channel:GFP set at:12-Jul-2019 07:07:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1001. 12-Jul-2019 07:07:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:07:54

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:07:57

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.7

Channel configuration set to: Brightfield: 12-Jul-2019 07:07:58

Channel:Brightfield set at:12-Jul-2019 07:07:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2751. 12-Jul-2019 07:07:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:07:58

Channel configuration set to: GFP: 12-Jul-2019 07:08:00

Channel:GFP set at:12-Jul-2019 07:08:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.2501. 12-Jul-2019 07:08:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:00

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:02

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9

PFS not locked. Waiting for lock...

PFS took 0.650000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:04

Channel:Brightfield set at:12-Jul-2019 07:08:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 07:08:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:04

Channel configuration set to: GFP: 12-Jul-2019 07:08:06

Channel:GFP set at:12-Jul-2019 07:08:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 07:08:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:06

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:09

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:10

Channel:Brightfield set at:12-Jul-2019 07:08:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 07:08:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:10

Channel configuration set to: GFP: 12-Jul-2019 07:08:12

Channel:GFP set at:12-Jul-2019 07:08:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 07:08:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:12

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:14

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:15

Channel:Brightfield set at:12-Jul-2019 07:08:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 07:08:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:15

Channel configuration set to: GFP: 12-Jul-2019 07:08:17

Channel:GFP set at:12-Jul-2019 07:08:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4501. 12-Jul-2019 07:08:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:17

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:19

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.075

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:20

Channel:Brightfield set at:12-Jul-2019 07:08:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 07:08:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:21

Channel configuration set to: GFP: 12-Jul-2019 07:08:22

Channel:GFP set at:12-Jul-2019 07:08:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.3001. 12-Jul-2019 07:08:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:22

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:25

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-7.95

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:26

Channel:Brightfield set at:12-Jul-2019 07:08:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 07:08:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:26

Channel configuration set to: GFP: 12-Jul-2019 07:08:28

Channel:GFP set at:12-Jul-2019 07:08:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9751. 12-Jul-2019 07:08:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:28

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:30

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.45

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:31

Channel:Brightfield set at:12-Jul-2019 07:08:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7001. 12-Jul-2019 07:08:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:32

Channel configuration set to: GFP: 12-Jul-2019 07:08:33

Channel:GFP set at:12-Jul-2019 07:08:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7001. 12-Jul-2019 07:08:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:33

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:36

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.725

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:37

Channel:Brightfield set at:12-Jul-2019 07:08:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 07:08:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:37

Channel configuration set to: GFP: 12-Jul-2019 07:08:38

Channel:GFP set at:12-Jul-2019 07:08:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.7751. 12-Jul-2019 07:08:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:39

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:41

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.15

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:42

Channel:Brightfield set at:12-Jul-2019 07:08:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 07:08:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:43

Channel configuration set to: GFP: 12-Jul-2019 07:08:44

Channel:GFP set at:12-Jul-2019 07:08:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 07:08:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:44

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:47

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:48

Channel:Brightfield set at:12-Jul-2019 07:08:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 07:08:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:48

Channel configuration set to: GFP: 12-Jul-2019 07:08:50

Channel:GFP set at:12-Jul-2019 07:08:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.0251. 12-Jul-2019 07:08:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:50

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:52

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.475

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:54

Channel:Brightfield set at:12-Jul-2019 07:08:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 07:08:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:54

Channel configuration set to: GFP: 12-Jul-2019 07:08:55

Channel:GFP set at:12-Jul-2019 07:08:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 07:08:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:08:56

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:58

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.625

Channel configuration set to: Brightfield: 12-Jul-2019 07:08:59

Channel:Brightfield set at:12-Jul-2019 07:08:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 07:09:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:09:00

Channel configuration set to: GFP: 12-Jul-2019 07:09:01

Channel:GFP set at:12-Jul-2019 07:09:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.7501. 12-Jul-2019 07:09:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:09:01

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:09:04

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 12-Jul-2019 07:09:05

Channel:Brightfield set at:12-Jul-2019 07:09:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 07:09:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:09:05

Channel configuration set to: GFP: 12-Jul-2019 07:09:06

Channel:GFP set at:12-Jul-2019 07:09:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4501. 12-Jul-2019 07:09:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:09:07

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:09:09

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 07:09:10

Channel:Brightfield set at:12-Jul-2019 07:09:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 07:09:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:09:10

Channel configuration set to: GFP: 12-Jul-2019 07:09:12

Channel:GFP set at:12-Jul-2019 07:09:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6251. 12-Jul-2019 07:09:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:09:12

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:177 completed at:12-Jul-2019 07:09:14

Time since start of timelapse:52900.4874

PFS is locked

Time to next time point:200.2717



------Time point_178------

Memory available for all arrays: 61021286400

Memory used by Matlab: 2265083904

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:12:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.325

Channel configuration set to: Brightfield: 12-Jul-2019 07:12:36

Channel:Brightfield set at:12-Jul-2019 07:12:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 07:12:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:12:37

Channel configuration set to: GFP: 12-Jul-2019 07:12:38

Channel:GFP set at:12-Jul-2019 07:12:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.0251. 12-Jul-2019 07:12:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:12:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:12:41

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.35

Channel configuration set to: Brightfield: 12-Jul-2019 07:12:42

Channel:Brightfield set at:12-Jul-2019 07:12:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 07:12:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:12:42

Channel configuration set to: GFP: 12-Jul-2019 07:12:43

Channel:GFP set at:12-Jul-2019 07:12:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 07:12:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:12:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:12:46

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.55

Channel configuration set to: Brightfield: 12-Jul-2019 07:12:48

Channel:Brightfield set at:12-Jul-2019 07:12:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 12-Jul-2019 07:12:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:12:49

Channel configuration set to: GFP: 12-Jul-2019 07:12:50

Channel:GFP set at:12-Jul-2019 07:12:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.3501. 12-Jul-2019 07:12:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:12:50

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:12:53

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-9.75

Channel configuration set to: Brightfield: 12-Jul-2019 07:12:54

Channel:Brightfield set at:12-Jul-2019 07:12:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 07:12:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:12:55

Channel configuration set to: GFP: 12-Jul-2019 07:12:56

Channel:GFP set at:12-Jul-2019 07:12:56

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7751. 12-Jul-2019 07:12:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:12:56

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:12:59

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.925

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:00

Channel:Brightfield set at:12-Jul-2019 07:13:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0751. 12-Jul-2019 07:13:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:01

Channel configuration set to: GFP: 12-Jul-2019 07:13:02

Channel:GFP set at:12-Jul-2019 07:13:02

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 12-Jul-2019 07:13:02

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:02

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:04

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:05

Channel:Brightfield set at:12-Jul-2019 07:13:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 12-Jul-2019 07:13:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:06

Channel configuration set to: GFP: 12-Jul-2019 07:13:07

Channel:GFP set at:12-Jul-2019 07:13:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.4751. 12-Jul-2019 07:13:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:07

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:10

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.25

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:11

Channel:Brightfield set at:12-Jul-2019 07:13:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 07:13:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:12

Channel configuration set to: GFP: 12-Jul-2019 07:13:13

Channel:GFP set at:12-Jul-2019 07:13:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5501. 12-Jul-2019 07:13:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:13

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:15

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-7.875

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:17

Channel:Brightfield set at:12-Jul-2019 07:13:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0501. 12-Jul-2019 07:13:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:17

Channel configuration set to: GFP: 12-Jul-2019 07:13:19

Channel:GFP set at:12-Jul-2019 07:13:19

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 07:13:19

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:19

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:21

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.2

PFS not locked. Waiting for lock...

PFS took 0.100000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:22

Channel:Brightfield set at:12-Jul-2019 07:13:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 07:13:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:22

Channel configuration set to: GFP: 12-Jul-2019 07:13:24

Channel:GFP set at:12-Jul-2019 07:13:24

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.2751. 12-Jul-2019 07:13:24

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:24

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:26

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.025

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:27

Channel:Brightfield set at:12-Jul-2019 07:13:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 07:13:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:28

Channel configuration set to: GFP: 12-Jul-2019 07:13:29

Channel:GFP set at:12-Jul-2019 07:13:29

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.9251. 12-Jul-2019 07:13:30

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:30

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:32

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.6

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:33

Channel:Brightfield set at:12-Jul-2019 07:13:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5501. 12-Jul-2019 07:13:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:33

Channel configuration set to: GFP: 12-Jul-2019 07:13:34

Channel:GFP set at:12-Jul-2019 07:13:34

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5501. 12-Jul-2019 07:13:35

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:35

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:37

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:39

Channel:Brightfield set at:12-Jul-2019 07:13:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 07:13:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:39

Channel configuration set to: GFP: 12-Jul-2019 07:13:40

Channel:GFP set at:12-Jul-2019 07:13:40

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 07:13:41

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:41

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:43

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:44

Channel:Brightfield set at:12-Jul-2019 07:13:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 07:13:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:44

Channel configuration set to: GFP: 12-Jul-2019 07:13:46

Channel:GFP set at:12-Jul-2019 07:13:46

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2501. 12-Jul-2019 07:13:46

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:46

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:48

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.875

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:50

Channel:Brightfield set at:12-Jul-2019 07:13:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 07:13:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:50

Channel configuration set to: GFP: 12-Jul-2019 07:13:51

Channel:GFP set at:12-Jul-2019 07:13:52

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8501. 12-Jul-2019 07:13:52

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:52

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:54

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 12-Jul-2019 07:13:55

Channel:Brightfield set at:12-Jul-2019 07:13:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 07:13:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:56

Channel configuration set to: GFP: 12-Jul-2019 07:13:57

Channel:GFP set at:12-Jul-2019 07:13:57

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 07:13:58

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:13:58

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:14:00

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.825

Channel configuration set to: Brightfield: 12-Jul-2019 07:14:01

Channel:Brightfield set at:12-Jul-2019 07:14:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 07:14:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:14:01

Channel configuration set to: GFP: 12-Jul-2019 07:14:03

Channel:GFP set at:12-Jul-2019 07:14:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5751. 12-Jul-2019 07:14:03

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:14:03

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:14:05

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-6.425

Channel configuration set to: Brightfield: 12-Jul-2019 07:14:06

Channel:Brightfield set at:12-Jul-2019 07:14:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 07:14:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:14:07

Channel configuration set to: GFP: 12-Jul-2019 07:14:08

Channel:GFP set at:12-Jul-2019 07:14:08

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.4001. 12-Jul-2019 07:14:08

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:14:08

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:14:10

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.675

Channel configuration set to: Brightfield: 12-Jul-2019 07:14:12

Channel:Brightfield set at:12-Jul-2019 07:14:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 07:14:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:14:12

Channel configuration set to: GFP: 12-Jul-2019 07:14:13

Channel:GFP set at:12-Jul-2019 07:14:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 07:14:14

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:14:14

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:178 completed at:12-Jul-2019 07:14:16

Time since start of timelapse:53201.9334

PFS is locked

Time to next time point:198.8269



------Time point_179------

Memory available for all arrays: 61025591296

Memory used by Matlab: 2265083904

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:17:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-8.425

Channel configuration set to: Brightfield: 12-Jul-2019 07:17:36

Channel:Brightfield set at:12-Jul-2019 07:17:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7751. 12-Jul-2019 07:17:36

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:17:36

Channel configuration set to: GFP: 12-Jul-2019 07:17:38

Channel:GFP set at:12-Jul-2019 07:17:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.7501. 12-Jul-2019 07:17:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:17:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:17:41

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.3

Channel configuration set to: Brightfield: 12-Jul-2019 07:17:42

Channel:Brightfield set at:12-Jul-2019 07:17:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 07:17:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:17:42

Channel configuration set to: GFP: 12-Jul-2019 07:17:44

Channel:GFP set at:12-Jul-2019 07:17:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6001. 12-Jul-2019 07:17:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:17:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:17:46

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-9.575

PFS not locked. Waiting for lock...

PFS took 0.150000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 07:17:47

Channel:Brightfield set at:12-Jul-2019 07:17:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 07:17:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:17:48

Channel configuration set to: GFP: 12-Jul-2019 07:17:49

Channel:GFP set at:12-Jul-2019 07:17:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6251. 12-Jul-2019 07:17:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:17:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:17:52

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.575

Channel configuration set to: Brightfield: 12-Jul-2019 07:17:53

Channel:Brightfield set at:12-Jul-2019 07:17:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 07:17:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:17:54

Channel configuration set to: GFP: 12-Jul-2019 07:17:55

Channel:GFP set at:12-Jul-2019 07:17:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1251. 12-Jul-2019 07:17:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:17:55

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:17:58

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-9.95

Channel configuration set to: Brightfield: 12-Jul-2019 07:17:59

Channel:Brightfield set at:12-Jul-2019 07:17:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 07:17:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:17:59

Channel configuration set to: GFP: 12-Jul-2019 07:18:01

Channel:GFP set at:12-Jul-2019 07:18:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.0251. 12-Jul-2019 07:18:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:01

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:03

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.925

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:04

Channel:Brightfield set at:12-Jul-2019 07:18:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.8001. 12-Jul-2019 07:18:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:05

Channel configuration set to: GFP: 12-Jul-2019 07:18:06

Channel:GFP set at:12-Jul-2019 07:18:06

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7751. 12-Jul-2019 07:18:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:06

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:08

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.325

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:10

Channel:Brightfield set at:12-Jul-2019 07:18:10

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.5001. 12-Jul-2019 07:18:10

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:10

Channel configuration set to: GFP: 12-Jul-2019 07:18:12

Channel:GFP set at:12-Jul-2019 07:18:12

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4751. 12-Jul-2019 07:18:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:12

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:14

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.675

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:15

Channel:Brightfield set at:12-Jul-2019 07:18:15

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 07:18:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:16

Channel configuration set to: GFP: 12-Jul-2019 07:18:17

Channel:GFP set at:12-Jul-2019 07:18:17

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 07:18:17

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:17

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:20

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.25

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:21

Channel:Brightfield set at:12-Jul-2019 07:18:21

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 07:18:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:21

Channel configuration set to: GFP: 12-Jul-2019 07:18:23

Channel:GFP set at:12-Jul-2019 07:18:23

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 07:18:23

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:23

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:25

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.125

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:26

Channel:Brightfield set at:12-Jul-2019 07:18:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8251. 12-Jul-2019 07:18:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:26

Channel configuration set to: GFP: 12-Jul-2019 07:18:28

Channel:GFP set at:12-Jul-2019 07:18:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8251. 12-Jul-2019 07:18:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:28

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:30

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.65

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:31

Channel:Brightfield set at:12-Jul-2019 07:18:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5001. 12-Jul-2019 07:18:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:32

Channel configuration set to: GFP: 12-Jul-2019 07:18:33

Channel:GFP set at:12-Jul-2019 07:18:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.5001. 12-Jul-2019 07:18:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:33

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:35

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-7.175

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:36

Channel:Brightfield set at:12-Jul-2019 07:18:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3001. 12-Jul-2019 07:18:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:37

Channel configuration set to: GFP: 12-Jul-2019 07:18:38

Channel:GFP set at:12-Jul-2019 07:18:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.3001. 12-Jul-2019 07:18:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:38

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:41

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-5.25

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:42

Channel:Brightfield set at:12-Jul-2019 07:18:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 12-Jul-2019 07:18:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:42

Channel configuration set to: GFP: 12-Jul-2019 07:18:43

Channel:GFP set at:12-Jul-2019 07:18:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.2501. 12-Jul-2019 07:18:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:44

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:46

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.925

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:48

Channel:Brightfield set at:12-Jul-2019 07:18:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 07:18:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:48

Channel configuration set to: GFP: 12-Jul-2019 07:18:49

Channel:GFP set at:12-Jul-2019 07:18:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.8001. 12-Jul-2019 07:18:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:50

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:51

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.625

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:53

Channel:Brightfield set at:12-Jul-2019 07:18:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7251. 12-Jul-2019 07:18:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:53

Channel configuration set to: GFP: 12-Jul-2019 07:18:54

Channel:GFP set at:12-Jul-2019 07:18:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7501. 12-Jul-2019 07:18:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:55

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:57

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.9

Channel configuration set to: Brightfield: 12-Jul-2019 07:18:58

Channel:Brightfield set at:12-Jul-2019 07:18:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 07:18:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:18:59

Channel configuration set to: GFP: 12-Jul-2019 07:19:00

Channel:GFP set at:12-Jul-2019 07:19:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5001. 12-Jul-2019 07:19:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:19:00

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:19:03

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.275

Channel configuration set to: Brightfield: 12-Jul-2019 07:19:04

Channel:Brightfield set at:12-Jul-2019 07:19:04

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5001. 12-Jul-2019 07:19:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:19:04

Channel configuration set to: GFP: 12-Jul-2019 07:19:05

Channel:GFP set at:12-Jul-2019 07:19:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5001. 12-Jul-2019 07:19:06

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:19:06

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:19:08

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-7.75

Channel configuration set to: Brightfield: 12-Jul-2019 07:19:09

Channel:Brightfield set at:12-Jul-2019 07:19:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 07:19:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:19:09

Channel configuration set to: GFP: 12-Jul-2019 07:19:11

Channel:GFP set at:12-Jul-2019 07:19:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6001. 12-Jul-2019 07:19:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:19:11

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:179 completed at:12-Jul-2019 07:19:13

Time since start of timelapse:53499.3284

PFS is locked

Time to next time point:201.4334



------Time point_180------

Memory available for all arrays: 61027254272

Memory used by Matlab: 2265346048

Position:1,URA7_young001

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:22:35

Moved to X:6998.4, Y:6827.4

Call to readDrift after moving to position1

Setting PFS offset to :165.85

readDrift script. Reference z position is:3391.3251

readDrift script. Cumulative drift is:-10.2

Channel configuration set to: Brightfield: 12-Jul-2019 07:22:36

Channel:Brightfield set at:12-Jul-2019 07:22:36

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 12-Jul-2019 07:22:37

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:22:37

Channel configuration set to: GFP: 12-Jul-2019 07:22:38

Channel:GFP set at:12-Jul-2019 07:22:38

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.1501. 12-Jul-2019 07:22:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:22:38

Position:2,URA7_young002

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:22:41

Moved to X:7246.7, Y:6904.4

Call to readDrift after moving to position2

Setting PFS offset to :169.7

readDrift script. Reference z position is:3392.9001

readDrift script. Cumulative drift is:-9.3

Channel configuration set to: Brightfield: 12-Jul-2019 07:22:42

Channel:Brightfield set at:12-Jul-2019 07:22:42

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 07:22:42

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:22:42

Channel configuration set to: GFP: 12-Jul-2019 07:22:44

Channel:GFP set at:12-Jul-2019 07:22:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.5501. 12-Jul-2019 07:22:44

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:22:44

Position:3,URA7_young003

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:22:46

Moved to X:7614.9, Y:6835.5

Call to readDrift after moving to position3

Setting PFS offset to :159.35

readDrift script. Reference z position is:3392.9251

readDrift script. Cumulative drift is:-10.025

Channel configuration set to: Brightfield: 12-Jul-2019 07:22:47

Channel:Brightfield set at:12-Jul-2019 07:22:47

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.8751. 12-Jul-2019 07:22:48

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:22:48

Channel configuration set to: GFP: 12-Jul-2019 07:22:49

Channel:GFP set at:12-Jul-2019 07:22:49

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.9001. 12-Jul-2019 07:22:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:22:49

Position:4,URA7_young004

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:22:52

Moved to X:7475.6, Y:7139.7

Call to readDrift after moving to position4

Setting PFS offset to :158.25

readDrift script. Reference z position is:3391.6501

readDrift script. Cumulative drift is:-10.675

Channel configuration set to: Brightfield: 12-Jul-2019 07:22:53

Channel:Brightfield set at:12-Jul-2019 07:22:53

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 07:22:53

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:22:53

Channel configuration set to: GFP: 12-Jul-2019 07:22:54

Channel:GFP set at:12-Jul-2019 07:22:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.9751. 12-Jul-2019 07:22:55

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:22:55

Position:5,URA7_young005

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:22:57

Moved to X:7141.1, Y:7059.1

Call to readDrift after moving to position5

Setting PFS offset to :153.55

readDrift script. Reference z position is:3389.9751

readDrift script. Cumulative drift is:-10

Channel configuration set to: Brightfield: 12-Jul-2019 07:22:58

Channel:Brightfield set at:12-Jul-2019 07:22:58

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 07:22:59

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:22:59

Channel configuration set to: GFP: 12-Jul-2019 07:23:00

Channel:GFP set at:12-Jul-2019 07:23:00

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.9751. 12-Jul-2019 07:23:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:00

Position:6,URA7_young006

Position group:1

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:03

Moved to X:6930.9, Y:6908.7

Call to readDrift after moving to position6

Setting PFS offset to :160.65

readDrift script. Reference z position is:3389.6751

readDrift script. Cumulative drift is:-9.125

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:03

Channel:Brightfield set at:12-Jul-2019 07:23:03

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 07:23:04

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:04

Channel configuration set to: GFP: 12-Jul-2019 07:23:05

Channel:GFP set at:12-Jul-2019 07:23:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.7501. 12-Jul-2019 07:23:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:05

Position:7,URA8_old007

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:08

Moved to X:6869.7, Y:5166.9

Call to readDrift after moving to position7

Setting PFS offset to :162.15

readDrift script. Reference z position is:3387.8751

readDrift script. Cumulative drift is:-6.1

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:09

Channel:Brightfield set at:12-Jul-2019 07:23:09

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.6751. 12-Jul-2019 07:23:09

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:09

Channel configuration set to: GFP: 12-Jul-2019 07:23:11

Channel:GFP set at:12-Jul-2019 07:23:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.7001. 12-Jul-2019 07:23:11

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:11

Position:8,URA8_old008

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:13

Moved to X:6925.1, Y:5285.7

Call to readDrift after moving to position8

Setting PFS offset to :152.75

readDrift script. Reference z position is:3387.9251

readDrift script. Cumulative drift is:-6.525

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:14

Channel:Brightfield set at:12-Jul-2019 07:23:14

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4001. 12-Jul-2019 07:23:15

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:15

Channel configuration set to: GFP: 12-Jul-2019 07:23:16

Channel:GFP set at:12-Jul-2019 07:23:16

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.3751. 12-Jul-2019 07:23:16

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:16

Position:9,URA8_old009

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:19

Moved to X:6716.5, Y:5425.1

Call to readDrift after moving to position9

Setting PFS offset to :161

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-7.45

PFS not locked. Waiting for lock...

PFS took 0.600000 seconds to lock.

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:20

Channel:Brightfield set at:12-Jul-2019 07:23:20

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 07:23:21

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:21

Channel configuration set to: GFP: 12-Jul-2019 07:23:22

Channel:GFP set at:12-Jul-2019 07:23:22

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3380.7501. 12-Jul-2019 07:23:22

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:22

Position:10,URA8_old010

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:25

Moved to X:6660.3, Y:5187

Call to readDrift after moving to position10

Setting PFS offset to :159.5

readDrift script. Reference z position is:3386.9251

readDrift script. Cumulative drift is:-8.05

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:26

Channel:Brightfield set at:12-Jul-2019 07:23:26

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8751. 12-Jul-2019 07:23:26

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:26

Channel configuration set to: GFP: 12-Jul-2019 07:23:28

Channel:GFP set at:12-Jul-2019 07:23:28

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.8751. 12-Jul-2019 07:23:28

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:28

Position:11,URA8_old011

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:30

Moved to X:6399, Y:5321.4

Call to readDrift after moving to position11

Setting PFS offset to :160.3

readDrift script. Reference z position is:3386.1501

readDrift script. Cumulative drift is:-7.725

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:31

Channel:Brightfield set at:12-Jul-2019 07:23:31

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4501. 12-Jul-2019 07:23:32

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:32

Channel configuration set to: GFP: 12-Jul-2019 07:23:33

Channel:GFP set at:12-Jul-2019 07:23:33

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3378.4501. 12-Jul-2019 07:23:33

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:33

Position:12,URA8_old012

Position group:2

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:36

Moved to X:6255.4, Y:5173.5

Call to readDrift after moving to position12

Setting PFS offset to :165.95

readDrift script. Reference z position is:3385.4751

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:37

Channel:Brightfield set at:12-Jul-2019 07:23:37

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 07:23:38

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:38

Channel configuration set to: GFP: 12-Jul-2019 07:23:39

Channel:GFP set at:12-Jul-2019 07:23:39

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3379.1251. 12-Jul-2019 07:23:39

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:39

Position:13,URA8_young013

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:41

Moved to X:6959.3, Y:6716.3

Call to readDrift after moving to position13

Setting PFS offset to :160.15

readDrift script. Reference z position is:3387.5251

readDrift script. Cumulative drift is:-6.35

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:43

Channel:Brightfield set at:12-Jul-2019 07:23:43

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 07:23:43

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:43

Channel configuration set to: GFP: 12-Jul-2019 07:23:44

Channel:GFP set at:12-Jul-2019 07:23:44

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.2001. 12-Jul-2019 07:23:45

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:45

Position:14,URA8_young014

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:47

Moved to X:7001.5, Y:6981.2

Call to readDrift after moving to position14

Setting PFS offset to :160.55

readDrift script. Reference z position is:3387.8251

readDrift script. Cumulative drift is:-5.85

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:48

Channel:Brightfield set at:12-Jul-2019 07:23:48

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9751. 12-Jul-2019 07:23:49

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:49

Channel configuration set to: GFP: 12-Jul-2019 07:23:50

Channel:GFP set at:12-Jul-2019 07:23:50

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.9501. 12-Jul-2019 07:23:50

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:50

Position:15,URA8_young015

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:53

Moved to X:7258.2, Y:7099.3

Call to readDrift after moving to position15

Setting PFS offset to :158.9

readDrift script. Reference z position is:3388.3751

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:54

Channel:Brightfield set at:12-Jul-2019 07:23:54

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 07:23:54

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:54

Channel configuration set to: GFP: 12-Jul-2019 07:23:55

Channel:GFP set at:12-Jul-2019 07:23:55

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3381.4251. 12-Jul-2019 07:23:56

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:23:56

Position:16,URA8_young016

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:58

Moved to X:7327.7, Y:6846.9

Call to readDrift after moving to position16

Setting PFS offset to :163.35

readDrift script. Reference z position is:3389.4251

readDrift script. Cumulative drift is:-5.75

Channel configuration set to: Brightfield: 12-Jul-2019 07:23:59

Channel:Brightfield set at:12-Jul-2019 07:23:59

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 07:24:00

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:24:00

Channel configuration set to: GFP: 12-Jul-2019 07:24:01

Channel:GFP set at:12-Jul-2019 07:24:01

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3383.6501. 12-Jul-2019 07:24:01

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:24:01

Position:17,URA8_young017

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:24:04

Moved to X:7595, Y:6831.9

Call to readDrift after moving to position17

Setting PFS offset to :165.1

readDrift script. Reference z position is:3389.7751

readDrift script. Cumulative drift is:-5.175

Channel configuration set to: Brightfield: 12-Jul-2019 07:24:05

Channel:Brightfield set at:12-Jul-2019 07:24:05

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.6001. 12-Jul-2019 07:24:05

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:24:05

Channel configuration set to: GFP: 12-Jul-2019 07:24:07

Channel:GFP set at:12-Jul-2019 07:24:07

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3384.5501. 12-Jul-2019 07:24:07

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:24:07

Position:18,URA8_young018

Position group:3

Channel configuration set to: Brightfield: 12-Jul-2019 07:24:09

Moved to X:7640.5, Y:6986.2

Call to readDrift after moving to position18

Setting PFS offset to :155.4

readDrift script. Reference z position is:3389.3501

readDrift script. Cumulative drift is:-6.95

Channel configuration set to: Brightfield: 12-Jul-2019 07:24:11

Channel:Brightfield set at:12-Jul-2019 07:24:11

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.4001. 12-Jul-2019 07:24:12

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:24:12

Channel configuration set to: GFP: 12-Jul-2019 07:24:13

Channel:GFP set at:12-Jul-2019 07:24:13

Exposure time:30ms

Exposure time:30ms

captureStack (Batgirl). Z drive position before stack capture is: 3382.3501. 12-Jul-2019 07:24:13

captureStack (Batgirl). Starting Z stage position is: -1.7. 12-Jul-2019 07:24:13

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:Brightfield

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Position group_1. Channel:GFP

Maximum intensity measured in position group_1_is:0

Channel does not use Smart EM camera mode. Settings remain unchanged.

Timepoint:180 completed at:12-Jul-2019 07:24:16

Time since start of timelapse:53801.9346

PFS is locked

All pumps switched off as acquisition complete.

Experiment completed

